Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,193,996	T→G	D70A (GAT→GCT)	thiE ←	thiamine phosphate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,193,996	0	T	G	97.7%	135.2 / NA	45	D70A (GAT→GCT)	thiE	thiamine phosphate synthase
Reads supporting (aligned to +/- strand):  ref base T (1/0);  major base G (21/22);  minor base A (1/0);  total (23/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

GTGCCAGCTGTTCCAGCCCCTGCGGTGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAATCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCG‑CCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCAGGCTGATACATCACGCTTCCTCCTTACGCAGGTAGATTTCTC  >  NC_000913/4193754‑4194235                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                 gTGCCAGCTGTTCCAGCCCCGGCGGTGCAGAAGGCAGCGGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATa                                                                                                                                                                                                                                          <  1:316407/251‑1 (MQ=255)       gCTGTTCCAGCCCCTGCGGTGCAGAAGGCATATGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATACGAGGGGCGTGCTGCCAGAGCGACGTCGCTTTCCCTATCGTCATGTGTCGCAACGCCCAGCCGCAGGCCTGACGCGCGGGTGGCATTGAGTTCGCTGGCTTGCAACGTTTCCTGCCACAAATGGACGACATACGCAGGATGCTTGATAGACAGACGCCCCTAGTCGTTGATaaccac                                                                                                                                                                                                                                     >  2:491394/1‑246 (MQ=255)        cTGTTCCAGCCCCTGCGGTGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAACTCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGCAAGCGTTGATAAACAAt                                                                                                                                                                                                                                    >  2:266326/1‑250 (MQ=255)                gCCCCTGCGGTGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTAATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTta                                                                                                                                                                                                                           <  1:383906/251‑1 (MQ=255)                 ccccTGTGGTGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGGGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATAGCGTAATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTtat                                                                                                                                                                                                                          <  2:105919/251‑1 (MQ=255)                  cccTGCGGTGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTtat                                                                                                                                                                                                                          >  1:243527/1‑250 (MQ=255)                      gggttGCAGAAGGCATCTGTTTGGTTTGCGGCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGAGCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAgc                                                                                                                                                                                                                       <  1:441528/245‑1 (MQ=255)                                   cctCTGCTTGGATTGCGTCGGGAACATATGTCCCAGCGCGCTTTAAGATGTGCGTGCTGCCAGCGCGCCGTCGATTTCCATCTTGTCATGTGTCGTAACGCGCAGCCCCAGGCCTGCCGCGCGGATGGCATTGAGCTCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACCCCATACCCCTGCAGCCTGATCCCCAGCCGACAGTAAACGTTGAGAAACAATCGCGCGTTATAGAGGCAGCCCCGCGCa                                                                                                                                                                                                         >  2:389270/3‑250 (MQ=255)                                        gtttggtgggggTCGGAAACACATGCCCCAGCGCGATAAAAGGGGGGGGTGCGGCACGCGCGACGTCGATTTCCATATCGCCATGGGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTGCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTgcc                                                                                                                                                                                                   <  2:458022/240‑1 (MQ=255)                                             gtttgCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTTTAGCGGCGGCCCAACGCAAATGCCG‑CCAc                                                                                                                                                                                             >  2:310304/1‑251 (MQ=255)                                                     cGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCG‑CCACGACATCgg                                                                                                                                                                                     >  1:318170/1‑251 (MQ=255)                                                        gAACCCATGTCCTAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCAAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCGGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATAGCGCGTTATAGCGGCGGCCCAGTGCAATTGCCG‑CCACGACATCGGCtt                                                                                                                                                                                  <  1:457186/251‑1 (MQ=255)                                                        gAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCG‑CCACGCCATCGGCtt                                                                                                                                                                                  >  1:498735/1‑251 (MQ=255)                                                             caTGTCCCAGCGCGATATAAGAGGGGCGTGCTACCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGCAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAACTCTTCCTGCCCCAAATGGACGCCATACGCCTGCTGCTTGATCGCAAGCCGCCCGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCG‑CCCCCACATCGGCTTCCaac                                                                                                                                                                             >  2:244716/1‑249 (MQ=255)                                                                gTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGAGGCTTGCAAATCTTCCTACCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGACCA‑GACATAGCCTTCCACctct                                                                                                                                                                          >  1:64064/1‑251 (MQ=255)                                                                     ggcgcTATATAAGGGGGGGGTGCGCCCAGCGCGGCGTCGATTCCCATTGCGACAGGGGTCAAACCGCCCCGCCGCAGGCCGGCCGCGCGGAGGGCATGGAGAGCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGAGGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCG‑CCACGACATCGGCTTCCACCTCTTCATc                                                                                                                                                                     <  2:195889/250‑1 (MQ=255)                                                                           atatAAGGGGGGCGTGCTGCCGGCTCGACGTCGATTGCCATATCGTCATTTGTCTAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGAGCGGTGGCTTGCAAAACTGCCTGCCCCAAATGGACGCCATACGCCGGATGTTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCG‑CCACGACATCGGCTTCCACCTCTTCATCGCGCCg                                                                                                                                                               <  1:36894/251‑1 (MQ=255)                                                                              aaaggggggCGTGCTGCCAGCGGGAAGTCGTTTTCCATAGCGACTGGTGGAGACACGCCCAGCCGCGGGCCGGCCGCGCGGAGGGCATTGAGATCGGTGGCTTGCAAATCCTCCTGCCCCAAAGGGCCGCCATCCGCCTGAGGCTTGACCGCCAGCCGCCAGTAAGCGTTGATAACCAAGCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCG‑CCACGACATCGGTTTCCACCTCTTCATCGCGCCGATc                                                                                                                                                            <  2:227659/246‑1 (MQ=255)                                                                                 gagGGGCGGGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCG‑CCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCttt                                                                                                                                                         <  1:485388/251‑1 (MQ=255)                                                                                                     gaggtggtttaccATATCTTCATGGGCCAAAACGCCCGGCCGCAGCCCTCCCGCGCTGATGGCATTGAGATCGGGGGCTTGAAAATTTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCG‑CCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAgtac                                                                                                                                     <  2:392379/240‑1 (MQ=255)                                                                                                      aCGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGccc                                                                                                                                                                                                               >  2:318917/1‑177 (MQ=255)                                                                                                      aCGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCGGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGccc                                                                                                                                                                                                               <  1:318917/177‑1 (MQ=255)                                                                                                                   atgtctgtttggacaaaccGCCCCGCCGCGGGCCGGCCCGGCGGATGGCTTTGAGGTCGGGGGCTGAAAAATTTTCCGTCCACAAATGGACGCCATCCGCCGGATGTGTGTCCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATGGCCG‑CCACGACACCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATcc                                                                                                                       <  2:270439/233‑1 (MQ=255)                                                                                                                          aTGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCG‑CCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGAc                                                                                                                >  1:176122/1‑251 (MQ=255)                                                                                                                            gggccGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGAAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGACCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCG‑CCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACg                                                                                                               <  1:9833/246‑1 (MQ=255)                                                                                                                                 gAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCG‑CCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGAt                                                                                                         >  1:496402/1‑251 (MQ=255)                                                                                                                                          aGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCG‑CCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCCTCCAACAGACGTTCGATCCACTGTAc                                                                                                >  1:209789/1‑251 (MQ=255)                                                                                                                                             cGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAACTCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGATATAGCGGCGGCCCAGCGAAATTTCCG‑CCACGACCTCGGCTTACACCCCTTCATCGAGCCGGTCTTTCATGCGGATCTGGAGAGTACGTACGCCTGCACCCAACAGACGTTTGATCCACttggagct                                                                                             >  2:98257/1‑244 (MQ=255)                                                                                                                                                                    aTTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCG‑CCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTg                                                                      >  1:290387/1‑251 (MQ=255)                                                                                                                                                                       gatatctgTGGTTTGCAAATCTTCCTCCCCAAAATGGCCGCCATCCGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCTTTGATAAACAATCGCGCGTAATAGCGGCGGCCCAGCGCAATTGCCG‑CCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCATCAGACGTTCGATCCACTATACGCTGTCCACCACCGGGTACAGTCCTGaa                                                                    <  1:292838/243‑1 (MQ=255)                                                                                                                                                                                ggCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCG‑CCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCCCCGGGTACAGTCCTGAACGAAAAGGt                                                           >  1:199610/1‑250 (MQ=255)                                                                                                                                                                                 gCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGACCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCG‑CCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTAc                                                         <  1:310304/251‑1 (MQ=255)                                                                                                                                                                                  attgaaaaTCTTCCGTCCCAAAATGGACTCCATACGCCGTATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAATCAATCTCGCTTTATAGCGGCGGCCCAGCGCAATTGCCG‑CCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAACAGGTACa                                                        <  1:315837/246‑1 (MQ=255)                                                                                                                                                                                        aaTCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCG‑CCACGACATCGGCTTCCACCTCTTCATCGCGACGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACaggagg                                                   >  1:336157/1‑250 (MQ=255)                                                                                                                                                                                        aaTCTCCCTGCCCCAAAGTGACGCCATACGCCTGATGTTTGAGCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCTTTATAGCGGCGGCCCAGCGCAATTGCCG‑CCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAggagga                                                  <  1:32597/251‑1 (MQ=255)                                                                                                                                                                                                cgCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCG‑CCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCAgg                                          <  1:123081/250‑1 (MQ=255)                                                                                                                                                                                                      aaaTGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCG‑CCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCAGGCTGAt                                     >  2:66102/1‑250 (MQ=255)                                                                                                                                                                                                       aaTGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCG‑CCACGCCCTCGGCTTCCACCTCTTCATCGCGCCGATCTTTTGTGCGTAGCTGGGGAGTACGTACGCCTGCATCCACCAGACGTTCGATCCACTTTACGATGTCCACCACCGGGTACCGTCCTGAACGCAAAGGAAAAGGCAGAAATTCATGCTGATAc                                   >  2:417526/1‑251 (MQ=255)                                                                                                                                                                                                          ggACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCG‑CAACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTACTGAACGAAAAGGTACAGGAGGAAAATCAGGCTGATAAATc                                >  1:294061/1‑251 (MQ=255)                                                                                                                                                                                                                    cgctcgattctttcccccctgccgcgAGTAAGTGTTCATAAACAAGCGCGCGTTATAGCGGCGGCCCAGCGCTATTGCCG‑CAACGACATCGGCTTCCCCCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCAGGCTGATACATCACGCTtcctc                      <  2:126048/231‑1 (MQ=255)                                                                                                                                                                                                                    cgccggaggtgttaaccccAGCCCCCAGTAAGCGTGGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCG‑CCTCGACAGCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCAGGCTGATACATCACGCTtcctc                      <  2:358964/234‑1 (MQ=255)                                                                                                                                                                                                                        ggaggatggATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCG‑CCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCAGGCTGATACATCACGCTTCCTCCTTa                  <  1:68482/243‑1 (MQ=255)                                                                                                                                                                                                                           tGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCG‑CCACGACATCGGCTTCCACCTCTTCATCGCGCCGCTCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGAACAGGAGGAAAATCAGGCTGATACATCACGCTTCCTCCTTACGc               >  2:473667/1‑251 (MQ=255)                                                                                                                                                                                                                                 aTCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCG‑CCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCAGGCTGATACATCACGCTTCCTCCTTACGCAGGt           >  1:58199/1‑249 (MQ=255)                                                                                                                                                                                                                                        gccgccAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCG‑CCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTAGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGAACAGGAGGAAAATCAGGCTGATACATCACGCTTCCTCCTTACGCAGGTAGATTtctc  >  2:479500/1‑251 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                 GTGCCAGCTGTTCCAGCCCCTGCGGTGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAATCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCG‑CCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCAGGCTGATACATCACGCTTCCTCCTTACGCAGGTAGATTTCTC  >  NC_000913/4193754‑4194235

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑