Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,822	T→C	D363D (GAT→GAC)	thrC →	threonine synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,822	0	T	C	97.1%	99.0 / ‑5.5	35	D363D (GAT→GAC)	thrC	threonine synthase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (14/20);  minor base A (1/0);  total (15/20)
Fisher's exact test for biased strand distribution p-value = 4.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.73e-01

TTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGGGCTACACTTCGGAGCCGCACGCTGCCGTAGCTTATCGTGCGCTGCGTGATCA‑GTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTT‑‑AAAGAGAGCGTGGAAGCGATTCTCGGTGAAAC‑GTTGGATC‑TGCCAAAAGAGCTGGCAGAACGTGCTGATTTACCCTTGCTTTCACATAATCTGCCCGCCGATTTTGCTGCGTTGCGTAAATTGATGATGAATCATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTA  >  NC_000913/4577‑5059                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                   ttCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGGGCTACACCTCGGAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCA‑Gtt                                                                                                                                                                                                                                              >  1:350103/1‑251 (MQ=255)     tGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGGGCTACACCTCGGAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCA‑GTTGAAt                                                                                                                                                                                                                                          >  1:437112/1‑251 (MQ=255)                        cccaaaaGCGACTCAGGGAACGTTACCCAAGGGGTGGGACGTGAGTCAGCCGAACAACTGGCCGGGTGGGAAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGGGCTACACCTCGGAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCA‑GTTGAATCCAGGCGAATATGGCTTGt                                                                                                                                                                                                                       <  2:484471/247‑1 (MQ=255)                          ccAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGCACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGGGCTACAACTCGGAGCAGCACGCTGCCGTAGCGTATCGTGCGCTGCG‑GAACAAGTTGAATCCAGGCGAATATGGCTTGTTc                                                                                                                                                                                                                     >  1:381167/1‑251 (MQ=255)                                                    aaCGCGATGGACGTGATTCAGCCGCACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGCCAATGCGTGAGTTAAAAGAACTGGGCTACACCTCGGAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCA‑GTTGAATCCCGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGGAAtt                                                                                                                                                                                           >  1:450085/1‑251 (MQ=255)                                                                   aGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGGGCTACACCTCGGAGCCGCACGCTGCCGTAGCGAATCGTGCGCTGCGTGACCA‑GTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCCCGCCTCCGGCGAAATTT‑‑AAAGAGAGCggggg                                                                                                                                                                          >  2:360378/1‑247 (MQ=255)                                                                    gTCAGCCGAACAACGTGCCGGGGGTGGAAGGGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGGGCTACACCTCGGAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCA‑GTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTT‑‑AAAGAGAGCGTGGaa                                                                                                                                                                         <  2:250062/251‑1 (MQ=255)                                                                     tCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAACTCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAAACAAGCAACAGACAATGAGGGAGTTAAAAGAACTGGGCTACACCTCGGAGCCGCACGCAGCCGTAGCGAATCGGGCGCTGCGTGACCA‑GTTGAATCCAGGCGAATGTGGATTGTTCCTCAGCACCTCACATCCGGCGACATTTCAAAagagagagaa                                                                                                                                                                            >  2:78303/1‑245 (MQ=255)                                                                       cgccgaccactgtgCCGCGTGTGTAAGAGTGGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGGGCTACACCTCGGAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCA‑GTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATGT‑‑AAAGAGAGCGTGGAAGCg                                                                                                                                                                      <  2:441408/238‑1 (MQ=255)                                                                             acaccTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGGGCTACACCTCGGAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCA‑GTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTT‑‑AAAGAGAGCGTGGAAGCGATtctc                                                                                                                                                                <  1:469407/247‑1 (MQ=255)                                                                             acaatgggccgggggtgGAAGTGTGGTTCCGCCGCAAAAACTGTCTACTGAAAGAGCTGGGTTATGCAGCCGTTGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGGGCTATACCTCGGAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCA‑GTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTT‑‑AAAGAGAGCGTGGAAGCGATtctc                                                                                                                                                                <  2:484006/237‑1 (MQ=255)                                                                                    gCCGCGTGGGAAAGGGTGGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCGGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGGGCTACACCTCGGAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCA‑GTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTT‑‑AAAGAGAGCGTGGAAGCGATTCTCGGTGaaa                                                                                                                                                         <  2:6450/251‑1 (MQ=255)                                                                                     ccGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGGGCTACACCTCGGAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCA‑GTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTT‑‑AAAGAGAGCGTGGAAGCGATTCTCGGTGAAAc                                                                                                                                                        <  1:182889/251‑1 (MQ=255)                                                                                                        tCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGGGCTACACCTCGGAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCA‑GTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTT‑‑AAAGAGAGCGTGGAAGCGATTCTCGGTGAAAC‑GTTGGATC‑TGCCAAAag                                                                                                                                     >  1:83681/1‑249 (MQ=255)                                                                                                                       ggaaaCTGAAAGAGCGTGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGGTAAAAGAACTGGGCGACACCTCGGGGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCA‑GTTGAATCCAGGCGAATATGGCGTGTTCCTCGGCACCGCGCATCCGGCGAAATTG‑‑AAAGAGAGCGTGGAAGCGATTCTCGGTGAAAC‑GTTGGATC‑TGCCAAAAGAGCTGGCAGAACGTGc                                                                                                                     <  2:255114/247‑1 (MQ=255)                                                                                                                                 agagCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGGGCTACACCTCGGAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCA‑GTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTT‑‑AAAGAGAGCGTGGAAGCGATTCTCGGTGAAAC‑GTTGGATC‑TGCCAAAAGAGCTGGCAGAACGGGCTGATTTACCCt                                                                                                          >  1:267811/1‑251 (MQ=255)                                                                                                                                                      ggATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGGGCTACACCTCGGAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCA‑GTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTT‑‑AAAGAGAGCGTGGAAGCGATTCTCGGTGAAAC‑GTTGGATC‑TGCCAAAAGAGCTGGCAGAACGTGCTGATTTACCCTTGCTTTCACATAATCTGccc                                                                                      <  1:75739/250‑1 (MQ=255)                                                                                                                                                       gatgatGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGGGCTACCCCTCGGAGCCGCACGCTGCCGTAGCGCATAGTGCGCTGCGTGCCCA‑GTTGAATCCAGCCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGACGAAATTT‑‑AAAGAAAGCGTGGAAGCGATTCTCGGTGAAAC‑GTTGGATC‑TGCCAAAAGAGCTGGCAGACCGTGCTGCTTTAACCCTGCTTTCACATAATCTGCccgc                                                                                    >  1:484427/1‑251 (MQ=255)                                                                                                                                                                                 gCGTGAGTTAAAAGAACTGGGCTACACCTCGGAGCCGCCCGCTGCCGTAGCGTATCGTGCGCTGCGTGACCA‑GTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTT‑‑AAAGAGAGCGTGGAAGCGATTCTCGGTGAAAC‑GTTGGATC‑TGCCAAAAGAGCTGGCAGAACGTGCTGATTTACCCTTGCTTTCACATAATCTGCCCGCCGATTTTGCTGCGTTGCGTAAATtg                                                           <  1:344557/250‑1 (MQ=255)                                                                                                                                                                                    ggattaaaaaaaACTGGCCTACTCCTCGGGGCCGCACGCGGCCGTAGCGTTTCGTGCGCTGCGTGACCA‑GTTGATTCCAGGCGAATATGGCTTGTTCCTCGGCCCCGCGCATCCGGCGAAATTT‑‑AAAGAGAGCGTGGAAGCGTTTCTCGGTGAACC‑GTTGGATC‑TGCCAAAAGAGCTGGCAGAACGTGCTGATTTACCCTTGCTTTCACATAATCTGCCCGCCGATTTTGCTGCGTTGCGTAAATtgatga                                                       <  1:367367/245‑1 (MQ=255)                                                                                                                                                                                         aaaaagacgtgggctccccccgggAGCCGCCCGCCGCCTTAGCGTATCGTGCGCTGCGGGACCA‑GTTAAATCCAGGAAAATAGGGCTTTTTCCCCGGCACCGCGCACCCGGCGAAATTA‑‑AAAGAGAGGGTGGAAGCGATTCTCGG‑GAAACCGTTGGATC‑TGCCAAAAGGGCTGGCAGAACGTGCTGATTTACCCTTGTTTTAACAGAATCTGCCCGCCGATTTTGCTGCGTTGCGTAAATTGATGATGaa                                                   <  2:437112/228‑1 (MQ=255)                                                                                                                                                                                             aGAACTGGGCTACACCTCGGAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCA‑GTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTT‑‑AAAGAGAGCGTGGAAGCGATTCTCGGTGAAAC‑GTTGGATC‑TGCCAAAAGAGCTGGCAGAACGTGCTGATTTACCCTTGCTTTCACATAATCTGCCCGCCGATTTTGCTGCGTTGCGTAAATTGATGATGAatcatc                                              <  1:150570/251‑1 (MQ=255)                                                                                                                                                                                                  ggggCTACCCCTCGGAGCCGCACGCTGCGGTAGCGTATCGTGCGCTGCGTGACCA‑GTTGAATCCAGGCGAATATGGCTTGTTCCCCGGCACCGCGCATCCGGCGAAATTT‑‑AAAGAGAGCGTGGAAGCGATTCTCGGTGAAAC‑GTTGGATC‑TGCCAAAAGAGCTGGCAGAACGTGCTGATTTCCCCTTGCTTTCACATAATCTGCCCGCCGATTTTGCTGCGTTGCGTAAATTGATGATGAATCATCAGTaa                                         <  1:492819/250‑1 (MQ=255)                                                                                                                                                                                                   gggctccccctCGGAGCCGCCCGCTGCCGTAGCGTATCGTGCGCTGCGTGACCA‑GTTGAATCCAGGCGAATATGTCTTGTTCCTCGGCACCGCCCATCCGGCGAAATTT‑‑AAAGAGAGCGTGGAAGCGATTCTCGGTGAAAC‑GTTGGATC‑TGCCAAAAGAGCTGGCAGAACGTGCTGATTTCCCCTTGCTTTCACATAATCTGCCCGCCGATTTTGCTGCGTTGCGTGAATTGATGATGAATCATCAGTaaa                                        <  1:63385/241‑1 (MQ=255)                                                                                                                                                                                                      cTACACCTCGGAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCA‑GTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTT‑‑AAAGAGAGCGTGGAAGCGATTCTCGGTGAAAC‑GTTGGATC‑TGCCAAAAGAGCTGGCAGAACGTGCTGATTTACCCTTGCTTTCACATAATCTGCCCGCCGATTTTGCTGCGTTGCGTAAATTGATGATGAATCATCAGTAAAATc                                     >  1:79790/1‑251 (MQ=255)                                                                                                                                                                                                        acacCTCGGGGCCGGACGATGCAGTAGCGGATCGTGCGCTGAGTGACCC‑GTTGAATCCAGGCGAATATGGCTTGGTCCTCGGCACCGCGCATCCGGCGAAATTT‑‑AAAGAGAGCGTGGAAGCGAGTCTCGGTGAAAC‑ATTGGATC‑TGCCAAAAGAGCTGGCAGAACGTGCTCATTTACCCTTGCTTTCGCATAGTCTGCCCGCCGGTTTTGCTGCGTTGTGGAAATTGCTTAAGAATCATCAGTAAActct                                    >  2:90595/1‑250 (MQ=255)                                                                                                                                                                                                         cacctcggaaacGCACGCTGCCGAAGCGTATCGTGCGCTGCATCGCCA‑GTTGAATCCAGGCGAATATGGATTGTTCCTCGGCACCGCGCATCCGGCGAAATTT‑‑CAAGCGAGAGTGAAAGCGCTTCTCGGTGAAAC‑GGTGGATCATACCTAA‑GAACCTGCAGAACGTGCTGATTTACCCTTGCTTTCACATCATCTGCCCGCCGCTTTTTCTGCGTTTAGAAACTTGATGGTGAATCATCAGTaaacactat                                  >  2:459910/12‑245 (MQ=255)                                                                                                                                                                                                                gAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCA‑GTTGAATACAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTT‑‑AAAGAGAGCGTGGAAGCGATTCTCGGTGCAAC‑GTTGGATC‑TGCCAAAAGAGCTGGCAGAACGTGCTGATTTACCCTTGCTTTCACATAATCTGCCCGCCGATTTTGCTGCGTTGCGTAACTTGATGGTGAATCATCAGTAAAATCTATTCATTAt                           >  1:447430/1‑251 (MQ=255)                                                                                                                                                                                                                  gCCGCACGCTGCTGTAGCGTATCGTGTGCTGCGTGACCA‑GTTGAATCCAGGAGAATAGGGCTTGTACCTCGGCACCGCGCATCCGGCGAAATTT‑‑AAAGAGAGCGTGGAAGCGATTCTCGGTGAATC‑GTTGGATC‑TGCCAAAAGAGCTGGCAGACCGTGCTGATTTCCCCTTGCTTTCACATAATCTGCCCGCCGATTTTGCTGCGTTGCGTAAATTGATGATGAATCATCAGTAAAATCTATTCATTAtct                         <  1:7692/251‑1 (MQ=255)                                                                                                                                                                                                                     ccgctcTGCCGGAGCGTATCGTGCGCTGCGTAACCT‑GTTGAATCAAGGCGAATATGGCTTGTTCCTCGGCCCCGCGCATCGGGCGAAATTT‑‑AAAGAGGGCTTGGAAGCGATTCTCGGTGAAAC‑TTTGGATC‑TGCCAAAAGAGCTGGCAGAACGTGCTGTTTTACCCTTGCTTTCACATAATCTGCCCGCCGATTTTGCTGCGTTGCGTAAATTGATGATGAATCATCAGTAAAATCTATTCATTATCTCaa                      <  1:138244/246‑1 (MQ=255)                                                                                                                                                                                                                      caggCTGCCGTAGCGTATCGTGCGCTGCGTGACCA‑GTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTT‑‑AAAGAGAGCGTGGAATCGATTCTCGGTGAAAC‑GTTGGATC‑TGCCAAAAGAGCGGGCAGAACGTGCTGATTTCCCCTTGCTTTCACATAATCTGCCCGCCGATTTTGCTGCGTTGCGTAAATTGATGATGAATCATCAGTAAAATCTATGCATTATCTCAAt                     <  1:114087/248‑1 (MQ=255)                                                                                                                                                                                                                         gCTGCCGTAGCGTATCGTGCGCTGCGTGACCA‑GTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTT‑‑AAAGAGAGCGTGGAAGCGATTCTCGGTGAAAC‑GTTGGATC‑TGCCAAAAGAGCTGGCAGAACGTGCTGATTTACCCTTGCTTTCACATAATCTGCCCGCCGATTTTGCTGCGTTGCGTAAATTGATGATGAATCATCAGTAAAATCTATTCATTATCTCAATc                    >  1:389692/1‑249 (MQ=255)                                                                                                                                                                                                                            gCCGTAGCGTATCGGGCGCTGCGTGACCA‑GTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTT‑‑AAAGAGAGCGTGGAAGCGATTCTCGGTGAAAC‑GTTGGATC‑TGCCAAAAGAGCTGGCAGAACGTGCTGATTTACCCTTGCTTTCACATAATCTGCCCGCCGATTTTGCTGCGTTGCGTAAATTGATGATGAATCATCAGTAAAATCTATTCATTATCTCAATCAgg                 <  1:360378/249‑1 (MQ=255)                                                                                                                                                                                                                                      ttggggctcTGCGGGACCA‑GTTAAATCCGGGGGAATAGGGTTTTTTCCGCGGCACCGCTCACCCGGGGAAATTA‑‑AAAGTGGGCGGGGAAGCGATTCTGGTTGAATT‑GTTGGATC‑TGCCAAAAGGGCTGGAAGAACGTGCTGATTTCCCCTTGCTTTCACATAATCTGCCCGCCGATTTTGCTGCGTTGCGTAAATTGATGATGAATCATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGGTTGCt      <  2:278361/242‑1 (MQ=255)                                                                                                                                                                                                                                         gTGCGCTGCGTGACCA‑GTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTT‑‑AAAGAGAGCGTGGAAGCGATTCTCGGTGAAAC‑GTTGGATC‑TGCCAAAAGAGCTGGCAGAACGTGCTGATTTACCCTTGCTTTCCCATAATCTGCCCGCCGATTTTGCTGCGTTGCGTCAATTGATGATGAATCCTCATTAAAATATATTCATTATCTCAATCAGGCCGGGTTTTCttttt  >  2:128342/1‑250 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                   TTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGGGCTACACTTCGGAGCCGCACGCTGCCGTAGCTTATCGTGCGCTGCGTGATCA‑GTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTT‑‑AAAGAGAGCGTGGAAGCGATTCTCGGTGAAAC‑GTTGGATC‑TGCCAAAAGAGCTGGCAGAACGTGCTGATTTACCCTTGCTTTCACATAATCTGCCCGCCGATTTTGCTGCGTTGCGTAAATTGATGATGAATCATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTA  >  NC_000913/4577‑5059

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑