Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,617,053 G→C H5D (CAT→GAT)  yjjI ← DUF3029 domain‑containing protein YjjI

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,617,0530GC93.2% 192.2 / 5.2 60H5D (CAT→GAT) yjjIDUF3029 domain‑containing protein YjjI
Reads supporting (aligned to +/- strand):  ref base G (0/4);  new base C (19/36);  total (20/40)
Fisher's exact test for biased strand distribution p-value = 2.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.49e-01

AGCCATTCGGAACCGTTCGCCAGAAAACGGGCGTAATCGGGTAAGACATAGCGCGGTTTGTACGGCGCATGACCTTCAAACATATCGCAGATTACACCTTCATCCAGCGCGCGGCGGGCTTCGGCAGGAAGCTGTGGGTAAGGCAGATTGTTTTCTGCTTCCAGTGCCAGAAAATGGCGCTTCTGCTCCGGGCTAAGCACTGGGCTGGTGACAATTTGCTGGCAACGTTGTTGCAGTGCATTTTCATGAGAAGTGGGCATCTTCTTTTCCTTTTATGCCGAAGGTGATGCGCCATTGTAAGAAGTTTCGTGATGTTCACTTTGATCCTGATGCGTTTGCCACCACTGACGCATTCATTTGAAAGTGAATTATTTGAACCAGATCGCATTACAGTGATGCAAACTTGTAAGTAGATTTCCTTAATTGTGATGTGTATCGAAGTGTGTTGCGGAGTAGATGTTAGAATACTAACAAACTCGCAAGGTGAATTTTAT  >  NC_000913/4616806‑4617299
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                      
agcCATTCGGAACCGTTCGCCAGAAAACGGGCGTAATCGGGTAAGACATAGCGCGGTTTGTACGGCGCATGACCTTCAAACATATCGCAGATTACACCTTCATCCAGCGCGCGGCGGGCTTCGGCAGGAAGCTGTGGGTAAGGCAGATTGTTTTCTGCTTCCAGTGCCAGAAAATGGCGCTTCTGCTCCGGGCTAAGCACTGGGCTGGTGACAATTTGCTGGCAACGTTGTTGCAGTGCATTTTCATcaga                                                                                                                                                                                                                                                     <  1:217085/251‑1 (MQ=255)
      tCGGAACCGTTCGCCAGAAAACGGGCGTAATCGGGTAAGACATAGCGCGGTTTGTACGGCGCATGACCTTCAAACATATCGCAGATTACACCTTCATCCAGCGCGCGGCGGGCTTCGGCAGGAAGCTGTGGGTAAGGCAGATTGTTTTCTGCTTCCAGTGCCAGAAAATGGCGCTTCTGCTCCGGGCTAAGCACTGGGCTGGTGACAATTTGCTGGCAACGTTGTTGCAGTGCATTTTCATCAGAAGTgg                                                                                                                                                                                                                                                <  1:447682/250‑1 (MQ=255)
        ggAACCGTTCGCCAGAAAACGGGCGTAATCGGGTAAGACATAGCGCGGTTTGTACGGCGCATGACCTTCAAACATATCGCAGATTACACCTTCATCCAGCGCGCGGCGGGCTTCGGCAGGAAGCTGTGGGTAAGGCAGATTGTTTTCTGCTTCCAGTGCCAGAAAATGGCGCTTCTGCTCCGGGCTAAGCACTGGGCTGGTGACAATTTGCTGGCAACGTTGTTGCAGTGCATTTTCATCAGAAGTGGGCa                                                                                                                                                                                                                                             <  2:210600/251‑1 (MQ=255)
                 cGCCAGAAAACGGGCGTAATCGGGTAAGACATAGCGCGGTTTGTACGGCGCATGACCTTCAAACATATCGCAGATTACACCTTCATCCAGCGCGCGGCGGGCTTCGGCAGGAAGCTGTGGGTAAGGCAGATTGTTTTCTGCTTCCAGTGCCAGAAAATGGCGCTTCTGCTCCGGGCTAAGCACTGGGCTGGTGACAATTTGCTGGCAACGTTGTTGCAGTGCATTTTCATCAGAAGTGGGCATCTTCtttt                                                                                                                                                                                                                                    <  1:167952/251‑1 (MQ=255)
                 cGCCAGAAAACGGGCGTAATCGGGTAAGACATAGCGCGGTTTGTACGGCGCATGACCTTCAAACATATCGCAGATTACACCTTCATCCAGCGCGCGGCGGGCTTCGGCAGGAAGCTGTGGGTAAGGCAGATTGTTTTCTGCTTCCAGTGCCAGAAAATGGCGCTTCTGCTCCGGGCTAAGCACTGGGCTGGTGACAATTTGCTGGCAACGTTGTTGCAGTGCATTTTCATCAGAAGTGGGCATCTTCttt                                                                                                                                                                                                                                     <  1:355909/250‑1 (MQ=255)
                     aGAAAACGGGCGTAATCGGGTAAGACATAGCGCGGTTTGTACGGCGCATGACCTTCAAACATATCGCAGATTACACCTTCATCCAGCGCGCGGCGGGCTTCGGCAGGAAGCTGTGGGTAAGGCAGATTGTTTTCTGCTTCCAGTGCCAGAAAATGGCGCTTCTGCTCCGGGCTAAGCACTGGGCTGGTGACACTTTGCTGGCAACGTTGTTGCAGTGCATTTTCATCAGAAGGGGGCATCTTCTTTTCCtt                                                                                                                                                                                                                                >  2:300934/1‑251 (MQ=255)
                      gAAAACGGGCGTAATCGGGTAAGACATAGCGCGGTTTGTACGGCGCATGACCTTCAAACATATCGCAGATTACACCTTCATCCAGCGCGCGGCGGGCTTCGGCAGGAAGCTGTGGGTAAGGCAGATTGTTTTCTGCTTCCAGTGCCAGAAAATGGCGCTTCTGCTCCGGGCTAAGCACTGGGCTGGTGACAATTTGCTGGCAACGTTGTTGCAGTGCATTTTCATCAGAAGTGGGCATCTTCTTTTCCttt                                                                                                                                                                                                                               <  1:426035/251‑1 (MQ=255)
                             ggCGTAATCGGGGAAGACATAGCGCGGTTTGTACGGCGCATGACCTTCAAACATATCGCAGATTTCACCTTCATCCAGCGCGCGGCGGGCTTCGGCAGGAAGCTGTGGGTAAGGCAGATTGTTTTCTGCTTCCAGTGCCAGAAAATGGCGCTTCTGCTCCGGGCTAAGCACTGGGCTAGTGACAATTTGCTGGCAACGTTGTTGCAGTGCATTTTCATGAGAAGTGGGCATCTTCTTTTCCTTTTATGCCg                                                                                                                                                                                                                        <  2:100435/251‑1 (MQ=255)
                                  aaTCGGGTAAGACATAGCGCGGTTTGTACGGCGCATGACCTTCAAACATATCGCAGATTACACCTTCATCCAGCGCGCGGCGGGCTTCGGCAGGAAGCTGTGGGTAAGGCAGATTGTTTTCTGCTTCCAGTGCCAGAAAATGGCGCTTCTGCTCCGGGCTAAGCACTGGGCTGGTGACAATTTGCTGGCAACGTTGTTGCAGTGCATTTTCATGAGAAGTGGGCATCTTCTTTTCCTTTTATGCCGAAgg                                                                                                                                                                                                                    <  2:195396/250‑1 (MQ=255)
                                      gggTAAGACATAGCGCGGTTTGTACGGCGCATGACCTTCAAACATATCGCAGATTACACCTTCATCCAGCGCGCGGCGGGCTTCGGCAGGAAGCTGTGGGTAAGGCAGATTGTTTTCTGCTTCCAGTGCCAGAAAATGGCGCTTCTGCTCCGGGCTAAGCACTGGGCTGGTGACAATTTGCTGGCAACGTTGTTGCAGTGCATTTTCATGAGAAGTGGGCATCTTCTTTTCCTTTTATGCCGAAGGTGATg                                                                                                                                                                                                               <  1:131054/251‑1 (MQ=255)
                                        gTAAGACATAGCGCGGTTTGTACGGCGCATGACCTTCAAACATATCGCAGATTACACCTTCATCCAGCGCGCGGCGGGCTTCGGCAGGAAGCTGTGGGTAAGGCAGATTGTTTTCTGCTTCCAGTGCCAGAAAATGGCGCTTCTGCTCCGGGCTAAGCACTGGGCTGGTGACAATTTGCTGGCAACGTTGTTGCAGTGCATTTTCATCAGAAGTGGGCATCTTCTTTTCCTTTTATGCCGAAGGTGATgcg                                                                                                                                                                                                             <  2:272793/251‑1 (MQ=255)
                                          aaGACATAGCGCGGTTTGTACGGCGCATGACCTTCAAACATATCGCAGATTACACCTTCATCCAGCGCGCGGCGGGCTTCGGCAGGAAGCTGTGGGTAAGGCAGATTGTTTTCTGCTTCCAGTGCCAGAAAATGGCGCTTCTGCTCCGGGCTAAGCACTGGGCTGGTGACAATTTGCTGGCAACGTTGTTGCAGTGCATTTTCATCAGAAGTGGGCATCTTCTTTTCCTTTTATGCCGAAGGTGATGCGcc                                                                                                                                                                                                           <  1:433611/251‑1 (MQ=255)
                                                  gcgcGGTTTGTACGGCGCATGACCTTCAAACATATCGCAGATTACACCTTCATCCAGCGCGCGGCGGGCTTCGGCAGGAAGCTGTGGGTAAGGCAGATTGTTTTCTGCTTCCAGTGCCAGAAAATGGCGCTTCTGCTCCGGGCTAAGCACTGGGCTGGTGACAATTTGCTGGCAACGTTGTTGCAGTGCATTTTCATCAGAAGTGGGCATCTTCTTTTCCTTTTATGCCGAAGGTGATGCGCCATTGTaag                                                                                                                                                                                                   <  1:25642/251‑1 (MQ=255)
                                                    gcgGTTTGTACGGCGCATGACCTTCAAACATATCGCAGATTACACCTTCATCCAGCGCGCGGCGGGCTTCGGCAGGAAGCTGTGGGTAAGGCAGATTGTTTTCTGCTTCCAGTGCCAGAAAATGGCGCTTCTGCTCCGGGCTAAGCACTGGGCTGGTGACAATTTGCTGGCAACGTTGTTGCAGTGCATTTTCATGAGAAGTGGGCATCTTCTTTTCCTTTTATGCCGAAGGTGATGCGCCATTGTaagaa                                                                                                                                                                                                 <  1:85859/251‑1 (MQ=255)
                                                         ttGTACGGCGCATGACCTTCAAACATCTCTCAGATTACTCCTTCACCCAGCGCGCGGCGGGCTTCGGCAGGAAGCTGGGGGTAAGGCAGATTGTTTTCGGCTGCCAGTGCCAGAAAATGGCTCTTCTGCTCCGGGCTAAGCACTGGGCTGGTGACAATTTGCTGGCACCGTTGTTGCAGTGCATTTTCATCAGAAGTGGGAATCGTCTTTTCCTTTTATGCCGAATGTGATCCGCCATTGTAAGAAGttt                                                                                                                                                                                             <  2:310672/250‑1 (MQ=255)
                                                         ttGTACGGCGCATGACCTTCAAACATATCGCAGATTACACCTTCATCCAGCGCGCGGCGGGCTTCGGCAGGAAGCTGTGGGTAAGGCAGATTGTTTTCTGCTTCCAGTGCCAGAAAATGGCGCTTCTGCTCCGGTCTAAGCACTGGGCTGGTGACAATTTGCTGGCAACGTTGTTGCAGTGCATTTTCATCCGAAGTGGGCATCTTCTTTTCCTTTTATGCCGAAGGTGGTGCGCCATTGTAAGAAGtttt                                                                                                                                                                                            >  2:11637/1‑250 (MQ=255)
                                                            tACGGCGCATGACCTTCAAACATATCGCAGATTACACCTTCATCCAGCGCGCGGCGGGCTTCGGCAGGAAGCTGTGGGTAAGGCAGATTGTTTTCTGCTTCCAGTGCCAGAAAATGGCGCTTCTGCTCCGGGCTAAGCACTGGGCTGGTGACAATTTGCTGGCAACGTTGTTGCAGTGCATTTTCATCAGAAGTGGGCATCTTCTTTTCCTTTTATGCCGAAGGTGATGCGCCATTGTAAGAAGTTTCGTg                                                                                                                                                                                         >  1:131280/1‑251 (MQ=255)
                                                                    aTGACCTTCAAACATATCGCAGATTACACCTTCATACAGCGCGCGGCGGGCTTCGGCAGGACGCTGTGGGTAAGGCAGATTGTTTTCTGCTTCCAGTGCCAGAAAATGGCGCTTCTGCTCCGGGCTAAGCACTGGGCTGGGGACAATTTGCTGGCAACGTTGTTGCAGTGCATTTTCTTCCGAAGTGGGCATCTTCTTTTCCTTTTATGCCGAAGGTGATGCGCCATTGTAAGATGTTTCGTGATGttaac                                                                                                                                                                                 >  2:174703/1‑248 (MQ=255)
                                                                     tGACCTTCAAACATATCGCAGATTACACCTTCATCCAGCGCGCGGCGGGCTTCGGCAGGAAGCTGTGGGTAAGGCAGATTGTTTTCTGCTTCCAGTGCCAGAAAATGGCGCTTCTGCTCCGGGCTAAGCACTGGGCTGGTGACAATTTGCTGGCAACGTTGTTGCAGTGCATTTTCATCAGAAGTGGGCATCTTCTTTTCCTTTTATGCCGAAGGTGATGCGCCATTGTAAGAAGTTTCGTGATGTTCACt                                                                                                                                                                                >  1:324659/1‑251 (MQ=255)
                                                                              aaCATATCGCAGATTTCAGCGTTATCCAGCGCGCGGCGGGTGTCGGCAGGAAGCTGTGGGTAAGGCAGATTGTTTTCTGCTTCCAGTGCCAGAAAATGGCGCTTCTGCTCCGGGCTAAGCACTGGGCTGGTGACAATTTGCTGGCAACGTTGTTGCAGTGCATTTTCATCAGAAGTGGGCATCTTCTTTTCCTTTTATGCCGAAGGTGATGCGCCATTGTAAGAAGTTTCGTGATGTTCACTTTGATCCTg                                                                                                                                                                       <  2:177662/251‑1 (MQ=255)
                                                                                cATATCGCAGATTACACCTTCATCCAGCGCGCGGCGGGCTTCGGCAGGGAGCTGTGGGTAAGTCAGATTGTTGTCTGCTTCCAGTGCCAGAAAATGGCGCTTCTGCTCCGGGCTAAGAACTGGGCTGGTTACAATTTGCTGGAAACGGTGTTGCAGTGCATTTTCATCGGAAGTGGGCATCTTCTTTTCCTTTTATGCCGAAGATGATGCGCAGTTGTCAGGAGTTTCGTGATGTTCCCTTTGCTCCTGAt                                                                                                                                                                     >  1:39958/1‑251 (MQ=255)
                                                                                 atatCGCAGATTACACCTTCATCCAGCGCGCGGCGGGCTTCGGCAGGAAGCTGTGGGTAAGGCAGATTGTTTTCTGCTTCCAGTGCCAGAAAATGGCGCTTCTGCTCCGGGCTAAGCACTGGGCTGGTGACAATTTGCTGGCAACGTTGTTGCAGTGCATTTTCATCAGAAGTGGGCATCTTCTTTTCCTTTTATGCCGAAGGTGATGCGCCATTGTAAGAAGTTTCGTGATGTTCACTTTGATCCTGATg                                                                                                                                                                    <  2:308255/251‑1 (MQ=255)
                                                                                   atCGCAGATTACACCTTCATCCAGCGCGCGGCGGGCTTCGGCAGGAAGCTGTGGGTAATGCAGATTGTTTTCTGCTTCCAGTGCCAGAAAATGGCGCTTCTGCTACGGGCTAAGCACTGGGCTGGTGACAATTTGCTGGCAACGTTGTTGCAGTGCATTTTCATCAGAAGTGGGCATCTTCTTTTCCTTTTATGCCGAAGGTGGTGCGCCATTGTAAGAAGTTTCGGGATGTTCACTTTGATCCTGATg                                                                                                                                                                    >  2:135592/1‑249 (MQ=255)
                                                                                   atCGCAGATTACACCTTCATCCAGCGCGCGGCGGGCTTCGGCAGGAAGCTGTGGGTAAGGCAGATTGTTTTCTGCTTCCAGTGCCAGAAAATGGCGCTTCTGCTCCGGGCTAAGCACTGGGCTGGTGACAATTTGCTGGCAACGTTGTTGCAGTGCATTTTCATCAGAAGTGGGCATCTTCTTTTCCTTTTATGCCGAAGGTGATGCGCCATTGTAAGAAGTTTCGTGATGTTCACTTTGATCCTGATgag                                                                                                                                                                  <  1:134592/251‑3 (MQ=255)
                                                                                   atCGCAGATTACACCTTCATCCAGCGCGCGGCGGGCTTCGGCAGGAAGCTGCGGGTAAGGCACATTGTTAACTGATTCCAGTGCCAGAAAATGGCGCTTCTGCCCCGGGATAAGCACTGGGCTGGTGCCACTTTGCTGGCAACGTTGTTGCAGTGCATTTTAATAAGCAGAGGGAATCTTCTTTTCCTTTTATGCCGAAGGTGATGCGCCATTGTAAGAAGTTTCGTGATGTTCACTTTGATACTGATg                                                                                                                                                                    >  1:74908/1‑249 (MQ=255)
                                                                                               cccTTCATCCATCGTGGGGCGGGCTTCGGCAGGAAGCTGTGGGTAAGGCAGATTGTTGTCTGCTTCCAGTGCCAGAAAATGGCGCTTCTGCTCCGGGCTAAGCACTGGGCTGGTGACAATTTGCTGGCAACGTTGTTGCAGTGCATTTTCATCAGAAGTGGGCATCTTCTTTTCCTTTTATGCCGAAGGTGATGCGCCATTGTAAGAAGTTTCGTGATGTTCACTTTGATCCTGATGAGTTTGCCACCACt                                                                                                                                                      <  2:59677/250‑1 (MQ=255)
                                                                                                   tCATCCAGCGCTCGGCGGGCTTCGGCAGGAAGCTGTGGGTAAGGCAGATTGTTTTCTGCTTCCAGTGCCAGAAAATGGCGCTTCTGCTCCGGGCTAAGCACTGGGCTGGTGACAATTTGCTGGCAACGTTGTTGCAGTGCATTTTCATCAGAAGTGGGCATCTTCTTTTCCTTTTATGCCGAAGGTGATGCGCCATTGTAAGAAGTTTCGTGATGTTCACTTTGATCCTGATGAGTTTGCCACCACTGAc                                                                                                                                                   <  2:490340/250‑1 (MQ=255)
                                                                                                       ccAGCTCGCGGCTGGCTTCGGCAGGAAGCTGTGGGTAAGGCAGATTGTTTTCTGCTTCCAGTGCCAGAAAATGGCGCTTCTGCTCCGGGCTAAGCACTGGGCTGGTGACAATTTGCTGGCAACGTTGTTGCAGTGCATTGTCATCAGAAGTGGGCATCTTCTTTTCCTTTTATGCCGAAGGTGATGCGCCATTGTAAGAAGTTTCGTGATGTTCACTTTGATCCTGATGAGTTTGCCACCACTGACGcatt                                                                                                                                              <  1:42647/251‑1 (MQ=255)
                                                                                                       ccAGCGCGCGGCGGGCTTCGGCAGGAAGCTGTGGGGAATGCAGATTGGTTTCTGCTTCCAGTGCCAGAAAATGGCGCTTCTGCTCCGGGCCAAGCACTGGGCTGGTGACAATTTGCTGGCAACGTTGTTGCAGTGCATTTGCATCAGAAGTGGGCATCTTCTTTACCTTTTATGCCGAAGGTGATGCTCCATTGTAAGAAGTTTTGTGATGTTCGCGTTGATCCTGTTGAGGTTGCAACCACTGACGcatt                                                                                                                                              <  2:310449/251‑1 (MQ=255)
                                                                                                                   gggCTTCGGCAGGAAGCTGTGGGTAAGGCAGATTGTTTTCTGCTTCCAGTGCCAGAAAATGGCGCTTCTGCTCCGGGCTAAGCACTGGGCTGGTGACAATTTGCTGGCAACGTTGTTGCAGTGCATTTTCATCAGAAGTGGGCATCTTCTTTTCCTTTTATGCCGAAGGTGATGCGCCATTGTAAGAAGTTTCGTGATGTTCACTTTGATCCTGATGAGTTTGCCACCACTGACGCATTCATTTGAAAGTg                                                                                                                                  >  1:284023/1‑251 (MQ=255)
                                                                                                                        gctcttccgatcTGTGGGTAAGGCAGATTGTTTTCTGCTTCCAGTGCCAGAAAATGGCGCTTCTGCTCCGGGCTAAGCACTGGGCTGGTGACAATTTGCTGGCAACGTTGTTGCAGTGCATTTTCATCAGAAGTGGGCATCTTCTTTTCCTTTTATGCCGAAGGTGATGCGCCATTGTAAGAAGTTTCGTGATGTTCACTTTGATCCTGATGAGTTTGCCACCACTGACGCATTCATTTGAAAGTGAatt                                                                                                                              >  2:369286/12‑250 (MQ=255)
                                                                                                                            cAGGAAGCTGTGGGTAAGGCAGATTGTTTTCTGCTTCCAGTGCCAGAAAATGGCGCTTCTGCTCCGGGCTAAGCACTGGGCTGGTGACAATTTGCTGGCAACGTTGTTGCAGTGCATTTTCATCAGAAGTGGGCATCTTCTTTTCCTTTTATGCCGAAGGTGATGCGCCATTGTAAGAAGTTTCGTGATGTTCACTTTGATCCTGATGAGTTTGCCACCACt                                                                                                                                                      >  1:403388/1‑222 (MQ=255)
                                                                                                                            cAGGAAGCTGTGGGTAAGGCAGATTGTTTTCTGCTTCCAGTGCCAGAAAATGGCGCTTCTGCTCCGGGCTAAGCACTGGGCTGGTGACAATTTGCTGGCAACGTTGTTGCAGTGCATTTTCATCAGAAGTGGGCATCTTCTTTTCCTTTTATGCCGAAGGTGATGCGCCATTGTAAGAAGTTTCGTGATGTTCACTTTGATCCTGATGAGTTTGCCACCACt                                                                                                                                                      <  2:403388/222‑1 (MQ=255)
                                                                                                                            cAGGAAGCTGTGGGTAAGGCAGATTGTTTTCTGCTTCCAGTGCCAGAAAATGGCGCTTCTGCTCCGGGCTAAGCACTGGGCTGGTGACAATTTGCTGGCAACGTTGTTGCAGTGCATTTTCATCAGAAGTGGGCATCTTCTTTTCCTTTTATGCCGAAGGTGATGCGCCATTGTAAGAAGTTTCGTGATGTTCACTTTGATCCTGATGAGTTTGCCACCACTGACGCATTCATTTGAAAGTGAATTATTTg                                                                                                                         <  1:364806/251‑1 (MQ=255)
                                                                                                                                 aGCTGTGGGTAAGGCAGGTTGTTTTCTGCTTCCAGTGCCAGAAAATGGCGCTTCTGCTCCGGGCTAAGCACTGGGCTGGTGACAATTTGCTGGCAACGTTGTTGCAGTGCATTTTCATCAGAAGTGGGCATCTTCTTTTCCTTTTATGCCGAAGGTGATGCGCCATTGTAAGAAGTTTCGTGATGTTCACTTTGATCCTGATGAGTTTGCCACCACTGACGCATTCATTTGAAAGTGAATCATGTGAACCa                                                                                                                    <  2:4027/251‑1 (MQ=255)
                                                                                                                                     gtgGGTAAGGCAGATTGTTTTCTGCTTCCAGTGCCAGAAAATGGCGCTTCTGCTCCGGGCTAAGCACTGGGCTGGTGACAATTTGCTGGCAACGTTGTTGCAGTGCATTTTCATCAGAAGTGGGCATCTTCTTTTCCTTTTATGCCGAAGGTGATGCGCCATTGTAAGAAGTTTCGTGATGTTCACTTTGATCCTGATGAGTTTGCCACCACTGACGCATTCATTTGAAAGTGAATTATTTGATCCAGAt                                                                                                                 <  1:422428/250‑1 (MQ=255)
                                                                                                                                            aGGCAGATTGGGGTCTGCTTCCAGTGCCAGAAAAGGGCGCTTTTGCTTCGGGCGAAACATGGGGCTGGTGACAATTTGCTGGCAACGTTGTAGCAGTGCATTTTCATCAGAAGGGGGCATCTTCTTTTCCTTTTATGCCGAAGGTGATGCGTCATTGTAAGAAGTTTCGTGATGTTCACTTTGATCCTGATGAGTTTACCACCACTGACGCATTCAGTTGAAATTGAATGATTTGAACCCGATCACATTAc                                                                                                         <  2:333485/251‑1 (MQ=255)
                                                                                                                                              gCAGATTGTTTTCTGCTTCCAGTGCCAGAAAATGGCGCTTCTGCTCCGGGCTAAGCACTGGGCTGGTGACAATTTGCTGGCAACGTTGTTGCAGTGCATTTTCATCAGAAGTGGGCATCTTCTTTTCCTTTTATGCCGAAGGTGATGCGCCATTGTAAGAAGTTTCGTGATGTTCACTTTGATCCTGATGAGTTTGCCACCACTGACGCATTCATTTGAAAGTGAATTATTTGAACCAGATCGCATTAc                                                                                                         >  1:385390/1‑249 (MQ=255)
                                                                                                                                                aGATTGTTTTCTGCTTCCAGTGCCAGAAAATGGCGCTTCTGCTCCGGGCTAAGCACTGGGCTGGTGACAATTTGCTGGCAACGTTGTTGCAGTGCATTTTCATCAGAAGTGGGCATCTTCTTTTCCTTTTATGCCGAAGGTGATGCGCCATTGTAAGAAGTTTCGTGATGTTCACTTTGATCCTGATGAGTTTGCCACCACTGACGCATTCATTTGAAAGTGAATTATTTGAACCAGATCGCATTACAGTg                                                                                                     >  1:237734/1‑251 (MQ=255)
                                                                                                                                                 gATTGTTTTCTGCTTCCAGTGCCAGAAAATGGCGCTTCTGCTCCGGGCTAAGCACTGGGCTGGTGACAATTTGCTGGCAACGTTGTTGCAGTGCATTTTCATCAGAAGTGGGCATCTTCTTTTCCTTTTATGCCGAAGGTGATGCGCCATTGTAAGAAGTTTCGTGATGTTCACTTTGATCCTGATGAGTTTGCCACCACTGACGCATTCATTTGAAAGTGAATTATTTGAACCAGATCGCATTACAGTg                                                                                                     >  1:344974/1‑250 (MQ=255)
                                                                                                                                                    tGTTTTCTGCTTCCAGTGCCAGAAAATGGCGCTTCTGCTCCGGGCTAAGCACTGGGCTGGTGACAATTTGCTGGCAACGTTGTTGCAGTGCATTTTCATCAGAAGTGGGCATCTTCTTTTCCTTTTATGCCGAAGGTGATGCGCCATTGTAAGAAGTTTCGTGATGTTCACTTTGATCCTGATGAGTTTGcca                                                                                                                                                           <  1:386875/193‑1 (MQ=255)
                                                                                                                                                    tGTTTTCTGCTTCCAGTGCCAGAAAATGGCGCTTCTGCTCCGGGCTAAGCACTGGGCTGGTGACAATTTGCTGGCAACGTTGTTGCAGTGCATTTTCATCAGAAGTGGGCATCTTCTTTTCCTTTTATGCCGAAGGTGATGCGCCATTGTAAGAAGTTTCGTGATGTTCACTTTGATCCTGATGAGTTTGcca                                                                                                                                                           >  2:386875/1‑193 (MQ=255)
                                                                                                                                                     gTTTTCTGCTTCCAGTGCCAGAAAATGGCGCTTCTGCTCCGGGCTAAGCACTGGGCTGGTGACAATTTGCTGGCAACGTTGTTGCAGTGCATTTTCATCAGAAGTGGGCATCTTCTTTTCCTTTTATGCCGAAGGTGATGCGCCATTGTAAGAAGTTTCGTGATGTTCACTTTGATCCTGATGAGTTTGCCACCACTGACGCATTCATTTGAAAGTGAATTATTTGAACCAGATCGCATTACAGTGATGCa                                                                                                <  1:371921/251‑1 (MQ=255)
                                                                                                                                                       tttCTGCTTCCAGTGCCAGAAAATGGCGCTTCTGCTCCGGGCTAAGCACTGGGCTGGTGACAATTTGCTGGCAACGTTGTTGCAGTGCATTTTCATCAGAAGTGGGCATCTTCTTTTCCTTTTATGCCGAAGGTGATGCGCCATTGTAAGAAGTTTCGTGATGTTCACTTTGATCCTGATGAGTTTGCCACCACTGACGCATTCATTTGAAAGTGAATTATTTGAACCAGATCGCATTACAGTGATGCaa                                                                                               >  2:446261/1‑250 (MQ=255)
                                                                                                                                                            gCTTCCAGTGCCAGAAAATGGCGCTTCTGCTCCGGGCTAAGCACTGGGCTGGTGACAATTTGCTGGCAACGTTGTTGCAGTGCATTTTCATCAGAAGTGGGCATCTTCTTTTCCTTTTATGCCGAAGGTGATGCGCCATTGTAAGAAGTTTCGTGATGTTCACTTTGATCCTGATGAGTTTGCCACCACTGACGCATTCATTTGAAAGTGAATTATTTGAACCAGATCGCATTACAGTGATGCAAACtt                                                                                           <  1:449957/249‑1 (MQ=255)
                                                                                                                                                            gCTTCCAGTGCCAGAAAATGGCGCTTCTGCTCCGGGCTAAGCACTGGGCTGGTGACAATTTGCTGGCAACGTTGTTGCAGTGCATTTTCATCAGAAGTGGGCATCTTCTTTTCCTTTTATGCCGAAGGTGATGCGCCATTGTAAGAAGTTTCGTGATGTTCACTTTGATCCTGATGAGTTTGCCACCACTGACGCATTCATTTGAAAGTGAATTATTTGAACCAGATCGCATTACAGTGATGCAAACt                                                                                            <  1:354263/248‑1 (MQ=255)
                                                                                                                                                                 cAGTGCCAGAAAATGGCGCTTCTGCGCCGGGCTAAGTACTGGGCTGGTGACAATTTGCTTGCAACGTTGTTGCAGTGCATTTTCATCAGAAGTGGGCATCTTCTTTTCCTTTTATGCCGAAGGTGATGCGCCATTGTAAGAAGTTTCGTGATGTTCACTTTGATCCTGATGAGTTTGCCACCACTGACGCATTCATTTGAAAGTGAATTATTTGAACCAGATCGCATTACAGTGATGCAAACTTGTAAGTa                                                                                    <  2:131280/251‑1 (MQ=255)
                                                                                                                                                                   gTGCCAGAAAATGGCGCTTCTGCTCCGGGCTAAGCACTGGGCTGGTGACAATTTGCTGGCAACGTTGTTGCAGTGCATTTTCATCAGAAGTGGGCATCTTCTTTTCCTTTTATGCCGAAGGTGATGCGCCATTGTAAGAAGTTTCGTGATGTTCACTTTGATCCTGATGAGTTTGCCACCACTGACGCATTCATTTGAAAGTGAATTATTTGAACCAGATCGCATTACAGTGATGCAAACTTGTAAGt                                                                                     >  2:239559/1‑248 (MQ=255)
                                                                                                                                                                   gTGCCAGAAAATGGCGCTTCTGCTCCGGGCTAAGCACTGGGCTGGTGACAATTTGCTGGCAACGTTGTTGCAGTGCATTTTCATCAGAAGTGGGCATCTTCTTTTCCTTTTATGCCGAAGGTGATGCGCCATTGTAAGAAGTTTCGTGATGTTCACTTTGATCCTGATGAGTTTGCCACCACTGACGCATTCATTTGAAAGTGAATTATTTGAACCAGATCGCATTACAGTGATGCAAACTTGTAAGTAGa                                                                                  <  1:369286/251‑1 (MQ=255)
                                                                                                                                                                     gCCAGAAAATGGCGCTTCTGCTCCGGGCTAAGCACTGGGCTGGTGACAATTTGCTGGCAACGTTGTTGCAGTGCATTTTCATCAGAAGTGGGCATCTTCTTTTCCTTTTATGCCGAAGGTGATGCGCCATTGTAAGAAGTTTCGTGATGTTCACTTTGATCCTGATGAGTTTGCCACCACTGACGCATTCATTTGAAAGTGAATTATTTGAACCAGATCGCATTACAGTGATGCAAACTTGTAAGTAGAtt                                                                                >  1:173630/1‑251 (MQ=255)
                                                                                                                                                                           aaaTGGCGCTTCTGCTCCGGGCTAAGCACTGGGCTGGTGACAATTTGCTGGCAACGTTGTTGCAGTGCATTTTCATCAGAAGTGGGCATCTTCTTTTCCTTTTATGCCGAAGGTGATGCGCCATTGTAAGAAGTTTCGTGATGTTCACTTTGATCCTGATGAGTTTGCCACCACTGACGCATTCATTTGAAAGTGAATTATTTGAACCAGATCGCATTACAGTGATGCAAACTTGTAAGTAGATTTCCTTa                                                                          <  2:173630/251‑1 (MQ=255)
                                                                                                                                                                            aaTGGCGCTTCTGCACCGGGCTAATCACTGGGCTGGTTACAATTTGCGGGCAACGTTGTTGCAGTGCATTTTCATCAGAAGGGGGCATCTTCTTTTCCTTTTATGCCGAAGTTGATGCGCCATTGTAAGAAGTTTCGTGATGTTCACTTTGATCCTGATGAGTTTGCCACCACTGACGCATTCATTTGAAAGTGAATTATTTGAACCAGATCGCATTACAGTGATTCAAACGTGTAAGTAGATTTCCTTaa                                                                         <  2:32178/251‑1 (MQ=255)
                                                                                                                                                                               ggCGCTTCTGCTCCGGGCTAAGCACTGGGCTGGTGACAATTTGCTGGCAACGTTGTTGCAGTGCATTTTCATCAGAAGTGGGCATCTTCTTTTCCTTTTATTCCGAAGGTGATGCGCCATTGTAAGAAGTTTCGTGATGTTCAGTTTGATCCTGATGAGTTTGCCACCACTGTCGCATTCATTTGAAAGTGAATTATGTGAACCAGATCGCATTACAGTGATGCAAACTTGTAAGTAGATTTCCTTAATtg                                                                      <  1:464320/251‑1 (MQ=255)
                                                                                                                                                                               ggCGCTTCTGCTCCGGGCTAAGCACTGGGCTGGTGACAATTTGCTGGCAACGTTGTTGCAGTGCATTTTCATCAGAAGTGGGCATCTTCTTTTCCTTTTATGCCGAAGGTTATGCGCCATTGTAAGAAGTTTCGTGATGTTCACTTTGATCCTGATGAGGTTGCCACCACTGACGCAGTCATTTGAAAGTGAATTATTTGAACCAGATCGCATTCCAGTGATGCAAACTTGTAAGTAGATTTCCTTAATtg                                                                      <  1:221948/251‑1 (MQ=255)
                                                                                                                                                                                     tCTGCTCCGGGCTAAGCACTGGGCTGGTGACAATTTGCTGGCAACGTTGTTGCAGTGCATTTTCATCAGAAGTGGGCATCTTCTTTTCCTTTTATGCCGAAGGTGATGCGCCATTGTAAGAAGTTTCGTGATGTTCACTTTGATCCTGATGAGTTTGCCACCACTGACGCATTCATTTGAAAGTGAATTATTTGAACCAGATCGCATTACAGTGATGCAAACTTGTAAGTAGATTTCCTTAATTGTGAtg                                                                 <  2:203702/250‑1 (MQ=255)
                                                                                                                                                                                     tCTGCTCCGGGCTAAGCACTGGGCTGGTGACAATTTGCTGGCAACGTTGTTGCAGTGCATTTTCATCAGAAGTGGGCATCTTCTTTTCCTTTTATGCCGAAGGTGATGCGCCATTGTAAGAAGTTTCGTGATGTTCACTTTGATCCTGATGAGTTTGCCACCACTGACGCATTCATTTGAAAGTGAATTATTTGAACCAGATCGCATTACAGTGATGCAAACTTGTAAGTAGATTTCCTTAATTGTGAtg                                                                 <  2:324458/250‑1 (MQ=255)
                                                                                                                                                                                                  aaGCACTGGGCTGGTGACAATTTGCTGGCAACGTTGTTGCAGTGCATTTTCATCAGAAGTGGGCATCTTCTTTTCCTTTTATGCCGAAGGTGATGCGCCATTGTAAGAAGTTTCGTGATGTTCACTTTGATCCTGATGAGTTTGCCACCACTGACGCATTCATTTGAAAGTGAATTATTTGAACCAGATCGCATTACAGTGATGCAAACTTGTAAGTAGATTTCCTTAATTGTGATGTATATCGAAgtgt                                                    <  1:249630/250‑1 (MQ=255)
                                                                                                                                                                                                  aaGCACTGGGCTGGTGACAATTTGCTGGCAACGTTGTTGCAGTGCATTTTCATCAGAAGTGGGCATCTTCTTTTCCTTTTATGCCGAAGGTGATGCGCCATTGTAAGAAGTTTCGTGATGTTCACTTTGATCCTGATGAGTTTGCCACCACTGACGCATTCATTTGAAAGTGAATTATTTGAACCAGATCGCATTACAGTGATGCAAACTTGTAAGTAGATTTCCTTAATTGTGATGTATATCGAAgtgt                                                    <  1:290702/250‑1 (MQ=255)
                                                                                                                                                                                                    gCACTGGGCTGGTGACAATTTGCTGGCAACGTTGTTGCAGTGCATTTTCATCAGAAGTGGGCATCTTCTTTTCCTTTTATGCCGAAGGTGATGCGCCATTGTAAGAAGTTTCGTGATGTTCACTTTGATCCTGATGAGTTTGCCACCACTGACGCATTCATTTGAAAGTGAATTATTTGAACCAGATCGCATTACAGTGATGCAAACTTGTAAGTAGATTTCCTTAATTGTGATGTATATCGAAGTGTGtt                                                 >  1:153668/1‑251 (MQ=255)
                                                                                                                                                                                                                                               aTTTTCATCAGAAGTGGGAATCTTCTTTTCCTTTTATGCCGAAGGTGATGCGACATTGTAAGAAGTTTCGTGATGTTCACTTTGATCCTGATGAGTTTGCCACCACTGACGCATTCATTTGAAAGTGAATTATTTGAACCAGATCGCATTACAGTGATGCAAACTTGTAAGTAGATTTCCTTAATTGTGATGTATATCGAAGTGTGTTGCGGAGTAGATGTTAGAATATTAACAAACTAGAAAGGGGAAtt      >  1:169379/1‑251 (MQ=255)
                                                                                                                                                                                                                                                 tttCATCAGAAGTGGGCATCTTCTTTTCCTTTTATGCCGAAGGTGATGCGCCATTGTAAGAAGTTTCGTGATGTTCACTTTGATCCTGATGAGTTTGCCACCACTGACGAATTCATTTGAAAGTGAATTATTTGAACCAGATCGCATTACAGTGATGCAAACTTGTAAGTAGATTTCCTTAATTGTGATGTATATCGAAGTGTGTTGCGGAGTAGATGTTAGAATACTAACAAACTCGCAAGGTGAAttt     <  1:52836/250‑1 (MQ=255)
                                                                                                                                                                                                                                                   tcatcaGAAGTGGGCATCTTCTTTTCCTTTTATGCCGAAGGTGATGCGCCATTGTAAGAAGTTTCGTGATGTTCAATTTGATCCTGATGAGTTTGCCACCACTGACGCATTCATTTGAAAGTGAATTATTTGAACCAGATCGCATTACAGTGATGCAAACTTGTAAGTAGATTTCCTTAATTGTGATGTATATCGAAGTGTGTTGCGGAGTAGATGTTAGAGTACTAACAAACTCGCAAGGTGAATTTTAt  >  1:196023/1‑251 (MQ=255)
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                      
AGCCATTCGGAACCGTTCGCCAGAAAACGGGCGTAATCGGGTAAGACATAGCGCGGTTTGTACGGCGCATGACCTTCAAACATATCGCAGATTACACCTTCATCCAGCGCGCGGCGGGCTTCGGCAGGAAGCTGTGGGTAAGGCAGATTGTTTTCTGCTTCCAGTGCCAGAAAATGGCGCTTCTGCTCCGGGCTAAGCACTGGGCTGGTGACAATTTGCTGGCAACGTTGTTGCAGTGCATTTTCATGAGAAGTGGGCATCTTCTTTTCCTTTTATGCCGAAGGTGATGCGCCATTGTAAGAAGTTTCGTGATGTTCACTTTGATCCTGATGCGTTTGCCACCACTGACGCATTCATTTGAAAGTGAATTATTTGAACCAGATCGCATTACAGTGATGCAAACTTGTAAGTAGATTTCCTTAATTGTGATGTGTATCGAAGTGTGTTGCGGAGTAGATGTTAGAATACTAACAAACTCGCAAGGTGAATTTTAT  >  NC_000913/4616806‑4617299

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑