Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,617,565 C→T D81D (GAC→GAT)  deoC → deoxyribose‑phosphate aldolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,617,5650CT97.9% 145.8 / ‑4.4 48D81D (GAC→GAT) deoCdeoxyribose‑phosphate aldolase
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base T (31/16);  total (31/17)
Fisher's exact test for biased strand distribution p-value = 3.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.27e-01

AAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTGATCGCCCTGTG‑TCATCAGGCCAAAACTCCGG‑TCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACTCTGAAAGAGCAGGGCACCCCGGAAATCCGTATCGCTACGGTAACCAACTTCCCACACGGTAACGACGACATCGACATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCTGATGAAGTTGACGTTGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTTGACCTGGTGAAAGCCTGTAAAGAGGCTTGCGCGGCAGCGAATGTACTGCTG‑AAAGTGATCATCGAAACCGGCGAACTGAAAGACGAAGCGCTGATCCGTAAAGCGTCTGAAATCTCCATCAAAGCGGGT  >  NC_000913/4617321‑4617808
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                    
aaatgaCTGATCTGAAAGCAAGCAGCCGGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTAATTGCTCTGTG‑TCATCAGGCCAAAACCCCGG‑TCGGCCATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCCAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCACCTTCCCACCCGGTAACGCtgacatc                                                                                                                                                                                                                                                >  2:325566/1‑251 (MQ=255)
     attGATCTGAAAGCAAGCAGCCTGCGTGCACTGAATTTTATGGCCCTGACCCCCCTGAATGACGACGACACCGACGAGAAAGTAATTGCTCTGTG‑TCATCCGGCCAAACCCCCGG‑TCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGTTAACGAtgacatcgaaat                                                                                                                                                                                                                                           <  1:152945/249‑4 (MQ=255)
       ggATCTAAAAGCAAGCAGCCTGCGTGCACTAAAATTGATGGACCTGCCCCCCCTGAATGACGACGACACCGAGGAAAAAGTAATTGCTCTGTG‑TCATCAGGCAAAACCCCCGG‑TCGGAAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGAATGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGacatcgaaatcg                                                                                                                                                                                                                                         <  1:9279/250‑1 (MQ=255)
               aagcaagcAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTAATTGCTCTGTG‑TCATCAGGCCAAAACCCCGG‑TCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGc                                                                                                                                                                                                                                  >  2:388693/1‑250 (MQ=255)
                   aggcacccggCGTGCACTAAAATTGATGGACCGGACCACCCTAAATGACGACGACCCCGACGAAAAAGTAATTGCTCGGTT‑TCATCAGGCCAAACCCCCGT‑TCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGaaa                                                                                                                                                                                                                             <  1:300934/242‑1 (MQ=255)
                        cccggCGTGCACTGAAAGTGATGGACCGGCCCCCCCTAAAGGACGACGACCCCGAGGAGAATGTAATTGTT‑TGTGGTCATCGGGCCAAACCCCCGG‑TCGGCAATCCCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGt                                                                                                                                                                                                                        <  2:106792/247‑1 (MQ=255)
                             cGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTAATTGCTCTGTG‑TCATCAGGCCAAAACCCCGG‑TCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGCCATCGAAATCGCGCTGGCAGAAACCCGTGCGGc                                                                                                                                                                                                                   >  2:235767/1‑251 (MQ=255)
                             cGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTAATTGCTCTGTG‑TCATCAGGCCAAAACCCCGG‑TCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGc                                                                                                                                                                                                                   >  2:86634/1‑251 (MQ=255)
                                gCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTAATTGCTCTGTG‑TCATCAGGCCAAAACCCCGG‑TCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAAt                                                                                                                                                                                                                >  1:52627/1‑251 (MQ=255)
                                           gATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTAATTGCTCTGTG‑TCATCAGGCCAAAACCCCGG‑TCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTg                                                                                                                                                                                                     >  1:190287/1‑251 (MQ=255)
                                           gATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTAATTGCTCTGTG‑TCATCAGGCCAAAACCCCGG‑TCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTg                                                                                                                                                                                                     >  1:195841/1‑251 (MQ=255)
                                                    gACCACCCTGAATGACGACGACACCGACGAGAAAGTAATTGCTCTGTG‑TCCTCAGGCCAAAACCCCGG‑TCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGCTTGCTCGCAAAACACTGACAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTACCGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCcgatgc                                                                                                                                                                                              >  2:397292/1‑248 (MQ=255)
                                                      ccaccCTGAATGACGACGACACCGACGAGAAAGTAATTGCTCTGTG‑TCATCAGGCCAAAACCCCGG‑TCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGtt                                                                                                                                                                                          >  2:29222/1‑251 (MQ=255)
                                                          ccTGAATGACGACGACACCGACGAGAAAGTAATTGCTCTGTG‑TCATCAGGCCAAAACCCCGG‑TCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACg                                                                                                                                                                                      >  1:33925/1‑251 (MQ=255)
                                                                       gacACCGACGAGAAAGTAATTGCTCTGTG‑TCATCAGGCCAAAACCCCGG‑TCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGt                                                                                                                                                                          >  2:271430/1‑250 (MQ=255)
                                                                                         attgctcTGTG‑TCATCAGGCCAAACCCCCGG‑TCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCggg                                                                                                                                                       <  1:378491/245‑1 (MQ=255)
                                                                                            gctcTGTG‑TCATCAGGCCAAA‑CCCCGG‑TCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGAGGTGTTCCCGTAACGCGCGCTGATGGCGGGGAAc                                                                                                                                                   >  2:453024/4‑251 (MQ=255)
                                                                                               cctggatcagcacgCCAAACCCCCGG‑CCGGAAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACg                                                                                                                                                  <  2:195841/238‑1 (MQ=255)
                                                                                                   gtgacaaaGGCCAAAACCCCGGGTCGGAAAAACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAg                                                                                                                                                <  1:271430/243‑1 (MQ=255)
                                                                                                      catcaGGCCAAAACCCCGG‑TCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAgagggg                                                                                                                                           >  2:171057/1‑246 (MQ=255)
                                                                                                             ccAAAACCCCGG‑TCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGtttt                                                                                                                                    >  1:169116/1‑251 (MQ=255)
                                                                                                               aaaaCCCCGG‑TCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGCCATCGAAATCGCGCTGGCAGAAACCCGTGCGGCCATCGCCTCCGGTGCCGATGAAGTTGACGTGGTGTTCCCGTCCCGCGCGCTGATGGCGGGTAACGAGCCGGGTGGTTTTgt                                                                                                                                  >  2:222495/1‑250 (MQ=255)
                                                                                                               aaaaCCCCGG‑TCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGGACCGGGCAGGGTGGGTTTgt                                                                                                                                  >  2:140474/1‑250 (MQ=255)
                                                                                                               aaaaCCCCGG‑TCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGGAACGAGCGGGGTGGTTTTg                                                                                                                                   >  1:290030/1‑250 (MQ=255)
                                                                                                                 aaccccGG‑TCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTCTCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGGAAAGAGCAGGGTGGGTTTGAcc                                                                                                                                >  2:354587/5‑251 (MQ=255)
                                                                                                                    ccccggtaggaaaaaCCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATGGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTTGACCTg                                                                                                                              <  1:287274/237‑1 (MQ=255)
                                                                                                                    cccGG‑TCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTTGACCTgg                                                                                                                             <  1:446261/250‑1 (MQ=255)
                                                                                                                      cGG‑TCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGGAGGTTGGTTTTTACCCGGtt                                                                                                                           >  2:88785/1‑250 (MQ=255)
                                                                                                                                   ccgccgCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTTGTACTGGTGAAAGCCTGTaa                                                                                                                >  1:265177/1‑250 (MQ=255)
                                                                                                                                      ccgcTATCTGTATCTATCCGCGCTTTATCCCGAATGCTCGCAAAACACTGAAAGAGCAGTGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTTGACCTGGTGAAAGCCTGTAAagag                                                                                                            <  1:313577/251‑1 (MQ=255)
                                                                                                                                       cgcTATCGGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGATCTCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGCAATCGCGCTGACAGAAACCCGTGCGGCAATCGCCTACGGTGCCGCTGAAGTTGACGTGGGGTGCCCGTAGCGCGCGCTGATGGCGGGTAACGAGCAGGGTGGGTTTGCCCCGGTGaagacctataaagggg                                                                                                           >  2:202718/1‑237 (MQ=255)
                                                                                                                                        gcTATCTGTATCTATCCTCGCTTTATCCCGATGGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGTCAGCAACCCGTGCGGAAATCGCCTACGGTGCCGATGTAGTTGACGTGGTGTTCCCGGACCGCGCGCTGTTGTCGGGTAACGAGCAGGTTGGTTTTGGCCCGGTGAAAGCCTTTAACGAGGc                                                                                                          >  1:495040/1‑251 (MQ=255)
                                                                                                                                                  tctatcCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAGCGGGCAGGTTGGTTTTGACCTGGCGAAAGCCTGTAAAGGGGCTTgc                                                                                                      >  1:470480/1‑245 (MQ=255)
                                                                                                                                                  tctatcCTCGCTTTAGCCCGATTTCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTGCCCGTACCGCGCGCTGAGGGCGGGTAACGAGCAGGTTGGTTTTGACCTGGTGAAAGCCTGTAAAGAGGCTTgc                                                                                                      <  2:470480/245‑1 (MQ=255)
                                                                                                                                                           gCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGCTGGTTTTGAACTGGTGAAAGCCTGGAAAGAGGCTTGCGCGGCCGCGAATGt                                                                                        >  1:44433/1‑250 (MQ=255)
                                                                                                                                                                                         aaGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGGTTTGACCTGGTGGAAGCCTGTAAAGAGGCTTGCGCGGCAGCGAATGTACTGCTG‑AAAGTGATCATCGAAACCGGCGa                                                         >  1:73164/1‑250 (MQ=255)
                                                                                                                                                                                          agagCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTTTACCTGGTGAAAGCCTGTAAAGAGGCTTGCGCGGCAGCGCATGTACTGCTG‑AAAGTGATCATCGAAACCGGCGAAc                                                       >  1:503050/1‑251 (MQ=255)
                                                                                                                                                                                           gagCAGGGCACCCCGGAAATCCGTATTGGTACGGTAACCACCCTCCCCCACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTTGACCTGGTGAAAGCCTGTAAAGAGGCTTGCGCGGCAGCGAATGTACTGCTG‑AAAGTGATCATCGAAACCGGCGAACt                                                      <  2:153668/251‑1 (MQ=255)
                                                                                                                                                                                                  gCACCCCGGAAATCCGTATTGCTACGGTAAACAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTTGACCTGGTGAAAGCCTGTAAAGAGGCTTGCGCGGCAGCGGATGTAATGCTG‑AAAGAGATAATCGGAACCGGCGAACAGAAAGAc                                               >  1:456359/1‑251 (MQ=255)
                                                                                                                                                                                                  gCACCCCGGAAATCCGTATCGCTACGGTAACCAACTTCCCACACGGTAACGACGACATCGACATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCTGATGAAGTTGACGTTGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTTGACCTGGTGAAAGCCTGTAAAGAGGCTTGCGCGGCAGCGAATGTACTGCTG‑aa                                                                              <  1:252442/220‑1 (MQ=255)
                                                                                                                                                                                                        cGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTTGACCTGGTGAAAGCCTGTAAAGAGGCTTGCGCGGCAGCGAATGTACTGCTG‑AAAGTGATCATCGAAACCGGCGAACTGAAAGACGAAgcg                                         <  2:70633/251‑1 (MQ=255)
                                                                                                                                                                                                             aTCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTTGACCTGGTGAAAGCCTGTAAAGAGGCTTGCGCGGCAGCGAATGTACTGCTG‑AAAGTGATCATCGAAACCGGCGAACTGAAAGACGAAGCGCTGAt                                    <  1:225161/251‑1 (MQ=255)
                                                                                                                                                                                                               ccGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGGTGGGTTTGACCTGGTGAAAGCCTGTAAAGAGGCTTGCGGGGGAGCGAATGTACTGCTG‑GAAGGGATCATCGAAACCGGCGAACTGAAAGACGAAAGGCTGAtac                                  >  1:58728/1‑249 (MQ=255)
                                                                                                                                                                                                                  tattgCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGGAAAGAGCAGGTTGGTTTTGACATGGTGAAAGCCTGTAAAGAGGCTTGCGCGGCAGCGGATGTACTGCTGGAAAG‑GATCATCGAAACCGGCGGACAGAAAGACGaaaaccagatccgt                                >  1:270101/5‑238 (MQ=255)
                                                                                                                                                                                                                   attgCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTTGACCTGGTGAAAGCCTGTAAAGAGGCTTGCGCGGCAGCGAATGTACTGCTG‑AAAGTGATCATCGAAACCGGCGAACTGAAAGACGAAGCGCTGATCCGTa                               <  1:504424/247‑1 (MQ=255)
                                                                                                                                                                                                                         aCGGTAACCAACGTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAATCCCGTGCGGCAATCGCCTACGGGGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTCTTGACCTGGTGAAAGCCTGTAAAGAGGCTTGCGCGGCAGCGAATGTACTGCTG‑AAAGTGATCAGCGAAACCGGCGAACTGAAAGACGAAGCGCTGATCCGTAAAGCg                          <  2:265177/249‑1 (MQ=255)
                                                                                                                                                                                                                              aaCCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTTGACCTGGTGAAAGCCTGTAAAGAGGCTTGCGCGGCAGCGGCAGTAATGCGG‑AAAGGGATACTACAAACCGGCGAACTGACAGACGGAgcggcgatacgttaaaggtcctga                    >  2:203069/1‑229 (MQ=255)
                                                                                                                                                                                                                                           cacGGTAACGGTGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTGGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGGTTTGACCTGGTGAAAGCCTGTAAAGAGGCTTGTGCGGCAGCGAATGTACTGCTG‑AAAGTGATCATCGAAACCGGCGAACTGAAAGACGAAGCGCTGATCCGTAAAGCGTCTGAAATCTCCATCAAAGc      <  2:500770/251‑1 (MQ=255)
                                                                                                                                                                                                                                           cacGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCCATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTTGACCTGGTGAAAGCCTGTAAAGAGGCTTGCGCGGCAGCGAATGTACTGCTG‑AAAGTGAACATCGTAACCGGCGAACTGAAAGACGAAGCGCTGATCCGTAAAGCGTCTGGAATCTCCATCAAAGc      >  1:181160/1‑251 (MQ=255)
                                                                                                                                                                                                                                               gTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTTGACCTGGTGAAAGCCTGTAAAGAGGCTTGCGCGGGAGCGAATGTACTGCTG‑AAAATGATCATCGAAACCGGCGAACTGAAAGAAGAAAAGCCGATCCGGTAAGCGTATGAAAACTCCATCAAAACgggg  >  1:406432/1‑250 (MQ=255)
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                    
AAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTGATCGCCCTGTG‑TCATCAGGCCAAAACTCCGG‑TCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACTCTGAAAGAGCAGGGCACCCCGGAAATCCGTATCGCTACGGTAACCAACTTCCCACACGGTAACGACGACATCGACATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCTGATGAAGTTGACGTTGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTTGACCTGGTGAAAGCCTGTAAAGAGGCTTGCGCGGCAGCGAATGTACTGCTG‑AAAGTGATCATCGAAACCGGCGAACTGAAAGACGAAGCGCTGATCCGTAAAGCGTCTGAAATCTCCATCAAAGCGGGT  >  NC_000913/4617321‑4617808

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑