Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,619,454	A→G	N409S (AAC→AGC)	deoA →	thymidine phosphorylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,619,454	0	A	G	93.0%	98.2 / ‑3.0	44	N409S (AAC→AGC)	deoA	thymidine phosphorylase
Reads supporting (aligned to +/- strand):  ref base A (2/1);  new base G (13/27);  total (15/29)
Fisher's exact test for biased strand distribution p-value = 2.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.69e-01

TTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGACGCCGTCAGGCATCTGACACCATCGATTACAGCGTCGGCTTTACTGATATGGCGCGTCTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCG‑AAAGACGAAAACAACTGGCAGGAAGCGGCGAAAGCGGTGA‑AAGCGGCAATTAAACTTGCCGATAAAG‑CACCGGAAAGCACA‑CCAACTGTCTATCGCCGTATCAGCGAATAACGGTATACTGATCTGATCATTTAAATTTGAAGCACTGAGTACGGAGAAC‑ATATGAAACGTGCATTTATTATGGTGCTGGACTCATTCGGCATCGGCGCTACAGAAGATGCAGAACGCTTTGGTGACGTCGGGGCTGACA  >  NC_000913/4619208‑4619690                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                 gtGAGAACTACGCGAAGTATCGGCCGACAGCGATGATGACGAAAGCAGTATATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGACAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCTTTACTGATATGGCGCGTTTGGGCGACCAGGTAGACGGGCAGCGTCCGCTGGCGGTTATCCACGCT‑AAAGACGAAAACAGCTgg                                                                                                                                                                                                                                              <  2:434412/250‑1 (MQ=255)      aaCTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCTTTACTGATATGGCGCGTTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCG‑AAAGACGAAAACAGCTGGCAgg                                                                                                                                                                                                                                          >  1:214521/1‑250 (MQ=255)        cTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCTTTACTGATATGGCGCGTTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGGTTTTCCCCCCG‑AAAGACGAAAAAAGCTGGCAGGa                                                                                                                                                                                                                                         >  1:186712/1‑249 (MQ=255)          aCGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCTTTACTGATATGGCGCGTTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCG‑AAAGACGAAAACAGCTGGCAGGAAg                                                                                                                                                                                                                                       <  1:495334/249‑1 (MQ=255)          aCGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGGCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCGTTACTGATATGGCGCGTTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCG‑AAAGACGAAAACAGCTGGCAGGAAgcg                                                                                                                                                                                                                                     <  2:82640/251‑1 (MQ=255)                 gtttcgtcctgcagtgttGCTGACGAAAGCAGTCTAGGCTTATGCAGAATGTTGTGGCAGTGAAATGGAGACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGGGGCGGGCGTCGTCAGGCATCTTCCTCCAGTGATTTCAGCTGCTGCTTTACTGATATGGCGCGTTTGGGCGACCAGGTAGACGGTCAGCGGCCGCTGGCGGATATCCACGCG‑AAAGACTAAAACAGCTGGCAGGAAgcggcgcaa                                                                                                                                                                                                                               <  2:441375/233‑4 (MQ=255)                       gCCGACAGCGATGCGGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCTTTACTGATATGGCGCGTTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCG‑AAAGACGAAAACAGCTGGCAGGAAGCGGCGAAAGCGGt                                                                                                                                                                                                                          <  1:350867/249‑1 (MQ=255)                               agaggCTGACGAAAGCAGTTTCTGCTGAGACCGAAGGTTGTGGCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATGGATTACAGCGTCGGCTTTACTGAGATGGCGCGTTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCG‑AAAGAAGAAAACAGCTGGCAGGAAGCGGCGAAAGCGGCGA‑CAGCGGCa                                                                                                                                                                                                               <  2:97849/247‑1 (MQ=255)                                  tGCTGAAGAAAGCAGTCTATGCTGAAACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGAAGTGGTTGCACTGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCTTTACTGATATGGCGCGGTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGGTTGACCACGCGGAAAG‑CGAAAACAGCTGGGAGGGAGCGGCGGAGACGGGGA‑AAGCAGCAAtt                                                                                                                                                                                                            >  1:181723/1‑251 (MQ=255)                                       aCGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCTTTACTGATATGGCGCGTTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCG‑AAAGACGAAAACAGCTGGCAGGAAGCGGCGAAAGCGGTGA‑AAGCGGCAATTAAACt                                                                                                                                                                                                       <  2:81775/251‑1 (MQ=255)                                                 tCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCTTTACTGATATGGCGCGTTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGGATATCCAAGCG‑ACAGCCCAAAACCACCGGCAGGCACCGGCGCAAGCGGGGAGAAG‑GGCAATTAAACTTTCCGAtca                                                                                                                                                                                              >  1:364682/1‑248 (MQ=255)                                                      gCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCTTTACTGATATGGCGCGTTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCG‑AAAGACGAAAACAGCTGGCAGGAAGCGGCGAAAGCGGTGA‑AAGCGGCAATTAAACTTGCCGATAAAG‑CAcc                                                                                                                                                                                       <  1:437241/251‑1 (MQ=255)                                                             ccGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGGCGGCTTTACTGATATGGCGCGTTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCG‑AAAGACGAAAACAGCTGGCAGGAAGCGGCGAAAGCGGTGA‑AAGCGGCAATTAAACTTGCCGATAAAG‑CACCGGAAAGc                                                                                                                                                                                <  1:502749/251‑1 (MQ=255)                                                                 agtttttGTCAGGGAAAAGGATACCCGCGCGCTGGGGAGGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCTTTACTGATATGGCGCGTTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCG‑AAAGACGAAAACAGCTGGCAGGAAGCGGCGAAAGCGGTGA‑AAGCGGCAATTAAACTTGCCGATAAAG‑CACCGGAAAGCaca‑c                                                                                                                                                                           <  2:366105/248‑1 (MQ=255)                                                                   ggtttGGCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCTTTACTGATATGGCGCGTTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCG‑AAAGACGAAAACAGCTGGCAGGAAGCGGCGAAAGCGGTGA‑AAGCGGCAATTAAACTTGCCGATAAAG‑CACCGGAAAGCACA‑CCa                                                                                                                                                                         <  1:496009/249‑1 (MQ=255)                                                                        gTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCTTTACTGATATGGCGCGTTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCG‑AAAGACGAAAACAGCTGGCAGGAAGCGGCGAAAGCGGTGA‑AAGCGGCAATTAAACTTGCCGATAAAG‑CACCGGAAAGCACA‑CCAACTg                                                                                                                                                                     <  1:199447/250‑1 (MQ=255)                                                                                aaTGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCTTTACTGATATGGCGCGTTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGGGTGTCCACCCG‑GAAGACGAAAAAAGCTGGGAGGAAAAGGCGAAAACGGGGA‑AAGGCGCAATTAAAATTTCCGATTAAA‑CACCGGaaaagacacaaaattgctattccc                                                                                                                                                             >  1:503623/1‑230 (MQ=255)                                                                                                     gATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCTTTACTGATATGGCGCGTTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCG‑AAAGACGAAAACAGCTGGCAGGAAGCGGCGAAAGCGGGGA‑AAGCGGCAAATAAACTTGCCGATAAAG‑CACCGGAAAGCACA‑CCAAATGTCTATCGCCGTATCAGCGAATAACggg                                                                                                                                          >  1:424322/1‑247 (MQ=255)                                                                                                                          cgtcggtcgtcGTCAGGCATCTGACACCATTGATTACAGCGTCGGCTTTACTGATATGGCGCGTTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCG‑AAAGACGAAAACAGCTGGCAGGAAGCGGCGAAAGCGGTGA‑AAGCGGCAATTAAACTTGCCGATAAAG‑CACCGGAAAGCACA‑CCAACTGTCTATCGCCGTATCAGCGAATAACGGTATACTGATCTGATCATTTAAAttt                                                                                                                  <  1:120460/241‑1 (MQ=255)                                                                                                                            gcgggcgtcGTCAGGCATCTGAAACCATTGATTACAGCGTCGGCTTTACTGATATGGCGCGTTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCG‑AAAGACGAAAACAGCTGGCAGGAAGCGGCGAAAGCGGTGA‑AAGCGGCAATTAAACTTGCCGATAAAG‑CACCGGAAAGCACT‑CCAACTGTCTATCGCCGTATCAGCGAATAACGGTATACTGATCTGATCATTTAAATTTGa                                                                                                                <  1:43828/243‑1 (MQ=255)                                                                                                                             gggtcgtcGTCAGGCATCTGACACCATTGATTACAGCGTCGGCGTTACTGTTATGGCGCGTTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCG‑AAAGACGAAAACAGCTGGCAGGAAGCGGCGAAAGCGGTGA‑AAGCGGCAATTAAACTTGCCGATAAAG‑CACCGGAAAGCACA‑CCAACTGTCTATCGTCGTATCAGCGAATAACGGTATACTGATCTGATCATTTAGATTTGaa                                                                                                               <  2:86294/244‑1 (MQ=255)                                                                                                                             cggtcgtcGTCAGGCATCTGACACCATTGATTACAGCGTCGGCTTTACTGATATGGCGCGTTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCG‑AAAGACGAAAACAGCTGGCAGGAAGCGGCGAAAGGGGGGA‑AAGAGGCAATTAAACCTGCCGATAAAA‑CACCGGAAAG‑ACAACCAACTGTCTATCGCCGCATCACAGAAACACGGGATACTGATCTGATCATTTAAAtttta                                                                                                                >  2:73279/8‑248 (MQ=255)                                                                                                                                 cgtcGTCAGGCATCTGACACCATTGATTACAGCGTCGGCTTTACTGATATGGCGCGTTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCG‑AAAGACGAAAACAGCTGGCAGGAAGCGGCGAAAGCGGTGA‑AAGCGGCAATTAAACTTGCCGATAAAG‑CACCGGAAAGCACA‑CCAACTGTCTATCGCCGTATCAGCGAATAACGGTATACTGATCTGATCATTTAAATTTGAAGCa                                                                                                            <  1:427915/247‑1 (MQ=255)                                                                                                                                 cgtcGTCAGGCAGCTGACACCATTGATTACAGCGTCGGCGTTACTGATAGGGCGCGTTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCG‑AAAGACGAAAACAGCTGGCAGGAAGCGGCGAAAGCGGTGA‑AAGCGGCAATTAAACTTGCCGATAAAG‑CACCGGAAAGCACT‑CCAACTGTCTATCGCCGTATCAGCGAATAACGGTATACTGATCTGATCATTTAAATTTGAAGCAc                                                                                                           <  2:205469/248‑1 (MQ=255)                                                                                                                                     gtcgggCACCTGACACCATTGATTACAGCGTCGGCTGTACTGAGATGGCGCGTTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCG‑AAAGACGAAAACAGCTGGCAGGAAGCGGCGAAAGCGGTGA‑AAGCGGCAATTAAACTTGCCGATAAAG‑CACCGGAAAGCACA‑CCAACTGTCTATCGCCGTATCAGCGAATAACGGTATACTGATCTGATCATTTAAATTTGAAGCCCTGAg                                                                                                       <  2:53276/247‑1 (MQ=255)                                                                                                                                                gACACCATTGATTACAGCGTCGGCTTTACTGATATGGCGCGTTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCG‑AAAGACGAAAACAGCTGGCAGGAAGCGGCGAAAGCGGTGA‑AAGCGGCAATTAAACTTGCCGATAAAG‑CACCGGAAAGCACA‑CCAACTGTCTATCGCCGTATCAGCGAATAACGGTATACTGATCTGATCATTTAAATTTGAAGCACTGAGTACGGAGAAC‑a                                                                                           <  1:479376/251‑1 (MQ=255)                                                                                                                                                   cccatttatgaCAGCGTCTGCTTTACTGATCTGGCGGTTTTGGGCGCCCAGGTAGACGGGCAGCGACCGCTGGCTGTTATCCACGCG‑AAAGACGAAAACTTCTGTCAGGAAGCGGCGAAAGAGGTGC‑AAGCGGCAATTAAACTTGCCGATAAAG‑CACCGGAAAGCACA‑CCAACTGTCTATCACCGTATCAGCGAATAACTGTATACTGATCTGATCATTTAAATGTGAAGCACTGCGTACGGAGCAC‑atat                                                                                        <  2:167956/241‑1 (MQ=255)                                                                                                                                                    ccATTGATTACAGCGTCGGCTTTACTGATATGGCGCGTTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGGTTATCCCAGCG‑AAAGACGAAAACAGCCGGCAGGAAGCGGCGGAAGCGGGGG‑ACGAGGCCATTAAACTTGCCGCTAAAA‑CACCCGGAAGCACA‑CCAACTGGCTATTGCCCTATCAGCGAAAAACGGGATACTGATCTGATTCTTTAAATTTTAAGCACTgtggaccgggaaaatatt                                                                                        >  2:175285/1‑234 (MQ=255)                                                                                                                                                           gtACAGCGTCGGTTTCACTGATATGGCGGGGTGGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGGTTAGCCACGCG‑AAAGACGAATACAGCTGGCAGGAAGCGGCGAAAGCGGTGA‑AAGCGGCAATTAAACTTGCCGATAAAG‑CAGCGGAAAGCACA‑CCAACTGTCTATCGTCGTATCAGCGAATAACGGTATTCTGATCTGATCATTTAAATTTGAAGCACTGAGGACGTAGAAC‑ATCTGAAACGTg                                                                                <  2:86449/250‑1 (MQ=255)                                                                                                                                                            tACAGCGTCGGCTTTACTGATATGGCGCGTTTGGGCGACCAGGTAGAAGGTCAGCGTCCGCTGGCGGTTTTCCAAGCG‑AAAAACGAAAACAACTGGCAGGAAGCGGGGGAAGCGGGGA‑AAGAGGCAATTAAACTTTCCAATAAAA‑GACCGGAAAACCCA‑CCAACAGTCTATCGCCGTATAAGCGAATAACGGGCTACTGATCTTATCATTTAAATTTGTAGAACTgcggacagacaacataagtaaagggc                                                                                >  1:373706/1‑226 (MQ=255)                                                                                                                                                                 ggTCGGCTTTACTGATATGGCGCGTTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCG‑AAAGACGAAAACAGCTGGCAGGAAGCGGCGAAAGCGGTGA‑AAGCGGCAATTAAACTTGCCGATAAAG‑CACCGGAAAGCACA‑CCAACTGTCTATCGCCGTATCAGCGAATAACGGTATACTGATCTGATCATTTAAATTTGAAGCACTGAGTACGGAGAAC‑ATATGAAACGTGCATtta                                                                          <  1:158341/250‑1 (MQ=255)                                                                                                                                                                        tttACTGCTATGGCGCGTTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGGCTATCCACCCG‑AAAGACGGACACAGCTCGCAGGAAGCGGCGCAAGCGGTGA‑CCGCGGCCATTAAACATTCCCATAAAGACCCCCGAA‑GCACC‑CCAACTGTCTATCCCCGTTTTAGCGAATAACGGGGAACTGATTCGGTCATTTAAATTTTAAGCACCGACGACGGGGAAC‑AAATGAAAAAGGCATTTTTTATggg                                                                   >  2:46747/1‑250 (MQ=255)                                                                                                                                                                          tACTGATATGGCGCGTTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCG‑AAAGACGAAAAAAGCTGGCAGGGAGCGGCGAAAGCGGTGA‑AAGCGGCAATTAAACTTGCCGATAAAG‑CACCGGAAAGCACA‑CCAACCGGCTATCGCCGTATCAGCGAATAACGGGATACTGATCTGATCATTTTAATTTGAAGCAATGCGGACGGGGAACCTTAT‑AAAAGTGCATTTTTTATGGTgg                                                                 >  1:381693/1‑250 (MQ=255)                                                                                                                                                                                        gtttGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCG‑AAAGACGAAAACCGCTGGCAGGGAGAGGCGAAAGAGGTGA‑AAAGGGCAATTAAAATTTCCGCTAAAG‑CACCGGAAAGCACA‑CCAAATGTCTATCTACGCATCCGCGAAAAACGGGATACTTATCTGATCCATTAAATTTGAAGGACTGAGTCCGGAGGAA‑ATCTGAAACGTGCATTTTTTATTGTGCTGGACTCATTCggg                                                   >  2:267797/4‑250 (MQ=255)                                                                                                                                                                                          cTGGGCGACCAGGTATACGGTCAGCGTCCGCTGGCGGTTAACCACGCG‑AAAGACGAAAACAACTGGCAGGAAGCGGCGAAAGCGGTGA‑AAGCGGCAATTa                                                                                                                                                                                                           <  1:245225/100‑1 (MQ=255)                                                                                                                                                                                                   cAGGTAGACGGTCAGCGTCCGCTGGCGGTAATCCACGCG‑CAAAACGAAAACCGCTGGCAGGAAGCGGGGGAAGCGGTGA‑AAACGGGCATTAAACATGCCGGTAAAG‑CACCGGAAAGCACA‑CCACCTGTATATAGCCGTATCAGCGAATAAAGGGATACTGATCTTAACCTTTAAATTTGAAGCACTGAGGACGGAGAAC‑AAAATAAAAGAGCATTTTTTATGGGTCTTGGCACATTCGGCATCggggcaac                                        >  2:489338/1‑245 (MQ=255)                                                                                                                                                                                                    aGGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCG‑AAAGACGAAAACAGCTGGCAGGAAGCGGCGAAAGCGGTGA‑AAGCGGCAATTAAACTTGCCGATAAAG‑CACCGGAAAGCACA‑CCAACTGTCTATCGCCGTATCAGCGAATAACGGTATACTGATCTGATCATTTAAATTTGAAGCACTGAGTACGGAGAAC‑ATATGAAACGTGCATTTATTATGGTGCTGGACTCATTCGGCATCGGCGCTACa                                       <  2:22137/251‑1 (MQ=255)                                                                                                                                                                                                      gTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCG‑AAAGACGAAAACAGCTGGCCGGAAGCGGCGAAAGCGGTGA‑AAGCGGCAATTAAACTTGCCGATAAAG‑CACCGGAAAGCACA‑CCAACTGTCTATCGCCGTATCAGCGAATAACGGTATACTGATCTGATCATTTAAATTTGAAGCACTGAGTACGGAGAAC‑ATATGAAACGTGCATTTTTTATGGTGCTGGACTCATTCGGCCTCGGCGCTaa                                        >  1:461938/1‑247 (MQ=255)                                                                                                                                                                                                        aGACGGTCAGCGTCCGCTGGCGGTTATCCACGCG‑AAAGACGAAAACAGCTGGCAGGAAGCGGCGAAAGCGGTGA‑AAGCGGCAATTAAACTTGCCGATAAAG‑CACCGGAAAGCACA‑CCAACTGTCTATCGCCGTATCAGCGAATAACGGTATACTGATCTGATCATTTAAATTTGAAGCACTGAGTACGGAGAAC‑ATATGAAACGTGCATTTATTATGGTGCTGGACTCATTCGGCATCGGCGCTACagaa                                    <  2:461938/250‑1 (MQ=255)                                                                                                                                                                                                             gTCAGCGTCCGCTGGGGGTTATCCACGCG‑AAAGACGAAAACAGCTGGCAGGAAGCGGCGAAAGCGGTGA‑AAGCGGCAATTAAACTTGCCGATAAAG‑CACCGGAAAGCACA‑CCAACTGTCTATCGCCGTATCAGCGAATAACGGTATACTGATCTGATCATTTAAATTTGAAGCACTGAGTACGGAGAAC‑ATATGAAACGTGCATTTATTATGGTGCTGGACTCATTCGGCATCGGCGCTACAGAAGATGCa                              <  2:424322/251‑1 (MQ=255)                                                                                                                                                                                                                               ttATCCACGCG‑AAAGACGAAAACAGCTGGCAGGCAGCGGCGAATGCGGTGA‑AAGCGGCAATTAAACTTGCCGATAAAG‑CACCGGAAAGCACA‑CCAACTGTCTATCGCCGTATCAGCGAATAACGGTATACTGCTCTGATCATTTCAATTTGAAGCACTGAGTCCGGAGAAC‑CTATGAAACGTGCATTTATTATGGTGCTGGACTCATTCGGCATCGGCGCTACAGACGATGCAGAACCCTTTGGTAACGTc            >  2:258111/1‑251 (MQ=255)                                                                                                                                                                                                                                        cg‑AAAGACGAAAACAGCTGGCAGGAAGCGGCGAAAGCGGTGA‑AAGCGGCAATTAAACTTGCCGATAAAG‑CACCGGAAAGCACA‑CCAACTGTCTATCGCCGTATCAGCGAATAACGGTATACTGATCTGATCATTTAAATTTGAAGCACTGAGTACGGAGAAC‑ATATGAAACGTGCATTTATTATGGTGCTGGACTCATTCGGCATCGGCGCTACAGAAGATGCAGAACGCTTTGGTGACGTCGGGGCTGac   <  2:476055/251‑1 (MQ=255)                                                                                                                                                                                                                                        cg‑AAAGACGAAAACAGCTGGCAGGAAGCGGCGAAAGCGGTGA‑AAGCGGCAATTAAACTTGCCGATAAAG‑CACCGGAAAGCACA‑CCAACTGTCTATCGCCGTATCAGCGAATAACGGTATACTGATCTGATCATTTAAATTTGAAGCACTGAGTACGGAGAAC‑ATATGAAACGTGCATTTATTATGGTGCTGGACTCATTCGGCATCGGCGCTACAGAAGATGCAGAACGCTTTGGTGACGTCGGGGCTGac   <  1:490118/251‑1 (MQ=255)                                                                                                                                                                                                                                         g‑AAAGACTAAATCAGCTGGCAGGAAGCGGCGAAAGCGGTGA‑AAGCGGCAATTAAACTTGCCTATAAAG‑CACCGGAAAGCACT‑CCAACTGGCTATCGCCGTAGCAGCGAATAACGGTATACTGATCTGATCATTTAAATTTGAAGGACTGAGTACGGAGAAT‑ATATTAAACGTTCATTGATTATGGTGCTGGACTCGTTCGGCATCGGCGCTACAGAAGATGCAGAACGCTTTGGTGACGTCGGGGCTGaca  <  1:60556/251‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                 TTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGACGCCGTCAGGCATCTGACACCATCGATTACAGCGTCGGCTTTACTGATATGGCGCGTCTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCG‑AAAGACGAAAACAACTGGCAGGAAGCGGCGAAAGCGGTGA‑AAGCGGCAATTAAACTTGCCGATAAAG‑CACCGGAAAGCACA‑CCAACTGTCTATCGCCGTATCAGCGAATAACGGTATACTGATCTGATCATTTAAATTTGAAGCACTGAGTACGGAGAAC‑ATATGAAACGTGCATTTATTATGGTGCTGGACTCATTCGGCATCGGCGCTACAGAAGATGCAGAACGCTTTGGTGACGTCGGGGCTGACA  >  NC_000913/4619208‑4619690

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑