Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,804	T→G	A357A (GCT→GCG)	thrC →	threonine synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,804	0	T	G	100.0%	98.9 / NA	37	A357A (GCT→GCG)	thrC	threonine synthase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (16/21);  total (16/21)

AACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGGGCTACACTTCGGAGCCGCACGCTGCCGTAGCTTATCGTGCGCTGCGTGATCA‑GTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTT‑‑AAAGAGAGCGTGGAAGCGATTCTCGGTGAAAC‑GTTGGATC‑TGCCAAAAGAGCTGGCAGAACGTGCTGATTTACCCTTGCTTTCACATAATCTGCCCGCCGATTTTGCTGCGTTGCGTAAATTGATGATGAATCATCAGTAAAATCTATTCATTATCTCAATCAGG  >  NC_000913/4559‑5044                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                      ttccgagcggtgcccctttcccagCACGACGGGCAGTGTTCACCCAAAGCGCCTCAGGCGACGTTACCCAACGCGATGGACGGGAGTCAGCCAAACAACTGGCGGCGTGTGGAAGATTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGGGCTACACCTCGGAGCCGCACGCTGCCGTAGCGTATc                                                                                                                                                                                                                                                   <  2:394919/227‑1 (MQ=255)          gTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCACAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGGGCTACACCTCGGAGCCGCACGCTGCCGTAGCGTATc                                                                                                                                                                                                                                                   >  1:394919/1‑241 (MQ=255)                   ttCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGGGCTACACCTCGGAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCA‑Gtt                                                                                                                                                                                                                               >  1:350103/1‑251 (MQ=255)                       tGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGGGCTACACCTCGGAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCA‑GTTGAAt                                                                                                                                                                                                                           >  1:437112/1‑251 (MQ=255)                                          cccaaaaGCGACTCAGGGAACGTTACCCAAGGGGTGGGACGTGAGTCAGCCGAACAACTGGCCGGGTGGGAAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGGGCTACACCTCGGAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCA‑GTTGAATCCAGGCGAATATGGCTTGt                                                                                                                                                                                                        <  2:484471/247‑1 (MQ=255)                                            ccAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGCACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGGGCTACAACTCGGAGCAGCACGCTGCCGTAGCGTATCGTGCGCTGCG‑GAACAAGTTGAATCCAGGCGAATATGGCTTGTTc                                                                                                                                                                                                      >  1:381167/1‑251 (MQ=255)                                                                      aaCGCGATGGACGTGATTCAGCCGCACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGCCAATGCGTGAGTTAAAAGAACTGGGCTACACCTCGGAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCA‑GTTGAATCCCGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGGAAtt                                                                                                                                                                            >  1:450085/1‑251 (MQ=255)                                                                                     aGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGGGCTACACCTCGGAGCCGCACGCTGCCGTAGCGAATCGTGCGCTGCGTGACCA‑GTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCCCGCCTCCGGCGAAATTT‑‑AAAGAGAGCggggg                                                                                                                                                           >  2:360378/1‑247 (MQ=255)                                                                                      gTCAGCCGAACAACGTGCCGGGGGTGGAAGGGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGGGCTACACCTCGGAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCA‑GTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTT‑‑AAAGAGAGCGTGGaa                                                                                                                                                          <  2:250062/251‑1 (MQ=255)                                                                                       tCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAACTCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAAACAAGCAACAGACAATGAGGGAGTTAAAAGAACTGGGCTACACCTCGGAGCCGCACGCAGCCGTAGCGAATCGGGCGCTGCGTGACCA‑GTTGAATCCAGGCGAATGTGGATTGTTCCTCAGCACCTCACATCCGGCGACATTTCAAAagagagagaa                                                                                                                                                             >  2:78303/1‑245 (MQ=255)                                                                                         cgccgaccactgtgCCGCGTGTGTAAGAGTGGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGGGCTACACCTCGGAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCA‑GTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATGT‑‑AAAGAGAGCGTGGAAGCg                                                                                                                                                       <  2:441408/238‑1 (MQ=255)                                                                                               acaccTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGGGCTACACCTCGGAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCA‑GTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTT‑‑AAAGAGAGCGTGGAAGCGATtctc                                                                                                                                                 <  1:469407/247‑1 (MQ=255)                                                                                               acaatgggccgggggtgGAAGTGTGGTTCCGCCGCAAAAACTGTCTACTGAAAGAGCTGGGTTATGCAGCCGTTGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGGGCTATACCTCGGAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCA‑GTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTT‑‑AAAGAGAGCGTGGAAGCGATtctc                                                                                                                                                 <  2:484006/237‑1 (MQ=255)                                                                                                   cTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGGGCTACACCTCGGAGCCGCACGCTGCCGTAGCGTATCGt                                                                                                                                                                                                                                                 <  1:200152/154‑1 (MQ=255)                                                                                                   cTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGGGCTACACCTCGGAGCCGCACGCTGCCGTAGCGTATCGt                                                                                                                                                                                                                                                 >  2:200152/1‑154 (MQ=255)                                                                                                      gCCGCGTGGGAAAGGGTGGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCGGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGGGCTACACCTCGGAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCA‑GTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTT‑‑AAAGAGAGCGTGGAAGCGATTCTCGGTGaaa                                                                                                                                          <  2:6450/251‑1 (MQ=255)                                                                                                       ccGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGGGCTACACCTCGGAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCA‑GTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTT‑‑AAAGAGAGCGTGGAAGCGATTCTCGGTGAAAc                                                                                                                                         <  1:182889/251‑1 (MQ=255)                                                                                                                          tCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGGGCTACACCTCGGAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCA‑GTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTT‑‑AAAGAGAGCGTGGAAGCGATTCTCGGTGAAAC‑GTTGGATC‑TGCCAAAag                                                                                                                      >  1:83681/1‑249 (MQ=255)                                                                                                                                         ggaaaCTGAAAGAGCGTGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGGTAAAAGAACTGGGCGACACCTCGGGGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCA‑GTTGAATCCAGGCGAATATGGCGTGTTCCTCGGCACCGCGCATCCGGCGAAATTG‑‑AAAGAGAGCGTGGAAGCGATTCTCGGTGAAAC‑GTTGGATC‑TGCCAAAAGAGCTGGCAGAACGTGc                                                                                                      <  2:255114/247‑1 (MQ=255)                                                                                                                                                   agagCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGGGCTACACCTCGGAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCA‑GTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTT‑‑AAAGAGAGCGTGGAAGCGATTCTCGGTGAAAC‑GTTGGATC‑TGCCAAAAGAGCTGGCAGAACGGGCTGATTTACCCt                                                                                           >  1:267811/1‑251 (MQ=255)                                                                                                                                                                        ggATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGGGCTACACCTCGGAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCA‑GTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTT‑‑AAAGAGAGCGTGGAAGCGATTCTCGGTGAAAC‑GTTGGATC‑TGCCAAAAGAGCTGGCAGAACGTGCTGATTTACCCTTGCTTTCACATAATCTGccc                                                                       <  1:75739/250‑1 (MQ=255)                                                                                                                                                                         gatgatGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGGGCTACCCCTCGGAGCCGCACGCTGCCGTAGCGCATAGTGCGCTGCGTGCCCA‑GTTGAATCCAGCCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGACGAAATTT‑‑AAAGAAAGCGTGGAAGCGATTCTCGGTGAAAC‑GTTGGATC‑TGCCAAAAGAGCTGGCAGACCGTGCTGCTTTAACCCTGCTTTCACATAATCTGCccgc                                                                     >  1:484427/1‑251 (MQ=255)                                                                                                                                                                                                   gCGTGAGTTAAAAGAACTGGGCTACACCTCGGAGCCGCCCGCTGCCGTAGCGTATCGTGCGCTGCGTGACCA‑GTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTT‑‑AAAGAGAGCGTGGAAGCGATTCTCGGTGAAAC‑GTTGGATC‑TGCCAAAAGAGCTGGCAGAACGTGCTGATTTACCCTTGCTTTCACATAATCTGCCCGCCGATTTTGCTGCGTTGCGTAAATtg                                            <  1:344557/250‑1 (MQ=255)                                                                                                                                                                                                      ggattaaaaaaaACTGGCCTACTCCTCGGGGCCGCACGCGGCCGTAGCGTTTCGTGCGCTGCGTGACCA‑GTTGATTCCAGGCGAATATGGCTTGTTCCTCGGCCCCGCGCATCCGGCGAAATTT‑‑AAAGAGAGCGTGGAAGCGTTTCTCGGTGAACC‑GTTGGATC‑TGCCAAAAGAGCTGGCAGAACGTGCTGATTTACCCTTGCTTTCACATAATCTGCCCGCCGATTTTGCTGCGTTGCGTAAATtgatga                                        <  1:367367/245‑1 (MQ=255)                                                                                                                                                                                                           aaaaagacgtgggctccccccgggAGCCGCCCGCCGCCTTAGCGTATCGTGCGCTGCGGGACCA‑GTTAAATCCAGGAAAATAGGGCTTTTTCCCCGGCACCGCGCACCCGGCGAAATTA‑‑AAAGAGAGGGTGGAAGCGATTCTCGG‑GAAACCGTTGGATC‑TGCCAAAAGGGCTGGCAGAACGTGCTGATTTACCCTTGTTTTAACAGAATCTGCCCGCCGATTTTGCTGCGTTGCGTAAATTGATGATGaa                                    <  2:437112/228‑1 (MQ=255)                                                                                                                                                                                                               aGAACTGGGCTACACCTCGGAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCA‑GTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTT‑‑AAAGAGAGCGTGGAAGCGATTCTCGGTGAAAC‑GTTGGATC‑TGCCAAAAGAGCTGGCAGAACGTGCTGATTTACCCTTGCTTTCACATAATCTGCCCGCCGATTTTGCTGCGTTGCGTAAATTGATGATGAatcatc                               <  1:150570/251‑1 (MQ=255)                                                                                                                                                                                                                    ggggCTACCCCTCGGAGCCGCACGCTGCGGTAGCGTATCGTGCGCTGCGTGACCA‑GTTGAATCCAGGCGAATATGGCTTGTTCCCCGGCACCGCGCATCCGGCGAAATTT‑‑AAAGAGAGCGTGGAAGCGATTCTCGGTGAAAC‑GTTGGATC‑TGCCAAAAGAGCTGGCAGAACGTGCTGATTTCCCCTTGCTTTCACATAATCTGCCCGCCGATTTTGCTGCGTTGCGTAAATTGATGATGAATCATCAGTaa                          <  1:492819/250‑1 (MQ=255)                                                                                                                                                                                                                     gggctccccctCGGAGCCGCCCGCTGCCGTAGCGTATCGTGCGCTGCGTGACCA‑GTTGAATCCAGGCGAATATGTCTTGTTCCTCGGCACCGCCCATCCGGCGAAATTT‑‑AAAGAGAGCGTGGAAGCGATTCTCGGTGAAAC‑GTTGGATC‑TGCCAAAAGAGCTGGCAGAACGTGCTGATTTCCCCTTGCTTTCACATAATCTGCCCGCCGATTTTGCTGCGTTGCGTGAATTGATGATGAATCATCAGTaaa                         <  1:63385/241‑1 (MQ=255)                                                                                                                                                                                                                        cTACACCTCGGAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCA‑GTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTT‑‑AAAGAGAGCGTGGAAGCGATTCTCGGTGAAAC‑GTTGGATC‑TGCCAAAAGAGCTGGCAGAACGTGCTGATTTACCCTTGCTTTCACATAATCTGCCCGCCGATTTTGCTGCGTTGCGTAAATTGATGATGAATCATCAGTAAAATc                      >  1:79790/1‑251 (MQ=255)                                                                                                                                                                                                                          acacCTCGGGGCCGGACGATGCAGTAGCGGATCGTGCGCTGAGTGACCC‑GTTGAATCCAGGCGAATATGGCTTGGTCCTCGGCACCGCGCATCCGGCGAAATTT‑‑AAAGAGAGCGTGGAAGCGAGTCTCGGTGAAAC‑ATTGGATC‑TGCCAAAAGAGCTGGCAGAACGTGCTCATTTACCCTTGCTTTCGCATAGTCTGCCCGCCGGTTTTGCTGCGTTGTGGAAATTGCTTAAGAATCATCAGTAAActct                     >  2:90595/1‑250 (MQ=255)                                                                                                                                                                                                                           cacctcggaaacGCACGCTGCCGAAGCGTATCGTGCGCTGCATCGCCA‑GTTGAATCCAGGCGAATATGGATTGTTCCTCGGCACCGCGCATCCGGCGAAATTT‑‑CAAGCGAGAGTGAAAGCGCTTCTCGGTGAAAC‑GGTGGATCATACCTAA‑GAACCTGCAGAACGTGCTGATTTACCCTTGCTTTCACATCATCTGCCCGCCGCTTTTTCTGCGTTTAGAAACTTGATGGTGAATCATCAGTaaacactat                   >  2:459910/12‑245 (MQ=255)                                                                                                                                                                                                                                  gAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCA‑GTTGAATACAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTT‑‑AAAGAGAGCGTGGAAGCGATTCTCGGTGCAAC‑GTTGGATC‑TGCCAAAAGAGCTGGCAGAACGTGCTGATTTACCCTTGCTTTCACATAATCTGCCCGCCGATTTTGCTGCGTTGCGTAACTTGATGGTGAATCATCAGTAAAATCTATTCATTAt            >  1:447430/1‑251 (MQ=255)                                                                                                                                                                                                                                    gCCGCACGCTGCTGTAGCGTATCGTGTGCTGCGTGACCA‑GTTGAATCCAGGAGAATAGGGCTTGTACCTCGGCACCGCGCATCCGGCGAAATTT‑‑AAAGAGAGCGTGGAAGCGATTCTCGGTGAATC‑GTTGGATC‑TGCCAAAAGAGCTGGCAGACCGTGCTGATTTCCCCTTGCTTTCACATAATCTGCCCGCCGATTTTGCTGCGTTGCGTAAATTGATGATGAATCATCAGTAAAATCTATTCATTAtct          <  1:7692/251‑1 (MQ=255)                                                                                                                                                                                                                                       ccgctcTGCCGGAGCGTATCGTGCGCTGCGTAACCT‑GTTGAATCAAGGCGAATATGGCTTGTTCCTCGGCCCCGCGCATCGGGCGAAATTT‑‑AAAGAGGGCTTGGAAGCGATTCTCGGTGAAAC‑TTTGGATC‑TGCCAAAAGAGCTGGCAGAACGTGCTGTTTTACCCTTGCTTTCACATAATCTGCCCGCCGATTTTGCTGCGTTGCGTAAATTGATGATGAATCATCAGTAAAATCTATTCATTATCTCaa       <  1:138244/246‑1 (MQ=255)                                                                                                                                                                                                                                        caggCTGCCGTAGCGTATCGTGCGCTGCGTGACCA‑GTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTT‑‑AAAGAGAGCGTGGAATCGATTCTCGGTGAAAC‑GTTGGATC‑TGCCAAAAGAGCGGGCAGAACGTGCTGATTTCCCCTTGCTTTCACATAATCTGCCCGCCGATTTTGCTGCGTTGCGTAAATTGATGATGAATCATCAGTAAAATCTATGCATTATCTCAAt      <  1:114087/248‑1 (MQ=255)                                                                                                                                                                                                                                           gCTGCCGTAGCGTATCGTGCGCTGCGTGACCA‑GTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTT‑‑AAAGAGAGCGTGGAAGCGATTCTCGGTGAAAC‑GTTGGATC‑TGCCAAAAGAGCTGGCAGAACGTGCTGATTTACCCTTGCTTTCACATAATCTGCCCGCCGATTTTGCTGCGTTGCGTAAATTGATGATGAATCATCAGTAAAATCTATTCATTATCTCAATc     >  1:389692/1‑249 (MQ=255)                                                                                                                                                                                                                                              gCCGTAGCGTATCGGGCGCTGCGTGACCA‑GTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTT‑‑AAAGAGAGCGTGGAAGCGATTCTCGGTGAAAC‑GTTGGATC‑TGCCAAAAGAGCTGGCAGAACGTGCTGATTTACCCTTGCTTTCACATAATCTGCCCGCCGATTTTGCTGCGTTGCGTAAATTGATGATGAATCATCAGTAAAATCTATTCATTATCTCAATCAgg  <  1:360378/249‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                      AACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGGGCTACACTTCGGAGCCGCACGCTGCCGTAGCTTATCGTGCGCTGCGTGATCA‑GTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTT‑‑AAAGAGAGCGTGGAAGCGATTCTCGGTGAAAC‑GTTGGATC‑TGCCAAAAGAGCTGGCAGAACGTGCTGATTTACCCTTGCTTTCACATAATCTGCCCGCCGATTTTGCTGCGTTGCGTAAATTGATGATGAATCATCAGTAAAATCTATTCATTATCTCAATCAGG  >  NC_000913/4559‑5044

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑