| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,616,682 | C→T | P128P (CCG→CCA) | yjjI ← | DUF3029 domain‑containing protein YjjI |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,616,682 | 0 | C | T | 92.2% | 199.0 / 5.3 | 77 | P128P (CCG→CCA) | yjjI | DUF3029 domain‑containing protein YjjI |
| Reads supporting (aligned to +/- strand): ref base C (4/2); new base T (39/32); total (43/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.89e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.70e-01 | |||||||||||
ATTCACAGATGTTCTTCGCCACTTCCAGCAGCAGGTCATCAGGGGTGATTTCAGGATCGTAGATAAAGGTCAGGTTCGGTGAAACCTGCTTCAACTCTGCATCTGCACGTAAGATCGCGCGGGTAATGGGCGAATCAGAC‑GGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAA‑CATCGATCTCGTCTTGTGTTAGAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAG‑AGAGTGCGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGAAAACGGGCGTAATCGGGTAAGACATAGCGCGGTTTGTACGGCGCATGACCTTCAAACATATCGCAGATTACACCTTCATCCAGCGCGCGGCGGGCTTC > NC_000913/4616435‑4616927 | aTTCACAGATGTTCTTCGCCACTTCCAGCAGCAGGTCATCAGGGGTGATTTCAGGATCGTAGATAAAGGTCAGGTTCGGTGAAACCTGCTTCAACTCTGCATCTGCACGTAAGATCGCGCGGGTAATGGGCGAATCGGAC‑GGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAA‑CATCGATCTCGTCTTGTGTTAGAATTCTAACATACGGt < 1:17273/251‑1 (MQ=255) cacaGAGGTTCTCCGCCACTTCCAGCAGCAGGTCATCAGGGGTGATTTCAGGATCGTAGATAAAGGTCAGGTTCGGGGAAACCTGCTTCAACTCGGCGTCGGCACGTAAGATCGCGCGGGTAATGGGCGAATCAGAC‑GGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAA‑TATCGATCTCGTCTTGTGTTAGAATTCTAACATATGGTTg < 1:501135/250‑1 (MQ=255) ccACTTCCGGCAGCAGGTCATCAGGGGTGATTTCAGGATCGTCGATCATGGTCAGGTTCGGGGAACCCTGCTTCAACTCTGCGTCTGCACGTAAGATCGCGCGGGTAATGGGCGAATCGGAC‑GGGCCGATATTGGCGTGCATAAAGGAGTCTGGCAGGGTTCTGTCGAGGTGACGCCAGAAACGCTTTATTCGAA‑TATCGATCTAGTCTTGTGTTAGAACTCTAACTTCTAGTTGCAACAACGCAACCaat > 2:221948/1‑249 (MQ=255) aCTTCCAGCAGCAGGTCATCAGGGGTGATTTCAGGATCGTAGATAAAGGTCAGGTTCGGTGAAACCTGCTTCAACTCTGCATCTGCACGTAAGATCGCGCGGGTAATGGGCGAATCAGAC‑GGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAA‑CATCGATCTCGTCTTGTGTTAGAATTCTAACATACGGTTGCAACAACGCATCCAGTTg > 1:100435/1‑251 (MQ=255) aCTTCCAGCAGCAGGTCATCAGGGGTGATTTCAGGATCGTAGATAAAGGTCAGGTTCGGGGAAACCTGCTTCAACTCTGCGTCTGCACGTAAGATCGCGCGGGTAATGGGCGAATCAGAC‑GGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAA‑TATCGATCTCGTCTTGTGTTAGAATTCTAACATATGGTTGCAACAACGCATCCAGTTg < 1:258676/251‑1 (MQ=255) cTTCCAGCAGCAGGTCATCAGGGGTGATTTCAGGATCGTAGATAAAGGTCAGGTTCGGGGAAACCTGCTTCAACTCTGCGTCTGCACGTAAGATCGCGCGGGTAATGGGCGAATCAGAC‑GGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAA‑TATCGATCTCGTCTTGTGTTAGAATTCTAACATATGGTTGCAACAACGCATCCAGTTGc > 1:27454/1‑251 (MQ=255) ttCCAGCAGCAGGTCATCAGGGGTGATTTCAGGATCGTAGATAAAGGTCAGGTTCGGGGAAACCTGCTTCAACTCTGCGTCTGCACGTAAGATCGCGCGGGTAATGGGCGAATCAGAC‑GGGCCGATATTGGCGTGCATAACGGCGTCTGGCAGGGTTCTGTCGCGGAAACGCCAGAAACGTTTTCTTCGAA‑TATCGATCTCGTCTTGAGTTAGAATTATAACATATGGTTGCAACAACGCATCCAGTTGcc > 1:310672/1‑251 (MQ=255) gcagcagGTCATCAGGGGTGATTTCAGGATCGTAGATAAAGGTCAGGTTCGGGGAAACCTGCTTCAACTCTGCGTCTGCACGTAAGATCGCGCGGGTAATGGGCGAATCAGAC‑GGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAA‑TATCGATCTCGTCTTGTGTTAGAATTCTAACATATGGTTGCAACAACGCATCCAGTTGCCCCAgg < 1:124289/251‑1 (MQ=255) agttcatcaGGGGTGATTTCAGGATCGTAGATAAAGGTCAGGTTCGGGGAAACCTGCTTCAACTCTGCGTCTGCACGTAAGATCGCGCGGGTAATGGGCGAATCAGAC‑GGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAA‑TATCGATCTCGTCTTGTGTTAGAATTCTAACATATGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGAc < 1:66501/248‑1 (MQ=255) aggacatcaGGGGTGATTTCAGGATCGTAGATAAAGGTCAGGTTCGGGGAAACCTGCTTCAACTCTGCGTCTGCACGTAAGATCGCGCGGGTAATGGGCGAATCAGAC‑GGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAA‑TATCGATCTCGTCTTGTGTTAGAATTCTAACATATGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGAc < 1:344493/247‑1 (MQ=255) agGTCATCAGGGGTGATTTCAGGATCGTAGATAAAGGTCAGGTTCGGGGAAACCTGCTTCAACTCTGCGTCTGCACGTAAGATCGCGCGGGTAATGGGCGAATCAGAC‑GGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAA‑TATCGATCTCGTCTTGTGTTAGAATTCTAACATATGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGAc > 1:430984/1‑251 (MQ=255) ttCAGGATCGTAGATAAAGGTCAGGTTCGGGGAAACCTGCTTCAACTCTGCGTCTGCACGTAAGATCGCGCGGGTAATGGGCGAATCAGAC‑GGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAA‑TATCGATCTCGTCTTGTGTTAGAATTCTAACATATGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCg > 1:230619/1‑251 (MQ=255) tCAGGATCGTAGATAAAGGTCAGGTTCGGGGAAACCTGCTTCAACTCTGCGTCTGCACGTAAGATCGCGCGGGTAATGGGCGAATCAGAC‑GGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAA‑TATCGATCTCGTCTTGTGTTAGAATTCTAACATATGGTTGCAACAACGCCTCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCg > 2:309527/1‑250 (MQ=255) aGGATCGTAGATAAAGGTCAGGTTCGGGGAAACCTGCTTCAACTCTGCGTCTGCACGTAAGATCGCGCGGGTAATGGGCGAATCAGAC‑GGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGGCGAGGTAACGCCAGAAACGTTTTATTCGAA‑TATCGATCTCGTCTTGTGTTAGAATTCTAACATATGGTTGCAAAAACGCATACAGTTGCCCCAGGTAACCCGGCATCGTTGTGACCgccg > 2:397570/1‑248 (MQ=255) ggATCGTAGATAAAGGTCAGGTTCGGTGAAACCTGCTTCAACTCTGCATCTGCACGTAAGATCGCGCGGGTAATGGGCGAATCAGCC‑GGGCCGATCTTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACTGCAGAAACGTTTTATTCGAA‑CAGCGATCTCGTCTTGGGTTAGAATTCTAACATACTGTTGCAAAAACGCGTACAGTTGCCCCAGGGAGACCGGCATCGATATGACCGACgg > 2:131054/1‑251 (MQ=255) aaaGGTCAGGTTCGGGGAAACCTGCTTCTACTCTGCGTCTGCACGTAAGATCGCGCGGGGAATGGGCGAATCAGAC‑GGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAA‑TATCGATCTCGTCTTGTGTTAGAATTCTAACATTTGGTTGCGACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGGCCGACGGTACGtggtggg > 2:405577/1‑250 (MQ=255) aaaGGTCAGGTTCGGGGAAACCTGCTTCACCTCTGCGTCTGCACGTAAGATCGCGCGGGGAATGGGCGAATCAGAC‑GGGCCGTTATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCTAGGTAACGCCAGAAACGTTTTATTCGAATCA‑CGATCTCGTCTTGTGTTAGAATTCTAACATTTGGTTGCTACTACGCATCCAGTTGCCCGAGGTAGACCGGAATAGGTGTGACCGCCGGTACGTgggggt > 2:405570/1‑251 (MQ=255) ggTCAGGTTCGGGGAAACCTGCTTCAACTCTGCGTCTGCACGTAAGATCGCGCGGGTAATGGGCGAATCAGAC‑GGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAA‑TATCGATCTCGTCTTGTGTTAGAATTCTAACATATGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTaaa < 1:346813/251‑1 (MQ=255) ggTCAGGTTCGAGGAAACCTACTACATCTCTGTGTTTGCACGTAAGACCGCGAGGGTAATGGGCGAACAAGAC‑GGGCCGATATTTGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCATAAACGTTTTATTCGAA‑TATCGATCTCGTCTTGTGTTAGAATTCTAACATATGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTaaa < 2:13469/251‑1 (MQ=255) cggggAAACCTGCTTCAACTCTGAGTCGGCACGTAAGACCGCGCGGTTAATGGGCGAATCAGAC‑GGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAA‑TATCGATCGCGTCTTGTGTTAGAATTCTAACATATGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCTTCGATGTAACCGACGGTACGAGGTGGTAAAGAATGGTca < 1:419849/247‑1 (MQ=255) ggggAAACCTGCTTCAACTCTGCGTCTGCACGTAAGATCGCGCGGGTAATGGGCGACTCAGAC‑GGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAA‑TATCGATCTCGTCTTGTGTTAGAATTCTAACATATGGTTTCAACAACGCATCCCGTTGCCCCAGGGAGACCGGCATCGTTGTGACCGAAGGAACGTGGTGGTGAAGAATGGTc > 2:354263/4‑249 (MQ=255) ggggAAACCTGCTTCAACTCTGCGTCTGCACGTAAGATCGCGCGGGTAATGGGCGAATCAGAC‑GGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAA‑TATCGATCTCGTCTTGTGTTAGAATTCTAACATATGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGGACGTGGTGGTAAAGAATGGTc > 2:445521/4‑249 (MQ=255) gAAACCTGCTTCAACTCTGCGTCTGCACGTAAGATCGCGCGGGTAATGGGCGAATCAGAC‑GGGCCGCTATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAA‑TATCGATCTCGTCTTGTGTTAGAATTCTAACATATGGTTGCAACAACGCATCCAGTTGCCCAAGGTAGACCGGCATCGATGTGACCGACGGTACGGGGGGGTAAAGAATGGtagat > 2:375836/1‑245 (MQ=255) gAAACCTGCTTCAACTCTGCGTCTGCACGTAAGATCGCGCGGGTAATGGGCGAATCAGAC‑GGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAA‑TATCGATCTCGTCTTGTGTTAGAATTCTAACATATGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGt < 1:375836/245‑1 (MQ=255) aaCCTGCTGCAACTCTGCGCCGGCACGTAAGATGTCGCGGGAAATTGGCGATCCGGAC‑GGGCCGATATTGGCGTGCATAAAGGCGGCTGGCAGTGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAA‑TAGCGATCTCGTCTTGTGTTAGAATTCTAACATATGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCAGCGATTTGACCTACGGTACGTGGTGGTAAAGAATGGTCAGCagag < 2:499706/251‑1 (MQ=255) ctgcgtctgcaCGTAAGATCGCGCGGGTAATGGGCGAATCAGAC‑GGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAA‑TATCGATCTCGTCTTGTGTTAGAATTCTAACATATGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAG‑AGAGTGCGtcatc > 1:382502/1‑250 (MQ=255) cgtctgcaCGTAAGATCGCGCGGGTAATGGGCGAATCAGAC‑GGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAA‑TATCGATCTCGTCTTGTGTTAGAATTCTAACATATGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAG‑AGAGTGCGtcatca > 1:210600/3‑248 (MQ=255) gtctgcaCGTAAGATCGCGCGGGTAATGGGCGAATCAGAC‑GGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAA‑TATCGATCTCGTCTTGTGTTAGAATTCTAACATATGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAG‑AGAGTGCGTCATCAAGAt < 1:445521/250‑1 (MQ=255) ggcgGGTAATGGGGGAAT‑AGACCGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAA‑TATCGATCTCGTCTTGTGTTAGAATTCTAACATATGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAG‑AGAGTGCGTCATCAAGATCTTTCGCGCCTtccagc < 2:155541/250‑1 (MQ=255) ggggaaTGGGCGAATCAGAC‑GGGCCGATATTGGCTTGCATAAAGGCGTCTGGCAGGGGTCTGTCTAGGTAACGCCAGAAACGTTTTATTCGAA‑TATCGATCTCGTCTTGTGTTAGAATTCTAACATATTGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGCATGGTCAGCAGGG‑AGAGTGCGTCATCAAGTTCTTTCGCGCCTtccagctcc > 2:433611/5‑251 (MQ=255) gTAATGGGCGAATCAGAC‑GGGCCTATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAA‑TATCGATCTCGTCTTGTGTTAGAATTCTAACATATGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAG‑AGAGTGCGTCATCAAGATCTTTCGCGCCTtccagctccag < 2:368982/251‑1 (MQ=255) gTAATGGGCGAATCAGAC‑GGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGCGGGAACGCCAGAAACGTTTTATTCGAA‑TATCGATCTCGTCTTGTGTTAGAATTCTAACATATGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGGGGTGGTAAAGAATGGTCAGCA‑AGTAGAGTGTGCAATCAAGATATTTCGCGCCTtccagctcc > 2:126843/1‑249 (MQ=255) ggCGAATCAGCC‑GGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAA‑TATCGATCTCGTCTTGTGTTAGAATTCTAACATATGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAG‑AGAGTGCGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTc < 2:141166/251‑1 (MQ=255) gAATCAGAC‑GGGCCGATATTGGCGTGCATAACAGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAA‑TATCGATCTCGTCTTGTGTTAGAATTCTAACATATGGTTGCAACACCGCATACCGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAG‑AGAGTGCGTCTTCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCggg > 2:253358/1‑250 (MQ=255) aC‑GGGTCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGGCGAGGTAACGCCAGAAACGTTTGATTCGAA‑TATCGATCGCGTCTGGTGTTAGAATTCTAACATATGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGGGACCGACGTTACGTGGTGGTAAAGAATGGTCAGCAGAG‑AGAGTGCGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAACCGTTc < 1:460690/251‑1 (MQ=255) c‑GGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAA‑TATCGATCTCGTCTTGTGTTAGAATTCTAACATATGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAG‑AGAGTGCGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAACCGTTCg > 2:437820/1‑251 (MQ=255) c‑GGGCCGAGATTGGCGGGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAA‑TATCGATCTCGTCTTGTGTTAGAATTCTAACATATGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAG‑AGAGTTCGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAACCGTTCg < 1:484520/251‑1 (MQ=255) gATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAA‑TATCGATCTCGTCTTGTGTTAGAATTCTAACATATGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAG‑AGAGTGCGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGa > 2:217085/1‑250 (MQ=255) atTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAA‑TATCGATCTCGTCTTGTGTTAGAATTCTAACATATGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAG‑AGAGTGCGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGAAAAc < 2:382502/251‑1 (MQ=255) atTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAA‑TATCGATCTCGTCTTGTGTTAGAATTCTAACATATGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGGACGTGGTGGTAAAGAATGGTCAGCAGAG‑AGAGTGCGTCATCAAGGTCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGAAAAc > 2:447682/1‑251 (MQ=255) aTAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAA‑TATCGATCTCGTCTTGTGTTAGAATTCTAACATATGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAG‑AGAGTGCGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGAAAACGGGCGTAATc > 2:355909/1‑251 (MQ=255) aaaGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAA‑CATCGATCTCGTCTTGTGTTAGAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGAGGTGGTAAAGAATGGTCAGCAGAG‑AGAGTGCGtcatc < 1:218494/186‑1 (MQ=255) aaaGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAA‑CATCGATCTCGTCTTGTGTTAGAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGAGGTGGTAAAGAATGGTCAGCAGAG‑AGAGTGCGtcatc > 2:218494/1‑186 (MQ=255) cGTCTGGCAGGGTTCTTTCGAGGTAACGCCAGAAACGTTTTATTCGAA‑TATCGATCTCGTCTTGTGTTAGAATTCTAACATATGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAG‑AGAGTGCGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGAAAACGGGCGTAATCGGGTAAg < 2:92542/251‑1 (MQ=255) cTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAA‑TATCGATCTCGTCTTGTGTTAGAATTCTAACATATGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAG‑AGAGTGCGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGAAAACGGGCGTAATCGGGTAAGAc > 1:310449/1‑250 (MQ=255) aGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAA‑TATCGATCTCGTCTTGTGTTAGAATTCTAACATATGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAG‑AGAGTGCGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGCAAACGGGCGTAATCGGGTAAGACATAgcg > 1:334153/1‑251 (MQ=255) aGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAA‑TATCGATCTCGTCTTGTGTTAGAATTCTAACATATGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAG‑AGAGTGCGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGACATTCGGACCCGTTCGCCCGAAAACGGGCGTAATCGGGTAAGACATAgcg > 2:364806/1‑251 (MQ=255) gggTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAA‑TATCGATCTCGTCTTGTGTTAGAATTCTAACATATGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATAGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAG‑AGAGTGCGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGAAAACGGGCGTAATCGGGTAAGACATAgcgc > 1:333485/1‑251 (MQ=255) gTCGAGGTAACGCCAGAAACGTTTTATTCGAA‑TATCGATCTCGTCTTGTGTTAGAATTCTAACATATGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAG‑AGAGTGCGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGAAAACGGGCGTAATCGGGTAAGACATAGCGCGGTTTGt < 1:17951/251‑1 (MQ=255) cGAGGTAACGCCAGAAACGTTTTATTCGAA‑TATCGATCTCGTCTTGTGTTAGAATTCTAACATATGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAG‑AGAGTGCGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGAAAACGGGCGTAATCGGGTAAGACATAGCGCGGTTTGTa < 1:278977/250‑1 (MQ=255) aGGTAACGCCAGAAACGTTTTATTCGAA‑TATCGATCTCGTCTTGTGTTAGAATTCTAACATATGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGCATGGTCAGCAGAG‑AGAGTGCGTCATCAAGATCTTTCGAGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGAAAACGGGCGTAATCGGGTAAGACATAGCGCGGTTTGTACgg > 2:52836/1‑251 (MQ=255) aGAAACGTTTTATTCGAA‑TATCGATCTCGTCTTGTGTTAGAATTCTAACATATGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAG‑AGAGTGCGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCAATTCGGAACCGTTCGCCAGAAAACGGGCGTAATCGGGTAAGACATAGCGCGGTTTGTACGGCGCATGACCt > 1:33220/1‑251 (MQ=255) aaCGTTGTATACGAA‑TATCGCTCTCGTCTTGTGTTAGAATTCTAACATATGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTCCGTGGTGGTAAAGAATGGTCAGCAGAG‑AGAGTGCGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAACCGTTCTCCAGAACACGGGCGTACTCGGGTAAGACATAGCGCGATTTGTACGGCGCAtacccttc > 2:449957/1‑243 (MQ=255) tATTCGAA‑TATCGATCTCGTCTTGTGTTAGAATTCTAACATATGGTTGCAACAACGCATCCATGTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAG‑AGAGTGCGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGAAAACGGGCGTAATCGGGTAAGACATAGCGCGGTTTGTACGGCGCATGACCTTCAAACata < 2:382142/250‑1 (MQ=255) tATTCGAA‑TATCGATCTCGTCTTGTGTTAGAATTCTAACATATGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAG‑AGAGTGCGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGAAAACGGGCGTAATCGGGTAAGACATAGCGCGGTTTGTACGGCGCATGACCTTCACACatat > 1:250892/1‑251 (MQ=255) agtaaaTCGTTCTCGTCTTGTGTTAGAATCCTAACATTTGGTTGTAACAACGGATCCAGGTGCCCCAGGTAGACCGGCATCGATGTGACCGCCGGTACGTGGTGGTAAAGAATGGTCAGCAGAG‑AGAGTGCGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGAAAACGGGCGTAATCGGGTAAGACATAGCGCGGTTTGTACGGCGCATGACCTGCAAACATATCGCa < 2:359225/246‑1 (MQ=255) aatatcgatcgCGTCTTGTGTTAGAATTCTAACATATGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAG‑AGAGTGCGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGAAAACGGGCGTAATCGGGTAAGACATAGCGCGGTTTGTACGGCGCATGACCTTCAAACATATCGCAGa < 1:373201/248‑1 (MQ=255) aataTCGATCTCGTCTTGTGTTAGAATTCTAACATATGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAG‑AGAGTGCGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTAGGAACCGTTCGCCAGAAAACGGGCGTAATCGGGTAAGACATAGCGCGGTTTGTACGGCGCATGACCTTCAAACATATCGc > 2:134592/4‑248 (MQ=255) cATCGCTCTCGTCTTGTGTTAGAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAG‑AGAGTGCGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCTAGAAAACGGGCGTAATCGGGTAAGACATAGCGCGGTTTGTACGGCGCATGACCTTCAAACATATCGCAGAtt > 2:85859/1‑251 (MQ=255) aTCGATCTCGTCTTGTGTTAGAATTCTAACATATGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAG‑AGAGTGCGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAGCCGTTCGCCAGAAAACGGGCGTAATCGGGTAAGACATAGCGCGGTTTGTACGGCGCATGACCTTCAAACATATCGCAGAtt > 1:136338/1‑250 (MQ=255) aTCGATCTCGTCTTGTGTTAGAATTCTAACATATGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAG‑AGAGTGCGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGAAAACGGGCGTAATCGGGTAAGACATAGCGCGGTTTGTACGGCGCATGACCTTCAAACATATCGCAGAtt > 2:25642/1‑250 (MQ=255) cGATCTCGTCTTGTGTTAGAATTCTAACATATGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAG‑AGAGTGCGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGAAAACGGGCGTAATCGGGTAAGACATAGCGCGGTTTGTACGGCGCATGACCTTCAAACATATCGCAGATTac < 1:113852/250‑1 (MQ=255) cGATCTCGTCTTGTGTTAGAATTCTAACATATGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAG‑AGAGTGCGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGAAAACGGGCGTAATCGGGTAAGACATAGCGCGGTTTGTACGGCGCATGACCTTCAAACATATCGCAGATTac < 1:67726/250‑1 (MQ=255) ctcGTCTTGTGTTAGAATTCTAACATATGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAG‑AGAGTGCGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGAAAACGGGCGTAATCGGGTAAGACATAGCGCGGTTTGTACGGCGCATGACCTTCAAACATATCGCAGATTACACCtt > 1:142823/1‑251 (MQ=255) gTCTTGTGTTAGAATTCTAACATATGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAG‑AGAGTGCGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGAAAACGGGCGTAATCGGGTAAGACATAGCGCGGTTTGTACGGCGCATGACCTTCAAACATATCGCAGATTACACCTTCAt > 1:334726/1‑251 (MQ=255) gTCTTGTGTTAGAATTCTAACATATGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAG‑AGAGTGCGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGAAAACGGGCGTAATCGGGTAAGACATAGCGCGGTTTGTACGGCGCATGACCTTCAAACATATCGCAGATTACACCTTCAt > 2:457854/1‑251 (MQ=255) gcTTGTGTTAGAATGCTAAAATATGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAG‑AGAGTGCGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGAAAACGGGCGTAATCGGGTAAGACATAGCGCGGTTTGTACGGCGCATGACCTTCAAACATATCGCAGATTACACCTTCATc < 2:230619/250‑1 (MQ=255) tgtgTTAGAATTCTAACATATGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAG‑AGAGTGCGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAGCCGTTCGCCAGAAAACGGGCGTAATCGGGTAAGACATAGCGCGGTTTGTACGGCGCATGACCTTCAAACATATCGCAGATTACACCTTCATc < 2:136338/248‑1 (MQ=255) tgtgTTAGAATTCTAACATATGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAG‑AGAGTGCGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGAAAACGGGCGTAATCGGGTAAGACATAGCGCGGTTTGTACGGCGCATGACCTTCAAACATATCGCAGATTACACCTTCATc < 1:309527/248‑1 (MQ=255) gtgtTAGAATTCTAACATATGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAG‑AGAGTGCGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGAAAACGGGCGTAATCGGGTAAGACATAGCGCGGTTTGTACGGCGCATGACCTTCAAACATATCGCAGATTACACCTTCATCCAgc > 2:371921/1‑251 (MQ=255) tAGAATTCTAACATATTGGTGCAACAACGCATCCATTGGCCCCAGGTAGACCTGCAGCGAGTTGACCGACTGTTCGTGGTGGTAAAGAATGGTCATCAGAG‑AGAGTGCGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGAAAACGGGCGTAATCGGGTAAGACATAGCGCGGTTTGTACGGCGCATGACCTTCAAACATATCGCAGATTACACCTTCATCCAgcgct < 1:405570/250‑2 (MQ=255) tAGAATTCTAACATATGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAG‑AGAGTGCGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGAAAACGGGCGTAATCGGGTAAGACATAGCGCGGTTTGTACGGCGCATGACCTTCAAACATATCGCAGATTACACCTTCATCCAgcgcg < 1:405577/250‑1 (MQ=255) aTTCTAACATATGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAG‑AGAGTGCGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGAAAACGGGCGTAATCGGGTAAGACATAGCGCGGTTTGTACGGCGCATGACCTTCAAACATATCGCAGATTACACCTTCATCCAg < 1:229309/242‑1 (MQ=255) aTTCTAACATATGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAG‑AGAGTGCGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGAAAACGGGCGTAATCGGGTAAGACATAGCGCGGTTTGTACGGCGCATGACCTTCAAACATATCGCAGATTACACCTTCATCCAg > 2:229309/1‑242 (MQ=255) gcTAACATATGGTTGCAACAACGCCTCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAG‑AGAGTGCGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGAAAACGGGCGTAATCGGGTAAGACATAGCGCGGTTTGTACGGCGCATGACCTTCAAACATATCGCAGATTACACCTTCATCCAGCGCGCGGCggg > 2:422428/2‑251 (MQ=255) tAACATATGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAG‑AGAGTGCGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGAAAACGGGCGTAATCGGGTAAGACATAGCGCGGTTTGTACGGCGCATGACCTTCAAACATATCGCAGATTACACCTTCATCCAGCGCGCGGCGGGCt > 1:324458/1‑251 (MQ=255) aCATATGGTTTCAACACCGCATTCAGTTGTCCAAGGTAAACGGGCAACGATGTGCCCGACGGGACGTGGTGGTAAAGAAGAGCCAGCCGAG‑AGAGTGGGTCATCAAGATCGTTCGCGCCTGCCTGCTCCAGCCATTCGGAACCGTTCGCCAGAAAACGGGCTTAATCGGGTAAGACATATCTCGGTTTGTACGGCGCATGACCTTTTAACATATCGCAGATTACTCCTTCATCCAGCGCGCGGCGGGcgtc < 2:20335/251‑4 (MQ=255) | ATTCACAGATGTTCTTCGCCACTTCCAGCAGCAGGTCATCAGGGGTGATTTCAGGATCGTAGATAAAGGTCAGGTTCGGTGAAACCTGCTTCAACTCTGCATCTGCACGTAAGATCGCGCGGGTAATGGGCGAATCAGAC‑GGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAA‑CATCGATCTCGTCTTGTGTTAGAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAG‑AGAGTGCGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGAAAACGGGCGTAATCGGGTAAGACATAGCGCGGTTTGTACGGCGCATGACCTTCAAACATATCGCAGATTACACCTTCATCCAGCGCGCGGCGGGCTTC > NC_000913/4616435‑4616927 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |