Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,931,779	T→C	F155S (TTT→TCT)	kup →	K(+):H(+) symporter Kup

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,931,779	0	T	C	100.0%	136.4 / NA	44	F155S (TTT→TCT)	kup	K(+):H(+) symporter Kup
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (19/25);  total (19/25)

ATGCGGGCAGATAACGCCGGTGAGGGGGGGATCCTGACGTTGATGTCGCTTGCCGGGCGTAATACGTCGGCGCGAACCACATCAATGCTGGTG‑ATTATGGGGCTAATCGGCGGCAGCTTTTTCTATGGTGAAGTCGTCATAACACCCGCTATTTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATACCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATTTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAG‑TGGTGTTGTCGATTACGGGGGTCGAGGCGCTGTATGCTGATATGGGG  >  NC_000913/3931532‑3932023                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                      aTGCGGGCAGATAACGCCGGTGAGGGGGGGATCCTGACGTTGATGTCGCTTGCCGGGCGTAATACGTCGGCGCGAACCACATCAATGCTGGTG‑ATTATGGGGCTAATCGGCGGCAGCTTTTTCTATGGTTAAGTCGTCATAACTCCCGCTATTTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATACCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTAttatctat                                                                                                                                                                                                                                                    <  1:502285/251‑1 (MQ=255)    cGGGCAGATAACGCCGGAGAGGGGGGGATCCTGACGTTGCTGTCGCTTGCCGGGAGTAATACGTCGGCGCGAACCACATCAATGCTGGCG‑ATTATGGGGCTAATCGGCGGCAGCTTTTTCTATGGTGCAGTCGTCATAACACCAGCTATTTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCCGGCTACATGGATAGTCACCCTCTCAATTATCGTTCTCACTTTATTATCTATGAt                                                                                                                                                                                                                                                 >  1:432147/1‑251 (MQ=255)              aCGCCGGTGAGGTGGGGATCCTGACGTTGATGTCGCTTGCCGGGCGTAATACGTCGGCGCGAACCACATCAATGCTGGTG‑ATTATGGGGCTAATCGGCGGCAGCTTTTTCTATGGTGAAGTCGTCATAACACCCGCTATTTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATACCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATCTATGATTCAAAAACAt                                                                                                                                                                                                                                       <  1:223483/251‑1 (MQ=255)                    gTGAGGGGGGGATCCTGACGTTGATGTCGCTTGCCGGGCGTAATACGTCGGCGCGAACCACATCAATGCTGGTG‑ATTATGGGGCTAATCGGCGGCAGCTTTTTCTATGGTGAAGTCGTCATAACACCCGCTATTTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATACCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATCTATGATTCAAAAACATGGCACg                                                                                                                                                                                                                                 >  1:145772/1‑251 (MQ=255)                      gAGGGGGGGATCCTGACGTTGATGTCGCTTGCCGGGCGTAATACGTCGGCGCGAACCACATCAATGCTGGTG‑ATTATGGGGCTAATCGGCGGCAGCTTTTTCTATGGTGAAGTCGTCATAACACCCGCTATTTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATACCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATCTATGATTCAAAAACATGGCACGGc                                                                                                                                                                                                                               <  1:271311/251‑1 (MQ=255)                        gggggggACCCTGACGTTGAGGTCGCTGGCCAGGCGTAATACGTCGGCGCGAACCACATCAATGCTGGTG‑ATTATGGGGCTAGTCGGCGGCAGCTTTTTCTATGGTGAAGTCGTCATAACACCCGCTATTTCGGTGATGTCAGCCAGTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATACCTGGATAGTTCCCCTCTCAATGATCGTTCTCACTTTATTATCTATGATTCAAAAACATGGCACGGCTa                                                                                                                                                                                                                             <  2:481984/251‑1 (MQ=255)                          gggggATCCTGACGTTTATGTCGCTTGCCGGGCGTAATACGTCGGCGCTAACCACATCAATGCTGGTG‑ATTATGGGGCTAATCGGCGGCAGCTTTTTCTATGGTGAAGTCGTCATAACACCCGCTATTTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCATCTTGATACCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATCTATGATTCAAAAACATGGCACGGCTATg                                                                                                                                                                                                                           <  2:399940/251‑1 (MQ=255)                               aTCCTGACGTTGATGTCGCGTGCCGGGCGTAATACGTCGGCGCGAACCACATCAATGCTGGTG‑ATTATGGGGCTAATCGGCGGCAGCTTTTTCTATGGTGAAGTCGTCATAACACCCGCTATTTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATACCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATCTATGATTCAAAAACATGGCACGGCTATGGTCgg                                                                                                                                                                                                                      <  2:82011/251‑1 (MQ=255)                                    taCGTTGATGTCGCTTGCCTGTCGTAATACTTCGGCGCGAACCACATCAATTC‑GGTGGATAAAGGGGCTAACCGGCGGCATCTTTTTCTATGGTGAAGTCGTCATAACACCCGCTATGTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATACCTGGATAGTTCCCCTCTCAATTATCGTTCTCACCAGATTATCTATGATTCAAAAACATGGCACGGCTATGGTCGGTaa                                                                                                                                                                                                                   <  2:313289/248‑1 (MQ=255)                                       gTTGATGTCGCTTGCCGGGCGTAATACGTCGGCGCGAACCACATCAATGCTGGTG‑ATTATGGGGCTAATCGGCGGCAGCTTTTTCTATGGTGAAGTCGTCATAACACCCGCTATTTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATACCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATCTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGt                                                                                                                                                                                                              >  1:169238/1‑251 (MQ=255)                                                       gggCGTAATACGTCGGCGCGAACCACATCAATGCTGGTG‑ATTATGGGGCTAATCGGCGGCAGCTTTTTCTATGGTGAAGTCGTCATAACACCCGCTATTTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATACCTTGATAGTTCCCATCTCAATTATCGTTCTCACTTTATTATCTATGATTCATAAACATGGCACGGCTCTGGTCGGTAAGCTGCTTGCGCCGATCATGCt                                                                                                                                                                                              >  1:58450/1‑251 (MQ=255)                                                             aaTACGTCGGCGCGAACCACATCAATGCTGGTG‑ATTATGGGGCTAATCGGCGGCAGCTTTTTCTATGGTGAAGTCGTCATAACACCCGCTATTTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATACCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATCTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCt                                                                                                                                                                                         <  1:132438/250‑1 (MQ=255)                                                             aaTACGTCGGCGCGAACCACATCAAGGCTGGTG‑ATTATGGGGCTAATCGGCGGCAGCTTTTTCTATGGTGAAGTCGTCATAACACCCGCTATTTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATACCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATCTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTg                                                                                                                                                                                        <  2:145772/251‑1 (MQ=255)                                                              aTACGTCGGCGCGAACCAAATCAATGCTGGTG‑ATTATGGGGCTAATCGGCGGCAGCTTTTTCTATGGTGAAGTAGTCGTAACACCCGCTATTTCGGGGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATACCTGGGTCGTTCCCCTCTCAATTATCGTTCTCACTTTGTTATCTATGCTTCAAAAACATGGCACGACTATGGTCGGTAAGCGGTTTGCGCCGATCCTGCTGGCCTgg                                                                                                                                                                                       >  1:94868/1‑251 (MQ=255)                                                                     ggCGCGAACCACATCAATTCTGGTG‑ATTATGGGGCTAATCGGCGGCAGCTTTTTCTATGGTGAAGTCGTCATAACACCCGCTATTTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATACCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATCTATGATTCAAAAACATGGCTCGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGa                                                                                                                                                                                <  2:448474/251‑1 (MQ=255)                                                                                       gCTGGTG‑ATTATGGGGCTAATCGGCGGCAGCTTTTTCTATGGTGAAGTCGTCATAACACCCGCTGTTTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATACCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATCTATGATTCCAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGt                                                                                                                                                              >  2:396676/1‑251 (MQ=255)                                                                                           gTG‑ATTATGGGGCTAATCGGCGGCAGCTTTTTCTATGGTGAAGTCGTCATAACACCCGCTATTTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATACCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATCTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACg                                                                                                                                                          >  1:348968/1‑251 (MQ=255)                                                                                                ttATGGGGCTAATCGGCGGCAGCTTTTTCTATGGTGAAGTCGTCATAACACCCGCTATTTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATACCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATCTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGc                                                                                                                                                      >  1:272441/1‑251 (MQ=255)                                                                                                ttATGGGGCTAAGCGGCGGCAGCTTTTTCTATGGTGAAGTCGGCATAACACCCGCTATTTCGGGGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATACCTGGATAGTTCCCCGCTCAATTATCGTTCTCACTTTATTATCTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGAGCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAg                                                                                                                                                       <  2:497182/250‑1 (MQ=255)                                                                                                             cggcggTAGCGTTTCCTATGGTGAAGTCGTCATAACACCCGCTATTTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTTGCCCCTCAGCTGGATACCTGGATAGTTCCCCTCGCAATTATCGTTCTCACTTTATTATCTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCGGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAAcc                                                                                                                                         <  1:291842/251‑1 (MQ=255)                                                                                                              ggcggcAGCTTTTTCTATGGTGAAGTCGTCATAACACCCGCTATTTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATACCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATCTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATTCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAAccc                                                                                                                                        <  1:317165/251‑1 (MQ=255)                                                                                                                  gcAGCTTTTTCTATGGTGAAGTCGTCATAACACCCGCTATTTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATACCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATCTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGaa                                                                                                                                    <  2:498849/251‑1 (MQ=255)                                                                                                                    aGCTTTTTCTATGGTGAAGTCGTCATAACACCCGCTAGTTCGGTGAGGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATACCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATCTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGt                                                                                                                                  <  2:82163/251‑1 (MQ=255)                                                                                                                           tctttggggAAGTCGTAATATCACCCGCTATTGCTGTGTTGTCAGTAATGGCAGGCCATGAAATCTTCGCCCCGAAGCTGGAGACCTGGATAGTTCCCCTCCTAATGATCGTTCTCACCTTATTATCAATAATTCAAAAACATGGCTCTGCTATGGTCGGGAAGATGTTTGCGCTGATCATGCTGACCTTGTTTTTGATGCTGGCAGGACTGGGTTTACGTAGCATTATTGCTAACCCGGAAGtgctgtc                                                                                                                            <  2:385434/242‑3 (MQ=255)                                                                                                                                ggTGAAGTCGTCCTAACACCCGCTATTTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATACCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATCTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCt                                                                                                                      >  1:125414/1‑251 (MQ=255)                                                                                                                                     aGTCGTCATAACACCCGCTATTTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATACCTGGATAGTTCCCCTCTCAATTATCGTGCTCACTTTATTATCTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGATAACCCGGCAGTGCTGCATGCGCTGAATc                                                                                                                 >  2:262491/1‑251 (MQ=255)                                                                                                                                     aGTCGTAATAACACCCGCTATTTAGGTGATGTCAGCCCATGCAGGTCTGGAAATCGTCGCCCCGCAGCTGGATACCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATCTCTGATTCCAAAACATGGCACGGCTATGGTCGGAAAGCTATTTGCGCCGATCATTCTGCCCTGTTTTTTGTTTCTGGCAGGACTGGGGTTACGTAGCATTAGTGGTAACCCGGAAGggctggcagtgctgaatc                                                                                                                 >  2:369586/1‑238 (MQ=255)                                                                                                                                           cATAACTCCCGCTATTTCGGTGATGTCAGCCATTGAAGTGCTGGAAATCGTCGCCCCGCAGCGGGATACCTGGAGAGTTCCCCTATCAATTATCGTTCTCACTTTATTATCTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCATGACTGGGGTTACGTAGCATTATTGCTAACCCGTAAGTGCTGCATGCGCTGAATCCAAtg                                                                                                            <  1:111559/250‑1 (MQ=255)                                                                                                                                              aaCACCCGCTATTTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATACCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATCTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTggg                                                                                                        >  2:50194/1‑251 (MQ=255)                                                                                                                                                cacCCGCTATTTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATACCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATCTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCg                                                                                                      >  2:385314/1‑251 (MQ=255)                                                                                                                                                    cGCTACTTCGGTGATGTCAGCCATTGATGGTCTGGAAATCGTCGCCCCGCAGCTGGATACCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATCTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGGGCTGCATGCGCTGAATCCAATGTGGGCTGTGc                                                                                                  <  2:348968/251‑1 (MQ=255)                                                                                                                                                                                   cTGGAAATCGTCGCCCCGCAGCTGGATACCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATCTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCCTGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCttt                                                                   >  2:148507/1‑251 (MQ=255)                                                                                                                                                                                    tGGAAATCGTCGCCCCGCAGCTGGATACCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATCTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCtttt                                                                  >  1:39133/1‑251 (MQ=255)                                                                                                                                                                                            gtcgCCCCGCAGCTGGATACCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATCTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCAtt                                                          <  2:253339/251‑1 (MQ=255)                                                                                                                                                                                                ccccGCAGCTGGATACCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATCTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATgc                                                                                                                         <  2:128217/184‑1 (MQ=255)                                                                                                                                                                                                ccccGCAGCTGGATACCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATCTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATgc                                                                                                                         >  1:128217/1‑184 (MQ=255)                                                                                                                                                                                                          tgATACCTGGTTAATTCCCCCCTCAATTATCGGTCTCTCTTTATTGTCTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGGCCTGGTTTTTGATTCTGGCCGGACTGGGGTTACTTAGCATTATTGCTAACCCGGAAGTTCTGCATGCGCTGAATCCAATGTTGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTGCTTTGATTGCATTAGGGGCAg‑tggtg                                            <  2:483337/249‑1 (MQ=255)                                                                                                                                                                                                            aTACCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATCTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAG‑TGGtgttg                                         <  1:175772/251‑1 (MQ=255)                                                                                                                                                                                                                          ccccTCTCAATTATCGTTCTCACTTTATTATCTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAG‑TGGTGTTGTCGATTACGGGGGt                           <  1:271086/251‑1 (MQ=255)                                                                                                                                                                                                                                 cAATTATCGTTCTCACTTTATGATCTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAG‑TGGTGTTGTCGATTACGGGGGTCGAg                       <  2:39133/248‑1 (MQ=255)                                                                                                                                                                                                                                   aTTATCGTTCTCACTTTATTATCTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAG‑TGGTGTTGTCGATTACGGGGGTCGAGGCGCt                  <  1:353494/251‑1 (MQ=255)                                                                                                                                                                                                                                        cGTTCTCACTTTATTATCTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGATCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGTAAGTGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAG‑TGTGGTGGTCGATTACGGGGGTCGAGGCGCTGTAt              <  1:440411/250‑1 (MQ=255)                                                                                                                                                                                                                                             tcACTTTATTATCTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAG‑TGGTGTTGTCGATTACGGGGGTCGAGGCGCTGTATGCTGat        >  1:214750/1‑251 (MQ=255)                                                                                                                                                                                                                                                  ttattaTCTATGGTTCAAAAACATGGCACGGCTATGGTGGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGATGAATGCGCTGAATCAAATGTGGGAGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGG‑AGATAGAGTTGTCGATTACGGGAGTCGGGGAGCGGTAGGCTGatatagg   >  2:290962/1‑248 (MQ=255)                                                                                                                                                                                                                                                   tattatCTATGATTCAAAAACCTGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCCTGCGCTGACTCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAT‑GGGTGTTGTCGATTCCGGGGGTCGAGGCGCTGTATGCTGATATgggg  >  2:490763/1‑251 (MQ=255)                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                      ATGCGGGCAGATAACGCCGGTGAGGGGGGGATCCTGACGTTGATGTCGCTTGCCGGGCGTAATACGTCGGCGCGAACCACATCAATGCTGGTG‑ATTATGGGGCTAATCGGCGGCAGCTTTTTCTATGGTGAAGTCGTCATAACACCCGCTATTTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATACCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATTTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAG‑TGGTGTTGTCGATTACGGGGGTCGAGGCGCTGTATGCTGATATGGGG  >  NC_000913/3931532‑3932023

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑