Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,390,331 C→T Q10Q (CAG→CAA)  psd ← phosphatidylserine decarboxylase proenzyme

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,390,3310CT95.5% 192.3 / 0.4 67Q10Q (CAG→CAA) psdphosphatidylserine decarboxylase proenzyme
Reads supporting (aligned to +/- strand):  ref base C (1/2);  new base T (28/36);  total (29/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.67e-01

AACGCCATCGGCAGGCATGACCAGTACATTCGGATCGGTATCGATTGGGCGTACTTCGTCACGCAGCGGACGGACAAAGAATTCGTTAAAGGTGCGGTAGCTGGCGGTGTCCGGCTTTTGCGCCTCTTTCATGTCGACCTTGTAGTATTTAACGAACAGATCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTCCAGAATACGGTGATAGTTTTCGAAACGGGTTTCCGCGATTTTCCCTTCCTCAACCGCTTCCC  >  NC_000913/4390085‑4390572
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                 
aaCGCCATCGGCAGGCATGACCAGTACATTCGGATCGGTATCGATTGGGCGTACTTCGTCACGCAGCGGACGGACAAAGAATTCGTTAAAGGTGCGGTAGCTGGCGGTGTCCGGCTTTTGCGCCTCTTTCATGTCGACCTTGTAGTATTTAACGAACAGATCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTATTGTa                                                                                                                                                                                                                                               <  1:383051/251‑1 (MQ=255)
 aCGCCAACGGCAGGAATGACCAGTACATTCGGATCGGTATCGATTGGGCGTACTTCGTCACGCAGCGGACGGACAAAGAATTCGTTAAAGGTGCGGTAGCTGGCGGTGTCCGGCTTTTGCGCCTCTTTCATGTCGACCTTGTAGTATTTAACGAACAGATCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTATTGTAg                                                                                                                                                                                                                                              <  2:342741/251‑1 (MQ=255)
      aTCGGCAGGCATGACCAGTACATGCGGAGCGGTAGCGATTGGGCGTACTTCGTCACGCAGCGGACGGACAAAGAATTCGTTAAAGGTGCGGTAGCGGGCGGTGTCCGGCTTTTGCGCCTCTTTCATGTCGACCTTGTAGTATTTAACGAACAGATCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCTCCAACCCGCCAGGCGAGTAAGCCATAGTGTCGGCAGAATGTATTGTAGCGaaa                                                                                                                                                                                                                                         <  2:492401/251‑1 (MQ=255)
            aggcaTGACCAGTACATGCGGAGCGGGATCGATTGGGCGTACTGCGTCACGCAGCGGACGGACAAAGAATTCGTTAAAGGTGCGGTAGCTGGCGGTGTCCGGCTTTTGCGCCTCTTTCATGTCGACCTTGTAGTATTTAACGAACAGATCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCACCCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTATTGTAGCGAAAGTTTaa                                                                                                                                                                                                                                   <  1:70682/251‑1 (MQ=255)
               caTGACCAGTACATTCGGATCGGTATCGATTGGGCGTACTTCGTCACGCAGCGGACGGACAAAGAATTCGTTAAAGGTGCGGTAGCTGGCGGTGTCCGGCTTTTGCGCCTCTTTCATGTCGACCTTGTAGTATTTAACGAACAGATCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTATTGTAGCGAAAGTTTAAATg                                                                                                                                                                                                                                >  1:166893/1‑251 (MQ=255)
               caTGACCAGTACATTCGGATCGGTATCGATTGGGCGTACTTCGTCACGCAGCGGACGGACAAAGAATTCGTTAAAGGTGCGGTAGCTGGCGGTGTCCGGCTTTTGCGCCTCTTTCATGTCGACCTTGGAGTATTTAACGAACAGATCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCTGCAGAATGTATTGTAGCGAAAGTTTAAATg                                                                                                                                                                                                                                <  2:189083/251‑1 (MQ=255)
                               ggATCGGTATCGATGGGGCGTACTTCGTCACGCAGCGGACGGACAAAGAATTCGTTAAAGGTGCGGTAGGTGGCGGTGTCCGGCTTTTGCGCCTCTTTCATGTCGACCTTGTAGTATTTAACGAACAGGTCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCACCCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTATTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGc                                                                                                                                                                                                                <  2:449640/251‑1 (MQ=255)
                                         cGATTGGGCGTACTTCGTCACGCAGCGGACGGACAAAGAATTCGTTAAAGGTGCGGTAGCTGGCGGTGTCCGGCTTTTGCGCCTCTTTCATGTCGACCTTGTAGTATTTAACGAACAGATCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTATTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGcc                                                                                                                                                                                                       >  2:167280/1‑250 (MQ=255)
                                          gATTGGGCGTACTTCGTCACGCAGCGGACGGACAAAGAATTCGTTAAAGGTGCGGTAGCTGGCGGTGTCCGGCTTTTGCGCCTCTTTCATGTCGACCTTGTAGTATTTAACGAACAGATCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTATTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCAt                                                                                                                                                                                                     <  2:166893/251‑1 (MQ=255)
                                                        cGTCACGCAGCGGACGGACAAAGAATTCGTTAAAGGGGCGGTAGCTGGCGGTGTCCGGTTTTTGCGCCTCTTTCATGTCGACCTTGTAGTATTCTACTAACAGACCTATAACCAGTTCTGTCAGCCATCCTGCCCGTTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTATTGTAGCGAAAGTTTAAATGAAGTTTACAAGTGAGCCTCCAGGCCATTGTTTTGTCGTTcc                                                                                                                                                                                       <  1:128923/251‑1 (MQ=255)
                                                         gTCACGCAGCGGACGGACAAAGAATTCGTTAAAGGTGCGGTAGCTGGCGGTGTCCGGCTTTTGCGCCTCTTTCATGTCGACCTTGTAGTATTTAACGAACAGATCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAACGTATTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCt                                                                                                                                                                                      >  1:24300/1‑251 (MQ=255)
                                                               caggggacggacAAAGAATTCGTTAAAGGTGCGGTAGCTGGGGGTGCCCGGCGTTTGCGCCTCTTTCATGTCGACCTTGTAGTATTTAACGAACAGATCGATAACCAGTTTTGGCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAGCAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCg                                                                                                                                                                                <  2:210562/247‑1 (MQ=255)
                                                               cAGCGGACGGACAAAGACTTCGTTAAAGGCGCGGCAGCTGGCGGTGTCCGGCTTTTGCGCCTCTTTCATGTCGACCTTGTAGTATTTAACGAACAGATCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTATTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCg                                                                                                                                                                                >  1:134393/1‑251 (MQ=255)
                                                                 gCGGACGGACAAAGAATTCGTTAAAGGTGCGGTAGCTGGCGGTGTCCGGCTTTTGCGCCTCTTTCATGGCGACCGTGTAGTATTTAACGAACAGATCGATAACCAGTTTTGTCATCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTATTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGc                                                                                                                                                                              <  1:476803/251‑1 (MQ=255)
                                                                    gacggacAAAGAATTCGTTAAAGGTGCGGTAGCTGGCGGTGTCCGGCTTTTGCGCCTCTTTCATGTCGACCTTGTAGTATTTAACGAACAGATCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTATTGTAGCGAAAGTTTAAATGAATTTAACAAGGTa                                                                                                                                                                                                                  >  1:248217/1‑212 (MQ=255)
                                                                    gacggacAAAGAATTCGTTAAAGGTGCGGTAGCTGGCGGTGTCCGGCTTTTGCGCCTCTTTCATGTCGACCTTGTAGTATTTAACGAACAGATCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTATTGTAGCGAAAGTTTAAATGAATTTAACAAGGTa                                                                                                                                                                                                                  <  2:248217/212‑1 (MQ=255)
                                                                           aaaGAATTCGTTAAAGGTGCGGTAGCTGGCGGTGTCCGGCTTTTGCGCCTCTTTCATGTCGACCTTGTAGTATTTAACGAACAGATCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTATTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCg                                                                                                                                                                    <  1:117165/251‑1 (MQ=255)
                                                                            aaGAATTCGTTAAAGGTGCGGTAGCTGGCGGTGTTCGGCTTTTGCGCCTCTTTCATGTCGACCTTGTAGTATTTAACGAACAGATCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTGGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTATTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCgg                                                                                                                                                                   <  1:424133/251‑1 (MQ=255)
                                                                                aTTCGTTAAAGGTGCGGTAGCTTGCGGTTTCCGGCTTTTGCGCCTCTTTCATGTCGACCTTGTAGTATTTAACGAACAGATCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTATTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATcc                                                                                                                                                               <  1:468025/251‑1 (MQ=255)
                                                                                aTTCGTTAAAGGGGCGGTAGCTGGTGGGTTCCGGCTTTTGCGCCTCTTTCATGTCGACCTTGTAGTATTTAACGAACAGATCGATAACCAGTTTTGTCAGCCAGCCTGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTATTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATcc                                                                                                                                                               <  2:6376/251‑1 (MQ=255)
                                                                                        aaGGTGCGGTAGCTGGCGTTGTCCGGCTTTTGCGCCTCTTTCATGTCGACCTTGTAGTATTTAACGAACAGATCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTATTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGaaaaaa                                                                                                                                                       <  1:425380/251‑1 (MQ=255)
                                                                                           gTGCGGTAGCTGGCGGTGTCCGGCTTTTGCGCCTCTTTCATGTCGACCTTGTAGTATTTAACGAACAGATCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGAATTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAgg                                                                                                                                                    >  1:493445/1‑251 (MQ=255)
                                                                                                 tAGCTGGCGGTGTCCGGCTTTTGCGCCTCTTTCATGTCGACCTTGTAGTATTTAACGAACAGATCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTATTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACg                                                                                                                                               >  1:304613/1‑250 (MQ=255)
                                                                                                 tAGCTGGCGGTGTCCGGCTTTTGCGCCTCTTTCATGTCGACCTTGTAGTATTTAACGAACAGATCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTATTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACg                                                                                                                                               >  1:78904/1‑250 (MQ=255)
                                                                                                        cGGTGTCCGGCTTTTGCGCCTCTTTCATGTCGACCTTGTAGTATTTAACGAACAGATCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTATTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAac                                                                                                                                       >  2:463343/1‑251 (MQ=255)
                                                                                                                ggCTTTTGCGCCTCTTTCATGTCGACCTTGTAGTATTTAACGAACAGATCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTATTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTa                                                                                                                               <  2:127584/251‑1 (MQ=255)
                                                                                                                            cctTTCATGTCGGCCTTGTAGTATTTAACGAACAGATCGATAACCATTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTATTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTgtca                                                                                                                   <  2:140129/250‑1 (MQ=255)
                                                                                                                                      cGACCTTGTAGTATTTAACGAACAGATCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTATTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGt                                                                                                          >  1:501117/1‑250 (MQ=255)
                                                                                                                                       gACCTGGTAGTGTTTAACGAAAAGCTAGATAAACAGTTTTGTCAGCCATCCTGCCGGCTTGCTTGCGCACCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTATTGTCGCGGAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGCACCTGAAATAACGGGGACGATTCTGACGCCGGTTCGCTTAGTTgtacgtcttccgtct                                                                                                        >  2:250532/1‑238 (MQ=255)
                                                                                                                                           ttGTAGTATTTAACGAACAGATCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTATTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATc                                                                                                       >  1:162573/1‑248 (MQ=255)
                                                                                                                                            tGTAGTATTTAACGAACAGATCGATAACCAGTTTTGTCAGCCATCCTGCCCGATTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTATTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGACTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACTTGCCGGATCCTGATAAAAAGGGGGAGATTCTAACGACGGTTAGCTTAATTGTCTGTCATCCGAATCAGa                                                                                                    >  2:506402/1‑250 (MQ=255)
                                                                                                                                             gtagtaTTTAACGAACAGATCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTATTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGaa                                                                                                   <  1:401662/250‑1 (MQ=255)
                                                                                                                                                 taTTTAACGAACAGATCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTATTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGtt                                                                                              >  2:98327/1‑251 (MQ=255)
                                                                                                                                                     tAACGAACAGATCGATACCCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTATTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGttttt                                                                                           <  2:501117/250‑1 (MQ=255)
                                                                                                                                                         gcaCAGATCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCACACCCCGCCAGGCGAGTACGCCATAGTTTCGGCAGAATGTATTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGACTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGCCGGTTAGCTTAATTGTCAGTCATCCGGATCAGCAAAGTTTTTAcgcg                                                                                      >  1:41915/3‑251 (MQ=255)
                                                                                                                                                         gAACAGATCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGCATGTATTGTAGCGACAGTTTAAATGACTTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAACAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTAcgcg                                                                                      >  1:41920/1‑251 (MQ=255)
                                                                                                                                                         gAACAGACCGATAACCAGTTTTGGCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAACGTATTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTAcgcg                                                                                      <  2:24300/251‑1 (MQ=255)
                                                                                                                                                          aaCAGATCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTATTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCACTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTaa                                                                                                                         <  1:189374/215‑1 (MQ=255)
                                                                                                                                                          aaCAGATCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTATTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCACTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTaa                                                                                                                         >  2:189374/1‑215 (MQ=255)
                                                                                                                                                                ccGATACCCAGTTTTGTCAGCCCTCCTGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTATTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCGCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTAc                                                                               <  2:134393/250‑1 (MQ=255)
                                                                                                                                                                 cGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTATTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGa                                                                                                                                              <  1:173316/187‑1 (MQ=255)
                                                                                                                                                                 cGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTATTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGa                                                                                                                                              >  2:173316/1‑187 (MQ=255)
                                                                                                                                                                  gATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTATTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACCATTCTAACGACGGTTAGCTTAATTGTCAGTAATCCGTATCAGAAAAGTTTTTACGCGTTTTTActt                                                                             >  2:439529/1‑249 (MQ=255)
                                                                                                                                                                    taagaaGTTTTGTCAGCCATCATGCCCGCTTGCTTGCGCCCCAACCAGCCCGGCGCATAAGCCCTAGTTTCGGCAGAATGTATTGTAGCGAAAGTTTAAATGAATTTAACCAGGTAGCCTCCAGGCCATTGTTTTGTCGTTACTGAATCGGCCTACATGCCGGATCCTGAGAAAAAGGGGGCGCTTCTAACTACGGGTAGCTTAATTGTCATTCATCAGTATCAGAAAATTTATTACACGTTTTTACCTgc                                                                           >  2:404771/6‑251 (MQ=255)
                                                                                                                                                                               gTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTATTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTc                                                                <  1:463343/251‑1 (MQ=255)
                                                                                                                                                                                tCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGTGTAAGCCATAGTTTCGGCAGAATGTATTGTAGCGAACGTTTAAATGATTTTAACAAGGTAGCTTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGTATCCTGTATAAAAGGGTACGATTCTAACGACGGTTAGCTTAATTGTCAGGCATCCGTATCAGAAACGATTTTACGCGTTTTTACCTGCACCATGCttttc                                                               >  2:91129/1‑249 (MQ=255)
                                                                                                                                                                                  aGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGTCCTACATGCCGGATCCTGaaa                                                                                                                                                          >  1:219145/1‑158 (MQ=255)
                                                                                                                                                                                  aGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGTCCTACATGCCGGAGCCTGaaa                                                                                                                                                          <  2:219145/158‑1 (MQ=255)
                                                                                                                                                                                    ccATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAAGGTATTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTCCAGaa                                                           <  2:414660/251‑1 (MQ=255)
                                                                                                                                                                                        ccTGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTATTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTCCAGAATACg                                                       >  1:489258/1‑251 (MQ=255)
                                                                                                                                                                                             ccggtttcattcgcaccAACCCGCCAGGCGAGTAATCCATAGTTTCGGCAGTATGTATTGTAGCGAAAGTTTAAATGAAGTTAACAAGGTAGCGTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCGGAAAAAAAGGGGACGATTCTAACGGCGGGTATCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTGCGCGTTTTTACCTGCGCCATGCTTTCCAGAATACGGTGAt                                                  <  1:250531/236‑1 (MQ=255)
                                                                                                                                                                                             ccGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTATTGTAGCGAAAGTTTAGATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTCCAGAATACGGTGAt                                                  >  2:97789/1‑251 (MQ=255)
                                                                                                                                                                                               gcttgcttGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTATTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTCCAGAATACGGTGAt                                                  >  1:187795/1‑249 (MQ=255)
                                                                                                                                                                                                   gcttgcGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTATTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTCCAGAATACGGTGATAGtttt                                            <  1:403736/251‑1 (MQ=255)
                                                                                                                                                                                                   gcttgcGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTATTGTAGCGAAAGTTGAAATGAATTTAACAAGGTAGCCTCCATGCCATTGTTTTTTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAATTTTTTACGCGTTTTTACCTGCGCCATGCTTTCCAGAATACGGTGATAGttt                                             <  1:343975/250‑1 (MQ=255)
                                                                                                                                                                                                     ttgcGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTATTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTCCAGAATACGGTGATAGTTTTCg                                          >  2:95472/1‑251 (MQ=255)
                                                                                                                                                                                                           cccAACCAGCCAGGCTAGAAAGCCATAGTTGCGGCAGAATGTATTGCAGCGAAGTTTTAAAGGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCCGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTCCAGAATACGGTGATAGTTTTCGAAACgg                                    <  2:9863/251‑1 (MQ=255)
                                                                                                                                                                                                               aCCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTATTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTCCAGAATACGGTGATAGTTTTCGAAACGGGttt                                <  1:460810/251‑1 (MQ=255)
                                                                                                                                                                                                                       ggCGAGTACGCCATAGTTTCGGCAGAATGTATTGTCGCGAACGTTTAATTGAATTTAACCCGGTAGCCTACCGTCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCAGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCCTCCGTATCAGACAATTTTTTACGCGTTTTTACCTGCGCCATGCTTTCACGAATACGGTGCTAGTTTTCGAAACGGGTTTCCCCGAtt                        >  2:492997/1‑251 (MQ=255)
                                                                                                                                                                                                                        gggtgtaaactatatgttCGGCATAATGTATTGTAGCGAAAGTTTAAACGAATTTAACTAGGTAGCCTCCAGGCCATTGTTTTGTCGTTGCTGATTCCGCCTACATGCCGGATCCTGAAAAAAAGGGGTCGATTCTAACGACGGTTACCTTAAGTGTCAGGAATCCGTATCAGAAAAGTTTTTACGCGTTTTTATCTGCGCCATGCTTTCCAGAATACGGTGATAGTTTTCGAAACGGGTTTCCGCGAttt                       <  2:444975/235‑1 (MQ=255)
                                                                                                                                                                                                                         cGAGTAAGCCATAGTTTCGGCAGAATGTATTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTCCAGAATACGGTGATAGTTTTCGAAACGGGTTTCCGCGAtttt                      >  2:222780/1‑251 (MQ=255)
                                                                                                                                                                                                                         cGAGTAAGCCATAGTTTCGGCAGAATGTATTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTCCAGAATACGGTGATAGTTTTCGAAACGGGTTTCCGCGAtttt                      <  1:478529/251‑1 (MQ=255)
                                                                                                                                                                                                                                gCCATAGTTTCGGCAGAATGGATTGTAGCGAAATTTTAAATGAATTTAACACGGGAGCCTCCAGGCCAGTGTGTTGTCGTTCCTGATTCGGCCTACATGCCGGATCCTGAAAAAAAGGTGACGATTCTAACGACTGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTCCAGAATACGGTGATAGTTTTCGAAACGGGTTTCCGCGTTTTTCCCTTcc               <  2:382290/251‑1 (MQ=255)
                                                                                                                                                                                                                                   agattttCGGCAGAATGTATTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTCCAGAATACGGTGATAGTTTTCGAAACGGGTTTCCGCGATTTTCCCTTCCTCa            <  2:162573/247‑1 (MQ=255)
                                                                                                                                                                                                                                             cAGAATGTATTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGTCCTTTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATGCTAACGACGGTTAGCTTAATCGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTCCAGAATACGGTGATAGTTTTCGAAACGGGTTTCCGCGATTTTCCCTTCCTCAACCGCTTccc  <  1:180119/251‑1 (MQ=255)
                                                                                                                                                                                                                                             cAGAATGTATTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTCCAGAATACGGTGATAGTTTTCGAAACGGGTTTCCGCGATTTTCCCTTCCTCAACCGCTTccc  <  1:167280/251‑1 (MQ=255)
                                                                                                                                                                                                                                             cAGAATGTATTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTCCAGAATACGGTGATAGTTTTCGAAACGGGTTTCCGCGATTTTCCCTTCCTCAACCGCTTccc  >  2:205546/1‑251 (MQ=255)
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                 
AACGCCATCGGCAGGCATGACCAGTACATTCGGATCGGTATCGATTGGGCGTACTTCGTCACGCAGCGGACGGACAAAGAATTCGTTAAAGGTGCGGTAGCTGGCGGTGTCCGGCTTTTGCGCCTCTTTCATGTCGACCTTGTAGTATTTAACGAACAGATCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTCCAGAATACGGTGATAGTTTTCGAAACGGGTTTCCGCGATTTTCCCTTCCTCAACCGCTTCCC  >  NC_000913/4390085‑4390572

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑