Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,283,043	G→A	F11F (TTC→TTT)	yjcF ←	pentapeptide repeat‑containing protein YjcF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,283,043	0	G	A	100.0%	51.6 / NA	17	F11F (TTC→TTT)	yjcF	pentapeptide repeat‑containing protein YjcF
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (10/7);  total (10/7)

TATAAAATATTTATGTATTTTCCTGTCCGTTTCTTTCGTGATTTTTCCTGATGGTAAACTATCATAAATTCTTTTCATTTTCTGAATCAAGCCTATCACTTTTTCATGATCATCCATTTTGAAAAGAGAGAGGGCTTTATTGTCAATTAACGTGTTTTTATGATGTTTGCTGTAATTATCTGATTTTGTTTTCTTTGTATGTGATGGACTTGTGACAGAGTGGTTATCGTTAATCAGGCAAAGAGGGAAAAACATATTATTTAAACCATTATAGCGCATAAAATATCCTTATAATTAACAAGAAAGAAAAGGCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGT  >  NC_000913/4282797‑4283228                                                                                                                                                                                                                                                       |                                                                                                                                                                                          tattaaaTATTTATGTATTTTCCTGTCCGTTTCTTTCGTGATTTTTCCTGATGGTAAACTATCATAAATTCTTTTCATTTTCTGAATCAAGCCTATCACTTTTTCATGATCATCCATTTTGAAAAGAGAGAGGGCTTTATTGTCAATTAACGTGTTTTTATGATGTTTGCTGTAATTATCTGATTTTGTTTTCTTTGTATGTGATGGACTTGTGACAGAGTGGTTATCGTTAATCAGGCAAAGAGGaaaa                                                                                                                                                                                        <  2:429927/246‑1 (MQ=255) tataAAATATTTATGTATTTTCCTGTCCGTTTCTTTCGTGATTTTTCCTGATGGTAAACTATCATAAATTCTTTTCATTTTCTGAATCAAGCCTATCACTTTTTCATGATCATCCATTTTGAAAAGAGAGAGGGCTTTATTGTCAATTAACGTGTTTTTATGATGTTTGCTGTAATTATCTGATTTTGTTTTCTTTGTATGTGATGGACTTGTGACAGAGTGGTTATAGTTAATCAGGCAAAGAGGaaaa                                                                                                                                                                                        >  1:71779/1‑250 (MQ=255)     aaaTATTTATGTATTTTCCTGTCCGTTTCTTTCGTGATTTTTCCTGATGGTAAACTATCATAAATTCTTTTCATTTTCTGAATCAAGCCTATCACTTTTTCATGATCATCCATTTTGAAAAGAGAGAGGGCTTTATTGTCAATTAACGTGTTTTTATGATGTTTGCTGTAATTATCTGATTTTGTTTTCTTTGTATGTGATGGACTTGTGACAGCGTGGTTATCGTTAATCAGGCAAAGAGGAAAAAACat                                                                                                                                                                                   >  1:39313/1‑251 (MQ=255)               gttgtttCCTGTCCGTTTCTTTCGTGAGTTTTCCTGATGGTAAACTATCATAAATTCTTTTCATTTTCTGAATCAAGCCTATCACTTTTTCATGATCATCCATTTTGAAAAGAGAGAGGGCTTTATTGTCAATTAACGTGTTTTTATGATGTTGGCTGTAATTATCTGATTTTGGTTTAGTTGTATGTGATGGACTTGTGACAGAGTGGTTATCGTTAATCAGGCAAAGAGGAAAAAACATATTATTTaaa                                                                                                                                                                         <  2:169355/247‑1 (MQ=255)                 agtttCCTATCCGTTTCTTTCGTGATTTTTCCGGATGGGAAACTATCATAAAGTCTTTTCATTTTCTGAATCAAGCCTATCACTGTTTCATAATCATCCATTCTGAAAATAGATGGGGCTTTATTGTCAATTAACGTGTTTTTATGATGTTTGCTGTAATTATCTGATTTTGTTTTCTTTGTATGTGATATACTTGTGACAGATTGGTTATCGTTAATCAGGCAAAGAGGAAAAAACTTATTATTTAAAc                                                                                                                                                                        <  1:37875/248‑1 (MQ=255)                          tCCGTTTCTTTCGTGATTTTTCCTGATGGTAAACTATCATAAATTCTTTTCATTTTCTGAATCAAGCCTATCACTTTTTCATGATCATCCATTTTGAAAAGAGAGAGGGCTTTATTGTCAATTAACGTGTTTTTATGATGTTTGCTGTAATTATCTGATTTTGTTTTCTTTGTATGTGATGGACTTGTGACAGAGTGGTTATCGTTAATCAGGCAAAGAGGAAAAAACATATTATTTAAACCATTATAgcg                                                                                                                                                              >  1:506964/1‑251 (MQ=255)                              tttctttcGTGATTTTTCCTGATGGTAAACTATCATAAATTCTTTTCATTTTCTGAATCAAGCCTATCACTTTTTCATGATCATCCATTTTGAAAAGAGAGAGGGCTTTATTGTCAATTAACGTGTTTTTATGATGTTTGCTGTAATTATCTGATTTTGTTTTCTTTGTATGTGATGGACTTGTGACAGAGTGGTTATCGTTAATCAGGCAAAGAGGAAAAAACATATTATTTAAACCATTATAGCGCAt                                                                                                                                                           >  1:153191/1‑250 (MQ=255)                                    tcGTGATTTTTCCTGATGGTAAACTATCATAAATTCTTTTCATTTTCTGAATCAAGCCTATCACTTTTTCATGATCATCCATTTTGAAAAGAGAGAGGGCTTTATTGTCAATTAACGTGTTTTTATGATGTTTGCTGTAATTATCTGATTTTGTTTTCTTTGTATGTGATGGACTTGTGACAGAGTGGTTATCGTTAATCAGGCAAAGAGGAAAAAACATATTATTTAAACCATTATAGCGCATAAAata                                                                                                                                                     <  2:17477/250‑1 (MQ=255)                                    gctggATTTTTCCTTATGGTAAACTATAATAAATTCTTTTCAGTTTCTGAAGCAAGCCTATCACTTTTTCATGATCATTCATTTTGAAAAGAGAGAGGGCTTTATTGTCAATTAACGTGTTTGTATGATGTTTGCTGTAATTATCTGATTTTGTTTTCTTTGTATGTGATGGACTTGTGACAGAGTGGTTATCGTTAATCAGGCAAAGAGGAAAAAACATATTATTTAAACCATGATATCGCATAAAata                                                                                                                                                     <  2:212108/246‑1 (MQ=255)                                      gTGATTTTTCCTGATGGTAAACTATCATAAATTCTTTTCATTTTCTGAATCAAGCCTATCACTTTTTCATGATCATCCATTTTGAAAAGAGAGAGGGCTTTATTGTCAATTAACGTGTTTTTATGATGTTTGCTGTAATTATCTGATTTTGTTTTCTTTGTATGTGATGGACTTGTGACAGAGTGGTTATCGTTAATCAGGCAAAGAGGAAAAAACATATTATTTAAACCATTATAGCGCATAACATATcc                                                                                                                                                  >  1:434392/1‑251 (MQ=255)                                               ccttaTGGTAAACTATCATAAATTCTTTTCATTTTCTGAATCAAGCCTATCACTTTTTCATGATCATCCATTTTTAAAAGAGAGAGGTCTTTATTGTCAATTAACGTGTTTTTATGATGTTTGCTGTAATTATCTGATTTTGTTTTCTTTGAATGTGATGGACTTGTGACAGAGTGGTTATCGTTAATCAGGCAAAGATGAAAAAACATATTATTTAAACCATTATAGCGCATAAAATATCCTTATAATTa                                                                                                                                         <  2:34109/247‑1 (MQ=255)                                                     ggTAAACTATCATAAATTCTTTTCATTTTCTGAATCAAGCCTATCACTTTTTCATGATCATCCATTTTGAAAAGAGAGAGGGCTTTATTGTCAATTAACGTGTTTTTATGATGTTTGCTGTAATTATCTGATTTTGTTTTCTTTGTATGTGATGGACTTGTGACAGAGTGGTTATCGTTAATCAGGCAAAGAGGAAAAAACAtatt                                                                                                                                                                                >  1:363086/1‑206 (MQ=255)                                                     ggTAAACTATCATAAATTCTTTTCATTTTCTGAATCAAGCCTATCACTTTTTCATGATCATCCATTTTGAAAAGAGAGAGGGCTTTATTGTCAATTAACGTGTTTTTATGATGTTTGCTGTAATTATCTGATTTTGTTTTCTTTGTATGTGATGGACTTGTGACAGAGTGGTTATCGTTAATCAGGCAAAGAGGAAAAAACAtatt                                                                                                                                                                                <  2:363086/206‑1 (MQ=255)                                                      gTAAACTCTCATAAATTCTTTTCATTTTCTGAATCAAGCCTATCACTTTTTCATGATCATCCATTTTGAAAAGAGAGAGGGCTTTATTGTCAATTAACGTGTTTTTATGATGTTTGCTGTAATTATCTGATTTTGTTTTCTTTGTATGTGATGGACTTGTGACAGAGTGGTTATCGTTAATCAGGCAAAGTGGAAAAAACATATTATTTAAACCATTATATCGCATAAAATATCCTTATAATTAACaaga                                                                                                                                   >  2:46506/1‑250 (MQ=255)                                                                                                               catcCATTTTGAAAAGAGAGAGGGCTTTATTGTCAATTAACGTGTTTTTATGATGTTTGCTGTAATTATCTGATTTTGTTTTCTTTGTATGTGATGGACTTGTGACAGAGTGGTTATCGTTAATCAGGCAAAGAGGAAAAAACATATTATTTAAACCATTATAGCGCATAAAATATCCTTATAATTAACAAGAAAGAAAAGGCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCAttt                                                                         <  2:423737/251‑1 (MQ=255)                                                                                                                     ttttGAAAAGAGAGAGGGCTTTATTGTCAATTAACGTGTTTTTATGATGTTTGCTGTAATTATCTGATTTTGTTTTCTTTGTATGTGATGGACTTGTGACAGAGTGGTTATCGTTAATCAGGCAAAGAGGAAAAAACATATTATTTAAACCATTATAGCGCATAAAATATCCTTATAATTAACAAGAAAGAAAAGGCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAAt                                                                   >  1:176125/1‑251 (MQ=255)                                                                                                                                               tCAATTAACGTGTTTTTATGATGTTTGCTGTAATTATCTGATTTTGTTTTCTTTGTATGTGATGGACTTGTGACAGAGTGGTTATCGTTAATCAGGCAAAGAGGAAAAAACATATTATTTAAACCATTATAGCGCATAAAATATCCTTATAATTAACAAGAAAGAAAAGGCATATTCTATGCATTATCATTTTCTAAGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATggg                                          >  2:381435/1‑250 (MQ=255)                                                                                                                                                                   atgTTTGCTGCAATTATCTGATTTTGTTTTCTTTGTATGTGATGGACTTGTGACAGAATGGTTATAGTTATTCAGGCACAGAGGAAAAAACATATTATTTAAACCCTTATAGCGCATACAATATCCTTATAATTAACAAGAAAAAAAAGGCATATTCTCTGAATTATCATTTTCTACGCCTGTCACAACTCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTTTGGGAAAAGGTGATGAATTTggg                       >  2:457059/1‑249 (MQ=255)                                                                                                                                                                                      gATTTTGTTTTCTTTGTATGTGATGGACTTGTGCCAGAGTGGTTATCGTTAATCAGGCAAAGAGGAAAAGACATATTATTTAAACCATTATAGCGCATACAATATCCTAATAATTAACAAGAAAGAAAAGGCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGt  >  1:285721/1‑251 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                          TATAAAATATTTATGTATTTTCCTGTCCGTTTCTTTCGTGATTTTTCCTGATGGTAAACTATCATAAATTCTTTTCATTTTCTGAATCAAGCCTATCACTTTTTCATGATCATCCATTTTGAAAAGAGAGAGGGCTTTATTGTCAATTAACGTGTTTTTATGATGTTTGCTGTAATTATCTGATTTTGTTTTCTTTGTATGTGATGGACTTGTGACAGAGTGGTTATCGTTAATCAGGCAAAGAGGGAAAAACATATTATTTAAACCATTATAGCGCATAAAATATCCTTATAATTAACAAGAAAGAAAAGGCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGT  >  NC_000913/4282797‑4283228

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑