Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,473,900	C→T	G52R (GGA→AGA)	bdcA ←	c‑di‑GMP‑binding biofilm dispersal mediator protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,473,900	0	C	T	100.0%	234.8 / NA	75	G52R (GGA→AGA)	bdcA	c‑di‑GMP‑binding biofilm dispersal mediator protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (36/39);  total (36/39)

TTCACGGAGCCGATGATTAAGATGCGCCCGCCTTCGGGCATCTGCCGGGCGGCTTCAACAGAGGCATGATAAGGAGCATGAATATTGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCT‑CCAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAACAATCTC  >  NC_000913/4473653‑4474145                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                      ttCACGGAGCCGATGATTAAGATGCGCCCGCCTTCGGGCATCTGCCGGGCGGCTTCAACAGAGGCATGATAAGGAGCATGAATATTGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCT‑CTAGt                                                                                                                                                                                                                                                    >  2:486826/1‑251 (MQ=255)   cACGGAGCCGATGATTAAGATGCGCCCGCCTTCGGGCATCTGCCGGGCGGCTTCAACAGAGGCATGATAAGGAGCATGAATATTGATTTTGAAAAGGCGATCAATATCGTCGGCCTTTAATTCCAGGGCCTCGCCAAAGACGCCCATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCCATGACAGCGTCTCTGTCAGCCCTATCTGTAAATACTGCTGTCGCT‑CTAGtct                                                                                                                                                                                                                                                  >  2:294110/1‑251 (MQ=255)   cACGGAGCCGATGATTAAGATGCGCCCGCCTTCGGGCATCTGCCGGGCGGCTTCAACAGAGGCATGATAAGGAGCATGAATATTGATGTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCT‑CTAGtct                                                                                                                                                                                                                                                  <  2:449025/251‑1 (MQ=255)    aCGGAGCCGATGATTAAGATGCGCCCGCCTTCGGGCATCTGCCGGGCGGCTTCAACAGAGGCATGATAAGGAGCATGAATATTGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAAGGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCT‑CTAGtctc                                                                                                                                                                                                                                                 <  1:182238/251‑1 (MQ=255)       gAGCCGATGATTAAGATGCGCCCGCCTTCGGGCATCTGCCGGGCGGCTTCAACAGAGGCATGATAAGGAGCATGAATATTGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCT‑CTAGTCTCTtg                                                                                                                                                                                                                                              >  1:341393/1‑251 (MQ=255)                   aaGATGCGCCCGCCTTCGGGCATCTGCCGGGCGGCTTCAACAGAGGCATGATAAGGAGCATGAATATTGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCT‑CTAGTCTCTTGTGCCCGGCGttt                                                                                                                                                                                                                                  >  1:333093/1‑251 (MQ=255)                               ccTTCGGGCATCTGCCGGGCGGCTTCAACAGAGGCATGATAAGGAGCATGAATATTGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCT‑CTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCttt                                                                                                                                                                                                                      >  1:262542/1‑251 (MQ=255)                               ccTTCGGGCATCTGCCGGGCGGCTTCAACAGAGGCATGATAAGGAGCATGAATATTGATTGTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCT‑CTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCttt                                                                                                                                                                                                                      <  1:376859/251‑1 (MQ=255)                                   cGGGCATCTGCCGGGCGGCTTCAACAGAGTCATGATAAGGAGCATGAATATTGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCT‑CTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGAc                                                                                                                                                                                                                  <  2:262542/251‑1 (MQ=255)                                   cGGGCATCTGCCGGGCGGCTTCAACAGAGGCATGATAAGGAGCATGAATATTGATTTAGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGACGCTCTTACGAACGACATCAATGACAGCGTCTCTGGCAGCACTATCTGTGAATACTGCTGTCGCT‑CTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGa                                                                                                                                                                                                                   <  2:103963/250‑1 (MQ=255)                                    gggCATCTGCCGGGCGGCTTCAACAGAGGCATGATAAGGAGCATGAATATTGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCT‑CTAGTCTCTTGTGCCAGGCGTTTCGCGGCATCTTTCGAcc                                                                                                                                                                                                                 >  2:417054/1‑251 (MQ=255)                                           tGCCGGGCGGCTTCAACAGAGGCATGATAAGGAGCATGAATATTGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCT‑CTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCAt                                                                                                                                                                                                           <  2:284433/250‑1 (MQ=255)                                            gCCGGGCGGCTTCAACAGAGGCATGATAAGGAGCATGAATATTGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCT‑CTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCAt                                                                                                                                                                                                           <  1:249528/249‑1 (MQ=255)                                               gggCGGCTTCAACAGAGGCATGATAAGGAGCATGAATATTGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCT‑CTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTg                                                                                                                                                                                                      >  1:100394/1‑251 (MQ=255)                                                   ggcTTCAACAGAGGCATGTTAAGGAGCATGAATATTGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTACAGGACCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATAACCAATGCGCCGCTCTTACGAACGACATCAATGCCAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGGAGCT‑CTAGTCTCTTGTGCCAGGCGTTTAGCGGCCTCTTTCGGCCCCGCATCGGTGAATc                                                                                                                                                                                                  >  2:506501/1‑251 (MQ=255)                                                          aCAGAGGCATGATAAGGAGCATGAATATTGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCT‑CTAGTCTCTTGTGCCAGGCGTTTAGCGGCAt                                                                                                                                                                                                                          <  2:116948/220‑1 (MQ=255)                                                          aCAGAGGCATGATAAGGAGCATGAATATTGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCT‑CTAGTCTCTTGTGCCAGGCGTTTAGCGGCAt                                                                                                                                                                                                                          >  1:116948/1‑220 (MQ=255)                                                              agGCATGATAAGGAGCATGAATATTGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCT‑CTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGcc                                                                                                                                                                                       >  1:332335/1‑251 (MQ=255)                                                               ggCATGATAAGGAGCATGAATATTGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATCCTGCTGTCGCT‑CTAGTCTCTTTTGCCAGGCGTTTCGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGccc                                                                                                                                                                                      >  2:145798/1‑251 (MQ=255)                                                                gCATGATAAGGAGCATGAATATTGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCT‑CTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGcccc                                                                                                                                                                                     >  1:104840/1‑251 (MQ=255)                                                                 cATGATAAGGAGCATGAATATTGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCT‑CTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCa                                                                                                                                                                                    <  1:79177/251‑1 (MQ=255)                                                                     aTAAGGAGCATGAATATTGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCT‑CTAGTCTCTTGTGCCAGGCGTTTAGCTGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCgg                                                                                                                                                                                >  1:264231/1‑251 (MQ=255)                                                                        aGGAGCATGAATATTGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCT‑CTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTg                                                                                                                                                                              >  2:473984/1‑250 (MQ=255)                                                                         ggAGCATGAATATTGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCT‑CTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGAc                                                                                                                                                                            >  2:275186/1‑251 (MQ=255)                                                                                 aaTATTGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAGTTCCAGAGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCT‑CTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCCTCGGTGACGAAacgac                                                                                                                                                                    >  1:165108/1‑251 (MQ=255)                                                                                   tatTGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCT‑CTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCGTAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGCCGt                                                                                                                                                                  >  1:505710/1‑251 (MQ=255)                                                                                             gAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCT‑CTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCg                                                                                                                                                         <  1:473984/250‑1 (MQ=255)                                                                                                      aTCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCT‑CTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAAt                                                                                                                                                                                                   <  2:501823/199‑1 (MQ=255)                                                                                                      aTCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCT‑CTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAAt                                                                                                                                                                                                   >  1:501823/1‑199 (MQ=255)                                                                                                       tCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCACAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCT‑CTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATAcc                                                                                                                                              >  1:166175/1‑251 (MQ=255)                                                                                                        cAATATCGTCGGCATTTGCTTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCT‑CTAGTCTCTTGTGCCCGGCGATTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGGGCCGAACAGACGGCCGATAGCGGCCCCGATAccc                                                                                                                                             >  2:93682/1‑250 (MQ=255)                                                                                                         aaTATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCT‑CTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCAc                                                                                                                                            >  1:429616/1‑251 (MQ=255)                                                                                                         aaTATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCT‑CTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCAc                                                                                                                                            <  1:387611/251‑1 (MQ=255)                                                                                                                    cATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCT‑CTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGCCGTACGATAGCGGCACCGATACCACGACTGCCACCg                                                                                                                                 >  2:442399/1‑251 (MQ=255)                                                                                                                     aTTTAAATCCAGGGCCTCACCAAAGAAGAGAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAAAGACATAAAAGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCT‑CTAGCCTCTTGTGCCAGGCGTTGAGAGGCATATTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTCACGAAACGCCGCACGATAGCGGCACCGATCACACGACTGcacccg                                                                                                                                 >  2:239104/1‑245 (MQ=255)                                                                                                                         aaTTCCAGGGCCTCGCCAAAGACGCCACTATCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCGCTGTCAGCACTATCTGGGAATACTGCTGTCGCT‑CTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGa                                                                                                                             <  2:114979/250‑1 (MQ=255)                                                                                                                                gggCCTCGACAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCT‑CTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACt                                                                                                                     <  1:489157/251‑1 (MQ=255)                                                                                                                                 ggCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCT‑CTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTg                                                                                                                    <  1:417054/251‑1 (MQ=255)                                                                                                                                    cTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCT‑CTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCt                                                                                                                 >  1:364611/1‑251 (MQ=255)                                                                                                                                      cGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCT‑CTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCtt                                                                                                                <  1:82027/250‑1 (MQ=255)                                                                                                                                         cAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCTCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCGGTGAATACTGCTGTCGCT‑CTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGCCCCCGCATAGTTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGCACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCt                                                                                                            <  2:421460/251‑1 (MQ=255)                                                                                                                                         cAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCT‑CTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCt                                                                                                            <  2:395019/251‑1 (MQ=255)                                                                                                                                                          gCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGATCGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTTTCGCT‑CTAGTCTCTTGTGCCAGGTGTTTAGCGGCATCTTTCTACCCCTCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAg                                                                                           <  1:451707/251‑1 (MQ=255)                                                                                                                                                          gCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCT‑CTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAg                                                                                           <  1:338673/251‑1 (MQ=255)                                                                                                                                                           cATTTACCCCCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTAGCTGTGAATACTGCTGTCGCT‑CTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAgg                                                                                          <  2:68035/251‑1 (MQ=255)                                                                                                                                                           cATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCT‑CTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAgg                                                                                          <  1:486826/251‑1 (MQ=255)                                                                                                                                                                accaccAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCT‑CTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTcc                                                                                     <  1:194266/251‑1 (MQ=255)                                                                                                                                                                 cctccaGGAGATCCAATGCACCCCTCATACGAACTACATCAATGACAGCGTCTCGGTCAGCCCTATCCGTGAATTCTTCTGTCGCT‑CTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGTCCCCATCGGTGACGAAACGACGTACGATATCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCt                                                                                    <  2:421126/248‑1 (MQ=255)                                                                                                                                                                 ccaccaGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCCCTATCTGTGAATACTGCTGTCGCT‑CTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGCGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAggcatcct                                                                                    >  2:217857/1‑245 (MQ=255)                                                                                                                                                                 ccaccaGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCCCTATCTGTGAATACTGCTGTCGCT‑CTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCt                                                                                    <  1:93060/251‑1 (MQ=255)                                                                                                                                                                       ggATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCT‑CTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGc                                                                                                                                                          >  2:147521/1‑175 (MQ=255)                                                                                                                                                                       ggATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCT‑CTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGc                                                                                                                                                          <  1:147521/175‑1 (MQ=255)                                                                                                                                                                          tatCCAATGCGCCGCTCTTACGACCGACATCCATGACAGCGTCTCAGTCAGCACTATCTGTGAATACTGCTGTCGCT‑CTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGGTACGAAACGACGAACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGTCTCCTTGATttatt                                                                           >  1:395984/1‑251 (MQ=255)                                                                                                                                                                                    gccgcTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCT‑CTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATg                                                                 <  1:105541/251‑1 (MQ=255)                                                                                                                                                                                     ccgcTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCT‑CTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGc                                                                >  2:60528/1‑251 (MQ=255)                                                                                                                                                                                                aacgaCACCAATGACAGAGTCTCTGGCAGCACTATCTGTGAATACTGCTGTAGCT‑CTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGAACCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGGACGATAGCGGCACCGATACCCCGCCTGCCACCGAGGCTGAGAACTGTCTTACCTGTAAAAGCGCCCACAAAGACTCCTTGATTTCTTATGGAAAATGCATTACAAAACt                                                     >  2:466502/1‑251 (MQ=255)                                                                                                                                                                                                 acgaCATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCT‑CTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTg                                                    >  1:209698/1‑251 (MQ=255)                                                                                                                                                                                                    gcattaagtgcAGCTTCTCTTTCAGCACTATCTGTGACTTATGCTGTC‑CTGCTAGTGTCTTGGTCCAGGCGTTTACTGGCATCTTTCGACCCCGTATAGGTGAATCGCACATTGGCCCCATCGGTGATGAAACGACGTACGATAGCGGCAACGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCTCCCATAAGGGCTCCTTGATTTATTATGTAACATTCATTACAAAAATGttt                                                 <  2:472684/241‑1 (MQ=255)                                                                                                                                                                                                        cAATGCCAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCATT‑ATAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATCACGGCACCGATCCCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGACCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAAc                                             >  2:460280/1‑251 (MQ=255)                                                                                                                                                                                                         aaTGACAGCGTCTCTGTCAGCACGTTCTGTGAATACTGCTGTCGCT‑TTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGGGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACt                                            <  2:204521/251‑1 (MQ=255)                                                                                                                                                                                                            gACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCT‑CTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACAGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTc                                         >  1:215549/1‑251 (MQ=255)                                                                                                                                                                                                              cAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCT‑CTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCTCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTg                                       <  1:253258/251‑1 (MQ=255)                                                                                                                                                                                                                      ctGTCAGCGCTATCTGGGAATACTGCTGTCTCC‑CTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCTCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCTCCCTTAAGGACTCCTTGATTTATTATGTAACAGGCATTACAAAACTGTTTTAACTTTCTGTCAAc                                  <  2:364611/248‑1 (MQ=255)                                                                                                                                                                                                                        gTCAGCACTATCTGTGAATACTGCTGTCGCT‑CTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAg                                <  1:281439/248‑1 (MQ=255)                                                                                                                                                                                                                        gTCAGCACTATCTGTGAATACTGCTGTCGCT‑CTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCAGTACAAAACTGTTTTAACTTTCTGTCAACAg                                <  1:195760/248‑1 (MQ=255)                                                                                                                                                                                                                        gTCAGCACTATCTGGGAATACTGCTGTCGCG‑CTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGtt                             <  2:474528/251‑1 (MQ=255)                                                                                                                                                                                                                            gCACTATCTGTGAATACTGCTGTCGCT‑CTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGt                         >  2:155976/1‑251 (MQ=255)                                                                                                                                                                                                                             cacgaTCTGTGAATACTGCTGTCGCC‑CTAGTCTCTTGGGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTTAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTa                        <  2:166175/247‑1 (MQ=255)                                                                                                                                                                                                                             cACTATCTGTGAATACTGCTGTCGCT‑CTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGt                         >  1:491252/1‑250 (MQ=255)                                                                                                                                                                                                                              aCTATCTGTGAATACTGCTGTCGCT‑CTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTa                        >  2:20252/1‑250 (MQ=255)                                                                                                                                                                                                                                     gtgAATACTGCTGTCGCT‑CTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGt                   <  1:308568/248‑1 (MQ=255)                                                                                                                                                                                                                                     ctgAATACTGCTGTCGCT‑CTAGCCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACAGTGGCCCCATCGGGGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAATCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTTTTTCATCTTTCTGTCAACAGGTTTTGTAATGGt                   <  1:363330/247‑1 (MQ=255)                                                                                                                                                                                                                                        aaTACTGCTTTCGCT‑CTAGTCTCTTGGGCCAGGCGTTTAGCTGCATCTTTAGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATGTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGTTCACTaa             <  2:373615/251‑1 (MQ=255)                                                                                                                                                                                                                                          tACTGCTGTCGCT‑CTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTaaa            >  1:202808/1‑250 (MQ=255)                                                                                                                                                                                                                                                   cGCT‑CTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAACAAtctc  <  2:209698/251‑1 (MQ=255)                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                      TTCACGGAGCCGATGATTAAGATGCGCCCGCCTTCGGGCATCTGCCGGGCGGCTTCAACAGAGGCATGATAAGGAGCATGAATATTGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCT‑CCAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAACAATCTC  >  NC_000913/4473653‑4474145

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑