Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,617,499	T→A	T59T (ACT→ACA)	deoC →	deoxyribose‑phosphate aldolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,617,499	0	T	A	100.0%	133.7 / NA	43	T59T (ACT→ACA)	deoC	deoxyribose‑phosphate aldolase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (31/12);  total (31/12)

AGATGTTAGAATACTAACAAACTCGCAAGGTGAATTTTATTGGCGACAAGCCAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTGATCGCCCTGTG‑TCATCAGGCCAAAACTCCGG‑TCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACTCTGAAAGAGCAGGGCACCCCGGAAATCCGTATCGCTACGGTAACCAACTTCCCACACGGTAACGACGACATCGACATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCTGATGAAGTTGACGTTGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTTGACCTGGTGAAAGCCTGTAAAGAGGCTTGCGCGGCAGCGAATGT  >  NC_000913/4617260‑4617723                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                 aGATGTTAGAATACTAACAAACTCGCAAGGTGAATTTTATTGGCGACAAGCCAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTAATTGCTCTGTG‑TCATCAGGCCAAAACCCCGG‑TCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAACCACTGAAAGAGc                                                                                                                                                                                                                        >  2:504424/1‑250 (MQ=255)                aaCAAACTCGCAAGGTGAATTTTATTGGCGCCAAGCCAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTAATTGCTCTGTG‑TCATCAGGCCAAAACCCCGG‑TCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAAt                                                                                                                                                                                                        >  2:287274/1‑251 (MQ=255)                           aaGGTGGATTTTATTGGCGACAAGCCAGGAGAATGCCATGACTGATCTGAAAGAAAGCAGCCTGCGTGCACTGAAATTGATGGACCAGACCACCCTGAATGACGACGACCCCGCAGAGAAAGTAATTGATCTGTG‑TCATCAGGCCAAAACCCCGG‑ACGGCACTACCGCCGCTATCTGTCTCTCACCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACACCGGAAATCCGTATTGCt                                                                                                                                                                                              >  2:196459/1‑250 (MQ=255)                                         tGGCGACAAGCCAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTAATTGCTCTGTG‑TCATCAGGCCAAAACCCCGG‑TCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTc                                                                                                                                                                               >  1:106792/1‑251 (MQ=255)                                             gACAAGCCAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTAATTGCTCTGTG‑TCATCAGGCCAAAACCCCGG‑TCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTaa                                                                                                                                                                                       >  1:236908/1‑239 (MQ=255)                                                  gCCAGGCGACTGCAATGACTGCTCTGAAAGCCAGCAGCCTGCGCGCACTGAAATTGCTGGACCTGACCACCCTGAATGACGACGACACCGACGAGAACGTAATTGCTCTGTG‑TCATCAGGCCAAAACCCCGG‑TCGGCACTACCGCCGCTATCTGTATCTATCATCGCTTTATCCCGATTGCTCGCAAACAACTGAAAGAGCAGGGCGCCCCGGAAATCCGTATTGCTACGGTACCCAACTTCCCGCACGGt                                                                                                                                                                      >  2:233540/1‑251 (MQ=255)                                                              aaatgaCTGATCTGAAAGCAAGCAGCCGGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTAATTGCTCTGTG‑TCATCAGGCCAAAACCCCGG‑TCGGCCATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCCAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCACCTTCCCACCCGGTAACGCtgacatc                                                                                                                                                          >  2:325566/1‑251 (MQ=255)                                                                   attGATCTGAAAGCAAGCAGCCTGCGTGCACTGAATTTTATGGCCCTGACCCCCCTGAATGACGACGACACCGACGAGAAAGTAATTGCTCTGTG‑TCATCCGGCCAAACCCCCGG‑TCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGTTAACGAtgacatcgaaat                                                                                                                                                     <  1:152945/249‑4 (MQ=255)                                                                     ggATCTAAAAGCAAGCAGCCTGCGTGCACTAAAATTGATGGACCTGCCCCCCCTGAATGACGACGACACCGAGGAAAAAGTAATTGCTCTGTG‑TCATCAGGCAAAACCCCCGG‑TCGGAAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGAATGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGacatcgaaatcg                                                                                                                                                   <  1:9279/250‑1 (MQ=255)                                                                             aagcaagcAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTAATTGCTCTGTG‑TCATCAGGCCAAAACCCCGG‑TCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGc                                                                                                                                            >  2:388693/1‑250 (MQ=255)                                                                                 aggcacccggCGTGCACTAAAATTGATGGACCGGACCACCCTAAATGACGACGACCCCGACGAAAAAGTAATTGCTCGGTT‑TCATCAGGCCAAACCCCCGT‑TCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGaaa                                                                                                                                       <  1:300934/242‑1 (MQ=255)                                                                                    cagcCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTAATTGCTCTGTG‑TCATCAGGCCAAAACCCCGG‑TCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAg                                                                                                                                                                                                                      >  2:207519/1‑169 (MQ=255)                                                                                    cagcCTGCGTGCACTAAAATTGATGGCCCTGACCCCCCTGAATGACGACGACCCCGACGAGAAAGTAATTGCTCGTTG‑TCATCAGGCCAAACCCCCGG‑TCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAg                                                                                                                                                                                                                      <  1:207519/169‑1 (MQ=255)                                                                                      cccggCGTGCACTGAAAGTGATGGACCGGCCCCCCCTAAAGGACGACGACCCCGAGGAGAATGTAATTGTT‑TGTGGTCATCGGGCCAAACCCCCGG‑TCGGCAATCCCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGt                                                                                                                                  <  2:106792/247‑1 (MQ=255)                                                                                           cGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTAATTGCTCTGTG‑TCATCAGGCCAAAACCCCGG‑TCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGCCATCGAAATCGCGCTGGCAGAAACCCGTGCGGc                                                                                                                             >  2:235767/1‑251 (MQ=255)                                                                                           cGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTAATTGCTCTGTG‑TCATCAGGCCAAAACCCCGG‑TCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGc                                                                                                                             >  2:86634/1‑251 (MQ=255)                                                                                              gCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTAATTGCTCTGTG‑TCATCAGGCCAAAACCCCGG‑TCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAAt                                                                                                                          >  1:52627/1‑251 (MQ=255)                                                                                                         gATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTAATTGCTCTGTG‑TCATCAGGCCAAAACCCCGG‑TCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTg                                                                                                               >  1:195841/1‑251 (MQ=255)                                                                                                         gATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTAATTGCTCTGTG‑TCATCAGGCCAAAACCCCGG‑TCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTg                                                                                                               >  1:190287/1‑251 (MQ=255)                                                                                                                  gACCACCCTGAATGACGACGACACCGACGAGAAAGTAATTGCTCTGTG‑TCCTCAGGCCAAAACCCCGG‑TCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGCTTGCTCGCAAAACACTGACAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTACCGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCcgatgc                                                                                                        >  2:397292/1‑248 (MQ=255)                                                                                                                    ccaccCTGAATGACGACGACACCGACGAGAAAGTAATTGCTCTGTG‑TCATCAGGCCAAAACCCCGG‑TCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGtt                                                                                                    >  2:29222/1‑251 (MQ=255)                                                                                                                        ccTGAATGACGACGACACCGACGAGAAAGTAATTGCTCTGTG‑TCATCAGGCCAAAACCCCGG‑TCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACg                                                                                                >  1:33925/1‑251 (MQ=255)                                                                                                                                     gacACCGACGAGAAAGTAATTGCTCTGTG‑TCATCAGGCCAAAACCCCGG‑TCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGt                                                                                    >  2:271430/1‑250 (MQ=255)                                                                                                                                                       attgctcTGTG‑TCATCAGGCCAAACCCCCGG‑TCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCggg                                                                 <  1:378491/245‑1 (MQ=255)                                                                                                                                                          gctcTGTG‑TCATCAGGCCAAA‑CCCCGG‑TCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGAGGTGTTCCCGTAACGCGCGCTGATGGCGGGGAAc                                                             >  2:453024/4‑251 (MQ=255)                                                                                                                                                             cctggatcagcacgCCAAACCCCCGG‑CCGGAAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACg                                                            <  2:195841/238‑1 (MQ=255)                                                                                                                                                                 gtgacaaaGGCCAAAACCCCGGGTCGGAAAAACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAg                                                          <  1:271430/243‑1 (MQ=255)                                                                                                                                                                    catcaGGCCAAAACCCCGG‑TCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAgagggg                                                     >  2:171057/1‑246 (MQ=255)                                                                                                                                                                           ccAAAACCCCGG‑TCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGtttt                                              >  1:169116/1‑251 (MQ=255)                                                                                                                                                                             aaaaCCCCGG‑TCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGCCATCGAAATCGCGCTGGCAGAAACCCGTGCGGCCATCGCCTCCGGTGCCGATGAAGTTGACGTGGTGTTCCCGTCCCGCGCGCTGATGGCGGGTAACGAGCCGGGTGGTTTTgt                                            >  2:222495/1‑250 (MQ=255)                                                                                                                                                                             aaaaCCCCGG‑TCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGGACCGGGCAGGGTGGGTTTgt                                            >  2:140474/1‑250 (MQ=255)                                                                                                                                                                             aaaaCCCCGG‑TCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGGAACGAGCGGGGTGGTTTTg                                             >  1:290030/1‑250 (MQ=255)                                                                                                                                                                               aaccccGG‑TCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTCTCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGGAAAGAGCAGGGTGGGTTTGAcc                                          >  2:354587/5‑251 (MQ=255)                                                                                                                                                                                  ccccggtaggaaaaaCCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATGGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTTGACCTg                                        <  1:287274/237‑1 (MQ=255)                                                                                                                                                                                  cccGG‑TCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTTGACCTgg                                       <  1:446261/250‑1 (MQ=255)                                                                                                                                                                                    cGG‑TCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGGAGGTTGGTTTTTACCCGGtt                                     >  2:88785/1‑250 (MQ=255)                                                                                                                                                                                                 ccgccgCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTTGTACTGGTGAAAGCCTGTaa                          >  1:265177/1‑250 (MQ=255)                                                                                                                                                                                                    ccgcTATCTGTATCTATCCGCGCTTTATCCCGAATGCTCGCAAAACACTGAAAGAGCAGTGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTTGACCTGGTGAAAGCCTGTAAagag                      <  1:313577/251‑1 (MQ=255)                                                                                                                                                                                                     cgcTATCGGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGATCTCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGCAATCGCGCTGACAGAAACCCGTGCGGCAATCGCCTACGGTGCCGCTGAAGTTGACGTGGGGTGCCCGTAGCGCGCGCTGATGGCGGGTAACGAGCAGGGTGGGTTTGCCCCGGTGaagacctataaagggg                     >  2:202718/1‑237 (MQ=255)                                                                                                                                                                                                      gcTATCTGTATCTATCCTCGCTTTATCCCGATGGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGTCAGCAACCCGTGCGGAAATCGCCTACGGTGCCGATGTAGTTGACGTGGTGTTCCCGGACCGCGCGCTGTTGTCGGGTAACGAGCAGGTTGGTTTTGGCCCGGTGAAAGCCTTTAACGAGGc                    >  1:495040/1‑251 (MQ=255)                                                                                                                                                                                                                tctatcCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAGCGGGCAGGTTGGTTTTGACCTGGCGAAAGCCTGTAAAGGGGCTTgc                >  1:470480/1‑245 (MQ=255)                                                                                                                                                                                                                tctatcCTCGCTTTAGCCCGATTTCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTGCCCGTACCGCGCGCTGAGGGCGGGTAACGAGCAGGTTGGTTTTGACCTGGTGAAAGCCTGTAAAGAGGCTTgc                <  2:470480/245‑1 (MQ=255)                                                                                                                                                                                                                         gCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGCTGGTTTTGAACTGGTGAAAGCCTGGAAAGAGGCTTGCGCGGCCGCGAATGt  >  1:44433/1‑250 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                 AGATGTTAGAATACTAACAAACTCGCAAGGTGAATTTTATTGGCGACAAGCCAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTGATCGCCCTGTG‑TCATCAGGCCAAAACTCCGG‑TCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACTCTGAAAGAGCAGGGCACCCCGGAAATCCGTATCGCTACGGTAACCAACTTCCCACACGGTAACGACGACATCGACATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCTGATGAAGTTGACGTTGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTTGACCTGGTGAAAGCCTGTAAAGAGGCTTGCGCGGCAGCGAATGT  >  NC_000913/4617260‑4617723

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑