Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,618,483	C→T	H85H (CAC→CAT)	deoA →	thymidine phosphorylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,618,483	0	C	T	100.0%	188.6 / NA	63	H85H (CAC→CAT)	deoA	thymidine phosphorylase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (32/31);  total (32/31)

CGCACAAGAAATTATTCGTAAAAAACGTGATGGTCATGCGCTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGTATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACACTCCACCGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTCATACTGGCGGTACGCTCGACAAACTGGAATCCATCCCTGGCTTCGACATTTTCCCGGATGACAACCGTTTCCGCGAAATTATTAAAGACGTCGGCGTGGCGATTATCGGTCAGACCAGTTCACTGGCTC  >  NC_000913/4618237‑4618712                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                      cGCACAAGAAATTATTCGTAAAAAACGTGATGGTCATGCACTGAGCGATGAAGAAAGTCGTTTCTTTACCAACGGCATTCGCGACAACTCTATCTCCGAAGGGCAGATTGCCTCCCTCGCGATGACCATGTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGTAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTccc                                                                                                                                                                                                                                   <  2:204976/251‑2 (MQ=255)       aGAAATTATTCGTAAAAAACGTGATGGTCATGCACTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGCATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTg                                                                                                                                                                                                                             <  1:181985/251‑1 (MQ=255)             tattCGTAAAAAACGTGATGGTCATGCACTGCGCGATGAAGAAATTCGTTTCTTTATCAACGGCATTCGCGACAACAATATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGCCAATGCCAGCGCGTGTCTTGCTGACCATGGCGATGCGCGATTCAGGAACCGTTCTCGACTGGAAACGCCTGCATCTGAATGGCCCGATTGTTGATAAAAATTCCACTGGTGGCgccg                                                                                                                                                                                                                       >  2:362183/1‑248 (MQ=255)             tattCGTAAAAAACGTGATGGTCATGCACTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGCATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTc                                                                                                                                                                                                                        <  1:266717/250‑1 (MQ=255)             tattCGTAAAAAACGTGATGGTCATGCACTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGCATTCACGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCg                                                                                                                                                                                                                       >  1:506489/1‑251 (MQ=255)                        aaCGTGATGGTCATGCCCTGAGCGATGACGAAATTCGTTTCTTTATCAACGGCATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACt                                                                                                                                                                                                            >  1:423830/1‑251 (MQ=255)                             gATGGTCATGCACTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGCATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGCCCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCt                                                                                                                                                                                                       >  1:254832/1‑251 (MQ=255)                                ggTCATGCACTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGCATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGAt                                                                                                                                                                                                    >  1:399190/1‑251 (MQ=255)                                   cATGCACTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGCATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGtt                                                                                                                                                                                                 <  2:178637/251‑1 (MQ=255)                                    aTGCACTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGCATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTg                                                                                                                                                                                                <  1:303401/251‑1 (MQ=255)                                      gcacTGAACGATGAAGAAATTCGTTTCTTTATCAACGGCATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGGTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTACGCTGATGTTgg                                                                                                                                                                                               <  1:383812/247‑1 (MQ=255)                                        acTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGCATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGc                                                                                                                                                                                            >  2:119120/2‑251 (MQ=255)                                         cTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGCATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGTTTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGCGTTCGCTGATGTTGGGGcc                                                                                                                                                                                           >  1:346452/1‑251 (MQ=255)                                           gAGCGATGAAGAAATTCGTTTCTTTATCAACGGCATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGa                                                                                                                                                                                         <  1:371758/251‑1 (MQ=255)                                              cggtGAAGGAATTCGTTTCTTTATCAACGGCATTCGCGACAACACTATCGCCGAAGGGCAGATTGCCGCCCTCGCGATGGCCATTTTCTTCCACCATATGACAATGCCTGAGCGTGTCTCGCTGACCTTGGCTGTGCGAGATTCAGGAACCGTTCTCGTCTGGACACGCCTGCATCTGAATGGCCCGCTTGTTGATAAACATTCCCCAAGTGGCGACGGCGCTGTGACTTCGCTGGTGTTGGGGCCGATgg                                                                                                                                                                                      >  2:144991/4‑251 (MQ=255)                                                   aagaaATTCGTTTCTTTATCAACGGCATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGACCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTGGGGGCCGATGGTctcc                                                                                                                                                                                 >  2:232072/1‑248 (MQ=255)                                                        aTTCGTTTCTTTATCAACGGCATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCATGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGCTAAACATTCCACTGGTGGCGTCGTCGCTGTGACTTCGCTGATGTTGGGGCCGATGGTCGCTGCCTg                                                                                                                                                                            >  1:367932/1‑251 (MQ=255)                                                         ttCGTTTCTTTATCAACGGCATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTgc                                                                                                                                                                           >  1:323926/1‑251 (MQ=255)                                                         ttCGTTTCTTTATCAACGGCATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCATCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCACGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGACGCAGCCTgc                                                                                                                                                                           >  1:146358/1‑251 (MQ=255)                                                           cGTTTCTTTATCAACGGCATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTATCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCcggcgg                                                                                                                                                                         >  2:169944/1‑251 (MQ=255)                                                             tttCTTTATCAACGGCATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGGGGTTCAGGAACCGTTCTCGACTGGAAAAACCTGCATCTGAATGGCCCGATTGTTGATACACATTACACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTgcggcg                                                                                                                                                                       >  1:89387/1‑251 (MQ=255)                                                                   tATCAACGGCATTAGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGCTGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGCTGCGAGATTCAGGTACCGTTCTCGACTGGACAAGCTAGAATATGAAGGGCTCGATTGGTGATAAACATTCCACTGGTGACGTCGGCGTGGTGACTTCGCTGCTGTTGGGGCCGTTGGTCGCAGCGCGCggcggccatc                                                                                                                                                                 >  2:188586/1‑247 (MQ=255)                                                                       aacggctttCGCGAAAACACTATCTCCGATGGGTATATTGCCGCCCTTGTGATGACTATTTTCTTCCAAGAGATGACAATGCCGGTGCGTGGCGCGCTGTCCTGGGCGATGCTAGATTCAGGAATCGTTCTCTACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGGGACTTCGCTGAGGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTcc                                                                                                                                                             <  2:270101/244‑1 (MQ=255)                                                                       aaCGGCATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGGAGGTCGCAGCCTGCGGCGGCTATAtttc                                                                                                                                                             >  2:336836/1‑249 (MQ=255)                                                                            caTTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCgatgt                                                                                                                                                        >  1:70026/2‑250 (MQ=255)                                                                                   gACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTgg                                                                                                                                                 <  1:161995/251‑1 (MQ=255)                                                                                     caacaCTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGTTGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGTTGATCTCTgggc                                                                                                                                               >  2:273074/1‑249 (MQ=255)                                                                                     aaacaCTATCTCCGAAGGGCAGATTGCCGCCCTCGCTATGACCATTTTCTTCCACGATATGACAAGGCCTGTGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTc                                                                                                                                               <  2:274853/250‑1 (MQ=255)                                                                                                ccGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTc                                                                                                                                       <  1:119120/248‑1 (MQ=255)                                                                                                ccGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCg                                                                                                                                      <  1:336836/249‑1 (MQ=255)                                                                                                  gAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGCTGTTCTCTGGTCCCGGCCCCGGTcc                                                                                                                                  >  2:142713/1‑250 (MQ=255)                                                                                                   aaGGGCAGATAGCCGCCCTCGCGATGAACATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTCAt                                                                                                                                 >  1:429651/1‑251 (MQ=255)                                                                                                         agtttggcgctcgcgcgGTGACCAGTTTCTCCCACGATATGACAATGCCAGAGGGTTGCGCGCTGCACATGGCGAGGCGAGAATCAGTAAACGTTCTCGGTTGGAAAAGCCTTCACCTGAAGGGCCCGAGTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTAGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTCAGACTGGc                                                                                                                           <  2:262173/238‑1 (MQ=255)                                                                                                         aGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCCCTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATg                                                                                                                                                                                       >  2:308954/1‑191 (MQ=255)                                                                                                         aGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATg                                                                                                                                                                                       <  1:308954/191‑1 (MQ=255)                                                                                                             tGCCGCCCTAGCGATGACCTTTTTCGTCCCCGATATGACAATGGTTGATCGTGCCTCGCTGACCATGGCGATGCGAGATTCAGGATCCGTTCTCTACTGGAAAAGCCTGCATCTGAATGGCCCGATTGGTGATAAACATTCCGCTGGTGGCGCCGGCGATGTGTCTTCGCTGATGTTGTGGCCGATGGTCTCAGCCTGCGGCGGCGAGATTCCGATGAGCTCTGGTCGCGGCCCCGGTCTTACTGGCGGt                                                                                                                        <  2:59473/250‑1 (MQ=255)                                                                                                                   ccTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGTTCTCTGGTCGCGGCCTCGGTCATACTGGCGGTAccccgg                                                                                                                 >  2:252259/1‑246 (MQ=255)                                                                                                                         gaggcccATTTTTTTCCACTATTTGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCTGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTCATACTGGCGGTACGCTCGACAAAc                                                                                                           <  1:222496/246‑1 (MQ=255)                                                                                                                          aTGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGACGATCTCTGGTCGCGGCCTCGGTCATACTGGCGGGACGCCCGGCAAAcc                                                                                                          >  2:389468/1‑250 (MQ=255)                                                                                                                          aTGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTAATATTGGGGCCGGTGGTCGCAGCCTGCGGCGGCTATATTCCGAAGGTCTCTGGCCGAGGCCTCGGTCATAcatgcggttggctccacacaa                                                                                                           >  1:275358/1‑230 (MQ=255)                                                                                                                           tGACCATTTTCTTCCACGATATGACAATGCCTGTGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGGCGCGGCCTCGGTCATACTTGCgggtcgccccgcaaactc                                                                                                         >  2:78211/1‑235 (MQ=255)                                                                                                                               cATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTCATACTGGCGGTACGCTCGACAAACTGGAat                                                                                                     <  1:248971/251‑1 (MQ=255)                                                                                                                                            gATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTCATACTGGCGGTTCGCTCGACAAACTGGAATCCATCCCTGGCtt                                                                                        >  1:99981/1‑251 (MQ=255)                                                                                                                                                 gACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTCATACTGGCGGGACGCTCGGCAAACTGGAATCCATCCCTGGCTTCGAcc                                                                                   >  1:407109/1‑250 (MQ=255)                                                                                                                                                      tGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTCATACTGGCGGTACGCTCGACCAACTGGAATCCCTCCCTGGCTTCGACAttttt                                                                              >  1:218149/1‑250 (MQ=255)                                                                                                                                                         cagagcgggactcGCAGACCATGTCGAGGCGAATTTAAGGAACCGGTCGCGACTGGAAAAGCCTGCATCTGAATGTCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGAGGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATAGTCCGATGATCTCTGGTCGCGGCATCGGGCATACTGGCGGTACGCTCGCCAAACTGGAATCCATCCCTGGCTTCTACATTTTCCCg                                                                           <  2:146358/241‑1 (MQ=255)                                                                                                                                                               gtgtCTCGCTGCCCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTCATACTGGCGGTACGCTCGACAAACTGGAATCCATCCCTGTCTTCGACATTTTCCCGGATGAc                                                                     <  1:389468/251‑1 (MQ=255)                                                                                                                                                               gtgtCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTgcgg                                                                                                                                                                         <  1:436891/151‑1 (MQ=255)                                                                                                                                                               gtgtCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTgcgg                                                                                                                                                                         >  2:436891/1‑151 (MQ=255)                                                                                                                                                                tgtCTCGCTGACCATTGTGATGCGAGATTCAGGAACCGTTCCCGACTGGAAAAGCCTGAAGCTGAATGGCCCGATTGGTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGTTGTTGGGGCCGATGGTCGCCGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTCATACTGGCGGTACGCTCGACAAACTGGAATCCATCCCTGGCTTCGACATTTTCCCGGATGAc                                                                     <  2:399190/250‑1 (MQ=255)                                                                                                                                                                   ctcGCTGACCATGGCGATGCGGGATTCAGGAACCGTTCTCAACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGAAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTACTACTGGCGGTAATCTCGTCCAACTAGAATTCAACCCCGGCGTCGGAATTTTCCCGGagtcaaacc                                                                 >  1:441375/1‑243 (MQ=255)                                                                                                                                                                   atcgccacccatggcggagccagcgtCAGGATCCGTTCCCGATTGTAAAAGCCTGCATTGGAATGGTCCGATTGTTGATAAACATCCCACTGGTGGCGGCGGGCATGTGACTTCGCTGATGTGGGGTCCGATGGTCGCAGCCTGCGGCGGCGATATGCCGATGATCTCTGGGCGCGTCCGCGGTCATACTGGCGGTACGCTCGACAAACTGGAATCCATCCCTGGCTTCGACATTGTACCGTATGACAAc                                                                  <  2:383391/225‑1 (MQ=255)                                                                                                                                                                         gACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTTATAAACATGCCACTGGTGGCGGCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTCATACTGGCGGTACGCTCGACAAACTGGAATCCATCCCTGGCTGCGGCATTTTCCCGGATGACAACCGTTTcc                                                           <  2:64222/251‑1 (MQ=255)                                                                                                                                                                              tGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTCATACTGGCGGTACGCCCGACACACTGGAATCCCTCCCTGGGTTCGGCATTTTCCCGGGTGACAACCGGTTCcgcgg                                                       >  1:185852/1‑249 (MQ=255)                                                                                                                                                                               ggcagagCGAGATTCAGGAACCCTTTTCGACTGGAAAATCCTTCATCGGACTGGCCCGATTGTTGATAAACATGCCACTGGTGGCGTCGGCGATGTTACTCCGCTTATGTTGGGGCCGATGGTCGCTGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTCATACTGGCGGTACGCGCGACAAACTTGAAGCCAACACTGGCTTCGTCATTTTCCCGGATGATAACCGTTGCCGCGaaa                                                     <  2:55006/245‑1 (MQ=255)                                                                                                                                                                                        agaTTCAGGAACCGTTTCCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCTCTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTCATACTGGCGGTACGCTCGACAAACTGGAATCCATCCCTGGCTTCGACATTTTCCCGGATGACAACCGTTTCCGCGAAATTATTAAAg                                            <  2:423830/251‑1 (MQ=255)                                                                                                                                                                                         agatccggAACCCTTCTCCACTGGAAAAGCCTGCCTGTGAATGGCCCGGTTTTTTAATAACCTTCCCCTGGTGGGGTCCGGGGTTTGAATTCCCTGATTGTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTCATACTGGCGGTACGCTCGACAAACTGGAATCCATCCCTGGCTTCGACATTTTCCCGGATGACAACCGTTTCCGCGAAATTATTAAAGa                                           <  1:324351/245‑1 (MQ=255)                                                                                                                                                                                                 aaCCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTCATACTGGCGGTACGCTCGACAAAATGGAATCCATCCCTGGCTTCGACATTTTCCCGGATGACAACCGTTTCCGCGAAATTATTAAAGACGTCGGCg                                   >  1:216438/1‑251 (MQ=255)                                                                                                                                                                                                                gAAAAGCCTGCATTGGAATGGCCCGCTTGTTGATAAACATTCCTCGGGGGGCGTCGGCGATGGGACTGCGCTGATGTTGGTGCAGATGGGTGCAGCCTGCGGCGGCTATTTTCCGAGGATCTCTGGCCGCGGCCTCGGGCATACTGGCGGTACGCTCGACAAACTGGAATCCATCCCTGGCTTCGACATTTTCCCGGATGACAACCGTTGCCGCGAAATTATTAAAGTCGTCGGCGTGGCGATTATCGGTc                    <  2:275358/251‑1 (MQ=255)                                                                                                                                                                                                                    aGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTCATACTGGCGGTACGCTCGACAAACTGGAATCCATCCCTGGCTTCGACATTTTCCCAGATGACAACCGTTTCCGCGAAATTATTAAAGACGTCGGCGTGGCGATTATCGGTCAGAc                <  1:269478/251‑1 (MQ=255)                                                                                                                                                                                                                               gAATGGCCCGATTGTTTATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTCATACTGGCGGTACGCTCGACAAACTGGAATCCATCCCTGGCTTCGACATTTTCCCGGATGACAACCGTTTCCGCGAAATTATTAAAGACGTCGGCGTGGCGATTATCGGTCAGACCAGCTCACt       <  2:323926/249‑1 (MQ=255)                                                                                                                                                                                                                               gAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTCATACTGGCGGTACGCTCGACAAACTGGAATCCATCCCTGGCTTCGACATTTTCCCGGATGACAACCGTTTCCGCGAAATTATTAAAGACGTCGGCGTGGCGATTATCGGTCAGACCAGCTCACTgg     <  1:169944/251‑1 (MQ=255)                                                                                                                                                                                                                                   ggCCCGATTGTCGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTCATACTGGCGGTACGCTCGACAAACTGGAATCCATCCCTGGCTTCGACATTTTCCCGGATGACATCCGTTTCCGCGAAATTATTAAAGACGTCGGCGTGGCGATTATCGGTCAGACCAGCTCACTGGCTc  <  1:397288/250‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                      CGCACAAGAAATTATTCGTAAAAAACGTGATGGTCATGCGCTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGTATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACACTCCACCGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTCATACTGGCGGTACGCTCGACAAACTGGAATCCATCCCTGGCTTCGACATTTTCCCGGATGACAACCGTTTCCGCGAAATTATTAAAGACGTCGGCGTGGCGATTATCGGTCAGACCAGTTCACTGGCTC  >  NC_000913/4618237‑4618712

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑