Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,614,170	C→T	F230F (TTC→TTT)	yjjV →	putative metal‑dependent hydrolase YjjV

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,614,170	0	C	T	91.1%	121.7 / NA	47	F230F (TTC→TTT)	yjjV	putative metal‑dependent hydrolase YjjV
Reads supporting (aligned to +/- strand):  ref base C (1/1);  major base T (21/20);  minor base G (0/4);  total (22/25)
Fisher's exact test for biased strand distribution p-value = 1.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACGATTTACCGCGCACTGGCGTGGTTCACGGTTTTTCCGGCAGCCTGCAACAGGCCGAACGGTTTGTACAGCTGGGCTACAAAATTGGCGTAGGCGGTACTATCACCTATCCACGCGCCAGTAAAACCCGCGATGT‑CATCGCAAAATTACCGCTGGCATCGTTATTGCTGGAAACCGACGCGCCGGATATGCCGCTCAACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTCGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCC  >  NC_000913/4613923‑4614408                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                               aCGATTTACCGCGCACTGGCGTGGTTCACGGTTTTTCCGGCAGCCTGCAACAGGCCGAACGGTTTGTACAGCTGGGCTACAAAATTGGCGTAGGCGGTACTATCACCTCTCCACGCGCCAGTAAAACCCGCGATGT‑CATCGCAAAATTACCGCTGGCCTCGTTATTGCTGGAAACCGACGCCCCGGATATGCCGCTCAACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGGTGCCCGTGTGTTTGcc                                                                                                                                                                                                                                             >  2:52042/1‑251 (MQ=255)        aCCGCGCACTGGCGTGGTTCACGGTTTTTCCGGCAGCCTGCAACAGGCCGAACGGTTTGTACAGCTGGGCTACAAAATTGGCGTAGGCGGTACTATCACCTATCCACGCGCCAGTAAAACCCGCGGTGT‑CATCGCAAAATTACCGCTGGCATCGTTATTGCTGGAAACCGACGCGCCGGATATGCCGCTCAACGGTTTTCAGGGGCAGCCTATCCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCttt                                                                                                                                                                                                                                      >  1:11506/1‑251 (MQ=255)        aCCGCGCACTGGCGTGGTTCACGGTTTTTCCGGCAGCCTGCAACAGGCCGAACGGTTTGTACAGCTGGGCTACAAAATTGGCGTAGGCGGTACTATCACCTATCCACGCGCCAGTAAAACCCGCGATGT‑CATCGCAAAATTACCGCTGGCATCGTTATTGCTGGAAACCGACGCGCCGGATATGCCGCTCAACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCttt                                                                                                                                                                                                                                      <  1:255314/251‑1 (MQ=255)        aCCGCGCACTGGCGTGGTTCACGGTTTTTCCGGCAGCCTGCAACAGGCCGAACGGTTTGTACAGCTGGGCTACAAAATTGGCGTAGGCGGTACTATCACCTATCCACGCGCCAGTAAAACCCGCGATGT‑CATCGCAAAATTACCGCTGGCATCGTTATTGCTGGAAACCGACGCGCCGGATATGCCGCTCAACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGGTTGCCGTGCttt                                                                                                                                                                                                                                      >  1:455214/1‑251 (MQ=255)           gcgcACTGGCGTGGTTCACGGTTTTTCCGGCAGCCTGCAACAGGCCGAACGGTTTGTACAGCTGGGCTACAAAATTGGCGTAGGCGGTACTATCACCTATCCACGCGCCAGTAAAACCCGCGATGT‑CATCGCAAAATTACCGCTGGCATCGTTATTGCTGGAAACCGACGCGCCGGATATGCCGCTCAACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCg                                                                                                                                                                                                                                   >  1:370172/1‑251 (MQ=255)             gcACTGGCGTGGTTCACGGTTTTTCCGGCAGCCTGCAACAGGCCGAACGGTTTGTACAGCTGGGCTACAAAATTGGCGTAGGCGGTACTATCACCTATCCACGCGCCAGTAAAACCCGCGATGT‑CATCGCAAAATTACCGCTGGCATCGTTATTGCTGGAAACCGACGCGCCGGATATGCCGCTCAACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCg                                                                                                                                                                                                                                   >  1:245773/1‑249 (MQ=255)                  ggCGTGGTTCACGGTTTTTCCGGCAGCCTGCAACAGGCCGAACGGTTTGTACAGCTGGGCTACAAAATTGGCGTAGGCGGTACTATCACCTATCCACGCGCCAGTAAAACCCGCGATGT‑CATCGCAAAATTACCGCTGGCATCGTTATTGCTGGAAACCGACGCGCCGGATATGCCGCTCAACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTCGCCGTGCTTTGCGAGTTgca                                                                                                                                                                                                                            <  1:247872/251‑2 (MQ=255)                                      cGGCAGCCTGCAACAGGCCGAACGGTTTGTACAGCTGGGCTACAAAATTGGCGTAGGCGGGACTATCACCTATCCACGCGCCAGTAAAACCCGCGATGT‑CATCGCAAAATTACCGCTGGCCTCGTTATTGCTGGAAACCGACGCGCCGGATATGCCGCTCAACGGTTTTCCGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGACCTTTGCGAGTTGCGCCGGGAACCGGCGGATgcgg                                                                                                                                                                                                        >  1:80799/1‑248 (MQ=255)                                       ggCAGCCTGCATCGTGCCTAACGGTTTGTACAGCTGGGCTACAAAATTGGAGTAGGCGGTACTATCCCCTATCCACGCGCCAGTAAAACCCGCGATGT‑CATCGCAAAATTACCGCTGGCACCGTTATTGCGGGAAACGGACGCGCCGGATATGCCGCGCAACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGGTTGCCGTGCTTTGCGAGTTTCGCCGCGAACCGGCGGATGAGAt                                                                                                                                                                                                       <  2:292862/251‑1 (MQ=255)                                                aaccAGGCCGAACGGTTTGTACAGCTGGGCTACAAAATTGGCGTAGGCGGTACTATCACCTATCCACGCGCCAGTAAAACCCGCGATGT‑CATCGCAAAATTACCGCTGGCATCGTTATTGCTGGAAACCGACGCGCCGGATATGCCGCTCAACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGc                                                                                                                                                                                              <  2:238553/248‑1 (MQ=255)                                                    aGGCCGAACGGTTTGTACAGCGGGTCTTCAAAATTGGCGTAGGCGGTACTATCACCTATCCCCGCGCCAGTAAAACCCGCGATGG‑CATCGCAAAATTACCGCTGGCATCGTTATTGCTGGAAACCGACGCGCCGGATATGCCGCTCAACGGGTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGGGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTg                                                                                                                                                                                          <  2:316493/251‑1 (MQ=255)                                                       ccGAACGGTTTGTACAGCTGGGCTACAAAAGTGGCGTAGGCGTTACCATCACCTATCCACGCGCCAGTAAAACCCGCGATGT‑CACCGCAAAATTACCGCTGGCATCGTTATTGCTGGAAACCGACGCGCCGGATATGCCGCTCAACTGTGTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCtt                                                                                                                                                                                       <  2:286621/251‑1 (MQ=255)                                                                           ggCTACAAAATTGGCGTAGGCGGAACTATCACCTATCCACGCGCCAGTAAAACCCGCGATGT‑CATCGCAAAATTACCGCTGGCATCGTTATTGCTGGAAACCGACGCGCCGGATATGCCGCTCAACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACgttgtt                                                                                                                                                                   >  1:47886/1‑251 (MQ=255)                                                                               aCAAAATTGGCGTAGTCGTTACTATCACCTATCCACGCGCCAGTAAAACCCGCGATGT‑CATCGCAAAATTCCCGCTGGCATCGTTATTGCTGGAAACCGACGCGCCGGATATGCCGCTCAACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTa                                                                                                                                                                 <  1:475293/249‑1 (MQ=255)                                                                                      tGGCGTAGGCGGTACTATCACCTATCCACGGGCCAGTAAAACCCGCGAGGT‑CATCGCAAAATTACCGCGGGCATCGTTATTGCTGGAAACCGACGCGCCGGATATGCCGCTCAACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCg                                                                                                                                                         <  1:474926/250‑1 (MQ=255)                                                                                           tAGGCGGTACTATCACCTATCCACGCGCCAGTAAAACCCGCGATGT‑CATCGCAAAATTACCGCTGGCATCGTTATTGCTGGAAACCGACGCGCCGGATATGCCGCTCAACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACAGGCGGATGCGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGcc                                                                                                                                                   >  1:316662/1‑251 (MQ=255)                                                                                                ggTACTATCCCCTACCCACGCGCAAGGAAAACCCGCGATGT‑CATCGCAAAATTACCGCTGGCATCGTTATTGCTGGAAACCGACGCGCCGGATATGCCGCTCAACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCATAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATa                                                                                                                                              <  2:149804/251‑1 (MQ=255)                                                                                                        cACCTATCCACGCGCCAGTAAAACCCGCGATGT‑CATCGCAAAATTACCGCTGGCATCGTTATTGCTGGAAACCGACGCGCCGGATATGCCGCTCAACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTc                                                                                                                                      >  1:9642/1‑251 (MQ=255)                                                                                                                  cgcgCCAGTAAAACCCGCGATGT‑CATCGCAAAATTACCGCTGGCATCGTTATTGCTGGAAACCGACGCGCCGGATATGCCGCTCAACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATc                                                                                                                            >  1:53491/1‑251 (MQ=255)                                                                                                                       cAGTAAAACCCGCGATGT‑CATCGCAAAATTACCGCTGGCATCGTTATTGCTGGAAACCGACGCGCCGGATATGCCGCTCAACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGc                                                                                                                        >  1:162516/1‑250 (MQ=255)                                                                                                                           aaaaCCCGCGA‑GTGCACCGAAAAATTACCGCTGGCATCTTTATTGCTGGAAACCGACGCGCCGGACATGCCGCACAACGTTTTTCAGGGGCGGCCTAACCGCCCGGAGCAGGCTGCCCGTGGGTTGGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTg                                                                                                                   <  2:230370/251‑1 (MQ=255)                                                                                                                            aaaaccaggcaagacaactcAAAATTCCCGCTTGAAACGTTATGGCGGGAAACCGAGGCGCGGGAGAGGCCGCTCAACGGGTGGCAGGGGCAGCCTAACCGCCGGGAGCAGGCTGCCGAGGTGTTGGCCGTGCTTGGCGAGTGGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTAAATAACGCTTATACGTTGTTTAACGTGCCGTAGGCCGGATAGGGCGTTCCCGCCGCATCCGGCAGtt                                                                                                                    <  2:241318/231‑1 (MQ=255)                                                                                                                                ccGCGATGT‑CATCGAAAAATTCCCGCTGGCATCGTTATTGTTGGAATCCGACGCGCCGGATATGCCGCTCAACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTGGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGca                                                                                                              <  2:281818/251‑1 (MQ=255)                                                                                                                                    gATGT‑CAGCGCAAAATTACCGCTGGCTTCGTTAATGCTGGAAACCGACGCGCCGGATATGCCGCTCAACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCCCAAGCGGTGCTTAATAACACGTATACGTTGTTTAACGGGCCGTAGGCCGGATAAGGGGTTCCCGCAGCAACCGGccatgtgcgccc                                                                                                             >  2:373403/1‑237 (MQ=255)                                                                                                                                      tGT‑CATCGCAAAATTACCGCTGGCATCGTTATTGCTGGAAACCGACGCGCCGGATATGCCGCTCAACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGGGATTGCGCCAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGAAAAGGCGTTCACGCCGCATCCGGCAGtttgagcaccgtgc                                                                                                        >  2:173067/1‑239 (MQ=255)                                                                                                                                             cGCAAAATTACCGCTGGCATCGTTATTGCTGGAAACCGACGCGCCGGATATGCCGCTCAACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCACGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGAGTTCACGCCGCATCCGGCCGTTGGCGCACAATGCCTGATg                                                                                                  >  1:284299/1‑251 (MQ=255)                                                                                                                                                     tcacGCTGGAATCGTTATTGCTGGAACCCTCCGCGCCGGATATGCCGCTCAACGGTTTTCAGGGGCAGCCTAACCTCCCGGAGCAGGCTGCCGGTGTGTTTGCCGTGCTTGGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGGGCCGTAGGCCGGATAAGCCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCt                                                                                           <  2:80799/247‑1 (MQ=38)                                                                                                                                                      aCCGCTGGCGTCGTTATTGCTGGAAACCGACGCGCCGGATATGCCGCTCAACGGTTTTCAGGGGCAGACTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGACCCGGCGGATGAGGTTGCGCACGCTTTTCTTAATCACACTTTTACGGTGGTTTACGTTCCGTAGGCCGGATAAGGCGTACACGCCACATCCGTCAGTTggacctcaatcccagtatggcgccatt                                                                                         >  2:37526/1‑226 (MQ=255)                                                                                                                                                      aCCGCTGGCATCGTTATTGCTGGAAACCGACGCGCCGGATATGCCGCTCAACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTa                                                                                         <  1:465674/251‑1 (MQ=255)                                                                                                                                                       ccGCTGGCATCGTTATTGCTGGAAACCGACGCGCCGGATATGCCGCTCAACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTTATGCGACGCTTaa                                                                                        <  1:141868/251‑1 (MQ=255)                                                                                                                                                               aTCGTTATTGCTGGGAACCGACGCGCCGGATATGCCGCTCAACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTCGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCCCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCtt                                                                                >  1:402530/1‑251 (MQ=255)                                                                                                                                                               aTCGTTATTGCTGGAAACCGACGCGCCGGATATGCCGCTCAACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACCCGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGGGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCtt                                                                                >  2:413394/1‑251 (MQ=255)                                                                                                                                                                 gtttATTGCTGGAACCCGACGCGCCGGATTGGCCTCTCAACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTa                                                                               <  1:284688/248‑1 (MQ=255)                                                                                                                                                                                ccgccgggcCGGATATGCCGTTCAACGGTTTCCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGttt                                                               <  2:392248/244‑1 (MQ=255)                                                                                                                                                                                 cGACGCGCCGGATAGGCCGCTCAACGGTTTGCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTtg                                                              <  1:114820/251‑1 (MQ=255)                                                                                                                                                                                      ctccttatcttccGCTCAACGTTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACCCGTATACGTTGTTTAACGAGCCGTAGGCCGCATAAGGCGTTCACGCCGCCTCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTTTGCCg                                                         >  1:213951/12‑251 (MQ=38)                                                                                                                                                                                              atGCCGCTCACCGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAg                                                 <  1:102247/251‑1 (MQ=255)                                                                                                                                                                                                  cGCTCAACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGGGTTCACGCAGCATCCGGCAGATGGCGCCCAATTCCTGGTGCGACGCTTAACGCGGCTTCTCATGCCTCCAGGTTTGTGCCGAACCGTAGGCCg                                             >  2:493729/1‑251 (MQ=255)                                                                                                                                                                                                            ttgtcgggggCGGCCTAACCGCCCGGAGCGGGCGGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATGGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGGGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCg                                    <  2:255525/244‑1 (MQ=25)                                                                                                                                                                                                               tCAGGGGCAGCCTAACCGCCCGGCGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCCCAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGGAGGCCGGGTAAGGCGTTcc                                >  2:795/1‑250 (MQ=255)                                                                                                                                                                                                                       gccctacccgccgggggaaggctcgccGTGGGTTGGCCGTGCTGTGCGTGGTGCGCCGGGACCCGGCGGATGGGATTGCGTAAGCGTTGTTTAATATCTCGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCTTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACATGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCAt                        <  2:199045/226‑1 (MQ=18)                                                                                                                                                                                                                              ccGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGc                   >  1:493333/1‑249 (MQ=255)                                                                                                                                                                                                                                gCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGtt               >  1:362910/1‑251 (MQ=255)                                                                                                                                                                                                                                 cccGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTg              <  1:187737/251‑1 (MQ=255)                                                                                                                                                                                                                                 cccGGAGCAGGCTGCCCGTGTGTTTGCCGGGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTg              <  1:373688/251‑1 (MQ=255)                                                                                                                                                                                                                                   cgtagcaggcgtcctgtgtgtTGGCCGTGCTTTGCGAGTGGGTCGGGGACCCGGGGGGTGGGATTGCGCAAGTGTTGCTTAATAACCGGTATACTTTGTTTAGCGGGCGGAAGGCCGGAGAAGGCGTTCACGCCGCATCTGGCAGTTGGCGCACAATGCCTGATGCGAGGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGGCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGgc            <  2:47886/236‑1 (MQ=12)                                                                                                                                                                                                                                             ggCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGAAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGcc  <  1:98739/250‑1 (MQ=255)                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                               ACGATTTACCGCGCACTGGCGTGGTTCACGGTTTTTCCGGCAGCCTGCAACAGGCCGAACGGTTTGTACAGCTGGGCTACAAAATTGGCGTAGGCGGTACTATCACCTATCCACGCGCCAGTAAAACCCGCGATGT‑CATCGCAAAATTACCGCTGGCATCGTTATTGCTGGAAACCGACGCGCCGGATATGCCGCTCAACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTCGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCC  >  NC_000913/4613923‑4614408

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑