Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,331,890	C→T	L463L (CTC→CTT)	proP →	osmolyte:H(+) symporter ProP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,331,890	0	C	T	100.0%	103.9 / NA	33	L463L (CTC→CTT)	proP	osmolyte:H(+) symporter ProP
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (14/19);  total (14/19)

ATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAGAATCTGATGATGCCTGCCTATTACCTGATGGTAGTGGCGGTGGTTGGTTTAATCACCGGCGTAACCATGAAAGAGACGGCAAATCGTCCGTTGAAAGGTGCGACACCGGCGGCGTCAGATATACAGGAAGCGAAGGAAATTCTCGTCGAGCATTACGATAATATCGAGCAGAAAATCGATGATATTGACCACGAGATTGCCGATTTGCAGGCGAAACGTACCCGCCTGGTGCAGCAACATCCGCGAATTGATGAATAAGCTGAAACGGATGGCCTGATGTGACGCTGTCTTATCAGGCCAATTGAACTCTTAAGGTTCACTTAATCTCTGACGCGCATACTCTCCTCCAGGTTAACGGAGGAGAGTGCAATGAAAAACCGTGTTTATG  >  NC_000913/4331651‑4332134                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                     atCCGTTGCAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAGAATCTGATGATGCCTGCCTATTACCTGATGGTAGTGGCGGTGGTTGGTTTAATCACCGGCGTAACCATGAAAGAGACGGCAAATCGTCCGTTGAAAGGTGCGACACCGGCGGCGTCAGATATACAGGAAGCGAAGGAAATTCTTGTCGAGCAtt                                                                                                                                                                                                                                            <  2:159380/250‑1 (MQ=255)    cGTTACAGCGCGCTGGCGGCGTCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAGAATCTGATGATGCCTGCCTATTACCTGATGGTAGTGGCGGTGGTTGGTTTAATCACCGGCGTAACCATGAAAGAGACGGCAAATCGTCCGTTGAAAGGTGCGACACCGGCGGCGTCAGATATACAGGAAGCGAAGGAAATTCTTGTCGAGCATTACGa                                                                                                                                                                                                                                        <  1:251320/251‑1 (MQ=255)       tACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAGAATCTGATGATGCCTGCCTATTACCTGATGGTAGTGGCGGTGGTTGGTTTAATCACCGGCGTAACCATGAAAGAGACGGCAAATCGTCCGTTGAAAGGTGCGACACCGGCGGCGTCAGATATACAGGAAGCGAAGGAAATTCTTGTCGAGCATTACGata                                                                                                                                                                                                                                      <  1:202102/250‑1 (MQ=255)               gcgggcggctgcATTTAATATTTCGGTGCTGGGTGCCGGTTTGACGCCAACGCTGGCGGCTTGGCGTGTCGAAAGCTAGCTGAATCTGATGATGCCTGCCTATTACCTGATGGTAGTGGCGGTGGTTGGTTTAATCACCGGCGTAACCATGAAAGAGACGGCAAATCGTCCGTTGAAAGGTGCGACACCGGCGGCGTCAGATATACAGGAAGCGAAGGAAATTCTTGTCGAGCATTACGATAATATCGAGc                                                                                                                                                                                                                             <  2:118603/248‑1 (MQ=255)                     ggcggcATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCAAACGCTGGCGGCCTGGCTGGGCGAAAGCTCGCAGAATCTGATGATGCCTGCCTATTACCTGATGGTAGTGGCGGTGGTTGGTTTAATCACCGGCGTAACCATGAAAGAGACGGCAAATCGTCCGTTGAAAGGTGCGACACCGGCGGCGTCAGATATACAGGAAGCGAAGGAAATTCTTGTCGAGCATTACGATAATATCGAGCAGaaaa                                                                                                                                                                                                                       <  1:341795/251‑1 (MQ=255)                                                tGCCGGTACGACGCCAACGCTGGCGGTCTGGCTGGTCGAAAGCTCGCAGAATCTGATGATGCCTGCCTATTACCTGATGGTAGTGGCGGTTGTTGGTTTAATCACCGGCGTAACCATGAAAGAGACGGCAAAGCGTCCGTTGAAAGGTGCGACACCGGCGGCGTCAGATATACAGGAAGCGAAGGAAATGCTTGTCGAGCATTACGATAATATCGAGCAGAAAAGCGATGATATTGACCACGAGATTGcc                                                                                                                                                                                             <  2:173376/250‑1 (MQ=255)                                                 gcctgTCTGACCCCAACGCTGGCGGCCTGGCTGTTCGAAAGCTCGCAGAATCTGTTGATGCCGTCCTATTACCTGATGGTAGTGTCGGTGGTTGGTTTAATCACCGGCGTAACCATGAAAGAGACGGCAAATCGTCCGTTGAAAGGTGCGACACCGGCGGCGTCAGATATACAGGAAGCGAAGGAAATTCTTGCCGAGCATTACGATAATATCGAGCAGAAAATCGAGGATATTGTCCACGAGATTTCCg                                                                                                                                                                                            <  2:376228/246‑1 (MQ=255)                                                                     ggcggcCTGGCTGGTCGAAAGCTCGCAGAATCTGATGATGCCTGCCTATTACCTGATGGTAGTGGCGGTGGTTGGTTTAATCACCGGCGTAACCATGAAAGAGACGGCAAATCGTCCGTTGAAAGGTGCGACACCGGCGGCGTCAGATATACAGGAAGCGAAGGAAATTCTTGTCGAGCATTACGATAATATCGAGCAGAAAATCGATGATATTGACCACGAGATTGCCGATTTGCAGGCGAAACGTAcc                                                                                                                                                                        <  2:130269/250‑1 (MQ=255)                                                                                                      gttgatgCCTGCCTAATACCTGATGGTAGTGGCGGTGTTGGGTTTAATCACCGGCTTAACCATGAAAGAGACGGCAAATCGTCCGTTGAAAGGTGCGACCCCGGCGGCGTCAGATATACAGGAAGCGAAGGAAATTCTTGTCGAGCATTACGATAATATCGAGCAGAAAACCGATGATATTGACCTCGTGATTGCCGATTTGCAGGCGAAACGTACCCGCCTGGTGCAGCAACATCCGCGAATTGATGaa                                                                                                                                       <  1:504206/248‑1 (MQ=255)                                                                                                                               gTAGTGGCGGTGGTTGGTTTAATCACCGGCGTAACCATGAAAGAGACGGCAAATCGTCCGTTGAAAGGTGCGACACCGGCGGCGTCAGATATACAGGAAGCGAAGGAAATTCTTGTCGAGCATTACGATAATATCGAGCAGAAAATCGATGATATTGACCACGAGATTGCCGATTTGCAGGCGAAACGTACCCGCCTGGTGCAGCAACATCCGCGAATTGATGAATAAGCTGAAACGGATGGCCTGAtgtg                                                                                                             <  1:175529/251‑1 (MQ=255)                                                                                                                                   tGGCGGTGGGTGGTTTAATCACCGGCGTAACAATGAAAGAGACGGCAAATCGTCCGTTGAAAGGTGCGACACCGGCGGCGTCAGATATACAGGAAGCGAAGGAAATTCTTGTCGAGCATTACGATAATATCGAACAGAAAATCGATGATATTGACCACGAGATTGCCGATTTGCAGGCGAAACGTACCCGCCTGGGGCAGCACAATCCGCGAATTGATGAATAAGCTGCAACAGATGGCCTGATGTGACg                                                                                                          >  1:390038/1‑250 (MQ=255)                                                                                                                                    gggggtgggtgGTGTAATCACGGGCGTACCCATGAAAGGGACGGAAAATCGTCCTTTGAAAGGTGCGTCACGGGGGGGGCCAGATAAAAGGGAAGCGAAGGAAATTCTTGTCGAGCATTACGATAATACCGGGAAGAAAATCGATGAGATTTACCTCGAGATTGCGGGTTTGCAGGGGAAACGTCCCCGCCTGGTGCAGCAACATCCGCGAATTGATGAATAAGCTGAAACGGATGGCCTGATGTGACGCt                                                                                                        <  2:483176/248‑1 (MQ=255)                                                                                                                                    ggaggtggtTGGTTTAATCACCGGGGTAACAATGAAAGACACGGAAAATCGTCCGTTGAAAGGTGCGACGCGGGCGGCGACAGATATACAGGAAGCGAAGGAAATTCTTGTCGAGCCTTACGTTAATATCGAGCAGAAAATCGATGATATTGACCACGAGATGGCCAATTTGCAGGCGAAACGTACCCGCCTTGTGCAGCAACATCCGCGAATTGATGAATAAGCTGAAACGGATGGCCTGATGTGACGc                                                                                                         <  1:325998/247‑1 (MQ=255)                                                                                                                                           gttggttTAATCACAGGCGGAACCATGAAAGAGACGGCAAATCGTCCGTTGAAAGGTGCGACACCGGCGGCGTCAGATATACAGGAAGCGAAGGAAATTCTTGTCGAGCATTACGATAATATCGAGCAGAAAATCGATGATATTGACCACGAGATTGCCGATTTGCAGGCGAAACGTACCCGCCTGGTGCAGCCACATCCGCGAATTGATGAATAAGCTGAAACGGATGGCCTGATGTGACGCTGTCTTAt                                                                                                 >  1:379784/1‑251 (MQ=255)                                                                                                                                                  taaacACCGACGGAACCATGAAATAGACGGAAAAGCGTCCGTTGAAAGGTGCGACACCTGCGGCGTCAGATATACAGGAAGCGAAGAAAATTCTTGTCGAGCATTCCGATAATATCGAGCAGAAAATCGAGGATTTGGACCACGAGATTGCCGATTTGCAGGAGAAACGTACCCGCCTGGTGCAGCAACATCCGCGAATTGATGAATAAGCTGAAACGGATGGCCTGATGTGACGCTGTCTTATCAGGcc                                                                                           <  2:31686/246‑1 (MQ=255)                                                                                                                                                         cGGCGTAACCATGAAAGAGACGGCAAATCGTCCGTTGAAAGGTGCGACACCGGCGGCGTCAGATATACAGGAAGCGAAGGAAATTCTTGTCGAGCATTACGATAATATCGAGCAGAAAATCGATGATATTGACCAGGAGATTGCCGATTTGCAGGCGAAACGTACCCg                                                                                                                                                                      <  2:90358/168‑1 (MQ=255)                                                                                                                                                         cGGCGTAACCATGAAAGAGACGGCAAATCGTCCGTTGAAAGGTGCGACACCGGCGGCGTCAGATATACAGGAAGCGAAGGAAATTCTTGTCGAGCATTACGATAATATCGAGCAGAAAATCGATGATATTGACCACGAGATTGCCGATTTGCAGGCGAAACGTACCCg                                                                                                                                                                      >  1:90358/1‑168 (MQ=255)                                                                                                                                                          ggCGTAACCATGAAAGAGACGGCAAATCGTCCGTTGAAAGGTGCGACACCGGCGGCGTCAGATATACAGGAAGCGAAGGAAATTCTTGTCGAGCATTACGATAATATCGAGCAGAAAATCGATGATATTGACCACGAGATTGCCGATTTGCAGGCGAAACGTACCCGCCTGGTGCAGCAACATCCGCGAATTGATGAATAAGCTGAAACGGATGGCCTGATGTGACGCTGTCTTATCCGGCCAATTGaaat                                                                                  >  1:481249/1‑249 (MQ=255)                                                                                                                                                          ggCGTAACCATGAAAGAGACGGCAAATCGTCCGTTGAAAGGTGCGACACCGGCGGCGTCAGATATACAGGAAGCGAAGGAAATTCTTGTCGAGCATTACGATAATATCGAGCAGAAAATCGATGATATTGACCACGAGATTGCCGATTTGCAGGCGAAACGTACCCGCCTGGTGCAGCAACATCCGCGAATTGATGAATAAGCTGAAACGGATGGCCTGATGTGACGCTGTCTTATCAGGCCAATTGAAct                                                                                  >  1:410983/1‑251 (MQ=255)                                                                                                                                                          ggCGTAACCATGAAAGAGACGGCAAATCGTCCGTTGAAAGGTGCGACACCGGCGGCGTCAGATATACAGGAAGCGAAGGAAATTCTTGTCGAGCATTACGATAATATCGAGCAGAAAATCGATGATATTGACCACGAGATTGCCGATTTGCAGGCGAAACGTACCCGCCTGGTGCAGCAACATCCGCGAATTGATGAATAAGCTGAAACGGATGGCCTGATGTGACGCTGTCTTATCAGGCCAATTGAAct                                                                                  <  1:496545/251‑1 (MQ=255)                                                                                                                                                           gCGTAACCATGAAAGAGAGGGAAAATCGTCCGTTGAAAGGTGCGACACCGGGGGCGTCAGATATAAAGGAAGCGAAGGAAATTCTTGTCGAGCATTACGATAATATCGAGCAGAAAACCGATGATATTGACCACGAGATTGCCGATTTGCAGGCGAAACGTACCCGCCTGGTGCAGCAACATCCGCGAATTGATGAATAAGCTGAAACGGATGGCCTGATGTGACGCTGTCTTATCAGGCCAATTGAActc                                                                                 <  1:171279/251‑1 (MQ=255)                                                                                                                                                                    ggAAAGAGACGGCAAATCGTCCGTTGAAAGGTGCGACACCGGCGGCGTCAGATATACAGGAAGCGAAGGAAATTCTTGTCGAGCATTACGATAATATCGAGCAGAAAATCGATGATATTGACCACGAGATTGCCGATTTGCAGGCGAAACGTACCCGCCTGGTGCAGCAACATCCGCGAATTGATGAATAAGCTGAAACGGATGGCCTGATGTGACGCTGTCTTATCAGGCCAATTGAACTCTTAAGGTTc                                                                        <  1:10358/250‑1 (MQ=255)                                                                                                                                                                          agaCGGCAAATCGTCCGTTGAAAGGTGCGACACCGGCGGCGTCAGATATACAGGAAGCGAAGGAAATTCTTGTCGAGCATTACGATAATATCGAGCAGAAAATCGATGATATTGACCACGAGATTGCCGATTTGCAGGCGAAACGTACCCGCCTGGTGCAGCAACATCCGCGAATTGATGAATAAGCTGAAACGGATGGCCTGATGTGACGCTGTCTTATCAGGCCAATTGAACTCTTAAGGTTCACTTa                                                                   >  1:142929/1‑250 (MQ=255)                                                                                                                                                                           gaCGGCAAAACGTACGTTGAAAGGTGCGAAACCGGCGGCGGCAGATATACAGGAAGCGAAGGAAATTCTTGTCGAGAATTACGATAATATCGAGAAGAAAATCGATGATATTGACCACGAGATTGCCGATTTGCAGGCGAAACGTACCCGCCTGGTGCAGCAACATCCGCGAATTGATGAATAAGCTGAAACGGATGGCCTGATGTGACGCTGTCTTATCAGGCCAATTGAACTCTTAAGGTTCACTTAAt                                                                 <  2:142929/251‑1 (MQ=255)                                                                                                                                                                                 aaaTCGTCCGTTGAAAGGTGCGACACCGGCGGCGTCAGATATACAGGAAGCGAAGGAAATTCTTGTCGAGCATTACGATAATATCGAGCAGAAAATCGATGATATTGACCACGAGATTGCCGATTTGCAGGCGAAACGTACCCGCCTGGTGCAGCAACATCCGCGAATTGATGAATAAGCTGAAACGGATGGCCTGATGTGACGCTGTCTTATCAGGCCAATTGAACTCTTAAGGTTCACTTAAtctatg                                                            >  1:371606/1‑247 (MQ=255)                                                                                                                                                                                          gTTGAAAGGTGCGACACCGGCGGCGTCAGATATACAGGAAGCGAAGGAAATTCTTGTCGAGCATTACGATAATATCGAGCAGAAAATCGATGATATTGACCACGAGATTGCCGATTTGCAGGCGAAACGTACCCGCCTGGTGCAGCAACATCCGCGAATTGATGAATAAGCTGAAACGGATGGCCTGATGTGACGCTGTCTTATCAGGCCAATTGAACTCTTAAGGTTCACTTAATCTCTGACGCGCATAc                                                  >  1:134788/1‑251 (MQ=255)                                                                                                                                                                                                     cGACACCGGCGGCGTCAGATATACAGGAAGCGAAGGAAATTCTTGTCGAGCATTACGATAATATCGAGCAGAAAATCGATGATATTGACCACGAGATTGCCGATTGGCAGGCGAAATGTCCCCGCCTGGTGCAGCAACATCCGCGAATTGATGAATAAGCTGAAACGGATGGCCTGATGTGACGCTGTCTTATCAGGCCAATTGAACTCTTAAGGTTCACTTAATCTCTGACGCGCATACTCTCCTCCAgg                                       <  1:36855/251‑1 (MQ=255)                                                                                                                                                                                                            ggcggcGTCAGATATACAGGAAGCGAAGGAAATTCTTGTCGAGCCTTACGATAATATCGAGCAGAAAATCGATGATATTGACCACGCGATTGCCGATTTGCAGGCGAAACGTACCCGCCTGGTGCAGCAACATCCGCGAATTGGTGATTAAGCTGAAACGGATGGCCTGATGTGACGCTGTCTTATCAGGCCAATTGAACTCTTAAGGTTCACTTAATCTCTAACGCGCATACTCTCCTCAAGGTTAACgg                                >  1:1602/1‑251 (MQ=255)                                                                                                                                                                                                                    cAGATATACAGGAAGCGAAGGAAATTCTTGTCGAGCATTACGATAATATCGAGCAGAAAATCGATGATATTGACCACGAGATTGCCGATTTGCAGGCGAAACGTACCCGCCTGGTGCAGCAACATCCGCGAATTGATGAATAAGCTGAAACGGATGGCCTGATGTGACGCTGTCTTATCAGGCCAATTGAACTCTTAAGGTTAACTTAATCTCTGACGCGCATACTCTCCTCCAGGTTAACGGAGGAGAGt                        >  1:473792/1‑251 (MQ=255)                                                                                                                                                                                                                     aGATATACAGGAAGCGAAGGAAATTCTTGTCGAGCATTACGATAATATCGAGCAGAAAATCGATGATATTGACCACGAGATTGCCGATTTGCAGGCGAAACGTACCCGCCTGGTGCAGCAACATCCGCGAATTGATGAATAAGCTGAAACGGATGGCCTGATGTGACGCTGTCTTATCAGGCCAATTGAACTCTTAAGGTTCACTTAATCTCTGACGCGCATACTCTCCTCCAGGTTAACGGAGGAGAGTg                       >  1:72144/1‑251 (MQ=255)                                                                                                                                                                                                                       atatACAGGCAGAGAAGGAAATTCTTGTCGAACATTACGATAATATCGAGCAGAAAATCGATGATATTGACCACGAGATTGCCGATTTGCAGGCGAAACGTACCCGCCTGGTGCAGCAACATCCGCGAATTGATGAATAAGCTGAAACGGATGGCCTGATGTGACGCTGTCTTATCAGGCCAATTGAACTCTTAAGGTTCACTTAATCTCTGACGCGCATACTCTCCTCCAGGTTACCGGAGGAGAGTGc                      >  1:207402/1‑250 (MQ=255)                                                                                                                                                                                                                          taCAGGAAGCGAAGGAAATTCTTGTCGAGCATTACGATAATATCGAGCAGAAAATCGATGATATTGACCACGAGATTGCCGATTTGCAGGCGAAACGTACCCGCCTGGTGCAGCAACATCCGCGAATTGATGAATAAGCTGAAACGGATGGCCTGATGTGACGCTGTCTTATCAGGCCAATTGAACTCTTAAGGTTCACTTAATCTCTGACGCGCATACTCTCCTCCAGGTTAACGGAGGAGAGTGCAATg                  >  1:505691/1‑251 (MQ=255)                                                                                                                                                                                                                                          aaTTCTTGTCGAGAATTACGATAATATCGAGCAGAAAATCGATGATATTGCCCACGAGATTGCCGATTTGCAGGCGAAACGTACCCGCCTGGTGCAGCAACATCCGCGAATTGATGAATAAGCTGAAACGGATGGACTGATGTGACGCTGTCTTATCAGGCCAATTGAACTCTTAAGGTTCACTTAATCTCTGACGCGCATACTCTCCTCCAGTTTAACGGAGGATAGTGCAATGCAAAACCGTGTTTATg  >  2:491016/1‑251 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                     ATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAGAATCTGATGATGCCTGCCTATTACCTGATGGTAGTGGCGGTGGTTGGTTTAATCACCGGCGTAACCATGAAAGAGACGGCAAATCGTCCGTTGAAAGGTGCGACACCGGCGGCGTCAGATATACAGGAAGCGAAGGAAATTCTCGTCGAGCATTACGATAATATCGAGCAGAAAATCGATGATATTGACCACGAGATTGCCGATTTGCAGGCGAAACGTACCCGCCTGGTGCAGCAACATCCGCGAATTGATGAATAAGCTGAAACGGATGGCCTGATGTGACGCTGTCTTATCAGGCCAATTGAACTCTTAAGGTTCACTTAATCTCTGACGCGCATACTCTCCTCCAGGTTAACGGAGGAGAGTGCAATGAAAAACCGTGTTTATG  >  NC_000913/4331651‑4332134

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑