| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,512,642 | T→C | V256V (GTA→GTG) | fecC ← | ferric citrate ABC transporter membrane subunit FecC |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,512,642 | 0 | T | C | 96.0% | 220.2 / ‑3.2 | 76 | V256V (GTA→GTG) | fecC | ferric citrate ABC transporter membrane subunit FecC |
| Reads supporting (aligned to +/- strand): ref base T (2/1); new base C (34/39); total (36/40) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.01e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01 | |||||||||||
AATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAAT‑CAGCGCCAGCACTGC‑GCCTGCGGGCAGAT‑CT‑CCGGGGAAGGCCAGCGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAAC‑GCCACC‑GGACCTGCTACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCAAACGTAGCCTCGTCAGGTTCACTCCCAGCGTATGGGCGGTGCTGTCGCTGAGGTTGAGCAGGTTCAGTTGATTCGCCAGCAGCAACACGACAGGGACTGCAGTGACCACCACCGGCAAGAGCTGCCAGACATCCTGCCAGCGGGCGTGGGACACTCCGCCTGCCAGCCAGTAAAAGATGCCGTAAGCATG > NC_000913/4512406‑4512881 | aaTTTTCATCCTCGCCTCCTCACAAGCCAGACAAGGCAAGGGCTGCCAAT‑CAGCGCCAGCACTGC‑GCCTGCGGGCAGCT‑CT‑CCGGGGAAGTCCAGTGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGCCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCGGCCCAGAAGCGCGCCCGATGTGGCACCAGCAGACCGCTCAAC‑‑CCACCCGGACCTGCCACGCtaacccacac > 2:78870/1‑242 (MQ=255) tttCATCCGCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAAT‑CAGCGCCAGCACTGC‑GCCTGCGGGCAGAT‑CT‑CCGGGGAAGGCCAGTGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAAC‑GCCACC‑GGACCTGCCACGCTGACgcacgcac < 1:83857/250‑1 (MQ=255) cccccccctcccCACACGCCAGAAAAAGCAAGGGC‑GGCAATACAGCGCCAGCCCTGC‑GCCTGCGGGCAGAT‑CT‑CCGGGGAAGGCCAGTGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAAC‑GCCACC‑GGACCTGCCACGCTGACGCACGCaccaacca < 2:406016/245‑1 (MQ=255) ccTCGCCTCCTCCCAAGCCAGACAAAGCAAGGGCTGCCAAT‑CAGCGCCAGCACTGC‑GCCGGCGGGCAGAT‑CT‑CCGGGAAAGGCCAGTGCGCGTGCGAGTACATCTGCCAGCAGCAGCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAAC‑GCCACC‑GGACCTGCCACGCTGACGCACGCACCAACCAg < 2:28929/251‑1 (MQ=255) cGCCTCCGCACAAGCCAGACAAAGCAAGGGCTGCCAAT‑CAGCGCCAGCACTGC‑GCCTGCGGGCAGAT‑CT‑CCGGGGAAGGCCAGTGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCAGGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAACC‑GCCACC‑GGGCCTGCCACGCTGACGCACGCACCAACCAGAag < 1:20986/251‑1 (MQ=255) cctcctCACAAGCCAGACAAAGCAAGGGCTGCCAAT‑CAGCGCCAGCACTGC‑GCCTGCGGGCAGAT‑CT‑CCGGGGAAGGCCAGTGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAAC‑GCCACC‑GGACCTGCCACGCTGACGCACGCACCAACCAGAagc > 1:127626/1‑250 (MQ=255) cctcACAAGCCAGACAAAGCAAGGGCTGCCAAT‑CAGCGCCAGCCCGGC‑GCCGGCGGGCAGAT‑CT‑CCGGGGAAGGCCAGTGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAAC‑GCCACC‑GGCCCTGCCACGCTGACGCACGCACCAACCAGAAgcagca < 2:483227/251‑1 (MQ=255) cccaacaAGCAAGAAAAAGCAAGGGCTGCCAAT‑CAGGGCCAGCACTGC‑GCCTGCGGGCAGAC‑CT‑CCGGGGAAGGCCAGTGCGCGGGCGAGTACATCTGCCAGCAGCATAAGCGTGGCCCCCAGCAGCATGCTCACTGGCCGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGAAGACCGATAAAC‑GCCACG‑GGACCAGCCACGCTGACGCACGCACCAACCAGAAgcagca < 2:224703/247‑1 (MQ=255) aaaGCAAGGGCTGCCAAT‑CAGCGCCAGCACTGC‑GCCTGCGGGCAGAT‑CT‑CCGGGGAAGGCCAGTGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGACGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGCTAAAC‑GCCCCC‑GGACCTGCCCCGCTGACGCACGCACCACCCAGAAGCCACACTAACCTATtgatc > 2:400198/1‑249 (MQ=255) aaaGCAAGGGCTGCCAAC‑AAGCGCCAGCACTGC‑GCCTGCGGGCAGAT‑CT‑CCGGGGAAGGCCAGTGCGCGGGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAAC‑GCCACC‑GGACCTGCCACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATtgat < 1:400198/249‑1 (MQ=255) aaGCAAGGGCTGCCAAT‑CAGCGCCAGCACTGC‑GCCTGCGGGCAGAT‑CT‑CCGGGGAAGGCCAGTGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAAC‑GCCACC‑GGACCTGCCACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGAc < 1:288981/251‑1 (MQ=255) aGGGCTGCCAAT‑CAGCGCCAGCACTGC‑GCCTGCGGGCAGAT‑CT‑CCGGGGAAGGCCAGTGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTTATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAAC‑GCCACC‑GGACCTGCCACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCAAAc < 1:167653/251‑1 (MQ=255) gccggCCAAT‑CACCGCCAGCAC‑GCAGCCTGCGGGC‑GATGCC‑CCGGGGAAGGCCAGTGCGCGTGCGAGCACATCTGCCACCAGCAGCGGCTTGGCCCCCAGCAGAATGCCCACTGGCAGTAGTTTGCGCTGTCCGAAGCCTGCCCAGAAGCGCGCCAGAGGTGGCACCAGCAGACCGATAAAC‑GCCACC‑GGAACTGCCACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCAAACGt < 2:305247/247‑1 (MQ=255) gccggCCAAC‑CACCGCCAGCACTGC‑GCCGGCGGGCAGA‑‑CTGCCGGGGAAGGCCAGTGCGCGGGCGAGTCCAGCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAAC‑GCCACC‑GGACCTGCCACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCAAACGt < 2:417939/247‑1 (MQ=255) ccAAT‑CAGCGCCAGCACTGC‑GCCTGCGGGCAGAT‑CT‑CCGGGGAAGGCCAGTGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAAC‑GCCACC‑GGACCTGCCACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCAAACGTAGccc > 2:417566/1‑250 (MQ=255) aaT‑CAGCGCCAGCACTGG‑CCCTGCGGGCAGAT‑CT‑CCGGGGAAGGCCAGTGCGCGTGCGGGTACATCTGCCAGCAGCAGCAGCGTGGCCCCCAGCAGCAGGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCTATAAAC‑GCCTCC‑GGACCTGCCACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCAAACGTAGCCTCg < 2:480266/251‑1 (MQ=255) aT‑CAGCGCCAGCACTGC‑GCCTGCGGGCAGAT‑CT‑CCGGGGAAGGCCAGTGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAAC‑GCCACC‑GGCCCTGCCACGCTGACGCCCGCCACAACCAGAAGCAGCACTAACATATTGATGACCAAACGTAGCCTCGt > 2:152084/1‑251 (MQ=255) cagcgccagcgcTGC‑GCCTGCGGGCAGAT‑CT‑CCGGGGAAGACCAGTGCGCGTGCGAGTACATCTGCCAACAGCATCAGATTGGCCCCCAGCAGCATGCTCACTGGCAGTCCGTTGCGCTGATCGAAGCCTGCCAAGAAGCGCGCAAGATGTGGCACCAGCCGACCGATAAAC‑GCCACC‑GGACCTCCCACGCTGACGCACGCACCAACCAGAAGCAGCACTAACACATTGCTGACCAGACGCACCCTCgcc > 2:352871/1‑248 (MQ=255) catcACTGC‑GCCTGCGGGCAGAT‑CT‑CCGGGGAAGGCCAGTGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCACAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAAC‑GCCACC‑GTACCTGCCACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCAAACGTAGCCTCGTCAGGTTCa < 1:170926/248‑1 (MQ=255) cACTGC‑GCCTGCGGGCAGAT‑CT‑CCGGGGAAGGCCAGTGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAAC‑GCCACC‑GGACCTGCCACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCAAACGTAGCCTCGTCAGGTTCACTc > 2:91177/1‑251 (MQ=255) aCTGC‑GCCTGCGGGCAGAT‑CT‑CCGGGGAAGGCCAGTGCGCGTGCGAGTACCTCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAAC‑GCCACC‑GGACCTGCCACGCTGACGCCCGCACCAACCAGAAGCAGCACTAACATATTGATGACCAAACGTAGCCTCGTCAGGTTCACTcc > 2:60617/1‑251 (MQ=255) gc‑gcCTGCGGGCAGAT‑CT‑CCGGGGAAGGCCAGTGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAAC‑GCCACC‑GGACCTGCCACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCAAACGTAGCCTCGTCAGGTTCACTccc < 1:349777/249‑1 (MQ=255) gCGGGCAGAC‑CT‑CCGGGGAAGGCCAGTGCGCGTGCGAGGACCTCTGCCCGAAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCTGTCCGTTGCGCTGATCGAAGCCTGCCCAGAGGCGCGCCAGATGTGGCTCCAGCAGACCGATAAAC‑GCCACC‑GGACCTGCCACGCTGACGCACGCACCAACCAGAAGCAGCCCTAACATATTGATGACCAAACGTAGCCTCGTCAGGTTCACTCCCAGCGTa < 2:275679/249‑1 (MQ=255) ggtgccagtcct‑cCGGGGCAGGCCCGTGCGCGTGCGAGTACATCTGCCAACAGCCTCAGCGTGCCCCCCAGCAGCTTGCTCACTGGCAGTCCGTTGCGCTGACCGAAGCCTGCCCAGAAGCGCGCCAGAGGTGGCACCAGCAGACCGATAACC‑GCCACC‑GGACCTGCCACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCAAACGTAGCCTCGTCAGGTTCACTCCCAGCGTATg < 2:386581/241‑1 (MQ=255) ccGGGGAGGGCCCGTGCGCGTGCGAGTACATCCGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCACAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAAC‑GCCACC‑GGACCTGCCACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCAAACGTAGCCTCGTCAGGTTCACTCCCAGCGTATGGGCGGTGCTg < 2:268333/249‑1 (MQ=255) ccGGGGAAGGCCAGTGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAAC‑GCCACC‑GGACCTGCCACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCAAACGTAGCCTCGTCAGGTTCACTCCCAGCGTATGGGCGGTGCTGTc < 1:505107/251‑1 (MQ=255) ccGGGGAAGGCCAGTGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAAC‑GCCACC‑GGACCTGCCACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCAAACGTAGCCTCGTCAGGTTCACTCCCAGCGTATGGGCGGTGCTGTc > 1:465644/1‑251 (MQ=255) cGGGGAAGGCCAGTGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCCTGCTCCCTGGCAGTACGTTGCACTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAAC‑TCCACC‑GGCCCTGCCCCGCTGACGCCCGCACCACCCAGAAGCAGCACTAACATATTGAGGACACAACGAAACCTCGTCAGGTTCCCTCCCCGCGTATGGGAGGTgaggtcc > 2:164414/1‑245 (MQ=255) ggggAAGGCCAGTGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAAC‑GCCACC‑GGACCTGCCACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCAAACGTAGCCTCGTCAGGTTCACTCCCAGCGTATGGGCGGTGCTGTCGc > 1:366981/1‑251 (MQ=255) agtgcgcgTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAAC‑GCCACC‑GGACCTGCCACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCAAACGTAGCCTCGTCAGGTTCACTCCCAGCGTATGGGCGGTGCTGTCGCTGAGGTTg > 1:319440/4‑249 (MQ=255) agtgcgcgTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAAC‑GCCACC‑GGACCTGCCACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCAAACGTAGCCTCGTCAGGTTCACTCCCAGCGTATGGGCGGTGCTGTCGCTGAGGTTg > 1:252803/4‑249 (MQ=255) tgcgcgTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAAC‑GCCACC‑GGACCTGCCACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCAAACGGAGCCTCGTCAGGTTCACTCCCAGCGTATGGGCGGTGCTGTCGCTGAGGTTGAGc > 1:370654/2‑250 (MQ=255) tgcgcgTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGAACCAGCAGACCGATAAAC‑GCCACC‑GGACCTGCCACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCAAACGTAGCCTCGTCAGGCTCACTCCCAGCGTATGGGCGGTGCTGTCGCTGGGGTTGAGcg > 2:311214/2‑250 (MQ=255) ggcgcgTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATTCTCACTGGCAGTACGTTGCGCTGATCGAAGCCGGCCCATAAGCGCGCCAGATGTGGCGCCAGCAGACCGATAAAC‑GCCCCC‑GGACCTGCCACGCTGACGCACGCACCAACCAGAAGCATCACTAACATATTGATGACCAAACGTAGCCTCGTCAGGTTCACTCCCAGCGTATGGGCGGTGCTGTCGCTGAGGTTGAGCa < 1:493240/250‑1 (MQ=255) cGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAAC‑GCCACC‑GGACCTGCCACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCAAACGTAGCCTCGTCAGGTTCACTCCCAGCGTATGGGCGGTGCTGTCGCTGCGGTTGAGCAGGTTCAGt > 1:110834/1‑251 (MQ=255) gTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAAC‑GCCACC‑GGACCTGCCACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCAAACGTAGCCTCGTCAGGTTCACTCCCAGCGTATGGGCGGTGCTGTCGCTGAGGTTGAGCAGGTTCAGTTGa < 1:123359/251‑1 (MQ=255) aCATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAAC‑GCCACC‑GGACCTGCCACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCAAACGTAGCCTCGTCAGGTTCACTCCCAGCGTATGGGCGGTGCTGTCGCTGAGGTTGAGCAGGTTCAGTTGAtt < 1:60617/251‑1 (MQ=255) ccgacgtccgcagcggcgggccccccTGCAGAATGCTCTCGGGCAGTCCGTGGCTCTGATGAAACCCTCCCCAGAGGCGCGCTATATGTGGCACCAGAAGACAGATAAAC‑GCCACC‑GTACCTGCCACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCAAACGTAGCCTCGTCAGGTTCACTCCCAGCGTATGGGCGGTGCTGTCGCTGAGGTTGAGCAGGTTCAGTTGATTCGcc < 2:5984/230‑1 (MQ=255) gCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAAC‑GCCACC‑GGACCTGCCACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCAAACGTAGCCTCGTCAGGTTCACTCCCAGCGTATGGGCGGTGCTGTCGCTGAGGTTGAGCAGGTTCAGTTGATTCGcc > 1:498380/1‑249 (MQ=255) cagcagCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAAC‑GCCACC‑GGACCTGCTACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGCTGACCAAACGTAGCCTCGTCCGGTTCACCCCCAGCGTATGGGCGGTGCTGTCGCTTGGGTTGAGCAGGTTCAGTTGATTCGCcagc > 2:18250/1‑250 (MQ=255) agcagcATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAAC‑GCCACC‑GGACCTGCCACGATGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCAAACGTAGCCTCGTCAGGTTCACTCCCAGCGTATGGGCGGTGCTGTCGCTGAGGTTGAGCAGGGTCAGTTGAATCGCcagc > 2:243341/1‑249 (MQ=255) caTCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAAC‑GCCACC‑GGACCTGCCACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCAAACGTAGCCTCGTCAGGTTCACTCCCAGCGTATGGGCGGTGCTGTCGCTGAGGTTGAGCAGGTTCAGTTGATTCGCCAGCAGCAac > 1:300725/1‑250 (MQ=255) tCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAAC‑GCCACC‑GGACCTGCCACGCTGACGCACGCACCAAACAGAAGCAGCACTAACATATTGATGACCAAACGTAGCCTCGTCAGGTTCACTCCCAGCGTATGGGCGGTGCTGTCGCTGAGGTTGAGCAGGTTCAGTTGATTCGCCAGCAGCAACACg > 1:65956/1‑251 (MQ=255) cAGCGTGGCCCCCAGCAGCAGGCGCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAAC‑GCCACC‑GGACCTGCCACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCAAACGTAGCCTCGTCAGGTTCACTCCCAGCGTATGGGCGGTGCTGTCGCTGAGGTTGAGCAGGTTCAGTTGATTCGCCAGCAGCAACACGa < 2:319440/251‑1 (MQ=255) tGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAAC‑GCCACC‑GGCCCTGCCACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCAAACGTAGCCTCGTCAGGTTCACTCCCAGCGTATGGGCGGAGCGGTCGCTGAGGTTGGGCAGGTTCAGATGCTTCGCCAGCAGCAACACGCCAggg > 2:335122/1‑251 (MQ=255) ggCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAAC‑GCCACC‑GGACCTGCCACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCAAACGTAGCCTCGTCAGGTTCACTCCCAGCGTATGGGCGGTGCTGTCGCTGAGGTTGAGCAGGTTCAGTTGATTCGCCAGCAGCAACACGACAgggg > 2:235123/1‑250 (MQ=255) gCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGCTAAAC‑GCCACC‑GGACCTGCCACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCAAACGTAGCCTCGTCAGGTTCAATCCCAGCGTAAGGGGGGTGCTGTCGTTGCGGTTGAGCAGGTTCAGTTGCTTCGCCAGCAGCAacccccccgggcc > 2:472665/1‑240 (MQ=255) gCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAAC‑GCCACC‑GGACCTGCCACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCAAACGTAGCCTCGTCAGGTTCACTCCCAGCGTATGGGCGGTGCTGTCGCTGAGGTTGAGCAGGTTCAGTTGATTCGCCAGCAGCAACACGACAGGGAc < 1:113622/251‑1 (MQ=255) cccccAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAAC‑GCCACC‑GGACCTGCCACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCAAACGTAGCCTCGTCAGGTTCACTCCCAACGTATGGGCGGTGCTGTCGCTGAGGCTGTGCAGGGTCAGTTGATTCGCCAGCAGCAACACGACAggggcg > 1:138489/1‑248 (MQ=255) ccccAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGCTAAAC‑GCCACC‑GGACCTGCTACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCAAACGTAGCCTCGTCAGGTTCACTCCCAGCGTATGGGCGGTGCTGTCGCTGCGGTTGAGCAGGGTCCGTTGATTCGCCAGCCGCAACCCGACAGGGAcgg > 2:186218/1‑249 (MQ=255) agcagcATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAAC‑GCCACC‑GGACCTGCCACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCAAACGTAGCCTCGTCAGGTTCACTCCCAGCGTATGGGCGGTGCTGTCGCTGAGGTTGAGCAGGTTCAGTTGATTCGCCAGCAGCAACACGACAGGGACTGCAGt < 1:417566/251‑1 (MQ=255) cagcaTGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAAC‑GCCACC‑GGACCTGCCACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCAAACGTAGCCTCGTCAGGTTCACTCCCAGCGTATGGGCGGTGCTGTCGCTGAGGTTGAGCAGGTTCAGTTGATTCGCCAGCAGCAACACGACAGGGGCTGCAGTg > 1:455337/1‑250 (MQ=255) gcaTGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAAC‑GCCACC‑GGACCTGCCACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCAAACGTAGCCTCGTCAGGTTCACTCCCAGCGTATGGGCGGTGCTGTCGCTGAGGTTGAGCAGGTTCAGTTGATTCGCCAGCAGCAACACGACAGGGACTGCAGTGacc < 1:152084/251‑1 (MQ=255) cacgggcagtactgtgcgcggagagAAGCCTGGCCGGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAAC‑GCCACC‑GGCCCTGCCACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCAAACGTAGCCTCGTCAGGTTCACTCCCAGCGTATGGGCGGTGCTGTCGCTGAGGTTGAGCAGGTTCAGTTGATTCGCCAGCAGCAACACGACAGGGACTGCAGTGaccaccac < 2:310013/225‑1 (MQ=255) tGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAACC‑GCCACC‑GGACCTGCCACGCTGACGCACGCACCAACCAGAagca > 1:221519/1‑113 (MQ=255) tGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCAAGATGTGGCACCAGCAGACCGATAAAC‑GCCCCC‑GGACCTGCCACGCTGACGCACGCACCAACCAGAagca < 2:221519/113‑1 (MQ=255) cAGTACGTTGCGCTGATAGAAGCCTGCCAAGAAGCGCGCCAGATGTGGCCCCAGCAGACCGCTAACC‑GCCACC‑GGACCTGCCACGCTGACGCACGCACCAACAAGACACAGCACTAACATATTGATGACCAAACGTAGCCTCGTAAGGTTCACTCCCAGCGTATGGGCGGGGCAGTCGCTGAGGATGAGCAGGTTCAGTTGATTCGCACGCAGCAACACGACAGGGACTGCAGttgccacaacaggcaaa > 2:244353/1‑234 (MQ=255) tGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAAC‑GCCACC‑GGACCTGCCACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCAAACGTAGCCTCGTCAGGTTCACTCCCAGCGTATGGGCGGTGCTGTCGCTGAGGTTGAGCAGGTTCAGTTGATTCGCCAGCAGCAACACGACAGGGACTGCAGTGACCACCACCGGCAAGAGCTGCCAGACATc > 1:100059/1‑251 (MQ=255) gATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAAC‑GCCACC‑GGACCTGCCACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCAAACGTAGCCGCGTCAGGTTCACTCCCAGCGTATGGGCGGTGCTGTCGCTGAGGTTGAGCAGGTTCAGTTGATTCGCCAGCAGCAACACGACAGGGACTGCAGTGACCACCACCGGCAAGAGCTGCCAGACAt < 1:260891/249‑1 (MQ=255) ccGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAAC‑GCCACC‑GGACCTGCCACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCAAACGTAGCCTCGTCAGGTTCACTCCCAGCGTATGGGCGGTGCTGTCGCTGAGGTTGAGCAGGTTCAGTTGATTCGCCAGCAGCAACACGACAGGGACTGCAGTGACCACCACCGGCAAGAGCTGCCAGACATCCTg < 1:189931/250‑1 (MQ=255) aGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAAC‑GCCACC‑GGACCTGCCACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCAAACGTAGCCTCGTCAGGTTCACTCCCAGCGTATGGGCGGTGCTGTCGCTGAGGTTGAGCAGGTTCAGTTGATTCGCCAGCAGCAACACGACAGGGACTGCAGTGACCACCACCGGCAAGAGCTGCCAGACATCCTGcc > 1:141990/1‑249 (MQ=255) gccgccccagagacgcgccgaaggtgGCAACAGCAGACCTATAACC‑GCCACC‑GCACCAGCCACGCTGACGCACGCACCAACCAGAAGCAGCCCTAACATATTGAGGACCAAACGTAGCCTCGTTAGGTTCACTCCCAGCTTATGGGCGGTGCTGTCGCTGAGGTTGAGCAGGTTCAGTTGATTCGCCAGCAGCAACACGACAGGGACTGCAGTGACCACCACCGGCAAGAGCTGCCAGACATCCTGCCAGc < 2:344933/228‑1 (MQ=255) tGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAAC‑GCCACC‑GGACCTGCCACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCAAACGTAGCCTCGTCAGGTTCACTCCCAGCGTATGGGCGGTGCTGTCGCTGAGGTTGAGCAGGTTCAGTTGATTCGCCAGCAGCAACACGACAGGGACTGCAGTGACCACCACCGGCAAGAGCTGCCAGACATCCTGCCAGCggg > 1:412878/1‑251 (MQ=255) cAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAAC‑GCCACC‑GGACCTGCCACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCAAACGTAGCCTCGTCAGGTTCACTCCCAGCGTATGGGCGGTGCTGTCGCTGAGGTTGAGCAGGTTCAGTTGATTCGCCAGCAGCAACACGACAGGGACTGCAGTGACCACCACCGGCAAGAGCTGCCAGACATCCTGCCAGCGGGCGTg < 1:235123/251‑1 (MQ=255) cgcCAGATGTGGCCCCAGCAGACCGATAAAC‑GCCACC‑GGACCTGCCACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCAAACGTAGCCTCGTCAGGTTCACTCCCAGCGTATGGGCGGTGCTGTCGCTGAGGTTGAGCAGGTTCAGTTGATTCGCCAGCAGCAACACGACAGGGACTGCAGTGACCACCACCGGCAAGAGCTGCCAGACATCCTGCCAGCGGGCGTGGGACACTc < 1:360095/251‑1 (MQ=255) cAGATGTGGCTCCAGCAGACCGATAAAC‑GCCACC‑GGACCGGCCACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCAAACGTAGCCTCGTCAGGTTCACTCCCAGCGTATGGGCGGTGCTGTCGCTGAGGTTGAGCAGGTTCAGTTGATTCGCCAGCAGCAACACGACAGGGACTGCAGTGACCACCACCGGCAAGAGCTGCCAGACATCCTGCCAGCGGGCGTGGGACACTCCGc < 1:52706/251‑1 (MQ=255) ggcacaaccagACCGA‑AAACAGCCCCC‑GGACCTGCCACGCGGACGCCCGCACCAACCAGAAGCAGCATGAACATATTGAGGACCAAACGTAGCCGCGTCAGGTTCACTCCCAGCGTATGGGCGGTGCTGTCGCTGAGGTTGAGCAGGTTCAGTTGATTCGCCAGCAGCAACACGACAGGGACTGCAGTGACCACCACCGGCAAGAGCTGCCAGACATCCGGCCAGCGGGCGTGGGACACTCCGCCTgccag < 2:377677/243‑1 (MQ=255) cacgcgaaCCGATAACC‑CCCCCC‑GGCCCTCCCACGCCGACGCCCGCCCCAACCAGAAGCAGCACTAACATATGGACGCCCAACCGGAGCCTCGTCAGGTTCACTCCCAGCGTATGGGCGGTGCTGTCGCTGAGGTTGAGCAGGTTCAGTTGATTCGCCAGCAGCAACACGACAGGGACTGCAGTGACCACCACCGGCAAGAGCTGCCAGACATCCTGCCAGCGGGCGTGGGACACTCCGCCTGccagccag < 2:397692/244‑1 (MQ=255) cagACCGATAAAC‑GCCACC‑GGACCTGCCACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCAAACGTAGCCTCGTCAGGTTCACTCCCAGCGTATGGGCGGTGCTGTCGCTGAGGTTGAGCAGGTTCAGTTGATTCGCCAGCAGCAACACGACAGGGACTGCAGTGACCACCACCGGCAAGAGCTGCCAGACATCCTGCCAGCGGGCGGGGGACACTCCGCCTGCCCGCCAGTaa > 2:102921/1‑250 (MQ=255) agACCGATAAAC‑GCCACC‑GGACCTGCTACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCAAACGTAGCCTCGTCAGGTTCACTCCCAGCGTATGGGCGGTGCTGTCGCTGAGGTTGAGCAGGTTCAGTTGATTCGCCAGCAGCAACACGACAGGGACTGCAGTGACCACCACCGGCAAGAGCTGCCAGACATCCTGCCAGCGGGCGTGGGACACTCCGCCTGCCAGCCAGTaaaa < 1:18250/251‑1 (MQ=255) aaaC‑GCCACC‑GGACCTGCCACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCAAACGTAGCCTCGTCAGGTTCACTCCCAGCGTATGGGCGGTGCTGTCGCTGAGGTTGAGCAGGTTCAGTTGATTCGCCAGCAGCAACACGACAGGGACTGCAGTGACCACCACCGGCAAGAGCTGCCAGACATCCTGCCAGCGGGCGTGGGACACTCCGCCTGCCAGCCAGTAAAAGATGCCGt > 1:473332/1‑251 (MQ=255) cACC‑GGACCTGCCACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCAAACGTAGCCTCGTCAGGTTCACTCCCAGCGTATGGGCGGTGCTGTCGCTGAGGTTGAGCAGGTTCAGTTGATTCGCCAGCAGCAACACGACAGGGACTGCAGTGACCACCACCGGCAAGAGCTGCCAGACATCCTGCCAGCGGGCGTGGGACACTCCGCCTGCCAGCCAGTAAAAGATGCCGTAAGCAt < 1:91177/251‑1 (MQ=255) aCC‑GGACCTGCCACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCAAACGTCGCCTCGTCAGGTTCACTCCCAGCGTATGGGCGGTGCTGTCGCTGAgg > 2:454072/1‑114 (MQ=255) aCC‑GGACCTGCCACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCAAACGTAGCCTCGTCAGGTTCACTCCCAGCGTATGGGCGGTGCTGTCGCTGAgg < 1:454072/114‑1 (MQ=255) cccgaaCCTGCCACGCTGACGCACGCCCCAACCAGAAGCAGCACTAACATATTGATGACCAAACGTAGCCTCGTCAGGTTCACTCCCAGCGTATGGGCGGTGCTGTCGCTGAGGTTGAGCAGGTTCAGTTGATTCGCCAGCAGCAACACGACAGGGACTGCAGTGACCACCACCGGCAAGAGCTGCCAGACATCCTGCCAGCGGGCGTGGGACACTCCGCCTGCCAGCCAGTAAAAGATGCCGTAAGCATg < 1:491137/246‑1 (MQ=255) cc‑GGACCTGCCACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCAAACGTAGCCTCGTCAGGTTCACTCCCAGCGTATGGGCGGTGCTGTCGCTGAGGTTGAGCAGGGTCAGTTGATTCGCCAGCAGCACCACGACCGGGACTGCAGTGACCACCACAGGCAAGAGCTGCCCGACATCCTGCCAGCCGGCGATGGACACGCCGCCTGCCAGACAGCAAAAGAtaacgaacacatg > 2:104214/1‑238 (MQ=255) | AATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAAT‑CAGCGCCAGCACTGC‑GCCTGCGGGCAGAT‑CT‑CCGGGGAAGGCCAGCGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAAC‑GCCACC‑GGACCTGCTACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCAAACGTAGCCTCGTCAGGTTCACTCCCAGCGTATGGGCGGTGCTGTCGCTGAGGTTGAGCAGGTTCAGTTGATTCGCCAGCAGCAACACGACAGGGACTGCAGTGACCACCACCGGCAAGAGCTGCCAGACATCCTGCCAGCGGGCGTGGGACACTCCGCCTGCCAGCCAGTAAAAGATGCCGTAAGCATG > NC_000913/4512406‑4512881 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |