Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,617,616 T→C A98A (GCT→GCC)  deoC → deoxyribose‑phosphate aldolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,617,6160TC97.8% 140.3 / ‑4.7 45A98A (GCT→GCC) deoCdeoxyribose‑phosphate aldolase
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (28/16);  total (28/17)
Fisher's exact test for biased strand distribution p-value = 3.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.33e-01

GACCACCCTGAATGACGACGACACCGACGAGAAAGTGATCGCCCTGTGTCATCAGGCCAAAACTCCGG‑TCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACTCTGAAAGAGCAGGGCACCCCGGAAATCCGTATCGCTACGGTAACCAACTTCCCACACGGTAACGACGACATCGACATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCTGATGAAGTTGACGTTGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTTGACCTGGTGAAAGCCTGTAAAGAGGCTTGCGCGGC‑AGCGAATGTACTGCTG‑AAAGTGATCATCGAAACCGGCGAACTGAAAGACGAAGCGCTGATCCGTAAAGCGTCTGAAATCTCCATCAAAGCGGGTGCGGACTTCATCAAAACCTCTACCGGTAAAGTGGCTGTGAACGCGACG  >  NC_000913/4617373‑4617856
                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                  
gACCACCCTGAATGACGACGACACCGACGAGAAAGTAATTGCTCTGTGTCCTCAGGCCAAAACCCCGG‑TCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGCTTGCTCGCAAAACACTGACAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTACCGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCcgatgc                                                                                                                                                                                                                                               >  2:397292/1‑248 (MQ=255)
  ccaccCTGAATGACGACGACACCGACGAGAAAGTAATTGCTCTGTGTCATCAGGCCAAAACCCCGG‑TCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGtt                                                                                                                                                                                                                                           >  2:29222/1‑251 (MQ=255)
      ccTGAATGACGACGACACCGACGAGAAAGTAATTGCTCTGTGTCATCAGGCCAAAACCCCGG‑TCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACg                                                                                                                                                                                                                                       >  1:33925/1‑251 (MQ=255)
                   gacACCGACGAGAAAGTAATTGCTCTGTGTCATCAGGCCAAAACCCCGG‑TCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGt                                                                                                                                                                                                                           >  2:271430/1‑250 (MQ=255)
                                     attgctcTGTGTCATCAGGCCAAACCCCCGG‑TCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCggg                                                                                                                                                                                                        <  1:378491/245‑1 (MQ=255)
                                        gctcTGTGTCATCAGGCCAAA‑CCCCGG‑TCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGAGGTGTTCCCGTAACGCGCGCTGATGGCGGGGAAc                                                                                                                                                                                                    >  2:453024/4‑251 (MQ=255)
                                          cctggatcagcacgCCAAACCCCCGG‑CCGGAAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACg                                                                                                                                                                                                   <  2:195841/238‑1 (MQ=255)
                                              gtgacaaaGGCCAAAACCCCGGGTCGGAAAAACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAg                                                                                                                                                                                                 <  1:271430/243‑1 (MQ=255)
                                                 catcaGGCCAAAACCCCGG‑TCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAgagggg                                                                                                                                                                                            >  2:171057/1‑246 (MQ=255)
                                                        ccAAAACCCCGG‑TCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGtttt                                                                                                                                                                                     >  1:169116/1‑251 (MQ=255)
                                                          aaaaCCCCGG‑TCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGCCATCGAAATCGCGCTGGCAGAAACCCGTGCGGCCATCGCCTCCGGTGCCGATGAAGTTGACGTGGTGTTCCCGTCCCGCGCGCTGATGGCGGGTAACGAGCCGGGTGGTTTTgt                                                                                                                                                                                   >  2:222495/1‑250 (MQ=255)
                                                          aaaaCCCCGG‑TCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGGACCGGGCAGGGTGGGTTTgt                                                                                                                                                                                   >  2:140474/1‑250 (MQ=255)
                                                          aaaaCCCCGG‑TCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGGAACGAGCGGGGTGGTTTTg                                                                                                                                                                                    >  1:290030/1‑250 (MQ=255)
                                                            aaccccGG‑TCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTCTCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGGAAAGAGCAGGGTGGGTTTGAcc                                                                                                                                                                                 >  2:354587/5‑251 (MQ=255)
                                                               ccccggtaggaaaaaCCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATGGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTTGACCTg                                                                                                                                                                               <  1:287274/237‑1 (MQ=255)
                                                               cccGG‑TCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTTGACCTgg                                                                                                                                                                              <  1:446261/250‑1 (MQ=255)
                                                                 cGG‑TCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGGAGGTTGGTTTTTACCCGGtt                                                                                                                                                                            >  2:88785/1‑250 (MQ=255)
                                                                              ccgccgCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTTGTACTGGTGAAAGCCTGTaa                                                                                                                                                                 >  1:265177/1‑250 (MQ=255)
                                                                                 ccgcTATCTGTATCTATCCGCGCTTTATCCCGAATGCTCGCAAAACACTGAAAGAGCAGTGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTTGACCTGGTGAAAGCCTGTAAagag                                                                                                                                                             <  1:313577/251‑1 (MQ=255)
                                                                                  cgcTATCGGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGATCTCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGCAATCGCGCTGACAGAAACCCGTGCGGCAATCGCCTACGGTGCCGCTGAAGTTGACGTGGGGTGCCCGTAGCGCGCGCTGATGGCGGGTAACGAGCAGGGTGGGTTTGCCCCGGTGaagacctataaagggg                                                                                                                                                            >  2:202718/1‑237 (MQ=255)
                                                                                   gcTATCTGTATCTATCCTCGCTTTATCCCGATGGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGTCAGCAACCCGTGCGGAAATCGCCTACGGTGCCGATGTAGTTGACGTGGTGTTCCCGGACCGCGCGCTGTTGTCGGGTAACGAGCAGGTTGGTTTTGGCCCGGTGAAAGCCTTTAACGAGGc                                                                                                                                                           >  1:495040/1‑251 (MQ=255)
                                                                                             tctatcCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAGCGGGCAGGTTGGTTTTGACCTGGCGAAAGCCTGTAAAGGGGCTTgc                                                                                                                                                       >  1:470480/1‑245 (MQ=255)
                                                                                             tctatcCTCGCTTTAGCCCGATTTCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTGCCCGTACCGCGCGCTGAGGGCGGGTAACGAGCAGGTTGGTTTTGACCTGGTGAAAGCCTGTAAAGAGGCTTgc                                                                                                                                                       <  2:470480/245‑1 (MQ=255)
                                                                                                      gCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGCTGGTTTTGAACTGGTGAAAGCCTGGAAAGAGGCTTGCGCGGC‑CGCGAATGt                                                                                                                                        >  1:44433/1‑250 (MQ=255)
                                                                                                                                    aaGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGGTTTGACCTGGTGGAAGCCTGTAAAGAGGCTTGCGCGGC‑AGCGAATGTACTGCTG‑AAAGTGATCATCGAAACCGGCGa                                                                                                         >  1:73164/1‑250 (MQ=255)
                                                                                                                                     agagCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTTTACCTGGTGAAAGCCTGTAAAGAGGCTTGCGCGGC‑AGCGCATGTACTGCTG‑AAAGTGATCATCGAAACCGGCGAAc                                                                                                       >  1:503050/1‑251 (MQ=255)
                                                                                                                                      gagCAGGGCACCCCGGAAATCCGTATTGGTACGGTAACCACCCTCCCCCACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTTGACCTGGTGAAAGCCTGTAAAGAGGCTTGCGCGGC‑AGCGAATGTACTGCTG‑AAAGTGATCATCGAAACCGGCGAACt                                                                                                      <  2:153668/251‑1 (MQ=255)
                                                                                                                                             gCACCCCGGAAATCCGTATTGCTACGGTAAACAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTTGACCTGGTGAAAGCCTGTAAAGAGGCTTGCGCGGC‑AGCGGATGTAATGCTG‑AAAGAGATAATCGGAACCGGCGAACAGAAAGAc                                                                                               >  1:456359/1‑251 (MQ=255)
                                                                                                                                             gCACCCCGGAAATCCGTATCGCTACGGTAACCAACTTCCCACACGGTAACGACGACATCGACATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCTGATGAAGTTGACGTTGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTTGACCTGGTGAAAGCCTGTAAAGAGGCTTGCGCGGC‑AGCGAATGTACTGCTG‑aa                                                                                                                              <  1:252442/220‑1 (MQ=255)
                                                                                                                                                   cGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTTGACCTGGTGAAAGCCTGTAAAGAGGCTTGCGCGGC‑AGCGAATGTACTGCTG‑AAAGTGATCATCGAAACCGGCGAACTGAAAGACGAAgcg                                                                                         <  2:70633/251‑1 (MQ=255)
                                                                                                                                                        aTCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTTGACCTGGTGAAAGCCTGTAAAGAGGCTTGCGCGGC‑AGCGAATGTACTGCTG‑AAAGTGATCATCGAAACCGGCGAACTGAAAGACGAAGCGCTGAt                                                                                    <  1:225161/251‑1 (MQ=255)
                                                                                                                                                          ccGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGGTGGGTTTGACCTGGTGAAAGCCTGTAAAGAGGCTTGCGGGGG‑AGCGAATGTACTGCTG‑GAAGGGATCATCGAAACCGGCGAACTGAAAGACGAAAGGCTGAtac                                                                                  >  1:58728/1‑249 (MQ=255)
                                                                                                                                                             tattgCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGGAAAGAGCAGGTTGGTTTTGACATGGTGAAAGCCTGTAAAGAGGCTTGCGCGGC‑AGCGGATGTACTGCTGGAAAG‑GATCATCGAAACCGGCGGACAGAAAGACGaaaaccagatccgt                                                                                >  1:270101/5‑238 (MQ=255)
                                                                                                                                                              attgCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTTGACCTGGTGAAAGCCTGTAAAGAGGCTTGCGCGGC‑AGCGAATGTACTGCTG‑AAAGTGATCATCGAAACCGGCGAACTGAAAGACGAAGCGCTGATCCGTa                                                                               <  1:504424/247‑1 (MQ=255)
                                                                                                                                                                    aCGGTAACCAACGTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAATCCCGTGCGGCAATCGCCTACGGGGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTCTTGACCTGGTGAAAGCCTGTAAAGAGGCTTGCGCGGC‑AGCGAATGTACTGCTG‑AAAGTGATCAGCGAAACCGGCGAACTGAAAGACGAAGCGCTGATCCGTAAAGCg                                                                          <  2:265177/249‑1 (MQ=255)
                                                                                                                                                                         aaCCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTTGACCTGGTGAAAGCCTGTAAAGAGGCTTGCGCGGC‑AGCGGCAGTAATGCGG‑AAAGGGATACTACAAACCGGCGAACTGACAGACGGAgcggcgatacgttaaaggtcctga                                                                    >  2:203069/1‑229 (MQ=255)
                                                                                                                                                                                      cacGGTAACGGTGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTGGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGGTTTGACCTGGTGAAAGCCTGTAAAGAGGCTTGTGCGGC‑AGCGAATGTACTGCTG‑AAAGTGATCATCGAAACCGGCGAACTGAAAGACGAAGCGCTGATCCGTAAAGCGTCTGAAATCTCCATCAAAGc                                                      <  2:500770/251‑1 (MQ=255)
                                                                                                                                                                                      cacGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCCATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTTGACCTGGTGAAAGCCTGTAAAGAGGCTTGCGCGGC‑AGCGAATGTACTGCTG‑AAAGTGAACATCGTAACCGGCGAACTGAAAGACGAAGCGCTGATCCGTAAAGCGTCTGGAATCTCCATCAAAGc                                                      >  1:181160/1‑251 (MQ=255)
                                                                                                                                                                                          gTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTTGACCTGGTGAAAGCCTGTAAAGAGGCTTGCGCGGG‑AGCGAATGTACTGCTG‑AAAATGATCATCGAAACCGGCGAACTGAAAGAAGAAAAGCCGATCCGGTAAGCGTATGAAAACTCCATCAAAACgggg                                                  >  1:406432/1‑250 (MQ=255)
                                                                                                                                                                                                            ccgcgCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTTGACCTGGTGAAAGCCTGTAAAGAGGCTTGCGCGGC‑AGCGAATGTACTGCTG‑AAAGTGATCATCGAAACCGGCGAACTGAAAGACGAAGCGCTGATCCGTAAAGCGTCTGAAATCTCCATCAAAGCGGGTGCGGACTTCATCAAAAcc                                <  1:86634/250‑1 (MQ=255)
                                                                                                                                                                                                                  tGGCAGAAACCCGTGCGGCAATCGCCTGCGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGGTGGGTTTGAACTGGTGAAAGCCTGTAAAGAGGCTTGCGAGGCGACGGAAAG‑ACTGATG‑TAAGAGAACATAGAAACCGGCGAACTGAAAGACGAAGCGCTGATCCGGAAAGCGTCTGAAATCTCCATCAAAGCAGGTGCGGGCTTCATCAAAAACTCTAcc                          >  1:500013/1‑251 (MQ=255)
                                                                                                                                                                                                                  tGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGGTGGTTTTGACCTGGTGAAAGCCTGTAAAGAGGCTTGCGCGGC‑AGCGAATGTACTGCTG‑AAAGAGATCATCGAAACCGGAGAACTGAAAGAAGCAGAGCTGATCCGTAAAACGTCTGTAATCTCCATCAAAGCGGGTGCGGACTTCATCAAAACCTCAAcc                          >  1:204976/1‑251 (MQ=255)
                                                                                                                                                                                                                   ggCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTTGACCTGGTGAAAGCCTGTAAAGAGGCTTGCGCGGC‑AGCGAATGTACTGCTG‑AAAGTGATCATCGAAACCGGCGAACTGAAAGACGAAGCGCTGATCCGTAAAGCGTCTGAAATCTCCATCAAAGCGGGTGCGGACTTCATCAAAACCTCTAcc                          <  1:388693/250‑1 (MQ=255)
                                                                                                                                                                                                                                        cGCCTACGGTGCCGATGAAGGTGACGGTGTGTGCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGGTTGGACCTGGTGAAAGCCTGTAAAGAGGCTTGCGCTGC‑AGCGAATGTACTGCTG‑AAAGTGATCATCGAAACCGGCGTACTGAAAGACGAAGCGCTGATCCGTAAAGCGTCTGAAAGCTCCATCAAAGCTGGTGCGGACTTCATCAAATCCTCTACCGGTAAAGGGGCTGTGAAcgcg     <  2:169379/250‑1 (MQ=255)
                                                                                                                                                                                                                                         gCCTACGGTGCCGATGAAGTTGACGAGGTGTTCCCGTACCGCGCGCTGATGGCGGGGAACGAGCCGGTTGGTTTTGACCTGGTGAAAGCCTGTAAAGAGGCTTGCGCGGG‑AGAGAATGTAATGCTG‑AAAGTGATCATTGAGAACGGCGAAATGAAAAACGAAGCGCTGGTCCGTAAAGCGTCTGAAATCTCCATCAAAACGGGGGCGGACTTCATAAAAAACTCTACCGGTAAAGTGGAtgtggacccga    >  1:421554/1‑243 (MQ=255)
                                                                                                                                                                                                                                          ccTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTTGACCTGGTGAAAGCCTGTAAAGAGGCTTGCGCGGC‑AGCGAATGTACTGCTG‑AAAGTGATCATCGAAACCGGCGAACTGAAAGACGAAGCGCTGATCCGTAAAACGTCTGAAATCTCCATCAAAGCGGGGGCGGACTTCATCAAAACCTCTCCCGGTAAAGTGGCTGTTAACGCGACg  >  1:495874/1‑251 (MQ=255)
                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                  
GACCACCCTGAATGACGACGACACCGACGAGAAAGTGATCGCCCTGTGTCATCAGGCCAAAACTCCGG‑TCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACTCTGAAAGAGCAGGGCACCCCGGAAATCCGTATCGCTACGGTAACCAACTTCCCACACGGTAACGACGACATCGACATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCTGATGAAGTTGACGTTGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTTGACCTGGTGAAAGCCTGTAAAGAGGCTTGCGCGGC‑AGCGAATGTACTGCTG‑AAAGTGATCATCGAAACCGGCGAACTGAAAGACGAAGCGCTGATCCGTAAAGCGTCTGAAATCTCCATCAAAGCGGGTGCGGACTTCATCAAAACCTCTACCGGTAAAGTGGCTGTGAACGCGACG  >  NC_000913/4617373‑4617856

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑