Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,306,035	C→T	G188D (GGC→GAC)	yjcS ←	linear primary‑alkylsulfatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,306,035	0	C	T	97.4%	112.2 / NA	39	G188D (GGC→GAC)	yjcS	linear primary‑alkylsulfatase
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (22/15);  total (23/16)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTGACGATAGTTTTCGTCGGTGCAATAATGCTCGGGTCGCCCGTTGCCAGCGTCACGCCAAGGCCATTACCCACATTGCCTTGCGCGTTGTGCGGCAGTAACAGACCGTAAGAGTAGAGCGCACGGCGGCTCATGATGTTACCCGCCAGCACGTTTTCGCTGATGGCTTCGTCCATAAAGCCTGCAGGGGCAATCACCTGAACTTTGCCGGATTTAACATCGGCTTCAGAGATAATGCCTTTCACG‑CCACCATAGTGGTCGGTGTG‑GCTGTGAGTGTAGATAACGGC‑AACAATCGGTTTTTGCGGACGATGCTGGAAGTAAAGGTCAAGTGCGGCTTTTGCGGCAGGCGGCGTCACCAGCGGGTCGATAACAATAATGCCTTTCTCGCCCTCAACGAACGTAATGTTAGAGATATCCTGGCCGCGCACCTGATACATTTTATCGGTGACTTTGAACAGGCCAGAAATACCGTTGATTTGCGACTGACGCCAC  >  NC_000913/4305789‑4306280                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                        cggccGATATTTTTCGTGGGGGAAATAAGGCCCGGGCCGCGCGTGGCCAGCGCCACGCAAAGGCCATTACCCACATGGCCTTGCGTGTTGTGCGGCAGTAACAGCCCGTAAGAGAGGAGCGCTGGGCGGCTCATGTTGTTACCCGCCAGCACGTTTTCGCTGATGGCTTCGTCCATAAAGCCTGCAGGGGCAATCACCTGAACTTTGCCGGATTTAACATCGGCTTCAGAGATAATGCCTTTCACG‑tcacc                                                                                                                                                                                                                                                     <  2:136645/247‑1 (MQ=255)              tcttcgGTTCAATAAGGCTCGGGTCGCCCGGTGCCAGCTTCCCGCCAGGGCCATTACCCACATTGCCTTGCGCTTTGTGCGGCAGTAACATACCGTAAGAGTAGAGCGCACGGGTGCTCATGATGTTACCCGCCAGCACGTTTTCGCTGATGGCTTCGTCCATAAAGCCTGCAGGGGCAATCACCTGAACTTTGCCGGATTTAACATCGGCTTCAGAGATAATGCCTTTCACG‑TCACCATAGTGGTCGgt                                                                                                                                                                                                                                         <  1:290926/247‑1 (MQ=255)                gtcgGTGCAATAATGCTCGGGTCGCCCGTTGCCAGCGTCACGCCAAGGCCATTACCCACATTGCCTTGCGCGTTGTGCGGCAGTAACAGACCGTAAGAGTAGAGCGCACGGCGGCTCATGATGTTACCCGCCAGCACGTTTTCGCTGATGGCTTCGTCCCTAAAGCCTGCAGGGGCAATCACCTGAACTTTGCCGGATTTAACATCGGCTTCAGAGATAATGCCTTTCCCG‑TCCCCCTAGTGGTCGGtgtg                                                                                                                                                                                                                                      >  2:164156/1‑251 (MQ=255)                         ataatGCTCGGGTCGCCCGTTGCCAGCGTCACGCCAAGGCCATTACCCACATTGCCTTGCGCGTTGTGCGGCAGTAACAGACCGTAAGAGTAGAGCGCACGGCGGCTCATGATGTTACCCGCCAGCACGTTTTAGCTGATGGCTTCGTCCATAAAGCCTGCAGGGGCAATCACCTGAACTTTGCCGGATTTAACATCGGCTTCAGAGATAATGCCTTTCACG‑TCACCATAGTGGTCGGTGTG‑GCTGTGAgt                                                                                                                                                                                                                            >  1:345740/1‑251 (MQ=255)                               cTCGGGTCGCCCGTTGCCAGCGTCACGCCAAGGCCATTACCCACATTGCCTTGCGCGTTGTGCGGCAGTAACAGACCGTAAGAGTAGAGCGCACGGCGGCTCATGATGTTACCCGCCCGCACGTTTTCGCTGATGGCTTCGTCCATAAAGCCTGCAGGGGCAATCACCTGAACTTTGCCGGATTTAACATCGACTTCAGAGATAATGCCTTTCACG‑TCACCATAGTGGTCGGTGGG‑GCtgggatagtaga                                                                                                                                                                                                                       >  2:273086/1‑240 (MQ=255)                               cTCGGGTCGCCCGTTGCCAGCGTCACGCCAAGGCCATTACCCACATTGCCTTGCGCGTTGTGCGGCAGTAACAGACCGTAAGAGTAGAGCGCACGGCGGCTCATGATGTTACCCGCCAGCACGTTTTCGCTGATGGCTTCGTCCATAAAGCCTGCAGGGGCAATCACCTGAACTTTGCCGGATTTAACATCGGCTTCAGAGATAATGCCTTTCACG‑TCACCATAGTGGTCGGTGTG‑GCTGTGAgggt                                                                                                                                                                                                                          >  1:299356/1‑244 (MQ=255)                                    gtcccccGTTCCCCGCGTCCCGCCATGCCCTTTCCCCACTTTGCCTTCCCCTTTTTGCGGCTGTACCAGTCCGTAAGATTAGCGCGCCCGGCTGCTCTTGTTGTTCCCCGCCCGCACGTTTTCGCTGATGGCTTCGTCCATAAAGCCTGCAGGGGCAATCACCTGAACTTTGCCGGATTTAACATCGGCTTCAGAGATAATGCCTTTCACG‑TCACCATAGTGGTCGGTGTG‑GCTGTGAGTGTAGATAACgg                                                                                                                                                                                                                 <  1:406234/247‑1 (MQ=255)                                        cccGTTGCCAGCGTCACGCCAAGGCCATTACCCACATTGCCTTGCGCGTTGTGCGGCAGTAACAGACCGTAAGAGTAGAGCGCACGGCGGCTCATGATGTTACCCGCCAGCACGTTTTCGCTGATGGCTTCGTCCATAAAGCCTGCAGGGGCAATCACCTGAACTTTGCCGGATTTAACATCGGCTTCAGAGATAATGCCTTTCACG‑TCACCATAGTGGTCGGTGTG‑GCTGTGAGTGTAGATAACGGc‑aac                                                                                                                                                                                                            >  1:394633/1‑251 (MQ=255)                                            gtgccgtcttcgcgcgctgcccATTACCCCAATTGCTTTGCGCGTTGTGCGGCAGAAACATACCGTAAGAGTAGAGCGCACGGCGGTGTATGAGGTTCCCCGCCAGCACGTTTTCGCTGATGGCTTCGTCCATAAAGCCTGCAGGGGCAATCACCTGAACTTTGCCGGATTTAACATCGGCTTCAGAGATAATGCCTTTCACG‑TCACCATAGTGGTCGGTGTG‑GCTGTGAGTGTAGATAACGGC‑AACAATc                                                                                                                                                                                                        <  1:122393/231‑1 (MQ=255)                                                 aGCGTCACGCCAAAGCCATTACCCACATTGCCTTGCGCGTTGTGCGGCAGTAACAGACCGTAAGAGTAGAGCGCACGGCGGCTCATGATGTTACCCGCCAGCACGTTTTCGCTGATGGCTTCGTCCATAAAGCCTGCAGGGGCAATCACCTGAACTTTGCCGGATTTAACATCGGCTTCAGAGATAATGCCTTTCACG‑TCACCATAGTGGTCGGTGTG‑GCTGTGAGTGTAGATCACGGC‑GACACTCGGttt                                                                                                                                                                                                   >  2:252804/1‑251 (MQ=255)                                                    gTCACGCCAAGGCCATTACCCACATTGCCTTGCGCGTTGTGCGGCAGTAACAGACCGTAAGAGTAGAGCGCACGGCGGCTCATGATGTTACCCGCCAGCACGTTTTCGCTGATGGCTTCGTCCATAAAGCCTGCAGGGGCAATCACCTGAACTTTGCCGGATTTAACATCGGCTTCAGAGATAATGCCTTTCACG‑TCACCATAGTTGTCGGTGTG‑GCTGTGAGTGTAGATACAGGC‑AACAATCGGTTTTTg                                                                                                                                                                                                >  1:75912/1‑251 (MQ=255)                                                    gTCACGCCAAGGCCATTACCCACATTGCCTTGCGCGTTGTGCGGCAGTAACAGACCGTAAGAGTAGAGCGCACGGCGGCTCATGATGTTACCCGCCAGCACGTTTTCGCTGATGGCTTCGTCCATAAAGCCTGCAGGGGCAATCACCTGAACTTTGCCGGATTTAACATCGGCTTCAGAGATAATGCCTTTCACG‑TCACCATAGTGGTCGGTGTG‑GCTGTGAGTGTAGATAACGGC‑AACAATCGGTTTTTg                                                                                                                                                                                                >  1:14472/1‑251 (MQ=255)                                                         gccgaGGCCATTACCCACATTGCCTTGCGCGTTGAGCGACAGAAACTGACCGTAAGCGTAGAGCGCACGGCGGCACATACTGTTACCCGCAAGCTAGGTTTCGCTGCAGTCTTCGCCCATAAAGCCTGCAGGGGCCATCACCTGAACTTTGCCGGATTTAACATCGGCTTCAGAGATAATGCCATTCACGTCCAC‑ATAGTAGTCGGTGTG‑GCTGGGAGGGTAGATAACGGCCAACA‑TCGGttttttcgttc                                                                                                                                                                                           >  2:65744/5‑245 (MQ=255)                                                         gCCAAGGCCATTACCCACATTGCCTTGCGCGTTGTGCGGCAGTAACAGACCGTAAGAGTAGAGCGCACGGCGGCTCATGATGTTACCCGCCAGCACGTTTTCGCTGATGGCTTCGTCCATAAAGCCTGCAGGGGCACTCACCTGAACTTTGCCGGATTTAACATCGGCTTCAGAGATAATGCCTTTCACG‑TCACCATAGTGGACGGGGTG‑GCTGTGAGTGGAGATAACGGC‑AACAATCGGTTTTTTCgg                                                                                                                                                                                             >  2:503538/1‑249 (MQ=255)                                                                ccATTACCCACATTGCCTTGCGCGTTGTGCGGCAGTAACAGACCGTAAGAGTAGAGCGCACGGCGGCTCATGATGTTACCCGCCAGCACGTTTTCGCTGATGGCTTCGTCCATAAAGCCTGCAGGGGCAATCACCTGAACTTTGCCGGATTTAACATCGGCTTCAGAGATAATGCCTTTCACG‑TCACCATAGTGGTCGGTGTG‑GCTGTGAGTGTAGATAACGGC‑AACAATCGGTTTTTGCGGACGATGCTg                                                                                                                                                                                    >  1:54297/1‑251 (MQ=255)                                                                                       gTTGTGCGGCAGTAACAGACCGTAAGAGTAGGGCGCACGGCGGCTAATGATGTTACACGCCAGCACGTTTTCGCTGATGGCTTCGTCCATAAAGCCTGCAGGGACAATCACCTGAACTTTGCCGGATTTAACATCGGCTTCAGCGTTAATGCCTTTCACG‑TCACCTTAGTGGTCGGTGGG‑GAAGTGAGTGTAGATAACGGC‑ATCAATCGGTTTTTGCGGACAATGCTGGaatgaaaggaccagagcggcct                                                                                                                                                             >  2:112882/1‑231 (MQ=255)                                                                                       gTTGTGCGGAAGAAACAGACCGTAAGAGTAGAGCGCACGGCGGCTAATGATTTGACCCGCCAGCACGTTTTCGCTGATGGCTTCGTCCATAAAGCCTGCAGGGGCAATCACCTGAACTTTGCCGGATTTAACATCGGCTTCAGAGATAATGCCTTTCACG‑TCACCATAGTGGTCGGTGTG‑GCTGTGAGTGTAGATAACGGC‑AACAATCGGTTTTTGCGGACGATGCTGGAAGTAAAGGTCAAGTGCGGCtt                                                                                                                                                             <  1:157310/251‑1 (MQ=255)                                                                                              ggCAGTAACAGACCGTAAGAGTAGAGCGCACGGCGGCTCATGATGTTACCCGCCAGCACGTTTTCGCTGATGGCTTCGTCCATAAAGCCTGCAGGGGCAATCACCTGAACTTTGCCGGATTTAACATCGGCTTCAGAGATAATGCCTTTCACG‑TCACCATAGTGGTCGGTGTG‑GCTGTGAGGGTAGATAACGGC‑AACAATCGGTTTTTGCGGGCGATGCTGGAAGGAAAGGGCAAGGGCGGCTTTTGCggg                                                                                                                                                      >  2:314215/1‑250 (MQ=255)                                                                                                 aGTAACAGACCGTAAGAGTAGAGCGCACGGCGGCTCATGATGTTACCCGCCAGCACGTTTTCGCTGATGGCTTCGTCCATAAAGCCTGCAGGGGCAATCACCTGAACTTTGCCGGATTTAACATCGGCTTCAGAGATAATGCCTTTCACG‑TCACCATAGTGGTCGGTGTG‑GCTGTGAGTGTAGATAACGGC‑AACAATCGGttt                                                                                                                                                                                                   >  1:278134/1‑203 (MQ=255)                                                                                                              aagggtggaggccccgccggcTCATGATGTTACCCGCCAGCACGTTTTCGCTGATGGCTTTGTCCATAAAGCCTGCAGGGGCAATCACCTGAACTTTGCCGGATTTAACATCGGCTTCAGAGATAATGCCTTTCACG‑TCACCATAGTGGTCGGTGTG‑GCTGTGAGTGTAGATAACGGC‑AACAATCGGTTTTTGCGGACGATGCTGGAAGTAAAGGTCAAGTGCGGCTTTTGCGGCAGGCGGCGTCACCAGc                                                                                                                                      <  1:267355/234‑1 (MQ=255)                                                                                                              aaGAGTAGAGCGCACGGGGGCTCATGATTTTCCCCGCCAGCACGTTTTCGCTGATGGCTTCGTCCATAAAGCCTGCAGGGGCAATCACCTGAACTTTGCCGGATTTAACATCGGCTTCAGAGATAATGCCTTTCACG‑TCACCATAGTGGTCGGTGTG‑GCTGTGAGTGTAGATAACGGC‑AACAATCGGTTTTTGCGGACGATGCTGGAAGTAAAGGTCAAGTGCGGCTTTTGCGGCAGGCGGCGTCACCAGc                                                                                                                                      <  1:360741/251‑1 (MQ=255)                                                                                                                                ggcTCATGATGTTACCCGCCAGCACGTTTTCGCTGATGGCTTCGTCCATAAAGCCTGCAGGGGCAATCACCTGAACTTTGCCGGATTTAACATCGGCTTCAGAGATAATGCCTTTCACG‑TCACCATAGTGGTCGGTGTG‑GCTGTGAGTGTAGATAACGGC‑AACAATCGGATTTTGCGGACGATGCTGGAAGTAAAGGTCACGTGCGGCTTTTGCGGCAGGCGGAGTCACCAACGGGTCGATAACaataaat                                                                                                                    >  1:274152/1‑249 (MQ=255)                                                                                                                                 gcTCATGATGTTACCCGCCAGCACGTTTTCGCTGATGGCTTCGTCCATAAAGCCTGCAGGGGCAATCACCTGAACTTTGCCGGATTTAACATCGGCTTCAGAGATAATGCCTTTCACG‑TCACCATAGTGGTCGGTGTG‑GCTGTGAGTGTAGATAACGGC‑AACAATCGGTTTTTGCGGACGCTGCTGGAAGTAAAGGTCAAGTGCGGCTTTTGCGGCAGGCGGCGGCACCAGCGGGTCGATAACAAAAATg                                                                                                                    >  1:274523/1‑250 (MQ=255)                                                                                                                                     atgatgTTACCCGCCAGCACGTTTTCGCTGATGGCTTCGTCCATAAAGCCTGCAGGGGCAATCACCTGAACTTTGCCGGATTTAACATCGGCTTCAGAGATAATGCCTTTCACG‑TCACCATAGTGGTCGGTGTG‑GCTGTGAGTGTAGATAACGGC‑AACAATCGGTTTTTGCGGACGATGCTGGAAGTAAAGGTCAAGTGCGGCTTTTGCGGCAGGCGGCGGCACCAGCGGGTCGGTAAAaataaatccttt                                                                                                               >  1:19291/1‑244 (MQ=255)                                                                                                                                                 gCCAGCCCTTTTTCGCTTATGGCTTCGTCGAGAAAGCCTGCAGGGGCAATCACCTGAACGTTGCCGGTTTTAACACCGGCTTCAGAGAGTATGCTTTTCACG‑TCACCATAGTGGTTGGTGTG‑GCTGTGAGTGTAGATAACGGC‑AACAAGCGGTGTTTGCGGACGATGCTGGAAGTAAATGTCAAGTGCGGCTTTTGCGGCAGGCGGCGTCACCAGCGGGTCGATAACAATAATGCCTTTCTCGCCCTCaac                                                                                                   <  2:390664/251‑1 (MQ=255)                                                                                                                                                      cACGTTTTCGCTGATGGCTTCGTCCATAAAGCCTGCAGGGGCAATCACCTGAACTTTGCCGGATTTAACATCGGCTTCAGAGATAATGCCTTTCACG‑TCACCATAGTGGTCGGTGTG‑GCTGTGAGTGTAGATAACGGC‑AACAATCGGTTTTTGCGGACGATGCTGGAAGGAAAGGTCAAGTGCGGCTTTTGCGGCAGGGGGCGTCACCACGGGGGCGATAACAAAAATGCCCTTTTCTCCCTCaccgaacg                                                                                              >  2:1417/1‑251 (MQ=255)                                                                                                                                                          ttttCGCTGATGGCTTCGTCCATAAAGCCTGCAGGGGCAATCACCTGAACTTTGCCGGATTTAACATCGGCTTCAGAGATAATGCCTTTCACG‑TCACCATAGTGGTCGGTGTG‑GCTGTGAGTGTAGATAACGGC‑AACAATCGGTTTTTGCGGACGATGCTGGAAGTAAAGGTCAAGTGCGGCTTTTGCGGCAGGCGGCGTCACCAGCGGGGCGATAACAATAATTCCTTTCTCGCCCTCAACGAACGTAAt                                                                                          >  1:225662/1‑251 (MQ=255)                                                                                                                                                             tCGCTGATGGCTTCGTCCATAAAGCCTGCAGGGGCAATCACCTGAACTTTGCCGGATTTAACATCGGCTTCAGAGATAATGCCTTTCACG‑TCACCATAGTGGTCGGTGTG‑GCTGTGAGTGTAGATAACGGC‑AACAATCGGTTTTTGCGGACGATGCTGGAAGTAAAGGTCAAGTGCGGCTTTTGCGGCAGGCGGCGTCACCAGCGGGTCGATAACAATAATGCCTTTCTCGCCCTCAACGAACGTAATGt                                                                                        <  1:164156/250‑1 (MQ=255)                                                                                                                                                               gCTGATGGCTTCGTCCATAAAGCCTGCAGGGGCAATCACCTGAACTTTGCCGGATTTAACATCGGCTTCAGAGATAATGCCTTTCACG‑TCACCATAGTGGTCGGTGTG‑GCTGTGAGTGTAGATAACGGC‑AACAATCGGTTTTTGCGGACGATGCTGGAAGTAAAGGTCAAGTGCGGCTTTTGCGGCAGGCGGCGTCACCAGCGGGTCGATAACAATAATGCCTTTCTCGCCCTCAACGAACGTAATGTTag                                                                                     <  1:314215/251‑1 (MQ=255)                                                                                                                                                                  gATGGCTTCGGTCATAAAGCCGGCAGGGGAAATCACCTGAACTTTGCCGGAGTTAACCGCGGCTTCAGAGATAATGCCTTTCACG‑GCACCATAGTGGTCGGTGTG‑GCTGTGAGTGTAGATAACGGC‑AACAATCGGTTTTTGCGGACGATGCTGGAAGTAAAGGTCAAGTGCGGCTGTTGCGGCAGGCGGCGTGACCAGCGGGTCGATAACAATAATGCCTTTCTCGCCCTCAACGAACGTAATGTTAgaga                                                                                  <  2:64608/251‑1 (MQ=255)                                                                                                                                                                           gTCCATAAAGCCTGCAGGGGCAATCACCTGAACTTTGCCGGATTTAACATCGGCTTCAGAGATAATGCCTTTCACG‑TCACCATAGTGGTCGGTGTG‑GCTGTGAGTGTAGATAACGGC‑AACAATCGGTTTTTGCGGACGATGCTGGAAGTAAAGGTCAAGTGCGGCTTTTGCGGCAGGCGGCGTCACCAGCGGGTCGATAACAATAATGCCTTTCTCGCCCTCAACGAACGTAATGTTAGAGATATCCTGGc                                                                         <  1:503538/251‑1 (MQ=255)                                                                                                                                                                               aTAAAGCCTGCAGGGGCAATCACCTGAACTTTGCCTGATTTAACATCGGCTTCAGAGATAATGCCTTTCACG‑TCACCATAGTGGTCTGTGTG‑GCTGTGAGTGTAGATAACGGC‑AACAATCGGTTTTTGCGGACGATGCTGGAAGTAAAGGTCAAGTGCGGCTTTTGCGGCAGGCGGCGTCACCAGCGGGTCGATAACAATAATGCCTTTCTCGCCCTCAACGAACGTAATGTTAGAGATATCCTGGCcgcg                                                                     <  1:289870/251‑1 (MQ=255)                                                                                                                                                                                    gCCTGCAGGGGCAATCACCTGAATCTTGCCGGATTTAACATCGGCTTCAGAGATAATGCCTTTCACG‑TCACCATAGTGGTCGGTGTG‑GCTGTGAGTGTAGATAACGGC‑AACAATCGGTTTTTGCGGACGATGCTGGAAGTAAAGGTCAAGTGCGGCTTTTGCGGCAGGCGGCGTCACCAGCGGGTCGATAACAATAATGCCTTTCTCGCCCTCAACGAACGTAATGTTAGAGATATCCTGGCCGTGCACCt                                                                <  1:223983/251‑1 (MQ=255)                                                                                                                                                                                           ggggCAATCACCTTAACTTTGCCGGATTTAACATCGGCTTCAGAGATAATGCCTTTCACG‑TCACCATAGTGGTCGGTGTG‑GCTGTGAGTGTAGATAACGGC‑AACAATCGGTTTTTGCGGACGATGCTGGAAGTAAAGGTCAAGTGCGGCTTTTGCGGCAGGCGGCGTCACCAGCGGGTCGATAACAATAATTCCTTTCTCGCCCTCAACGAACGTAATGTTAGAGATATCCTGGCCGCGCACCTGATACAt                                                         <  1:491042/251‑1 (MQ=255)                                                                                                                                                                                               cAATCACCTGAACTTTGCCGGATTTAACATCGGCTTCAGAGATAATGCCTTTCACG‑TCACCATAGTGGTCGGTGTG‑GCTGTGAGTGTAGATAACGGC‑AACAATCGGTTTTTGCGGACGATGCTGGAAGTAAAGGTCAAGTGCGGCTTTTGCGGCAGGCGGCGTCACCAGCGGGTCGCTAACAATAATGACTTTCTCCCCCTCAACGAACGTAATGTTAGGAATAACCTGGCCGCGCCCCTGCTACAtttt                                                      >  1:366331/1‑250 (MQ=255)                                                                                                                                                                                                            tttGCCGGATTTAACATCGGCTTCAGAGATAATGCCTTTCACG‑TCACCATAGTGGTCGGAGTG‑GCTGTGAGTGTAGATAACGGC‑AACAATCGGTTTTTGCGGGCGATGCTGGACGTAAAGGACAAGTGCGGATTTTGCGGCAGGCGGCGTAAACAGCGGGTAGATAACAATAATGACTTTCTCGCCCTAAAAGGACGCCATGGTATAGATATTCTTGACGCGCAACCGGTACATTTTATCGGGGCCTTTg                                         >  1:466591/1‑250 (MQ=255)                                                                                                                                                                                                                             cGGCTTCAGAGATAATGCCTTTCACG‑TCACCATAGTGGTCGGTGTG‑GCTGTGAGTGTAGATAACGGC‑AACAATCGGTTTTTGCGGACGATGCTGGAAGTAAAGGTCAAGTGCGGCTTTTGCGGCAGGCGGCGTCACCAGCGGGTCGATAACAATAATGCCTTTCTCGCCCTCAACGAACGTAATGTTAGAGATATCCTGGCCGCGCACCTGATACATTTTATCGGTGACTTTGAACAGGCCAGAAATACCg                       <  1:190445/251‑1 (MQ=255)                                                                                                                                                                                                                                 ttCAGAGATAATGCCTTTCACG‑TCACCATAGTGGTCGGTGTG‑GCTGTGAGTGTAGATAACGGC‑AACAATCGGTTTTTGCGGACGATGATGGAAGTAAAGGTCAAGTGCGGCTTTTGCGGCAGACGGCGTCACCAGCGGATCGAAAACAATCATGCCTTTCGCCCCCTCAACGAGCGTAATGTTAGAGATATACTGGACGCGGACCTGATACATTTTAACGTTGACTTTTAACAGGCCAGAAATAACGTTg                    >  1:215954/1‑250 (MQ=255)                                                                                                                                                                                                                                                   cACG‑TCACCATAGTGGTCGGTGTG‑GCTGTGAGTGTAGATAACGGC‑CACACTCGGTTTTTGCGGACGATGCTGGAAGTAAAGGTCAAGTGCGGCTTTTGCGGCAGGCGGCGGTACCAACGGGTGGGTAACAAATATTCCTTTCTCGCCCTCAACGAAAGTACAATTAGCGATATCCTGGCCGCGCACCCGCTACTTTTTCTCGGTGACTTTGTAAAGGACAGAAATACCGGTGTTTTGTGACTGACGcccc  >  2:361056/1‑248 (MQ=255)                                                                                                                                                                                                                                                   cACG‑TCACCATAGTGGTCGGT‑TGTGCTGTGAGGGTAGATAACGGC‑AACAATCGGTTTTTGCGGACGATGCTGGAAGTAAAGGTCAAGTGCGGCTTTTGCGGCAGGCGGCGTCACCAGCGGGTCGATAACAATAATGCCTTTCTCGCCCTCAACGAACGTAATGTTAGAGATATCCTGGCCGCGCACCTGATACATTTTATCGGTGACTTTGAACAGGCCAGAAATACCGTTGATTTGCGACTGACGCcac  <  2:54297/250‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                        CTGACGATAGTTTTCGTCGGTGCAATAATGCTCGGGTCGCCCGTTGCCAGCGTCACGCCAAGGCCATTACCCACATTGCCTTGCGCGTTGTGCGGCAGTAACAGACCGTAAGAGTAGAGCGCACGGCGGCTCATGATGTTACCCGCCAGCACGTTTTCGCTGATGGCTTCGTCCATAAAGCCTGCAGGGGCAATCACCTGAACTTTGCCGGATTTAACATCGGCTTCAGAGATAATGCCTTTCACG‑CCACCATAGTGGTCGGTGTG‑GCTGTGAGTGTAGATAACGGC‑AACAATCGGTTTTTGCGGACGATGCTGGAAGTAAAGGTCAAGTGCGGCTTTTGCGGCAGGCGGCGTCACCAGCGGGTCGATAACAATAATGCCTTTCTCGCCCTCAACGAACGTAATGTTAGAGATATCCTGGCCGCGCACCTGATACATTTTATCGGTGACTTTGAACAGGCCAGAAATACCGTTGATTTGCGACTGACGCCAC  >  NC_000913/4305789‑4306280

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑