Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,620,256	C→T	G218G (GGC→GGT)	deoB →	phosphopentomutase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,620,256	0	C	T	94.0%	136.8 / NA	52	G218G (GGC→GGT)	deoB	phosphopentomutase
Reads supporting (aligned to +/- strand):  ref base C (0/1);  major base T (23/24);  minor base G (3/0);  total (27/25)
Fisher's exact test for biased strand distribution p-value = 2.36e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAACTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCATGAAGAAACTTTCGGTCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAATATCGGTCGTGTTATCGCTCGTCCGTTTATCGGCGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGT‑TGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACAC‑GGCCAGG‑TGGTTTCTGTCGGTAAAATTGCGGACATCTACGCCAACTGCGGTATCACCAAAAAAGTGAAAGCGACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGT  >  NC_000913/4620014‑4620495                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                   cggCCGGTTTACCCCGGCACCTGCCACTCTTCCGGTACGGTCATTCTGGATCAGCGGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGc                                                                                                                                                                                                                                            <  2:388624/249‑1 (MQ=255)           acctcgtaacctgccaacctTCCGGTACGGTCATTCTGGATCAGCTGGGCGAAGAGCACATGAAAACCGTCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCTTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACtt                                                                                                                                                                                                                                   <  2:225887/232‑1 (MQ=255)                ggTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAGCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCg                                                                                                                                                                                                                             <  1:99616/251‑1 (MQ=255)                 gTAACTGCCACTCTGCCGGTACGGTCATTCTGGATCAGCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGt                                                                                                                                                                                                                            <  1:233809/251‑1 (MQ=255)                        ccACACTTCCGGTACTGTAATTATGGATCAGCTGGGCAAAGAACACATGAAAACCGCCAAGCCGAGTTTCTATACCTCCGCTGACTCCGTGTCCCAGATTGCCTGCCACGAAGGCACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCATCGGCGGCTACAACATCTGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCgtta                                                                                                                                                                                                                     <  2:116511/251‑4 (MQ=255)                              ttCCGGTACGGTCATTCTGGATCAGCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGCTTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGGGACAAAGACGGTAACTTCCTGCGTACCGGTaaaccac                                                                                                                                                                                                               >  2:78625/1‑246 (MQ=255)                                ccGGTACGGTCATTCTGGATCAGCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCAc                                                                                                                                                                                                             <  1:258111/251‑1 (MQ=255)                                ccGGTACGGGCATTCTGGAGTAGTGGGGCTAAGAGCCCTTGAAAACCGTCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCCCGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGTCGGTA‑CTTCCAGCGTACCGGTAACCGTCACg                                                                                                                                                                                                            <  2:373706/251‑1 (MQ=255)                                 cGGTACGGTCATTCTGGATCAGCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGGGACAAAGCCGGTAACTTCCATCGTACCGGTAACCGTCAc                                                                                                                                                                                                             >  1:373389/1‑250 (MQ=255)                                                gtaccaggggggCGAAGAGCCCATGACAACCGTCAAGCCGTTTTTCTAAACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGT‑TGAg                                                                                                                                                                                             <  2:340907/241‑1 (MQ=255)                                                      gcggggCGAAGAGCACAGGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGAGTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGT‑TGAGCCGCCAg                                                                                                                                                                                      <  2:7576/248‑1 (MQ=255)                                                          ggCGAAGAGCACATGAAACCCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGT‑TGAGCCGCCAGCacc                                                                                                                                                                                  <  1:448435/251‑1 (MQ=255)                                                            cGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCAACAACATCGGGCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCAAGACCTTGCTGT‑TGAgacgcccgaccccg                                                                                                                                                                                >  2:441806/1‑243 (MQ=255)                                                                 ggccaaTGAAAACCGGCAAGCCGATTTTCTGTACCCCCGCTGACTCCGTGTTCCAGAGTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAGCCGTCACGACCTGGCTGT‑TGAGCCGCCAGCACCGACCGtg                                                                                                                                                                           <  2:231622/246‑1 (MQ=255)                                                                 agCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGATGACTCCGTGTTCCAGATTGCCTGCCACGACGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGCCGGACACAACCTCGGACGTGTTATCGCTCGTCCCATTATCGGTGACAAAGCCGGTAAAATCAAGCGTACCGGTAACCGTCACCACCTGGccatttaggaccccacaaccaccgtg                                                                                                                                                                            >  2:67650/1‑226 (MQ=255)                                                                    acaTGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACATCATCGGTCGTGTTATCGCTCGTCCGTTTAGCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGT‑TGAGCCGCCAGCACCGACCGtgctg                                                                                                                                                                        <  1:246634/251‑1 (MQ=255)                                                                           aaCCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTACGATTATCGGTGACAAAGCCGGTAACTTCCACCGTACCGGGAACCGTCGCGACCTTGCAGT‑TGAGCCGCCAGCCCAGACCGCGCTGCCGaaa                                                                                                                                                                  >  2:484740/1‑250 (MQ=255)                                                                              cGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGGTAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGT‑TGAGCCGCCAGCACCGACCGTGCTGCAGAAACTgg                                                                                                                                                              <  2:126720/251‑1 (MQ=255)                                                                               ggCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCAGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGAGACAAAGCCGGGAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGT‑TGAGCCGCCAGCCCCGCCCGTGCTGCAGAAACTggg                                                                                                                                                             >  1:67907/1‑250 (MQ=255)                                                                                   aGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGCCCAACGGCCGCCACAACATCGGTCGTGATATCGCTCGTCCGTTTATCCGTGACAAAGCCGGTAACTTCCAGCGTACCGGAAACCGTCACGACCTGGATGT‑TGAGCCGCCAGCACCGACCGAGCTGCAGAAACTGGTtgat                                                                                                                                                         >  1:73554/1‑251 (MQ=255)                                                                                          tttcattaCCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGTCCTGGCTGT‑TGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGaaaaa                                                                                                                                                   <  2:397852/244‑1 (MQ=255)                                                                                          tttCTATCCCTCCGCTGGCTCCGCGTTCCAGCTTGCCTGCAAAGAAGGAACCTTTGGCCTGGCTACACTCTCCGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCACCGGCGGCTACAACCTCGGACGTGGTTTCGCTAGTCCGTTTCTCGGTTACCAAGCCGGGCACTTCCCGCGGAGCTGGCACCGTCCCGACCAGGC‑GTGTGTGCCGCCAGCCCCGCCCGGGCTGCAgctacttgtgggagaacgac                                                                                                                                                  >  2:220221/1‑232 (MQ=255)                                                                                             ccttccctcccatggcTCCGTGATCCAGATGGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACAACCTGTGCGATATCGTCCGTGAAGAGCGGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGCAACTTCCAGCGTACCGGGAACCGACACGACCTGGATGT‑TGAGCCGCCAGCCCCGACCGTGCTTCAGAAACTTGTTGATGAAAAACAC‑g                                                                                                                                              <  2:31290/236‑1 (MQ=255)                                                                                                     ccGCTGACTCCGTGTTCCAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGT‑TGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACAC‑GGTCAgg‑t                                                                                                                                      <  1:78625/250‑1 (MQ=255)                                                                                                       gCTGACTCCGTGTTCCAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGT‑TGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACAC‑GGTCAgg‑tggt                                                                                                                                   >  1:307761/1‑251 (MQ=255)                                                                                                          tatcccGTGTTCCAGTTTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGACCTGTGCGAAACCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGT‑TGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACAC‑GGTCAGG‑TGGttt                                                                                                                                 <  2:503852/246‑1 (MQ=255)                                                                                                              ccGTGTTCCAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTAAAACATCGGTCGGGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACAACCTGGCTGT‑TGAGCCGCCAGCACCGGCCGTGCTGCAGAAACTGGTTGATGGAAA‑CACCGGGCAGG‑GGGTTTCTGTc                                                                                                                            >  1:52598/1‑251 (MQ=255)                                                                                                                     ccAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGT‑TGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACAC‑GGTCAGG‑GGGTTTCTGTCGGTaaa                                                                                                                      >  1:233418/1‑250 (MQ=255)                                                                                                                      cAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCCCGACCTGGCTGT‑TGGACCCCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGGAAAACAC‑GGTCAGGGTGGTT‑CTTTCGGGAAAAt                                                                                                                    >  2:393229/1‑251 (MQ=255)                                                                                                                          ttgccgtccATGAAGAAACTTTCGGTCTGGATAACCTCTCCGAACTGTGCGAAATCGCCCTTGAAGAGCTGACCAACGGCGGCGACAATATCGGTCGTGTTATCGCTCGTCCGTTTATCGGCGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGT‑TGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACAC‑GGCCAGG‑TGGTTTCTGTCGGTAAAATTGc                                                                                                                 <  2:100512/243‑1 (MQ=255)                                                                                                                                   acgaaggatccttgggccTGGACAAACTCTACGAATTGTGCGAAATCGCCCGTGACGAGCGGACCACAGGCGGCTTTAATATCGGTGGTGTTATCGCTCGTCCCTTTATCGGTGAAAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACTGCCTGGCTGT‑TGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACAC‑GGTCAGG‑TGGTTTCTGTCGGTAAAATTGCAGACATCTAc                                                                                                       <  2:377518/234‑1 (MQ=255)                                                                                                                                                        aTAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGACGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTTACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGT‑TGCGCCGCCAGCCCCGACCGTGCTGCAGAAACTGGTTGATGAAAAACAC‑GGTCAAG‑GGGTTTATGTCGGTAAAATTGCAGGCATCTAAGCCCACAGCGGTAttcaca                                                                                    >  2:305268/1‑244 (MQ=255)                                                                                                                                                         tAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGT‑TGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACAC‑GGTCAGG‑TGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCa                                                                                    >  1:420452/1‑248 (MQ=255)                                                                                                                                                           aaCTCTACGAACTGTGCGAAATCGCCCGTGAAGCGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGT‑TGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACAC‑GGTCAGG‑TGGTTTCTGTCGGGAAAATTGCAGACCTCTACGCCAACTGCGGTATCAcaaagaa                                                                                >  1:163271/1‑244 (MQ=255)                                                                                                                                                            aCTCTACGAACTGTGCGAAATCGCCCGTGACGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTACCTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGT‑TGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACAC‑GGTCAGG‑TGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAg                                                                              <  1:59256/251‑1 (MQ=255)                                                                                                                                                              tctACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGT‑TGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACAC‑GGTCAGG‑TGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCCACTGCGGTATCACCAAGAAAGTg                                                                            >  1:111492/1‑251 (MQ=255)                                                                                                                                                                tACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTCCAACCTCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGT‑TGAGCCGCCAGCACCGTCCGTGCTGCCGAAACTGGTTGATGAAAAACAC‑GGTCAGG‑TGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGa                                                                           >  1:189518/1‑250 (MQ=255)                                                                                                                                                                  cGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGGGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGT‑TGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGGTGATGAAAAAAAC‑GGTCAGG‑TGGTTTCTGTCGGTAAAATTGCAGACATCTTAGCCAACTGCGGTATCACCAAGAAAGTGaaa                                                                         >  1:409257/1‑250 (MQ=255)                                                                                                                                                                  cGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGGGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGT‑TGAGCCGCCCGCACCGACCGGGCTGCAGAAACTGGGTGATGAAAAACAC‑GGTCAGG‑TGGTTTCTGTCGGGAAAATTGCCGACATCTACCCCCACTGCGGGATCACCACGAAAGTGaaa                                                                         >  2:113761/1‑250 (MQ=255)                                                                                                                                                                  cGAACTGTGCGAAATCGCCCGTGAAGAGCGGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGT‑TGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACAC‑GGTCAGG‑TGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAg                                                                        <  2:189518/251‑1 (MQ=255)                                                                                                                                                                    accTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCCCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGT‑TGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACAC‑GGTCAGG‑TGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGca                                                                      <  2:17005/249‑2 (MQ=255)                                                                                                                                                                     aCTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACCACATCGGTCGTTTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGT‑TGAGCCGCCAGCCCCGACCGTGCTGCAGAAACTGGTTGATGAGAAAAAC‑GGTCAGG‑TGGTTTCTGTCGGGAAAATTGCAGACATCATCGCCAACTGCGGTATCACCacgaaaaggaaagca                                                                      >  2:258683/1‑236 (MQ=255)                                                                                                                                                                                  gCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGGCGTGTTATCGCCCGTCCGTTTATCGGTGACACAGCCGGTAACTTCCAGCGTACCGGTAAACGTCACGACCTGGCTGT‑TGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACAC‑GGTCAGG‑TGGTTTCTGTCGGTAAAATTGCAGACATCTACGACAACTGCGGTATCACCAAGAAAGGTAAAGCAACTGGcacggaagc                                                        >  2:155146/1‑243 (MQ=255)                                                                                                                                                                                        gAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTACGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGT‑TGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACAC‑GGTCAGG‑TGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGtt                                                  >  1:447211/1‑251 (MQ=255)                                                                                                                                                                                           gagcggaacaagggaggcTAAAAAATCGGTCGGGTTATCGCGAGGCAGTGTAACGGTGAAAAAGACGGTAACGTCCAGCGTACCGGTAACCGGAACGACCGGGCGGT‑TGAGCCGCCAGCACCGACCGGGCTGCAGAAACTGGGTGAGGAAAAACAC‑GGTCAGG‑GGGTTTCTGTCGGTAAAAATGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGa                                               <  2:163428/236‑1 (MQ=255)                                                                                                                                                                                               tGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGT‑TGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACAC‑GGTCAGG‑TGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGcc                                           >  1:86622/1‑251 (MQ=255)                                                                                                                                                                                                    aaCGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGTTGT‑TGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACAC‑GGTCAGG‑TGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCAt                                      <  1:46538/251‑1 (MQ=255)                                                                                                                                                                                                                                 cGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGT‑TGCGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACAC‑GGTCAGG‑TGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAac          >  1:25325/1‑250 (MQ=255)                                                                                                                                                                                                                                      gTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGT‑TGAGCCGCCAGCACCGACCGtgctg                                                                                                                                                                        >  2:230640/1‑89 (MQ=255)                                                                                                                                                                                                                                      gTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGT‑TGAGCCGCCAGCACCGACCGtgctg                                                                                                                                                                        <  1:230640/89‑1 (MQ=255)                                                                                                                                                                                                                                        ccGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGT‑TGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACAC‑GGTCAGG‑TGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGt  >  1:61734/1‑251 (MQ=255)                                                                                                                                                                                                                                        ccGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGT‑TGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACAC‑GGTCAGG‑TGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGt  >  1:33991/1‑251 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                   CTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAACTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCATGAAGAAACTTTCGGTCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAATATCGGTCGTGTTATCGCTCGTCCGTTTATCGGCGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGT‑TGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACAC‑GGCCAGG‑TGGTTTCTGTCGGTAAAATTGCGGACATCTACGCCAACTGCGGTATCACCAAAAAAGTGAAAGCGACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGT  >  NC_000913/4620014‑4620495

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑