| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,463,952 | G→A | S253F (TCC→TTC) | treC ← | trehalose‑6‑phosphate hydrolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,463,952 | 0 | G | A | 87.5% | 127.8 / 5.5 | 56 | S253F (TCC→TTC) | treC | trehalose‑6‑phosphate hydrolase |
| Reads supporting (aligned to +/- strand): ref base G (5/2); new base A (22/27); total (27/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.44e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.22e-01 | |||||||||||
ACCAAAGCGAGAAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATG‑TTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATGAAAATTAAAGGTCATCGACAATTCACTGCCTGT‑CAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGGACATTTCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCAGGCGCAACCCGTCGACCCCACGATCGGCCCAGAACTCACAGACTTTTTTCAGCTCTGCGCGTACCGCTGGATTCTCCCAGTT > NC_000913/4463708‑4464196 | cccAAAGCGATAAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTGCATGCTACGTTGTGCATTCCTTGGTGCCAGTGGCGGAACAATG‑TTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATGAAAATTAAAGGTCATCGACAATTCACTGCCTGT‑CAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGAACAttt < 2:240927/250‑1 (MQ=255) aCAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATG‑TTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATGAAAATTAAAGGTCATCGACAATTCACTGCCTGT‑CAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGAAACTTTCACCTACGGTCAt > 1:125411/1‑251 (MQ=255) cAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATGATTTTCA‑CGACAAAAAATCAGGTTTTGCCAGCGTCCATTTTTCACCACCGGGATAATTGACCTTGAAGGGATGAATAATAAATGTACTCGAAAATTCACAGCCTGT‑CAGAGCCGCGTGTCGCCGGCAATGGTCAAGGCTGGTGGGGGACCtttaaactaccgacatt > 1:59962/1‑237 (MQ=255) aTGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATG‑TTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGCTGAAAATTAAAGGGCATCGACAATTCACTGCCTTT‑CCGAGCCGCGTATCGCTTGCAATGCTCAAGGCTGGTGGAGAACATTTTCCCTACGGTCATTa > 1:250550/1‑250 (MQ=255) gATCGTGGTTACCCCAGAACAATGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATG‑TTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATGAAAATTAAAGGTCATCGACAATTCACTGCCTGT‑CAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGAACATTTCACCTACGGTCATTAACCCGCGTgg < 1:132920/250‑1 (MQ=255) tCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAAAAATG‑TTTTCAAAGCCACAAAGTCAGGGTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCCGGTGATGAAAATTAAAGGTCATCGACAATTCACTGCCTGT‑CAGAGCCGCGGATCGCTGGCAATGCTCAAGGCTGGTGGAGAACATTTTACCCACGGACATTAACCCGCGAGGCGt > 1:287460/1‑251 (MQ=255) aTTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATG‑TTTTCAAAGACCCAACGTCAGGGTTAGCCCGCGTCCCTTTTTCACCCCCGGGATAATTGACCTTCAGGTGGTGAAAATTAAAGGGCATCGACATTTCACTTCCTGTGCA‑AAACGCGTAACGATGGCAATGCTCTAGGCTGGGGGAGGACATTTAACCTTCGGCCATTAAACCGCGTGGGGTagaccccacgcgggtcataccgg > 1:91055/1‑229 (MQ=255) ttCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATG‑TTTTGAAAGCCACAAAGTCAGGGTTAGCCAACGTCCATTTTTCACCACCGGGATAATCGACCTTCCGGGGATGAAAATTAAAAGTCATCGACAATTTACTGCCTGT‑CCAAGCCGAGTATCGCTGGCCAAGCTCAAGGCTGGTGGAGAAAATTTCCCCTACGGTCAATAACCCGCGTGGCGTAAAAACATCGCGGGTCAtcccggg > 1:182685/1‑246 (MQ=255) tCCATGCTACGTTGTGTATTACTTGTTGTCAGTGGCGGAACAATG‑TTTTCAACGCCACAAAGTCAGGTGTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGTTCTTCAGGTGATGAAAACTAAAGGTCATCGACAATTCACTGCCTGT‑CAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGAACATTTCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTg < 1:478222/250‑1 (MQ=255) tCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATG‑TTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATGAAAATTAAAGGTCATCGACAATTCACTGCCTGT‑CAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGAACATTTCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTg < 2:125411/250‑1 (MQ=255) ccATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATG‑TTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGGGATGAAAATTAAAGGTCCTCGACAAATCACTGCCTGT‑CAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGGGGGGAACATTTTACCCACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGc > 2:64384/1‑250 (MQ=255) gtgCATTCCTTGTTGCCAGTGGCGGAACAATG‑TTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATGAAAATTAAAGGTCATCGACAATTCACTGCCTGT‑CAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGAACATTTCCCCTACGGTCATTAACCCGCGGGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACttgaacg > 1:36370/1‑245 (MQ=255) gtgCATTCCTTGTTGCCAGTGGCGGAACAATG‑TTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATGAAAATTAAAGGTCATCGACAATTCACTGCCTGT‑CAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGAACATTTCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACt < 2:36370/245‑1 (MQ=255) ccTTGTTGCCAGTGGCGGAACAATG‑TTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATGAAAATTAAAGGTCATCGACAATTCACTGCCTGT‑CAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGAACATTTCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTg < 1:112400/250‑1 (MQ=255) gCCAGTGGCGGAACAATG‑TTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATGAAAATTAAAGGTCATCGACAATTCACTGCCTGT‑CAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGAACATTTCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCg < 1:13945/251‑1 (MQ=255) ccAGTGGCGGAACAATG‑TTTTCAACGCCACAAAGTCAGGTTTAGCCCGCGTGCATTTTTCACCACCGGGATCATCGGCCTTCAGGTGATGCAAATTACAGGTCATCGACACTTCACTTCCTGT‑CAGAACCGCGTATCGCTGGCACTGCTCCCTGCTGGTGGAGGAAATTTCACCTACGGTCATTAACCCGGGTGGCGTACACACATCGCGGTTCATCTCGTGCCAAAACACTTGTGCTCGTAGCCCGACgg > 2:74154/1‑251 (MQ=255) aCAATG‑TTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATGAAAATTAAAGGTCATCGACAATTCACTGCCTGT‑CAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGAACATTTCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGa < 1:234878/251‑1 (MQ=255) aCAATG‑TTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATGAAAATTAAAGGTCATCGACAATTCACTGCCTGT‑CAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGAACATTTCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGa < 1:348567/251‑1 (MQ=255) tG‑TTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATGAAAATTAAAGGTCATCGACAATTCACTGCCTGT‑CAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGAACATTTCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGcc > 1:177505/1‑251 (MQ=255) gtctgcAACGCCACAAAGTCAGGTTTAGCCGGCGTCCATTTTGCACCACCGGTATAATCGACCTTCAGGTGATGAAAATTAAAGGTCATCGACAATTCACTGCCTGT‑CAGAGCCGCGTATCGCTGGCAATGCGCAAGGCTGGTGGAGAACATTTCACCTACGTTCATTATCCCGCGTGGGGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGccc < 1:479502/246‑1 (MQ=255) aaCGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATGAAAATTAAAGGTCATCGACAATTCACTGCCTGT‑CAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGAACATTTCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGcc < 1:64384/251‑1 (MQ=255) aCGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATGAAAATTAAAGGTCATCGACAATTCACTGCCTGT‑CAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGAACATTTCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCg > 1:273739/1‑251 (MQ=255) gCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATGAAAATTAAAGGTCATCGACAATTCACTGCCTGT‑CAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGGACATTTCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTc > 2:228946/1‑251 (MQ=255) aGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATGAAAATTAAAGGTCATCGACAATTCACTGCCTGT‑CAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGGACATTTCACCTACGGTCATTAACCCGCGTGGCGTaaa < 1:186413/169‑1 (MQ=255) aGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATGAAAATTAAAGGTCATCGACAATTCACTGCCTGT‑CAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGGACATTTCACCTACGGTCATTAACCCGCGTGGCGTaaa > 2:186413/1‑169 (MQ=255) aGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATGAAAATTAAAGGTCATCGACAATTCACTGCCTGT‑CAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGAACATTTCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCAGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTTc > 1:358946/1‑249 (MQ=255) tttttCACCACCGGGATAATCGACCTTCAGGTGATGAAAATTAAAGGTCATCGACAATTCACTGCCTGT‑CAGAGCCGCGTATCGCTGGCCATGCTCAAGGCTGGTGGCGAACATTTCACCTACGGTCATTAACCCGCGTGGCGTACACACCTCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGACCGTCGGTGTAGAAACGACGCCCGGCGCCGTCCGGGTCTTCGGGGAAACCCAGGTCTTTgg > 1:465473/1‑251 (MQ=255) attttCACCACCGGGTGAATCGACCTGCAGGTGATGAAAAGTAAAGGTCATCGACAAGTCACTGCCTGA‑CAGAGCCGCGTATCGCTTGCAATGCTCAAGGCTGGTGGAGAACAGTTCACCTACGTTCATTAACCCGCGTGGCGGAAACACATCGCGGGGCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTTTAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTg < 2:491555/249‑1 (MQ=255) tacaccacCGGGATAATCGACCTTCAGGTGATGAAAATTAAAGGTCATCGACAATTCACTGCCTGT‑CAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGAACATTTCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGgag < 1:8432/248‑1 (MQ=255) caccacCGGGATAAGCGACCTTCAGGTGATGAAAATTAAAGGTCATCGACAATTCACTGCCTGT‑CAGAGCCGCGTATCGCTGGCAATGCTCAAGGCGGGTGGAGAACATTTCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGAt < 1:337822/250‑1 (MQ=255) caccGGGATAATCGACCTTCAGGGGATGAAAATTAAAGGTCATCGACAATTCACTGCCTGT‑CAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGAACATTTCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCCGGGAAACGCGGGGCTTTGGGGATc > 2:455599/1‑248 (MQ=255) gggATAATCGACCTTCAGGTGATGAAAATTAAAGGTCATCGACAATTCACTGCCTGT‑CAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGAACATTTCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTca < 1:83594/251‑1 (MQ=255) aTAATCGACCTTCAGGTGATGAAAATTAAAGGTCATCGACAATTCACTGCCTGT‑CAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGAACATTTCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTGTGGAGATCAGATTcaccc > 1:200890/1‑250 (MQ=255) agcGACCTTCAGGGGATGAAAATTAAAGGTCATCGACAATGCACTGCCTGT‑CAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGAACATTTCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACAt < 2:131810/249‑1 (MQ=255) aTCGACCTTCAGGTGATGAAAATTAAAGGTCATCGACAATTCACTGCCTGT‑CAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGAACATTTCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACAt > 1:122100/1‑251 (MQ=255) cGACCTTCAGGCGATGAAAATTAAAGGTCATCGACAATTCACTGCCTGT‑CAGAGCCGCGTATCGCTGTCAATGCTCAAGGCTGGTGGAGAACATTTCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGCAGAAGCGACGCCCGTCGCCGCCCAGGTCCTCAGGGAAACCCGGGTCTTTGGAGCTAAGATTCACCACATcc > 1:236633/1‑251 (MQ=255) gACCTTCAGGTGATGAAAATTAAAGGTCATCGACAATTCACTGCCTGT‑CAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGAACATTTCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCa < 1:455599/251‑1 (MQ=255) gatgaAAATTAAAGGTCATCGACAATTCACTGCCTGT‑CAGAGCCTCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGAACATTTCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTGTGGAGATCAGATTCACCACATCCAGGCGCAAccc < 2:330803/250‑1 (MQ=255) tgaAAATTAAAGGTCATCGACAATTCACTGCCTGT‑CAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGAACATTTCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCAGGCGCAACCCGTc > 1:447863/1‑251 (MQ=255) aaaGGTCATCGACAATTCACTGCCTGT‑CAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGGACATTTCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCAGGCGCAACCCGTCGACCCCAc < 1:228946/251‑1 (MQ=255) aaaGGTCATCGACAAGTCACTGTCGGT‑CAGAGCGGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGAACATTGCACCTATGGTCATTAACCCGGGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAGCTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGGCGGCCTGCGCTGGCCTGGTCTTCAGGCAAACTCGGGTGGTTGGAGATCAGATTCACCACATCCAGgctctacccttctgccccac < 2:464113/251‑19 (MQ=255) gACATTTCACTGCCTGT‑CAGAGCCGCGTATCGCTGGCAATGCGCAAGGCTGGTGGAGAACATTTCACCTACGGTCATTAACCCGCGGGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCCGATTCACCACATCCAGGCGCAACCCGTCGACCCCACGATCGGCCCa < 2:42371/251‑1 (MQ=255) acaacgcACTGCCTGT‑CAGAGCCGCTTATCGCTGGCAATGCTCAAGTCTGGTGGAGAACATTTCACCTACGGTCATTAACCCGCGTGGCGTAAACCCATCGCGGTTCATCGCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCAGGCGCAACCCGTCGACCCCACGATCGGc < 2:382055/241‑1 (MQ=255) aaTTCACTGCCTGT‑CAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGAACATTTCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCAGGCGCAACCCGTCGACCCCACGATCGGCCCAGa > 1:483670/1‑250 (MQ=255) aTTCACTGCCTGT‑CAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGAACATTTCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCAGGCGCAACCCGTCGACCCCACGATCGGCCCAGAAc < 1:405191/251‑1 (MQ=255) cccTGCCGGT‑CAGGGCCGCGTATCTCTGGCAAGGCGCAAGGCTGGTGGAGAACATTTCACCTACGGTCATTAACCCGCGTGGCGTAACCCCGTCGCGGTTCATCTCGTGCAAAAACGCTTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCGTCAGGGAAACGCGGGTCTTTGGAGATCAGAGTCACCACATCCAGGCGCAACCCGACGACCCCACGATCGGCTCAGAACTca < 2:236633/249‑1 (MQ=255) aCTGCCTGT‑CAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGAACATTTCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCAGGCGCAACCCGTCGACCCCCCGACCGGCCCCGACCTcac > 2:18710/1‑251 (MQ=255) tGCCTGT‑CAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGAACATTTCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGGCTTTGGAGATCAGATTCACCACATCCAGGCGCCACCCGTCGACCCCACGATCGGCCCAGCACTcaca > 2:149810/1‑250 (MQ=255) cAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGAACATTTCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCAGGCGCAACCCGTCGACCCCACGATCGGCCCAGAACTCACAGACtttt < 1:351265/250‑1 (MQ=255) cgcgTATCGCTGGCAATGCTCAAGGCTGGTGGAGAACATTTCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGACTTCAGGGAAACGCGGGTCTTTGGAGATCAGCTTCACCACATCCAGGCGCAACCCGTCGACCCCACGATCGGCCCAGAAATCCCAGACTTTTTTCAGct > 2:505391/1‑251 (MQ=255) gTATCGCTGGCAATGCTCAAGGCTGGTGGAGAACATTTCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCAGGCGCAACCCGTCGACCCCACGATCGGCCCAGAACTCACAGACTTTTTTCAGCTCTg < 1:123596/251‑1 (MQ=255) cTGGCAATGCTCAAGGCTGGTGGAGAACATTTCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCAGGCGCAACCCGTCGACCCCACGATCGGCCCAGAACTCACAGACTTTTTTCAGCTCTGCGCGt > 1:398193/1‑250 (MQ=255) ggCAATGCTCAAGGCTGGTGGAGAACATTTCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCAGGCGCAACCCGTCGACCCCACGATCGGCCCAGAACTCACAGACTTTTTTCAGCTCTGCGCGTAcc < 2:447863/251‑1 (MQ=255) gcacAAGGCGGGTTGAGAACATTGCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAATAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGGCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCAGGCGCAACCCGTCGTCCCCACGATCGGCCCAGAACTCACAGACTTTTTTCAGCTCTGCGCGTACCGCTGGa < 2:442219/248‑1 (MQ=255) tggtggAGAACATTTCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCAGGCGCAACCCGTCGACCCCACGATCGGCCCAGAACTCACAGACTTTTTTCAGCTCTGCGCGTACCGCTGGATTCTccc > 1:325449/1‑249 (MQ=255) gtggAGAACATTTCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCAGGCGCAACCCGTCGACCCCACGATCGGCCCAGAACTCACAGACTTTTTTCAGCTCTGCGCGTACCGCTGGATTCTCCCAGtt > 2:67452/1‑251 (MQ=255) ggagaaCATTTCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCTTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGgag < 1:302612/148‑1 (MQ=255) ggagaaCATTTCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGgag > 2:302612/1‑148 (MQ=255) | ACCAAAGCGAGAAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATG‑TTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATGAAAATTAAAGGTCATCGACAATTCACTGCCTGT‑CAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGGACATTTCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCAGGCGCAACCCGTCGACCCCACGATCGGCCCAGAACTCACAGACTTTTTTCAGCTCTGCGCGTACCGCTGGATTCTCCCAGTT > NC_000913/4463708‑4464196 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |