Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,622	A→T	E274D (GAA→GAT)	thrB →	homoserine kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,622	0	A	T	100.0%	92.5 / NA	34	E274D (GAA→GAT)	thrB	homoserine kinase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (6/28);  total (6/28)

CCAGGATTGCATTGCGCACGGGCGACATCTGGCAGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCAGGCTTCCGGCAGGCGCGGCAGGCGGTCGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGC‑TCTGTGTGACAAGCCGGAAACCGCCCAGC‑GCGTTGCCGACTGGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCACGAGTACTGGAAAACTAAATGAAACTCTACAATCTGAAAGATCACAACGAGCAGGTCAGCTTTGCGCAAGCCGTAACCCAGGGGTTGGGCAAAAATCAGGGGCTGTTTTTTCCGCACGACCTGCCGGAATTCAGCC  >  NC_000913/3391‑3851                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                       ccAGGATTGCATTGCGCACGGGCGACATCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGC‑TCTGTGTGACAAGCCGGATACCGCCCAGC‑GCGTTGCCg                                                                                                                                                                                                                    <  1:192633/251‑1 (MQ=255)         gcattgcGCACGGGCGACATCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGC‑TCTGTGTGACAAGCCGGATACCGCCCAGC‑GCGTTGCCGACTGGtt                                                                                                                                                                                                             <  1:338302/250‑1 (MQ=255)         gcattgcGCACGGGCGACATCTGGCTGGCTTCATTCACGCCTGCTATTCCCGGCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGC‑TCTGTGTGACAAGCCGGATACCGCCCAGC‑GCGTTGCCGACTGGtt                                                                                                                                                                                                             <  2:300369/250‑1 (MQ=255)          cattgcGCACGTGCGACATCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGAGAAGCTGATGAAAGATGTTATCGCCGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGAAATCGGCGCGGTAGCGAGCTGTATCTCCGGCTCCGGCCCGACCTTGTTCGC‑TCTGTGTGACAAGCCGGATACCGCCCAGC‑GCGTTGCCGACTGGTTgg                                                                                                                                                                                                           <  1:21397/251‑1 (MQ=255)                 cACGGGCGACATCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGC‑TCTGTGTGACAAGCCGGGTACCGCCCAGC‑GCGTTGCCGACTGGTTGGGGAAGa                                                                                                                                                                                                     >  1:287285/1‑250 (MQ=255)                      gCGACATCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTACCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGAAATCGGCGCGGTAACGAGCGGTCTCTCCGGCTCCGGCCCGACCTTGTTCGC‑TCTGTGTGGCCAGCCGGATACCGCCCAGC‑GCGTTGCCGACCGGTTGGGTAAGAACtccc                                                                                                                                                                                               >  1:164830/1‑248 (MQ=255)                       cGACATCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGAAATCGGCGCGGTAGCGATCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGC‑TCTGTGTGACAAGCCGGATACCGCCCAGC‑GCGTTGCCGACTGGTTGGGTAAGAACTACCt                                                                                                                                                                                              <  1:213645/251‑1 (MQ=255)                               ggcttgCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGC‑TCTGTGTGACAAGCCGGATACCGCCCAGC‑GCGTTGCCGACTGGTTGGGTAAGAACTACCTGCAAAATc                                                                                                                                                                                      <  1:313586/246‑1 (MQ=255)                               ggctggCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGGTGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGAAAACGGCGCGGGAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTTGC‑TCTGTGGGACAAGACGGATACCGCCCAGC‑GCGTTGCCGACTGGGTGGGTAAGAACTAACTGCACAATc                                                                                                                                                                                      >  1:143275/5‑251 (MQ=255)                                       tcattcaCGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGAAAGCGGCGCGGTAGCGAGCGTTATCTCCGGCTCCGGCCCGACCTTGTTCGC‑TCTGTGTGACAAGCCGGATACCGCCCAGC‑GCGTTGCCGACTGGTTGGGTAAGAACTACCTGCAAAATCAGGAAgg                                                                                                                                                                               <  1:14480/250‑1 (MQ=255)                                       tcattcaCGCCGGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGC‑TCTGTGTGACAAGCCGGATACCGCCCAGC‑GCGTTGCCGACTGGTTGGGTAAGAACTACCTGCAAAATCAGGAAgg                                                                                                                                                                               <  1:85660/250‑1 (MQ=255)                                        cattcaCGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGC‑TCTGTGTGACAAGCCGGATACCGCCCAGC‑GCGTTGCCGACTGGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGt                                                                                                                                                                              <  1:387818/250‑1 (MQ=255)                                            caCGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCGTCCGGCAGGCGCGGCAGGCGGTTGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGC‑TCTGTGTGACAAGCCGGATACCGCCCAGC‑GCGTTGCCGACTGGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGt                                                                                                                                                                         <  2:144697/251‑1 (MQ=255)                                                          ccGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGAAATCGGCGCGGTAGCGAGCGGGATCTCCGGCTCCGGCCCGACCTTGTTCCC‑TCTGTGTGACAAGCCGGGTACCGCCCAGC‑GCGTTGCCGGCTGGTTGGGGTAGAAATAACTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCggg                                                                                                                                                           >  1:120096/1‑250 (MQ=255)                                                                 ccTGAGCTTGCCGCGAAGCTTATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGTGGCAGGCGGTTGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTTTTCGC‑TCTGTGTGACAAGCCGGATACCGCCCAGC‑GCGTTGCCGACTGTTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATAc                                                                                                                                                    <  1:318139/251‑1 (MQ=255)                                                                     aGCTTGCCGCGAAGCGGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGC‑TCTGTGTGACAAGCCGGATACCGCCCAGC‑GCGTTGCCGACTGGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATAcggcg                                                                                                                                                <  2:304675/251‑1 (MQ=255)                                                                         tGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGC‑TCTGTGTGACAAGCCGGATACCGCCCAGC‑GCGTTGCCGACTGGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACggcggg                                                                                                                                              <  1:362061/249‑1 (MQ=255)                                                                               gAAGCTGATGAAAGATGTTATCGCTGAGCCCTACCGGGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGC‑TCTGTGTGACAAGCCGGATACCGCCCAGC‑GCGTTGCCGACTGGTTGGGTAAGAACTACCTGCAAAAGCAGGAAGGTTTGGTTCATATTTGCCGGCTGGATACGGCGGGCGCACGAg                                                                                                                                      <  1:404641/251‑1 (MQ=255)                                                                                     gatgaAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGC‑TCTGTGTGACAAGCCGGATACCGCCCAGC‑GCGTTGCCGACTGGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCACGAGTACTg                                                                                                                                 <  1:129041/250‑1 (MQ=255)                                                                                        gaAAGATGTTATCGCTGAACCCGACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGC‑TCTGTGTGACAAGCCGGATACCGCCCAGC‑GCGTTGCCGACTGGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCACGAGTACTGGaaa                                                                                                                             <  2:392669/251‑1 (MQ=255)                                                                                         aacgATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGAAATCGGCGCGGTCGCGAGCGGTATCTCCGGCTCCCGCCCGACCTTGTTCGC‑TCTGTGTGACAAGCCGGATAACGCCCCGC‑GCCTTGCCCACTAGTTGGGGAAGAAATACCTGCAAAATCAGGGAGGTTTTGTTCATATTTTCCGGCTGGATAAggcgggggggtacaggagatg                                                                                                                               >  2:397058/4‑233 (MQ=255)                                                                                             aTGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGC‑TCTGTGTGACAAGCCGGATACCGCCCAGC‑GCGTTGCCGACTGGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACggcggggggccacggactgggaaaata                                                                                                                         >  1:394455/1‑229 (MQ=255)                                                                                                    cGCTGAACCCTACCGTGAACGTTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGC‑TCTGTGTGACAAGCCGGATACCGCCCAGC‑GCGTTGCCGACTGGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCACGAGTACTGGAAATCTAAATGAAAc                                                                                                                 <  2:483215/251‑1 (MQ=255)                                                                                                     gCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGC‑GCTGTGTGACAAGCCGGATACCGCCCAGC‑GCGTTGCCGACTGGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTGTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCACGAGTACTGGAAAACTAAATGAAAct                                                                                                                <  1:58659/251‑1 (MQ=255)                                                                                                                                   ggCTGCCGGCAGGCGCGGCAGGCGGTTGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGC‑TCTGTGTGACAAGCCGGATACCGCCCAGC‑GCGTTGCCGACTGGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCACGAGTACTGGAAAACTAAATGAAACTCTACAATCTGAAAGATCACAATGAGCAgg                                                                                   <  1:194454/250‑1 (MQ=255)                                                                                                                                         cggcaggcgcggcaggcgGTTGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGC‑TCTGTGTGACAAGCCGGATACCGCCCAGC‑GCGTTGCCGACTGGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCACGAGTACTGGAAAACTAAATGAAACTCTACAATCTGAAAGATCACAATGAGCAGGTCAGCtt                                                                            <  1:479116/251‑1 (MQ=255)                                                                                                                                                       ggcgGTTGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGC‑TCTGTGTGACAAGCCGGATACCGCCCAGC‑GCGTTGCCGACTGGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCACGAGTACTGGAAAACTAAATGAAACTCTACAATCTGAAAGATCACAAGGAGCAGGTCAGCTTTGCGCAAGCCGTaa                                                              <  1:170277/251‑1 (MQ=255)                                                                                                                                                                   aaccggctcgggggcgtgcggttgctccggctcgggCCCGAC‑TTGGTCGCCTCTGGTTGACAAGCCCGATGCCGACC‑GCTGCGTTGCCGACTGGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCCCGAGTACTGGAAAACTAAATGAAACTCTACAATCTGAAGGATCACAAGGAGCAGGTGAGTTTTGCGCAAGCCGTAACCAAGGGGTTgg                                                  <  2:118650/227‑1 (MQ=255)                                                                                                                                                                           cgGTAGCGAGCGGTAGCTCCGGCTCCGGCCCGACCTTGTTCGC‑TCTGTGTGACAAGCCGGATACCGCCCAGC‑GCGTTGCCGACTGGTTGGTTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCACGAGTACTGGAAAACTAAATGAAACTCTACAATCTGAAAGATCACAATGAGCAGGTCAGCTTTGCGCAAGCCGTAACCCAGGGGTTGGGCAAAAAt                                          <  1:284576/251‑1 (MQ=255)                                                                                                                                                                                  gagcgGTATCTCCGGCTCCGGCCCGACCTTGTTCGC‑TCTGTGTGACAAGCCGGATACCGCCCAGC‑GCGTTGCCGACTGGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCACGAGTACTGGAAAACTAAATGAAACTCTACAATCTGAAAGa                                                                                                 <  1:339542/189‑1 (MQ=255)                                                                                                                                                                                                   ccgtcccGGCCTTGTTCGT‑TGTGAGTAACAAGCCGGATACCGCCCAGG‑GCGTTGCCGACGGGTTGGGTAAGAACTACCTGCAAAAGCAGGAAGGTTTTGTCCATATTTGCCGGCTGGATACGGCGGGCGCACGAGTACTGGAAAACAAAAGGAAACTCTACAATCTGAAAGATCACAATGAGCAGGTCAGCTTTGCGCAAGCCGTAACCCAGGGGTTGGGCAAAAATCAGGGCCTGTTTTTTCCCCACGAc                  <  2:307419/247‑1 (MQ=255)                                                                                                                                                                                                       cccGTCCTTGGTCGC‑GCTGGGTGTCAAGCCGGATACCGCGCAGC‑GCGTTGCCGACTGGTGGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCGGGATACGGCGGGCGCACGAGTACTGGAAAACTAAATGAAACTCTACAATCTGAAAGAGCACAATGAGCAGGTCAGCTTTGCGCAAGCCGTAACCCAGGGGTTGGGCAAAAATCAGGTGCTGTTTTTTCCGCACCACCTGc              <  2:113851/251‑1 (MQ=255)                                                                                                                                                                                                                   cGC‑TCTGTGTGACAAGCCGGATACCGCCCAGC‑GCGTTGCCGACTGGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCACGAGTTCTGGAAAACTAAATGAAACTCTACAATCTGAAAGATCACAATGAGCAGGTCAGCTTTGCGCAAGCCGTAACCCAGGGGTTGGGCAAAAATCAGGGGCTGTTTTTTCCGCACGACCTGCCGGAATTCAGcc  <  1:397058/251‑1 (MQ=255)                                                                                                                                                                                                                   cGC‑TCTGTGTGACAAGCCGGATACCGCCCAGC‑GCGTTGCCGACTGGTGGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCCCGAGTACTGGAAAACTAAATGAAACTCTACAATCTGAAAGATCACAATGAGCAGGTCAGCTTTGCGCAAGCCGTAACCCAGGGGTTGGGCAAAAATCAGGGGCTGTTTTTTCCGCACGACCTGCCGGAATTCAGc   <  1:78141/250‑1 (MQ=255)                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                       CCAGGATTGCATTGCGCACGGGCGACATCTGGCAGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCAGGCTTCCGGCAGGCGCGGCAGGCGGTCGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGC‑TCTGTGTGACAAGCCGGAAACCGCCCAGC‑GCGTTGCCGACTGGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCACGAGTACTGGAAAACTAAATGAAACTCTACAATCTGAAAGATCACAACGAGCAGGTCAGCTTTGCGCAAGCCGTAACCCAGGGGTTGGGCAAAAATCAGGGGCTGTTTTTTCCGCACGACCTGCCGGAATTCAGCC  >  NC_000913/3391‑3851

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑