Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,307,151	G→A	P217S (CCT→TCT)	alsK ←	D‑allose kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,307,151	0	G	A	96.4%	157.0 / ‑6.1	55	P217S (CCT→TCT)	alsK	D‑allose kinase
Reads supporting (aligned to +/- strand):  ref base G (0/2);  new base A (27/26);  total (27/28)
Fisher's exact test for biased strand distribution p-value = 4.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGAC‑GAAAAGATCGCG‑CAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCA‑GTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCG  >  NC_000913/4306907‑4307383                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                            tGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCAGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGTAGACTCTGGACGAAAGAGGCGtt                                                                                                                                                                                                                                       >  1:394053/1‑251 (MQ=255)   aTGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCAGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGAGGCGtttt                                                                                                                                                                                                                                     <  1:134647/251‑1 (MQ=255)     gcgcCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCAGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGAGGCGTTTTcc                                                                                                                                                                                                                                   <  2:190549/251‑1 (MQ=255)          aaTATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCAGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGAGGCGTTTTCCGCATg                                                                                                                                                                                                                              >  1:243282/1‑251 (MQ=255)             atTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCAGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGAGGCGTTTTCCGCATGGAc                                                                                                                                                                                                                           >  1:38054/1‑251 (MQ=255)               tgctgcACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCAGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGCAGGCGGGCATATCCATCAAGCCACCGCCCAGGATCAACGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGAGGCGTTTTCCGCATggc                                                                                                                                                                                                                            >  1:463238/1‑247 (MQ=255)                        cTGAGCGCCATTAAAGACAGAAGATGCGGCAGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCCCGCCACCGCCCAGGATCACCGCATCGGGGAAGAACAGATTAATGCTGGTGGCAATGGCCCGCGCCGCGTTTTCAAGCAGACTCTGGACGAAAGAGGCGTTTTCCCCATGTAC‑GGAAAGAAcgc                                                                                                                                                                                                               >  2:383078/1‑251 (MQ=255)                             cgcCATTAAAGACAGAAGATGAGGCAGCAATACAGCGCACGACCTGATGCGGAAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGAGGCGATTTCCGCATGGAC‑GAAAAGATCGCG‑CAAt                                                                                                                                                                                                         >  1:190774/1‑251 (MQ=255)                                 aTTAAAGTAAGAAGATGAGGCAGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGAGGCGTTTTCCGCATGGAC‑GAAAAGATCGCG‑CAATGGGt                                                                                                                                                                                                     <  2:264307/251‑1 (MQ=255)                                   tAAAGTCAGAAGATGAGGCAGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGAGGCGTTTTCCGCATGGAC‑GAAAAGATCGCG‑CAATGGGTa                                                                                                                                                                                                    <  1:253185/250‑1 (MQ=255)                                      aGTCAGAAGATGAGGCAGCAGTAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGATAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCTCCGCAGCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAATCAGTCTCTGGACGAAAGAGGCGTTTTCCGCATGGAC‑GAAAAGATCGCG‑CAATGGGTAAtgt                                                                                                                                                                                                <  1:290467/251‑3 (MQ=255)                                       gTCAGAAGATGAGGCAGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCCGGTAATTTTGGGTCATGGCAACGCGAGACTCGAGTGGGAAGGCGGGCATAACCATCACGCCACCACCCAGGATCACCGCATAGGGATATACCCGATTAATGCTGGTGGCCATAGCCCCGCCCACGTTTTCAAGCCAACTCTAGACGGCAGAGGCGTTTTGAGCATGGACTGAAAA‑AT‑GCGGCAATGGGTAAtttt                                                                                                                                                                                               >  2:496379/1‑250 (MQ=255)                                              gaTGAGGCAGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGAGGCGTTTTCCGCATGGAC‑GAAAAGATCGCG‑CAATGGGGAATTTCGGGGCtg                                                                                                                                                                                        >  1:115613/1‑251 (MQ=255)                                                tGAGGCAGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGGCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGGTCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGGTTTCAAGCAGGCTCTGGACGAAAGAGGAGTTTTCCGCAAGGGA‑GAAAAGATCGCG‑CAATGGGTAATTTAGGGGAtgtt                                                                                                                                                                                      >  2:334974/1‑251 (MQ=255)                                                   ggcagcAATAAAGCGCACGACCGGATGCGGCAGTGGGCGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGAGGCGTTTTCCGCATGGAC‑GAAAAGATCGCG‑CAATGGGTAATTTCGGGGCTgttgtt                                                                                                                                                                                   <  2:81667/247‑1 (MQ=255)                                                          aTAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGAGGCGTTTTCCGCATGGAC‑GAAAAGATCGCG‑CAATGGGTAATTTCGGGGCTGTTGTTCGTACCa                                                                                                                                                                            <  2:371638/251‑1 (MQ=255)                                                            aaaGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGAGGCGTTTTCCGCATGGAC‑GAAAAGATCGCG‑CAATGGGTAATTTCGGGGCTGTTGTTCGTACCAg                                                                                                                                                                           <  1:385498/250‑1 (MQ=255)                                                            aaaGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGAGGCGTTTTCCGCATGGAC‑GAAAAGATCGCG‑CAATGGGTAATTTCGGGGCTGTTGTTCGTACCAg                                                                                                                                                                           <  2:234447/250‑1 (MQ=255)                                                                cgccagtacTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGGCTCGCGGGGGAAGGCGGGCCTACCCATCACGCCACCGCCCTGGATCTCCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGAGGCGTTTTCCGCATGGAC‑GAAAAGATCGCG‑CAATGGGTAATTTCGGTGCTGCTGTTCGtccccgcgcct                                                                                                                                                                      <  2:3559/243‑11 (MQ=255)                                                                    cGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGAGGCGTTTTCCGCATGGAC‑GAAAAGATCGCG‑CAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAgc                                                                                                                                                                  <  2:236354/251‑1 (MQ=255)                                                                          gATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGAGGCGTTTTCCGCATGGAC‑GAAAAGATCTCG‑CAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCAt                                                                                                                                                             <  1:187140/250‑1 (MQ=255)                                                                                  aGTGGACGGCGCAGGTACTTTTGGGGCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCCTATCCATCACGCCACCGCCCAGGATCACCGCCTCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGAGGCGTTTTCCGCATGGCC‑GAAAAGCTCGCG‑CAATGGGGAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGcc                                                                                                                                                    >  2:20871/1‑250 (MQ=255)                                                                                         ggggcAGGTACTTTTGGGTCATGGCAACGTGAGTCGCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGGTTTCAAGCAGACTCTGGACGAAAGAGGCGTTTTCCGCATGGAC‑GAAAAGATCGCG‑CAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGtt                                                                                                                                             <  1:325266/248‑1 (MQ=255)                                                                                              aGGTACTTTGGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGAGGCGTTTTCCGCATGGAC‑GAAAAGATCGCG‑CAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAg                                                                                                                                        <  2:115613/251‑1 (MQ=255)                                                                                                  aCTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGTGCATATCCATCACGCCACCGCCCAGGATCACCGCAGCGGGATCGAACAGATTAATGCTGGTGGCAAGGGCCCGTTTCGCGTTTTCAAGCAGACTCTGGACGAAAGAGGCGTTTTCCGCATGGAC‑GAAAAGATCGCG‑CAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCAc                                                                                                                                    <  1:334974/251‑1 (MQ=255)                                                                                                  aCTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGAGGCGTTTTCCGCATGGAC‑GAAAAGAt                                                                                                                                                                                                                  <  2:28599/174‑1 (MQ=255)                                                                                                  aCTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGAGGCGTTTTCCGCATGGAC‑GAAAAGAt                                                                                                                                                                                                                  >  1:28599/1‑174 (MQ=255)                                                                                                     tttGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGAGGCGTTTTCCGCATGGAC‑GAAAAGATCGCG‑CATTGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAACGCCATTCAAGAGCAATTGGTTTCCAGGCAcccc                                                                                                                                 >  1:419949/1‑250 (MQ=255)                                                                                                                     gagaGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGAGGCGTTTTCCGCATGGAC‑GAAAAGATCGCG‑CAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACgcgc                                                                                                                 >  1:164733/1‑251 (MQ=255)                                                                                                                        agTCTCGTGTGGGAAGGCGTGCATAGCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAAGGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGAGGCGTTTTCCGCATGGAC‑GAAAAGATCGCG‑CAATGGGTAATTTCGGGGCTGTTTTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCA‑gt                                                                                                             <  1:498076/251‑1 (MQ=255)                                                                                                                             cgcgTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGAGGCGTTTTCCGCATGGAC‑GAAAAGATCGCG‑CAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATGCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCA‑GTGTTg                                                                                                         <  2:75499/250‑1 (MQ=255)                                                                                                                                 ggggAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCAACGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGAGGCGTTTTCCGCATGGAC‑GAAAAGATCGCG‑CAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCA‑GTGTTGGGTCa                                                                                                    <  1:9406/250‑1 (MQ=255)                                                                                                                                 ggggAAAGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATTTCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGAGGCGTTTTCAGCATGGAC‑GAACAGATCGCG‑CAATGGGAAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCATGTGCACTTGGTTTCCAGGCACCCAAGATTGCCACACGCGCA‑ggttaggtgcc                                                                                                    >  2:106410/2‑241 (MQ=255)                                                                                                                                      aGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAAAAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGACAGAGGCGTTTTCCGCATGGAC‑GAAAAGATCACG‑CACTGGGTAATTTCGGGGCTGTTGTTAGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCAAGGCACCCAGGATTGCCACCCGAGCA‑GGGTTggggtctatcc                                                                                               >  2:500510/1‑243 (MQ=255)                                                                                                                                         cGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGAGGCGTTTTCCGCATGGAC‑GAAAAGATCGCG‑CAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCA‑GTGTTTGGTCATATCTccc                                                                                            >  1:451037/1‑251 (MQ=255)                                                                                                                                          gggaatatCCAGCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGAGTAAGGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGAGGCGTTGTCCGCATGGAC‑GAAAAGATCGCG‑CAATGGGTAAGTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTGCCAGGCACCCAGGATTGCCACACGCGCA‑GTGTTGGGTCATATCTCCCa                                                                                           <  2:38054/247‑1 (MQ=255)                                                                                                                                                    aTCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGAGGCGTTTTCCGCATGGAC‑GAAAAGATCGCG‑CAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCA‑GTGTTGGGTCATATCTCCCAGGGGGATATg                                                                                 >  1:410134/1‑251 (MQ=255)                                                                                                                                                                  cAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGAGGCGTTTTCCGCATGGAC‑GAAAAGATCGCG‑CAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCA‑GTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTg                                                                   <  1:88412/251‑1 (MQ=255)                                                                                                                                                                       tCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGAGGCGTTTTCCGCATGGAC‑GAAAAGATCGCG‑CAATGGGGAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTACCACACGCGCA‑GTGTTGGGTCATATATCCCAGGGGGATATGACCCAGTTCGCCTGCcac                                                               >  1:14181/1‑250 (MQ=255)                                                                                                                                                                              aTCGGGATCGAATATATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGAGGTGTTTTCCGCATGGAC‑GAAAAGATCGCG‑CAATGGTTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCA‑GTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGtgtg                                                       <  1:391979/251‑1 (MQ=255)                                                                                                                                                                                cGGGATCGAACAGATTACTGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTTGACGAAAGAGACGGTTTCCGCATGGAC‑GAAAAGATCGCG‑CCATGGGGATTTTCGGGGCTGTTGTTCGGACCAGCGTCTTAGCGCCCTTCCAGCGCAATTGGTTTCCAAGCAACCAAGAGTTCCACAC‑CCCACGTGGTGGGACATTTCTACCAGTGGGATATGTACCAGTTCGCCTGcacccccggtagcc                                                     >  2:260964/1‑238 (MQ=255)                                                                                                                                                                                  ggATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGAGGCGTTTTCCGCATGGAC‑GAAAAGATCGCG‑CAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCA‑GTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCAcc                                                   >  1:133771/1‑251 (MQ=255)                                                                                                                                                                                   gATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGAGGCGTTTTCCGCATGGAC‑GAAAAGATCGCG‑CAATGGGTACTTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCA‑GTGTTGGGTCATCTCTCCCAGGGGGATATGACCTAGTTCGCCTGCCACACCgtggaccccc                                                  >  2:340891/1‑244 (MQ=255)                                                                                                                                                                                        aataGATTAAGTCTGGTGGAAATGGCCCTTGCTGCGTTTTCAAGCGTCCTCTGGACGAAAGAGTCTTTTTCCGCATGGAC‑GAAAAGATCGCG‑TAATGGTTAATTTCGGGGCTGTTGTTCGTACCTGCGTCTTAGCGCCTTTCCAGAGAAATTGGTTTCCAGCCACCCAGGATTGCACCACGCGCA‑TTGTTGGTTCATATCTCCCGGGGGGATAGGACCCAGTTCGCCTGCCACACCGTGTGCACCCGt                                                <  1:337025/245‑1 (MQ=255)                                                                                                                                                                                                             aTGGCCCTTGCCGCTTTTTCAAGAGGACTCTGGACGAAAGAGGCGTTTTCCGCATGGAC‑GAAAAGATCGCG‑ATATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTGAGCGCCATTCCAGAGCATTTGTTTGCCAGGCACCCAGGATTGCCACACGTGCA‑GTGTTGGGTTATATCTCCTAGGGGGATATGACCCTGTGCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCacac                        <  1:474483/251‑1 (MQ=255)                                                                                                                                                                                                             aTGGACCGTGACGCGATTTCCAGCAGACTATGGACGAAAGAGGCGTTTTCCGCATGGAC‑GACAAGATCGCG‑CAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGGATTAGCGCCATTCCAGGGCAAATGGTTTCCAGGCCCCCCGGATTTCCACACGCGCA‑GTATTGGGTAATATCTCCCAGGGGGATATGACCCAGTTCGACTGCCACACCGTGTGCACCCGTCCACGGCGAACCGTTCCTCCacac                        >  2:498524/1‑251 (MQ=255)                                                                                                                                                                                                                     tgccgcggtgtaccgCAGACTCTGGACGAAAGAGGCGTTTTCCGCATGGAC‑GAAAAGATAGCG‑CAATGGGTAATGTCGGGGATGTTATTAGTACCCGCGTCATAGCGCCATTCCAGAGCAATTGGAATACAGGCAACCAGGATTGCCACACGCGCA‑GTGTTGGGTCATAGCTCCCAGGGGGAACTGACCCATTTAGCCTGCCCCACCGGGTGCACCCGTCCACGGGTAACCGTTCATCcaccatgataacc                >  2:167138/15‑241 (MQ=255)                                                                                                                                                                                                                          cgTTTTCAAGCAGACTCTGGACGAAAGAGGCGTTTTCCGCATGGAC‑GAAAAGATCGCG‑CAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCA‑GTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATc           >  1:444830/1‑251 (MQ=255)                                                                                                                                                                                                                           gTTTTCAAGCAGACTCTGGACGAAAGAGGCGTTTTCCGCATGGAC‑GAAAAGATCGCG‑CAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCA‑GTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATcc          >  1:186134/1‑251 (MQ=255)                                                                                                                                                                                                                              ttcaagcaaccgctGGACAAAAGAGGCGTTTCCCGCATGGAC‑AAAAAGATCGCG‑AATTGGTTAATTGCGGGGCTGTTTGTCGTACCATCGTCTTAGCGCCATTCCAGAGCAATTGTTTCCCAGGCCCCCGTGTTGGCCCCACGCGCA‑GTTTTGGTTTATATCTCCCGGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATcccc        <  1:69090/238‑1 (MQ=255)                                                                                                                                                                                                                                   gCAGTCTCTGGACTAAAGGGGCTTTTCCCGCAGGGAC‑GAAAAGATGTCG‑CAAGGTGTAATTCCGGGGCTGTTGTGCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGTTTTCCGGGCACCCAGGATTGCCACCCGCGGG‑GTGTTGGGTCATTTCTCCGAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCg  <  1:433647/251‑1 (MQ=255)                                                                                                                                                                                                                                     tcctatcggACAAAAGGGGCTTTTCCCGCATGGAC‑AAAAAGATCGCG‑AAGTGGGTAATTGGGGGGGTTTTTTTCGTCCCCGCGTCTTAGCGCCATTCCAGAGCAATTGGTTCCCAGCCACCAAGGTTCCCCACACGCGCG‑GTGTGGGGTAATACCCCCCGGGGGGATCTGCCCCCGTTCCCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCg  <  2:125616/242‑1 (MQ=255)                                                                                                                                                                                                                                     aGACTCTGGACGAAAGAGGCGTTTTCCGCATGGAC‑GAAAAGATCGCG‑CAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCA‑GTGTTGGGTCATATCTCCCAGGGGGatat                                                                                  <  2:278849/169‑1 (MQ=255)                                                                                                                                                                                                                                     aGACTCTGGACGAAAGAGGCGTTTTCCGCATGGAC‑GAAAAGATCGCG‑CAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCA‑GTGTTGGGTCATATCTCCCAGGGGGatat                                                                                  >  1:278849/1‑169 (MQ=255)                                                                                                                                                                                                                                             gACGAAAGAGGCGTTTTCCGCATGGAC‑GAAAAGATCGCG‑CAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCA‑GTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCg  >  1:125616/1‑241 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                            TGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGAC‑GAAAAGATCGCG‑CAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCA‑GTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCG  >  NC_000913/4306907‑4307383

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑