Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,306,960	G→A	A280A (GCC→GCT)	alsK ←	D‑allose kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,306,960	0	G	A	98.0%	156.5 / NA	49	A280A (GCC→GCT)	alsK	D‑allose kinase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (30/18);  minor base T (1/0);  total (31/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCATTTTATTAGGCATTTGTGCTGAAGAGGGAAAATTACAGTAAAACTATCATTGCAGAAAATAATATGAGTCGTTTTATGTGTTTATTATATATATTGTACCTGTCGTTGGCACAGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGAC‑GAAAAGATCGCG‑CAATGGGTAATTTCGGGGCTGTT  >  NC_000913/4306721‑4307204                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                       ccATGTTATTAGGCAGTTGTGCTGAAGAGGGAAAATTAAAGGAAAACGATCATTGCAGAAAATAATATGAGGTGGGTTATGTGTTTATTATATATATTGTACCTGTCGTTGGCACAGCGGGACATTGCGCTACTGTGTCAACGTTTTACTCATGGGGCTTTAGCTCAGAACTGTGGCAAAAAACGTTGATGCGCCAATACTGCTTCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCAGCAATAAAgcg                                                                                                                                                                                                                                             <  2:411915/251‑1 (MQ=255)  cATTTTATTAGGCATTTGTGCTGAAGAGGGAAAATTACAGTAAAACTATCATTGCAGAAAATAATATGAGTCGTTTTATGTGTTTATTATATATATTGTACCTGTCGTTGGCACAGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCAGCAATAAAgcgc                                                                                                                                                                                                                                            >  1:98919/1‑251 (MQ=255)       tattaGGCATTTGTGCTGAAGAGGGAAAATTACAGTAAAACTATCATTGCAGAAACTAATATGAGGCGTTTTATGAGTTTATTATATATATTGTACCTGTCGTTGGCACAGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCTCCAATATTGCTGCACCCTGAGCGCCATTAACGTCAGAAGAGGAGGCTGCAATAAAGCGCACGAc                                                                                                                                                                                                                                       >  2:69090/1‑251 (MQ=255)              cATTTGTGCTGAAGAGGGAAAATTACAGTAAAACTATCATTGCAGAAAATAATATGAGTCGTTTTATGTGTTTATTATATATATTGTACCTGTCGTTGGCACAGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCAGCAATAAAGCGCACGACCTGATGc                                                                                                                                                                                                                                <  2:136555/251‑1 (MQ=255)                  tgtgCTGAAGAGGGAAAATTACAGTAAAACTATCATTGCAGAAAATAATATGAGTCGTTTTATGTGTTTATTATATATATTGTACCTGTCGTTGGCACAGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCAGCAATAAAGCGCACGACCTGATGCGGc                                                                                                                                                                                                                             >  1:190549/1‑250 (MQ=255)                                 aaaTTACAGTAAAACTATCATTGCAGAAAATAATATGAGTCGTTTTATGTGTTTATTATATATATTGTACCTGTCGTTGGCACAGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCAGCAATAAAGCGCACGCCCTGATGCGGCAGTGGAAGGCGCAggg                                                                                                                                                                                                             >  2:385498/1‑250 (MQ=255)                                      aCAGTAAAACTATCATTGCAGAAAATAATATGAGTCGTTTTATGTGTTTATTATATATATTGTACCTGTCGTTGGCACAGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCAGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACtt                                                                                                                                                                                                         <  1:470250/250‑1 (MQ=255)                                       cAGTAAAACTATCATTGCAGAAAATAATATGAGTCGTTTTATGTGTTTATTATATATATTGTACCTGTCGTTGGCACAGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCAGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGGACtttt                                                                                                                                                                                                       >  2:134647/1‑251 (MQ=255)                                       cAGTAAAACTATCATTGCAGAAAATAATATGAGTCGTTATATGTGTTTATTATATATATTGTACCTGTCGTTGGCACAGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCAGCAATAAAGCGCACGACCTGATGAGGCAGTGGACGGCGCAGGGACtttt                                                                                                                                                                                                       >  1:140117/1‑251 (MQ=255)                                                             aataatATGAGTCGTTTTATGTGTTTATTATATATATTGTACCTGTCGTTGGCACAGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCAGCAATAAAGCGCACGACCTGATGCGGCAGGGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGtctc                                                                                                                                                                                 <  1:290703/251‑1 (MQ=255)                                                                                            atatatTGTACCTGTCGTTGGCACAGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCAGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGTGAAGGCGGGCATATCCATCACGcc                                                                                                                                                   <  1:161648/250‑1 (MQ=255)                                                                                             tatatTGTACCTGTCGTTGGCACAGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCAGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGGCATGGCAACGAGAGGCTCGAGGGGGAAGGCGGGCATATCCATCACGCCAc                                                                                                                                                 >  2:391979/1‑251 (MQ=255)                                                                                               tatTGTACCTGTCGTTGGCACAGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCAGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGACAccc                                                                                                                                               >  1:3559/1‑250 (MQ=255)                                                                                                   gTACCTGTCGTTGGCACAGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCAGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCa                                                                                                                                           <  2:191467/251‑1 (MQ=255)                                                                                                   gTACCTGTCGTTGGCACAGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCAGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCACATCCATCACGCCACCGCCCa                                                                                                                                           <  2:98919/251‑1 (MQ=255)                                                                                                         gTCGTTGGCACAGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCAGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATc                                                                                                                                      >  1:413758/1‑250 (MQ=255)                                                                                                                gCACAGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCAGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGGTCACCGcctc                                                                                                                              >  2:140591/1‑248 (MQ=255)                                                                                                                  acaGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCAGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCgg                                                                                                                            >  1:75499/1‑251 (MQ=255)                                                                                                                  acaGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCAGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCgg                                                                                                                            >  1:371638/1‑251 (MQ=255)                                                                                                                     gCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCAGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGAt                                                                                                                         >  1:105468/1‑251 (MQ=255)                                                                                                                       gtgACACTGCGCGACTGCGCCAACGTTTTACTCATTGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGAAGCGTCAATATTGCTGCGCCCTGAGCGCCATTAAAGTCAGAAGATGAGGCAGCAATAAAGCGCACGACCTGATGCGGCAGTGGACTGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGTGCATATCCATCACGCCCCCGCCCAGGATCACCGCATCTGGagcg                                                                                                                       <  2:20097/249‑4 (MQ=255)                                                                                                                           cATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCAGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAgg                                                                                                                                         >  1:21227/1‑229 (MQ=255)                                                                                                                           cATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCAGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGGCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAgg                                                                                                                                         <  2:21227/229‑1 (MQ=255)                                                                                                                                       gtgCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCAGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCtgg                                                                                                       >  1:429391/1‑251 (MQ=255)                                                                                                                                           cAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCAGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAAAGCTGGTGGc                                                                                                   >  2:353749/1‑251 (MQ=255)                                                                                                                                                     cTCATGGGGCTTTAGCACAGAACTGTGGCAACAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTACAGTCAGAAGATGAGGCAGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCgc                                                                                         >  1:197338/1‑250 (MQ=255)                                                                                                                                                        aTGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTACAGTCAGAAGATGAGGCAGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGcc                                                                                      >  1:218142/1‑251 (MQ=255)                                                                                                                                                                   gCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCAGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAg                                                                           <  1:140591/251‑1 (MQ=255)                                                                                                                                                                      caGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCAGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGc                                                                          >  1:331018/1‑249 (MQ=255)                                                                                                                                                                       aGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCAGCAATAAAGCGCACGACCTGCTGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGGTTTCAAGc                                                                          >  1:120072/1‑248 (MQ=255)                                                                                                                                                                       aGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCAGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCg                                                                                                                       >  1:449345/1‑203 (MQ=255)                                                                                                                                                                       aGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCAGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCg                                                                                                                       <  2:449345/203‑1 (MQ=255)                                                                                                                                                                               ggCAAAAAACGTTGATGCGCCAATATTGCTGCAGCCTGAGCGCCATTAAAGTCAGAAGATGAGGCAGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGAAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGAc                                                               <  1:506254/251‑1 (MQ=255)                                                                                                                                                                                    aaaaCGTTGATGCGCCAATATTGCTGCTCCCTGAGCGCCATTAAAGTCAGAAGATGAGGCAGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAg                                                          <  1:183306/251‑1 (MQ=255)                                                                                                                                                                                           tGATGCGCCAATATTTCTGCACCCTGAGCGCCATTACAGTCAGAAGATGAGGCAGCAATAACGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGCGAGACTCGCGTGGGAAGGCGGGCATATCCATCACGCCAACGCCCAGGATCACCGCCACGGGATCGAACAGCTTAATGCTGGTGGAAAGGGCCCGTGCCGCGCTTTCAAGCAAACTATGGACGAAAgagagaat                                                   >  2:145517/1‑244 (MQ=255)                                                                                                                                                                                           tGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCAGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGTAGACTCTGGACGAAAGAGGCGtt                                                   >  1:394053/1‑251 (MQ=255)                                                                                                                                                                                             aTGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCAGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGAGGCGtttt                                                 <  1:134647/251‑1 (MQ=255)                                                                                                                                                                                               gcgcCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCAGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGAGGCGTTTTcc                                               <  2:190549/251‑1 (MQ=255)                                                                                                                                                                                                    aaTATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCAGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGAGGCGTTTTCCGCATg                                          >  1:243282/1‑251 (MQ=255)                                                                                                                                                                                                       atTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCAGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGAGGCGTTTTCCGCATGGAc                                       >  1:38054/1‑251 (MQ=255)                                                                                                                                                                                                         tgctgcACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCAGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGCAGGCGGGCATATCCATCAAGCCACCGCCCAGGATCAACGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGAGGCGTTTTCCGCATggc                                        >  1:463238/1‑247 (MQ=255)                                                                                                                                                                                                                  cTGAGCGCCATTAAAGACAGAAGATGCGGCAGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCCCGCCACCGCCCAGGATCACCGCATCGGGGAAGAACAGATTAATGCTGGTGGCAATGGCCCGCGCCGCGTTTTCAAGCAGACTCTGGACGAAAGAGGCGTTTTCCCCATGTAC‑GGAAAGAAcgc                           >  2:383078/1‑251 (MQ=255)                                                                                                                                                                                                                       cgcCATTAAAGACAGAAGATGAGGCAGCAATACAGCGCACGACCTGATGCGGAAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGAGGCGATTTCCGCATGGAC‑GAAAAGATCGCG‑CAAt                     >  1:190774/1‑251 (MQ=255)                                                                                                                                                                                                                           aTTAAAGTAAGAAGATGAGGCAGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGAGGCGTTTTCCGCATGGAC‑GAAAAGATCGCG‑CAATGGGt                 <  2:264307/251‑1 (MQ=255)                                                                                                                                                                                                                             tAAAGTCAGAAGATGAGGCAGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGAGGCGTTTTCCGCATGGAC‑GAAAAGATCGCG‑CAATGGGTa                <  1:253185/250‑1 (MQ=255)                                                                                                                                                                                                                                aGTCAGAAGATGAGGCAGCAGTAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGATAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCTCCGCAGCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAATCAGTCTCTGGACGAAAGAGGCGTTTTCCGCATGGAC‑GAAAAGATCGCG‑CAATGGGTAAtgt            <  1:290467/251‑3 (MQ=255)                                                                                                                                                                                                                                 gTCAGAAGATGAGGCAGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCCGGTAATTTTGGGTCATGGCAACGCGAGACTCGAGTGGGAAGGCGGGCATAACCATCACGCCACCACCCAGGATCACCGCATAGGGATATACCCGATTAATGCTGGTGGCCATAGCCCCGCCCACGTTTTCAAGCCAACTCTAGACGGCAGAGGCGTTTTGAGCATGGACTGAAAA‑AT‑GCGGCAATGGGTAAtttt           >  2:496379/1‑250 (MQ=255)                                                                                                                                                                                                                                        gaTGAGGCAGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGAGGCGTTTTCCGCATGGAC‑GAAAAGATCGCG‑CAATGGGGAATTTCGGGGCtg    >  1:115613/1‑251 (MQ=255)                                                                                                                                                                                                                                          tGAGGCAGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGGCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGGTCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGGTTTCAAGCAGGCTCTGGACGAAAGAGGAGTTTTCCGCAAGGGA‑GAAAAGATCGCG‑CAATGGGTAATTTAGGGGAtgtt  >  2:334974/1‑251 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                       CCATTTTATTAGGCATTTGTGCTGAAGAGGGAAAATTACAGTAAAACTATCATTGCAGAAAATAATATGAGTCGTTTTATGTGTTTATTATATATATTGTACCTGTCGTTGGCACAGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGAC‑GAAAAGATCGCG‑CAATGGGTAATTTCGGGGCTGTT  >  NC_000913/4306721‑4307204

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑