Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,617,532	C→T	I70I (ATC→ATT)	deoC →	deoxyribose‑phosphate aldolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,617,532	0	C	T	97.9%	147.0 / ‑4.2	47	I70I (ATC→ATT)	deoC	deoxyribose‑phosphate aldolase
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base T (32/14);  total (32/15)
Fisher's exact test for biased strand distribution p-value = 3.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.55e-01

AAGGTGAATTTTATTGGCGACAAGCCAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTGATCGCCCTGTG‑TCATCAGGCCAAAACTCCGG‑TCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACTCTGAAAGAGCAGGGCACCCCGGAAATCCGTATCGCTACGGTAACCAACTTCCCACACGGTAACGACGACATCGACATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCTGATGAAGTTGACGTTGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTTGACCTGGTGAAAGCCTGTAAAGAGGCTTGCGCGGCAGCGAATGTACTGCTGAAAGTGATCATCGAAACCGGCGAACTGAAAGACGAAGCGCTGATCC  >  NC_000913/4617286‑4617776                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                     aaGGTGGATTTTATTGGCGACAAGCCAGGAGAATGCCATGACTGATCTGAAAGAAAGCAGCCTGCGTGCACTGAAATTGATGGACCAGACCACCCTGAATGACGACGACCCCGCAGAGAAAGTAATTGATCTGTG‑TCATCAGGCCAAAACCCCGG‑ACGGCACTACCGCCGCTATCTGTCTCTCACCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACACCGGAAATCCGTATTGCt                                                                                                                                                                                                                                                   >  2:196459/1‑250 (MQ=255)               tGGCGACAAGCCAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTAATTGCTCTGTG‑TCATCAGGCCAAAACCCCGG‑TCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTc                                                                                                                                                                                                                                    >  1:106792/1‑251 (MQ=255)                   gACAAGCCAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTAATTGCTCTGTG‑TCATCAGGCCAAAACCCCGG‑TCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTaa                                                                                                                                                                                                                                            >  1:236908/1‑239 (MQ=255)                        gCCAGGCGACTGCAATGACTGCTCTGAAAGCCAGCAGCCTGCGCGCACTGAAATTGCTGGACCTGACCACCCTGAATGACGACGACACCGACGAGAACGTAATTGCTCTGTG‑TCATCAGGCCAAAACCCCGG‑TCGGCACTACCGCCGCTATCTGTATCTATCATCGCTTTATCCCGATTGCTCGCAAACAACTGAAAGAGCAGGGCGCCCCGGAAATCCGTATTGCTACGGTACCCAACTTCCCGCACGGt                                                                                                                                                                                                                           >  2:233540/1‑251 (MQ=255)                                    aaatgaCTGATCTGAAAGCAAGCAGCCGGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTAATTGCTCTGTG‑TCATCAGGCCAAAACCCCGG‑TCGGCCATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCCAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCACCTTCCCACCCGGTAACGCtgacatc                                                                                                                                                                                                               >  2:325566/1‑251 (MQ=255)                                         attGATCTGAAAGCAAGCAGCCTGCGTGCACTGAATTTTATGGCCCTGACCCCCCTGAATGACGACGACACCGACGAGAAAGTAATTGCTCTGTG‑TCATCCGGCCAAACCCCCGG‑TCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGTTAACGAtgacatcgaaat                                                                                                                                                                                                          <  1:152945/249‑4 (MQ=255)                                           ggATCTAAAAGCAAGCAGCCTGCGTGCACTAAAATTGATGGACCTGCCCCCCCTGAATGACGACGACACCGAGGAAAAAGTAATTGCTCTGTG‑TCATCAGGCAAAACCCCCGG‑TCGGAAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGAATGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGacatcgaaatcg                                                                                                                                                                                                        <  1:9279/250‑1 (MQ=255)                                                   aagcaagcAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTAATTGCTCTGTG‑TCATCAGGCCAAAACCCCGG‑TCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGc                                                                                                                                                                                                 >  2:388693/1‑250 (MQ=255)                                                       aggcacccggCGTGCACTAAAATTGATGGACCGGACCACCCTAAATGACGACGACCCCGACGAAAAAGTAATTGCTCGGTT‑TCATCAGGCCAAACCCCCGT‑TCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGaaa                                                                                                                                                                                            <  1:300934/242‑1 (MQ=255)                                                            cccggCGTGCACTGAAAGTGATGGACCGGCCCCCCCTAAAGGACGACGACCCCGAGGAGAATGTAATTGTT‑TGTGGTCATCGGGCCAAACCCCCGG‑TCGGCAATCCCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGt                                                                                                                                                                                       <  2:106792/247‑1 (MQ=255)                                                                 cGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTAATTGCTCTGTG‑TCATCAGGCCAAAACCCCGG‑TCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGCCATCGAAATCGCGCTGGCAGAAACCCGTGCGGc                                                                                                                                                                                  >  2:235767/1‑251 (MQ=255)                                                                 cGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTAATTGCTCTGTG‑TCATCAGGCCAAAACCCCGG‑TCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGc                                                                                                                                                                                  >  2:86634/1‑251 (MQ=255)                                                                    gCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTAATTGCTCTGTG‑TCATCAGGCCAAAACCCCGG‑TCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAAt                                                                                                                                                                               >  1:52627/1‑251 (MQ=255)                                                                               gATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTAATTGCTCTGTG‑TCATCAGGCCAAAACCCCGG‑TCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTg                                                                                                                                                                    >  1:190287/1‑251 (MQ=255)                                                                               gATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTAATTGCTCTGTG‑TCATCAGGCCAAAACCCCGG‑TCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTg                                                                                                                                                                    >  1:195841/1‑251 (MQ=255)                                                                                        gACCACCCTGAATGACGACGACACCGACGAGAAAGTAATTGCTCTGTG‑TCCTCAGGCCAAAACCCCGG‑TCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGCTTGCTCGCAAAACACTGACAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTACCGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCcgatgc                                                                                                                                                             >  2:397292/1‑248 (MQ=255)                                                                                          ccaccCTGAATGACGACGACACCGACGAGAAAGTAATTGCTCTGTG‑TCATCAGGCCAAAACCCCGG‑TCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGtt                                                                                                                                                         >  2:29222/1‑251 (MQ=255)                                                                                              ccTGAATGACGACGACACCGACGAGAAAGTAATTGCTCTGTG‑TCATCAGGCCAAAACCCCGG‑TCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACg                                                                                                                                                     >  1:33925/1‑251 (MQ=255)                                                                                                           gacACCGACGAGAAAGTAATTGCTCTGTG‑TCATCAGGCCAAAACCCCGG‑TCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGt                                                                                                                                         >  2:271430/1‑250 (MQ=255)                                                                                                                             attgctcTGTG‑TCATCAGGCCAAACCCCCGG‑TCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCggg                                                                                                                      <  1:378491/245‑1 (MQ=255)                                                                                                                                gctcTGTG‑TCATCAGGCCAAA‑CCCCGG‑TCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGAGGTGTTCCCGTAACGCGCGCTGATGGCGGGGAAc                                                                                                                  >  2:453024/4‑251 (MQ=255)                                                                                                                                   cctggatcagcacgCCAAACCCCCGG‑CCGGAAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACg                                                                                                                 <  2:195841/238‑1 (MQ=255)                                                                                                                                       gtgacaaaGGCCAAAACCCCGGGTCGGAAAAACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAg                                                                                                               <  1:271430/243‑1 (MQ=255)                                                                                                                                          catcaGGCCAAAACCCCGG‑TCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAgagggg                                                                                                          >  2:171057/1‑246 (MQ=255)                                                                                                                                                 ccAAAACCCCGG‑TCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGtttt                                                                                                   >  1:169116/1‑251 (MQ=255)                                                                                                                                                   aaaaCCCCGG‑TCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGCCATCGAAATCGCGCTGGCAGAAACCCGTGCGGCCATCGCCTCCGGTGCCGATGAAGTTGACGTGGTGTTCCCGTCCCGCGCGCTGATGGCGGGTAACGAGCCGGGTGGTTTTgt                                                                                                 >  2:222495/1‑250 (MQ=255)                                                                                                                                                   aaaaCCCCGG‑TCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGGACCGGGCAGGGTGGGTTTgt                                                                                                 >  2:140474/1‑250 (MQ=255)                                                                                                                                                   aaaaCCCCGG‑TCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGGAACGAGCGGGGTGGTTTTg                                                                                                  >  1:290030/1‑250 (MQ=255)                                                                                                                                                     aaccccGG‑TCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTCTCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGGAAAGAGCAGGGTGGGTTTGAcc                                                                                               >  2:354587/5‑251 (MQ=255)                                                                                                                                                        ccccggtaggaaaaaCCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATGGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTTGACCTg                                                                                             <  1:287274/237‑1 (MQ=255)                                                                                                                                                        cccGG‑TCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTTGACCTgg                                                                                            <  1:446261/250‑1 (MQ=255)                                                                                                                                                          cGG‑TCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGGAGGTTGGTTTTTACCCGGtt                                                                                          >  2:88785/1‑250 (MQ=255)                                                                                                                                                                       ccgccgCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTTGTACTGGTGAAAGCCTGTaa                                                                               >  1:265177/1‑250 (MQ=255)                                                                                                                                                                          ccgcTATCTGTATCTATCCGCGCTTTATCCCGAATGCTCGCAAAACACTGAAAGAGCAGTGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTTGACCTGGTGAAAGCCTGTAAagag                                                                           <  1:313577/251‑1 (MQ=255)                                                                                                                                                                           cgcTATCGGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGATCTCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGCAATCGCGCTGACAGAAACCCGTGCGGCAATCGCCTACGGTGCCGCTGAAGTTGACGTGGGGTGCCCGTAGCGCGCGCTGATGGCGGGTAACGAGCAGGGTGGGTTTGCCCCGGTGaagacctataaagggg                                                                          >  2:202718/1‑237 (MQ=255)                                                                                                                                                                            gcTATCTGTATCTATCCTCGCTTTATCCCGATGGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGTCAGCAACCCGTGCGGAAATCGCCTACGGTGCCGATGTAGTTGACGTGGTGTTCCCGGACCGCGCGCTGTTGTCGGGTAACGAGCAGGTTGGTTTTGGCCCGGTGAAAGCCTTTAACGAGGc                                                                         >  1:495040/1‑251 (MQ=255)                                                                                                                                                                                      tctatcCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAGCGGGCAGGTTGGTTTTGACCTGGCGAAAGCCTGTAAAGGGGCTTgc                                                                     >  1:470480/1‑245 (MQ=255)                                                                                                                                                                                      tctatcCTCGCTTTAGCCCGATTTCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTGCCCGTACCGCGCGCTGAGGGCGGGTAACGAGCAGGTTGGTTTTGACCTGGTGAAAGCCTGTAAAGAGGCTTgc                                                                     <  2:470480/245‑1 (MQ=255)                                                                                                                                                                                               gCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGCTGGTTTTGAACTGGTGAAAGCCTGGAAAGAGGCTTGCGCGGCCGCGAATGt                                                       >  1:44433/1‑250 (MQ=255)                                                                                                                                                                                                                             aaGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGGTTTGACCTGGTGGAAGCCTGTAAAGAGGCTTGCGCGGCAGCGAATGTACTGCTGAAAGTGATCATCGAAACCGGCGa                         >  1:73164/1‑250 (MQ=255)                                                                                                                                                                                                                              agagCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTTTACCTGGTGAAAGCCTGTAAAGAGGCTTGCGCGGCAGCGCATGTACTGCTGAAAGTGATCATCGAAACCGGCGAAc                       >  1:503050/1‑251 (MQ=255)                                                                                                                                                                                                                               gagCAGGGCACCCCGGAAATCCGTATTGGTACGGTAACCACCCTCCCCCACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTTGACCTGGTGAAAGCCTGTAAAGAGGCTTGCGCGGCAGCGAATGTACTGCTGAAAGTGATCATCGAAACCGGCGAACt                      <  2:153668/251‑1 (MQ=255)                                                                                                                                                                                                                                      gCACCCCGGAAATCCGTATTGCTACGGTAAACAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTTGACCTGGTGAAAGCCTGTAAAGAGGCTTGCGCGGCAGCGGATGTAATGCTGAAAGAGATAATCGGAACCGGCGAACAGAAAGAc               >  1:456359/1‑251 (MQ=255)                                                                                                                                                                                                                                      gCACCCCGGAAATCCGTATCGCTACGGTAACCAACTTCCCACACGGTAACGACGACATCGACATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCTGATGAAGTTGACGTTGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTTGACCTGGTGAAAGCCTGTAAAGAGGCTTGCGCGGCAGCGAATGTACTGCTGaa                                              <  1:252442/220‑1 (MQ=255)                                                                                                                                                                                                                                            cGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTTGACCTGGTGAAAGCCTGTAAAGAGGCTTGCGCGGCAGCGAATGTACTGCTGAAAGTGATCATCGAAACCGGCGAACTGAAAGACGAAgcg         <  2:70633/251‑1 (MQ=255)                                                                                                                                                                                                                                                 aTCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTTGACCTGGTGAAAGCCTGTAAAGAGGCTTGCGCGGCAGCGAATGTACTGCTGAAAGTGATCATCGAAACCGGCGAACTGAAAGACGAAGCGCTGAt    <  1:225161/251‑1 (MQ=255)                                                                                                                                                                                                                                                   ccGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGGTGGGTTTGACCTGGTGAAAGCCTGTAAAGAGGCTTGCGGGGGAGCGAATGTACTGCTGGAAGGGATCATCGAAACCGGCGAACTGAAAGACGAAAGGCTGAtac  >  1:58728/1‑249 (MQ=255)                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                     AAGGTGAATTTTATTGGCGACAAGCCAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTGATCGCCCTGTG‑TCATCAGGCCAAAACTCCGG‑TCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACTCTGAAAGAGCAGGGCACCCCGGAAATCCGTATCGCTACGGTAACCAACTTCCCACACGGTAACGACGACATCGACATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCTGATGAAGTTGACGTTGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTTGACCTGGTGAAAGCCTGTAAAGAGGCTTGCGCGGCAGCGAATGTACTGCTGAAAGTGATCATCGAAACCGGCGAACTGAAAGACGAAGCGCTGATCC  >  NC_000913/4617286‑4617776

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑