Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,619,338	C→T	R370R (CGC→CGT)	deoA →	thymidine phosphorylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,619,338	0	C	T	97.1%	96.9 / NA	36	R370R (CGC→CGT)	deoA	thymidine phosphorylase
Reads supporting (aligned to +/- strand):  ref base C (1/0);  major base T (13/20);  minor base G (1/0);  total (16/20)
Fisher's exact test for biased strand distribution p-value = 4.12e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAGGCGGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCGACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGACGCCGTCAGGCATCTGACACCATCGATTACAGCGTCGGCTTTACTGATATGGCGCGTCTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCG‑AAAGACGAAAACAACTGGCAGGAAGCGGCGAAAGCGGTGA‑AAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATAACGGT  >  NC_000913/4619093‑4619555                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                            cTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGgtcgtcgtc                                                                                                                                                                                                                         >  1:205469/1‑250 (MQ=255)      gAAAGATGACGCCGAAGCGCGCGCGAAATTGCAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGGCTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCAt                                                                                                                                                                                                                   >  2:379803/1‑251 (MQ=255)              aCGCCGAAGCGCGCGCGAACTTGCAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGCCTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGCCGCAAGCCGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGCTGGCGGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGGATCTGACAcc                                                                                                                                                                                                           >  2:496009/1‑251 (MQ=255)                             cgAAATTGCAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTc                                                                                                                                                                                            >  2:479376/1‑251 (MQ=255)                              gAAATTGCAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCg                                                                                                                                                                                           <  1:228064/251‑1 (MQ=255)                                 aTTGCAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAAACGACTGCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCt                                                                                                                                                                                        <  2:372800/251‑1 (MQ=255)                                      gcgcagTGCTGGACAACGGTCAAGCGGCGGAAGTCTTTGGTCGTATGGTAGCGGAACAACAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCAGACAGCGCTGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGTTCCCCGCGCGCTGGGGGTGGCAGTGGTTGCAATGGGCGGCGGTCGTCGGCAGGCCTCTGACCCCCTTGATTACaacggagcctttctt                                                                                                                                                                                   >  2:103888/6‑237 (MQ=255)                                                     aCGGAAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAACAGGCACAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGATGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGTAATGGATACCCGCCCGCTGGGGATGGGAGTGTTTGCAATGGGGGGCGGTCGGCGTCGAGCATCTGACACCATTGATTACAGCGACGGCTTTACTGATATGGCGCGtttg                                                                                                                                                                    >  2:355067/1‑248 (MQ=255)                                                         tAAAGCGGCCGAAGGCGTTGGTCGAATGGGAACGGCCCAAAAAGGCCCCACCGACTTCGTTGAGAACGCAGCGAAGTATCAGCAGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACACGCGCGATGGGGATGGCAGTGGTTGCCATGGGCGGCGGTCTACTTCCGGCATCTGCCACCATTGTTTACAGCGTCGGCGTTACTGCTATGGCGCGTTTggggg                                                                                                                                                                >  2:492891/1‑249 (MQ=255)                                                                          ttGGCCGTCTGGTACCGGTACAAAAGTGCCCGACCGACTTCATGGAGACCTCCGCGAATCACCTGACGTCCGCCATGCTGACGAACGCAGTCTATGCTCATACCGAAGGTTTTGTCAGGGAATCGGTTACCCGCGCGCTGGGGATGGCAGTGGTTGCAAGGGGCGGCGGTCTTCGTCAGGCATCTGACGCCATTGATTACAGCGTCGGATTTACTGATATGGCGCGTTTGGGCGagcattgagccggtca                                                                                                                                                <  2:62730/250‑16 (MQ=255)                                                                           tGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCTTTACTGATATGGCGCGTTTGGGCGACCAGGTAGACGGTCAg                                                                                                                                               <  1:274465/250‑1 (MQ=255)                                                                                       aGCGGCACAAAAAGGCCCAACCGGCTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCTTTACTGATATGGCGCGTTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCg                                                                                                                                   <  1:379803/250‑1 (MQ=255)                                                                                       aGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGTCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCTTTACTGATATGGCGCGTTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCg                                                                                                                                   <  1:77781/250‑1 (MQ=255)                                                                                          ggCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCTTTACTGATATGGCGCGTTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGGTTa                                                                                                                               <  1:19236/251‑1 (MQ=255)                                                                                              caAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACACGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGGCGCCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCATTACTGATATGGCGCGTTTGGGCGGCGAGGTAGACGGGCAGCGTCCGCTGGCGGTTaaccc                                                                                                                           >  2:193677/1‑247 (MQ=255)                                                                                                   aGGCCCAACCGACTTCGTTGCGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGAAACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGGCAGGCATCTGACACCATTGATTACAGCGGCGGCTTTACTGATATGGCGCGTTTGGGCGACCAGGTAGACGGGCAGCGTGCGCTGGGGGGTATctcaccgg                                                                                                                      >  2:47189/1‑244 (MQ=255)                                                                                                    ggCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCTTTACTGATATGGCGCGTTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGGTTATCCAcgcgc                                                                                                                      >  1:476055/1‑249 (MQ=255)                                                                                                    ggCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCTTTACTGATATGGCGCGTTTGGGCGACCAGGGAGACGGTCAGCGTCCGCTGGCGGGTATCCAcgcgg                                                                                                                      >  2:437241/1‑249 (MQ=255)                                                                                                        caaccgacTTCGTTGAGAACTACGCGAATTATCTGCCGACAGCGATGCGTACGAAAGCAGTCTATGCTGATACCGAATGTTTGGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCTTTTCTGATATGGCGCGTTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCG‑AAAGAc                                                                                                                <  2:406831/249‑1 (MQ=255)                                                                                                                    gtGAGAACTACGCGAAGTATCGGCCGACAGCGATGATGACGAAAGCAGTATATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGACAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCTTTACTGATATGGCGCGTTTGGGCGACCAGGTAGACGGGCAGCGTCCGCTGGCGGTTATCCACGCT‑AAAGACGAAAACAGCTgg                                                                                                    <  2:434412/250‑1 (MQ=255)                                                                                                                         aaCTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCTTTACTGATATGGCGCGTTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCG‑AAAGACGAAAACAGCTGGCAgg                                                                                                >  1:214521/1‑250 (MQ=255)                                                                                                                           cTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCTTTACTGATATGGCGCGTTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGGTTTTCCCCCCG‑AAAGACGAAAAAAGCTGGCAGGa                                                                                               >  1:186712/1‑249 (MQ=255)                                                                                                                             aCGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCTTTACTGATATGGCGCGTTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCG‑AAAGACGAAAACAGCTGGCAGGAAg                                                                                             <  1:495334/249‑1 (MQ=255)                                                                                                                             aCGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGGCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCGTTACTGATATGGCGCGTTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCG‑AAAGACGAAAACAGCTGGCAGGAAgcg                                                                                           <  2:82640/251‑1 (MQ=255)                                                                                                                                    gtttcgtcctgcagtgttGCTGACGAAAGCAGTCTAGGCTTATGCAGAATGTTGTGGCAGTGAAATGGAGACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGGGGCGGGCGTCGTCAGGCATCTTCCTCCAGTGATTTCAGCTGCTGCTTTACTGATATGGCGCGTTTGGGCGACCAGGTAGACGGTCAGCGGCCGCTGGCGGATATCCACGCG‑AAAGACTAAAACAGCTGGCAGGAAgcggcgcaa                                                                                     <  2:441375/233‑4 (MQ=255)                                                                                                                                          gCCGACAGCGATGCGGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCTTTACTGATATGGCGCGTTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCG‑AAAGACGAAAACAGCTGGCAGGAAGCGGCGAAAGCGGt                                                                                <  1:350867/249‑1 (MQ=255)                                                                                                                                                  agaggCTGACGAAAGCAGTTTCTGCTGAGACCGAAGGTTGTGGCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATGGATTACAGCGTCGGCTTTACTGAGATGGCGCGTTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCG‑AAAGAAGAAAACAGCTGGCAGGAAGCGGCGAAAGCGGCGA‑CAGCGGCa                                                                     <  2:97849/247‑1 (MQ=255)                                                                                                                                                     tGCTGAAGAAAGCAGTCTATGCTGAAACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGAAGTGGTTGCACTGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCTTTACTGATATGGCGCGGTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGGTTGACCACGCGGAAAG‑CGAAAACAGCTGGGAGGGAGCGGCGGAGACGGGGA‑AAGCAGCAAtt                                                                  >  1:181723/1‑251 (MQ=255)                                                                                                                                                          aCGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCTTTACTGATATGGCGCGTTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCG‑AAAGACGAAAACAGCTGGCAGGAAGCGGCGAAAGCGGTGA‑AAGCGGCAATTAAACt                                                             <  2:81775/251‑1 (MQ=255)                                                                                                                                                                    tCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCTTTACTGATATGGCGCGTTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGGATATCCAAGCG‑ACAGCCCAAAACCACCGGCAGGCACCGGCGCAAGCGGGGAGAAG‑GGCAATTAAACTTTCCGAtca                                                    >  1:364682/1‑248 (MQ=255)                                                                                                                                                                         gCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCTTTACTGATATGGCGCGTTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCG‑AAAGACGAAAACAGCTGGCAGGAAGCGGCGAAAGCGGTGA‑AAGCGGCAATTAAACTTGCCGATAAAGCAcc                                              <  1:437241/251‑1 (MQ=255)                                                                                                                                                                                ccGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGGCGGCTTTACTGATATGGCGCGTTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCG‑AAAGACGAAAACAGCTGGCAGGAAGCGGCGAAAGCGGTGA‑AAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGc                                       <  1:502749/251‑1 (MQ=255)                                                                                                                                                                                    agtttttGTCAGGGAAAAGGATACCCGCGCGCTGGGGAGGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCTTTACTGATATGGCGCGTTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCG‑AAAGACGAAAACAGCTGGCAGGAAGCGGCGAAAGCGGTGA‑AAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCacac                                   <  2:366105/248‑1 (MQ=255)                                                                                                                                                                                      ggtttGGCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCTTTACTGATATGGCGCGTTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCG‑AAAGACGAAAACAGCTGGCAGGAAGCGGCGAAAGCGGTGA‑AAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCa                                 <  1:496009/249‑1 (MQ=255)                                                                                                                                                                                           gTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCTTTACTGATATGGCGCGTTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCG‑AAAGACGAAAACAGCTGGCAGGAAGCGGCGAAAGCGGTGA‑AAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTg                             <  1:199447/250‑1 (MQ=255)                                                                                                                                                                                                   aaTGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCTTTACTGATATGGCGCGTTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGGGTGTCCACCCG‑GAAGACGAAAAAAGCTGGGAGGAAAAGGCGAAAACGGGGA‑AAGGCGCAATTAAAATTTCCGATTAAACACCGGaaaagacacaaaattgctattccc                    >  1:503623/1‑230 (MQ=255)                                                                                                                                                                                                                        gATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCTTTACTGATATGGCGCGTTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCG‑AAAGACGAAAACAGCTGGCAGGAAGCGGCGAAAGCGGGGA‑AAGCGGCAAATAAACTTGCCGATAAAGCACCGGAAAGCACACCAAATGTCTATCGCCGTATCAGCGAATAACggg  >  1:424322/1‑247 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                            CTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAGGCGGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCGACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGACGCCGTCAGGCATCTGACACCATCGATTACAGCGTCGGCTTTACTGATATGGCGCGTCTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCG‑AAAGACGAAAACAACTGGCAGGAAGCGGCGAAAGCGGTGA‑AAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATAACGGT  >  NC_000913/4619093‑4619555

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑