Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,617,574 C→A D84E (GAC→GAA)  deoC → deoxyribose‑phosphate aldolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,617,5740CA97.9% 144.6 / ‑3.6 47D84E (GAC→GAA) deoCdeoxyribose‑phosphate aldolase
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base A (31/15);  total (31/16)
Fisher's exact test for biased strand distribution p-value = 3.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.35e-01

TGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTGATCGCCCTGTG‑TCATCAGGCCAAAACTCCGG‑TCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACTCTGAAAGAGCAGGGCACCCCGGAAATCCGTATCGCTACGGTAACCAACTTCCCACACGGTAACGACGACATCGACATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCTGATGAAGTTGACGTTGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTTGACCTGGTGAAAGCCTGTAAAGAGGCTTGCGCGGCAGCGAATGTACTGCTG‑AAAGTGATCATCGAAACCGGCGAACTGAAAGACGAAGCGCTGATCCGTAAAGCGTCTGAAATCTCCATCAAAGCGGGT  >  NC_000913/4617328‑4617808
                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                           
ggATCTAAAAGCAAGCAGCCTGCGTGCACTAAAATTGATGGACCTGCCCCCCCTGAATGACGACGACACCGAGGAAAAAGTAATTGCTCTGTG‑TCATCAGGCAAAACCCCCGG‑TCGGAAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGAATGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGacatcgaaatcg                                                                                                                                                                                                                                         <  1:9279/250‑1 (MQ=255)
        aagcaagcAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTAATTGCTCTGTG‑TCATCAGGCCAAAACCCCGG‑TCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGc                                                                                                                                                                                                                                  >  2:388693/1‑250 (MQ=255)
            aggcacccggCGTGCACTAAAATTGATGGACCGGACCACCCTAAATGACGACGACCCCGACGAAAAAGTAATTGCTCGGTT‑TCATCAGGCCAAACCCCCGT‑TCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGaaa                                                                                                                                                                                                                             <  1:300934/242‑1 (MQ=255)
                 cccggCGTGCACTGAAAGTGATGGACCGGCCCCCCCTAAAGGACGACGACCCCGAGGAGAATGTAATTGTT‑TGTGGTCATCGGGCCAAACCCCCGG‑TCGGCAATCCCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGt                                                                                                                                                                                                                        <  2:106792/247‑1 (MQ=255)
                      cGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTAATTGCTCTGTG‑TCATCAGGCCAAAACCCCGG‑TCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGCCATCGAAATCGCGCTGGCAGAAACCCGTGCGGc                                                                                                                                                                                                                   >  2:235767/1‑251 (MQ=255)
                      cGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTAATTGCTCTGTG‑TCATCAGGCCAAAACCCCGG‑TCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGc                                                                                                                                                                                                                   >  2:86634/1‑251 (MQ=255)
                         gCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTAATTGCTCTGTG‑TCATCAGGCCAAAACCCCGG‑TCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAAt                                                                                                                                                                                                                >  1:52627/1‑251 (MQ=255)
                                    gATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTAATTGCTCTGTG‑TCATCAGGCCAAAACCCCGG‑TCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTg                                                                                                                                                                                                     >  1:195841/1‑251 (MQ=255)
                                    gATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTAATTGCTCTGTG‑TCATCAGGCCAAAACCCCGG‑TCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTg                                                                                                                                                                                                     >  1:190287/1‑251 (MQ=255)
                                             gACCACCCTGAATGACGACGACACCGACGAGAAAGTAATTGCTCTGTG‑TCCTCAGGCCAAAACCCCGG‑TCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGCTTGCTCGCAAAACACTGACAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTACCGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCcgatgc                                                                                                                                                                                              >  2:397292/1‑248 (MQ=255)
                                               ccaccCTGAATGACGACGACACCGACGAGAAAGTAATTGCTCTGTG‑TCATCAGGCCAAAACCCCGG‑TCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGtt                                                                                                                                                                                          >  2:29222/1‑251 (MQ=255)
                                                   ccTGAATGACGACGACACCGACGAGAAAGTAATTGCTCTGTG‑TCATCAGGCCAAAACCCCGG‑TCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACg                                                                                                                                                                                      >  1:33925/1‑251 (MQ=255)
                                                                gacACCGACGAGAAAGTAATTGCTCTGTG‑TCATCAGGCCAAAACCCCGG‑TCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGt                                                                                                                                                                          >  2:271430/1‑250 (MQ=255)
                                                                                  attgctcTGTG‑TCATCAGGCCAAACCCCCGG‑TCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCggg                                                                                                                                                       <  1:378491/245‑1 (MQ=255)
                                                                                     gctcTGTG‑TCATCAGGCCAAA‑CCCCGG‑TCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGAGGTGTTCCCGTAACGCGCGCTGATGGCGGGGAAc                                                                                                                                                   >  2:453024/4‑251 (MQ=255)
                                                                                        cctggatcagcacgCCAAACCCCCGG‑CCGGAAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACg                                                                                                                                                  <  2:195841/238‑1 (MQ=255)
                                                                                            gtgacaaaGGCCAAAACCCCGGGTCGGAAAAACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAg                                                                                                                                                <  1:271430/243‑1 (MQ=255)
                                                                                               catcaGGCCAAAACCCCGG‑TCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAgagggg                                                                                                                                           >  2:171057/1‑246 (MQ=255)
                                                                                                      ccAAAACCCCGG‑TCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGtttt                                                                                                                                    >  1:169116/1‑251 (MQ=255)
                                                                                                        aaaaCCCCGG‑TCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGCCATCGAAATCGCGCTGGCAGAAACCCGTGCGGCCATCGCCTCCGGTGCCGATGAAGTTGACGTGGTGTTCCCGTCCCGCGCGCTGATGGCGGGTAACGAGCCGGGTGGTTTTgt                                                                                                                                  >  2:222495/1‑250 (MQ=255)
                                                                                                        aaaaCCCCGG‑TCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGGACCGGGCAGGGTGGGTTTgt                                                                                                                                  >  2:140474/1‑250 (MQ=255)
                                                                                                        aaaaCCCCGG‑TCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGGAACGAGCGGGGTGGTTTTg                                                                                                                                   >  1:290030/1‑250 (MQ=255)
                                                                                                          aaccccGG‑TCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTCTCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGGAAAGAGCAGGGTGGGTTTGAcc                                                                                                                                >  2:354587/5‑251 (MQ=255)
                                                                                                             ccccggtaggaaaaaCCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATGGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTTGACCTg                                                                                                                              <  1:287274/237‑1 (MQ=255)
                                                                                                             cccGG‑TCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTTGACCTgg                                                                                                                             <  1:446261/250‑1 (MQ=255)
                                                                                                               cGG‑TCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGGAGGTTGGTTTTTACCCGGtt                                                                                                                           >  2:88785/1‑250 (MQ=255)
                                                                                                                            ccgccgCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTTGTACTGGTGAAAGCCTGTaa                                                                                                                >  1:265177/1‑250 (MQ=255)
                                                                                                                               ccgcTATCTGTATCTATCCGCGCTTTATCCCGAATGCTCGCAAAACACTGAAAGAGCAGTGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTTGACCTGGTGAAAGCCTGTAAagag                                                                                                            <  1:313577/251‑1 (MQ=255)
                                                                                                                                cgcTATCGGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGATCTCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGCAATCGCGCTGACAGAAACCCGTGCGGCAATCGCCTACGGTGCCGCTGAAGTTGACGTGGGGTGCCCGTAGCGCGCGCTGATGGCGGGTAACGAGCAGGGTGGGTTTGCCCCGGTGaagacctataaagggg                                                                                                           >  2:202718/1‑237 (MQ=255)
                                                                                                                                 gcTATCTGTATCTATCCTCGCTTTATCCCGATGGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGTCAGCAACCCGTGCGGAAATCGCCTACGGTGCCGATGTAGTTGACGTGGTGTTCCCGGACCGCGCGCTGTTGTCGGGTAACGAGCAGGTTGGTTTTGGCCCGGTGAAAGCCTTTAACGAGGc                                                                                                          >  1:495040/1‑251 (MQ=255)
                                                                                                                                           tctatcCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAGCGGGCAGGTTGGTTTTGACCTGGCGAAAGCCTGTAAAGGGGCTTgc                                                                                                      >  1:470480/1‑245 (MQ=255)
                                                                                                                                           tctatcCTCGCTTTAGCCCGATTTCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTGCCCGTACCGCGCGCTGAGGGCGGGTAACGAGCAGGTTGGTTTTGACCTGGTGAAAGCCTGTAAAGAGGCTTgc                                                                                                      <  2:470480/245‑1 (MQ=255)
                                                                                                                                                    gCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGCTGGTTTTGAACTGGTGAAAGCCTGGAAAGAGGCTTGCGCGGCCGCGAATGt                                                                                        >  1:44433/1‑250 (MQ=255)
                                                                                                                                                                                  aaGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGGTTTGACCTGGTGGAAGCCTGTAAAGAGGCTTGCGCGGCAGCGAATGTACTGCTG‑AAAGTGATCATCGAAACCGGCGa                                                         >  1:73164/1‑250 (MQ=255)
                                                                                                                                                                                   agagCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTTTACCTGGTGAAAGCCTGTAAAGAGGCTTGCGCGGCAGCGCATGTACTGCTG‑AAAGTGATCATCGAAACCGGCGAAc                                                       >  1:503050/1‑251 (MQ=255)
                                                                                                                                                                                    gagCAGGGCACCCCGGAAATCCGTATTGGTACGGTAACCACCCTCCCCCACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTTGACCTGGTGAAAGCCTGTAAAGAGGCTTGCGCGGCAGCGAATGTACTGCTG‑AAAGTGATCATCGAAACCGGCGAACt                                                      <  2:153668/251‑1 (MQ=255)
                                                                                                                                                                                           gCACCCCGGAAATCCGTATTGCTACGGTAAACAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTTGACCTGGTGAAAGCCTGTAAAGAGGCTTGCGCGGCAGCGGATGTAATGCTG‑AAAGAGATAATCGGAACCGGCGAACAGAAAGAc                                               >  1:456359/1‑251 (MQ=255)
                                                                                                                                                                                           gCACCCCGGAAATCCGTATCGCTACGGTAACCAACTTCCCACACGGTAACGACGACATCGACATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCTGATGAAGTTGACGTTGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTTGACCTGGTGAAAGCCTGTAAAGAGGCTTGCGCGGCAGCGAATGTACTGCTG‑aa                                                                              <  1:252442/220‑1 (MQ=255)
                                                                                                                                                                                                 cGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTTGACCTGGTGAAAGCCTGTAAAGAGGCTTGCGCGGCAGCGAATGTACTGCTG‑AAAGTGATCATCGAAACCGGCGAACTGAAAGACGAAgcg                                         <  2:70633/251‑1 (MQ=255)
                                                                                                                                                                                                      aTCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTTGACCTGGTGAAAGCCTGTAAAGAGGCTTGCGCGGCAGCGAATGTACTGCTG‑AAAGTGATCATCGAAACCGGCGAACTGAAAGACGAAGCGCTGAt                                    <  1:225161/251‑1 (MQ=255)
                                                                                                                                                                                                        ccGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGGTGGGTTTGACCTGGTGAAAGCCTGTAAAGAGGCTTGCGGGGGAGCGAATGTACTGCTG‑GAAGGGATCATCGAAACCGGCGAACTGAAAGACGAAAGGCTGAtac                                  >  1:58728/1‑249 (MQ=255)
                                                                                                                                                                                                           tattgCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGGAAAGAGCAGGTTGGTTTTGACATGGTGAAAGCCTGTAAAGAGGCTTGCGCGGCAGCGGATGTACTGCTGGAAAG‑GATCATCGAAACCGGCGGACAGAAAGACGaaaaccagatccgt                                >  1:270101/5‑238 (MQ=255)
                                                                                                                                                                                                            attgCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTTGACCTGGTGAAAGCCTGTAAAGAGGCTTGCGCGGCAGCGAATGTACTGCTG‑AAAGTGATCATCGAAACCGGCGAACTGAAAGACGAAGCGCTGATCCGTa                               <  1:504424/247‑1 (MQ=255)
                                                                                                                                                                                                                  aCGGTAACCAACGTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAATCCCGTGCGGCAATCGCCTACGGGGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTCTTGACCTGGTGAAAGCCTGTAAAGAGGCTTGCGCGGCAGCGAATGTACTGCTG‑AAAGTGATCAGCGAAACCGGCGAACTGAAAGACGAAGCGCTGATCCGTAAAGCg                          <  2:265177/249‑1 (MQ=255)
                                                                                                                                                                                                                       aaCCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTTGACCTGGTGAAAGCCTGTAAAGAGGCTTGCGCGGCAGCGGCAGTAATGCGG‑AAAGGGATACTACAAACCGGCGAACTGACAGACGGAgcggcgatacgttaaaggtcctga                    >  2:203069/1‑229 (MQ=255)
                                                                                                                                                                                                                                    cacGGTAACGGTGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTGGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGGTTTGACCTGGTGAAAGCCTGTAAAGAGGCTTGTGCGGCAGCGAATGTACTGCTG‑AAAGTGATCATCGAAACCGGCGAACTGAAAGACGAAGCGCTGATCCGTAAAGCGTCTGAAATCTCCATCAAAGc      <  2:500770/251‑1 (MQ=255)
                                                                                                                                                                                                                                    cacGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCCATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTTGACCTGGTGAAAGCCTGTAAAGAGGCTTGCGCGGCAGCGAATGTACTGCTG‑AAAGTGAACATCGTAACCGGCGAACTGAAAGACGAAGCGCTGATCCGTAAAGCGTCTGGAATCTCCATCAAAGc      >  1:181160/1‑251 (MQ=255)
                                                                                                                                                                                                                                        gTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTTGACCTGGTGAAAGCCTGTAAAGAGGCTTGCGCGGGAGCGAATGTACTGCTG‑AAAATGATCATCGAAACCGGCGAACTGAAAGAAGAAAAGCCGATCCGGTAAGCGTATGAAAACTCCATCAAAACgggg  >  1:406432/1‑250 (MQ=255)
                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                           
TGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTGATCGCCCTGTG‑TCATCAGGCCAAAACTCCGG‑TCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACTCTGAAAGAGCAGGGCACCCCGGAAATCCGTATCGCTACGGTAACCAACTTCCCACACGGTAACGACGACATCGACATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCTGATGAAGTTGACGTTGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTTGACCTGGTGAAAGCCTGTAAAGAGGCTTGCGCGGCAGCGAATGTACTGCTG‑AAAGTGATCATCGAAACCGGCGAACTGAAAGACGAAGCGCTGATCCGTAAAGCGTCTGAAATCTCCATCAAAGCGGGT  >  NC_000913/4617328‑4617808

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑