Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,476,605	G→A	S390N (AGC→AAC)	yjgL →	protein YjgL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,476,605	0	G	A	95.2%	61.6 / ‑5.6	21	S390N (AGC→AAC)	yjgL	protein YjgL
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (7/13);  minor base T (1/0);  total (8/13)
Fisher's exact test for biased strand distribution p-value = 3.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAAAGGTATACTTGAAGAAATAGATAAGCTCTACGCATTAAATGATCACGGAATTGATAATGACAAAGTAGGTAACAATGAAATTAATGACATCAAAGTTAACCTGTCTCATATTCTTATTGATTCCTTAGATGATGCAAAGGTTAACCTTACACCGGTCATCGATTCGATTCTGGAGACTTTTTCAAAATCCCCATATATTAATGATGTAAGAATACTGGATTGGTGTTTTAATAAAAGCATGCAATATTTTGATGATACTAAAAAGATAAAGCATGCATGCTCCGTAATAAATCATATTAATCTTCGCAGCGATCAGTCTAAAATAGCTGAGACATTATTTTTCAATCTCGATAAAGAACCCTATAAAAATAGCCCTGAATTACAGGGGTTGATTTGGAATAAGTTGGTTGTATATGTCAATGAATTTAACTTAAGTAA  >  NC_000913/4476366‑4476806                                                                                                                                                                                                                                                |                                                                                                                                                                                                          aaaaGGTATTCTTGAAGAAAAAGATAAGCTCTACGCATTAAATGAACACGGAATTGATAATGACAAAGTAGGTAACAATGAAATTAATGACATCAAAGTTAACCTTTCTCATATTCTTATTGATTCCTTAGATGATGCAAAGGTTAACCTTACACCGGTCATCGATTCGATTCTGGAGACTTTTTCAAAATCCCCATATATTAATGATGTAAGAATACTGTATTGGTGTTTTAATAAAAACATGCAATAtt                                                                                                                                                                                                <  1:375395/251‑1 (MQ=255)          aCTTGAAGAAATAGATAAGCTCTACGCATTAAATGATCACGGAATTGATAATGACAAAGTAGGTAACAATGAAATTAATGACATCAAAGTTAACCTGTCTCATATTCTTATTGATTCCTTAGATGATGCAAAGGTTAACCTTACACCGGTCATCGATTCGATTCTGGAGACTTTTTCAAAATCCCCATATATTAATGATGTAAGAATACTGGATTGGTGTTTTAATAAAAACATGCAATATTTTGATGATa                                                                                                                                                                                       <  1:33327/251‑1 (MQ=255)             tGAAGAAATAGATAAGCTCTACGCATTAAATGATCACGGAATTGATAATGACAAAGTAGGTAACAATGAAATTAATGACATCAAAGTTAACCTGTCTCATATTCTTATTGATTCCTTAGATGATGCAAAGGTTAACCTTACACCGGTCATCGATTCGATTCTGGAGACTTTTTCAAAATCCCCATATATTAATGATGTAAGAATACTGGATTGGTGTTTTAATAAAAACATGCAATATTTTGATGATACt                                                                                                                                                                                     <  1:490669/250‑1 (MQ=255)                      aGATAAGCTCTACGCCTTAAATGATCACGGACTTGATAATGACAAAGTAGGTAACAATGAAATTAATGACATCAAAGTTAACCTGTCTCATATTCTTATTGATTCCTTAGATGATGCAAAGGTTAACCTTACACCGGTCATCGATTCGATTCTGGAGACTTTTTCAAAATCCCCATATATTAATGATGTAAGAATACTGGATTGGTGTTTTAATAAAAACATGCAATATTTtgatga                                                                                                                                                                                         >  2:292054/1‑237 (MQ=255)                      aGATAAGCTCTACGCATTAAAAGATCACGGAATTGATAATGACAAAGTAGGTAACAATGAAATTAATGGCATCAAAGTTAACCTGTCTCATATTCTTATTGATTCCTTAGATGATGCAAAGGTTATCCGTTCACCGGTCATCGATTCGATTCTGGAGACTTTTTCAAAATCCCCATATATTAATGATGTAAGAATACTGGATTGGTGTTTTAATAAAAACATGTAATATTTtgatta                                                                                                                                                                                         <  1:292054/237‑3 (MQ=255)                       gATAAGATCTACGCATTAAATGATCACGGAATTGATCATGACAAAGTAGGTAAAAATGAAATTACTGACATCAAAGTTAACCTGTCTCATATTCTTATTGATTCCTTAGATGATGCAAAGGTTAACCTTCCACCGGTCATCGATTCGATTCTGGAGACTTTTTCAAAATCCCCATATATTACAGATGTAAGAATACTGGGTTGGTGTTTTAATAAAAACCTGAAATATTTTGATGAACCTaaaaggttaa                                                                                                                                                                           >  2:6443/1‑244 (MQ=255)                         tAAGCTCTACGCATTAAATGATCACGGAATTGATAATGACAAAGTAGGTAACAATGAAATTAATGACATCAAAGTTAACCTGTCTCATATTCTTATTGATTCCTTAGATGATGCAAAGGTTAACCTTACACCGGTCATCGATTCGATTCTGGAGACTTTTTCAAAATCCCCATATATTAATGATGTAAGAATACTGGATTGGTGTTTTAATAAAAACATGCAATATTTTgatgat                                                                                                                                                                                        >  1:186866/1‑235 (MQ=255)                         tAAGCTCTACGCATTAAATGATCACGGAATTGATAATGACAAAGTAGGTAACAATGAAATTAATGACATCAAAGTTAACCTGTCTCATATTCTTATTGATTCCTTAGATGATGCAAAGGTTAACCTTACACCGGTCATCGATTCGATTCTGGAGACTTTTTCAAAATCCCCATATATTAATGATGTAAGAATACTGGATTGGTGTTTTAATAAAAACATGCAATATTTTgatgat                                                                                                                                                                                        <  2:186866/235‑1 (MQ=255)                                 aCGCATTAAATGATCACGGAATTGATAATGACAAAGTAGGTAACAATGAAATTAATGACATCAAAGTTAACCTGTCTCATATTCTTATTGATTCCTTAGATGATGCAAAGGTTAACCTTACACCGGTCATCGCTTCGATTCTGGAGACTTTTTCAAAATCCCCATATATTAATGATGTAAGAATACTGGATTGGTGTTTTAATAAAATCATGCAATATTTTAATGATACTAAAAAGATAAATCatgcatgc                                                                                                                                                                >  1:93393/1‑251 (MQ=255)                                                                  aaGTAGGTAACAATGAAATTAATGACATCAAAGTTAACCTGTCTCATATTCTTATTGATTCCTTAGATGATGCAAAGGTTAACCTTACACCGGTCATCGATTCGATTCTGGAGACTTTTTCAAAATCCCCATATATTAATGATGTAAGAATACTGGATTGGTGTTTTAATAAAAACATGCAATATTTTGATGATACTAAAAAGATAAAGCATGCATGCTCCGTAATAAATCATATTAATCTTCGCAGCGAt                                                                                                                               >  2:251864/1‑251 (MQ=255)                                                                   aGTAGGTAACAATGAAATTAGTAACATCAAAGTTAACCTGTCTCATATTCTTATTGATTCCTTAGATGATGCAAAGGTTAACCTTACACCGGTCATCGATTCGATTCTGGAGACTTTTTCAAAATCCCCATATATTAATGATGTAAGAATACTGGATTGGTGTTTTAATAAAAACATGCAATATTTTGATGATACTAAAAAGATAAAGCATGCATGCTCCGTAATAAATCATATTAATCTTCGCAGCGATc                                                                                                                              <  1:298649/251‑1 (MQ=255)                                                                              aTGAAATTAATGACATCAAAGTTAACCTGTCTCATATTCGTATTGATTCCTTAGATGATGCAAAGGTTAACCTTACACCGGTCATCGATTCGATTCTGGAGACTTTTTCAAAATCCCCATATATGAATGATGTAAGAATACTGGATTGGTGTTTTAATAAAAACATGCAATATTTTGATGATACTAAAAAGATAAAGCAGGCATGCTCCGTAATAAATCATATGAATCTTCGCATCGATCAGTCTAAAATa                                                                                                                   <  2:46051/251‑1 (MQ=255)                                                                                   cttccatctaTCAAAGTTAACCTGTCTCATATTCTTATTGATTCCTTAGATGATGCAAAGGTTAACCTTACACCGGTCATCGATTCGATTCTGGAGACTTTTTCAAAATCCCCATATATTAATGATGTAAGAATACTGGATTGGTGTTTTAATAAAAACATGCAATATTTTGATGATACTAAAAAGATAAAGCATGCATGCTCCGTAATAAATCATATTAATCTTCGCAGCGATCAGTCTAAAATAGCt                                                                                                                <  1:18138/240‑1 (MQ=255)                                                                                                   ttAACCTGTCTCATATTCTTATTGATTCCTTAGATGATGCAAAGGTTAACCTTACACCGGTCATCGATTCGATTCTGGAGACTTTTTCAACATCCCCATATATTAATGATGTAAGAATACTGGATTGGAGTTTTAATAAAAACATGCAATATTTTGATGATACTAAAAAGATAAAGCATGTATGCTCCGTAATAAATCATATTAATCTTCGCAGCGATCAGTCTAAAATAGCTGAGACATTATTTTTCAAt                                                                                              >  1:54200/1‑251 (MQ=255)                                                                                                              cATATTCTTATTGATTCCTTAGATGATGCAAAGGTTAACCTTACACCGGTCATCGATTCGATTCTGGAGACTTTTTCAAAATCCCCATATATTAATGATGTAAGAATACTGGATTGGTGTTTTAATAAAAACATGCAATATTTTGATGATACTAAAAAGATAAAGCATGCATGCTCCGTAATAAATCATATTAATCTTCGCAGCGATCAGTCTAAAATAGCTGAGACATTATTTTTCAATCTCGATaaa                                                                                     <  1:15711/249‑1 (MQ=255)                                                                                                                          gATTCCTTAGATGATGCAAAGGTTAACCTTACACCGGTCATCGATTCGATTCTGGAGACTTTTTCAAAAGCCCCATATATTAATGATGTAAGAATACTGGATTGGTGTTTTAATAAAAACAAGCAATATTTTGATGATACTAAAAAGATAAAGCATGCATGCTCCTTAATAAATCATATTAATCTTCGCAGTGATCAGTCTAAAATATCTGAGACATTATTTATCAATCTCGATAAAGAACCTTATaaaa                                                                        <  1:438251/250‑1 (MQ=255)                                                                                                                             tCCTTAGATGATGCAAAGGTTAACCTTACACCGGTCATCGATTCGATTCTGGAGACTTTTTCAAAATCCCCATATATTAATGATGTAAGAATACTGGATTGGTGTTTTAATAAAAACATGCAATATTTTGATGATACTAAAAAGATAAAGCATGCATGCTCCGTAATAAATCATATTAATCTTCGCAGCGATCAGTCTAAAATAGCTGAGACATTATTTTTCAATCTCGATAAAGAACCCTATAAAAAt                                                                      >  1:17326/1‑249 (MQ=255)                                                                                                                                               gatAACCTTACACCGGTCATCGATTCGATTCTGGAGACTTTTTCAAAATCCCCATATATGAATGATGTAAGAATACTGGATTGGTGTTTTAATAAAAACATGCAATAGTTTGATGATACTAAAAAGATAAAGCATGCATGCTCCGTAATAAATCATATTAATCTTCGCAGCGACCAGTCTAAAATAGCTGAGACATTATTTTTCAATCTCGATAAAGAGCCCTATAAAAATAGCCCTGAATGACAGGGGtt                                                  <  2:37718/249‑1 (MQ=255)                                                                                                                                                                      ttcgattcTGGAGACTTTTTCAAAATCCCCATATATTAATGATGTAAGAATACTGGATTGGTGTTTTAATAAAAACATGCAATATTTTGATGATACTAAAAAGATAAAGCATGCATGCTCCGTAATAAATCATATTAATCTTCGCAGCGATCAGTCTAAAATAGCTGAGACATTATTTTTCAATCTCGATAAAGAACCCTATAAAAATAGCCCTGAATTACAGGGGTTGATTTGGAATAAGTTGGTTGtat                           >  1:251138/1‑251 (MQ=255)                                                                                                                                                                                      ttttCAAAATCCCCATATATTAATGATGTAAGAATACTGGATTGGTGTTTTAATAAAAACATGCAATATTTTGATGATACTAAAAAGATAAAGCATGCATGCTCCGTAATAAATCATATTAATCTTCGCAGCGATCAGTCTAAAATAGCTGAGACATTATTTTTCAATCTCGATAAAGAACCCTATAAAAATAGCCCTGAATTACAGTGGTTGATTTGGAATAAGTTGGTTGTATATGTCAATGAATTTa            <  1:161392/250‑1 (MQ=255)                                                                                                                                                                                                ccccATATATTAATGATGTAAGAATACTGGATTGGTGTTTTAATAAAAACATGCAATATTTTGATGATACTAAAAAGATAAAGCATGCATGCTCCGTAATAAATCATATTAATCTTCGCAGCGATCAGTCTAAAATAGCTGAGACATTATTTTTCAATCTCGATAAAGAACCCTATAAAAATAGCCCTGAATTACAGGGGTTGATTTGGAATAAGTTGGTTGTATATGTCAATGAATTTAACTTAAGTaa  <  1:372159/250‑1 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                          AAAAGGTATACTTGAAGAAATAGATAAGCTCTACGCATTAAATGATCACGGAATTGATAATGACAAAGTAGGTAACAATGAAATTAATGACATCAAAGTTAACCTGTCTCATATTCTTATTGATTCCTTAGATGATGCAAAGGTTAACCTTACACCGGTCATCGATTCGATTCTGGAGACTTTTTCAAAATCCCCATATATTAATGATGTAAGAATACTGGATTGGTGTTTTAATAAAAGCATGCAATATTTTGATGATACTAAAAAGATAAAGCATGCATGCTCCGTAATAAATCATATTAATCTTCGCAGCGATCAGTCTAAAATAGCTGAGACATTATTTTTCAATCTCGATAAAGAACCCTATAAAAATAGCCCTGAATTACAGGGGTTGATTTGGAATAAGTTGGTTGTATATGTCAATGAATTTAACTTAAGTAA  >  NC_000913/4476366‑4476806

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑