Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,617,415	C→T	A31A (GCC→GCT)	deoC →	deoxyribose‑phosphate aldolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,617,415	0	C	T	100.0%	110.1 / NA	37	A31A (GCC→GCT)	deoC	deoxyribose‑phosphate aldolase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (25/12);  total (25/12)

ATTATTTGAACCAGATCGCATTACAGTGATGCAAACTTGTAAGTAGATTTCCTTAATTGTGATGTGTATCGAAGTGTGTTGCGGAGTAGATGTTAGAATACTAACAAACTCGCAAGGTGAATTTTATTGGCGACAAGCCAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTGATCGCCCTGTG‑TCATCAGGCCAAAACTCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACTCTGAAAGAGCAGGGCACCCCGGAAATCCGTATCGCTACGGTAACCAACTTCCCACACGGTAACGACGACATCGACATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCTGATGAAGTTGACGTTGTGTTCCCGT  >  NC_000913/4617173‑4617641                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                    attattTGAACCAGATCGCATTACAGTGATGCAAACTTGTAAGTAGATTTCCTTAATTGTGATGTATATCGAAGTGTGTTGCGGAGTAGATGTTAGAATACTAACAAACTCGCAAGGTGAATTTTATTGGCGACAAGCCAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTAATTGCTCTGTG‑tca                                                                                                                                                                                                                            <  2:284023/251‑1 (MQ=255)                      tACAGTGATGCAAACTTGTAAGTAGATTTCCTTAATTGTGATGTATATCGAAGTGTGTTGCGGAGTAGATGTTAGAATACTAACAAACTCGCAAGGTGAATTTTATTGGCGACAAGCCAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACCGCCGAGAAAGTAATTGCTCTGTG‑TCCTCAGGCCAAACCCCCGGTCgg                                                                                                                                                                                                       >  2:152945/1‑251 (MQ=255)                          gTGATGCAAACTTGTAAGTAGATTTCCTTAATTGTGATGTATATCGAAGTGTGTTGCGGAGTAGATGTTAGAATACTAACAAACTCGCAAGGTGAATTTTATTGGCGACAAGCCAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCCCCCTGAATGACGACGACACCGACGATAAAGTAATTGCTCTGTG‑TCATCAGGCCAAAACCCCGGTCGGCaag                                                                                                                                                                                                   <  1:174703/251‑2 (MQ=255)                             aTGCAAACTTGTAAGTAGATTTCCTTAATTTTGATGTATATCGAAGTGTGTTGCGGAGTAGATGTTAGAATACTAACAAACTCGCAAGGTGAATTTTATTGGCGACAAGCCAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTAATTGCTCTGTG‑TCATCAGGCCAAAACCCCGGTCGGCAATAcc                                                                                                                                                                                                <  2:385390/251‑1 (MQ=255)                                cAAACTTGTAAGTAGATTTCCTTAATTGTGATGTATATCGAAGTGTGTTGCGGAGTAGATGTTAGAATACTAACAAACTCGTAAGGTGAATTTTATTGGCGACAAGCCAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACAACCCTGATTGACGACGACACCGACGAGAAAGTAATTGCTCTGTG‑TAATAAGGCCAAAACCCCGGTCGGCAATAccgcc                                                                                                                                                                                             >  2:281002/1‑251 (MQ=255)                                                 ttCCTTAATTGTGATGTATATCGAAGTGTGTTGCGGAGTAGATGTTAGAATACTAACAAACTCGCAAGGTGAATTTTATTGGCGACAAGCCAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTAATTGCTCTGTG‑TCATCAGGCCAAAACCCCGGTCGGCAATACCGCCGCTATCTGTATATATcc                                                                                                                                                                            >  2:455910/1‑251 (MQ=255)                                                  tCCTTAATTGTGATGTATATCGAAGTGTGTTGCGGAGTAGATGTTAGAATACTAACAAACTCGCAAGGTGAATTTTATTGGCGACAAGCCAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGAACTGACCACCCTGAATGACTACGACACCGACGCGAAAGTAATTGCTCTGTG‑TCATCAGGCCAAAACCCCGGTCGGCAATACCGCCGCTATCTTTATCTATCCt                                                                                                                                                                           >  1:435031/1‑251 (MQ=255)                                                   cctaaattgggatttatATCGAGGTGTGTTGCGGAGTAGATTTTAGAATACTAAAAAACTCGAAAGGTAAATTTTATGGGCGACAAGCCAGGAGAATAAAATGTCTAATCTGAAAGCAAGCACCCGGCGTGCACTGAAATTGAGGGCCCTACCCCCCCTGAATGACGACGCCCCCGACGAAAAAGAATTTGCTCTGTT‑TCTTCAGCCCAAACCCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCt                                                                                                                                                                           <  2:205971/234‑1 (MQ=255)                                                   ccTTAATTGTGATGTATATCGAAGTGTGTTGCGGAGTAGATGTTAAAATACTAACAAACTCGAAGGGTGATTTTTATTGGCGACAAGCCAGGAGAATGAAATGATGGATCGGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGCCCGCACCACCCTGAATGACGACGACCCCGACGAGAAAGTAATTGCTCTGTG‑TCATCAGGCAAAACCCCCGGTCGTCAATACCGCCGCTATCTGTATCTATCCt                                                                                                                                                                           <  1:239559/250‑1 (MQ=255)                                                         ttGTGATGTATATCGAAGTGTGTTGCGGAGTAGATGTTAGAATACTAACAAACTCGCAAGGTGAATTTTATTGGCGACAAGCCAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTAATTGCTCTGTG‑TCATCAGGCCAAAACCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCttt                                                                                                                                                                     >  1:70633/1‑250 (MQ=255)                                                              aTGTATATCGAAGTGTGTTGCGGAGTAGATGTTAGAATACTAACAAACTCGCAAGGTGAATTTTATTGGCGACAAGCCAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTAATTGCTCTGTG‑TCATCAGGCCAAAACCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCg                                                                                                                                                               >  1:191879/1‑251 (MQ=255)                                                                       gAAGTGTGTTGCGGAGTAGATGTTAGAATACTAACAAACTCGCAAGGTGAATTTTATTGGCGACAAGCCAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTAATTGCTCTGTG‑TCATCAGGCCa                                                                                                                                                                                                                    <  1:247863/189‑1 (MQ=255)                                                                                        aGATGTTAGAATACTAACAAACTCGCAAGGTGAATTTTATTGGCGACAAGCCAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTAATTGCTCTGTG‑TCATCAGGCCAAAACCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAACCACTGAAAGAGc                                                                                                                                      >  2:504424/1‑250 (MQ=255)                                                                                                       aaCAAACTCGCAAGGTGAATTTTATTGGCGCCAAGCCAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTAATTGCTCTGTG‑TCATCAGGCCAAAACCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAAt                                                                                                                      >  2:287274/1‑251 (MQ=255)                                                                                                                  aaGGTGGATTTTATTGGCGACAAGCCAGGAGAATGCCATGACTGATCTGAAAGAAAGCAGCCTGCGTGCACTGAAATTGATGGACCAGACCACCCTGAATGACGACGACCCCGCAGAGAAAGTAATTGATCTGTG‑TCATCAGGCCAAAACCCCGGACGGCACTACCGCCGCTATCTGTCTCTCACCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACACCGGAAATCCGTATTGCt                                                                                                            >  2:196459/1‑250 (MQ=255)                                                                                                                    ggTGAATTTTATTGGCGACAAGCCAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTAATTGCTCTGTG‑TCATCAGGCCAAAACCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAAc                                                                                                                                                 >  2:135888/1‑211 (MQ=255)                                                                                                                    ggTGAATTTTATGGGCGACAAGCCAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGCCCCCCCTGAATGACGACGACACCGACGAGAAAGTAATTGCTCTGTT‑TCATCAGGCAAAACCCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAAc                                                                                                                                                 <  1:135888/211‑1 (MQ=255)                                                                                                                                tGGCGACAAGCCAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTAATTGCTCTGTG‑TCATCAGGCCAAAACCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTc                                                                                             >  1:106792/1‑251 (MQ=255)                                                                                                                                    gACAAGCCAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTAATTGCTCTGTG‑TCATCAGGCCAAAACCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTaa                                                                                                     >  1:236908/1‑239 (MQ=255)                                                                                                                                         gCCAGGCGACTGCAATGACTGCTCTGAAAGCCAGCAGCCTGCGCGCACTGAAATTGCTGGACCTGACCACCCTGAATGACGACGACACCGACGAGAACGTAATTGCTCTGTG‑TCATCAGGCCAAAACCCCGGTCGGCACTACCGCCGCTATCTGTATCTATCATCGCTTTATCCCGATTGCTCGCAAACAACTGAAAGAGCAGGGCGCCCCGGAAATCCGTATTGCTACGGTACCCAACTTCCCGCACGGt                                                                                    >  2:233540/1‑251 (MQ=255)                                                                                                                                                     aaatgaCTGATCTGAAAGCAAGCAGCCGGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTAATTGCTCTGTG‑TCATCAGGCCAAAACCCCGGTCGGCCATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCCAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCACCTTCCCACCCGGTAACGCtgacatc                                                                        >  2:325566/1‑251 (MQ=255)                                                                                                                                                          attGATCTGAAAGCAAGCAGCCTGCGTGCACTGAATTTTATGGCCCTGACCCCCCTGAATGACGACGACACCGACGAGAAAGTAATTGCTCTGTG‑TCATCCGGCCAAACCCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGTTAACGAtgacatcgaaat                                                                   <  1:152945/249‑4 (MQ=255)                                                                                                                                                            ggATCTAAAAGCAAGCAGCCTGCGTGCACTAAAATTGATGGACCTGCCCCCCCTGAATGACGACGACACCGAGGAAAAAGTAATTGCTCTGTG‑TCATCAGGCAAAACCCCCGGTCGGAAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGAATGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGacatcgaaatcg                                                                 <  1:9279/250‑1 (MQ=255)                                                                                                                                                                    aagcaagcAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTAATTGCTCTGTG‑TCATCAGGCCAAAACCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGc                                                          >  2:388693/1‑250 (MQ=255)                                                                                                                                                                        aggcacccggCGTGCACTAAAATTGATGGACCGGACCACCCTAAATGACGACGACCCCGACGAAAAAGTAATTGCTCGGTT‑TCATCAGGCCAAACCCCCGTTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGaaa                                                     <  1:300934/242‑1 (MQ=255)                                                                                                                                                                           cagcCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTAATTGCTCTGTG‑TCATCAGGCCAAAACCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAg                                                                                                                                    >  2:207519/1‑169 (MQ=255)                                                                                                                                                                           cagcCTGCGTGCACTAAAATTGATGGCCCTGACCCCCCTGAATGACGACGACCCCGACGAGAAAGTAATTGCTCGTTG‑TCATCAGGCCAAACCCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAg                                                                                                                                    <  1:207519/169‑1 (MQ=255)                                                                                                                                                                             cccggCGTGCACTGAAAGTGATGGACCGGCCCCCCCTAAAGGACGACGACCCCGAGGAGAATGTAATTGTT‑TGTGGTCATCGGGCCAAACCCCCGGTCGGCAATCCCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGt                                                <  2:106792/247‑1 (MQ=255)                                                                                                                                                                                  cGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTAATTGCTCTGTG‑TCATCAGGCCAAAACCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGCCATCGAAATCGCGCTGGCAGAAACCCGTGCGGc                                           >  2:235767/1‑251 (MQ=255)                                                                                                                                                                                  cGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTAATTGCTCTGTG‑TCATCAGGCCAAAACCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGc                                           >  2:86634/1‑251 (MQ=255)                                                                                                                                                                                     gCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTAATTGCTCTGTG‑TCATCAGGCCAAAACCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAAt                                        >  1:52627/1‑251 (MQ=255)                                                                                                                                                                                                gATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTAATTGCTCTGTG‑TCATCAGGCCAAAACCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTg                             >  1:195841/1‑251 (MQ=255)                                                                                                                                                                                                gATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTAATTGCTCTGTG‑TCATCAGGCCAAAACCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTg                             >  1:190287/1‑251 (MQ=255)                                                                                                                                                                                                         gACCACCCTGAATGACGACGACACCGACGAGAAAGTAATTGCTCTGTG‑TCCTCAGGCCAAAACCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGCTTGCTCGCAAAACACTGACAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTACCGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCcgatgc                      >  2:397292/1‑248 (MQ=255)                                                                                                                                                                                                           ccaccCTGAATGACGACGACACCGACGAGAAAGTAATTGCTCTGTG‑TCATCAGGCCAAAACCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGtt                  >  2:29222/1‑251 (MQ=255)                                                                                                                                                                                                               ccTGAATGACGACGACACCGACGAGAAAGTAATTGCTCTGTG‑TCATCAGGCCAAAACCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACg              >  1:33925/1‑251 (MQ=255)                                                                                                                                                                                                                            gacACCGACGAGAAAGTAATTGCTCTGTG‑TCATCAGGCCAAAACCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGt  >  2:271430/1‑250 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                    ATTATTTGAACCAGATCGCATTACAGTGATGCAAACTTGTAAGTAGATTTCCTTAATTGTGATGTGTATCGAAGTGTGTTGCGGAGTAGATGTTAGAATACTAACAAACTCGCAAGGTGAATTTTATTGGCGACAAGCCAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTGATCGCCCTGTG‑TCATCAGGCCAAAACTCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACTCTGAAAGAGCAGGGCACCCCGGAAATCCGTATCGCTACGGTAACCAACTTCCCACACGGTAACGACGACATCGACATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCTGATGAAGTTGACGTTGTGTTCCCGT  >  NC_000913/4617173‑4617641

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑