Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,439,578	G→A	intergenic (‑316/‑9)	ytfJ ← / → ytfK	PF09695 family protein YtfJ/stringent response modulator YtfK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,439,578	0	G	A	100.0%	152.7 / NA	48	intergenic (‑316/‑9)	ytfJ/ytfK	PF09695 family protein YtfJ/stringent response modulator YtfK
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (26/22);  total (26/22)

TATCGAATTTCCCGCAAGTGTGATGCCAGTTTGCGGTCAAGCGCACAAATCATATGAAAAATGAATGCTTATACTGAAGACCGCGCTTCGGTAAAAAGATAATTCTGAATAATTGTAACCTTTAGGTAAAAAAAGTTATACGCGGTGGAAACATTGCCCGGATAGTCTATAGTCACTAAGCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGAGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTTCCGTGGACGGTTATACATCAAGGACGTTGGCGCTTTTGAATTCGATAAGGGTAAGATTCTTATCCCAAAAGTGAAGGATAAGCTGCATTTGTCAGTGATGTCCGAAGTTAACCGTCAGGTTATGCGTCTGCAAACAGAGATGGCTTAACCAAAGTGCTATGCAGTAAAAAGTGCTAT  >  NC_000913/4439332‑4439822                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                     taacGAATTTCCCGCAAGTGTGATGCCAGTTTGCGGTCAAGCGCACAAATCATATGAAAAATGAATGCTTATACTGAAGACCGCGCTTCGGTAAAAAGATAATTCTGAATAATTGTAACCTTTAGGTAAAAAAAGTTATACGCGGTGGAAACATTGCCCGGATAGTCTATAGTCACTAATCAGTAAAATTTGCGCCTCATAATAGTGGGGCCGATTGTGGCACCGCACAGGCGTTATACGCAGCagaaga                                                                                                                                                                                                                                                   <  2:452696/247‑1 (MQ=255)          tCCCGCAAGTGTGATGCTAGTTTGCGGTCAAGCGCACAAATCATATGAAAAATGAATGCTTATACTGAAGACCGCGCTTCGGTAAAAAGATAATTCTGAATAATTGTAACCTTTAGGTAAAAAAAGTTATACGCGGTGGAAACATTGCCCGGATAGTCTATAGTCACTAAGCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGAAGATAACAATGa                                                                                                                                                                                                                                          <  1:231090/250‑1 (MQ=255)                      gATGCGAGTTTGCGGGCAAGCGCACAAATGGTATGAAAAATGAATGCTTATAATGGAGACCGCGCTACGGTAAAAAGAAAATTATGAATAATTGTAACCTTTAGGTAAAAAAAGTGATACGCGGTGGAAACATTGCCCGGATAGTCTGTAGCCACTAAGCATTAAACTTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACGGCACAGGCGTAATACTCAGCAGAAGATAACAATGCACATTTTCCAACg                                                                                                                                                                                                                             >  2:275988/1‑251 (MQ=255)                                  cGGTCAAGCGCACAAATCATATGAAAAATGAATGCTTATACTGAAGACCGCGCTTCGGTAAAAAGATAATTCTGAATAATTGTAACCTTTAGGTAAAAAAAGTTATACGCGGTGGAAACATTGCCCGGATAGTCTATAGTCACTAAGCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGAAGATAACAATGAAAATTTTCCAACGGTACAACCCACt                                                                                                                                                                                                                 >  2:175262/1‑251 (MQ=255)                                   ggTCAAGCGCACAAATCATATGAAAAATGAATGCTTATACTGAAGACCGCGCTTCGGTAAAAAGATAATTCTGAATAATTGTAACCTTTAGGTAAAAAAAGTTATACGCGGTGGAAACATTGCCCGGATAGTCTATAGTCACTAAGCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGAAGATAACAATGAAAATTTTCCAACGGTACAACCCACtt                                                                                                                                                                                                                >  2:300979/1‑251 (MQ=255)                                               aaaTCATATGAAAAATGAATGCTTATACTGAAGACCGCGCTTCGGTAAAAAGATAATTCTGAATAATTGTAACCTTTAGGTAAAAAAAGTTATACGCGGTGGAAACATTGCCCGGATAGTCTATAGTCACTAAGCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGAAGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGa                                                                                                                                                                                                      >  1:237157/1‑249 (MQ=255)                                                             atgaatgCTTATACTGAAGACCGCGCTTCGGTAAAAAGATAATTCTGAATAATTGTAACCTTTAGGTAAAAAAAGTTATACGCGGTGGAAACATTGCCCGGATAGTCTATAGTCACTAAGCATTAAACTTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGGAATACTCAGCAGAAGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATcat                                                                                                                                                                                      >  2:99723/1‑249 (MQ=255)                                                                   tcTTATACTGATGACCGCTCTTCGGTAAAAAGATAATTCTGAATAATTGTAACCTTTAGGTAAAAAAAGTTATACGCGTTGGAAACATTGCCCGGTTATTCTATAGTCACTAAGCATTAAAATTTGTGCCTCATAAGAGTTTTTCCAAGTGTGTCATCGCACAGGCGTAATACTCAGCAGAAGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCAAAGTTCGTACTGATCCTGtccc                                                                                                                                                                                 <  2:361143/249‑4 (MQ=255)                                                                           tGAAGACCGCGCTTCGGTAAAAAGATAATTCTGAATAATTGTAACCTTTAGGTAAAAAAAGTTATACGCGGTGGAAACATTGCCCGGATAGTCTATAGTCACTAAGCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGAAGATAACAATGAAAATTTTCCAACGGTACAACCCACTACAGGTGGCGAAGTACGTAAAGATCCTGTTCCGTGGACGGt                                                                                                                                                                        >  1:77014/1‑251 (MQ=255)                                                                             aaGACCGCGCTTCGGTAAAAAGATAATTCTGAATAATTGTAACCTTTAGGTAAAAAAAGTTATACGCGGTGGAAACATTGCCCGGATAGTCTATAGTCACTAAGCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGAAGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTTCCGTGGACGGtt                                                                                                                                                                       >  2:84948/1‑250 (MQ=255)                                                                              aGACCGCGCTTCGGTAAAAAGATAATTCTGAATAATTGTAACCTTTAGGTAAAAAAAGTTATACGCGGTGGAAACATTGCCCGGATAGTCTATAGTCACTAAGCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGAAGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTTCCGTGGACGGTtat                                                                                                                                                                     >  2:325857/1‑251 (MQ=255)                                                                                      cTTCGGTAAAAAGATAATTCTGAATAATTGTAACCTTTAGGTAAAAAAAGTTATACGCGGTGGAAACATTGCCCGGATAGTCTATAGTCACTAAGCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGAAGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTTCCGTGGACGGTTATACATCa                                                                                                                                                               >  2:264143/1‑249 (MQ=255)                                                                                         cGGTAAAAAGATAATTCTGAATAATTGTAACCTTTAGGTAAAAAAAGTTATACGCGGTGGAAACATTGCCCGGATAGTCTATAGTCAATAAGCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGAAGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTTCCGTGGACGGTTATCCATCAAGGa                                                                                                                                                           >  1:410553/1‑250 (MQ=255)                                                                                            tAAAAAGATAATTCTGAATAATTGTAACCTTTAGGTAAAAAAAGTTATACGCGGTGGAAACATTGCCCGGATAGTCTATAGTCACTAAGCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGAAGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTTCCGTGGACGGTTATACATCACGGACGtt                                                                                                                                                       >  2:72114/1‑251 (MQ=255)                                                                                               aaaGATAATTCTGAATAATTGTAACCTTTAGGTAAAAAAAGTTATACGCGGTGGAAACATTGCCCGGATAGTCTATAGTCACTAAGCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGAAGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTTCCGTGGACGGTTATACATCAAGGACGTTGgc                                                                                                                                                    <  1:264236/251‑1 (MQ=255)                                                                                                aaGATAATTCTGAATAATTGTAACCTTTAGGTAAAAAAAGTTATACGCGGTGGAAACATTGCCCGGATAGTCTATAGTCACTAAGCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGAAGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTTCCGTGGACGGTTATACATCAAGGACGTTGgcg                                                                                                                                                   <  1:300979/251‑1 (MQ=255)                                                                                                   aTAATTCTGAATAATTGTAACCTTTAGGTAAAAAAAGTTATACGCGGTGGAAACATTGCCCGGATAGTCTATAGTCACTAAGCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGAAGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTTCCGTGGACGGTTATACATCAAGGACGTTGGCGCtt                                                                                                                                                >  1:481847/1‑251 (MQ=255)                                                                                                     aaTTCTGAATAATTGTAACCTTTAGGTAAAAAAAGTTATACGCGGTGGAAACATTGCCCGGATAGTCTATAGTCACTAAGCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGAAGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTTCCGTGGACGGTTATACATCAAGGACGTTGGCGCttt                                                                                                                                               <  1:263595/250‑1 (MQ=255)                                                                                                        tCTGAATAATTGTAACCTTTAGGTAAAAAAAGTTATACGCGGTGGAAACATTGCCCGGATAGTCTATAGTCACTAAGCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGAAGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTTCCGTGGACGGTTATACATCAAGGACGTTGGCGCTTTTGa                                                                                                                                            >  1:186406/1‑250 (MQ=255)                                                                                                         cTGAATAATTGTAACCTTTAGGTAAAAAAAGTTATACGCGGTGGAAACATTGCCCGGATAGTCTATAGTCACTAAGCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGAAGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTTCCGTGGACGGTTATACATCAAGGACGTTGGCGCTTTTGAAt                                                                                                                                          >  1:424617/1‑251 (MQ=255)                                                                                                                atTGTAACCTTTAGGTAAAAAAAGTTATACGCGGTGGAAACATTGCCCGGATAGTCTATAGTCACTAAGCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGAAGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTTCCGTGGACGGTTATACATCAAGGACGTTGGCGCTTTTGAATTCGATa                                                                                                                                    >  2:86147/1‑250 (MQ=255)                                                                                                                   gTAACCTTTAGGTAAAAAAAGTTATACGCGGTGGAAACATTGCCCGGATAGTCTATAGCCACTAAGCATCAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGAAGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTTCCGTGGACGGTTATCCATCAAGGACGTTGGCGCTTTTGAATTCGATAAgg                                                                                                                                 <  1:251115/250‑1 (MQ=255)                                                                                                                          ttAGGTAAAAAAAGTTATACGCGGTGGAAACATTGCCCGGATAGTCTATAGTCACTAAGCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGAAGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTTCGTAAAGATCCTGTTCCGTGGACGGTTATACATCAAGGACGTTGGCGCTTTTGAATTCGATAAGGGTAAGa                                                                                                                           <  2:388338/249‑1 (MQ=255)                                                                                                                            aGGTAAAAAAATTTATACGCGGTGGAAACATTGCCCGGATAGTCTATAGTCACTAATCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGTACCGCACAGGCGTAATACTCAGCAGAAGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGTTCCTGTTCCGTGGACGGTTATACATCAAGGACGTTGGCGCTTTTGAATTCGATAAGGGTAAGATTCt                                                                                                                       <  1:127976/251‑1 (MQ=255)                                                                                                                            aGGTAAAAAAAGTTATACGCGGTGGAAACATTGCCCGGATAGTCTATAGTCACTAAGCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGAAGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTTCCGTGGACGGTTATACATCAAGGACGTTGGCGCTTTTGAATTCGATAAGGGTAAGATTc                                                                                                                        <  1:205135/250‑1 (MQ=255)                                                                                                                                     aaGTTATACGCGGTGGAAACATTGCCCGGATAGTCTATAGTCACTAAGCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGAAGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTg                                                                                                                                                                                                          >  1:362803/1‑159 (MQ=255)                                                                                                                                     aaGTTATACGCGGTGGAAACATTGCCCGGATAGTCTATAGTCACTAAGCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGAAGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTg                                                                                                                                                                                                          <  2:362803/159‑1 (MQ=255)                                                                                                                                                         aTTGCCCGGATAGTCTATAGTCACTAAGCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGAAGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTTCCGTg                                                                                                                                                                              >  2:267056/1‑167 (MQ=255)                                                                                                                                                         aTTGCCCGGATAGTCTATAGTCACTAAGCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGAAGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTTCCGTg                                                                                                                                                                              <  1:267056/167‑1 (MQ=255)                                                                                                                                                             cccGGATAGTCTATAGTCACTAAGCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGAAGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTTCCGTGGACGGTTATACATCAAGGACGTTGGCGCTTTTGAATTCGATAAGGGTAAGATTCTTATCCCAAAAGTGAAGGATAAGCTGCATTTGTc                                                                                      >  1:52088/1‑251 (MQ=255)                                                                                                                                                                ggATAGTCTATAGTCACTAAGCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGAAGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTTCCGTGGACGGTTATACATCAAGGACGTTGGCGCTTTTGAATTCGATAAGGGGAAGATTCTTATCCCAAAAGTGAAGGATAAGCTGCATTTGTCAGt                                                                                   >  2:401251/1‑251 (MQ=255)                                                                                                                                                                    aGTCTATAGTCACTAAGCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGAAGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTTCCGTGGACGGTTATACATCAAGGACGTTGGCGCTTTTGAATTCGATAAGGGTAAGATTCTTATCCCAAAAGTGAAGGATAAGCTGCATTTGTCAGTGAt                                                                                <  1:229531/250‑1 (MQ=255)                                                                                                                                                                           aGTCACTAAGCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGAAGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTTCCGTGGACGGTTATACATCAAGGACGTTGGCGCTTTTGAATTCGATAAGGGTAAGATTCTTATCCCAAAAGGGAAGGATAAGCTGCATTTGTCAGTGATGTCCGAAg                                                                        <  2:481847/251‑1 (MQ=255)                                                                                                                                                                            ggcaatAAGAATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGGGGCACCGCACAGGCGTAATACTCAGCAGAAGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTTCCGTGGACGGTTATACATCAAGGACGGTGGCGCTTTTGAATTCGATAAGGGTAAGATTCTTATCCCAAAAGTGAAGGATAAGCTGCATTTGTCAGTGATGTCCGAAgc                                                                       <  2:186406/246‑2 (MQ=255)                                                                                                                                                                                  aaGCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGAAGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTTCCGTGGACGGTTATACATCAAGGACGTTGGCGCTTTTGAATTCGATAAGGGTAAGATTCTTATCCCAAAAGTGAAGGATAAGCTGCATTTGTCAGTGATGTCCGAAGTTAAc                                                                   <  1:153273/249‑1 (MQ=255)                                                                                                                                                                                  aaGCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGAAGATAACAATGAAAATTTTCCAACGGTACAACACACTTCAGGTGGCGAAGTACGTAAAGATCCTGTTCCGTGGACGGTTATACATCAAGGACGTTGGCGCTTTTGAATTCGATAAGGGTAAGATTCTTATCCCAAAAGTGAAGGATAAGCTGCATTTGTCAGTGATGTCCGAAGTTAACCg                                                                 >  1:227771/1‑251 (MQ=255)                                                                                                                                                                                      aTTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGAAGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTTCCGTGGACGGTTATACATCAAGGACGTTGGCGCTTTTGAATTCGATAAGGGTAAGATTCTTATCCCAAAAGTGAAGGATAAGCTGCATTTGTCAGTGATGTCCGAAGTTAACCGTCAg                                                             <  1:264143/251‑1 (MQ=255)                                                                                                                                                                                       ttAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGAAGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTTCCGTGGACGGTTATACATCAAGGACGTTGGCGCTTTTGAATTCGATAAGGGTAAGATTCTTATCCCAAAAGTGAAGGATAAGCTGCATTTGTCAGTGATGTCCGAAGTTAACCGTCAgg                                                            >  2:87062/1‑251 (MQ=255)                                                                                                                                                                                            aTTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGAGTAATACTCAGCAGAAGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTACCGTTAACGTTTATACATCAAGGTCGTTGGCGCTTTTGAATTCGATAAGGGAATGATTCTTCTACCAAAAGCGAAGGATAAGATGCTTATTTCAGTGATGTCCGAATTTACCCGTAAGGTTATg                                                       >  2:403381/1‑251 (MQ=255)                                                                                                                                                                                              ttGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGAAGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTTCCGTGGACGGTTATACATCAAGGACGTTGGCGCTTTTGAATTCGATAAGGGTAAGATTCTTATCCCAAAAGTGAAGGATAAGCTGCATTTGTCAGTGATGTCCGAAGTTAACCGTCAGGTTATGc                                                      <  1:72114/250‑1 (MQ=255)                                                                                                                                                                                                      cATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGAAGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTTCCGTGGACGGTTATACATCAAGGACGTTGGCGCTTTTGAATTCGATAAGGGTAAGATTCTTATCCCAAAAGTGAAGGATAAGCTGCATTTGTCAGTGATGTCCGAAGTTAACCGTCAGGTTATGCGTCTGCaa                                              >  1:189118/1‑250 (MQ=255)                                                                                                                                                                                                      cATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGAAGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTTCCGTAGACGGTTATACATAAAGGACGTTGGCGCTTTTGAATTCGATAAGGGTAAGATTCTTATCCCAAAAGTGAAGTATACGCTGCATTTGTCAGTGATGTCCGAAGTTAACCGTCAGGATATGCGTCTGCaa                                              >  2:376815/1‑250 (MQ=255)                                                                                                                                                                                                        taataGTTGGGCCGAATGTGGCACCGCACAGGCGTAATACTCAGCAGAAGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTTCCGTGGACGGTTATACATCAAGGACGTTGGCGCTTTTGAATTCGATAAGGGTAAGATTCTTATCCCAAAAGTGAAGGATAAGCTGCATTTGTCAGTGATGTCCGAAGTTAACCGTCAGGTTATGCGTCTGCAAAc                                            >  1:399238/1‑250 (MQ=255)                                                                                                                                                                                                         aataGTTGGGCCGATTGTGGCGCCGCACTGGCGTAATACTCAGCAGAAGATAACAATGAAAATTTTCCAACGGGACAACCCACTTCAGGTGGCGAAGTACGTAAATATCCTGTTCCGTGGACGGTTATGCATCAAGGACGTTGGCGCGTTTGAATTCGAGAAGGTTAAGATTCTTATCCCAAAAGTGAAGGATAAGCTGCAGTTGTCAGTGATGTCCGAAGTTAACCGTCAGGTTATGCGTCTGCAAACa                                           <  1:486564/250‑1 (MQ=255)                                                                                                                                                                                                                        tgtgGCACCGCACAGGCGTAATTCTCAGCAGAAGATAACAATGAAAATTTTCCAACGGTACAACCCCTTTCAGGTGGCGAAGTTCGTAAAGATCCTGTTCCGTGGACGGTTATACATCAAGGACGTGGGCGCTTTTGAATTCGATAAGGGTAAGATTCTTATCCCAAAAGTTAAGGATAAGCTGCATTTGTCAGTGATGGCCGAAGTGAACCGTCAGGTTATGCGTCTGCAAACAGAGATGGCTTAACCa                            <  2:305184/250‑1 (MQ=255)                                                                                                                                                                                                                                    caGGCGTAATACTCAGCAGAAGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTTCCGTGGACGGTTATACATCAAGGACGTTGGCGCTTTTGAATTCGATAAGGGTAAGATTCTTATCCCAAAAGTGAAGGATAAGCTGCATTTGTCAGTGATGTCCGAAGTTAACCGTCAGGTTATGCGTCTGCAAACAGAGATGGCTTAACCaaagtgctatgcag               <  1:273820/251‑1 (MQ=255)                                                                                                                                                                                                                                        cGTACTACACAGCAGAGGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGCGGCGAAGTACGTAAAGATCCTGTTCCGTGGACGGTTCTACATCAAGGACGTTGGCGCATTTGAATTCGATAAGGGTAAGATTCTTATCCCAACAGTGAGGGATAAGCTGCATTTGTCAGTGATGTCCGACGTTAACCGTCAGGTTATGCGACTGCAAACAGAGATGGCTTAAGCaaggtgatatgcagtaac           >  2:87142/1‑250 (MQ=255)                                                                                                                                                                                                                                              aCTCAGCAGAAGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAATTACGTAAAGATCCTGTTCCGTGGACGGTTATACATCAAGGACGTTGGCGCTTTTGAATTCGATAAGGGTAAGATTCTTATCCCAAAAGTGAAGGATAAGCTGCATTTGTCAGTGATGTCCGAAGTTAACCGTCAGGTTATGCGTCTGCAAACAGAGATGGCTTAACCaaagtgctatgcagtcaaaagtg      <  2:148342/250‑1 (MQ=255)                                                                                                                                                                                                                                                 cagcagAAGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTTCCGTGGACGGTTATACATCAAGGACGTTGGCGCTTTTGAATTCGATAAGGGTAAGATTCTTATCCCAAAAGTGAAGGATAAGCTGCATTTGTCAGTGATGTCCGAAGTTAACCGTCAGGTTATGCGTCTGCAAACAGAGATGGCTTAACCaaagtgctatgcagtaaaaagtgctat  <  2:446235/251‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                     TATCGAATTTCCCGCAAGTGTGATGCCAGTTTGCGGTCAAGCGCACAAATCATATGAAAAATGAATGCTTATACTGAAGACCGCGCTTCGGTAAAAAGATAATTCTGAATAATTGTAACCTTTAGGTAAAAAAAGTTATACGCGGTGGAAACATTGCCCGGATAGTCTATAGTCACTAAGCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGAGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTTCCGTGGACGGTTATACATCAAGGACGTTGGCGCTTTTGAATTCGATAAGGGTAAGATTCTTATCCCAAAAGTGAAGGATAAGCTGCATTTGTCAGTGATGTCCGAAGTTAACCGTCAGGTTATGCGTCTGCAAACAGAGATGGCTTAACCAAAGTGCTATGCAGTAAAAAGTGCTAT  >  NC_000913/4439332‑4439822

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑