Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,496,168 G→T G14G (GGC→GGA)  ahr ← NADPH‑dependent aldehyde reductase Ahr

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,496,1680GT90.6% 161.5 / ‑0.0 67G14G (GGC→GGA) ahrNADPH‑dependent aldehyde reductase Ahr
Reads supporting (aligned to +/- strand):  ref base G (2/4);  new base T (29/29);  total (31/36)
Fisher's exact test for biased strand distribution p-value = 6.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTACGCGCCGTCCAGCCAATCCCGACACGCTGACCGACCTGCAAACCTTTATCCTGCGCGGCGCTCCCGAGTGCCACCACGCGCCCAATCACCTCATGCCCGGCAACCAGCGGATATTGTGAAAATCCCCATTCGTTATCGATCATCGACAGATCGGAATGGCAGATCCCGCAGTAATCCACCTGCACTTCAACATCTTGTGGCCTCAGCTCACCGGGATCGTACTCATAAACTTCCAGTTCGCCGCCCGCTTCTTTTGCGGCATAGCTTTTTATCATCGACATTTTTTGGTCCTTCTCTGGTGTTGTTTGGGAAGTGTAGAGCATGGCAGGGCGGGGTGCCTGGAGTGTGACAAAGGTTACACATCGCTGTATGCAATGCTGAAAATTTCAGCACTTAGCGAGGTGCGAGCAAGCTGGCGCTTGCATGGTGGCGTGCGACAGGTATAATCCACAACGTTTTCCGCATACCTCTTCAGTGCCGAAGTG  >  NC_000913/4495926‑4496413
                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                     
cTACGCGCCGTCCAGCCAATCCCGACACGCTGACCGACCTGCAAACCTTTATCCTGCGCGGCGCTCCCGCGTGCCACCACGCGCCCAATCACCTCATGCCCGGCAACCAGCGGATATTGTGAAAATCCCCATTCGTTATCGATCATCGACAGATCGGAATGGCAGATCCCGCAGTAATCCACCTGCACTTCACCATCTTGTGGCCTCATCTCACCGGGATCGTACTCATAAACTTCAAGTTCTccgcccgc                                                                                                                                                                                                                                               >  2:353490/1‑251 (MQ=255)
  aCGCGCCGTCCAGCCAATCCCGACACGCTGACCGAACTGCAAACCTTTATCCTGCGCGGCGCTCCCGAGTGCCACCACGCGCCCAATCACCTCATGCCCGGCAACCAGCGGATATTGTGAAAATCCCCATTCGTTATCGATCATCGACAGATCGGAATGGCAGATCCCGCAGTAATCCACCTGCACTTCAACATCTTGTGGCCTCAGCTCACCGGGATCGTACTCATAAACTTCCAGTTCTCCGCCCGctt                                                                                                                                                                                                                                             <  1:230692/251‑1 (MQ=255)
    gcgcCGTCCAGCCAATCCCGACACGCTGACCGACCTGCAAACCTTTATCCTGCGCGGCGCTCCCGAGTGCCACCACGCGACCAATCAACTCATGCCCGGCAACCAGCGGATATTGTGAAAATCACCATTCGTTATCGATAATCGACAGATCGGAATGGCAGATCCCGCAGTAATCCACCTGCACTTCAACATCTTGTGGCCTCAGCTCACCGGGATCGTACTCATAAACTTCCAGTTCGCCGCACGcttct                                                                                                                                                                                                                                           >  1:201871/1‑251 (MQ=255)
     cgcCGTCCAGCCAATCCCGACACGCTGACCGACCTGCAAACCTTTATCCTGCGCGGCGCTCCCGAGTGCCACCACGCGCCCAATCACCTCATGCCCGGCAACCAGCGGATATTGTGAAAATCCCCATTCGTTATCGATCATCGACAGATCGGAATGGCAGATCCCGCAGTAATCCACCTGCACTTCAACATCGTGTGGCCTCAGCTCACCGGGATCGTACTCATAAACTTCCAGTTCTCCGCCCGcttctt                                                                                                                                                                                                                                          <  1:41273/251‑1 (MQ=255)
        cGTCCTGCCACTCACGACTCGCTTACCGACCTGCAAACCTTTAGACAGCGCGGCGCTCCCTAGTGCAACTACGCGCCCATGCGCCTAATGCCCGGCAACCAGCGGATATGGTGAAAATCCCCATTCTTTATCGATCATCGAAAGAGCGGAAGGGCAGATCCCGCAGCAATCCACCTGCACTTCAACATCTTGTGGCCTCAGCTCTCCGGGATCGGACTCATAAACTTCCAGATCTCCGCCCGCTTCTTTTg                                                                                                                                                                                                                                       <  2:33775/251‑1 (MQ=255)
         gTCCAGCCAATCCCGACACGCTGACCGACCTGCAAACCTTTATCCTGCGCGGCGCTCCCGAGTGCCACCACGCGCCCAATCACCTCATGCCCGGCAACCAGCGGATATTGTGAAAATCCCCATTCGTTATCGATCATCGACAGATCGGAATGGCAGATCCCGCAGTAATCCACCTGCACTTCAACATCTTGTGGCCTCAGCTCACCGGGATCGTACTCATAAACTTCCAGTTCTCCGCCCGCTTCTTTTGc                                                                                                                                                                                                                                      <  1:438112/251‑1 (MQ=255)
           ccAGCCAATCCCGACACGCTGACCGACCTGCAAACCTTTATCCTGCGCGGCGCTCCCGAGTGCCACCACGCGCCCAATCACCTCATGCCCGGCAACCAGCGGATATTGTGAAAATCCCCATTCGTTATCGATCATCGACAGATCGGAATGGCAGATCCCGCAGTAATCCACCTGCACTTCAACATCTTGTGGCCTCAGCTCACCGGGATCGTACTCATAAACTGCCAGTTCTCCGCCCGCTTCTTTTGCgg                                                                                                                                                                                                                                    <  1:391217/251‑1 (MQ=255)
                              tGACCGACCTGCAAACCTTTATCCTGCGCGGCGCTCCCGAGTGCCACCACGCGCCCAATCACCTCATGCCCGGCAACCAGCGGATATTGTGAAAATCCCCATTCGTTATCGATCATCGACAGATCGGAATGGCAGATCCCGCAGTAATCCACCTGCACTTCAACATCTTGTGGCCTCAGCTCACCGGGATCGTACTCATAAACTTCCAGTTCTCCGCCCGCTTCTTTTGCGGCATAGCTTTTTATCATCg                                                                                                                                                                                                                  >  1:276491/1‑250 (MQ=255)
                                    accTGCAAACCTTTATCCTGCGCGGCGCTCCCGAGTGCCACCACGCGCCCAATCACCTCATGCCCGGCAACCAGCGGATATTGTGAAAATCCCCATTCGTTATCGATCATCGACAGATCGGAATGGCAGATCCCGCAGTAATCCACCTGCACTTCAACATCTTGTGGCCTCAGCTCACCGGGATCGTACTCATAAACTTCCAGTTCTCCGCCCGCTTCTTTTGCGGCATAGCTTTTTATCATCGACAtttt                                                                                                                                                                                                           >  1:284180/1‑251 (MQ=255)
                                        gCAAACCTTTCTCCTGCGCGGCGCCCCCGAGTGCCCCCACGCGCCCAATCTCCTCAGGCCCGGCAACCAGCGGATATTGGGAAAATCCCCATTCGTTATCGATCATCGACAGATCGGAATGGCAGATCCCGCAGTAATCCACCTGCACTTCAACATCTTGTGGCCTCAGCTCACCGGGATCGTACTCATAAACTTCCAGTTCTCCGCCCGCTTCTTTTGCGGCATAGCGTTTTATCATCGACAtttttt                                                                                                                                                                                                         <  2:197314/249‑1 (MQ=255)
                                        gCAAACCTTTATCCTGCGCGGCGCTCCCGAGTGCCACCACGCGCCCAATCACCTCATGCCCGGCAACCAGCGGATATTGTGAAAATCCCCATTCGTTATCGATCATCGACAGATCGGAATGGCAGATCCCGCAGTAATCCACCTGCACTTCAACATCTTGTGGCCTCAGCTCACCGGGATCGTACTCATAAACTTCCAGTTCTCCGCCCGCTTCTTTTGCGGCATAGCTTTTTATCATCGACATTTTTTgg                                                                                                                                                                                                       <  1:499184/251‑1 (MQ=255)
                                               tttATCCTGCGCGGCGCTCCCGAGTGCCACCACGCGCCCAATCACCTCATGCCCGGCAACCAGCGGATATTGTGAAAATCCCCATTCGTTATCGATCATCGACAGATCGGAATGGCAGATCCCGCAGTAATCCACCTGCACTTCAACATCTTGTGGCCTCAGCTCACCGGGATCGTACTCATAAACTTCCAGTTCTCCGCCCGCTTCTTTTGCGGCATAGCTTTTTATCATCGACATTTTTTGGTCCTtct                                                                                                                                                                                                >  2:223930/1‑251 (MQ=255)
                                                ttacccTGCCCGGCGCTCCCGAGGTCCACCACGCGCCCAATCCCCTCATGCCCGGCAACCAGCGGATATTGTGAAAATCCCCATTCGTTATCGATCATCGACAGATCGGAATGGCAGATCCCGCAGTAATCCACCTGCACTTCAACATCTTGTGGCCTCAGCTCACCGGGATCGTACTCATAAACTTCCAGTTCTCCGCCCGCTTCTTTTGCGGCATAGCTTTTTATCATCGACATTTTTTGGTCCTtc                                                                                                                                                                                                 <  2:408174/245‑1 (MQ=255)
                                                       gcgcGGCGCTCCCGAGTGCCACCACGCGCCCAATCACCTCAGGCCCGGCAACCAGCGGATATTGTGAAAATCCCCATTCGTTATCGATCATCGACAGATCGGAATGGCAGATCCCGCAGTAATCCACCTGCACTTCAACATCTTGTGGCCTCAGCTCACCGGGATCGTACTCATAAACTTCCAGTTCTCCGCCCGCTTCTTTTGCGGCATAGCTTTTTATCATCGACATTTTTTGGTCCTTCTCTGgtg                                                                                                                                                                                          <  1:140629/249‑1 (MQ=255)
                                                        cgcgGCGCTCCCGAGTGCCACCACGCGCCCAATCACCTCATGCCCGGCAACCAGCGGATATTGTGAAAATCCCCATTCGTTATCGATCATCGACAGATCGGAATGGCAGATCCCGCAGTAATCCACCTGCACTTCAACATCTTGTGGCCTCAGCTCACCGGGATCGTACTCATAAACTTCCAGTTCTCCGCCCGCTTCTTTTGCGGCATAGCTTTTTATCATCGACATTTTTTGGTCCTTCTCTGGtgttg                                                                                                                                                                                       >  1:62209/1‑251 (MQ=255)
                                                        cgcgGCGCTCCCGAGTACCACCACGCGCCCAATCACCTCACGTCCGGCTACCCGCGGATAATGTGACAATCCCCATTCGTTATCGTTCATCGACAGATCGGAATGGCAGTTCCCGCAGTAATCCACCTGCACTTCAACATCTTTTGGCCTCAGCTCACCGTGATCGTACTCATAAACTTCCAGTTCTCCGCCCGCTTCTTTTGCGGCTTCGCTTTTTATCATCGACATTTTTTGGTCCTTCTCTGgttgtg                                                                                                                                                                                       >  2:187384/1‑247 (MQ=255)
                                                         gcggcgCTCCCGAGTGCCACCACGCGCCCAATCACCACATGCCCGGCAACCAGCGGATATTGTGAAAATCCCCATTCGTTATCGATCATCGACAGATCGGAATGGCAGATCCCGCAGTAATCCACCTGCACTTCAACATCTTGTGGCCTCAGCTCACCGGGATCGTACTCATAAACTTCCAGTTCTCCGCCCGCTTCTTTTGCGGCATAGCTTTTTATCATCGACATTTTTTGGTCCTTCTCTGGtgttgt                                                                                                                                                                                      <  1:396983/251‑1 (MQ=255)
                                                             cgcTCCCGAGTGCCACCACGCGCCCAAGCACCTCAGGCCCGGCAACCAGCGTATATTGTTAAAATCCCCATTCGTTATCGATCAGCGACAGATCGGAATGGCAGATCCCGCAGTAATCCATCTGTACTTCAACATCTTGTGGCCTCAGCTCACCGGGATCTTACGCATAAAGTGCCAGTTCTCCGCCCGCTTCTTTTTCGGCATAGCGTTTTATCAGCGACATTTTTTGGTCCTTCTCTGGTGTTGTTTgg                                                                                                                                                                                  <  1:457795/251‑1 (MQ=255)
                                                                   cGAGTGCCACCACGCGCCCAAACACCTCAGGCCCGGCACCCAGCGGCTATTGTGAAAATCCCCATCCGTTATCGGTCATCGACAGATCGGAATGGCAGTCCCCGCAGTAATCCACCTGCACTTCATCACCTTGTGGCCTCAGCTCACCGGGATCGTACTCATAAACTTCCAGTTCTCCGCCCGCTTCTTTTGCGGCATAGCTTTTTATCATCGACATTTTTTGGTCCTTCTCTGGTGTTGTTTGGGAAgtg                                                                                                                                                                            <  2:389432/251‑1 (MQ=255)
                                                                     agagCCACCACGCGCCCAATCACCTCATGCCCGGCAACCAGCGGATATTGTGCAAATCCCCATTCGTTATCGATCATCGACAGATCGGAATGGCAGATCCCGCAGTAATCCACCTGCACTTCAACATCTTGTGGCCTCAGCTCACCGGGATCGTACTCATAAACTTCCAGTTCTCCGCCCGCTTCTTTTGCGGCATAGCTTTTTATCATCGACATTTTTTGGTCCTTCTCTGGGGTTGTTTGGGAAgtgt                                                                                                                                                                           >  2:285541/4‑250 (MQ=255)
                                                                               cgcgCCCAATCACCTCATGCCCGGCAACCAGCGGCTATTGTGAAAATCCCCATTCGTTATCGATCATCGACAGATCGGAATGGCAGATCCCGCAGTAATCCACCTGCACTTCAACATCTTGTGGCCTCAGCTCACCGGGATCGTACTCATAAACTTCCAGTTCTCCTCCCGCTTCTTTTGCGGCATAGCTTTTTATCATCGACATTTTTTGGTCCTTCTCTGGTGTTGTTTGGGAAGTGTAGAGCATGGc                                                                                                                                                                 >  2:282242/1‑250 (MQ=255)
                                                                                   cccAATCCCCTCATGCCCGGCAACCAGCGGATATTGTGAAAACCCCCATTCGTTATCGATCATCGACAGATCGGAATGGCAGATCCCGCAGTAATCCACCTGCACTTCAACATCTTGTGGCCTCAGCTCACCGGGATCGTACTCATAAACTTCCAGTTCTCCGCCCGCGTCtttt                                                                                                                                                                                                                                        <  2:354945/175‑1 (MQ=255)
                                                                                   cccAATCACCTCATGCCCGGCAACCAGCGGATATTGTGAAAATCCCCATTCGTTATCGATCATCGACAGATCGGAATGGCAGATCCCGCAGTAATCCACCTGCACTTCAACATCTTGTGGCCTCAGCTCACCGGGATCGTACTCATAAACTTCCAGTTCTCCGCCCGCTTCtttt                                                                                                                                                                                                                                        >  1:354945/1‑175 (MQ=255)
                                                                                    ccAATCACCTCATGCCCGGCAACCAGCGGATATTGTGAAAATCCCCATTCGTTATCGATCATCGACAGATCGGAATGGCAGATCCCGCAGTAATCCACCTGCACTTCAACATCTTGTGGCCTCAGCTCACCGGGATCGTACTCATAAACTTCCAGTTCTCCGCCCGCTTCTTTTGCGGCATAGCTTTTTATCATCGACATTTTTTGGTCCTTCTCTGGTGTTGTTTGGGAAGTGTAGAGCATGGCAGGGCg                                                                                                                                                           >  1:64153/1‑251 (MQ=255)
                                                                                         cACCTCATGCCCGGCAACCAGCGGATATTGTGAAAATCCCCATTCGTTATCGATCATCGACAGATCGGAATGGCAGATCCCGCAGTAATCCACCTGCACTTCAACATCTTGTGGCCTCAGCTCACCGGGATCGTACTCATAAACTTCCAGTTCTCCGCCCGCTTCTTTTGCGGCATAGCTTTTTATCATCGACATTTTTTGGTCCTTCTCTGGTGTTGTTTGGGAAGTGTAGAGCATGGCAGGGCGGGGTg                                                                                                                                                      <  1:223930/251‑1 (MQ=255)
                                                                                         cACCTCATGCCCGGCAACCAGCGGATATTGTGAAAATCCCCATTCGTTATCGATCATCGACAGATCGGAATGGCAGAGCCCGCAGTAATCCACCTGCACTTCAACATCTTGTGGCCTCAGCTCACCGGGATCGTACTCATAAACTTCCAGTTCTCCGCCCGCTTCTTTTGCGGCATAGCTTTTTATCATCGACATTTTTTGGTCCTTCTCTGGTGTTGTTTGGGAAGTGTAGAGCATGGCAGGGCGGGGt                                                                                                                                                       <  1:244269/250‑1 (MQ=255)
                                                                                           ccTCATGCCCGGCAACCAGCGGATATTGTGAAAATCCCCATTCGTTATCGATCATCGACAGATCGGAATGGCAGATCCCGCAGTAATCCACCTGCACTTCAACATCTTGTGGCCTCAGCTCACCGGGATCGTACTCATAAACTTCCAGTTCTCCGCCCGCTTCTTTTGCGGCATAGCTTTTTATCATCGACATTTTTTGGTCCTTCTCTGGTGTTGTTTGGGAAGTGTAGAGCATGGCAGGGCGGGGTGcc                                                                                                                                                    <  1:128712/251‑1 (MQ=255)
                                                                                                tGCCCGGCAACCAGCGGATATTGTGAAAATCCCCATTCGTTATCGATCATCGACAGATCGGAATGGCAGATCACGCAGTAATCCACCTGCACTTCAACATCTTGTGGCCTCAGATCACCGGGATCGTACTCATAAACTTCCAGTTCTCCGCCCGCTTCTTTTGCGGCATAGCTTTTTATCATCGACATTTTTTGGTCCTTCTCTGGTGTTGTTTGGGAAGTGTAGAGCATGGCAGGGCGGGGAGCCTgg                                                                                                                                                 >  2:199249/1‑249 (MQ=255)
                                                                                                 gCCCGGCAACCAGCGGATATTGTGAAAATCCCCATTCGTTATCGATCATCGACAGATCGGAATGGCAGATCCCGCAGTAATCCACCTGCACTTCAACATCTTGTGGCCTCAGCTCACCGGGATCGTACTCATAAACTTCCAGTTCTCCGCCCGCTTCTTTTGCGGCATAGCTTTTTATCATCGACATTTTTTGGTCCTTCTCTGGTGTTGTTTGGGAAGTGTAGAGCATGGCAGGGCGGGGTGCCTGGAg                                                                                                                                               <  1:309687/250‑1 (MQ=255)
                                                                                                 gCCCGGCAACCAGCGGATATTGTGAAAATCCCCATTCGTTATCGATCATCGACAGATCGGAATGGCAGATCCCGCAGTAAGCCACCTGCACTTCAACATCTTGTGGCCTCAGCGCACCGGGATCGTACTCATAATCTTCCAGTTCGCCGCCCGCTTCTTTTGCGGCATAGCTTTTTATCATCGACATTTTTTGGTCCTTCTCTGGTGTTGTTTGGGAAGTGTAGAGCATGGCAGGGCGGGGTGCCTGGAg                                                                                                                                               <  1:248737/250‑1 (MQ=255)
                                                                                                   ccGGCAACCAGCGGATATTGTGAAAATCCCCATTCGTTATCGATCATCGACAGATCGGAATGGCAGATCCCGCAGTAATCCCCCTGCACTTCAACATCTTGTGGCCTCAGCTCACCGGGATCGTACTCATAAACTTCCAGTTCTCCGCCCGCTTCTTTTGCGGCATAGCTTTTTATCATCGACATTTTTTGGTCCTTCTCTGGTGTTGTTTGGGAAGTGTAGAGCATGGCAGGGCGGGGTGCCTGGAgtgt                                                                                                                                            >  1:58284/1‑251 (MQ=255)
                                                                                                     ggCAACCAGCGCATTTTGTGAAAATCCCCATTCGTTATCGACCATCGACAGTTCGGAATGCCCGATCCCGCATAAATCCCTCTGCACTTCAACACCTTTTGGCCTCAGCTCTCCGGGAGCGTACTCTTAAACTTCCAGTTCTCCGCCCGCTTCTTTTGCGGCATAGCTTTTTATCATCGACATTTTTTGGTCCGTCTCTGGTGTTGTTTGGGAAGTGTAGAGCATGGCAGGGCGGGTTGCCTGGAGTGTGa                                                                                                                                          <  2:401009/251‑1 (MQ=255)
                                                                                                         aCCAGCGGATATTGTGAAAATCCCCATTCGTTATCGATCATCGACAGATCGGAATGGCAGATCCCGCAGTAATCCACCTGCACTTCAACATCTTGTGGCCTCAGCTCACCGGGATCGTACTCATAAACTTCCAGTTCTCCGCCCGCTTCTTTTGCGGCATAGCTTTTTATCATCGACATTTTTTGGTCCTTCTCTGGTGTTGTTTGGGAAGTGTAGAGCATGGCAGGGCGGGGTGCCTGGAGTGTGACaaa                                                                                                                                      <  1:311116/251‑1 (MQ=255)
                                                                                                               ggATATTGTGAAAATCCCCATTCGTTATCGATCATCGACAGATCGGAATGGCAGATCCCGCAGTAATCCACCTGCACTTCAACATCTTGTGGCCTCAGCTCACCGGGATCGTACTCATAAACTTCCAGTTCTCCGCCCGCTTCTTTTGCGGCATAGCTTTTTATCATCGACATTTTTTGGTCCTTCTCTGGTGTTGTTTGGGAAGTGTAGAGCATGGCAGGGCGGGGGGCCTGGAGTGTGACAAAGGTTac                                                                                                                                >  1:5796/1‑251 (MQ=255)
                                                                                                                 atatGGTGAAAATCCCCATTCGTTATCGATCATCGACAGATCGGAATGGCAGATCCCGCAGTAATCCACCTGCACTTCAACATCTTGTGGCCTCAGCTCACCGGGATCGTACTCATAAACTTCCAGTTCTCCGCCCGCTTCTTTTGCGGCATAGCTTTTTATCATCGACATTTTTTGGTCCTTCTCTGGTGTTGTTTGGGAAGTGTAGAGCATGGCAGGGCGGGGTGCCTGGAGTGTGACAAAGGTTacac                                                                                                                              <  2:471246/251‑1 (MQ=255)
                                                                                                                  tatTGTGAAAATCCCCATTCGTTATCGATCATCGACAGATCGGAATGGCAGATCCCGCAGTAATCCACCTGAACTTCAACATCTTGTGGCCTCCGCTCACCGGGATCGTACTCATAAACTTCCAGTTCTCCGCCCGCTTCTTTTGCGGCATAGCTTTTTATCATCGCCATTTTTTGGTCCTTCTCTGGTGTTGTTTGGGAAGTGTAGAGCATGGCAGGGCGGGGTGCCTGGAGTGTGACAAAGGTTaaac                                                                                                                              >  1:463569/1‑247 (MQ=255)
                                                                                                                            aTCCCCATTCGTTATCGATCATCGACAGATCGGAATGGCAGATCCCGCAGTAATCCACCTGCACTTCAACATCTTGTGGCCTCAGCTCACCGGGATCGTACTCATAAACTTCCAGTTCGCCGCCCGCTTCTTTTGCGGCATAGCTTTTTATCATCGACATTTTTTTGTCCTTCTCTGGTGTTGTTTGGGAAGTGTAGAGCATGGCAGGGCGGGGTGCCTGGAGTGTGAAAAAGGTTACACATCGCTGTATg                                                                                                                   >  1:164547/1‑251 (MQ=255)
                                                                                                                                 cATTCGTTATCTACCATCGACAGATCGGAAGGGCAAATCCCGCAGTAATCCTCCGGCACTTCAACTTCTGGTGGCCTCAGCTCCCCGGGATCGTACTCATAAACTTCCAGTTCTCCGCCCGCTTCTTTTGCGGCATAGCTTTTTATCATCGACATGTTTTGGTCCTTCTCTGGTGTTGTTTGGGAAGTGTAGAGCATGGCAGGGCGGGGTGCCTGGAGTGTGACAAAGGTTACACATCGCTGTATGCAATg                                                                                                              <  2:301189/251‑1 (MQ=255)
                                                                                                                                     cGTTATCAATCATCCACAGACCGGAAGGGCAGATCCCGCAGTAATCCCCCTGCCCTTCAACATCTTGTGGCCCCAGCTCACCGGGTCCGTACTCATAAACTTCCAGTTCTCCGCCCGCTTCTTTTGCGGCATAGCTTTTTATCAGCGACATTTTTTGGTCCTTCTCTTGTGTTGTTTGGGAAGTGTAGAGCATGGCAGGGCGGGGTGCCTGGAGTGTGACAAAGGTTACACATCGCTGTATGCAATGCTGa                                                                                                          <  2:301618/251‑1 (MQ=255)
                                                                                                                                         aTCGCTCATTGACAGATCGGCATGGCAGATCCCGCAGGAATCCACCTGCACTTCACCATCTTGTGGCCTCAGCTCACCGTGATCGTACTCATAAACTTACAGTTCTCCGCCCGCTTCTTTTGCGGCATAGCTTTTTATCATCGACATTTTTTGGTCCTTCTCTGGTGTTGTTTGGGAAGTGGAGAACATGGCAGGGCGGGGTGCCGGGATGGTGACAAAGGTTACACATCGCTGTATGccaatctgcaaat                                                                                                      >  2:371423/1‑239 (MQ=255)
                                                                                                                                           cGATCATCGACAGATCGGAATGGCAGATCCCGCAGTAATCCACCTGCACTTCAACATCTTGTGGCCTCAGCTCACCGGGATCGTACTCATAAACTTCCAGTTCTCCGCCCGCTTCTTTTGCGGCATAGCTTTTTATCATCGACATTTTTTGGTCCTTCTCTGGTGTTGTTTGGGAAGTGTAGAGCATGGCAGGGCGGGGTGCCTGGAGTGTGACAAAGGTTACACATCGCTGTATGCAATGCTGAAAAttt                                                                                                    >  1:437516/1‑251 (MQ=255)
                                                                                                                                             atcatcGACAGATCGGAAGGGCAGATCCCGCAGGAATCCACCTGCACTTCAACAGCTGGGGGCCTCAGCTCACCGGGATCGTACTCATAAACGTCCAGTTCGCCGCCCGCTTCTTGTGCGGCATAGCTTTTTATCAGCGACATTTTTTGGTCCTTCTCTGGTGTTGTTTGGGAAGTGTAGAGCATGGCAGGGCGGGGTGCCTGGAGTGTGACAAAGGTTACACATCGCTGTATGCAATGCTGAAAATTTCa                                                                                                  <  2:164547/251‑1 (MQ=255)
                                                                                                                                               catcGACAGATCGGAATGGCAGATCCCGCAGTAATCCACCTGCACTTCAACATCTTGTGGCCTCAGCTCACCGGGATCGTACTCATAAACTTCCAGTTCTCCGCCCGCTTCTTTTGCGGCATAGCTTTTTATCATCGACATTTTTTGGTCCTTCTCTGGTGTTGTTTGGGAAGTGTAGAGCATGGCAGGGCGGGGTGCCTGGAGTGTGACAAAGGTTACACATCGCTGTATGCAATGCTGAAAATTTCAg                                                                                                 <  1:280300/250‑1 (MQ=255)
                                                                                                                                               caccgacagaacagaattccagtttccGCAGTAATCCAACTACACTCCAACATCTTGTAGCCTCCCCTAACCGGGATCTTACTCATAAACTTCCCGTTCTCCGCTCGCCTCTTTTGCGGCATAGCTTTTTATCATCGACATTTTTTCGTCCTTCTCTGGTGTTGCTTGGGTAGTGTAGAGCATGGCAGGGCGGGGTGCCTGGAGCGGGACAAAGGTTACACATCGCTGTGTGCACTACTGCAAAATTCAGc                                                                                                >  2:228127/26‑251 (MQ=255)
                                                                                                                                                  cGACAGATCGGAATGGCATATCCCGCAGTAATCCACCTGCACTTAAACATCTTGTGGCCTCAGCTCACCGGGATCGTACTCATAAACTTCCAGTTCCCCGCCCGCTTCTTTTGCGGCATAGCTTTTTATCATCGACATTTTTTGGTCCTTCTCTGGTGTTGTTTGGGAAGTGTAGAGCATGGCAGGGCGGGGTGCCTGGAGTGTGACAAAGGTTACACATCGCTGTATGCAATGCTGAAAATTTCAGCACt                                                                                             <  1:233880/251‑1 (MQ=255)
                                                                                                                                                       cctCGGAATGGCAGACCCCGCAGTAATCCTCCTGCACTTCAACATCTTGTGGCCGCAGCTCACCGGGATCGTACTCATAAACCTCCAGTTCTCCGCCCGCTTCTTTTGCGGCATAGCTTTTTATCATCGACATTTTTTGGTCCTTCTCTGGTGTTGTTTGGGAAGTGTAGAGCATGGCAGGGCGGGGTGCCTGGAGTGTGACAAAGGTTACACATCGCTGTATGCAATGCTGAAAATTTCAGCACTTAGCg                                                                                        <  2:50248/249‑1 (MQ=255)
                                                                                                                                                        aTCGGAATGGCAGATCCCGCAGGAAGCCACCGGCACTTCAACATCTTGTGGCCTCAGCTCACCGGGATCGTACTCATAAACTTCCAGTTCTCCGCCCGCTTCTTTTGCGGCATAGCTTTTTATCATCGACATTTTTTGGTCCTTCTCTGGTGTTGTTTGGGAAGTGTAGAGCATGGCAGGGCGGGGTGCCTGGAGTGTGACAAAGGTTACACATCGCTGTATGCAATGCTGAAAATTTCAGCACTTAGCgt                                                                                       <  1:76472/251‑2 (MQ=255)
                                                                                                                                                          cGGAATGGCAGATCCCGCAGTAATCCACCTGCACTTCAACATCTTGTGGCCTCAGCTCACCGGGATCGTACTCATAAACTTCCAGTTCTCCGCCCGCTTCTTTTGCGGCATAGCTTTTTATCATCGACCTTTTTTGGTCCTTCTCTGGAGTTGTTTGGGAAGTGTAGAGCATGGCAGGGCGGGGTGCCTGGAGTGTGACAAAGGTTACACATCGCTGTATGCAATGCTGAAAATTTCAGCACTTAGCg                                                                                        >  1:50248/1‑248 (MQ=255)
                                                                                                                                                                         cGCAGTAATCCACCTGCACTTCAACATCTTGTGGCCTCAGCTCACCGGGATCGTACTCATAAACTTCCAGTTCTCCGCCCGCTTCTTTTGCGGCATAGCTTTTTATCATCGACATTTTTTGGTCCTTCTCTGGTGTTGTTTGGGAAGTGTAGAGCATGGCAGGGCGGGGTGCCTGGAGTGTGACAAAGGTTACACATCGCTGTATGCAATGCTGAAAATTTCAGCACTTAGCGAGGAGCGAGCAAGCTGgc                                                                      >  1:438190/1‑251 (MQ=255)
                                                                                                                                                                          gCAGTAATCCACCTGCACTTCAACATCTTGTGGCCTCAGCTCACCGGGATCGTACTCATAAACTTCCAGTTCTCCGCCCGCTTCTTTTGCGGCATAGCTTTTTATCATCGACATTTTTTGGTCCTTCTCTGGTGTTGTTTGGGAAGTGTAGAGCATGGCAGGGCGGGGTGCCTGGAGTGTGACAAAGGTTACACATCGCTGTATGCAATGCTGAAAATTTCAGCACTTAGCGGGGTGCGAGCAAGCTGgcg                                                                     >  2:422048/1‑251 (MQ=255)
                                                                                                                                                                               caccctcctacACTTCATCCTCTTGTGGACTCAGCCCACCGGGTTCTTACTCAGTATCTTCCAGTGCCCCGCCCGCTTCTTTTGCGGCATAGCTTTTTATCATCGACATTTTTTGGTCCTTCTCTGGTGTTGTTTGGGAAGTTTAGAGCATGGCAGTGCGGGGTGCCTGGAGTGTGACAAAGGTTACACATCGCTGTATGCAATGCTGAAAATTTCAGCACTTAGCGAGGTGCGATCAAGCTGGCGCTTGc                                                                <  2:452802/241‑1 (MQ=255)
                                                                                                                                                                               aaTCCACCTGCACTTCAACATCTTGTGGCCTCAGCTCACCGGGATCGTACTCATAAACTTCCAGTTCTCCGCCCGCTTCTTTTGCGGCATAGCTTTTTATCATCGACATTTTTTGGTCCTTCTCTGGTGTTGTTTGGGAAGTGTAGAGCATGGCAGGGCGGGGTGCCTGGAGTGTGACAAAGGTTACACATCGCTGTATGCAATGCTGAAAATTTAAGCACTTAGCGAGGTGCGAGCAAGCTGGCGCTTGc                                                                >  1:44089/1‑251 (MQ=255)
                                                                                                                                                                                      cTGCACTTCAACATCTTGTGGCCTCAGCTCACCGGGATCGTACTCATAAACTTCCAGTTCTCCGCCCGCTTCTTTTGCGGCATAGCTTTTTATCATCGACATTTTTTGGTCCTTCTCTGGTGTTGTTTGGGAAGTGTAGAGCATGGCAGGGCGGGGTGCCTGGAGTGTGACAAAGGTTACACATCGCTGTATGCAATGCTGAAAATTTCAGCACTTAGCGAGGGGAGAGCAAGCTGGCGCTTGCAtggtgg                                                         >  2:204813/1‑251 (MQ=255)
                                                                                                                                                                                       tGCACTTCAACATCTTGTGGCCTCAGCTCACCGGGATCGTACTCATAAACTTCCAGTTCTCCGCCCGCTTCTTTTGCGGCATAGCTTTTTATCATCGCCATTTTTTGGTCCTTCTCTGGTGTTGTTTGGGAAGTGTAGAGCATGGCAGGGCGGGTTGCCTGGAGTGTGACAAAGGTTACACATCGCTGTATGCAATGCTGAAAATTTCAGCCCTTCGCGAGGTCCAAGCAAGCTGGCGCTTGCATGGTGGc                                                        >  1:469434/1‑251 (MQ=255)
                                                                                                                                                                                       tGCACTTCAACATCTTGTGGCCTCAGCTCACCGGGATCGTACTCATAAACTTCCAGTTCTCCGCCCGCTTCTTTTGCGGCATAGCTTTTTATCATCGACATTTTTTGGTCCTTCTCTGGTGTTGTTTGGGAAGTGTAGAGCATGGCAGGGCGGGGTGCCTGGAGTGTGACAAAGGTTACACATCGCTGTATGCAATGCTGAAAATTTCAGCACTTAGCGAGGTGCGAGCAAGCTGGCGCTTGCATGGTGGc                                                        >  1:242476/1‑251 (MQ=255)
                                                                                                                                                                                                aCATCTTGTGGCCTCAGCTCACCGGGATCGTACTCATAAACTTCCAGTTCTCCGCCCGCTTCTTTTGCGGCATAGCTTTTTATCATCGACATTTTTTGGTCCTTCTCTGGTGTTGTTTGGGAAGTGTAGAGCATGGCAGGGCGGGGTGCCTGGAGTGTGACAAAGGTTACACATCGCTGTATGCAATGCTGAAAATTTCAGCACTTAGCGAGGTGCGAGCAAGCTGGCGCTTGCATGGTGGCGTGCGAc                                                 >  1:401254/1‑249 (MQ=255)
                                                                                                                                                                                                                   cACCGGGATCGTACTCATAAACTTCCAGTTCTCCGCCCGCTTCTTTTGCGGCATAGCTTTTTATCATCGACATTTTTTGGTCCTTCTCTGGTGTTGTTTGGGAAGTGTAGAGCATGGCAGGGCGGGGTGCCTGGAGTGTGACAAAGGTTACACATCGCTGTATGCAATGCTGAAAATTTCAGCACTTAGCGAGGTGCGAGCAAGCTGGCGCTTGCATGGTGGCg                                                       >  2:481831/1‑224 (MQ=255)
                                                                                                                                                                                                                   cACCGGGATCGTACTCATAAACTTCCAGTTCTCCGCCCGCTTCTTTTGCGGCATAGCTTTTTATCATCGACATTTTTTGGTCCTTCTCTGGTGTTGTTTGGGAAGTGTAGAGCATGGCAGGGCGGGGTGCCTGGAGTGTGACAAAGGTTACACATCGCTGTATGCAATGCTGAAAATTTCAGCACTTAGCGAGGTGCGAGCAAGCTGGCGCTTGCATGGTGGCg                                                       <  1:481831/224‑1 (MQ=255)
                                                                                                                                                                                                                      cGGGATCGTACTCATAAACTTCCAGTTCTCCGCCCGCTTCTTTTGCGGCATAGCTTTTTATCATCGACATTTTTTGGTCCTTCTCTGGTGTTGTTTGGGAAGTGTAGAGCATGGCAGGGCGGGGTGCCTGGAGTGTGACAACGGTTACACATCGCTGTATGCAATGCTGAAAATTTCAGCACTTAGCGAGGTGCGAGCAAGCTGGCGCTTGCATGGTGGCGTGCGACAGGTATAATCCACAACGTTTTCCg                         >  1:489335/1‑251 (MQ=255)
                                                                                                                                                                                                                         gATCGTACTCATAAACTTCCAGTTCTCCGCCCGCTTCTTTTGCGGCATAGCTTTTTATCATCGACATTTTTTGGTCCTTCTCTGGTGTTGTTTGGGAAGTGTAGAGCATGGCAGGGCGGGGTGCCTGGAGTGTGACAAAGGTTACACATCGCTGTATGCAATGCTGAAAATTTCAGCACTTAGCGAGGTGCGAGCAAGCTGGCGCTTGCATGGTGGCGTGCGACAGGTATAATCCACAACGTTTTCCGCAt                      <  2:64153/251‑1 (MQ=255)
                                                                                                                                                                                                                           tCGTACTCATAAACTTCCAGTTCTCCGCCCGCTTCTTTTGCGGCATAGCTTTTTATCATCGACATTTTTTGGTCCTTCTCTGGTGTTGTTTGGGAAGTGTAGAGCATGGCAGGGCGGGGTGCCTGGAGTGTGACAAAGGTTACACATCGCTGTATGCAATGCTGAAAATTTCAGCACTTAGCGAGGTGCGAGCAAGCTGGCGCTTGCATGGTGGCGTGCGACAGGTATAATCCACAACGTTTTCCGCATAc                    >  1:476121/1‑251 (MQ=255)
                                                                                                                                                                                                                                  cATAAACTTCCAGTTCTCCGCCCGCTTCTTTTGCGGCATAGCTTTTTATCATCGACATTTTTTGGTCCTTCTCTGGTGTTGTTTGGGAAGTGTAGAGCATGGCAGGGCGGGGTGCCTGGAGTGTGACAAAGGTTACACATCGCTGTATGCAATGCTGAAAATTTCAGCACTTAGCGAGGTGCGAGCAAGCTGGCGCTTGCATGGTGGCGTGCGACAGGTATAATCCACAACGTTTTCCGCATACCTCTTc              <  1:27588/250‑1 (MQ=255)
                                                                                                                                                                                                                                  cATAAACTTCCAGTTCTCCGCCCGCTTCGTTTGCGGCATAGCTTTTTATCATCGACATTTTTTGGTCCTTCTCTGGTGTTGTTTGGGAAGTGTAGAGCATGGCAGGGCGGGGTGCCTGGAGTGTGACAAAGGTTACACATCGCTGTATGCAATGCTGAAAATTTCAGCACTTAGCGAGGTGCGAGCAAGCTGGCGCTTGCATGGTGGCGTGCGACAGGTATAATCCACAACGTTTTCCGCATACCTCTTc              <  1:31951/250‑1 (MQ=255)
                                                                                                                                                                                                                                          tCCAGTTCGCCGCCCGCTTCTTTTGCGGCATAGCTTTTTATCAGCGTCATTTGTTGGTCCTTCTCTGGTGTTGTTGGGGAAGTGTATAGCACGGCAGGGCGGGGTGCCTGGAGTGTGACCAAGGTTACTCATCGCTGTATGCAATGCTGAAAATTCCAGCACTTAGCCGGGTGCGAGCAAGCTGGCGCTTGCATTGTGCCGTGCGACAGGTATAATCCACAACGTTTTCCGCATACCTCTTCAGTGCCGa      <  1:316409/250‑1 (MQ=255)
                                                                                                                                                                                                                                           ccAGTTCTCCGCCCGCTTCTTTTGCGGCATAGCTTTTTATCATCGACATTTTTTGGTCCTTCTCTGGTGTTGTTTGGGAAGTGTAGAGCATGGCAGGGCGGGGGGCCTGGAGTGTGACAAAGGTTACACATCGCTGTATGCAATGCTGAAAATTTCAGCACTTAGCGAGGTGCGAGCAAGCTGGCGCTTGCATGGTGGCGTGCGACAGGTATAATCCACAACGTTTTCCGCATACCTCTTCAGTGCCGa      >  1:362769/1‑249 (MQ=255)
                                                                                                                                                                                                                                             aGTTCGCCGCCCGCTTCTTTTGCGGCATAGCTTTTTAACATCGGCATTTTTTGGTCCTTCTCTGGGGTTGTTTGGGAAGTGTAGAGCATGGCAGGGCGGGGTGCCTGGAGTGTGACAAAGGTTACACATCGCTGTATGCAATGCTGAAAATTTCAGCACTTAGCGAGGTGCGAGCAAGCTGGCGCTTGCATGGTGGCGTGCGACAGGTATAATCCACAACGTTTTCCGCATACCTCTTCAGTGCCGAAGTg  <  2:31813/251‑1 (MQ=255)
                                                                                                                                                                                                                                              gTTCCCCGCCCGCTTCTTTTGCGGCATAGCTTTTTATCATCGACATGTTGTGGTCCTTCTCTGGTGGTGTTTGGGAAGTGTAGAGCATGGCAGGGCGGGGTGCCTGGAGTGTGACAAAGGTTACACATCGCTGTATGCAATGCTGAAAATTTCAGCACTTAGCGAGGTGCGAGCAAGCTGGCGCTTGCATGGTGGCGTGCGACAGGTATAATCCACAACGTTTTCCGCATACCGCTTCAGTGCCGAAGTg  <  2:362769/250‑1 (MQ=255)
                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                     
CTACGCGCCGTCCAGCCAATCCCGACACGCTGACCGACCTGCAAACCTTTATCCTGCGCGGCGCTCCCGAGTGCCACCACGCGCCCAATCACCTCATGCCCGGCAACCAGCGGATATTGTGAAAATCCCCATTCGTTATCGATCATCGACAGATCGGAATGGCAGATCCCGCAGTAATCCACCTGCACTTCAACATCTTGTGGCCTCAGCTCACCGGGATCGTACTCATAAACTTCCAGTTCGCCGCCCGCTTCTTTTGCGGCATAGCTTTTTATCATCGACATTTTTTGGTCCTTCTCTGGTGTTGTTTGGGAAGTGTAGAGCATGGCAGGGCGGGGTGCCTGGAGTGTGACAAAGGTTACACATCGCTGTATGCAATGCTGAAAATTTCAGCACTTAGCGAGGTGCGAGCAAGCTGGCGCTTGCATGGTGGCGTGCGACAGGTATAATCCACAACGTTTTCCGCATACCTCTTCAGTGCCGAAGTG  >  NC_000913/4495926‑4496413

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑