Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,507,424	G→C	pseudogene (228/255 nt)	insO →	IS911B regulator fragment

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,507,424	0	G	C	100.0%	167.1 / NA	53	pseudogene (228/255 nt)	insO	IS911B regulator fragment
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (24/29);  total (24/29)

GCTCCAATGAAAAAAAGAAATTTCAGCGCAGAGTTTAAACGCGAATCCGCTCAACTGGTTGTTGACCAGAAATACACGGTGGCAGATGCCGCGAAAGCTATGGATGTTGGCCTTTCCACAATGACAAGATGGGTCAAACAACTGCGTGATGAGCGTCAGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGGATTGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAGATTCAATCTGTCAATGCAACACCCCTTTCAATTATCTCTTTCGGTGTTTTGAACTTCAGTGTCTTTCTCGGTCTGTTGTTTAGCTGAGCAGCAACCAGATCTAGTTCATGTTGAGTATATTGGGCAAGACATGTCTTTTTAGGAAAGTACTGCCGAATTAGCCCATTTGTGTTCTCATTTGTTCCCCGCTGCCAAGGACTCTGAGGAT  >  NC_000913/4507191‑4507671                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                        gCTCCAATGAAAAAAAGAAATTTCAGCGCAGAGTTGAAACGCGAATCCGCTCAACTGGTTGTTGACCAGAAATACACGGTGGCAGATGCCGCGAAAGCTATGTATGTTGGCCTTTCCACAATGACAAGATGGGTCAAACAACTGCGTGATGAGCGTCAGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGCATTGAAATGGAGAATGa                                                                                                                                                                                                                                        <  1:261797/251‑1 (MQ=255)   tCCAATGAAAAAAAGAAATTTCAGCGCAGAGTTTAAACGCGAATCCGCTCAACTGGTTGTTGACCAGAAATACACGGTGGCAGATGCCGCGAAAGCTATGGATGTTGGCCTTTCCACAATGACAAGATGGGTCAAACAACTGCGTGATGAGCGTCAGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGCATTGAAATGGAGAATGaa                                                                                                                                                                                                                                       >  2:449792/1‑250 (MQ=255)          aaaaaaaGAAATTTCAGCGCAGAGTTTAAACGCGAAGCCGCTCAACTGGTTGTTGACCAGAAATACACGGTGGCAGATGCCGCGAAAGCTATGGATGTTGGCCTTTCCACAATGACAAGATGGGTCAAACAACTGCGTGATGAGCGTCAGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGCATTGAAATGGAGAATGAAATATTaaa                                                                                                                                                                                                                               <  2:163292/251‑1 (MQ=255)            aaaaaGAAATTTTAGCGCAGAGTTTAAACGCGAATCCGCTCAACTGGTTGTTGACCAGAAATACACGGTGGCAGATGCCGCTAAAGCTATGGATGTTGGCCTTTCCACAATGACAAGATGGGTCAAACAACTGCGTGATGAGCGTCAGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGCATTGAAATGGAGAATGAAATATTaaaaa                                                                                                                                                                                                                             <  1:431383/251‑1 (MQ=255)             aaaaGAAATTTCAGCGCAGAGTTTAAACGCGAATAAGCTCAACTGGTAGTTGACCAGAAATACACGGTGGCAGATGCCGCGACAGATATGGATGTTGGCCTTTCCAAAATGACAAGATGGGGCAAACAACTGCGTGATGAGCGTCAGGGCAAACCACCACAAACCTCACCGCAAACACCAGAACAAATCGAAAAAAGTAAGCTGAGGAAAAAGCCACAACGCATTAAAATGGAGAATGAAATATTaaaacg                                                                                                                                                                                                                            >  2:463123/1‑249 (MQ=21)               aaGAAATTGCAGTGCATCGTTTAAACGCGAATCCGCTCAACTGGTTGTTGACCAGAAATACACTGTGGCAGATGCCGCGAAAGCTATGGATGTTGGCCTTTCCACAATGACAAGATGGGTCAAACAACTGCTTGATGAGCGTCAGGGCAAAACACCAAAAGCCTATCCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGCATTGAAATGGAGAATGAAATAGTAAAAAGGc                                                                                                                                                                                                                          <  2:111149/251‑1 (MQ=255)                       tCAGGGCAGAGTTTAAACGCGAATCCGCTCAACTGGGTGTTGACCAGAAATACACGGTGGCAGATGCCGCGAAAGCTATGGATGTTGGCCTTTCCACAATGACAAGATGGGTCAAACAACTACGTGATGAGCGTCAGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGCATTGAAATGGAGAATGAAATATTAAAAAGGCTaccgcg                                                                                                                                                                                                                   >  2:153088‑M2/1‑244 (MQ=255)                        cAGCGCAGAGTTTAAGCGCGCATCCACTCAAATGGTTGTTTCCCAGACATACAAGATGGCAGATGCCGCGAAAGCTATGGCTGTTGGCCTTTCCACAATGACAAGATGGGTCAAACAACTGCGTGATGAGCGTCAGGGAAAAACACCAAACGCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACCACGCATTGAACTGGAGAGTGAAATATTAAAAGGGCAaccgcgct                                                                                                                                                                                                                 <  2:248950‑M2/251‑9 (MQ=255)                          gcgcAGAGTTTAAACGCGAATCCGCTCAACTGGTTGTTGACCAGAAATACACGGTGGCAGATGCCTCGAAAGCTATGGATGTTGGCCTTTCCACAATGACAAGATGGGTCAAACAACTGCGTGATGAGCGTCAGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGCATTGAAATGGAGAATGAAATATTAAAAAGGCTaccgcgctct                                                                                                                                                                                                               >  2:418017‑M2/1‑241 (MQ=255)                                   ttAAACGCGAATCCGCTCAACTGGTTGTTGACCAGAAATACACGGTGGCAGATGCCGCGAAAGCTATGGATGTTGGCCTTTCCACAATGACAAGATGGGTCAAACAACTGCGTGATGAGCGTCAGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAAGCGAAATACGTAAGCTGAGGAAAAAGCTACAACGCATTGAAATGGAGAATGAAATATTAAAAAGGCTaccgcgcccttgatgtc                                                                                                                                                                                                        <  1:95822‑M2/249‑18 (MQ=255)                                         gcgAATCCGCTCAACTGGTTGTTGACCATAAATACACGGTGGCAGATGCCGCGAAAGCTATGGATGTTGGCCTTTCCACAATGACAAGATGGGTCAAACAACTGCGTGATGAGCGTCAGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGCATTGAAATGGAGAATGAAATATTAAAAAGGCTaccgcgctcttgatgtcagactccc                                                                                                                                                                                                >  2:97948‑M2/1‑226 (MQ=255)                                            aaTCCGCTCAACTGGTTGTTGACCAGAAATACACGGTGGCAGATGCCGCGAAAGCTATGGATGTTGGCCTTTCCACAATGACAAGATGGGTCAAACAACTGCGTGATGAGCGTCAGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGCATTGAAATGGAGAATGAAATATTAAAAAGGCTaccgcgctcttgatgtcagactccctg                                                                                                                                                                                              <  1:305347‑M2/250‑28 (MQ=255)                                             aTCCGCTCAACTGGTTGTTGACCAGAAATACACGGTGGCAGATGCCGCGAAAGCTATGGATGTTGGCCTTTCCACAATGACAAGATGGGTCAAACAACTGCGTGATGAGCGTCAGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGCATTGAAATGGAGAATGAAATATTAAAAAGGCTaccgcgctcttgatgtcagactccctga                                                                                                                                                                                             <  2:245938‑M2/250‑29 (MQ=255)                                                 gCTCAACTGGTTGTTGACCAGCAATACACGGTGGCAGATGCCGCGAAAGCTATGGATGTTGGCCTTTCCACAATGACAAGATGGGTCAAACAACTGCGTGATGAGCGTCAGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGCATTGAAATGGAGAATGAAATATTAAAAAGGCTaccgcgctcttgatgtcagactccctgaacagt                                                                                                                                                                                        <  1:314314‑M2/251‑34 (MQ=255)                                                    cAACTGGTTGTTGACCAGAAATACACGGTGGCAGATGCCGCGAAAGCTATGGATGTTGGCCTTTCCACAATGACAAGATGGGTCAAACAACTGCGCGATGAGCGTCAGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGCATTGAAATGGAGAATGAAATATTAAAAAGGCTaccgcgctcttgatgtcagactccctgaacagttct                                                                                                                                                                                     <  1:114180‑M2/251‑37 (MQ=255)                                                         ggTTGTTGACCAGAAATACACGGTGGCAGATGCCGCGAAAGCTATGGATGTTGGCCTTTCCACAATGACAAGATGGGTCAAACAACTGCGTGATGAGCGTCAGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGCATTGAAATGGAGAATGAAATATTAAAAAGGCTaccgcgctcttgatgtcagactccctgaacagttctcga                                                                                                                                                                                  >  1:81717‑M2/1‑210 (MQ=255)                                                                       aaTACACGGTGGCAGATGCCGCGAAAGCTATGGATGTTGGCCTTTCCACAATGACAAGATGGGTCAAACAACTGCGTGATGAGCGTCAGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGCATTGAAATGGAGAATGAAATATTAAAAAGGCTaccgcgctcttgatgtcagactccctgaacagttctcgataatcgggaaactc                                                                                                                                                                    <  1:229638‑M2/249‑54 (MQ=255)                                                                         tACACGGTGGCAGATGCCGCGAAAGCTCTGGATGTTGGCCTTTCCACAATGACAAGATGGGTCAAACAACTGCGTGATGAGCGTCAGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGCATTGAAATGGAGAATGAAATATTAAAAAGGCTaccgcgctcttgatgtcagactccctgaacagttctcgataatcgggaaactcag                                                                                                                                                                  <  1:386843‑M2/249‑56 (MQ=255)                                                                            acGGTGGCAGATGCCGCGAAAGCTATGGATGTTGGCCTTTCCACAATGACAAGATGGGTCAAACAACTGCGTGATGAGCGTCAGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGCATTGAAATGGAGAATGAAATATTAAAAAGGCTaccgcgctcttgatgtcagactccctgaacagttctcgataatcgggaaactcagcgcgc                                                                                                                                                             <  2:53756‑M2/251‑61 (MQ=255)                                                                             cGGGGGCAGCTGCCGCGAAAGCTATGGATGTTGGCCTTTCCACAATGACAAGATGGGTCAAACAACTGCGTGATGAGCGTCAGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGCATTGAAATGGAGAATGAAATATTAAAAAGGCTaccgcgctcttgatgtcagactccctgaacagttctcgataatcgggaaactcaaagcgc                                                                                                                                                             <  2:224656‑M2/250‑61 (MQ=255)                                                                                            cgAAAGCTATGGATGTTGGCCTTTCCACAATGACAAGATGGGTCAAACACCTGCGTGATGAGCGTCAGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGTACGCTGAGGAAAAAGCTACAACGCATTGAAATGGAGAATGAAATATTAAAAAGGCTaccgcgctcttgatgtcagactccctgaccagttctcgataatcaggaaaatcaaagcgcattatcctgtgctcac                                                                                                                                             <  2:275076‑M2/251‑77 (MQ=255)                                                                                                    aTGGATGTTGGACTGTCCACAATGACAAGATGGGTCAAACAACTGCGTGATGAGCGTCAGGGCAAAACACCAAACGCCTCTCCGCTAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGATACAACGCATTGAAATGGAGAATGAAAAATTAAAAAGGCAaacgcgctcttgatgtcagactccctgaacagttctagatattcgggaaactcagagcgcattatcatgtggtcaacctctgcc                                                                                                                                     <  2:403175‑M2/251‑85 (MQ=255)                                                                                                        aTGTTGGCCTTTCCACAATGACAAGATGGGTCAAACAACTGCGTGATGAGCGTCAGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGCATTGAAATGGAGAATGAAATATTAAAAAGGCTaccgcgctcttgatgtcagactccctgaacagttctcgataatcgggaaactcagagcgcattatcctgtgtacacactctgccatgt                                                                                                                                 <  1:279449‑M2/251‑89 (MQ=255)                                                                                                            tGGCCTTTCCACAATGACAAGATGGGTCAAACAACTGCGTGATGAGCGTCAGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGCATTGAAATGGAGAATGAAATATTAAAAAGGCTaccgcgctcttgatgtcagactccctgaacagttctcgataatcgggaaactcagagcgcattatcctgtggtcacactctgccatgtgtt                                                                                                                              >  1:317729‑M2/1‑159 (MQ=255)                                                                                                             ggCCTTTCCACAATGACAAGATGGGTCAAACAACTGCGTGATGAGCGTCAGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGCATTGAAATGGAGAATGAAATATTAAAAAGGCAaccgcgctcttgatgtcagactccctgaacagttccggataatcgggaaactcaaagcgcattatcctgtggtcacactctgccatgtgttgg                                                                                                                            <  1:270384‑M2/251‑94 (MQ=255)                                                                                                              gCCTTTCCACAATGACAAGATGGGTCAAACAACTGCGTGATGAGCGTCAGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGCATTGAAATGGAGAATGAAATATTAAAAAGGCTaccgcgctcttgatgtcagactccctgaacagttctcgataatcgggaaactcagagcgcattatcctgtggtcacactctgccatgtgttcgg                                                                                                                           >  1:245938‑M2/1‑157 (MQ=255)                                                                                                               ccTTTCCACAATGACAAGATGGGTCACACAACTGCGTGATGAGCGTCAGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGCATTGAAATGGAGAATGAAATATTAAAAAGGCTaccgcgctcttgatgtcagactccctgaacagttctcgataatcgggaaactcagagcgcattatcctgtggtcacactctgccatgtgttcggg                                                                                                                          <  2:417687‑M2/251‑96 (MQ=255)                                                                                                                   tCCACAATGACAAGATGGGTCAAACAACTGCGTGATGAGCGTCAGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGCATTGAAATGGAGAATGAAATATTAAAAAGGCTaccgcgctcttgatgtcagactccctgaacagttctcgataatcgggaaactcagagcgcattatcctgtggtcacactctgccatgtgttcggggtt                                                                                                                       >  1:293144‑M2/1‑152 (MQ=255)                                                                                                                     cacaATGACAAGATGGGTCAAACAACTGCGTGATGAGCGTCAGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGCATTGAAATGGAGAATGAAATATTAAAAAGGCTaccgcgctcttgatgtcagactccctgaacagttctcgataatcgggaaactcagagcgcattatcctgtggtcacactctgccatgtgttcggggttcat                                                                                                                    >  2:51665‑M2/1‑150 (MQ=255)                                                                                                                         aTGACAAGATTGGTCAAACAACTGCGTGATGAGCGTCAGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGCATTGAAATGGAGAATGAAATATTAAAAAGGCTaccgcgctcttgatgtcagactccctgaacagttctcgataatcgggaaactcagagcgcattatcctgtggtcacactctgccatgtgttcggggttcatcgca                                                                                                                >  1:101230‑M2/1‑146 (MQ=255)                                                                                                                           gACAAGATGGGTCAAACAACTGCGTGACGAGCGTCAGGGCAAAACACCAAAAGTCTCTCCGATAACACCAGAACAAAGCGAAATACGGAAGCTGAGGAAAAAGCTACAACTCATTTAAATGGAGAATGAAATATTAAAAAGGCTaccgcgttcttgatgtcagactccctgaacagtcctcgataagcgcgaaactctgagcgcattatcctgtggtcaccctctgccttgtgttcggggttcatctcag                                                                                                               >  2:174889‑M2/1‑144 (MQ=255)                                                                                                                                       cAAACAACTGCGTGATGAGCGTCAGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGCATTGAAATGGAGAATGAAATATTAAAAAGGCTaccgcgctcttgatgtcagactccctgaacagttctcgataatcgggaaactcagagcgcattatcctgtggtcacactctgccatgtgttcggggttcatcgcagcagctacagatac                                                                                                  >  1:449792‑M2/1‑132 (MQ=255)                                                                                                                                          acaacTGCGTGATGAGCGTCAGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCGACAACGCATTGAAATGGAGAATGAAATATTAAAAAGGCTaccgcgctcttgatgtcagactccctgaacagttctcgataatcgggaaactcagagcgcattatcctgtggtcacactctgccatgtgttcggggttcatcgcagcagctacagatactgg                                                                                               >  2:314314‑M2/1‑129 (MQ=255)                                                                                                                                            aacTGCGTGATGAGCGTCAGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGCATTGAAATGGAGAATGAAATATTAAAAAGGCTaccgcgctcttgatgtcagactccctgaacagttctcgataatcgggaaactcagagcgcattatcctgtggtcacactctgccatgtgttcggggttcatcgcagcagctacagatactggaa                                                                                             >  1:280190‑M2/1‑127 (MQ=255)                                                                                                                                            aacTGCGTGATGAGCGTCAGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGCATTGAAATGGAGAATGAAATATTAAAAAGGCTaccgcgctcttgatgtcagactccctgaacagttctcgataatcgggaaactcagagcgcattatcctgtggtcacactctgccatgtgttcggggttcatcgcagcagctacagataatggc                                                                                              <  2:280190‑M2/250‑124 (MQ=255)                                                                                                                                                   tgatgaGCGTCAGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAATCGACATACGTAAGCTGAGGAAAAAGCTACAACGCATTGAAATGGAGAATGAAATATTAAAAAGGCTaccgcgctcttgatgtcagactccctgaacagttcccgataatcgggaaactcagagcgcattatcctgtggtcacactctgccatgtgttcggggttcatcgcagcggctacagatactggaaaaaccgg                                                                                      <  2:427201‑M2/251‑132 (MQ=255)                                                                                                                                                       gaGCGTCAGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGCATTGAAATGGAGAATGAAATATTAAAAAGGCTaccgcgctcttgatgtcagactccctgaacagttcacgataatcgggaaactcagagcgcattatcctgtggtcacactctgccaggtgttcggggttcatcgcagcagctacagatactggaaaaaccgtcctg                                                                                  >  1:417687‑M2/1‑116 (MQ=255)                                                                                                                                                         gCGTCAGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGCATTGAAATGGAGAATGAAATATTAAAAAGGCTaccgcgctcttgatgtcagactccctgaacagttctcgataatcgggaaactcagagcgcattatcctgtggtcacactctgccatgtgttcggggttcatcgcagcagctacagatactggaaaaaccgtcctgaa                                                                                >  1:437738‑M2/1‑114 (MQ=255)                                                                                                                                                              aGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGCATTGAAATGGAGAATGAAATATTAAAAAGGCTaccgcgctcttgatgtcagactccctgaacagttctcgataatcgggaaactcagagcgcattatcctgtggtcacactctgccatgtgttcggggttcatcgcagcagctacagatactggaaaaaccgtcctgaaaaacc                                                                           >  1:345417‑M2/1‑109 (MQ=255)                                                                                                                                                                     aaCTCCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGCATGGAAATGGAGAATGAAATATTAAAAAGGCTaccgcgctcttgatgtcagactccctgaacagttctcgataatcgggaaactcagagcgcattatcctgtggtcacactctgccatgtgttcggggttcatcgcagcagctacagatactggaaaaaccgtcctgaaaaaccagacggc                                                                    >  2:229638‑M2/1‑102 (MQ=255)                                                                                                                                                                           aaaaGCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTAAAAAGCATTGAAATGGAGAATGAAATATTAAAAAGGCTaccgcgctcttgatgtcagactccctgaacagttctcgataatcgggaaactcagagcgcattatcctgtggtcacactctgccatgtgttcggggttcatcgcagcagctacagatactggaaaaaccgtcctgaaaaaccaaacggcagacgg                                                              <  1:342424‑M2/251‑156 (MQ=255)                                                                                                                                                                                     ccGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGCATTGAAATGGAGAATGAAATATTAAAAAGGCTaccgcgctcttgatgtcagactccctgaacagttctcgataatcgggaaactcagagcgcattatcctgtggtcacactctgccatgtgttcggggttcatcgcagcagctacagatactggaaaaaccgtcctgaaaaaccagacggcagacgggctgtatta                                                     >  1:53756‑M2/1‑86 (MQ=255)                                                                                                                                                                                             acCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGCATTGAAATGGAGAATGAAATATTAAAAAGGCTaccgcgctcttgatgtcagactccctgaacagttctcgataatcgggaaactcagagcgcattatcctgtggtcacactctgccatgtgttcggggttcatcgcagcagctacagatactggaaaaaccgtcctgaaaaaccagacggcagacgggctgtattacgcagtc                                              <  1:60738‑M2/249‑172 (MQ=255)                                                                                                                                                                                             acCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGCATTGAAATGGAGAATGAAATATTAAAAAGGCTaccgcgctcttgatgtcagactccctgaacagttctcgataatcgggaaactcagagcgcattatcctgtggtcacactctgccatgtgttcggggttcatcgcagcagctacagatactggaaaaaccgtcctgaaaaaccagacggcagacgggctgtattacgcagtc                                              >  2:60738‑M2/1‑78 (MQ=255)                                                                                                                                                                                                ggaACAAATCGAAATACGTACGCTGAGGAAAAAGCTACAACGCATTGAAATGGAGAATGAAATATTAAAAAGGCTaccgcgctcttgatgtcggactccctgaacagttctcgatattcgggaaactcagagagcattatcctgtggtcacactctgccatgtgttcggggttcatcgcagcagctacagatactggcaaaaccgtcctgaagaaccagccggcagacgggctgaattacgagatcaggt                                          <  2:95146‑M2/249‑176 (MQ=255)                                                                                                                                                                                                       aTCGAAATACGTAAGCTGAGGAAAAAGCTACAACGCATTGAAATGGAGAATGAAATATTAAAAAGGCTaacgcgctcttgatgtcagactccttgaacagttctcgataatcgggaaactcagagcgtattaccctgtggtcacactctgccatgtgttcggggttcatcgcagcagctacagatactggaaaaaccgtcctgaaaaaccagacggcagacgggctgtattacgtagtcaggtacttgagc                                  >  1:95146‑M2/1‑68 (MQ=255)                                                                                                                                                                                                         cGAGATACGTAAGATGAGGAAAAAGCTACAACGCATTGAAATGGAGAATGAAATATTAAAAAGGCTaccgcgctcttgatgtcagacacccagaacagttctcgataatcgggaaactcagggcgcattatcctgtggtaacactctgccatgtgttcggggttcatcgcagcagctacagatactggaaaaaccgtcctgaaaaaccagacggcagccgtgctgcattacgcagtcaggaacttgagct                                 <  2:75898‑M2/250‑185 (MQ=255)                                                                                                                                                                                                                 gTAAGCTGAGGAAAAAGCTACAACGCATTGAAATGGAGAATGAAATATTAAAAAGGCTaccgcgctcttgatgtcagactccctgaacagttctcgataatcgggaaactcagagcgcattatcctgtggtcacactctgccatgtgttcggggttcatcgcagcagctacagatactggaaaaaccgtcctgaaaaaccagacggcagacgggctgtattacgcagtcaggtacttgagctacatggcat                        <  1:376622‑M2/251‑194 (MQ=255)                                                                                                                                                                                                                 gTAAGCTGAGGAAAAAGCTACAACGCATTGAAAGGGAGAATGAAATATTAAAAAGGCTaccgcgctcttgatgtcagactccctgaacagttctctataatcgggaaactcagagcgcattatcctgtggtcacactctgccatgtgtgcggggttcatcgcagcagctacagattctggaaaaaccgtccttaaaaaccagacggcagacgtgctgtattacgcagtcaggtacttgagctacatggcat                        >  1:463123‑M2/1‑58 (MQ=255)                                                                                                                                                                                                                  tAAGCTGAGGAAAAAGCTACAACGCATTGAAATGGAGAATGAAATATTAAAAAGGCTaccgcgctcttgatgtcagactccctgaacagttctcgataatcgggaaactcagagcgcattatcctgtggtcacactctgccatgtgttcggggttcatcgcagcagctacagatactggaaaaaccgtcctgaaaaaccagacggcagacgggctgtattacgcagtcaggtacttgagctacatggcatc                       <  1:366332‑M2/251‑195 (MQ=255)                                                                                                                                                                                                                           gAAAAAGCTACAACGCATTGAAATGGAGAATGAAATATTAAAAAGGCTaccgcgctcttgatgtcagactccatgaacagttctcgataatcgggaaactcagagcgcattatcctgtggtcacactctgccatgtgttcggggttcatcgcagcagctacagatactggaaaaaccgtcctgaaaaaccagacggcagacgggctgtattacgcagtcaggtacttgcgctacatggcatcagccacggt              <  1:304605‑M2/251‑204 (MQ=255)                                                                                                                                                                                                                              aaaGCTACAACGCATTGACATGGAGAATGAAATATTAAAAAGGCTaccgcgctcttgatgtcagactccctgaacagttctcgataatcgggaaactcagagcgcattatcctgtggtcacactctgccatgtgttcggggttcatcgcagcagctacagatactggaaaaaccgtcctgaacaaccagacggcagccgggctgtattacgcagtcaggtacttgagctacatggcatcagccacggatcg           <  2:189247‑M2/251‑207 (MQ=255)                                                                                                                                                                                                                                       aCGCATTGAAATGGAGAATGAAATATTAAAAAGGCTaccgcgctcttgatgtcagactccctgaacagttctcgataatcgggaaactcagagcgcattagcctgtggtcacactctgccatgtgttcggggttcatcgcagcagctacagatactggaaaaaccgtcctgaaaaaccagacggcagacgggctgtattacgcagtcaggtacttgagctacatggcatcagccacggttcggccggagca  >  2:386843‑M2/1‑36 (MQ=255)                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                        GCTCCAATGAAAAAAAGAAATTTCAGCGCAGAGTTTAAACGCGAATCCGCTCAACTGGTTGTTGACCAGAAATACACGGTGGCAGATGCCGCGAAAGCTATGGATGTTGGCCTTTCCACAATGACAAGATGGGTCAAACAACTGCGTGATGAGCGTCAGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGGATTGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAGATTCAATCTGTCAATGCAACACCCCTTTCAATTATCTCTTTCGGTGTTTTGAACTTCAGTGTCTTTCTCGGTCTGTTGTTTAGCTGAGCAGCAACCAGATCTAGTTCATGTTGAGTATATTGGGCAAGACATGTCTTTTTAGGAAAGTACTGCCGAATTAGCCCATTTGTGTTCTCATTTGTTCCCCGCTGCCAAGGACTCTGAGGAT  >  NC_000913/4507191‑4507671

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑