Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,556,917	A→G	E2G (GAA→GGA)	idlP →	iraD leader peptide

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,556,917	0	A	G	92.9%	110.0 / ‑1.3	42	E2G (GAA→GGA)	idlP	iraD leader peptide
Reads supporting (aligned to +/- strand):  ref base A (2/1);  new base G (22/17);  total (24/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.31e-01

CCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGAAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGC‑ATATGTTGCTAAACGTCAGGAGTGCGCAAAATGATGCGACAATCACTTCAGGCTGTTTTACCTGAAATTTCAGGCAATAAAACGTCATCGCTGCGTAAATCGGTATGCAGCGATCTCCTCACTCTTTTTAATTCTCCTCATTCGGCATTACCGTCATTACTTGTTTCAGGCATGCCCGAGTGGCAAGTGCATAACCCGAGC  >  NC_000913/4556687‑4557163                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                        ccGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGAATGACATGGATGTAATTAACAAAAGGGAATGGATTTGGGAAATGAGCATCAATACAggg                                                                                                                                                                                                                                     >  1:506226/1‑249 (MQ=255)     acttAAGTAGCAGCGGAGACAAGTATTTTCATCTACGTAACTATTCGGAAAATTCAGAATATACTCGCGGTTTTTTTTTGAGTTTGATGGTTTTTATCAAATCATGACTTTTTTCCTGAAAAGTTAGTAGATATTCATTAGCAAGTAAGGATTTATACTTTGCTTTCATGTTTCAGACCTGTATCAGACTGTCATGGATGGAATTAACAAAGGGGAATGGCCATGGGACATGAGCAACAATACAGTTGTGc                                                                                                                                                                                                                                 >  2:144792/3‑251 (MQ=255)               cagcGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGAAAATGAGCATCAATACAGTGGTGCCCGGTGTTc                                                                                                                                                                                                                        >  1:440982/1‑250 (MQ=255)                          aGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCCTCAATAAAGTGGTGCCCGGTGCTCAGGGCAcgccgc                                                                                                                                                                                                            >  1:471677/1‑251 (MQ=255)                                                      atatTCAGAATATACTAGCGGTTTTTTTTTGAGTTTGATTATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTTCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGACCAAAAGGGAATGGCTATGGGAAATGGGCATCAATACAGTGTGGCCCGGTGTTCAGGGCAAGCCGA‑ATATGTTTCTAAACGTCAGGAGTgcgc                                                                                                                                                                                >  2:173550/1‑250 (MQ=255)                                                        atTCAGAATATACTAGCGGTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGAAATGGATGTAATGAACAAAAGGGAAAGGCTATGGAAAATGAGACTCCATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGC‑ATATGTTGCTAAACGGCAGGAGTgcgaaa                                                                                                                                                                              >  2:233250/1‑247 (MQ=255)                                                              aaTATATTAGCGGTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGC‑ATATGTTGCTAAACGTCAGGAGTGCGCAAAatgatg                                                                                                                                                                       <  2:158312/251‑1 (MQ=255)                                                              aaTATACTAGCGGTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGC‑ATATGTTGCTAAACGTCAGGAGTGAGCAAAatgatg                                                                                                                                                                       >  1:475369/1‑251 (MQ=255)                                                                tataCTAGCGGTATTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTTAAAAGTCAGTAGGTATTAATTGGCAAGTAAGGTTTTATACTTTGAAGACAGGATTCAAGGCTGTCTCAGACTGACCTGGATGTAATGCACAAAAGGGAATGGCTATGGGAAATGAGCTTCACTTCCGTGGTGCCCGGGGTTCAGGGCAAACCGCCAT‑TGTTGCTAAAAGAGAGGAGGGCGCAAAatgagacg                                                                                                                                                                     >  2:55704/1‑247 (MQ=255)                                                                 ataCTAGCGGTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAGTGGTGCCCGGTGGTCAGGGCAAGCCGC‑ATATGTTGCTAAACGTCAGGAGTGCGCAAAATGATGCg                                                                                                                                                                     <  1:249247/250‑1 (MQ=255)                                                                   aCTAGCGGTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAAGCAAGTACAGTTTTATACTTTGCTGACCGGATTCAGGCCTGTCTCAGACTGCCATGGATGTAATGAACAAAAGGGAATGGGTATGGGAAATGAGCATCAATAAAATGGTGCCCGGGGTTCAGGGCAAGCCGA‑ATATGTTGCTAAACGTCAGGAGTGCGCAAAAAGATGCGAc                                                                                                                                                                   >  1:460395/1‑250 (MQ=255)                                                                     tAGCGGTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAGTGGGGCCCGGTGTTCAGGGCAAGCAGC‑ATATGTTGCTAAAAGTCAGGAGTGCGCAAAATGATGCGACAAt                                                                                                                                                                >  1:366040/1‑251 (MQ=255)                                                                                                   tATCAAATCATGACTTTTTTCCTGAAAAATCAGTAGATATTCATAGGCAAATAAAGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGAGCAAGCCGC‑ATATGTTGCTAAACGTCAGGAGTGCGCAAAATGATGCGACAATCACTTCAGGCTGTTTTACCTGAAATTTccg                                                                                                                                  >  1:350968/1‑249 (MQ=255)                                                                                                        aaTCATGACTTTTTTCCTGAAAAGTCAGTAGATATACATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCATGCCTC‑ATATGTTGCTAAACGTCAGGAGTGCGCAAAATGATGCGACAATCACTTCAGGCTGTTTTACCTGAAATTTCAGGCAAt                                                                                                                             <  1:33275/251‑1 (MQ=255)                                                                                                                    tttCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAgtgg                                                                                                                                                                                                                                    <  1:413829/137‑1 (MQ=255)                                                                                                                    tttCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAgtgg                                                                                                                                                                                                                                    >  2:413829/1‑137 (MQ=255)                                                                                                                          gAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGC‑ATATGTTGCTAAACGTCAGGAGTGCGCAAAATGATGCGACAATCACTTCAGGCTGTTTTACCTGAAATTTCAGGCAATAAAACGTCATCGCTGCGt                                                                                                           >  1:489293/1‑251 (MQ=255)                                                                                                                                   gTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGC‑ATATGTTGCTAAACGTCAGGAGTGCGCAAAATGATGCGACAATCACTTCAGGCTGTTTTACCTGAAATTTCAGGCAATAAAACGTCATCGCTGCGTAAATCGGt                                                                                                   >  1:322376/1‑250 (MQ=255)                                                                                                                                     aGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGC‑ATATGTTGCTAAACGTCAGGAGTGCGCAAAATGATGCGACAATCACTTCAGGCTGTTTTACCTGAAATTTCAGGCAATAAAACGTCATCGCTGCGTAAATCGGTAt                                                                                                 <  1:131966/250‑1 (MQ=255)                                                                                                                                      gATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGC‑ATATGTTGCTAAACGTCAGGAGTGCGCAAAATGATGCGACAATCACTTCAGGCTGTTTTACCTGAAATTTCAGGCAATAAAACGTCATCGCTGCGTAAATCGGTATGc                                                                                               >  1:345176/1‑251 (MQ=255)                                                                                                                                              tAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGC‑ATATGTTGCTAAACGTCAGGAGTGCGCAAAATGATGCGACAATCACTTCAGGCTGTTTTACCTGAAATTTCAGGCAATAAAACGTCATCGCTGCGTAAATCGGTATGCAGCGTtct                                                                                       >  2:233999/1‑251 (MQ=255)                                                                                                                                                      tAAGGTTTTATACTTTGCTGACAGGAGTCAGGCCTGTCGCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAGTGGCGCCCGGTGTTCAGGGCAAGCCGC‑ATATGTTGCTAAACGTCAGGAGTGCGCAAAATGATGCGACAATCACtt                                                                                                                                                           <  2:189662/175‑1 (MQ=255)                                                                                                                                                      tAAGGTTTTATACTTTGCTGACAGGAATCAGGCCTGTCTAAGACTGACTTGGATGTAATGAACAAAAGGGAATGGCTATGGGCAATGAGCATCAATACAGTGGTGCCCGGTGTTCGGGGCAAGCCGC‑ATATGTTGCTAAACGTCAGGAGTGAGCAAAATGATGCGACAATCACtt                                                                                                                                                           >  1:189662/1‑175 (MQ=255)                                                                                                                                                        aGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGC‑ATATGTTGCTAAACGTCAGGAGTGCGCAAAATGATGCGCCAATCACTTCAGGCTGTTTTACCTGAAATTTCAGGCAATAAAACGTCATCGCTGCGTAATTCGGTa                                                                                                  >  2:341087/1‑230 (MQ=255)                                                                                                                                                        aGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGC‑ATATGTTGCTAAACGTCAGGAGTGCGCAAAATGATGCGACAATCACTTCAGGCTGTTTTACCTGAAATTTCAGGCAATAAAACGTCATCGCTGCGTAAATCGGTa                                                                                                  <  1:341087/230‑1 (MQ=255)                                                                                                                                                            tttATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGC‑ATATGTTGCTAAACGTCAGGAGTGCGCAAAATGATGCGACAATCACTTCAGGCTGTTTTACCTGAAATTTCAGGCAATAAAACGTCATCGCTGCGTAAATCGGTATGCAGCGATCTCCTCACTCttttt                                                                          >  2:443832/1‑250 (MQ=255)                                                                                                                                                                      gCTGACAGGATTCAGGCCTGTCTCTGACTGACATGGATGTAATGAACAAAAGTGAATGTCTATGGGAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGTCGC‑ATATGTTGCTACACGTCAGGAGTGCGCAAAATGATGCCACATTCACTTCAGGCTGTTTTACCTGAAATTTCAGGCAATAAAGCGTCATCGCTTCGTAAATCGGTATGCAGCGATCTCCTCACTCTTTTTAATTCTCCTCa                                                               <  1:486287/251‑1 (MQ=255)                                                                                                                                                                       cTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGC‑ATATGTTGCTAAACGTCAGGAGTGCGCAAAATGATGCGACAATCACTTCAGGCTGTTTTACCTGAAATTTCAGGCAATAAAACGTCATCGCTGCGTAAATCGGTATGCAGCGATCTCCTCACTCTTTTTAATTCTCCTCAt                                                              >  1:162179/1‑251 (MQ=255)                                                                                                                                                                         gACAGGATTCAAGCCTGTCTCAGACGGACATGGATGTAATGAACAAAAGGGAAGGGCTATGGGAAATGAGCAGCAATACTGTGGTGCTCGGTGTTCAGGGCAAGCCGC‑ATATGTTTCTAAACGTCAGGAGTTCGCAAAATGATGCGACAATCACTGCAGGCTGTTTTACTTGAAATTTCAGGCAATAAAACGTCATCGCTGCGTAAATCGGTATGCAGCGATCTCCTCACTCTTTTTAAGCCTCCGCATTc                                                            <  2:463379/251‑1 (MQ=255)                                                                                                                                                                             ggATTCAGGCCTGTCACAGTCTGACATGGATGTAATGAACAAAAGGGAATGGCTATGTGAAACGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGC‑ATATGTTGCTAAACGTCAGGAGAGCGCAAAATGATGCGACAATCACTTCAGGATGTTTTACCTGAAATTTCAGGCAATCAAACGTCATAGCCGCGTAAATCGGTATGCAGCGATCTCCTCACTCTTTTTCATTCTCCTCATTCGGc                                                         >  2:65031/1‑250 (MQ=255)                                                                                                                                                                                 tCAGGCCTGTCGCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGC‑ATATGTTGCTAAACGTCAGGAGTGCGCAAAATGATGCGACAATCACTTCAGGCTGTTTTACCTGAAATTTCAGGCAATAAAACGTCATCGCTGCGTAAATCGGTATGCAGCGAGCTCCTCACTCTTTTTAATTCTCCTCATTCGGCATTa                                                     <  2:251356/250‑1 (MQ=255)                                                                                                                                                                                  cAGGCCTGTCTCAGACTGACATGTATGTAATGAACAAAAGGGAAGGGCTATGGGAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGC‑ATATGTTGCTAAACGTCAGGAGTGCGCAAAATGATGCGACAATCACTTCAGGCTGTTTTACCTGAAATTTCAGGCAATAAAACGTCATCGCTGCGTAAATCGGTATGCAGCGATCTCCTCACTCTTTTTAATTCTCCTCATTCGGCATTAc                                                    <  2:303546/250‑1 (MQ=255)                                                                                                                                                                                         gTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGC‑ATATGTTGCTAAACGTCAGGAGTGCGCAAAATGATGCGACAATCACTTCAGGCTGTTTTACCTGAAATTTCAGGCAATAAAACGTCATCGCTGCGTAAATCGGTATGCAGCGATCTCCTCACTCTTTTTAATTCTCCTCATTCGGCATTACCGTCAtt                                             <  1:125113/250‑1 (MQ=255)                                                                                                                                                                                         gTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGAAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGC‑ATATGTTGCTAAACGTCAGGAGTGCGCAAAATGATGCGACAATCACTTCAGGCTGTTTTACCTGAAATTTCAGGCAATAAAACGTCATCGCTGCGTAAATCGGTATGCAGCGATCTCCTCACTCTTTTTAATTCTCCTCATTCGGCATTACCGTCAt                                              <  2:440982/249‑1 (MQ=255)                                                                                                                                                                                         gTCTCAGACTGACATGGAGGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGC‑ATATGTTGCTAAACGTCAGGAGGGCGCAAAATGATGCGACAATCACTTCAGGCTGTTTTACCTGAAATTTCAGGCAATAAAACGTCATCGCTGCGTAAATCGGTATGCAGCGATCTCCTCACTCTTTTTAATTCTCCTCATTCGGCATTAACGTCAt                                              <  1:55704/249‑1 (MQ=255)                                                                                                                                                                                             cAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGC‑ATATGTTGCTAAACGTCAGGAGTGCGCAAAATGATGCGACAATCACTTCAGGCTGTTTTACCTGAAATTTCAGGCAATAAAACGTCATCGCTGCGTAAATCGGTATGCAGCGATCTCCTCACTCTTTTTAATTCTCCTCATTCGGCATTACCGTCATTACTTg                                        <  1:45787/251‑1 (MQ=255)                                                                                                                                                                                                 cTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGC‑ATATGTTGCTAAACGTCAGGAGTGCGCAAAATGATGCGACAATCACTTCAGGCTGTTTTACCTGAAATTTCAGGCAATAAAACGTCATCGCTGCGTAAATCGGTATGCAGCGATCTCCTCACTCTTTTTAATTCTCCTCATTCGGCATTACCGTCATTACTTGTTTc                                    >  2:60057/1‑251 (MQ=255)                                                                                                                                                                                                                  aaCAAAAGGGAATGGCTATGGGAAATGAGCAACAATACAGTGGTGCCCGGTGTGCAGGGCAAGCCGC‑ATATGTTGCTAAACGTCAGGAGTGCGCAAAATGATGCGACAATCACTTCAGGCTGTTTTACCTGAAATTTCAGGCAATAAAACGTCATCGCTGCGTAAATCGGTATGCAGCGATCTCCTCACTCTTTTTAATTCTCCTCATTCGGCATTACCGTCATTACTTGTTTCAGGCATGCCCGAGTGGc                   <  1:229164/251‑1 (MQ=255)                                                                                                                                                                                                                   aCAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGC‑ATATGTTGCTAAACGTCAGGAGTGCGCAAAATGATGCGACAATCACTTCAGGCTGTTTTACCTGAAATTTCAGGCAATAAAACGTCATCGCTGCGTAAATCGGTATGCAGCGATCTCCTCACTCTTTTTAATTCTCCTCATTCGGCATTACCGTCATTACTTGTTTCAGGCATGCCCGAGTGGCa                  <  1:454997/251‑1 (MQ=255)                                                                                                                                                                                                                               ggCTATGGGAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGC‑ATATGTTGCTAAACGTCAGGAGTGCGCAAAATGATGCGACAATCACTTCAGGCTGTTTTACCTGAAATTTCAGGCAATAAAACGTCATCGCTGCGTCAATCGGTATGCAGCGATCTCCTCACTCTTTTTAATTCTCCTCATTCGGCATTACCGTCATTACTTGTTTCAGGCATGCCCGAGTGGCAAGTGCATAAccc      >  2:62664/1‑251 (MQ=255)                                                                                                                                                                                                                                gCTATGGGAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGC‑ATATGTTGCTAAACGTCAGGAGTGCGCAAAATGATTCGACAATCACTTCTGGCTGTTTTACCTGAAATTTCAGGCAATAAAACGTCATCGCTGCGTAAATCGGTATGCAGCGATCTCCTCACTCTTTTTAATTCTCCTCATTCTGCATTACCGTCATTACTTGTTTCAGGCATGCCCGAGTGGCAAGTGCATAACCCg     <  1:129018/251‑1 (MQ=255)                                                                                                                                                                                                                                   aTGGGAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGC‑ATATGTTGCTAAACGTCAGGAGTGCGCAAAATGATGCGACAATCACTTCAGGCTGTTTTACCTGAAATTTCAGGCAATAAAACGTCATCGCTGCGTAAATCGGTATGCAGCGATCTCCTCACTCTTTTTAATTCTCCTCATTCGGCATTACCGTCATTACTTGTTTCAGGCATGCCCGAGTGGCAAGTGCATAACCCGAGc  >  2:136830/1‑251 (MQ=255)                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                        CCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGAAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGC‑ATATGTTGCTAAACGTCAGGAGTGCGCAAAATGATGCGACAATCACTTCAGGCTGTTTTACCTGAAATTTCAGGCAATAAAACGTCATCGCTGCGTAAATCGGTATGCAGCGATCTCCTCACTCTTTTTAATTCTCCTCATTCGGCATTACCGTCATTACTTGTTTCAGGCATGCCCGAGTGGCAAGTGCATAACCCGAGC  >  NC_000913/4556687‑4557163

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑