Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,514,287	G→C	L8V (CTT→GTT)	fecB ←	ferric citrate ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,514,287	0	G	C	100.0%	174.2 / NA	54	L8V (CTT→GTT)	fecB	ferric citrate ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (25/29);  total (25/29)

CGCGCGCGTTCCGACGGACTGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCAATACCGATCGGGATGACGTCCACGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCACCACAATCCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAAGAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACATCAGAACTTCAACGACCCCTGCATATACAGCGTGCGCGGCTGGCCTGCATAGATGCCTTTGTTGTTGTCGTCATAAGAGCGGATGAAGTAGTCCTGGTCGAAGATGTTTTTCACACCGAATGCCAGGTTCAGATCTGCCATCTGCGGGCCAAAGTCATACGCCACGCGTGCGCCCCAGAGC  >  NC_000913/4514045‑4514533                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                       cgcgcgcgGTCCGACGGACTGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCAATACCGATCGGGCTGACGTCCACGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCTCCACAATCCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAACAAAACGGa                                                                                                                                                                                                                                                <  1:266432/251‑1 (MQ=255)            cGACGGACTGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCAATACCGATCGGGCTGACGTCCACGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCACCACAATCCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCCAAGGCGTGGCTGATCACCCGCAGCAGGCCTGCAAAAACAAAACGGATaaaagacaac                                                                                                                                                                                                                                     >  2:117482/1‑244 (MQ=255)                ggACTGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCAATACCGATCGGGCTGACGTCCACGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCACCACAATCCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAACAAAACGGATAAATGCCAACATa                                                                                                                                                                                                                                  >  1:224097/1‑250 (MQ=255)                  gcggCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCAATACCGATCGGGCTGACGTCCACGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCACCACAATCCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAACAAAACGGATAAATGCCAACATAATc                                                                                                                                                                                                                               <  2:107494/248‑1 (MQ=255)                   cTGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCAATACCGATCGGGCTGACGTCCACGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCACCACAATCCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAACAAAACGGATAAATGCCAACATAATc                                                                                                                                                                                                                               >  1:332041/1‑250 (MQ=255)                     gCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCAATACCGATCGGGCTGACGTCCACGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCACCACAATCCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCTCCAGCAGCAGGCCTGCAAAAACAAAACGGATAAATGCCAACATAATcaca                                                                                                                                                                                                                            <  1:402014/251‑1 (MQ=255)                     gCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCAATACCGATCGGGCTGACGTCCACGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCACCACAATCCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAACAAAACGGATAAATGCCAACATAATca                                                                                                                                                                                                                              <  2:332041/249‑1 (MQ=255)                            tttCAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCAATACCGATCGGGCTGACGTCCACGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCACCACAATCCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAACAAAACGGATAAATGCCAACATAATCACATTcccgc                                                                                                                                                                                                                     >  1:94142/1‑248 (MQ=255)                              tCAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCAATACCGATCGGGCTGACGTCCACGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCACCACAATCCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAACAAAACGGATAAATGCCAACATAATCACATTCCAGCt                                                                                                                                                                                                                    <  1:157935/250‑1 (MQ=255)                                                    cAGGATGCGTTTTGCATCGTTATCGTCGGCAATACCGATCGGGCTGACGTCCACGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCACCACAATCCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGAGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAACAAAACGGATAAATGCCAACATAATCACATTCCAGATAAAAGCCCGGCAAGCCGGGCGtt                                                                                                                                                                                             >  1:411315/1‑251 (MQ=255)                                                           cGTTTTGCATCGTTATCGTCGGCAATACCGATCGGGCTGACGTCCACGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCACCACAATCCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAACAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAacaaa                                                                                                                                                                                       >  2:195386/1‑248 (MQ=255)                                                            gTTTTGCATCGTTATCGTCGGCAATACCGATCGGGCTGACGTCCACGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCACCACAATCCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCCGGCCTGCAAAAACAAAACGGATAAATGCCAACATAATCACATTCCCGCTAAAAGCCCAGCAAGCCGGGCGTTAACCCATc                                                                                                                                                                                     >  2:136227/1‑251 (MQ=255)                                                               ttGCATCGTTATCGTCGGCAATACCGATCGGGCTGACGTCCACGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCACCACAATCCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAACAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACATCAg                                                                                                                                                                                   <  1:6944/250‑1 (MQ=255)                                                                 gCATCGTTATCGTCGGCAATACCGATCGGGCTGACGTCCACGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCACCACAATCCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAACAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACATCAGAAc                                                                                                                                                                                >  1:330150/1‑251 (MQ=255)                                                                  caacGTTATCGGCGGCAATACCGAGCGGGGTTACGTCCACGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCACCACACTCCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCTCCAGCAGCAGGCCTGCAAAAACAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACATCAGAACt                                                                                                                                                                               <  2:399543/248‑1 (MQ=255)                                                                        tATCGTCGGCAATACCGATCGGGCTGACGTCCACGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCACCACAATCCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAACAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACATCAGAACTTCAAc                                                                                                                                                                          <  1:493884/250‑1 (MQ=255)                                                                            gtcgGCAATACCGATCGGGCTGACGTCCACGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCACCACAATCCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAACAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCAGGCAAGCCGGGCGTTAACACATCAGAACTTCAACAAccc                                                                                                                                                                     >  2:166524/1‑251 (MQ=255)                                                                                    tACCGATCGGGCTGACGTCCACGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCACCACAATCCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAACAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACATCAGAACTTCAACGACCCCTGCatat                                                                                                                                                             >  2:158673/1‑251 (MQ=255)                                                                                     aCCGATCGGGCTGACGGCCACGGCGGCCAGCGCATCTGCGAACGAGAGTTCCAGCACCACAATCCGTGGGGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGAGCACCAGCAGCAGGCCTGCAAAAACAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACATCAGAACTTCAACGACCCCTGCagat                                                                                                                                                             <  2:339581/250‑4 (MQ=255)                                                                                            gggctGACGTCCACGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCACCACAATCCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAACAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACATCAGAACTTCAACGACCCCTGCATATACAGCGTg                                                                                                                                                     <  1:480349/247‑1 (MQ=255)                                                                                              ggtGACGTCAACGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCCCCACAATCCGTTGTGGCGTTTTTTCGGGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAACAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACATCAGAACTTCAACGACCCCTGCATATACAGCGTgcg                                                                                                                                                   <  2:266539/249‑1 (MQ=255)                                                                                              gctGACGTCCACGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCACCACAATCCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAACAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACATCAGAACTTCAACGACCCCTGCATATACAGCGTgcg                                                                                                                                                   >  1:19440/3‑251 (MQ=255)                                                                                                 gACGTCCACGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCACCACAATCCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAACAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACATCAGAACTTCAACGACCCCTGCATATACAGCGTgcgc                                                                                                                                                  <  1:460872/249‑1 (MQ=255)                                                                                                 gACGTCCACGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCACCACAATCCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAACAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACATCAGAACTTCAACGACCCCTGCATATACAGCGTGCGCgg                                                                                                                                                >  1:106218/1‑251 (MQ=255)                                                                                                      ccACGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCACCACAATTCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAACAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACATCAGAACTTCAACGACCCCTGCATATACAGCGTGCGCGGCTgg                                                                                                                                            <  1:87261/250‑1 (MQ=255)                                                                                                            cggcgtgcgcATCGGCGAACGAGAGTTCGAGCACCACAATCCGTTGTGGCGTTTTTTCGAGTGTAAACGTTGCGTGTTCGGCCTGAACCGTGGCGGCAAAGGCGTGGCTGACCACCTGCAGCAGGCCTGCAAAAACAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACATCAGAACTTCCACGAACCCTGCATATACAGCGTGCGCGGCTGTCCTGCAt                                                                                                                                     <  1:223311/245‑1 (MQ=255)                                                                                                                cagcgcagcgGCGAACGAGAGTTCCAGCACCACAATCCGTTGTGGCGTTTTTTCGAGTGGAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAACAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACATCAGAACTTCAACGACCCCTGCATATACAGCGTGCGCGGCTGGCCTGCATAGAt                                                                                                                                 <  2:49326/251‑1 (MQ=255)                                                                                                                                         agcacccacagccgtgttgGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGGGGCTGATCACCAGCAGCAGGCCTGCAAAAACAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACAGCAGAACTTCAACGACCCCTGCATATACAGCGTGCGCGGCTGGCCTGCATAGAGGCCTGTGTGGTTGTCGTCATAagag                                                                                                        <  2:238473/234‑1 (MQ=255)                                                                                                                                           caccacAATCCGGTGTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAACAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACAGCAGAACTTCAACGACCCCTGCATATACAGCGTGCGCGGCTGGCCTGCATATATGCCTTTGTTGTTGTCGTCATAAGAGCg                                                                                                      <  1:406568/251‑1 (MQ=255)                                                                                                                                            accacAATCCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAACAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACATCAGAACTTCAACGACCCCTGCATATACAGCGTGCGCGGCTGGCCTGCATAGATGCCTTTGTTGTTGTCGTCATAATAGCgg                                                                                                     <  1:391862/251‑1 (MQ=255)                                                                                                                                                caATCCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCGGTTTCACCCTGAACCGTGGCGCCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAACAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGTGCGTTAACTCATCAGAACTTCAACGACCCCTGCATATACAGCGTGCGCGGCTGGCCTGCATAGATGCCTTTGTTGTTGTCGTCATAAGAGCGGATGa                                                                                                 <  2:268971/251‑1 (MQ=255)                                                                                                                                                   tCCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAACAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACATCAGAACTTCAACGACCCCTGCATATACAGCGTGCGCGGCTGGCCTGCATAGATGCCTTTGTTGTTGTCGTCATACGAGCGGATGa                                                                                                 >  1:61124/1‑248 (MQ=255)                                                                                                                                                      gTTGTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAACAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACATCAGAACTTCAACGACCCCTGCATATACAGCGTGCGCGGCTGGCCTGCATAGATGCCTTTGTTGTTGTCGTCATAAGAGCGGATGAagtagt                                                                                           <  1:373562/251‑1 (MQ=255)                                                                                                                                                         gctcttccgatctgAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAACAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACATCAGCACTTCAACGACCCCTGCATAAACAGCGTGCGCGGCTGGCCTGCATAGATGCCTTTGTTGTTGTCGTAATAAGAACGGATGAagtagtact                                                                                        >  2:493959/14‑248 (MQ=255)                                                                                                                                                          tgGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAACACAAAACGGATAAATGCCAACATAATCACCTTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACATCAGAACTTCAACGACCCCTGCATATACAGCGTGCGCGGCGGGCCTGCATAGATGCCTTTGTTGTTGTCGGCCTAAGAGCGGATGAAGTAGTACTg                                                                                       >  2:295493/1‑251 (MQ=255)                                                                                                                                                              gTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAACAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACATCAGAACTTCAACGACCCCTGCATATACAGCGTGCTCGGCTGGCCTGCATCGATGCCTTTGTTGTTGTCGTCCTAAGAGCGGTTGAAGTAGTCCTGGTCg                                                                                   >  2:255908/1‑251 (MQ=255)                                                                                                                                                              gTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAACAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACATCAGAACTTCAACGACCCCTGCATATACAGCGTGCGCGGCTGGCCTGCATAGATGCCTTTGTTGTTGTCGTCATAAGAGCGGATGAAGTAGTCCTGGTCg                                                                                   <  2:391895/251‑1 (MQ=255)                                                                                                                                                                tttttCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGCTCACCAGCAGCAGGCCTGCAAAAACAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTACCACATCAGAATTTCAACGACCCCTGCATATACAGCGTGCGCGGCTGGCCTGCATAGATGCCTttgttgttg                                                                                                                    >  2:156065/1‑216 (MQ=255)                                                                                                                                                                tttttCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAACAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACATCAGAACTTCAACGACCCCTGCATATACAGCGTGCGCGGCTGGCCTGCATAGATGCCTttgttgttg                                                                                                                    <  1:156065/216‑1 (MQ=255)                                                                                                                                                                     cGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAACAAAACGGATACATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGAGCGTTAACACATCAGCACTTCAACGACCCCTGCCTATACAGCGTGCGCGGCTGGCCTGCATAGATGCCTTTGATGTTGTCGTCATAAGAGCGGATGAAGTAGTCCTGGTAGCAGATGt                                                                            >  1:479671/1‑251 (MQ=255)                                                                                                                                                                     cGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAACAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACATCAGAACTTCAACGACCCCTGCATATACAGCGTGCGCGGCTGGCCTGCATAGATGCCTTTGTTGTTGTCGTCATAAGAGCGGATGAAGTAGTCCTGGTCGAAGATGt                                                                            <  1:158673/251‑1 (MQ=255)                                                                                                                                                                      gAGTGGAAACGTGCCGAGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGATGATCACCAGCAGCAGGCCTGAAAAAACAAAACGGATACATGCCAACATAATCACATTCCAGCTAAACGCCCGGCACGCCGGGCGTTAACAAATCAGAACTTCCACGCCCCCTGCATATACAGCGAGCGCGGCTGGCCTGCATAGATGCCTTTGTTGTTTTCGTAATTAGAGGGGCGGAAGTAGTCCTTGTCGAAGATGtt                                                                           >  2:182261/1‑251 (MQ=255)                                                                                                                                                                            aaaCGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAACAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACATCAGAACTTCAACGACCCCTGCATATACAGCGTGCGCGGCTGGCCTGCATAGATGCCTTTGTTGTTGTCGTCATAAGAGCGGATGAAGTAGTCCTGGTCGAAGATTTTTTTca                                                                      <  2:306727/250‑1 (MQ=255)                                                                                                                                                                             aaCGGGCCGTGTCCGTCCGGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAACAAAACGGATAAATGCCAACAGAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACATCAGAACTTCAACGACCCCTGCATATACAGCGTGCGCGGCTGGCCTGCATAGATGCCTGTGTTGTTGTCGTCAGAAGAGCGGATGAAGTAGTCCTGGTCGAAGATGTTTTTcaca                                                                    <  2:224097/251‑1 (MQ=255)                                                                                                                                                                                 tGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCCGGCCTGCAAAAACAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACATCAGAACTTCAACGACCCCTGCATATACAGCGTGCGCGGCTGGCCTGCATAGATGCCTTTGTTTTTGTCGTCATAAGAGCGGATGAAGGAGTCCTGGTCGAAGATGTTTTTCACACCg                                                                 >  2:24306/1‑250 (MQ=255)                                                                                                                                                                                                  aCCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAACAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGTGCGTTAACACATCAGAACTTCAACGACCCCTGCATATACAGCGTGCGCGGCTGGCCTGCATAGATGCCTTTGTTGTTGTCGTCATAAGAGCGGATGAAGTAGTCCTGGTCGAATATGTTTTTCACACCGAATGCCAGGTTCAGATCt                                               <  2:492974/251‑1 (MQ=255)                                                                                                                                                                                                      tGGCGGCACAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAACAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACATCAGAACTTCAACGACCCCTGCATATACAGCGTGCGCGGCTGGCCTGCATAGATGCCTTTGTTGTTGTCGTCATAAGAGCGGATGAAGTAGTCCTGGTCGAAGATGTTTTTCAAACCGAATGCCAGGTTCAGATCTGcc                                            >  2:336244/1‑250 (MQ=255)                                                                                                                                                                                                                        tGATCACCAGCAGCAGGCATGCAAAAACAAAACGGCTAAATGCCACCATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACATCAGAACTTCCACGACCCCTGCATATACAGCGTGCGCGGCTGGCCTGCATAGATGCCTTTGTTGTTGTCGTCATAAGAGCGGATGAAGTAGTCCTGGTCGAAGATGTTTTTCCCACCGAATGCCAGGTTCAGATCTGCCATCTGCGGGCCAAAGTCAt                         >  2:455855/1‑251 (MQ=255)                                                                                                                                                                                                                                  aagcagGCCGGCAAAAACAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACATCAGAACTTCAACGACCCCTGCATATACAGCGTGCGCGGCTGGCCTGCATAGATGCCGTTGTTGTTGTCGTCATAAGAGCGGATGAAGTAGTCCTGGTCGAAGATGTTTTTCACACCGAATGCCAGGGTCAGATCTGCCATCTGCGGGCCAAAGTCATACGCCAcgcg               <  2:94142/250‑1 (MQ=255)                                                                                                                                                                                                                                     cagGCCTGCAAAAACAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACATCAGAACTTCAACGACCCCTGCATATACAGCGTGCGCGGCTGGCCTGCATAGATGCCTTTGTTGTTGTCGTCATAAGAGCGGATGAAGTAGTCCTGGTCGAAGATGTTTTTCACACCGAATGCCAGGTTCAGATCTGCCATCTGCGGGCCAAAGTCATACGCCACGCGTgc            <  2:407113/251‑1 (MQ=255)                                                                                                                                                                                                                                          cTGCAAAAACAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACATCAGAACTTCAACGACCCCTGCATATACAGCGTGCGCGGCTGGCCTGCATAGATGCCTTTGTTGTTGTCGTCATAAGAGCGGATGAAGTAGTCCTGGTCGAAGATGTTTTTCACACCGAATGCCAGGTTCAGATCTGCCATCTGCGGGCCAAAGTCATACGCCACGCGGGCGcccc       >  2:100175/1‑251 (MQ=255)                                                                                                                                                                                                                                           tGCAAAAACAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACATCAGACCTTCAACGACCCCTGCATATACAGCGTGCGCGGCTGGCCTGCATAGATGCCTTTGTTGTTGTCGTCATAAGAGCGGATGAAGTAATCATGGTCGTAGATGTTTTTCACCCCGAATGCCAGGTTCAGATCGGCCATCTGCGGGCCACAGTCATACGCCACGCGTGCGcccc       >  2:235395/1‑250 (MQ=255)                                                                                                                                                                                                                                              aaaaaCAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACATCAGAACTTCAACGACCCCTGCATATACAGCGTGCGCGGCTGGCCTGCATAGATGCCTTTGTTGTTGTCGTCATAAGAGCGGATGAAGTAGTCCTGGTCGAAGATGTTTTTCACACCGAATGCCAGGTTCAGATCTGCCATCTGCGGGCCAAAGTCATACGCCACGCGTGCGCCCCa      <  1:307780/248‑1 (MQ=255)                                                                                                                                                                                                                                              aaaaaCAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCACGGCAAGCCGGGCGTTAACACATCAGAACTTCAACGACCCCTGCATATACAGCGTGCGCGGCTGGCCTGCATAGATGCCTTTGTTGTTGTCGTCATAAGAGCGGATGAAGTAGTCCTGGTCGAAGATGTTTTTCACACCGAATGCCAGGTTCAGATCTGCCATCTGCGGGCCAAAGTCATACGCCACGCGTGCGCCCCa      >  2:307780/1‑248 (MQ=255)                                                                                                                                                                                                                                               aaaacaaaacGGAGAAATGCCAACATAATCACATGCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACATCAGAACTTCAACGACCCCTGCATATACAGCGTGCGCGGCTGGCCTGCATAGATGCCTTTGTTGTTGTCGTCATAAGAGCGGATGAAGTAGTCCTGGTCTAAGATGTTTTTCACACCGAATGCCAGGTTCAGATCTGCCATCTGCGGGCCAAAGTCATACGCCACGCGTGCGCCCCAGAGc  <  1:239358/251‑1 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                       CGCGCGCGTTCCGACGGACTGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCAATACCGATCGGGATGACGTCCACGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCACCACAATCCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAAGAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACATCAGAACTTCAACGACCCCTGCATATACAGCGTGCGCGGCTGGCCTGCATAGATGCCTTTGTTGTTGTCGTCATAAGAGCGGATGAAGTAGTCCTGGTCGAAGATGTTTTTCACACCGAATGCCAGGTTCAGATCTGCCATCTGCGGGCCAAAGTCATACGCCACGCGTGCGCCCCAGAGC  >  NC_000913/4514045‑4514533

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑