| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,320,007 | G→A | H219H (CAC→CAT) | phnI ← | carbon‑phosphorus lyase core complex subunit PhnI |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,320,007 | 0 | G | A | 94.5% | 194.2 / 0.5 | 76 | H219H (CAC→CAT) | phnI | carbon‑phosphorus lyase core complex subunit PhnI |
| Reads supporting (aligned to +/- strand): ref base G (2/2); new base A (40/29); total (45/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.40e-01 | |||||||||||
CGCCGTACTCCGGGGCTTGCAGAGCGCGGTCGACCAGCGCCATCGCCATCGCTTTGCGCTCGCTCATGCCGAACACCAGCCCGTAGCCGCGCGTGAAGTGCGGCGGCTCACCCGGCGGGTCGATAAAACCGTTAACCATTTCACACTCAGTCATCAGCAGTTCGCCGACGTTCACCGCAAATCCCAGCTCTTCCGGCACAATCGACACGTCGATATAGCCGCTGCGGATCTCGCCTGCGAACGGGTGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAG‑CGCCAGCAAATAGCCTTCGTCGCCGCGCATCAACTGCTGCAAACGGGAGGAGCGTGA‑GCAGGGGTAAACCGGCGGCGTGCGGGTGATGTCATC‑CGGCTGTGCGCCGCTATCCTCTTCAAACTTCGCCAGCCCCTGACGCGCCAGCAGGCTGAAAACGTGCGGCGACGGCTGCTGTTCGCTGTCGGCGGTGGTCAGCGTCGGCG > NC_000913/4319763‑4320248 | cGCCGTACTCCGGGGCTTGCAGAGCGCGGTCGACCAGCGCCATCGCCATCGCTTTGCGCTCGCTCATGCCGAACACCAGCCCGTAGCCGCGCGTGAAGTGCGGCGGCTCACCCGGCGGGTCGATAAAACCGTTAACCATTTCACACTCAGTCATCAGCAGTTCGCCGACGTTCACCGCAAATCCCAGCTCTTCCGGCACAATCGACACGTCGATATAGCCGCTGCGGATCTCGCCTGCGAACGGATGAtt < 1:79541/250‑1 (MQ=255) aCTCCGGGGCTTGCAGAGCGCGGTCGACCAGCGCCATCGCCATCGCTTTGCGCTCGCTCATGCCGAACACCAGCCCGTAGCCGCGCGTGAAGTGCGGCGGCTCACCCGGCGGGTCGATAAAACCGTTAACCATTTCACACTCAGTCATCAGCAGTTCGCCGACGTTCACCGCAAATCCCAGCTCTTCCGGCACAATCGACACTTCGATATAGCCGCTGCGGATCTCGCCTGCGAACGGATGATTGCGccc < 1:436324/250‑1 (MQ=255) aCTCCGGGGCTTGCAGAGCGCGGTCGACCAGCGCCATCGCCATCGCTTTGCGCTCGCTCATGCCGAACACCAGCCCGTAGCCGCGCGTGAAGTGCGGCGGCTCACCCGGCGGGTCGATAAAACCGTTAACCATTTCACACTCAGTCATCAGCAGTTCGCCGACGTTCACCGCAAATCCCAGCTCTTCCGGCACAATCGACACGTCGATATAGCCGCTGCGGATCTCGCCTGCGAACGGATGATTGCGCCCg < 2:79680/251‑1 (MQ=255) cTCCGGGGCTTGCAGAGCGCGGTCGACCAGCGCCATCGCCATCGCTTTGCGCTCGCTCATGCCGAACACCAGCCCGTAGCCGCGCGTGAAGTGCGGCGGCTCACCCGGCGGGTCGATAAAACCGTTAACCATTTCACACTCAGTCATCAGCAGTTCGCCGACGTTCCCCGCAAATCCCAGCTCTTCCGGCACAATCGACACGTCGATATAGCCGCTGCGGATCTCGCCTGCGAACGGATGATTGCGCCCGt > 1:310286/1‑251 (MQ=255) cTCCGGGGCTTGCAGAGCGCGGTCGACCAGCGCCATCGCCATCGCTTTGCGCTCGCTCATGCCGAACACCAGCCCGTAGCCGCGCGTGAAGTGCGGCGGCTCACCCGGCGGGTCGATAAAACCGTTAACCATTTCACACTCAGTCATCAGCAGTTCGCCGACGTTCACCGCAAATCCCAGCTCTTCCGGCACAATCGACACGTCGATATAGCCGCTGCGGATCTCGCCTGCGAACGGATGATTGCGCCCGt > 1:85012/1‑251 (MQ=255) ggggCTTGCAGAGCGCGGTCGACCAGCGCCATCGCCATCGCTTTGCGCTCGCTCATGCCGAACACCAGCCCGTAGCCGCGCGTGAAGTGCGGCGGCTCACCCGGCGGGTCGATAAAACCGTTAACCATTTCACACTCAGTCATCAGCAGTTCGCCGACGTTCACCGCAAATCCCAGCTCTTCCGGCACAATCGACACGTCGATATAGCCGCTGCGGATCTCGCCTGCGAACGGATGATTGCGCCCGTAAcc < 1:53343/251‑1 (MQ=255) ggCAGAGCGCGGTCGACCAGCGCCAGTGCCATCGCTTTGCGCTCGCTCATGCCGAACACCAGCCCGTAGCCGCGCGTGAAGTGCGGCGGCTCACCCGGCGGGTCGATAAAACCGTTACCCATTTCACACTCAGTCATCAGCAGTGCGCCGACGTTCACCGCAAATCCCAGCTCTTCCGGCACAATCGACACGTCGATATAGCCGCTGCGGATCTCGCCTGCGAACGGATGATTGCGCCCGTAACCGCGtt < 2:390406/249‑1 (MQ=255) gCAGAGCGCGGTCGACCAGCGCCATCGCCATCGCTTTGCGCTCGCTCATGCCGAACACCAGCCCGTAGCCGCGCGTGAAGTGCGGCGGCTCACCCGGCGGGGCGATAAAACCGTTAACCATTTCACACTCAGTCATCAGCAGATCGCCGAAGATTACAGCAAATCGCAGCTCTTCCGGCACAATCGAAACGTCGCTATAGCCGCTGCGGCTCTCGCCAGCGAACGGATGATTGCGCCCGTAACAGCGTTgg > 1:490095/1‑251 (MQ=255) gagCGCGGTCGACCAGCGCCATCGCCATCGCTTTGCGCTCGCTCATGCCGAACACCAGCCCGTAGCCGCGCGTGAAGTGCGGCGGCTCACCCGGCGGGTCGATAAAACCGTTAACCATTTCACACTCAGTCATCAGCAGTTCGCCGACGTTCACCGCAAATCCCAGCTCTTCCGGCACAATCGACACGTCGATATAGCCGCTGCGGATCTCGCCTGCGAACGGATGATTGCGCCCGTAACCGAGTTGGGTg > 1:49982/1‑251 (MQ=255) ggggggggccaccggcggcgccgccagcgcTTTTCGCTCGCTCATGCCGAACGCCGGCCCGGAGGCGCGCGTGAAGCGCGGCGGCTGACCCGGCGGGTCGATAAAGCCGTTAGCCATTTCACACGCAGTCATCGTCAGTTCGCCGACGTTCACCGCAAATCCCAGCTCTTCCGTCACAATCGACACGTCGATATAGCCGCTGCGGATCGCGCGGGCGAACGGATGATTGCTCCCGTAGCCTCGTTGGGTgg < 2:235611/230‑1 (MQ=255) cgcgGTCGACCAGCGCCATCGCCATCGCTTTGCGCGCGCTCATGCCGAACACCAGCCCGTAGCCGCGCGTGAAGTGCGGCGGCTCACCCGGCGGGTCGATCAAACCGTTAACCATTTCACACTCAGTCATCAGCAGTTCGCCGACGTTCACCGCAAATCCCAGCTCTTCCTGCACAATCGACACGTCGATATAGCCGCTGCGGATCTCGCCTGCGAACGGATGATTGCGCCCGTAACCGCGTTGGGTGGAg < 1:172732/251‑1 (MQ=255) cgGTCGACCAGCGTCATCGCCATCGCTTTGCGCTCGCTCATGCCGAACACCAGCCCGTAGCCGCGCGTGAAGTGCGGCGGCTCACCCGGCGGGTCGATAAAACCGTTAACCATTTCACACTCAGTCATCAGCAGTTCTCCGACGTTCACCGCAAATCCCAGCTCTTCCGGCACAATCGACACGTCGATATAGCCGCTGCGGATCTCGCCTGCGAACGGATGATTGCGCCCGTAACCGCGTTGGGTGGAGTa < 1:493477/251‑1 (MQ=255) ccgcccAGCGCCTGCGCCATCGCTTGGCGCTCGCGCATGCCGATCACCAGCCCGTAGCCGCGCGTGAAGTGCGGCGGCTCACCCGGCGGGCCGATAAAATCGTTAACCATTGCACGCTCAGTCATCAGCAGTTCGCCGACGTTCACCGCAAATCCCAGCGCTTCCGGCACAATCGCCACGTCGATATAGCCGCTGCGGATCTCGCCTGCGGACGGATGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGgc < 2:42150/247‑1 (MQ=255) ccAGCGCCATCGCCATCGCTTTGCGCTCGCTCATGCCGAACACCAGCCCGTAGCCGCGCGTGCAGTGCGGCGGCTCACCCGGCGGGTCGATAAAACAGTTAACCATTTCACACTCAGTCATCAGCAGTTCGCCGACGTTCAACGCAAATCCCAGCTCTTCCGGCACAATCGACACGTCGATATAGCCGCTGCGGATCTCGCCTGCGCACGGCTGATTGCGCCCGTCACCGCGTTGGGTGGCGTAGgccag‑c > 1:341737/1‑251 (MQ=255) cgccagcgcTTTGCGCTCGCTCATGCCGAACACCAGCCCGTAGCCGCGCGTGAAGTGCGGCGGCTCACCCGGCGTGTCGATAAAACCGTTAACCATTTCACACTCAGTCATCAGCAGTTCGCCGACGTTCACCGCAAATCCCAGCTCTTCCGGCACAATCGACACGTCGATATAGCCGCTGCGGATCTCGCCTGCGAACGGATGATTGCGCCCCTAACCGCGTTGGGTGGAGTAGGCCAG‑CGCCAGCaaa < 1:94002/250‑1 (MQ=255) gccatcgcTTTGCGCTCGCTCATGCCGAACACCAGCCCGTAGCCGCGCGTGAAGTGCGGCGGCTCACCCGGCGGGTCGATAAAACCGTTAACCATTTCACACTCAGTCATCAGCAGTTCGCCGACGTTCACCGCAAAACCCAGCCCTTCCGGCACAATCGACACGTCGATATAACCGCTGCGGATCTCGCCTGCGAACGGATTATTGCGCCCGTAACCGCGTTGGGTGGCGTAAGCCAG‑CGCCAGCAAAt > 1:400335/1‑250 (MQ=255) catcgcTTTGCGCTCGCTCATGCAGAACACCAGCCCGTAGCCGCGCGTGAAGTGCGGCGGCTCACCCGGCGGGTCGCTCAAACCATTAACCCTTTCCCAATCAGTCATCAGCAGTTCGCCGACGTTCCCAGCAAATCCCAGCCCTTCCGGCCCAATCGACCCGTCGATATAACCGCTGCGGGTCCCGCCTGCGCACGGCTGATTGCGCCCGTAACCGCGTTTGGGGGAGGAGgccag‑ggcctggaaaacac > 2:240869/1‑241 (MQ=255) gtttGCGCTCGCCCATGCCGAACACCAGCCCGTAGCCGCGCGTGAAGTGCGGCGGGTCACCCGGCGGGTCGACAAAACCGGTAACCATGTCACACTCAGTCAGCAGCAGTTCGCCGACGTTCACCGCAAATCCCAGCTCTTCCGGCACAATCGACACGTCGATATAGCCGCTGCGGATCTCGCCTGCGAACGGATGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAG‑CGCCAGCAAATAGCCTtcg < 2:108248/250‑1 (MQ=255) cgctcgctCATGCCGAACACCAGCCCGTAGCCGCGCGTGAAGTGCGGCGGCTCACCCGGCGGGTCGATAAAACCGTTAACCATTTCACACTCAGTCATCAGCAGTTCGCCGACGTTCACCGCAAATCCCAGCTCTTCCGGCACAATCGACACGTCGATATAGCCGCTGCGGATCTCGCCTGCGAACGGATGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAG‑CGCCAGCAAATAGCCTTCGTcgcc < 1:161425/251‑1 (MQ=255) gctcgctcATGCCGAACACCAGCCCGTAGCCGCGCGTGAAGTGCGGCGGCTCACCCGGCGGGGCGATAAAACCGTTAACCATTTCACACTCAGTCATCAGCAGTTCGCCGACGTTCACCGCAAATCCCAGCTCTTCCGGCACAATCGAAACGTCGATATAGCCGCTGCGGATCTCGCCTGCGAACGGATGATTGAGCCCGTAAACGCGTTGGGGGGAGTAGGCCAG‑CGCCAGCAAATAGCCTTCGTcgccc > 1:145668/1‑250 (MQ=255) gcgcgctcATGCCGAACACCAGCCCGTTGCCGCGCGTGAAGTGCGGCGGCTCACCCGGCGGGTCGATAAAACCGTTAACCATTGCACACTCAGTCAGCAGCAGTTCGCCGACGTTCACCGCAAAGCCCAGCTCTTCCGGCACAATCGACACGTCGAGATAGCCGCTGCGGATCTCGCCTGCGAACGGAGGATTGCGCCCGTAACCGCGTGGGGTGGAGTAGGCCAG‑CGCCAGCAAATAGCCTTCGTcgccg < 2:74221/248‑1 (MQ=255) cgctcATGCCGAACACCAGCCCGTAGCCGCGCGTGAAGTGCGGCGGCTCACCCGGCGGGTCGATAAAACCGTTAACCATTTCACACTCAGTCATCAGCAGTTCGCCGACGTTCACCGCAAATCCCAGCTCTTCCGGCACAATCGACACGTCGATATAGCCGCTGCGGATCTCGCCCGCGAACGGATGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGACAG‑CGCCAGCAAATAGCCTTCGTCGCCgcgc > 1:213255/1‑251 (MQ=255) gctcATGCCGAACACCAGCCCGTAGCCGCGCGTGAAGTGCGGCGGCTCACCCGGCGGGTCGATAAAACCGTTAACCATTTCACACTCAGTCATCAGCAGTTCGCCGACGTTCACCGCAAATCCCAGCTCTTCCGGCACAATCGACACGTCGATATAGCCGCTGCGGATCTCGCCTGCGAACGGATGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAG‑CGCCAGCAAATAGCCTTCGTCGCCGCGCa < 1:309679/251‑1 (MQ=255) gAACACCAGCCCGTAGCCGCGCGTGACGTGCGGCGGCTCGCCCGGCGGGTCGTTAAAACCGTTAACCATTTCACCCTCAGTCATCAGCAGTTCGCCGACGTTCACCGCAAATCCCAGCTCTTCCGGCACAATCGACACGTCGATATAGCCGCTGCGGATCTCGCCTGCGAACGGGTGATTGCGCCCGTAAcc > 2:150916/1‑192 (MQ=255) gAACACCAGCCCGTAGCCGCGCGTGAAGTGCGGCGGCTCGCCCGGCGGGTCGTTAAAACCGTTAACCATTTCACACTCAGTCATCAGCAGTTCGCCGACGTTCACCGCAAATCCCAGCTCTTCCGGCACAATCGACACGTCGATATAGCCGCTGCGGATCTCGCCTGCGAACGGGTGATTGCGCCCGTAAcc < 1:150916/192‑1 (MQ=255) aGCCGCGCGTGAAGTGCGGCGGCTCACCCGGCGGGTCGATAAAACCGTTAACCATTTCACACTCAGTCATCAGCAGTTCGCCGACGTTCACCGCAAATCCCAGCTCTTCCGGCACAATCGACACGTCGATATAGCCGCTGCGGATCTCGCCTGCGAACGGATGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAG‑CGCCAGCAAATAGCCTTCGTCGCCGCGCATCAACTGCTGCAAACGGgaggag < 1:263178/251‑1 (MQ=255) cggcTCACCCGGCGGGTCGATAAAACCGTTAACCATTTCACACTCAGTCATCAGCAGTTCGCCGACGTTCACCGCAAATCCCAGCTCTTCCGGCACAATCGACCCGTCGATATAGCCGCTGCGGATCTCGCCTGCGAACGGATGATTGCGCCCGTAACCGCGTTGGGGGGAGTAGGCCAGACACC‑GCAAATAGCCTTCGTCGCCGCGCCTCCACTGCTGCAAAAGGGAGGAGGGTGA‑GCAGGGGGaaaacg > 2:378936/1‑248 (MQ=255) cggcTCACCCGGCGGGTCGATAAAACCGTTAACCATTTCACACTCAGTCATCAGCAGTTCGCCGACGTTCACCGCAAATCCCAGCTCTTCCGGCACAATCGACACGTCGATATAGCCGCTGCGGATCTCGCCTGCGAACGGATGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAG‑CGCCAGCAAATAGCCTTCGTCGCCGCGCATCAACTGCTGCAAACGGGAGGAGCGTGA‑GCAGGGGTAAACcg < 2:214208/251‑1 (MQ=255) ggcTCACCCGGCGGGTCGATAAAACCGTTAACCATTTCACACTCAGTCATCAGCAGTTCGCCGACGTTCACCGCAAATCCCAGCTCTTCCGGCACAATCGACACGTCGATATAGCCGCTGCGGATCTCGCCTGCGAACGGATGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAG‑CGCCAGCAAATAGCCTTCGTCGCCGCGCATCAACTGCTGCAAACGGGAGGAGCGTGA‑GCAGGGGTAAACcgg > 1:250112/1‑251 (MQ=255) cACCCGGCGGGTCGATAAAACCGTTAACCATTTCACACTCAGTCATCAGCAGTTCGCCGACGTTCACCGCAAATCCCAGCTCTTCCGGCACAATCGACACGTCGATATAGCCGCTGCGGATCTCGCCTGCGAACGGATGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAG‑CGCCAGCAAATAGCCTTCGTCGCCGCGCATCAACTGCTGCAAACGGGAGGAGCGTGA‑GCAGGGGTAAACCggcggc < 1:192964/251‑1 (MQ=255) aCCCGGCGGGTCGATAAAACCGTTAACCATTTCACACTCAGTCATCAGCAGTTCGCCGACGTTCACCGCAAATCCCAGCTCTTCCGGCACAATCGACACGTCGATATAGCCGCTGCGGATCTCGCCTGCGAACGGATGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAG‑CGCCAGCAAATAGCCTTCGTCGCCGCGCATCAACTGCTGCAAACGGGAGGAGCGTGA‑GCAGGGGAAAACCGgcggcg > 2:166006/1‑251 (MQ=255) cccGGCGGGTCGATAAAACCGTTAACCATTTCACACTCAGTCATCAGCAGTTCGCCGACGTTCACCGCAAATCCCAGCTCTTCCGGCACAATCGACACGTCGATATAGCCGCTGCGGATCTCGCCTGCGAACGGATGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAG‑CGCCAGCAAATAGCCTTCGTCGCCGCGCATCAACTGCTGCAAACGGGAGGAGCGTGA‑GCAGGGGTAAACCGGCGGCGt < 1:295874/251‑1 (MQ=255) ccGGCGGGTCGATAAAACCGTTAACCATTTCACACTCAGTCATCAGCAGTTCGCCGACGTTCACCGCAAATCCCAGCTCTTCCGGCACAATCGACACGTCGCTATAGCCGCTGCGGATCTCGCCTGCGAACGGATGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAG‑CGCCAGCAAATAGCCTTCGTCGCCGCGCATCAACTGCTGCAAACGGGAGGAGCGTGA‑GCAGGGGTAAACCGGCGGCGTg > 1:471664/1‑251 (MQ=255) ggcggGACGATAAAACCGTTAAACCTTTCAACCTAAGGCAAAAGCAGTTAGCCGACGTTCAACGCAAATCCCAGCTCTTACGGCACAATCGACACGTCGATATAGCCGCTGCGGATCTCGACTGCGAACGGAGGATTACGCCCGTAACCGCGTTGGGGAGAGTAGGCCAG‑CGCCAGCAAAAAGCCTGAGTCGCCGCGCATCAACTGCTGCGAACGGGCGGAG‑ATGAAGCAGGGGTAAAACGggggcgaggg > 2:337010/1‑247 (MQ=255) tCGATAAAACCGTTAACCATTTCACACTCAGTCATCAGCAGTTCGCCGACGTTCACCGCAAATCCCAGCTCTTCCGGCACAATCGACACGTCGATATAGCCGCTGCGGATCTCGCCTGCGAACGGATGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAG‑CGCCAGCAAATAGCCTTCGTCGCCGCGCATCAACTGCTGCAAACGGGAGGAGCGTGA‑GCAGGGGGAAACCGGCGGCGTGCGGGTGAt > 1:174100/1‑251 (MQ=255) aaCCGTTAACCATTTCACACTCAGTCATCAGCAGTTCGCCGACGTTAACCGAAAATCCCAGCTCTTCCGGCACAATCGACACGTCGATATAGCCGCTGCGGCTCTCGCCCGCGAACGGATGATCGCGCCCGTAACCCCGTTGGGTGGGGTAGGGCAG‑CGCCAACAAATAGCCTACGACGCCGCACCTCCACTGCTGCCAACGGGAGGAGCGTGA‑GCAGGGTTCAACAGACGGCGTGCAGGTAATgacgtcc > 1:490022/1‑245 (MQ=255) aaCCGTTAACCATTTCACACTCAGTCATCAGCAGTTAGCCGACGTTCACCGCAAATCCCAGCTCTTCCGGCACAATCGACCAGTCGATATAGCCGCTGCGGATCTCGCCTGCGAACGGATGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAG‑AGCCAGCAAAGAGCCTTCATCGACGCGCATCAACTGCTGCAAAAGGGAGGAGCGTGA‑GCAGGGGTAAACCGGCGGCGGGCGGGGGATGTCATc‑c > 1:490002/1‑251 (MQ=255) aCCGTTAACCATTTCACACTCAGTCATCAGCAGTTCGCCGACGTTCACCGCAAATCCCAGCTCTTCCGGCACAATCGACACGTCGATATAGCCGCTGCGGATCTCGCCTGCGAACGGATGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAG‑CGCCAGCAAATAGCCTTCGTCGCCGCGCATCAACTGCTGCAAACGGGAGGAGCGTGA‑GCAGGGGTAAACCGGCGGCGTGCGGGTGATGTCATC‑Cg > 1:45755/1‑251 (MQ=255) ccGTTAACCATTCCACACGCAACCACCAGCTGTTCTCCTACTTTCACCGCAAATCCCAGCTCTGCCGGCACAATCGACACGTCGATATAGCCTCTGCGGATCTCGCCTGCGAACGGATGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAG‑CGCCAGCAAATAGCCTTCGTCGCCGCGCATCAACTGCTGCAAACGGGAGGAGCGTGA‑GCAGGGGTAATCCGGCGGCGTGCTGGTGATGTCAGC‑Cgg < 2:400335/251‑1 (MQ=255) tAACCCTTTCACACTCAGTCATCAGCAGTTCGCCGACGTTCACCGCAAATCCCAGCTCTTCCGGCCCAATCGACACGTCGATATAGCCGCTGCGGATCTCGCCTGCGCACGGATGATTGCGCCCGTAACCGCGTTGGGAGGAGCAGGCCAG‑CGACAGCAAATAGCCTTCGTCGCCGCGCATCAACTGCTGCAAACAGGAGGAGCGTGA‑GCAGGGGTAATCCGGCGGCGGGCGGGGGACGTCACC‑CGGCtgt > 2:238365/1‑251 (MQ=255) acaaTTTAACACTCAGTCATCAGCAGTTCGCCGTCCTTCACCGAAAATCCCAGCTCTTCCGGCAAAATAGACACGTCGATATAGCCGCAGCGGATCTCGCCTGCGAACGGGTGATTGCGCCCGTACCCGAGTTGGGTGGAGTAGGCCAG‑AGCCAGCAAATAGCCTTCGACGCCGCGCATCAGCTGCTGTAGACGGGGGGAGGGGGA‑GCCGGGGTACACCGGCAGCGTGCgggagcgagcaaccggccgggc > 2:27589/4‑232 (MQ=255) aCCATTTCACACTCAGTCATCAGCAGTTCGCCGACGTTCACCGCAAATCCCAGCTCTTCCGGCACAATCGACACGTCGATATAGCCGCTGCGGATCTCGCCTGCGAACGGATGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAG‑CGCCAGCAAATAGCCTTCGTCGCCGCGCATCAACTGCTGCAAACGGGAGGAGCGTGA‑GCAGGGGTAAACCGGCGGCGTGCGGGTGATGTCATC‑CGGCTGTgc < 1:421839/251‑1 (MQ=255) cacaCTCAGTCATCAGCAGTTCGCCGACGTTCACCGCAAATCCCAGCTCTTCCGGCACAATCGACACGTCGATATAGCCGCTGCGGATCTCGCCTGCGAACGGATGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAG‑CGCCAGCAAATAGCCTTCGTCGCCGCGCATCAACTGCTGCAAACGGGAGGAGCGTGA‑GCAGGGGTAAACCGGCGGCGTGCGGGTGATGTCATC‑CGGCTGTGCGCCGCt > 1:445778/1‑250 (MQ=255) cAGTCATCAGCAGTTCGCCGTCGTTCACCGCAAATCCCAGCTCTTCCGGCACACTCGACACGTCGATATAGCCGCTGCGGATCTCGCCTGCGAACGGATGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAG‑CGCCAGCAAATAGCCTTCGTCGCCGCGCATCCACTTCTGCAAACGGGAGGAGCGGGA‑GCAGGGGTAAACCGGCGGCGTGCGGGTGATGTCATC‑CGGCGGTGcgccgataaccact > 2:366598/1‑242 (MQ=255) gTCATCAGCAGTTCGCCGACGTTCACCGCAAATCCCAGCTCTTCCGGCACAATCGACACGTCGATATAGCCGCTGCGGATCTCGCCTGCGAACGGATGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAG‑CGCCAGCAAATAGCCTTCGTCGCCGCGCATCAACTGCTGCAAACGGGAGGAGCGTGA‑GCAGGGGCAAACCGGCGGCGTGAGGGTGATGTCATC‑CGGCTGTGCGCCGCTATCCTCTTc > 2:132534/1‑251 (MQ=255) tcatcaGCAGTTCGCCGACGTTCACCGCAAATCCCAGCTCTTCCGGCACAATCGACACGTCGATATAGCCGCTGCGGATCTCGCCTGCGAACGGATGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAG‑CGCCAGCAAATAGCCTTCGTCGCCGCGCATCAACTGCTGCAAACGGGAGGAGCGTGA‑GCAGGGGTAAACCGGCGGCGTGCGGGTGATGTCATC‑CGGCTGGGCGCCGCTATCCTCTTc > 2:285431/1‑250 (MQ=255) catcaGCAGTTCGCCGACGTTCACCGCAAATCCCAGCTCTTCCGGCACAATCGACACGTCGATATAGCCGCTGCGGATCTCGCCTGCGAACGGATGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAG‑CGCCAGCAAATAGCCTTCGTCGCCGCGCATCAACTGCTGCAAACGGGAGGAGCGTGA‑GCAGGGGTAAACCGGCGGCGTGCGGGTGATGTCATC‑CGGCTGTGCGCCGCTATCCTCTTCa > 1:487466/1‑250 (MQ=255) atcaGCAGTTCGCCGACGTTCACCGCAAATCCCAGCTCTTCCGGCACAATCGACCCGTCGATATAGCCGCTGCGGATCTCGCCTGCGAACGGATGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAG‑CGCCAGCAAATAGCCTTCGTCGCCGCGCATCACCTGCTGCAACCGGGCGGAGCGTGC‑GCAGGGGTAAACCGGCGGCGGGCGGGGGCGGCCATC‑CGGCTGGGCGCCGCTCTCCTCTTCaa > 2:279162/1‑250 (MQ=255) cagTTCGCCGACGTTCACCGCAAATCCCAGCTCTTCCGGCACAATCGACACGTCGATATAGCCGCTGCGGATCTCGCCTGCGAACGGATGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAG‑CGCCAGCAAATAGCCTTCGTCGCCGCGCATCAACTGCTGCAAACGGGAGGAGCGTGA‑GCAGGGGTAAACCGGCGGCGTGCGGGTGATGTCATC‑CGGCTGTGCGCCGCTATCCTCTTCAAACTTCg > 1:162606/1‑251 (MQ=255) gtgcGCCGACGGTCACCGCAAATCCCAGCTCTTCCGGCACAATCGACTCGGCGATATAGCCGCTGCGGATCTCGCCTGCGAACGGATGATTGCGCCCGTAACCGCGTTGGGTTGAGTAGGCCAG‑CGCCAGCAAATAGCCTTCGTCGCCGCGCATCAACTGCTGCAAACGGGAGGAGCGTGA‑GCAGGTGTAAACCGGCGGTGTGCGGGTGATGTCATC‑CGGCTGTGCGCCGCTATCCTCTTCAAACTTCGcc < 2:213255/248‑1 (MQ=255) gTTCGCCGACGTTCACCGCAAATCCCAGCTCTTCCGGCACAATCGACACGTCGATATAGCCGCTGCGGATCTCGCCTGCGAACGGATGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAG‑CGCCAGCAAATAGCCTTCGTCGCCGCGCATCAACTGCTGCAAACGGGAGGAGCGTGA‑GCAGGGGTAAACCGGCGGCGTGCGGGTGATGTCATC‑CGGCTGTGCGCCGCTATCCTCTTCAAACTTCGcc < 1:12992/251‑1 (MQ=255) ttCGCCGACGTTCACCGCAAATACCAGATCTTCCGGCACAATCGACACGTCGCTATAGCCGCTGCGGATCTCGCCTGCGAACGGATGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAG‑CGCCACCAAATAGCCTTCGTCGCCGCGCCTCACCAGCTGGAAACGGGAGGACAGTGA‑GCAGGGATAAGACGGCGGCAGGCGGGTGATGTC‑TCACGTATGTGCGCCGCTATcccctcccaactgctca > 2:367086/1‑235 (MQ=255) cGCCGACGTTCACCGCAAATCCCAGCTCTTCCGGCACAATCGACACGTCGATATAGCCGCTGCGGATCTCGCCTGCGAACGGATGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAG‑CGCCAGCAAATAGCCTTCGTCGCCGCGCATCAACTGCTGCAAACGGGAGGAGCGTGA‑GCAGGGGTAAACCGGCGGCGTGCGGGTGATGTCATC‑CGGCTGTGCGCCGCTATCCTCTTCAAACTTCGCCAGc > 1:421564/1‑251 (MQ=255) gCCGACGTTCACCGCAAATCCCAGCTCTTCCGGCACAATCGACACGTCGATATAGCCGCTGCGGATCTCGCCTGCGAACGGGTGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAG‑CGCCAGCAAATAGCCTTCGTCGCCGCGCATCAACTGCTGCAAACGGGAGGAGCGTGA‑GCAGGGGTAAACCGGCGGCGTGCGGGTGATGTCATC‑CGGCTGTGCGCCGCTATCCTCTTCAAACTTCGCCAGcc < 2:157567/251‑1 (MQ=255) cGACGTTCACCGCAAATCCCAGCTCTTCCGGCACAATCGACACGTCGATATAGCCGCTGCGGATCTCGCCTGCGAACGGATGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAG‑CGCCAGCAAATAGCCTTCGTCGCCGCGCATCAACTGCTGCAAACGGGAGGAGCGTGA‑GCAGGGGTAAACCGGCGGCGTGCGGGTGATGTCATC‑CGGCTGTGCGCCGCTATCCTCTTCAAACTTCGCCAGcccc > 1:346676/1‑251 (MQ=255) aCGTTCACCGCAAATCCCAGCTCTTCCGGCACAATCGACACGTCGATATAGCCGCTGCGGATCTCGCCTGCGAACGGATGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAG‑CGCCAGCAAATAGCCTTCGTCGCCGCGCATCAACTGCTGCAAACGGGAGGAGCGTGA‑GCAGGGGTAAACCGGCGGCGTGCGGGTGATGTCATC‑CGGCTGTGCGCCGCTATCCTCTTCAAACTTCGCCAGCCCCTg > 1:446268/1‑251 (MQ=255) gTTCACCGCAAATCCCAGCTCTTCCGGCACAATCGACACGTCGATATAGCCGCTGCGGATCTCGCCTGCGCACGGATGATTGGGCCCGTAACCGCGGTGGGTGGAGTAGGCCAG‑CGCCATCAAATAGCCTTCGTCGCCGCGCATCAACTGCAGCAAACGGGAGGAGCGTGA‑GCCGGGGTAAACCGGCGGCGTGCGGGTGATGTCAGC‑CGGCTGTGCGCCGCTATCCTCTTCAGACTTCGCCAGCCACTg > 1:346010/1‑249 (MQ=255) cACCGCAAATCCCCGCTCTCCCGGCACAACCGACTCGTCGATATAGCCGCTGCGGATCTCGCCTGCGAACGGATGATTGCGCCCGGAACCGCGTTGGGTGGAGTAGGCCAG‑CGCCAGCAAATAGCCTTCGTCGCCGCGCATCAACTGCTGCAAACGGGAGGAGCGTGA‑GCAGGGGTAAACCGGCGGCGTGCGGGTGATGTCATC‑CGGCTGTGCGCCGCTATCCTCTTCAAACTTCGCCAGCCCCTGAcgcg < 2:421564/251‑1 (MQ=255) cACCGCAAATCCCAGCTCTTCCGGCACAATCGACACGTCGATATAGCCGCTGCGGATCTCGCCTGCGAACGGATGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAG‑CGCCAGCAAATAGCCTTCGTCGCCGCGCATCAACTGCTGCAAACGGGAGGAGCGTGA‑GCAGGGGTAAACCGGCGGCGTGCGGGTGATGTCATC‑CGGCTGTGCGCCGCTATCCTCTTCAAACTTCGCCAGCCCCTGAcgcg > 1:57881/1‑251 (MQ=255) gCAAATCCCAGCTCTTCCGGCACAATCGACACGTCGATATAGCCGCTGCGGATCTCGCCTGCGAACGGATGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAG‑CGCCAGCAAATAGCCTTCGTCGCCGCGCATCAACTGCTGCAAACGGGAGGAGCGTGA‑GCAGGGGTAAACCGGCGGCGTGCGGGTGATGTCATC‑CGGCTGTGCGCCGCTATCCTCTTCAAACTTCGCCAGCCCCTGACGCGCcag < 1:337010/251‑1 (MQ=255) gCAAATCCCAGCTATTCCGGCACAATCGACACGTCGATATAGCCGCTGCGGATCTCTCCTGCGAACGGATAATTGAGCCCGTAAACGCGTGAGGTGGATTAGGCCAG‑CACCAGCAAATAGCCTTCATCGCCCCGCATCACATGCGGCAAACGGGAGGACCGTGC‑GCAGGGGTAACCCGGAGGCGAGCGTGTTATGGCATC‑CGGCTGTGCGCCGATGTCCTATGGAAACTTCGCCAGCCCCTGAcgagca > 2:339665/1‑245 (MQ=255) acaTCCCAGCTCTTCCGGCACAACCGACACGTCGATATAGCCGCTGCGGATCTCGCCTGCGAACGGATGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAG‑CGCCAGCAAATAGCCTTCGTCGCCGCGCATCAGCTGCTGCAAACGGGAGGAGCGTGA‑GCAGGGGTAAACCGGCGGCGTGCGGGTGATGTCATC‑CGGCTGTGCGCCGCTATCCTCTTCAAACTTCGCCAGCCCCTGACGCGCcagca < 1:421850/249‑1 (MQ=255) ctctTCCGGCACAATCGACACGTCGATATAGCCGCTGCGGATCTCGCCTGCGAACGGATGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAG‑CGCCAGCAAATAGCCTTCGTCGCCGCGCATCAACTGCTGCAAACGGGAGGAGCGTGA‑GCAGGGGTAAACCGGCGGCGTGCGGGTGATGTCATC‑CGGCTGTGCGCCGCTATCCTCTTCAACCTTCGCCAGCCCCTGCCGCGCCAGCAGGCTgcaa > 2:114883/1‑247 (MQ=255) aaTCGACACGTCGATATAGCCGCTGCGGATCTCGCCTGCGAACGGATGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAG‑CGCCAGCAAATAGCCTTCGTCGCCGCGCATCAACTGCTGCAAACGGGAGGAGCGTGA‑GCAGGGGTAAACCGGCGGCGTGCGGGTGATGTCATC‑CGGCTGTGCGCCGCTATCCTCTTCCAACTTCGCCAGCCCCTGACGCGCCAGCAGGCTGCAAACGTgcggcgccg > 2:178229/1‑248 (MQ=255) cGACACGTCGATATAGCCGCTGCGGATCTCGCCTGCGAACGGATGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAG‑CGCCAGCAAATAGCCTTCGTCGCCGCGCATCAACTGCTGCAAACGGGAGGAGCGTGA‑GCAGGGGTAAACCGGCGGCGTGCGGGTGATGTCATC‑CGGCTGTGCGCCGCTATCCTCTTCAAACTTCGCCAGCCCCTGACGCGCCAGCAGGCTGAAAACGTGCGGCGACGgc < 1:442540/250‑1 (MQ=255) cGACACGGCGATATAGCCGCTGCGGATATCGCCTGCGAACGGATAATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCCG‑CGCCAGCAAATAGCCTTCGTCGCCGCGCATCAACTGCTGCAACCGGGAGGAGCGTGA‑GCGGGGGTAAACAGGCGGCGTGCGGGTGATGTCATC‑CGGCTGTGCGCCGCTATCCTCTTCACCATTCGCCAGCCCCTGACGCGCCAGCCGGCTGAAAACGTGCGGCGACGgcg > 2:39367/1‑250 (MQ=255) gACACGTCGATATAGCCGCTGCGGATCCCGCCTGCGACCGGATGATGGCGCCCGTACCCGCGTTGGGTGGAGTAGGCCAG‑CGCCAGCAAATAGCCTTCGTCGCCGCGCATCAACTGCTGCAAACGGGAGGAGCGTGA‑GCAGGGGTAAACCGGCGGCGTGCGGGTGATGTCATC‑CGGCTGTGCGCCGCGATCCTCTTCAAACTTCGCCAGCCCCTGACGCGCCAGCAGGCTGAAAACGTGCGGCGACGgctg < 2:145668/251‑1 (MQ=255) gACACGTCGATATAGCCGCGGCGGATCTCGCCTGCGAACGGATGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAG‑CGCCAGCAAATAGCCTTCGTCGCCGCGCATCAACTGCTGCAAACGGGAGGAGCGTGA‑GCAGGGGTAAACCGGCGGCGTGCGGGTGATGTCATC‑CGGCTGTGCGCCGCTATCCTCTTCAAACTTCGCCAGCCCCTGACGCGCCAGCAGGCTGAAAACGTGCGGCGACGgctg < 1:430316/251‑1 (MQ=255) tataGCCGCTGCGGATCTCGCCTGCGAACGGATGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAG‑CGCCAGCAAATAGCCTTCGTCGCGGCGCATCAACTGCTGCAAACGGGAGGAGCGTGA‑GCAGGGGTAAACCGGCGGCGTGCGGGTGATGTCATC‑CGGCTAGGCGCCGCTATCCTCTTCAAACTTCGCCAGCCACTGACGCGCCAGCAGGCCGAAAACGGGCGGCGGCGGCTGCTGTTCGCTg > 2:125948/1‑251 (MQ=255) aGCCGCTGCGGATCTCGCCTGCGAACGGATGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAG‑CGCCAGCAAATAGCCTTCGTCGCCGCGCATCAACTGCTGCAAACGGGAGGAGCGTGA‑GCAGGGGTAAACCGGCGGCGTGCGGGTGATGTCATC‑CGGCTGTGCGCCGCTATCCTCTTCAAACTTCGCCAGCCCCTGACGCGCCAGCAGGCTGAAAACGGGCGGCGACGGCTGCTGTTCGCTGTcg > 1:152096/1‑251 (MQ=255) gCCGCTGCGGATCTCGCCTGCGAACGGATGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAG‑CGCCAGCAAATAGCCTTCGTCGCCGCGCATCAACTGCTGCAAACGGGAGGAGCGTGA‑GCAGGGGTAAACCGGCGGCGTGCGGGTGATGTCATC‑CGGCTGTGCGCCGCTATCCTCTTCAAACTTCGCCAGCCCCTGACGCGCCAGCAGGCTGACAACGTGCGGCGCCGGCTGCTGTTCGCTGGcgg > 2:251468/1‑251 (MQ=255) cGCCGGCGAACGGATGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAG‑CGCCAGCAAATAGCCTTCGTCGCCGCGCATCAACTGCTGCAAACGGGAGGAGCGTGA‑GCAGGGGTAAACCGGCGGCGTGCGGGTGATGTCATC‑CGGCTGTGCGCCGCTATCCTCTTCAAACTTCGCCAGCCCCTGACGCGCCAGCAGGCTGAAAACGTGCGGCGACGGCTGCTGTTCGCTGTCGGCGGTGGTCAGCGTc < 2:169982/251‑1 (MQ=255) ccTGCGAACGGATGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAG‑CGCCAGCAAATAGCCTTCGTCGCCGCGCATCAACTGCTGCAAACGGGAGGAGCGTGA‑GCAGGGGTAAACCGGCGGCGTGCGGGTGATGTCATC‑CGGCTGTGCGCCGCTATCCTCTTCAAACTTCGCCAGCCCCTGACGCGCCAGCAGGCTGAAAACGTGCGGCGACGGCTGCTGTTCGCTGTCGGCGGTGGTCAGCGTCgg > 1:435694/1‑251 (MQ=255) ccTGCGAACGGATGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAG‑CGCCAGCAAATAGCCTTCGTCGCCGCGCATCAACTGCTGCAAACGGGAGGAGCGTGA‑GCAGGGGTAAACCGGCGGCGTGCGGGTGATGTCATC‑AGGCTGTGCGCCGCTATCCTCTTCAAACTTCGCCAGCCCCTGACGCGCCAGCAGGCTGAAAACGAGCGGCGACGGCTGCTGTTCGCTGTCGGCGGGAGTCAGCGTCgg > 1:285380/1‑251 (MQ=255) cTGCGAACGGATGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAG‑CGCCAGCAAATAGCCTTCGTCGCCGCGCATCAACTGCTGCAAACGGGAGGAGCGTGA‑GCAGGGGTAAACCGGCGGCGTGCGGGTGATGTCATC‑CGGCTGTGCGCCGCTATCCTCTTCAAACTTCGCCAGCCCCTGACGCGCCAGCAGGCTGAAAACGTGCGGCGACGGCTGCTGCTCGCTGTCGGCGGTGGTCAGCGTCggg > 2:113479/1‑250 (MQ=255) tGCGAACGGTTGATTGCGACCGTAACCGCGTTGTGTGGAGTAGGCAAG‑CGCCAGCAAATAGCCTTCGTCGCCGCGCATCAAATACTGCACACGGGAGGAGCGTGA‑GCAGGGGTAGACCGGCGTCTTGCGGGTGATGTCATC‑CGGCTGTGCGCCGCTATCCTCTTCAAACTTCGCCAGCCCCTGACGCGCCCGCAGGCTGAAAACGCGCAGCGACGCCAGCGGTTTGCTGTctgcgtagcgtaactgcggcg > 2:24737/1‑235 (MQ=255) | CGCCGTACTCCGGGGCTTGCAGAGCGCGGTCGACCAGCGCCATCGCCATCGCTTTGCGCTCGCTCATGCCGAACACCAGCCCGTAGCCGCGCGTGAAGTGCGGCGGCTCACCCGGCGGGTCGATAAAACCGTTAACCATTTCACACTCAGTCATCAGCAGTTCGCCGACGTTCACCGCAAATCCCAGCTCTTCCGGCACAATCGACACGTCGATATAGCCGCTGCGGATCTCGCCTGCGAACGGGTGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAG‑CGCCAGCAAATAGCCTTCGTCGCCGCGCATCAACTGCTGCAAACGGGAGGAGCGTGA‑GCAGGGGTAAACCGGCGGCGTGCGGGTGATGTCATC‑CGGCTGTGCGCCGCTATCCTCTTCAAACTTCGCCAGCCCCTGACGCGCCAGCAGGCTGAAAACGTGCGGCGACGGCTGCTGTTCGCTGTCGGCGGTGGTCAGCGTCGGCG > NC_000913/4319763‑4320248 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |