Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,424	A→T	A208A (GCA→GCT)	thrB →	homoserine kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,424	0	A	T	100.0%	161.1 / NA	53	A208A (GCA→GCT)	thrB	homoserine kinase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (21/32);  total (21/32)

TGGGCGAGCTGGAAGGCCGTATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTCGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCAGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCAGGCTTCCGGCAGGCGCGGCAGGCGGTCGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGAAACCGCCCAGCGCGTTGCCGACTGGTTGGGTAAGAACTACCT  >  NC_000913/3180‑3663                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                tGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCTGGCttc                                                                                                                                                                                                                                           >  1:304675/1‑251 (MQ=255)   ggCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTGACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCTGGCttcat                                                                                                                                                                                                                                         <  1:259270/251‑1 (MQ=255)     cGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCTGGCttcattc                                                                                                                                                                                                                                       <  1:46101/251‑1 (MQ=255)                         ccGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCTGGCTTCATTCACGCCTGCTATTCCCg                                                                                                                                                                                                                       <  1:243706/247‑1 (MQ=255)                            gcagcaTTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCt                                                                                                                                                                                                                <  2:411007/251‑1 (MQ=255)                                cattcattTCGACAACGTGGCACCGGGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGGGCCAGGGTTTGATGAGGTGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGGCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGc                                                                                                                                                                                                            <  2:366256/251‑1 (MQ=255)                                     attACGACAACGTGGAACCGGGTTTTCTTGGTGGTATGAAGTTGATGATAGAAGAAAACGCCATCATAAGCCAGCAAGTGCCAGGGTTTGATGAGTGGCTGTGGGTGATGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCAAGGGCTATTTTACCGGCGACGCAACGTCACCGGGTTTGCAATGCGAACGGGCGCAATCTGGCTGGCTTCCTTCACGCCTACTATTCCCCTCAGCCTGAGCTTGcc                                                                                                                                                                                                       >  2:251027/1‑251 (MQ=255)                                       tACGACAACGTGGCACCGTGTTTTCTGGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCcg                                                                                                                                                                                                      <  2:81131/250‑1 (MQ=255)                                           aCAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAg                                                                                                                                                                                                 <  1:136292/251‑1 (MQ=255)                                             aaCGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCTGGCTTCATTCCCGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCt                                                                                                                                                                                               >  2:387818/1‑251 (MQ=255)                                             aaCGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGAGGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCGAGTGCGATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCt                                                                                                                                                                                               <  1:207515/251‑1 (MQ=255)                                                  acacgacgcttttccgaatggtAGGCAGTTGAGGATCGAAAAAAACGGCAACATCAGCCAGCAAGTGCCAGGGTTTGATGAGGGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGGCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTACCGCCGCCAGGATTGCATTGCGCTCGGGCGACATCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCtgatg                                                                                                                                                                                           <  2:467469/232‑1 (MQ=255)                                                     cccGTGTTTTCTTGGTGGTAGGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGTGGCTGTGTGTGCGGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAg                                                                                                                                                                                       <  2:195949/250‑1 (MQ=255)                                                       cGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGAt                                                                                                                                                                                     <  1:216472/251‑1 (MQ=255)                                                              tcttggtggtACGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCATGGTTTGATGAGTGGCTGTGGGTGCTGGCGGATCCGGGGATTAAAGTCGCGACTGCAGAAGTTAGGGCTATTTTACCGGCGCAGTATCTCTGCCAGGATTTCATTTCGCACGGGCGACATCTGGCTGGCGTCATTCGCGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATTTTATc                                                                                                                                                                               <  1:64840/246‑1 (MQ=255)                                                                 tggtggtATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGAGGAGTGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCg                                                                                                                                                                              <  1:121437/247‑1 (MQ=255)                                                                      gtATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGAATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCCGGATTGCATTGCGCACGGGCGACATCTGGCTGGCTTCCTTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCtgatg                                                                                                                                                                                           >  1:467469/1‑230 (MQ=255)                                                                             gTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGtt                                                                                                                                                               >  1:85273/1‑250 (MQ=255)                                                                             gTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGGCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTg                                                                                                                                                               <  2:228234/251‑1 (MQ=255)                                                                                     tCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGtt                                                                                                                                                        >  1:477833/1‑250 (MQ=255)                                                                                             aaCGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGAAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAGAGATGTTATCGCTGAACCCTACCGTGCACGGTTACTGcctgg                                                                                                                                               >  1:389124/1‑248 (MQ=255)                                                                                                  catcatCAGCCAGCAAGTGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCGGAACCCTACCGTGAACGGTTACCGCCTGGCTTcc                                                                                                                                          >  2:284576/1‑251 (MQ=255)                                                                                                     catcaGCCAGCAAGTGCCAGGGTTTGATGAGTGGCTGTGGGAGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCCTTGCGCACGGGCGACATCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGCAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCcggc                                                                                                                                       >  1:391140/1‑251 (MQ=255)                                                                                                      atcaGCCAGCAAGTGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGCCGGCAGAAGCCAGGGCTATTTTACCGGCGCCGTATCGCCGCCCGGCTTGCATTGCGCACGGGCGACATCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCCGCCTGTGCTTGCCGCGTGTCTGCTGAAAGCTGTTATCGCTGAACCCTACCGTGGACGGTTACTGCCTGGCTTCcggc                                                                                                                                       >  2:170277/1‑250 (MQ=255)                                                                                                         agccagcAAGTGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCcggcaggc                                                                                                                                   <  1:358672/251‑1 (MQ=255)                                                                                                                  gTGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCTGGCttc                                                                                                                                                                                                                                           >  2:224246/1‑138 (MQ=255)                                                                                                                  gTGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCTGGCttc                                                                                                                                                                                                                                           <  1:224246/138‑1 (MQ=255)                                                                                                                       aGGGTTTGATGATTGGCTGTGGGTGCGAGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGttg                                                                                                                     <  2:347416/251‑3 (MQ=255)                                                                                                                                 gaGTGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCcggcagg                                                                                                                                    <  1:476971/226‑1 (MQ=255)                                                                                                                                 gaGTGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCcggcagg                                                                                                                                    >  2:476971/1‑226 (MQ=255)                                                                                                                                       cTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCcggcag                                                                                                                                     >  1:69265/1‑219 (MQ=255)                                                                                                                                       cTGTGGGTGCTGGCGTATCCGGGGATTAAAGGCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCcggcag                                                                                                                                     <  2:69265/219‑1 (MQ=255)                                                                                                                                               gCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGAAATCGGCGCGGTagcgagcg                                                                                             <  1:386380/251‑1 (MQ=255)                                                                                                                                               gCTGGCGTATCCGGGGATTAAAGTCCCGACGGCAGAAGCCAGGGCTATTTTGCCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCTGGCTTTATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAGCCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGAAATCGGCGCGGTagcgagcg                                                                                             <  1:174705/251‑1 (MQ=255)                                                                                                                                                     gTATCCGGGGATTAAAGTCTCGACGGCAGAAGACAGGGCTATTTTACCGGCCCAATATCGACGCCAGGATTGCATTGCGCACGGGCGACATCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGCTGTTCTCCCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGCTTCGGCAATACGCGCGGTAGCGACCGGGAtct                                                                                       >  1:307419/1‑251 (MQ=255)                                                                                                                                                        gccGGGGATTAAAGCCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACTCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGAAATCGGCGCGGTAGCGAGCGGTATctccg                                                                                    <  2:391140/250‑1 (MQ=255)                                                                                                                                                              gattaaatgcccGACTGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGAAATCGGCGCGGTAGCGAGCGGTATctccggctccg                                                                              <  2:1796/240‑1 (MQ=255)                                                                                                                                                              gATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGAAATCGGCGCGGTAGCGAGCGGTATctccggctccg                                                                              <  1:91435/251‑1 (MQ=255)                                                                                                                                                                               cAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTTCGGAAATCGGCGGGGTTGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGcc                                                             >  1:225365/1‑250 (MQ=255)                                                                                                                                                                                       aGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGGTGCGGGAATCGGCGCGGTAGCGAGGGGTATCACAGGCTCCGGGCCGGACTTGTTCGCTCtgtgtgg                                                     >  1:94451/1‑250 (MQ=255)                                                                                                                                                                                           cTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTCACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGATGCGGAACTATGGGCGGGAGGGGGCGGTATCTCCGGCGCCGGACCGACCTTGGTTGGTCTGGGTGACac                                                  >  1:36617/1‑249 (MQ=255)                                                                                                                                                                                             aTTTTACCGGCGCAGTTTCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCTGGCTACATTCACGCCTGCTATGCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGAAATCGGCGCGGTATCGAGCGGGATCTCCGGCTCCGGCCCGACCTTGTTCGCTCGGTGTGACAAg                                                 <  2:299963/249‑1 (MQ=255)                                                                                                                                                                                               tttcccGGCGCAGTACCGCCGCCAGGATTGCATGGCGCACGGGCGACATCTGGCTGGCTTCATTCCCGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGAGGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGGTCGCTCTGTGTGACAAGCCg                                              <  2:310392/246‑1 (MQ=255)                                                                                                                                                                                                 tcccGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGTGCGGCATCGGGCTGGCTTCCTTCACGCCTTCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGa                                            <  2:147606/248‑1 (MQ=255)                                                                                                                                                                                                 tACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGAAATCGGCGCGGTAGCGAGCGGGATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGat                                           >  1:300369/1‑250 (MQ=255)                                                                                                                                                                                                               cgccgcCAGGATTGCATTGCGCACGGGCGACATCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACg                                                                                                                                                           >  2:142412/1‑125 (MQ=255)                                                                                                                                                                                                                    ccAGGATTGCATTGCGCACGGGCGACATCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGATACCGCCCAGCGCGTTGCCg                        <  1:192633/251‑1 (MQ=255)                                                                                                                                                                                                                            gcattgcGCACGGGCGACATCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGATACCGCCCAGCGCGTTGCCGACTGGtt                 <  1:338302/250‑1 (MQ=255)                                                                                                                                                                                                                            gcattgcGCACGGGCGACATCTGGCTGGCTTCATTCACGCCTGCTATTCCCGGCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGATACCGCCCAGCGCGTTGCCGACTGGtt                 <  2:300369/250‑1 (MQ=255)                                                                                                                                                                                                                             cattgcGCACGTGCGACATCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGAGAAGCTGATGAAAGATGTTATCGCCGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGAAATCGGCGCGGTAGCGAGCTGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGATACCGCCCAGCGCGTTGCCGACTGGTTgg               <  1:21397/251‑1 (MQ=255)                                                                                                                                                                                                                                    cACGGGCGACATCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGGTACCGCCCAGCGCGTTGCCGACTGGTTGGGGAAGa         >  1:287285/1‑250 (MQ=255)                                                                                                                                                                                                                                         gCGACATCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTACCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGAAATCGGCGCGGTAACGAGCGGTCTCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGGCCAGCCGGATACCGCCCAGCGCGTTGCCGACCGGTTGGGTAAGAACtccc   >  1:164830/1‑248 (MQ=255)                                                                                                                                                                                                                                          cGACATCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGAAATCGGCGCGGTAGCGATCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGATACCGCCCAGCGCGTTGCCGACTGGTTGGGTAAGAACTACCt  <  1:213645/251‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                TGGGCGAGCTGGAAGGCCGTATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTCGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCAGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCAGGCTTCCGGCAGGCGCGGCAGGCGGTCGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGAAACCGCCCAGCGCGTTGCCGACTGGTTGGGTAAGAACTACCT  >  NC_000913/3180‑3663

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑