Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,618,075	C→T	G251G (GGC→GGT)	deoC →	deoxyribose‑phosphate aldolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,618,075	0	C	T	98.4%	190.7 / NA	63	G251G (GGC→GGT)	deoC	deoxyribose‑phosphate aldolase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (26/36);  minor base G (0/1);  total (26/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAGTGGCTGTGAACGCGACGCCGGAAAGCGCGCGCATCATGATGGAAGTGATCCGTGATATGGGCGTAGAAAAAACCGTTGGTTTCAAACCGGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATTGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGCGCTTCCAGCCTGCTGGCAAGCCTGCTGAAAGCGCTGGGTCACGGCGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTCCTCGGGAGGTTACCTTGTTTCTCGCACAAGAAATTATTCGTAAAAAACGTGATGGTCATGCGCTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGTATTCGCG  >  NC_000913/4617837‑4618319                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                     aaGTGGCTGTGAACGCGACGCCGGAAAGCGCGCGCAGCATGATGGAAGTGATCCGTGATATGGGCGTAGAAAAAACCGTTGGTTTCAAACCAGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATCGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGACGGTAAGAgc                                                                                                                                                                                                                                          <  1:8151/251‑1 (MQ=255)     ggCTGTGAACGCGACGCCGGAAAGCGCGCGCATCATGATGGAAGTGATCCGTGATATGGGCGTAGAAAAAACCGTTGGTTTCAAACCAGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATCGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGACGGTAAGAGCGCca                                                                                                                                                                                                                                      <  2:248188/251‑1 (MQ=255)        ggtgAACGCGACGACGGAAAGCGCGCGCATCATGATGGAAGTGATCCGTGATATGGGCGTAGAAAAAACCGTTGGTTTCAAACCAGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATCGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACCACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGACGGTAAGAGCGCcagca                                                                                                                                                                                                                                   <  1:275757/250‑1 (MQ=255)            aaCGCGACGCCGGAAAGCGCGCGCATCATGATGGAAGTGATCCGTGATATGGGCGTAGAAAAAACCGTTGGTTTCAAACCAGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATCGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGcta                                                                                                                                                                                                                               <  2:175420/251‑1 (MQ=255)                 gACGCCGGAAAGCGCGCGCATCATGATGGAAGTGATCCGTGATATGGGCGTAGAAAAAACCGTTGGTTTCAAACCAGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATCGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCCGctacta                                                                                                                                                                                                                            >  2:303401/1‑249 (MQ=255)                    gCCGGAAAGCGCGCGCATCATGATGGAAGTGATCCGTGATATGGGCGTAGAAAAAACCGTTGGTTTCAAACCAGCGGGCGGCGTGCGTACGGCGGAAGATGCGCAGAAATATCTCGCCATCGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACtaagtaa                                                                                                                                                                                                                       <  2:265940/251‑1 (MQ=255)                       ggAAAGCGCGCGCATCATGATGGAAGTGATCCGTGATATGGGCGTAGAAAAAACCGTTGGTTTCAAACCAGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATCGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGGTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGAt                                                                                                                                                                                                                    <  1:132117/251‑1 (MQ=255)                          aaGCGCGCGCATCATGATGGAAGTGATCCGTGATATGGGCGTAGAAAAAACCGTTGGTTTCAAACCAGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATCGCAGATGAACTGTGCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCt                                                                                                                                                                                                                 <  2:169116/251‑1 (MQ=255)                           aGCGCGCGCATCATGATGGAAGTGATCCGTGATATGGGCGTAGAAAAAACCGTTGGTTTCAAACCAGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATCGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCtt                                                                                                                                                                                                                >  1:269110/1‑251 (MQ=255)                                 cgcATCATGATGGAAGTGATCCGTGATATGGGCGTAGAAAAAACCGTTGGTTTCAAACCAGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATCGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGcc                                                                                                                                                                                                          <  1:171057/251‑1 (MQ=255)                                 cgcATCATGATGGAAGGGATCCGTGATATGGGCGTAGAAAAAACCGTTGGTTTCAAACCAGCGGGCGGCGGGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATCGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGcc                                                                                                                                                                                                          <  2:58728/251‑1 (MQ=255)                                      catGATGGAAGTGATCCGTGATATGGGCGTAGAAAAAACCGTTGGTTGCAAACCAGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATCGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGACGGTAAGAGCTCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGAt                                                                                                                                                                                                      <  1:384185/250‑1 (MQ=255)                                       atgatgGAAGTGATCCGTGATATGGGCGTAGAAAAAACCGTTGGTTTCAAACCAGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATCGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGAtgc                                                                                                                                                                                                    >  1:248399/1‑251 (MQ=255)                                          attgAAGTGATCCGTGATATGGGCGTAGAAAAAACCGATGGTTTCAAACCAGCGGGCGGCGGGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATCGCGGATGAGCTGTGCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTGAAAGCGCTTGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGAtgcgct                                                                                                                                                                                                 <  2:12560/248‑1 (MQ=255)                                                   aTCCGTGATATGGGCGTAGAAAAAACCGTTGGTTTCAAACCCGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATCGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATc                                                                                                                                                                                        >  2:327037/1‑251 (MQ=255)                                                      cGTGATATGGGCGTAGAAAAAACCGTTGGTTTCAAACCAGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATCGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAgg                                                                                                                                                                                     >  1:323521/1‑251 (MQ=255)                                                              gggCGTAGAAAAAACCGTTGGGTTCAAACCAGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATCGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACgag                                                                                                                                                                             <  2:229104/251‑1 (MQ=255)                                                                  gTAGAAAAAACCGTTGGGTTCAAACCAGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATCGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGACGTTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGt                                                                                                                                                                         <  1:233540/251‑1 (MQ=255)                                                                        aaaaCCGTTGGTTTCAAACCAGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATCGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGGAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTAc                                                                                                                                                                   >  2:266717/1‑251 (MQ=255)                                                                                 ggTTTCAAACCAGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATCGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGcc                                                                                                                                                          >  1:144794/1‑251 (MQ=255)                                                                                              cgggcggCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATCGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGt                                                                                                                                               >  1:484448/1‑249 (MQ=255)                                                                                              cgggcggCGTGCGTACTGCGGAAGATGCGCAGAAATATCGCGCCATCGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAg                                                                                                                                             <  1:416567/251‑1 (MQ=255)                                                                                                ggcggcGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATCGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGAc                                                                                                                                           >  1:285330/1‑251 (MQ=255)                                                                                                      gtgcgtACTGCGGAAGATGCGCAGAAATATCTCGCCATCGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATcc                                                                                                                                     <  1:327037/251‑1 (MQ=255)                                                                                                       tgcgtACTGCGGAAGATGCGCAGAAATATCTCGCCATCGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCTGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCg                                                                                                                                    >  1:59473/1‑251 (MQ=255)                                                                                                           tACTGCGGAAGATGCGCAGAAATATCTCGCCATCGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGGCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCACGCCTGTTGAAGGCGCGGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGCTGCGCTGCGATTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCa                                                                                                                                 >  1:400856/1‑250 (MQ=255)                                                                                                                   aagaagCGCAGAAATATCCCGCCATAGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAgccgcc                                                                                                                        <  2:185888/251‑1 (MQ=255)                                                                                                                         cgcAGAAATATCTCGCCACCGCAGATTAACTGTTCGTGGCTTACTGGGCAGATGCGCGTCACTACCGTTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTGAAAGCGCTGTGCCTCGGTGACTTTAGGAGCGCCAGCAGCTACTAAGTAATATGCTTTACGCCTGAGGCGCTGCTCTTATCAGTCCTACGAGACGTATCTACACGTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAGCCGCCTCAttc                                                                                                                  <  1:311866/251‑3 (MQ=255)                                                                                                                           cAGAAATATCTCGCCATCGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAGCCGCCTCATActc                                                                                                                <  1:88785/251‑1 (MQ=255)                                                                                                                             gAAATCTCTCGCCATCGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTGAACGCGCTGGGACACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCACAAGCCGCCTAATACTCtt                                                                                                              >  2:324351/1‑251 (MQ=255)                                                                                                                               aaTATCTCGCCATCGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAGCCGCCTCATACTCtttt                                                                                                            <  1:43075/251‑1 (MQ=255)                                                                                                                                     tcGCCATCGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTCCCCGTAGCCCGGATAAGGCGTAGTCGCATCCGGCAACAGCCGCCTCATACTCTTTTCCTCgg                                                                                                      >  1:167582/1‑251 (MQ=255)                                                                                                                                           ccgCAGATGAACTGTTCGGTGCTGACTGGGCATATGCGCGTCACTACCGCTTTGGTGCGTCCAGCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACTCCTGATGCGCTTCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGAGAAGGCGTAGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTCCTCGGGAGGtt                                                                                                <  1:450691/249‑1 (MQ=255)                                                                                                                                             gCAGATGAACGGTGCGGTGTTGACTGGGCAGATGCGCGTCATTACCGCTTTGGTGCTTCGAGCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTAGCAGGCCTACGAGACGTATCGACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTCCTCGGGAGGTTAc                                                                                              <  2:44433/251‑1 (MQ=255)                                                                                                                                               aGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTCCTCGGGAGGTTACCt                                                                                            <  1:397011/251‑1 (MQ=255)                                                                                                                                                    aaCTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTCCTCGGGAGGTTACCTTGttt                                                                                       >  1:122879/1‑251 (MQ=255)                                                                                                                                                     cctgttcgtgcatggcTGGGCAGATGCTCGTCTCTTCCGCTTTGGGTCTTCCAGCCGGCTGGTAAGCCTGTTGAAAGCGCTGGGCCACGGTGACGGTAAGAGGCCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCGGCGCTTATCAGGCCTACGAGACGTATCTTCCCGGAGGCCGGATAAGGCGTAGACGCATCTGGCAAAAGCCGCCTCATACTCTTTTCCTCGGGAGGTTACCTTGTTtc                                                                                      <  2:456359/236‑1 (MQ=255)                                                                                                                                                          tCGGTGCTGACTGGGCAGATGCGCGTCACTACCGATTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGCCGCATCCGGCAACAGCCGCCTCATACTCTTTTCCTCGGGAGGTTACCTTGTTTCTCGcc                                                                                 >  2:371758/1‑250 (MQ=255)                                                                                                                                                           cGGTGCTGACTGGGCAGATGCGCGTCACTGCCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTGAAAGCGCGGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTAGCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTCCTCGGGAGGTTACCTTGTTTCTCGca                                                                                 <  2:138646/250‑1 (MQ=255)                                                                                                                                                               gCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTCCTCGGGAGGTTACCTTGTTTCTCGcaca                                                                               >  1:274853/1‑248 (MQ=255)                                                                                                                                                                 tGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTCCTCGGGAGGTTACCTTGTTTCTCGCACAAGaa                                                                           >  2:147102/1‑250 (MQ=255)                                                                                                                                                                   aCTGGGCAGATGCGCGTCACTAACGCTTTAATGATTCCAGCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCCGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTCCCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTCCTCGAGAGGTTACCTTGTTTCTCGCACACGAAatt                                                                        >  2:486761/1‑251 (MQ=255)                                                                                                                                                                     ggggaagatgcgctgaccTACCGCGTTAGTGCTTCCAGCCTGCTGGCAAGCCTGTTGAAATCGCTGGGCTTCGGTGACGGGAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTCCTCGGGAGGTTACCTTGTTTCTCGCACAAGAAattat                                                                      <  2:340986/234‑1 (MQ=255)                                                                                                                                                                      gggCAGATGCGCGTCACTTCCGCTTTTGTGCTTCCAGCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTCCTCGGGAGGTTTCCTTGTTTCTCGCACAAGAAattatt                                                                     <  1:218496/251‑1 (MQ=255)                                                                                                                                                                       ggCAGAGGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTCCTCGGGAGGTTACCTTGTTTCTCGCACAAGAAATTATTc                                                                    <  2:248399/251‑1 (MQ=255)                                                                                                                                                                        gCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTCCTCGGGAGGTTACCTTGTTTCTCGCACAAGAAATTATTCg                                                                   <  2:479716/251‑1 (MQ=255)                                                                                                                                                                            aTGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTCCTCGGGAGGTTACCTTGTTTCTCGCACAAGAAATTATTCGTaa                                                                >  1:45908/1‑250 (MQ=255)                                                                                                                                                                                 cgTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTCCTCGGGAGGTTACCTTGTTTCTCGCAACAGAAATTATTCGTaaaaaa                                                            >  2:8671/1‑249 (MQ=255)                                                                                                                                                                                      cTACCGCATTGGCGACTCCAGCCTGCTGGCAATCCTCTTGAACGAGCTGGACCACGGTGACGGTAAGCGCGCCAGCAGCTACTCAGAAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGCAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAGCCGCCTCATACTATTTTCATCGGGAGGTTACCTTGATTCTCGCACAAGAAATTACTCGTAAAAAACgggctt                                                     >  2:189000/1‑246 (MQ=255)                                                                                                                                                                                           gCTTTGGTGCTTCCATCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTCCTCGGGAGGTTACCTTGTTTCTCGCACAAGAAATTATTCGTAAAAAACGTGATGGTCAt                                                <  1:196459/251‑1 (MQ=255)                                                                                                                                                                                                ggtgtttCCAGCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTCCTCGGGAGGTTACCTTGTTTCTCGCACAAGAAATTATTCGTAAAAAACGTGATGGTCATgcact                                           <  1:453024/246‑4 (MQ=255)                                                                                                                                                                                                 gtgcTTCCAGCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTCCTCGGGAGGTTACCTTGTTTCTCGCACAAGAAATTATTCGTAAAAAACGTGATGGTCATGCACTg                                          <  1:340671/249‑1 (MQ=255)                                                                                                                                                                                                       ccAGCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCCGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCAACGAGCCGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCTGCAACAGCCGCCTCATACTCTTTTCCTCGGGAGGTTACATTGTTTCTCGCACAAGAAATTATTCGTAAGAAACGTGATGGTCATGAACTGAGCGAt                                    >  2:349293/1‑251 (MQ=255)                                                                                                                                                                                                        cAGCATGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGCGACGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAACAGCCTCATACTATTTTCCTCGGGAGGTTACCTTGTTTCTCGCACCAGAAATTATTCGTAAACAACGTGATGGTCATGCACTGCGCGATg                                   >  2:383812/1‑251 (MQ=255)                                                                                                                                                                                                               ctgGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAgcc                                                                                                                           <  1:483066/156‑1 (MQ=255)                                                                                                                                                                                                               ctgGCAAGACTGTTGAAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGACTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAAACgcc                                                                                                                           >  2:483066/1‑156 (MQ=255)                                                                                                                                                                                                                 ggCAAGCCTGTTGAAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTCCTCGGGAGGTTACCTTGTTTCTCGCACAAGAAATTATTCGTAAAAAACGTGATGGTCATGCACTGAGCGATGAAGAAAtt                           <  1:319775/250‑1 (MQ=255)                                                                                                                                                                                                                    aaGCCTGTTGAAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTCCTCGGGAGGTTACCTTGTTTCTCGCACAAGAAATTATTCGTAAAAAACGTGATGGTCATGCACTGAGCGATGAAGAAATTCGtt                       <  2:45908/251‑1 (MQ=255)                                                                                                                                                                                                                     aGCCTGTTGAAAGCGCTGGGCCACGGGGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTCCTCGGGAGGTTACCTTGTTTCTCGCACAAGAAATTATTCGTAAAAAACGTGATGGTCATGCACTGAGCGATGAAGAAAGTCGttt                      <  2:388752/251‑1 (MQ=255)                                                                                                                                                                                                                       ccTGTTGAAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTCCTCGGGAGGTTACCTTGTTTCTCGCACAAGAAATTATTCGTAAAAAACGTGATGGTCATGCACTGAGCGATGAAGAAATTCGTTTCt                    >  1:385237/1‑251 (MQ=255)                                                                                                                                                                                                                         tgttgAAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTCCTCGGGAGGTTACATTGTTTCTCGCACAAGAAATTATTCGTAATAAACGTGATGGTCATGCACTGCGCGAAGAAGAAATTCGTTTCttt                  >  2:85794/4‑251 (MQ=255)                                                                                                                                                                                                                            ggAAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTCCTCGGGAGGTTACCTTGTTTCTCGCACAAGAAATTATTCGTAAAAAACGTGATGGTCATGCACTGAGCGATGAAGAAATTCGTTTCTTTAt                <  1:17086/249‑1 (MQ=255)                                                                                                                                                                                                                                         ccACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTCCTCTGGAGGTTACCTTGTTTCTCGCACAAGAAATTATTCGTAAAAAACGTGATGGTCATGCACTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGCATTcgcg  <  1:284685/250‑1 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                     AAGTGGCTGTGAACGCGACGCCGGAAAGCGCGCGCATCATGATGGAAGTGATCCGTGATATGGGCGTAGAAAAAACCGTTGGTTTCAAACCGGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATTGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGCGCTTCCAGCCTGCTGGCAAGCCTGCTGAAAGCGCTGGGTCACGGCGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTCCTCGGGAGGTTACCTTGTTTCTCGCACAAGAAATTATTCGTAAAAAACGTGATGGTCATGCGCTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGTATTCGCG  >  NC_000913/4617837‑4618319

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑