Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,133,252	C→T	P213S (CCG→TCG)	metF →	5,10‑methylenetetrahydrofolate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,133,252	0	C	T	100.0%	123.1 / NA	38	P213S (CCG→TCG)	metF	5,10‑methylenetetrahydrofolate reductase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (9/29);  total (9/29)

CTTCTGACCTGGTGACGCTGTTAAAAGAAGTGGCAGATTTCGATATCTCCGTGGCGGCGTATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTCCGGGAATTTTGCCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGTGAAGGAGTGAAAGATTTCCACTTCTATACGCTTAACCGTGCTGAAATGAGTTACGCGATTTGCCATACGCTGGGGGTTCGACCT  >  NC_000913/4133010‑4133497                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                      gttcgttccgggTGACGCTGTTAAAAGAAGTGGCAGATTTCGATAGCTCCGTGGCGGCGTATCCGGAAGTGCACCCGGAAGCAAAAAGCGCACAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGACGGAGTCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTCCGGGAATTTTGTCGGTATcg                                                                                                                                                                                                                                               <  2:416628/241‑2 (MQ=255)   tCTGACCTGGTGACGCTGTTAAAAGAAGTGGCAGATTTCGATATCTCCGTGGCGGCGTATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGTTGTGGGAATTATTCCCGGGATTTTGTCGGTGTCTa                                                                                                                                                                                                                                              >  1:78238/1‑250 (MQ=255)                cGCTGTTAAAAGAAGTGGCAGATTTCGATATCTCCGTGGCGGCGTATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATGGATGTGGAAATTATTCCGGGAATTTTGTCGGTATCTAACTTTAAACAGGCg                                                                                                                                                                                                                                <  1:323728/251‑1 (MQ=255)                          agaagTGGCAGATTTCGATATCTCCGTGGCGGCGTATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTCCGGGAATTTTGTCGGTATCTAACTTTAAACAGGCGAAGAAATTTg                                                                                                                                                                                                                      <  1:3220/251‑1 (MQ=255)                                ggCAGATTTCGATATCTCCGTGGCGGCGTATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTCCGGGAATTTTGTCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGata                                                                                                                                                                                                                <  1:422730/251‑1 (MQ=255)                                 gCAGATTTCGATATCGCCGTGGCGGCGTATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTCCGGGAATTTTGTCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGatat                                                                                                                                                                                                               <  2:346968/251‑1 (MQ=255)                                   aGATTTCGATATCTCCGTGGCGGCGTATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGTTGTGGGAATTTTTTCGGGGATTTTTTCGGTGTTTTACTTTTAACAGGCgaaagaatttttccgtatg                                                                                                                                                                                                              >  1:255128/1‑234 (MQ=255)                                         cGATATCTCCGTGGCGGCGTATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTCCGGGAATTTTGTCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAAc                                                                                                                                                                                                        <  1:224344/250‑1 (MQ=255)                                         cGATATCTCCGTGGCGGCGTATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTCCGGGAATTTTGTCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACg                                                                                                                                                                                                       <  1:126228/251‑1 (MQ=255)                                                             aTCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTCCGGGAATTTTGTCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTGGATg                                                                                                                                                                                   <  2:311190/251‑1 (MQ=255)                                                             aTCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTCCGGGAATTTTGTCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTGGATg                                                                                                                                                                                   <  1:100988/251‑1 (MQ=255)                                                                          cccGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTCCGGGAATTTTGTCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCg                                                                                                                                                                      <  1:379257/251‑1 (MQ=255)                                                                                       aGCGCTCAGGCGGATTTGCTCAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCGTCGATGTCGAAAGCTTCCTGCGTTTTCGTGACCGCGGTGTATCGGCGGGCATTGATGTGGAAATTATTCCGGGAATTTTGTCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGgatga                                                                                                                                                         <  2:175307/251‑1 (MQ=255)                                                                                             cAGGCGGATTTGCTGAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTCCGGGAATTTTGTCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGc                                                                                                                                                   <  1:245470/251‑1 (MQ=255)                                                                                              aGGCGGATTTGCTTAAACTGAAACGAAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGGCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGTGGGCATTGATGTGGAAAGTATTCCGGGAATTTTGTCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTTGATGATGATGcc                                                                                                                                                  <  2:341953/251‑1 (MQ=255)                                                                                                          ttAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGGAATTTTTTCGGGGATTTTTTCGGTATCTAACTTTTAACAGGCGAAGAAAATTTCCGATATGACCAACGTGCGTATTTCGGCGGGGGTGGCGCAAATGTTCGGAGGGCTGGGTGGTGATGCCGGAAcccccca                                                                                                                                       >  1:89512/1‑246 (MQ=255)                                                                                                            aaTCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTCCGGGAATTTTGTCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACt                                                                                                                                    <  2:89512/251‑1 (MQ=255)                                                                                                                 gAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGTAAGCTACCTTCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGGAATTATTCCGGGAATTTTGTCGGTATCTAACGTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGt                                                                                                                                 <  1:465194/249‑1 (MQ=255)                                                                                                                 gAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCTTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATTTGGAAATTATTCCGGGAATTTTGTCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTg                                                                                                                                   <  1:55720/247‑1 (MQ=255)                                                                                                                            tGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTTGTGTGGGAATTATTTCGGGGATTTTTTTGGGATCTAACTTTAAAAAAGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTTCGGCGGGGGTGGCGGAAATGTTCGCCGGGCTGGGTGATGATGCCGAAAACCGCCAACTCGGTGGcgcggctatt                                                                                                                    >  1:229124/1‑245 (MQ=255)                                                                                                                                      aGCCAACCGCGCGATTACGCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGAAGTGGAAATTATTCCGGGAATTTTGTCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTGGATGGCGCAAATg                                                                                                                                                                          <  2:23000/187‑1 (MQ=255)                                                                                                                                        ccAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTCCGGGAATTTTGTCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATg                                                                                                        <  1:410195/251‑1 (MQ=255)                                                                                                                                         cAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTCCGGGAATTTTGTCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATgg                                                                                                       <  2:78238/251‑1 (MQ=255)                                                                                                                                                      aCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTCCGGGAATTTTGTCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAg                                                                                          <  1:347904/251‑1 (MQ=255)                                                                                                                                                        tCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTCCGGGAATTTTGTCGGTATCTAACTTTAAACATGCGAAGAAATTTGCCg                                                                                                                                                                                                                   <  2:168294/128‑1 (MQ=255)                                                                                                                                                         cAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCACTGATGTGGAAAATAATCCGGGGATTTTGTCGGGATCTAACTTTAAACAGGAGAAGAAATTTGTCGATATGAACAACGTGGGTATTCCCGCGGGGGGGGCGCAAATGTTTGCCGGGCTGGGTGATGATGCCGAAAACCGCAAACAGGGTGGCGCGAATATTGTCATGGAGATGGTGGAGAATTTAAGCCg                                                                                       >  1:339231/1‑251 (MQ=255)                                                                                                                                                                   tcGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTCCGGGAATTTTGTCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGTGAAGGa                                                                                <  1:46992/248‑1 (MQ=255)                                                                                                                                                                                       cGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTCCGGGAATTTTGTCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGTGAAGGAGTGAAAGATTTCCACTTCTATAc                                                         <  1:280529/251‑1 (MQ=255)                                                                                                                                                                                           ttCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTCCGGGAATTTTGTCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGTCGTGAAGGAGTGAAAGATTTCCACTTCTATACGCt                                                      <  1:165662/250‑1 (MQ=255)                                                                                                                                                                                             cGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTCCGGGAATTTTGTCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGTGAAGGAGTGAAAGATTTCCACTTCTATACGCTTaa                                                   <  1:396078/251‑1 (MQ=255)                                                                                                                                                                                                           aTCGGCGGGCATTGATGTGGAAATTATTCCGGGAATTTTGTCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGTGAAGGAGTGAAAGATTTCCACTTCTATACGCTTAACCGTGCTGAAATGa                                     <  1:60552/251‑1 (MQ=255)                                                                                                                                                                                                                     aTTGATGTGGAAATTATTCCGGGAATTTTGTCGGTATCTAACTTTAAGCAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTGGa                                                                                                                                                                                     >  1:388870/1‑97 (MQ=255)                                                                                                                                                                                                                     aTTGATGTGGAAATTATTCCGGGAATTTTGTCGGTATCTAACTTTAAGCAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTGGa                                                                                                                                                                                     <  2:388870/97‑1 (MQ=255)                                                                                                                                                                                                                           gtgGAAATTATTCCGGGAATTTTGTCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGTGAAGGAGTGAAAGATTTCCACTTCTATACGCTTAACCGTGCTGAAATGAg                                    <  1:92176/236‑1 (MQ=255)                                                                                                                                                                                                                           gtgGAAATTATTCCGGGAATTTTGTCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGTGAAGGAGTGAAAGATTTCCACTTCTATACGCTTAACCGTGCTGAAATGAg                                    >  2:92176/1‑236 (MQ=255)                                                                                                                                                                                                                                  ttattCCGGGAATTTTGTCGGTATCTAACTTTAAACAGGCGAAGAACTTTGCCGATATGACCAACGTGCGTATTACGGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGCATATTGCCATGGATATGGTGAAGATTTTAAGCCGTGAAGGAGTGAAAGATTTCCACTTCTATACGCTTAACCGTGCTGAAATGAGTTACGCGATTTGCCATACGCTg              >  1:205314/1‑251 (MQ=255)                                                                                                                                                                                                                                      tCCGGGAATTTTGTCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGTGAAGGAGTGAAAGATTTCCACTTCTATAAGCTTAACAGTGCTGAAATGAGTTACGCGATATGCCATACGCTggggg          >  2:83134/1‑251 (MQ=255)                                                                                                                                                                                                                                               tttGTCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGTGAAGGAGTGAAAGATTTCCACTTCTATACGCTTAACCGTGCTGAAATGAGTTACGCGATTTGCCATACGCTGGGGGTTCGACCt  <  1:394986/250‑1 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                      CTTCTGACCTGGTGACGCTGTTAAAAGAAGTGGCAGATTTCGATATCTCCGTGGCGGCGTATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTCCGGGAATTTTGCCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGTGAAGGAGTGAAAGATTTCCACTTCTATACGCTTAACCGTGCTGAAATGAGTTACGCGATTTGCCATACGCTGGGGGTTCGACCT  >  NC_000913/4133010‑4133497

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑