| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,773,176 | C→T | S299F (TCT→TTT) | mtlA → | mannitol‑specific PTS enzyme IICBA component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,773,176 | 0 | C | T | 98.2% | 165.3 / ‑4.7 | 56 | S299F (TCT→TTT) | mtlA | mannitol‑specific PTS enzyme IICBA component |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base T (27/27); total (29/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.35e-01 | |||||||||||
CCAGGTATGGGCGTGCTGCTGGCGTACATGTTCTTTGGTCGTGGTAGCGCTAAACAGTCTGCGGGCGGTGCGGCAATCATCCACTTCCTGGGGGGTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGGGTTCTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCT‑AACATCGCG‑GGTGTGTGTGCGGCGATGGCT‑GTCTCCTTCGTTGTCTCTGCTATTTTGCTGAAAACCAGCAAAGTGAAAGAAGAAGATGATATTGAAGCAGCAACTCGTCGTATGCAGGACATGAAAGCTGAGTCTAAAGGCGCATCTCCGCTGTCTGCTGGCGATGTGACTAACGACCTGAGCCACGTACGTA > NC_000913/3772947‑3773415 | ccaggtATGGGCGTGCTGCTGGCGTACATGTTCTTTGGTCGTGGTAGCGCTAAACAGTCTGCGGGCGGTGCGGCAATCATCCACTTCCTGGGGGGTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGAt < 1:178192/250‑1 (MQ=255) caggtATGGGCGTGCTGCTGGCGTACATGTTCTTTGGTCGTGGTAGCGCTAAACAGTCTGCGGGCGGTGCGGCAATCATCCACTTCCTGGGGGGTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTg > 1:72357/1‑236 (MQ=255) caggtATGGGCGTGCTGCGGGCGTACATGTTCTTTGGCCGTGGTAGCGCTAAACAGTCTGCGGGCGGTGCGGCAATCATCCACTTCCTGGGGGGTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTg < 2:72357/236‑1 (MQ=255) aTGGGTGTGCTGCTGGCGTACATGTTCTTTGGTCGTGGTAGCGCTAATCATTCTGCGGGCGGTGCGGCAATCATCCACTTCCGGGGGGGTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGTTTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCa < 1:480127/251‑1 (MQ=255) gctgGCGTACATTTTCGTTGGTCGTGTTATCGTGAAACAGTCTGCGGGCGGTGCGGTAATAATCCACTTCCTGGGGTGTATCCACGAAATCTTCTTCCCGTATGTGCTGATGAATCCGCTTCTGATCCTCGCAGGCATCCTCGGCGGTATGACTGGCGTGTGCACGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGTCACCAAAAGGTGCttc < 2:117506/251‑2 (MQ=255) gctgGCGTACATGTTCTTTGGTCGTGGTAGCGCTAAACAGTCGGCGGGCGGTGCGGCAATCATCCACTTCCTGGGGGGGATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGTGGTATGTCTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACGCCAAAAGGTGCTTa < 1:201683/251‑1 (MQ=255) ctgGCGTACATGTTCTTTGGTCGTGGTAGCGCTAAACAGTCTGCGGGCGGTGCGGCAATCATCCACTTCCTGGGGGGTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGCCACCAAAAGGTGCTTa > 1:329337/1‑250 (MQ=255) ggCGTACATGTTCTTTGGTCGTGGTAGCGCTAAACAGTCTGCGGGCGGTGCGGCAATCATCCACTTCCTGGGGGGTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTa < 2:329337/248‑1 (MQ=255) gCGTACATGTTCTTTTGTCTTGGTAGCGCTAAACAGTCTGCGGGCGGTGCGGCAATCATCCACTTCCTGGGGGGTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAATGTGCTTACTTc < 2:91827/251‑1 (MQ=255) aTGTTCTTTGGTCGGTGTATCGCTACACAGTCTGCGGGCGGTGCGGCAATCATCCACTTCCTGGGGGGTATCCACGAAATCTACTGCCCGTATGTGCTGATGAATCCGCGTCTGGTCCTCGCAGTCATCCTCGTCGGTATGACTGGCGTGTTCACGCTGACGATCCTGGGCGGTGGTCTGGTTCCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCT‑AAc < 2:494561/251‑1 (MQ=255) ttCTTTGGTCGTGGTAGCGCTAAACAGTCTGCGGGAGGTGCGGCAATCATCCACTTCCTGGGGGGTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTCTCCTGGGCGGTGGTCTGGTTTCTCCGGCATTTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCT‑ACCATc > 2:446777/1‑251 (MQ=255) gctgtGGTCGTGGTAGCGCGCAACAGTCTGCGGGCGCTGCGGCAATCATCCACGTCCTGGGGGGTATCCACGAAAGCTACTTCCCGTATGTGCTGATGAATCCGCGGCTGATCCTCGAAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCAGCTCCGG‑TTTTATTCTTGCTGTACTGGCGATGTCACCAAAAGGTGCTTACTTCGCT‑AACATc < 2:178284/246‑1 (MQ=255) ttGGTCGTGGTAGCGCTAAACAGTCTGCGGGCGGTGCGGCAATCATCCACTTCCTGGGGGGTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCT‑AACATcg < 1:420648/248‑1 (MQ=255) gTGGTAGCGCTAAACAGTCTGCGGGCGGTGCGGCAATCATCCACTTCCTGGGGGGTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCCCGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCT‑AACATTGCT‑Ggtgggg > 2:178869/1‑248 (MQ=255) ggtagcgctaacgagctggcgggcggTGCGGCAATCATCCTCTGCCTGGTGGGTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATGCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCTGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCGTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCT‑AACATCGCG‑Ggtgtgtgt < 2:376936/233‑1 (MQ=255) tAGCGCTAAACAGTCTGCGGGCGGTGCGGCAATCATCCACTTCCTGGGGGGTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGCCTGGCGTGTTCACGCTGACTATCCTGGGCGGAGGGCTGGGTTCTCCGGCATCACCGG‑TTTTATCCTTGCCGTACCGGCGATTCAAACAAATGGGGCTTAAATCGCT‑AACCTCGCG‑GGggggtgtgg > 2:274145/1‑250 (MQ=255) aGCGCTAAACAGTCTGCTGGCGGTGCGGCAATCATCCACTTCCTGGGGGGTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCTG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCT‑AACCTCGCG‑GGTGTGGGTgcg < 2:96356/251‑1 (MQ=255) tAAACAGTCTGCGGGCGGTGCGGCAATCATCCACTTCCTGGGGGGTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGGGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGGGCTTACTTCGCT‑AACATCGCG‑GGGGTGTGTgcggcggt > 1:25476/1‑249 (MQ=255) aaCAGTCTGCGGGCGGTGCGGCAATCATCCACTTCCTGGGGGGTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACCATCCTGGGAGGGGGTCTGGTTTCTCCCGCATCTCCGG‑TTTTATCCTTGCTGTACTGGAGATGACACCAAAAGGGGCTTACTTCGCTTAAC‑TCGCGCGgtgtgttgccgctgatg > 2:449930/1‑240 (MQ=255) cAGTCTGCGGGCGGTGCGGCAATCATCCACTTCCTGGGGGGTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGGCTGGTTTCTCCGGCATATCCGG‑TTTTATTCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTTCTTCGCT‑AACATCGCG‑GGGGTGTGTGCGGCGGTGGCt > 2:72822/1‑251 (MQ=255) ccgctctaaacacccACTTCCTGGGGGGTATCCCCGAAATCTACTTCCCGGATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCT‑AACATCGCG‑GGTGTGTGTGCGGCGATGGCT‑GTCTCCTTCGTTg < 2:501937/238‑1 (MQ=255) aaTCATCCACTTCCTGGGGGGTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCT‑AACATCGCG‑GGTGTGTGTGCGGCGATGGCT‑GTCTCCTTCGTTg > 1:501937/1‑244 (MQ=255) tCCTGGGGGGTATCCACGAAATCTACTTCCCGTATGTGCTGATTAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGTCTGGCGTTTTCACGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTTCTTCGCT‑AACATCGCG‑GGTGTGTGTGCGTCGATGGCT‑GTCTCCTTCGTTGTCTCTGCTATGTTGCt < 1:366755/249‑1 (MQ=255) tCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCCCT‑AACCTCGCG‑GGGGTGTGTGCGGCGATGGCT‑GTCTCCTTTGTTGTCTCTGCTTTTTTTCTGCAAACCAGcca > 2:313284/1‑247 (MQ=255) ccACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTATGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACGATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTCCTGGCGATGACACCAAAAGGTGCTTACTTCGCT‑AACATCGCG‑GGTGTGTGTGCGGCGATGGCT‑GTCTCCTTCGTTGTCTCTGCTATTTTGCTGAAAACCAGCAAAGt < 1:94637/251‑1 (MQ=255) tCTACTTACAGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCATCGGCGGACTGACTGGCGTGTTCCCGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTTCTTGCGATGACACCAAAAGGTGCTTTCTTCGCT‑AAAATCGCG‑GGTGTGTGTGCGGCGATGGCT‑GTCTCCCTCGTTGTATCTGCTATTTTTTTGAAAACCAGCAAAGTGAaaga > 2:464707/1‑249 (MQ=255) tACTTCCCGTATGTGCTGATGAATCCACGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACCCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGTTGAAACCAAAAGGTGCTTACTTCGCT‑AACATCGCG‑GGTGTGTGTGCGGCGATGGCT‑GTATCCTTCGTTGTCTCTTCTATTTTTCTGAAAACCAGCCAAGGGAaagaaga > 2:324796/1‑250 (MQ=255) aCTTCCCGTATGTGCTGATGAATCCGCGTCTGCTCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGTGCGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCT‑AACATCGCG‑GGTGTGTGTGCGGCGATGGCT‑GTCTCCTACGTTGTCTCTGCTATTTTGCTGAAAACCAGCAAAGTGAaagaa < 2:415251/247‑1 (MQ=255) ccggattggCTGATTAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCT‑AACATCGCG‑GGTGTGTGTGCGGCGATGGCT‑GTCTCCTTCGTTGTCTCTGCTATTTTGCTGAAAACCAGCAAAGTGAAAGAAGAAgatgat < 2:25476/243‑1 (MQ=255) atgaATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGGGTTCTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCC‑AACATCGCG‑GGTGGGTGTGCGGCGATGGCT‑GTCTCCTTCGTTGTCTCTGCTATTTTGCTGAAAACCAGCAAAGTGAAAGAAGAAGATGATATTGAagcagc > 1:204590/1‑251 (MQ=255) aTCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCCCGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCCTC‑CCCGGTTTTATCCTTTCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCT‑AACATCCCG‑GGTGGGTGTGCGGCGATGGCT‑GTCCCCTTCGTTGTCTCTGCTATTTTGCTGAAAACCAGCCAAGTGAAaaaaggagaagtatttgaagcacacactc > 2:389947/1‑231 (MQ=255) aTCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCCCGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGAATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCT‑AACCTCCCG‑GGGGGTTGGGCGGCGGTGGCT‑GTCTCCTTCGTTTGCTCTGCTATTTTGCTGGAAACCAGCCAAGTGAAAGAAGACGATGGTATTGAAGCAGCAAcgc > 2:396553/1‑249 (MQ=255) ccGCGTCTGAGCCTCTCAGTCATCCTCGTGGGTTTGTCTTGCTTGTTCACGCTGACTATCCTGGGCGGTGGTCTGGTTTTTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCT‑AACATCGCT‑GGTGTGTGTGCGGCGATGGCT‑GTCTCCTTCGTTGTCTTTGCTATTTTGCTGAAAACCAGCAAAGTGAAAGAAGAAGATGATATTGAAGCAGCAACtcgt < 2:293719/251‑1 (MQ=255) gcgTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCT‑AACATCGCG‑GGGGTGTGTGCGGCGATGGCT‑GTCTCCTTCGTTGTCTCTGCTATTTTGCTGCAAACCAGACAAGTGTAAGAAGAAGATGATATTGAAGCAGCAACtcgtcg > 1:240917/1‑251 (MQ=255) tCTGATCCTCGAAGTCATCCTCGGCGGTATGACTTGCGTGTTAACGCTGACTATCCTGGGAGGCGGTCAGGGTTATACGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCT‑AACATCTCG‑GGGGTGTGGGCGGGGGGGGCTTGT‑TTCTTCGCTGTTTCTGCTATTTTGCTGGAAACCCGCAAAATGAAAGAAGATGTTGATTTTGAAAAAGCAACACGTGGTAt > 2:312551/1‑251 (MQ=255) gATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGGCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCT‑AACATCGCG‑GGTGTGTGTGCGGCGATGGCT‑GTCTCCTTCGTTGTCTCTGCTATTTTGCTGAAAACACGCAAAGTGAAAGAAGAAGATGATATTGAAGCAGCAACTCATCGTATGc > 1:494761/1‑250 (MQ=255) ccgcGCAGTCATCCTCGGCGGTATGACTGGCGTGGTCACGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCT‑AACATCGCG‑GGTGTGTGTGCGGCGATGGCT‑GTCTCCTGCGTTGTCTCTGCTATTTTGCTGAAAACCAGCAAAGTGAAAGAAGAAGATGATATTGAAGCAGCAACTCGTCGTATGCAGGa < 2:357016/248‑1 (MQ=255) ccTCGCAGGCGTCCTCGGCGGTATGACTGGCGTGGTCACGCTTACTAGCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCCTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCT‑AACATCGCG‑GGTGTGTGTGCGGCGATGGCT‑GTCTCCTTCGTTGTCTCTGCTATATTGCTGAAAACCAGCAAAGTGAAAGAAGAAGATGATATTGAAGCAGCACCTCGTCGTACGCAGGa < 2:333272/251‑1 (MQ=255) cTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTTCTGTACTGGCGATGACACCCAACGGTGCTTACTTCGCT‑AACATCGCG‑GGTGTGTGTGCGGCGAAGGCT‑GTCTCCTTCGTTGTCTCTGCGATTTTTCTGAAAACCAGCAAAGTGAAAGAAGAAGATGATATTGAAGCAGCAACTCGGCGTATGCAGGa > 2:306883/1‑250 (MQ=255) cGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGGGTT‑TATCCTTGCTGTACTGGCGATGACACCCAAAGGTGCTTACTTCGCT‑AACATCGCG‑GGGGTGTGGGCGGCGATGGCT‑GTCTCCTTCGTTGTCTCTGCTATTTTGCTGGAAACCAGCAACGGGAAAGCAGAAGATGATATTTAAGCAGCAACTCGTCGTATGccgggcat > 2:502166/1‑244 (MQ=255) cGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCT‑AACATCGCG‑GGTGTGTGTGCGGCGATGGCT‑GTCTCCTTCGTTGTCTCTGCTATTTTGCTGAAAACCAGCAAAGTGAAAGAAGAAGATGATATTGAAGCAGCAACTCGTCGTATGCAGGAc < 2:233667/249‑1 (MQ=255) gCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGTCTGGGTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCT‑AACATCGCG‑GGTGTGTGTGCGGCGATGGCT‑GTCTCCTTCGTTGTCTCTGCTATTTTGCTGAAAACCAGCAAAGTGAAAGAAGAAGATGATATTGAAGCAGCAACTCGTCGTATGCAGGACATg > 2:475232/1‑251 (MQ=255) aTCCTCGGCGATATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCT‑AACATCGCG‑GGTGTGTGTGCGGCGATGGCT‑GTCTCCTTCGTTGTCTCTGCTATTTTGCTGAAAACCAGCAAAGTGAAAGAAGAAGATGATATTGAAGCAGCAACTCGTCGTATGCAGGACATGAAAGCt > 1:503917/1‑251 (MQ=255) ccggcgtTATGACTGGCGTGTGCACGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCT‑AACATCGCG‑GGTGTGTGTGCGGCGATGGCT‑GTCTCCTTCGTTGTCTCTGCTATTTTGCTGAAAACCAGCAAAGTGAAAGAAGAAGATGATATTGAAGCAGCAACTCGTCGTATGCAGGACATGAAAGCTTAGt < 1:222868/250‑1 (MQ=255) ggcggTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCT‑AACATCGCG‑GGGGTGTGTGCGGCGATGGCT‑GTCTCCTTCGTTGTCTCTGCTATTTTGCTGAAAACCAGCAAAGTGAAAGAAGAAGATGATATTGAAGCAGCAACTCGTCGTATGCAGGACATGAAAGCTGAGTCt > 1:94487/1‑251 (MQ=255) ggTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCT‑AACATCGCG‑GGTGTGTGTGCGGCGATGGCT‑GTCTCCTTCGTTGTCTCTGCTATTTTGCTGAAAACCCGCAAAGGGAAAGAAGAAGATGATATTGACGCAGCAACTCGTCGTATGCAGGACATGAAAACTGAGTCTaa > 2:398456/1‑250 (MQ=255) cACGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCT‑AACATCGCG‑GGTGTGTGTGCGGCGATGGCT‑GTCTCCTTCGTTGTCTCTGCTATTTTGCTGAAAACCAGCAAAGTGAAAGAAGAAGATGATATTGAAGCAGCAACTCGTCGTATGCAGGACATGAAAGCTGAGTCTAAAGGCGCATCTCCGCTGTc < 1:72822/251‑1 (MQ=255) aCTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCT‑AACATCGCG‑GGTGTGTGTGCGGCGATGGCT‑GTCTCCTTCGTTGTCTCTGCTATTTTGCTGAAAACCAGCAAAGTGAAAGAAGAAGATGATATTGAAGCAGCAACTCGTCGTATGCAGGACATGAAAGCTGAGTCTAAAGGCGCATCTCCGCTGTCTGCTGGc > 1:95208/1‑251 (MQ=255) tGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGCTAACACCAAAAGGTGCTTACTTCGCT‑AACATCGCG‑GGTGTGTGTGCGGCGATGGCT‑GTCTCCTTCGTTGTCTCTGCTCTTTTGCTGAAAACCAGCAAAGTGAAAGAAGAAGATGCTATTGAAGCAGCAACTCGTCGTATGCAGGCCATGAAAGCTGAGTCTACAGGCGCCTCTCCGCTGTCTGCTGGCGAtgtg > 1:330024/1‑250 (MQ=255) tGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCT‑AACATCGCG‑GGTGTGTGTGCGGCGATGGCT‑GTCTCCTTCGTTGTCTCTGCTATTTTGCTGAAAACCAGCAAAGTGAAAGAAGAAGATGATATTGAAGCAGCAACTCGTCGTATGCAGGACATGAAAGCTGAGTCTAAAGGCGCATCTCCGCTGTCTGCTGGCGAtgtg > 1:155560/1‑250 (MQ=255) tggtCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCT‑AACATCGCG‑GGTGTGTGTGCGGCGATGGCT‑GTCTCCTTCGTTGTCTCTGCTATTTTGCTGAAAACCAGCAAAGTGa < 1:287655/151‑1 (MQ=255) tggtCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCT‑AACATCGCG‑GGTGTGTGTGCGGCGATGGCT‑GTCTCCTTCGTTGTCTCTGCTATTTTGCTGAAAACCAGCAAAGTGa > 2:287655/1‑151 (MQ=255) ggTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCT‑AACATCGCG‑GGTGTGTGTGCGGCGATGGCT‑GTCTCCTTCGTTGTCTCTGCTATTTTGCTGAAAACCAGCAAAGTGAAAGAAGAAGATGATATTGAAGCAGCAACTCGTCGTATGCAGGACATGAAAGCTGAGTCTAAAGGCGCATCTCCGCTGTCTGCTGGCGATGTGACTAACGACCTg < 1:398456/249‑1 (MQ=255) gTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCT‑AACATCGCG‑GGTGTGTGTGCGGCGATGGCT‑GTCTCCTTCGTTGTCTCTGCTATTTTGCTGAAAACCAGCAAAGTGAAAGAAGAAGATGATATTGAAGCAGCAACTCGTCGTATGCAGGACATGAAAGCTGAGTCTAAAGGCGCATCTCCGCTGTCTGCTGGCGATGTGACTAACGACCTGAGc < 2:115644/251‑1 (MQ=255) cGGCCTCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCT‑AACATCGCG‑GGTGTGGGTGCGGCGATGGCT‑GTCTCCTTCGTTGTCTCTGCTATTTTGCTGAAAACCAGCAAAGTGAAAGAAGAAGATGATATTGAAGCAGCAACTCGTCGTATGCAGGACATGAAAGCTGAGTCTAAAGGCGCATCTCCGCTGTCTGCTGGCGATGTGACTAACGACCTGAGCCacgtac < 2:94487/251‑1 (MQ=255) aTCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCT‑AACATCGCG‑GGTGTGTGTGCGGCGATGGCT‑GTCTCCTTCGTTGTCTCTGCTATTTTGCTGAAAACCAGCAAAGTGAAAGAAGAAGATGATATTGAAGCAGCAACTCGTCGTATGCAGGACATGAAAGCTGAGTCTAAAGGCGCATCTCCGCTGTCTGCTGGCGATGTGACTAACGACCTGAGCCAcgtacgta > 1:375021/1‑250 (MQ=255) | CCAGGTATGGGCGTGCTGCTGGCGTACATGTTCTTTGGTCGTGGTAGCGCTAAACAGTCTGCGGGCGGTGCGGCAATCATCCACTTCCTGGGGGGTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGGGTTCTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCT‑AACATCGCG‑GGTGTGTGTGCGGCGATGGCT‑GTCTCCTTCGTTGTCTCTGCTATTTTGCTGAAAACCAGCAAAGTGAAAGAAGAAGATGATATTGAAGCAGCAACTCGTCGTATGCAGGACATGAAAGCTGAGTCTAAAGGCGCATCTCCGCTGTCTGCTGGCGATGTGACTAACGACCTGAGCCACGTACGTA > NC_000913/3772947‑3773415 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |