Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,463,952 G→A S253F (TCC→TTC)  treC ← trehalose‑6‑phosphate hydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,463,9520GA87.5% 127.8 / 5.5 56S253F (TCC→TTC) treCtrehalose‑6‑phosphate hydrolase
Reads supporting (aligned to +/- strand):  ref base G (5/2);  new base A (22/27);  total (27/29)
Fisher's exact test for biased strand distribution p-value = 2.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.22e-01

ACCAAAGCGAGAAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATGAAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGGACATTTCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCAGGCGCAACCCGTCGACCCCACGATCGGCCCAGAACTCACAGACTTTTTTCAGCTCTGCGCGTACCGCTGGATTCTCCCAGTT  >  NC_000913/4463708‑4464196
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                    
cccAAAGCGATAAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTGCATGCTACGTTGTGCATTCCTTGGTGCCAGTGGCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATGAAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGAACAttt                                                                                                                                                                                                                                                <  2:240927/250‑1 (MQ=255)
             aCAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATGAAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGAAACTTTCACCTACGGTCAt                                                                                                                                                                                                                                   >  1:125411/1‑251 (MQ=255)
              cAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATGATTTTCACGACAAAAAATCAGGTTTTGCCAGCGTCCATTTTTCACCACCGGGATAATTGACCTTGAAGGGATGAATAATAAATGTACTCGAAAATTCACAGCCTGTCAGAGCCGCGTGTCGCCGGCAATGGTCAAGGCTGGTGGGGGACCtttaaactaccgacatt                                                                                                                                                                                                                                  >  1:59962/1‑237 (MQ=255)
                aTGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGCTGAAAATTAAAGGGCATCGACAATTCACTGCCTTTCCGAGCCGCGTATCGCTTGCAATGCTCAAGGCTGGTGGAGAACATTTTCCCTACGGTCATTa                                                                                                                                                                                                                                 >  1:250550/1‑250 (MQ=255)
                          gATCGTGGTTACCCCAGAACAATGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATGAAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGAACATTTCACCTACGGTCATTAACCCGCGTgg                                                                                                                                                                                                                       <  1:132920/250‑1 (MQ=255)
                            tCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAAAAATGTTTTCAAAGCCACAAAGTCAGGGTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCCGGTGATGAAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAGCCGCGGATCGCTGGCAATGCTCAAGGCTGGTGGAGAACATTTTACCCACGGACATTAACCCGCGAGGCGt                                                                                                                                                                                                                    >  1:287460/1‑251 (MQ=255)
                                                   aTTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAAAGACCCAACGTCAGGGTTAGCCCGCGTCCCTTTTTCACCCCCGGGATAATTGACCTTCAGGTGGTGAAAATTAAAGGGCATCGACATTTCACTTCCTGTGCAAAACGCGTAACGATGGCAATGCTCTAGGCTGGGGGAGGACATTTAACCTTCGGCCATTAAACCGCGTGGGGTagaccccacgcgggtcataccgg                                                                                                                                                                                             >  1:91055/1‑229 (MQ=255)
                                                    ttCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTGAAAGCCACAAAGTCAGGGTTAGCCAACGTCCATTTTTCACCACCGGGATAATCGACCTTCCGGGGATGAAAATTAAAAGTCATCGACAATTTACTGCCTGTCCAAGCCGAGTATCGCTGGCCAAGCTCAAGGCTGGTGGAGAAAATTTCCCCTACGGTCAATAACCCGCGTGGCGTAAAAACATCGCGGGTCAtcccggg                                                                                                                                                                                            >  1:182685/1‑246 (MQ=255)
                                                     tCCATGCTACGTTGTGTATTACTTGTTGTCAGTGGCGGAACAATGTTTTCAACGCCACAAAGTCAGGTGTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGTTCTTCAGGTGATGAAAACTAAAGGTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGAACATTTCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTg                                                                                                                                                                                            <  1:478222/250‑1 (MQ=255)
                                                     tCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATGAAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGAACATTTCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTg                                                                                                                                                                                            <  2:125411/250‑1 (MQ=255)
                                                      ccATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGGGATGAAAATTAAAGGTCCTCGACAAATCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGGGGGGAACATTTTACCCACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGc                                                                                                                                                                                           >  2:64384/1‑250 (MQ=255)
                                                                  gtgCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATGAAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGAACATTTCCCCTACGGTCATTAACCCGCGGGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACttgaacg                                                                                                                                                                              >  1:36370/1‑245 (MQ=255)
                                                                  gtgCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATGAAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGAACATTTCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACt                                                                                                                                                                                    <  2:36370/245‑1 (MQ=255)
                                                                         ccTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATGAAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGAACATTTCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTg                                                                                                                                                                        <  1:112400/250‑1 (MQ=255)
                                                                                gCCAGTGGCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATGAAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGAACATTTCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCg                                                                                                                                                                <  1:13945/251‑1 (MQ=255)
                                                                                 ccAGTGGCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCCGCGTGCATTTTTCACCACCGGGATCATCGGCCTTCAGGTGATGCAAATTACAGGTCATCGACACTTCACTTCCTGTCAGAACCGCGTATCGCTGGCACTGCTCCCTGCTGGTGGAGGAAATTTCACCTACGGTCATTAACCCGGGTGGCGTACACACATCGCGGTTCATCTCGTGCCAAAACACTTGTGCTCGTAGCCCGACgg                                                                                                                                                               >  2:74154/1‑251 (MQ=255)
                                                                                            aCAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATGAAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGAACATTTCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGa                                                                                                                                                    <  1:234878/251‑1 (MQ=255)
                                                                                            aCAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATGAAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGAACATTTCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGa                                                                                                                                                    <  1:348567/251‑1 (MQ=255)
                                                                                                tGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATGAAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGAACATTTCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGcc                                                                                                                                                >  1:177505/1‑251 (MQ=255)
                                                                                                  gtctgcAACGCCACAAAGTCAGGTTTAGCCGGCGTCCATTTTGCACCACCGGTATAATCGACCTTCAGGTGATGAAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCGCAAGGCTGGTGGAGAACATTTCACCTACGTTCATTATCCCGCGTGGGGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGccc                                                                                                                                               <  1:479502/246‑1 (MQ=255)
                                                                                                        aaCGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATGAAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGAACATTTCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGcc                                                                                                                                         <  1:64384/251‑1 (MQ=255)
                                                                                                         aCGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATGAAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGAACATTTCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCg                                                                                                                                        >  1:273739/1‑251 (MQ=255)
                                                                                                           gCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATGAAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGGACATTTCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTc                                                                                                                                      >  2:228946/1‑251 (MQ=255)
                                                                                                                  aGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATGAAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGGACATTTCACCTACGGTCATTAACCCGCGTGGCGTaaa                                                                                                                                                                                                                 <  1:186413/169‑1 (MQ=255)
                                                                                                                  aGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATGAAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGGACATTTCACCTACGGTCATTAACCCGCGTGGCGTaaa                                                                                                                                                                                                                 >  2:186413/1‑169 (MQ=255)
                                                                                                                      aGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATGAAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGAACATTTCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCAGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTTc                                                                                                                             >  1:358946/1‑249 (MQ=255)
                                                                                                                                        tttttCACCACCGGGATAATCGACCTTCAGGTGATGAAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCCATGCTCAAGGCTGGTGGCGAACATTTCACCTACGGTCATTAACCCGCGTGGCGTACACACCTCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGACCGTCGGTGTAGAAACGACGCCCGGCGCCGTCCGGGTCTTCGGGGAAACCCAGGTCTTTgg                                                                                                         >  1:465473/1‑251 (MQ=255)
                                                                                                                                        attttCACCACCGGGTGAATCGACCTGCAGGTGATGAAAAGTAAAGGTCATCGACAAGTCACTGCCTGACAGAGCCGCGTATCGCTTGCAATGCTCAAGGCTGGTGGAGAACAGTTCACCTACGTTCATTAACCCGCGTGGCGGAAACACATCGCGGGGCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTTTAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTg                                                                                                          <  2:491555/249‑1 (MQ=255)
                                                                                                                                           tacaccacCGGGATAATCGACCTTCAGGTGATGAAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGAACATTTCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGgag                                                                                                       <  1:8432/248‑1 (MQ=255)
                                                                                                                                             caccacCGGGATAAGCGACCTTCAGGTGATGAAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCGGGTGGAGAACATTTCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGAt                                                                                                     <  1:337822/250‑1 (MQ=255)
                                                                                                                                                caccGGGATAATCGACCTTCAGGGGATGAAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGAACATTTCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCCGGGAAACGCGGGGCTTTGGGGATc                                                                                                    >  2:455599/1‑248 (MQ=255)
                                                                                                                                                    gggATAATCGACCTTCAGGTGATGAAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGAACATTTCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTca                                                                                             <  1:83594/251‑1 (MQ=255)
                                                                                                                                                       aTAATCGACCTTCAGGTGATGAAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGAACATTTCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTGTGGAGATCAGATTcaccc                                                                                          >  1:200890/1‑250 (MQ=255)
                                                                                                                                                          agcGACCTTCAGGGGATGAAAATTAAAGGTCATCGACAATGCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGAACATTTCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACAt                                                                                       <  2:131810/249‑1 (MQ=255)
                                                                                                                                                          aTCGACCTTCAGGTGATGAAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGAACATTTCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACAt                                                                                       >  1:122100/1‑251 (MQ=255)
                                                                                                                                                            cGACCTTCAGGCGATGAAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGTCAATGCTCAAGGCTGGTGGAGAACATTTCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGCAGAAGCGACGCCCGTCGCCGCCCAGGTCCTCAGGGAAACCCGGGTCTTTGGAGCTAAGATTCACCACATcc                                                                                     >  1:236633/1‑251 (MQ=255)
                                                                                                                                                             gACCTTCAGGTGATGAAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGAACATTTCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCa                                                                                    <  1:455599/251‑1 (MQ=255)
                                                                                                                                                                        gatgaAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAGCCTCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGAACATTTCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTGTGGAGATCAGATTCACCACATCCAGGCGCAAccc                                                                          <  2:330803/250‑1 (MQ=255)
                                                                                                                                                                          tgaAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGAACATTTCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCAGGCGCAACCCGTc                                                                       >  1:447863/1‑251 (MQ=255)
                                                                                                                                                                                  aaaGGTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGGACATTTCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCAGGCGCAACCCGTCGACCCCAc                                                               <  1:228946/251‑1 (MQ=255)
                                                                                                                                                                                  aaaGGTCATCGACAAGTCACTGTCGGTCAGAGCGGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGAACATTGCACCTATGGTCATTAACCCGGGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAGCTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGGCGGCCTGCGCTGGCCTGGTCTTCAGGCAAACTCGGGTGGTTGGAGATCAGATTCACCACATCCAGgctctacccttctgccccac                                                               <  2:464113/251‑19 (MQ=255)
                                                                                                                                                                                            gACATTTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCGCAAGGCTGGTGGAGAACATTTCACCTACGGTCATTAACCCGCGGGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCCGATTCACCACATCCAGGCGCAACCCGTCGACCCCACGATCGGCCCa                                                     <  2:42371/251‑1 (MQ=255)
                                                                                                                                                                                             acaacgcACTGCCTGTCAGAGCCGCTTATCGCTGGCAATGCTCAAGTCTGGTGGAGAACATTTCACCTACGGTCATTAACCCGCGTGGCGTAAACCCATCGCGGTTCATCGCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCAGGCGCAACCCGTCGACCCCACGATCGGc                                                        <  2:382055/241‑1 (MQ=255)
                                                                                                                                                                                               aaTTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGAACATTTCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCAGGCGCAACCCGTCGACCCCACGATCGGCCCAGa                                                   >  1:483670/1‑250 (MQ=255)
                                                                                                                                                                                                aTTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGAACATTTCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCAGGCGCAACCCGTCGACCCCACGATCGGCCCAGAAc                                                 <  1:405191/251‑1 (MQ=255)
                                                                                                                                                                                                   cccTGCCGGTCAGGGCCGCGTATCTCTGGCAAGGCGCAAGGCTGGTGGAGAACATTTCACCTACGGTCATTAACCCGCGTGGCGTAACCCCGTCGCGGTTCATCTCGTGCAAAAACGCTTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCGTCAGGGAAACGCGGGTCTTTGGAGATCAGAGTCACCACATCCAGGCGCAACCCGACGACCCCACGATCGGCTCAGAACTca                                              <  2:236633/249‑1 (MQ=255)
                                                                                                                                                                                                    aCTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGAACATTTCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCAGGCGCAACCCGTCGACCCCCCGACCGGCCCCGACCTcac                                             >  2:18710/1‑251 (MQ=255)
                                                                                                                                                                                                      tGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGAACATTTCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGGCTTTGGAGATCAGATTCACCACATCCAGGCGCCACCCGTCGACCCCACGATCGGCCCAGCACTcaca                                            >  2:149810/1‑250 (MQ=255)
                                                                                                                                                                                                              cAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGAACATTTCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCAGGCGCAACCCGTCGACCCCACGATCGGCCCAGAACTCACAGACtttt                                     <  1:351265/250‑1 (MQ=255)
                                                                                                                                                                                                                    cgcgTATCGCTGGCAATGCTCAAGGCTGGTGGAGAACATTTCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGACTTCAGGGAAACGCGGGTCTTTGGAGATCAGCTTCACCACATCCAGGCGCAACCCGTCGACCCCACGATCGGCCCAGAAATCCCAGACTTTTTTCAGct                              >  2:505391/1‑251 (MQ=255)
                                                                                                                                                                                                                       gTATCGCTGGCAATGCTCAAGGCTGGTGGAGAACATTTCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCAGGCGCAACCCGTCGACCCCACGATCGGCCCAGAACTCACAGACTTTTTTCAGCTCTg                           <  1:123596/251‑1 (MQ=255)
                                                                                                                                                                                                                             cTGGCAATGCTCAAGGCTGGTGGAGAACATTTCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCAGGCGCAACCCGTCGACCCCACGATCGGCCCAGAACTCACAGACTTTTTTCAGCTCTGCGCGt                      >  1:398193/1‑250 (MQ=255)
                                                                                                                                                                                                                               ggCAATGCTCAAGGCTGGTGGAGAACATTTCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCAGGCGCAACCCGTCGACCCCACGATCGGCCCAGAACTCACAGACTTTTTTCAGCTCTGCGCGTAcc                   <  2:447863/251‑1 (MQ=255)
                                                                                                                                                                                                                                     gcacAAGGCGGGTTGAGAACATTGCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAATAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGGCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCAGGCGCAACCCGTCGTCCCCACGATCGGCCCAGAACTCACAGACTTTTTTCAGCTCTGCGCGTACCGCTGGa             <  2:442219/248‑1 (MQ=255)
                                                                                                                                                                                                                                              tggtggAGAACATTTCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCAGGCGCAACCCGTCGACCCCACGATCGGCCCAGAACTCACAGACTTTTTTCAGCTCTGCGCGTACCGCTGGATTCTccc      >  1:325449/1‑249 (MQ=255)
                                                                                                                                                                                                                                                gtggAGAACATTTCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCAGGCGCAACCCGTCGACCCCACGATCGGCCCAGAACTCACAGACTTTTTTCAGCTCTGCGCGTACCGCTGGATTCTCCCAGtt  >  2:67452/1‑251 (MQ=255)
                                                                                                                                                                                                                                                  ggagaaCATTTCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCTTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGgag                                                                                                       <  1:302612/148‑1 (MQ=255)
                                                                                                                                                                                                                                                  ggagaaCATTTCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGgag                                                                                                       >  2:302612/1‑148 (MQ=255)
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                    
ACCAAAGCGAGAAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATGAAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAGCCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGGACATTTCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCAGGCGCAACCCGTCGACCCCACGATCGGCCCAGAACTCACAGACTTTTTTCAGCTCTGCGCGTACCGCTGGATTCTCCCAGTT  >  NC_000913/4463708‑4464196

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: