| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,560,309 | T→A | P124P (CCA→CCT) | yjiH ← | uncharacterized protein YjiH |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,560,309 | 0 | T | A | 91.7% | 98.9 / ‑1.7 | 36 | P124P (CCA→CCT) | yjiH | uncharacterized protein YjiH |
| Reads supporting (aligned to +/- strand): ref base T (1/2); new base A (14/19); total (15/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.22e-01 | |||||||||||
GACAAATTTAAACACCAGGATGACGGCTAAAGGTACGATTACGGACGTGCCCAGAAAGGCAAACACCGCGACGCCGGAAGAGAAGTAGTTGGTGATGATGGCGCTACCGCTGGTTTGATATGCGGCGAAAATCACTTTATCGCGTTCGGTAATTTCACCTTCCTGCGCCAGCTCTTTGGTCATACCGGCGGCGGCATCGGTGTTTTGCAGGTTAGCGATCAGCGCCAGCGAGCAGATCCCTGGAATGCCCAACAACGGTTTTAGCACAGGTGT‑CATTAACTGCTGCGCGGCGCGTAATCCACCAAGACCATCGGTAATTGAAATAATCCCCAGAGAGAGAATGACTGACGGTGCCAGCTCCAGCGCAAAGAGAAAGCCATCTTTAGCCCCCGCGCCACCCGCGCCGCGAAATGAGGTGGTGGCTCCGTTCGCACCAGGCAGTTGCCCAAACGATCCGTTCAGAACGGAAAAATCAAAAACGCGC > NC_000913/4560069‑4560552 | gACAAATTTAAACACCAGGATGACGGCTAAAGGTACGATTACGGACGTGCCCAGAAAGGCAAACACCGCGACGCCGGAAGAGAAGTAGTTGGTGATGATGGCGCTACCGCTGGTTTGATATGCGGCGAAAATCACTTTATCGCGTTCGGTAATTTCACCTTCCTGCGCCAGCTCTTTGGTCATACCGGCGGCGGCATCGGTGTTTTGCAGGTTAGCGATCAGCGCCAGCGAGCAGATCCCAGGAATGCCca < 1:168147/251‑1 (MQ=255) aaaCACCAGGATGACGGCTAAAGGTACGATTACGGACGTGCCCAGAAAGGCAAACACCGCGACGCCGGAAGAGAAGTAGTTGGTGATGATGGCGCTACCGCTGGTTTGATATGCGGCGAAAATCACTTTATCGCGTTCGGTAATTTCACCTTCCTGCGCCAGCTCTTTGGTCATACCGGCGGCGGCATCGGTGTTTTGCAGGTTAGCGATCAGCGCCAGCGATCAGATCCCAGGAATGCCCAACAACGGt < 2:322594/250‑1 (MQ=255) gATGACGGCTAAAGGTACGATTACGGACGTGCCCAGAAAGGCAAACACCGCGACGCCGGAAGAGAAGTAGTTGGTGATGATGGCGCTACCGCTGGTTTGATATGCGGCGAAAATCACTTTATCGCGTTCGGTAATTTCACCTTCCTGCGCCAGCTCTTTGGTCATACCGGCGGCGGCATCGGTGTTTTGCAGGTTAGCGATCAGCGCCAGCGAGCAGATCCCAGGAATGCCCAACAACGGTTTTAGcac > 1:44010/1‑249 (MQ=255) gatggCTAAAGGTACGATTACGGACGTGCCCAGAACGGCAAACAACGCGACGCCGGACGAGAAGGAGTTGGTGATGATGGCGCTACCGCTGGATTGATATGCGGCGAAAATCACTTTATCGCGATCGGGAATTTCATCTTCCTGCGCCAGCTCTTTGGACATACCGGAGGCGGCATCGGTGTTTTGCAGGTTAGCGATCAGCGCAAGCGAGCAGATCCCAGGGATGGCCAACAACGATTTTAGCACAGgtg > 1:400330/4‑251 (MQ=255) aaGGTACGATTACGGACGGGCCCAGAAAGGCAAACCCCGCGACGCCGGAAGAGAAGTAGTTGGTGATGATGGCGCTACCGCTGGTTTGATATGCGGCGAAAATCACTTTATCGCGTTCGGTAATTTCACCTTCCTGCGCCAGCTCTTTGGTCATACCGGCGGCGGCATCGGTGTTTTGCAGGTTAGCGATCAGCGCCAGCGAGCAGATCCCAGGAATGCCCAACAACGGTTTTAGCACAGGTGT‑CATTAAc < 1:334647/251‑1 (MQ=255) gTGCCCAGAAAGGCAAACACCGCGACGCCGGAAGAGAAGTAGTTGGTGATGATGGCGCTACCGCTGGTTTGATATGCGGCGAAAATCACTTTATCGCGTTCGGTAATTTCACCTTCCTGCGCCAGCTCTTTGGTCATACCGGCGGCGGCATCGGTGTTTTGCAGGTTAGCGATCAGCGCCAGCGAGCAGATCCCAGGAATGCCCAACAACGGTTTTAGCACAGGTGT‑CATTAACTGCTGCGCGGCGCGTaa < 1:291977/251‑1 (MQ=255) aaaCACCGCGACGCCGGAAGAGAAGTAGTTGGTGATGATGGCGCTACCGCTGGTTTGATATGCGGCGAAAATCACTTTATCGCGTTCGGTAATTTCACCTTCCTGCGCCAGCTCTTTGGTCATACCGGCGGCGGCATCGGTGTTTTGCAGGTTAGCGATCAGCGCCAGCGAGCAGATCCCAGGAATGCCCAACAACGGTTTTAGCACAGGTGT‑CATTAACTGCTGCGCGGCGCGTAATCCACCAAGACCAt < 1:147949/251‑1 (MQ=255) cgcgACGCCGGAAGAGAAGTAGTTGGTGATGATGGCGCTACCGCTGGTTTGATATGCGGCGAAAATCACTTTATCGCGTTCGGTAATTTCACCTTCCTGCGCCAGCTCTTTGGTCATACCGGCGGCGGCATCGGAGTTTTGCAGGTTAGCGATCAGCGCCAGCGAGCAGATCCCAGGAATGCCCAACAACGGTTTTAGCACAGGTGT‑CATTAACTGCTGCGCGGCGCGTAATCCACCAAGACCATCGGTa > 1:200784/1‑250 (MQ=255) aCGCCGGAAGAGAAGTAGTTGGTGATGATGGCGCTACCGCTGGTTTGATATGCGGCGAAAATCACTTTATCGCGTTCGGTAATTTCACCTTCCTGCGCCAGCTCTTTGGTCATACCGGCGGCGGCATCGGAGTTTTGCAGGTTAGCGATCATCGCCAGCGAGCAGATCCCAGGAATGCCCAACAACGGTTTTAGCACAGGTTT‑CATTAACTGCTGCGCGGCGCGTAATCAACCAAGACAACCAGTAATTg > 2:110669/1‑250 (MQ=255) gAAGAGATGTAGTTGTTGATGAGGGCGCTTCCGCTTGTTGGATATGCGGCGTAAATCACTTTATCGCGTTCGGTAATTTCACCTTCCTGCGCCAGCTCTTTGGTCATACCGGCGGCGGCATCGGTGTTTTGCAGGTTAGCGATCAGCGCCAGCGAGCAGATCCCAGGAATGCCCAACAACGGTTTTAGCACAGGTTT‑CATTAACTGCTGCGCGGCGCGTAATCCACCAAGACCCTCGGTAATGGAaataat < 2:21076/251‑1 (MQ=255) gagaAGTAGTTGGTGATGATGGCGCTACCGCTGGTTTGATATGCGGCGAAAATCACTTTATCGCGTTCGGTAATTTCACCTTCCTGCGCCAGCTCTTTGGTCATACCGGCGGCGGCATCGGTGTTTTGCAGGTTAGCGATCAGCGCCAGCGAGCAGATCCCAGGAATGCCCAACAACGGTTTTAGCACAGGTGT‑CATTAACTGCTGCGCGGCGCGTAATCCACCAAGACCATCGGTAATTGAAATAATccc < 1:274467/251‑1 (MQ=255) agtTGGTGATGATGTCGCTACCGCTGGTTTGATATGCGGCGAAAATCCCTTTTTCGCGGTCGGTAATTTCTCCTTCCTGCGCCAGCTCTTTGGTCATACCGGCGGCGGCATCGGTGTTTTGTAGGTTAGCTATCAGCGCCAGCGAGCAGATCCCTGGAATGCCCAACAACGGTTTTAGCACAGGTGT‑CATTAACTGCGGCGCGGCGCGTAATCCACCAAGACCATCGGTAATTGAAATAATCCCCagaga < 1:37210/250‑1 (MQ=255) gTGATGATGGCGCTACCGCTGGTTTGATATGCGGCGAAAATCTCTTTATCGCGTTCGGTAATTTCACCTTCCTGCGCCAGCTCTTTGGTCATACCGGCGGCGGCATCGGTGTTTTGCAGGTTAGCGATCAGCGCCAGCGAGCAGATCCCAGGAATGCCCAACAACGGTTTTAGCACAGGTGT‑CATTAACTGCTGCGCGGCGCGTAATCCACCAAGACCATCGGTAATTGAAATAATCCCCAGAGAGAGAAt < 1:387132/251‑1 (MQ=255) gatgGCGCTACCGCTGGTTTGATATGCGGCGAAAATCCCTTTATCGCGTTCGGTAATTTCACCTTCCTGCGCCAGCTCTTTGGTCATACCGGCGGCGGCATCGGTGTTTTGCAGGTTAGCGATCAGCGCCAGCGAGCAGATCCCAGGAATGCCCAACAACGGTTTTAGCACAGGTGT‑CATTAACTGCTGCGCGGCGCGTAATCCACCAAGACCATCGGTAATTGAAATAATCCCCAGAGAGAGAAtgactg < 2:143855/251‑1 (MQ=255) ggCGCTACCGCTGGTTTGATATGCGGCGAAAATCACTTTATCGCGTTCGGTAATTTCACCTTCCTGCGCCAGCTCTTTGGTCATACCGGCGGCGGCATCGGTGTTTTGCAGGTTAGCGATCAGCGCCAGCGAGCAGATCCCAGGAATGCCCAACAACGGTTTTAGCCCAGGTGT‑CATTAACTGCTGCGCGGCGCGTAATCCACCAAGACCATCGGTAATTGAAATAATCCCCAGAGAGAGAATGACTGACg > 2:271283/1‑251 (MQ=255) tACCGCTGGTTTGATATGCGGCGAAAATCACTTTATCGCGTTCGGTAATTTCACCTTCCTGCGCCAGCTCTTTGGTCATACCGGCGGCGGCATCGGTGTTTTGCAGGTTAGCGATCAGCGCCAGCGAGCAGATCCCAGGAATGCCCAACAACGGTTTTAGCACAGGTGT‑CATTAACTGCTGCGCGGCGCGTAATCCACCAAGAACATCGGTAATTGAAATAATCCCCAGAGAGAGAATGACTGACGGTGcc > 1:131614/1‑251 (MQ=255) tACCGCTGGTTTGATATGCGGCGAAAATCACTTTATCGCGTTCGGTAATTTCACCTTCCTGCGCCAGCTCTTTGGTCATACCGGCGGAGGCATCGGTGTTTTGCCGGTTAGCGATCCGCGCCAGCCAGCAGATCCCAGGAATGCCCAACAACGGTTTTAGCACAGGTGT‑CATTAACTGCTGAGCAGCGCGTAATCCACCAAAACCATCGGTAATTGAAATAATCACCAGAGAGAGAATGACTGACggagac > 1:484564/1‑247 (MQ=255) ccGCTGGTTTGATATGCGGCGAAAATCGCTTTATCGCGTTCGGTAATTTCGCCTTCCTGCGCCAGCTCTTTGGTCATACCGGCGGCGGCAGCGGTGTTTTGCAGGTTAGCGATCAGCGCCAGCGAGCAGATCCCAGGAATGCTCAACAACGGTTTTAGCACAGGTGT‑CATTAACTGCTGCGCGGCGCGTAATCCACCAAGACCACCGGTAATTGAAATAATCCCCAGAGAGAGAATGACTGACGGTGCCAg < 2:344032/251‑1 (MQ=255) ccGCTGGTTTGATATGCGGCGAAAATCACTTTATCGCGTTCGGTAATTTCACCTTCCTGCGCCAGCTCTTTGGTCATACCGGCGGCGGCATCGGTGTTTTGCAGGTTAGCGATCAGCGCCAGCGAGAAGATCCCAGGAATGCCCAACAACGGTTTTAGCACAGGTGT‑CATTAACTGCTGCGCGGCGGGTAATCCACCAAGACCATCGGTAATTGAAATAAACCCCAGAGAGAGAATGACTGACGGTGcc > 1:261456/1‑249 (MQ=255) tcgATATGCGGCGAAAATCACTTTATCGCGTTCGGTAATTTCACCTTCCTGCGCCAGCTCTTTGGTCATACCGGCGGCGGCATCGGTGTTTTGCAGGTTAGCGATCAGCGCCAGCGAGCAGATCCCTGGAATGCCCAACAACGGTTT‑AGCACAGGTGT‑CATTAACTGCTGCGCGGCGCGTAATCCACCAAGACCATCGGTAATTGAAATAATCCCCAGAGAGAGAATGACTGACGGTGCCAGCTCCAgcg < 2:152169/248‑1 (MQ=255) cTTTATCGCGTTCGGTAATTTCACCTTCCTGCGCCAGCTCTTTGGTCATACCGGCGGCGGCATCGGTGTTTTGCAGGTTAGCGATCAGCGCCAGCGAGCAGATCCCAGGAATGCCCAACAACGGTTTTAGCACAGGTGT‑CATTAACTGCTGCGCGGCGCGTAATCCACCAAGACCATCGGt < 1:108932/181‑1 (MQ=255) cTTTATCGCGTTCGGTAATTTCACCTTCCTGCGCCAGCTCTTTGGTCATACCGGCGGCGGCATCGGTGTTTTGCAGGTTAGCGATCAGCGCCAGCGAGCAGATCCCAGGAATGCCCAACAACGGTTTTAGCACAGGTGT‑CATTAACTGCTGCGCGGCGCGTAATCCACCAAGACCATCGGt > 2:108932/1‑181 (MQ=255) gTTCGGTAATTTCACCTTCCTGCGCCAGCTCTTTGGTCATACCGGCGGCGGCATCGGTGTTTTGCAGGTTAGCGATCAGCGCCAGCGAGCAGATCCCAGGAATGCCCAACAACGGTTTTAGCACAGGTGT‑CATTAACTGCTGCGCGGCGCGTAATCCACCAAGACCATCGGTAATTGAAATAATCCCCAGAGAGAGAATGACTGACGGTGCCAGCTCCAGCGCAAAGAGAAAGCCATCTTTAGCCCCcgcg > 1:414866/1‑251 (MQ=255) ccTTCATGCGCCAGCTCTTTGGTCATACCGGCGGCGGCATCGGTGTTTTGCAGGTTAGCGAGCAGCGCCAGCGGGCAGATGCCTGGAATGACCAACAACGGTTTTAGCACAGGTGT‑CATTAACAGCTGCGCGGCGCGTAATACACCAAGACCATCGGTAGTTGAAGTAATCCCCAGAGAGAGAATGACTACCGGTGCTGGCTCCAGCGCACACAGAAAGCCATCTTTAGCCCCCGCGCCACccgcaccgcg > 1:76701/1‑251 (MQ=255) cgcCAGCTCTTTGGTCATACCGGCGGCGGCATCGGTGTTTTGCAGGTTAGCGATCAGCGCCAGCGAGCAGATCCCAGGAATGCCCAACAACGGTTTTAGCACAGGTGT‑CATTAACTGCTGCGCGGCGCGTAATCCACCAAGACCATCGGTAATTGAAATAATCCCCAGAGAGAGAATGACTGACGGTGCCAGCTCCAGCGCAAAGAGAAAGCCATCTTTAGCCCCCGCGCCACCCGCGCCGCGAAATGAgg > 1:353642/1‑251 (MQ=255) ttGGTCATACCGGCGGCGGCATCGGTGTTTTGCAGGTTAGCGATCAGCGCCAGCGAGCAGATCCCAGGAATGCCCAACAACGGTTTTAGCACAGGTGT‑CATTAACTGCTGCGCGGCGCGTAATCCACCAAGACCATCGGTAATTGAAATAATCCCCAGAGAGAGAATGACTGACGGTGCCAGCTCCAGCGCAAAGAGAAAGCCATCTTTAGCCCCCGCGCCACCCGCGCCGCGAAATGAGGTGGTGGCTc < 1:271283/250‑1 (MQ=255) cggccgctgagtTTTGCATGTTGGCGATCATCGCCTGCGATCAGATCCCAGGAATGCTCAACCACGGTTGTAGCACAGGTGT‑CATTAACTGCTGCGCGGCGCGTAATCCACCAAGACCATCGGTAATTGAAATAATCCCCTGAGAGAGAATGACTGACGGTGCCACCTCCAGCGCAAAGAGAAAGCCATCTTTAGCCCCCGCGCCTCCCGCGCCGCGAAATGTGGTGGTTGCTCCGTTCGCATCAGGCAGt < 2:331864/241‑1 (MQ=255) ggtttGCAGGTGATCGATCAGCGACAGTGAGCATGTCCCAGGAAAGCCCAACAACGGGTTTAGCACATGTGT‑CATTAACTGCTGCGCGGCGCGTAACCCACCAAGACCATCGGTAATTGAAATAATCCCCAGAGAGAGAATGACTGACGGTGCCAGCTCCAGCGCAAAGAGAAAGCCATCTTTAGCCCCCGCGCCACCCGCGCCGCGAAATGAGGTGGTGGCTCCGTTCGCACCAGTCAGTTGCCCAAAct < 2:348010/249‑2 (MQ=255) tggcatgTTAGCGATCAGCGAAAGCGAGCAGATCCCAGGAATGCCCAACAACGGTTTTAGCACAG‑TGTGCATTAACTGCTGCGCGGCGCGTAATCCACCAAGACCAACGGGAATTGAAATAATCCCCAGAGAGAGAATGACTGACGGGTCCAGCTCCAGCGCAACGAGAAAGCCATCTTTAGCCCCTGCGCCGCCCTCGCCGCGAAATGAGTGGGAGGCTCCGTTCGCCCAAGGCAGTTGCCCAAACGAt < 2:246146/244‑1 (MQ=255) gTTATCGATCAGCGCCAGCGAGCCGATCCCAGGAATGCCCAACAACGGTTTTAGCACAGGTGT‑CATTAACTGCTGCGCGGCGCGTAATCCACCAAGACCATCGGTAATTGAAATAATCCCCAGAGAGAGAATGACTGACGGTGCCAGCTCCAGCGCAAAGAGAAAGCCATCTTTAGCCCCCTCGCCACCCGCGCCGCGAAATGAGGTGGTGGCTCCGTTCGCACCAGGCAGTTGCCCAAACGATCCGTTCa < 2:466184/251‑1 (MQ=255) tAGCGATCAGCGCCAGCGAGCAGATCCCAGGAATGCCCAACAACGGTTTTAGCACAGGTGT‑CATTAACTGCTGCGCGGCGCGTAATCCACCAGGACCATCGGTAATTGAAATAATCCCCAGAGAGAGAATGACTGACGGTGCCAGCTCCAGCGCAAAGAGAAAGCCATCTTTAGCCCCCGCGCCACCCGCGCCGCGAAATGAGGGGGTGGCTCCGTTCGCACCATGCAGTTGCCCAACCGATCCGTTc > 1:384065/1‑248 (MQ=255) aTCAGCGCCAGTGAGCAGATCCCAGGAATGCCCAACAACGGTTTTAGCACAGGTGT‑CATTAACTGCTGCGCGGCGCGTAATCCACCAAGACCATCGGTAATTGAAATAATCCCCAGAGAGAGAATGACTGACGGTGCCAGCTCCAGCGCAAAGAGCAAGCCATCTTTAGCCCCCGCGCCACCCGCGCCGCGAAATGAGGAGGTGGCTCCGTTCGCACCAGGCAGTTGACCaacgcacccgttcaaaaagg > 2:328479/1‑232 (MQ=255) cAGCGCCAGCGAGCAGATCCCAGGAATACCCAACAACGGTTTTAGCACAGGTGT‑CATTAACTGCTGCGCGGCGCGTAATCCACCAAGACCATCGGTAATTGAAATAATCCCCAGAGAGAGAATGACTGACGGTGCCAGCTCCAGCGCAAAGAGAAAGCCATCTTTAGCCCCCGCGCCACCCGCGCCGCGAAATGAGGTGGTGGCTCCGTTCGCACCAGGCAGTTGCCCAAACGATCCGTTCAGAACGGaaa < 1:344183/251‑1 (MQ=255) ccAGCGAGCAGATCCCAGGAATGCCCAACAACGGTTTTAGCACAGGTGT‑CATTAACTGCTGCGCGGCGCGTAATCCACCAAGACCATCGGTAATTGAAATAATCCCCAGAGAGAGAATGACTGACGGTGCCAGCTCCAGCGCAAAGAGAAAGCCATCTTTAGCCCCCGCGCCACCCGCGCCGCGAAATGAGGTGGTGGCTCCGTTCGCACCAGGCAGTTGCCCAAACGATCCGTTCAGAACGGAAAAATCa < 1:435264/251‑1 (MQ=255) aGCGAGCAGATCCCAGGAATGCCCAACAACGGTTTTAGCACAGGTGT‑CATTAACTGCTGCGCGGCGCGTAATCCACCAAGACCATCGGTAATTGAAATAATCCCCAGAGAGAGAATGACTGACGGTGCCAGCTCCAGCGCAAAGAGAAAGCCATCTTTAGCCCCCGCGCCACCCGCGCCGCGAAATGAGGTGGTGGCTCCGTTCGCACCAGGCAGTTGCCCAAACGATCCGTTCAGAACGGAAAAATCaaa < 1:49170/251‑1 (MQ=255) aGATCCCAGGAATGCCCAACAACGGTTTTAGCACAGGTGT‑CATTAACTGCTGCGCGGCGCGTAATCCACCAAGACCATCGGTAATTGAAATAATCCCCAGAGAGAGAATGACTGACGGTGCCAGCTCCAGCGCAAAGAGAAAGCCATCTTTAGCCCCCGCGCCACCCGCGCCGCGAAATGAGGTGGTGGCTCCGTTCGCACCAGGCAGTTGCCCAAACGATCCGTTCAGAACGGAAAAATCAAAAACgcgc > 1:372150/1‑251 (MQ=255) | GACAAATTTAAACACCAGGATGACGGCTAAAGGTACGATTACGGACGTGCCCAGAAAGGCAAACACCGCGACGCCGGAAGAGAAGTAGTTGGTGATGATGGCGCTACCGCTGGTTTGATATGCGGCGAAAATCACTTTATCGCGTTCGGTAATTTCACCTTCCTGCGCCAGCTCTTTGGTCATACCGGCGGCGGCATCGGTGTTTTGCAGGTTAGCGATCAGCGCCAGCGAGCAGATCCCTGGAATGCCCAACAACGGTTTTAGCACAGGTGT‑CATTAACTGCTGCGCGGCGCGTAATCCACCAAGACCATCGGTAATTGAAATAATCCCCAGAGAGAGAATGACTGACGGTGCCAGCTCCAGCGCAAAGAGAAAGCCATCTTTAGCCCCCGCGCCACCCGCGCCGCGAAATGAGGTGGTGGCTCCGTTCGCACCAGGCAGTTGCCCAAACGATCCGTTCAGAACGGAAAAATCAAAAACGCGC > NC_000913/4560069‑4560552 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |