Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,770,539	C→T	V281M (GTG→ATG)	yibH ←	inner membrane protein YibH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,770,539	0	C	T	100.0%	202.0 / NA	63	V281M (GTG→ATG)	yibH	inner membrane protein YibH
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (34/29);  total (34/29)

ACCGAAACATGGCTGAAATGGTCGGAGTAAACCGCCACCTGGGCGTAGATGCCGTCGGGTAAGGCATCGATATCATCGTTAGGGTCCAGTTCAATGGTTCCCAGCACACCGTCCGTGCCGGGCACGACCGTTAATGATTGCAATACCCCCTGCGCCTGATAAGAACCGCCTGGCACGACAGGTAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACACCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGAAGACCATCACCGGACGCAGCGGCAAGGCAGCTGCGTATGTACCTGGGCGGATCAGTACCTGAGTAACGTAGCCATTGCTCGGCGCGCGAATGACAGTCTGCTCAAGGTTATATTTTGCTTCAGTAAGTTGCGCTCTTAAGCTCACAATCTGCGATTGCTC  >  NC_000913/3770304‑3770785                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                       aCCGAAACATGGCTGAAATGGTCGGAGTAAACCGCCACCTGGGCGTAGATGCCGTCGGGTAAGGCATCGATATCATCGTTAGGGTCCAGTTCAATGGTTCCCAGCACACCGTCCGTGCCGGGCACGACCGTTAATGATTGCAATACCCCCTGCGCCTGATAAGAACCGCCTGGCACGACAGGTAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCatcatc                                                                                                                                                                                                                                         >  1:320029/1‑251 (MQ=255)            gCTGAAATGGTCGGCGTCAACCGCCACCTGGGCGTAGATGCCGCCGGGTAAGGCATCGGTATCATCGTTAGGGTCCAGTTCCATGGTTCCCAGCACACCGTCCGTGCCGGGCACGACCGTTAATGATTGAAATACCCCCTGCGCCTGATCAGAACCGCCTGGCCAGGCAGGTAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTc                                                                                                                                                                                                                               >  2:59147/1‑250 (MQ=255)                   tGGTCGGAGTAAACCGCCACCTGGGCGTAGATGCCGTCGGGTAAGGCATCGATATCATCGTTAGGGTCCAGTTCAATGGTTCCCAGCACACCGTCCGTGCCGGGCACGACCGTTAATGATTGCAATACCCCCTGCGCCTGATAAGAACCGCCTGGCACGACAGGTAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTa                                                                                                                                                                                                                        >  2:83707/1‑250 (MQ=255)                      tCGGAGTAAACCGCCACCTGGGCGTAGATGCCGTCGGGTAAGGCATCGATATCATCGTTAGGGTCCAGTTCAATGGTTCCCAGCACACCGTCCGTGCCGGGCACGACCGTTAATGATTGCAATACCCCCTGCGCCTGATAAGAACCGCCTGGCACGACAGGTAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAAc                                                                                                                                                                                                                      >  1:5051/1‑249 (MQ=255)                      tCGGAGAAAACCGCCCCCTGGGCGTAGATGCCGTCGGGTACGGCATCGATATCATCGTTAGGGTCCAGTTCAATGGCTCCCAGCACACCGTCCGTGCCGGGCACGACCGTTAATGATTGCCATACCCCCTGCGCCTGATAAGAACCGCCTGGCACGACAGGTAAAATACTCGTCAGTTTCCCGTGAAACACCTGCCCAGGCAGCGCGTTAAACATCACTTCCGCATCATCGCCCGGCTTCAGACGTAAc                                                                                                                                                                                                                      >  2:304485/1‑249 (MQ=255)                       cGGAGTAAACCGCCCCCTGGGCGTAGATGCCGTCGGGTAAGTCATCGATATCATCGTTAGGGTCCAGTTCAATGGTTCCCAGCACACCGTCCGTGCCGGGCACGACCGTTAATGATTGCAATACCCCCTGCGCCTGATAAGAACCGCCTGGCACGACAGGAAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAg                                                                                                                                                                                                                    <  1:76620/250‑1 (MQ=255)                             aaaCCCCCACCTGGGCGTAGATGCCGTCGGGTAAGGCCACGATTGCATCGTTAGGGTCCATTTCAATGGTTCCCAGCACACCGTCCGTGCCGGGCACGACCGTTAATGATTGCAATACCCCCTGCGCCTGATAAGAACCGCCTGGCACGACAGGTAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTGAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGttt                                                                                                                                                                                                             <  2:184393/251‑1 (MQ=255)                                 cGCCACCTGGGCGTAGATGCCGTCGGGTAAGGCATCGATATCATCGTTAGGGTCCAGTTCAATGGTTCCCAGCACACCGTCCGTGCCGGGCACGACCGTTAATGATTGCAATACCCCCTGCGCCTGATAAGAACCGCCTGGCACGACAGGTAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGcc                                                                                                                                                                                                         <  1:190761/251‑1 (MQ=255)                                 cGCCACCTGGGCGTAGAGGCCGTCGGGTAAGGCATCGATAGCATCGTTAGGGTCCATTTCAATGGTTCCCAGCACACCGTCCGTGCCGGGCACGACCGTTAATGATTGCAATACCCCCTGCGCCTGATAAGAACCGCCTGGCACGACAGGTAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGcc                                                                                                                                                                                                         <  2:160886/251‑1 (MQ=255)                                   ccACCTGGGCGTAGATGCCGTCGGGTAAGGCATCGATATCATCGTTAGGGTCCCGTTCAATGGTTCCCAGCACACCGTCCGTGCCGGGCACGACCGTTAATGATTGCAATACCCCCTGCGCCTGATAAGAACCGCCTGGCACGACAGGTAAAATACTAGTCAGTTTGCCGTGAAAAACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCg                                                                                                                                                                                                        >  2:425892/1‑250 (MQ=255)                                         gggCGTAGGTGCCGTGGGGAAATGCATCGATACTTTCGTTAGGGTCCATTTCAAGGTTTCCCAGCTCCCCGTCCGTGCCGGGCACGTCCGTTAATGATGGCAATACCCCCTGCGCCTGATAAGAACCGCCTGGCACGACAGGTAAAATACTAGGCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCAGCTCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAAtgg                                                                                                                                                                                                  <  2:297976/250‑3 (MQ=255)                                                gATGCCGTCGGGTAAGGCATCGATAGCATCGGTAGGGTCCAGTTCAATGGTTCCCAGCACACCGTCCGTGCCGGGCACGACCGTTAATGATTGCAATACCCCCTGCGCCTGATAAGAACCGCCTGGCACGACAGGTAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATGGGGCGACaa                                                                                                                                                                                          <  2:227602/251‑1 (MQ=255)                                                                      atatCATCGTTAGGGTCCAGTTCAATGGTTCCCAGCACACCGTCCGTGCCGGGCACGACCGTTAATGATTGCAATACCCCCTGCGCCTGATAAGAACCGCCTGGCACGACAGGTAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTTCTCggggtt                                                                                                                                                                    >  2:268326/1‑249 (MQ=255)                                                                      atatCATCGTTAGGGTCCAGTTCAATGGTTCCCAGCACACCGTCCGTGCCGGGCACGACCGTTAATGATTGCAATACCCCCTGCGCCTGATAAGAACCGCCTGGCACGACAGGTAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGAt                                                                                                                                                                    >  1:200131/1‑251 (MQ=255)                                                                          catcGTTAGGGTCCAGTTCAATGGTTCCCAGCACACCGTCCGTGCCGGGCACGACCGTTAATGATTGCAATACCCCCTGCGCCTGATAAGAACCGCCTGGCACGACAGGTAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGagga                                                                                                                                                                  >  1:95488/1‑246 (MQ=255)                                                                             cGTTAGGGTCCAGTTCAATGGTTCCCAGCACACCGTCCGTGCCGGGCACGACCGTTAATGATTGCAATACCCCCTGCGCCTGATAAGAACCGCCTGGCACGACAGGTAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGAAGAcc                                                                                                                                                             >  1:366134/1‑251 (MQ=255)                                                                                    gTCCAGTTCAATGGTTCCCAGCACACCGTCCGTGCCGGGCACGACCGTTAATGATTGCAATACCCCCTGCGCCTGATAAGAACCGCCTGGCACGACAGGTAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGAAGACCATCACCg                                                                                                                                                      <  1:127044/251‑1 (MQ=255)                                                                                     tCCAGTTCAATGGTTCCCAGCACACCGTCCGTGCCGGGCACGACCGTTAATGATTGCAATACCCCCTGCGCCTGATAAGAACCGCCTGGCACGACAGGTAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGAAGACCATCACCgg                                                                                                                                                     >  1:319916/1‑251 (MQ=255)                                                                                                      cAGCACACCGTCCGTGCCGGGCACGACCGTTAATGATTGCAATACCCCCTGCGCCTGATAAGAACCGCCTGGCACGACAGGTAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGtt                                                                                                                                                                                                                                 <  2:407981/158‑1 (MQ=255)                                                                                                      cAGCACACCGTCCGTGCCGGGCACGACCGTTAATGATTGCAATACCCCCTGCGCCTGATAAGAACCGCCTGGCACGACAGGTAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGtt                                                                                                                                                                                                                                 >  1:407981/1‑158 (MQ=255)                                                                                                       aGCACCCCGCCCGTGCCGGGCCCGACCGTTACTGATTGCAATACCCCCGGCGCCTGATAAGAACCGCCTGGCACGACAGGTAAAATACTAGTCAGTTTGCCGTGAAACCCCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAAGTTGCCGTTTTTGCTCGGGGATGAAGACCATCACCGGACGCAGCGGCAAGGCAGc                                                                                                                                   <  2:204562/251‑1 (MQ=255)                                                                                                          acacCGTCCGTGCCGGGCACGACCGTTAATGATTGCAATACCCCCTGCGCCTGATAAGAACCGCCTGGCACGACAGGTAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGAAGACCATCACCGGACGCAGCGGCAAGGCAGCTGc                                                                                                                                >  1:73578/1‑251 (MQ=255)                                                                                                                tccgtGCCGGGCACGACCGTTAATGATTGCAATACCCCCTGCGCCTGATAAGAACCGCCTGGCACGACAGGTAAAATCCTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGAAGACCATCCCCGGACGCAGCGGCAAGGCCGCTGCGTATGt                                                                                                                          >  2:456162/1‑251 (MQ=255)                                                                                                                tccgtGCCGGGCACGACCGTTAATGATTGCAATACCCCCTGCGCCTGATAAGAACCGCCTGGCACGACAGGTAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGAAGACCATCACCGGACGCAGCGGCAAGGCAGCTGCGTATGt                                                                                                                          >  1:86914/1‑251 (MQ=255)                                                                                                                  cgtGCCGGCCACGACCGTTTATGATTTCAATCCCCCCTGCGCCTGTTAAGAACCGCCTTGCACGACAGGTAAAATTCTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTTCTCGGGGATGAAGACCATCACCGGACGCAGCGGCAAGGTAGCTGCGTATGTAc                                                                                                                        <  1:373159/251‑1 (MQ=255)                                                                                                                      ccGGGCACGACCGTTAATGATTGCAATACCCCCTGCGCCTGATAAGAACCGCCTGGCACGACAGGTAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGAAGACCATCACCGGCCGCAGCGGCCAGGCAGCTGAGTATGTACCTgg                                                                                                                    >  2:11988/1‑251 (MQ=255)                                                                                                                      ccGGGCACGACCGTTAATGATTGCAATACCCCCTGCGCCTGATAAGAACCGCCTGGCACGACAGGTAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGAAGACCATCACCGGACGCAGCGGCAAGGCAGCTGCGTATGTACCTgg                                                                                                                    >  1:322096/1‑251 (MQ=255)                                                                                                                             cGACCGTTAATGATTGCAATACCCCCTGCGCCTGATAAGAACCGCCTGGCACGACAGGTAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGAAGACCATCACCGGACGCAGCGGCAAGGCAGCTGCGTATGTACCTGGGCGGATc                                                                                                             <  1:309337/251‑1 (MQ=255)                                                                                                                               aCCGTTAATGATTGCAATACCCCCTGCGCCTGATAAGAACCGCCTGGCACGACAGGTAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGAAGACCATCACCGGACGCAGCGGCAAGGCAGCTGCGTATGTACCTGGGCGGATc                                                                                                             >  1:316333/1‑249 (MQ=255)                                                                                                                                ccGTTAACGAGTGCAATCCCCCCGGCGCCTTATAAGAACCGCCTGGCACGACATGTAAAATACTAGTCAGTTATCCGTGAAACACCATCCCAGGTAGCTCGTTAATCATCACTGCCACATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTAGGAGACAATATGCCGTTTTTGCTCGTGGATGAAGACCATCACCGGACGCAGCGGCTAGGCATCTGCGTATGTACCTGGGCGGAGCAGt                                                                                                          <  2:402968/251‑1 (MQ=255)                                                                                                                                 cGTTACTGATTGCAATACCCCCTGCGCCTGATAAGAACCGCCTGGCACGACAGGTAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAGTTGGGCGCCAATTTGCCGTTTTTGCTCGGGGATGAAGACCATCACCGGACGCAGCGCCAAGCCAGCTGCGTATGTACCTGGACGGATCAGt                                                                                                          >  1:51854/1‑250 (MQ=255)                                                                                                                                 cGTTAATGATTGCAATACCCCCTGCGCCTGATAAGAACCGCCTGGCACGACAGGTAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGAAGACCATCACCGGACGCAGCGGCAAGGCAGCTGCGTATGTACCTGGGCGGATCAGTa                                                                                                         <  1:112823/251‑1 (MQ=255)                                                                                                                                  gtgaaTGATTGCAATACCACCTGCGCCTGATAAGACCCGCCTGGCACGACAGGTACAATACTAGTCAGTTTGCCGAGAAACCCCTGCCCAGGTAGCGCGTTAACCATCACTTCCGCATCATCGCCAGGGTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGCTAAAGACCATCACCGGACGCATCGGCAAGGCAGCGGCGAATGTACCTGGGCGGGTCAGTAc                                                                                                        >  2:400377/4‑251 (MQ=255)                                                                                                                                      aTGATTGCAATACCCCCTGCGCCTGATAAGAACCGCCTGGCACGACAGGTAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAAtt                                                                                                                                                                                                   >  2:301802/1‑156 (MQ=255)                                                                                                                                      aTGATTGCAATACCCCCTGCGCCTGATAAGAACCGCCTGGCACGACAGGTAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAAtt                                                                                                                                                                                                   <  1:301802/156‑1 (MQ=255)                                                                                                                                            gCAATACCCCCTGCGCCTGATAAGAACCGCCTGGCACGACAGGTAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGAAGACCATCACCGGACGCAGCGGCAAGGCAGCTGCGTATGTACCTGGGCGGATCAGTACCTGAGTAAc                                                                                               <  1:113527/250‑1 (MQ=255)                                                                                                                                                 aCCCCCTGCGCCTGATAAGAACCGCCTGGCACTACAGGTAAAATAAGTGTCAGTTTGCCGTGAAACCCCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCACCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGAAGACCATCACCGGACGCAGCGGCAAGGCAGCTGCGTATGTACCTGGGCGGATCAGTACCTGAGTAACGTAGc                                                                                          <  2:368243/250‑1 (MQ=255)                                                                                                                                                     ccTGCGCCTGATAAGAACCGCCTGGCACGACAGGTAAAATACTAGTCATTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGTGGATGAAGACCATCACCGGACGCAGCGGCAAGGCAGCTGCGTATGTACCTGGGCGGATCAGTACCTGAGTAACGTAGCCATTg                                                                                     <  1:459950/251‑1 (MQ=255)                                                                                                                                                         cgcCTGATAAGAACCGCCTGGCACGACAGGTAAAATACTAGCTAGTTTGCCGTGAAACCCCTGCCCAGGTAGCGCGTTAAACATCCCTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGAAGACCATCACCGGACGCAGCGGCAAGGCAGCTGCGTATGGACCTGGGCGGATCAGTACCTGAGTAACGTAGCCATTGCTCg                                                                                 <  2:294529/251‑1 (MQ=255)                                                                                                                                                          gcCTGATAAGAACCGCCTGGCACGACAGGTAAAATCCTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCCTCGCCAGGTTTCAGACGTCACAGCGAGTTTTGCCGCAATTGGGCGACAATTCGCCGTTTTTGCTCGGGGATGACGACCATCACCGGACGCAGCGGCAAGGCAGCTGCGTATGTACCTGGGCGGCTCAGTACCTGAGTAACGTAGCCATTGCTCgg                                                                                >  2:181592/1‑251 (MQ=255)                                                                                                                                                                  aGAACCGCCTGGCACGACAGGTAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGAAGACCATc                                                                                                                                                          >  2:298695/1‑169 (MQ=255)                                                                                                                                                                  aGAACCGCCTGGCACGACAGGTAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGAAGACCATc                                                                                                                                                          <  1:298695/169‑1 (MQ=255)                                                                                                                                                                   gAACCGCCTGGCACGACAGGTAAAATACTAGTCAGTTTGCCGTGAAACACCTACCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGAAGACCATCACCGGACGCAGCGGCAAGGCAGCTGCGTATGTACCTGGGCGGATCAGTACCTGAGTAACGTAGCCATTGCTCGGCGCGCGAAt                                                                       >  1:185049/1‑251 (MQ=255)                                                                                                                                                                      ccGCCTGGCACGACAGGTAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGAAGACCATCACCGGACGCAGCGGCAAGGCAGCTGCGTATGTACCTGGGCGGATCAGTACCTGAGTAACGTAGCCATTGCTCGGCGCGCGAATGAc                                                                    <  1:502939/251‑1 (MQ=255)                                                                                                                                                                       cGCCTGGCACGACAGGTAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACGTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGAGGAAGACCATCACCGGACGCAGCGGCAAGGCAGCTGCGTATGTACCTGGGCGGATCAGTACCTGAGTAACGTAGCCATTGCTCGGCGCGCGAATGACa                                                                   <  1:220329/251‑1 (MQ=255)                                                                                                                                                                           gggCACGACAGGTAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGTCAATTTGCCGTTTTTGCTCGGGGATGAAGACCATCACCGGACGCAGCGGCAAGGCAGCTGCGTATGTACCTGGGTGGATCAGTACCTGAGTAACGTAGCCATTGCTCGTCGCGCGAATGACAGTCt                                                               <  1:59147/250‑1 (MQ=255)                                                                                                                                                                                  aCAGGTAAAATACTAGGCAGTTTGCCGTGAAACACCTGCCCATGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGAAGACCATCACCGGACGCAGCGGCAAGGCAGCTGCGTATGTACCTGGGCGGATCAGTACCTGAGTAACGTAGCCATTGCTCGGCGCGCGAATGACAGTCTGCTCAAg                                                        <  1:80706/251‑1 (MQ=255)                                                                                                                                                                                      gtaatagacgttgcAGTTAGCCGTGAAACTCCGGACCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGGCTCAGACGTACCAGCTAGTTTTGCCGAAATTGGGCGACAATTTGCCTTTTGTGCGCGGGGATGAAGACCATCACCGGACGCATCGGCAAGGCAGCTGCGTATGTACCTGGGCGGATTAGTACCTGAGTAACGTAGCCATTGCTCGGCGTGCGAATGACAGTCTGCTCAAGGTta                                                    <  1:329666/238‑1 (MQ=255)                                                                                                                                                                                        aaaaTACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGAAGACCATCACCGGACGCAGCGGCAAGGCAGCTGCGTATGTACCTGGGCGGATCAGTACCTGAGTAACGTAGCCATTGCTCGGCGCGCGAATGACAGTCTGCTCAAGGTtat                                                   >  1:420963/1‑250 (MQ=255)                                                                                                                                                                                         aaaTACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGGTAAACCTCACTTCCGCATCAACGCCCGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGATTAAGACCATCACCGGACGCAGCGGCAAGTCAGCTGCGTATGTACCTGGGCGGATCAGTACCTGAGTAACGTAGCCATTGCTCGGCGCGCGAATGACAGTCTGCTCAAGGTTatat                                                 <  1:465649/251‑1 (MQ=255)                                                                                                                                                                                             aCTAGACAGTGTGCCGTTAAAACCCTGACCAGGTAGCGCGTTAAACATCCCTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAAGTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGAAGACCATCACCGGACGCAGCGGCAAGGCAGCTGCGTATGTACCTGGGCGGAGCAGTACCTGAGTAACGTAGCCATTGCTCGGCGCGCGAATGACAGTCTGCCCAAGGTTATATTTTg                                             <  2:490210/251‑1 (MQ=255)                                                                                                                                                                                                 gTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGAAGACCATCACCGGACGCAGCGGCAAGGCAGCTGCGTATGTACCTGGGCGGATCAGTACCTGAGTAACGTAGCCATTGCTCGGCGCGCGAATGACAGTCTGCTCAAGGTTATATTTTGCTTc                                         >  1:269081/1‑251 (MQ=255)                                                                                                                                                                                                   caggttaccGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGACAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATAGGGCGACAATTTGCCGTTTTTGCTCGGGGATGAAGACCATCACCGGACACAGCGGCAAGGCAGCTGCGTATGTACCTGGGCGGCTCAGTAACTGAGTAACGTAGCCAATGCTCGGCGCGCGAATGACAGTATGCTCAAAGTTATATTTAGCTTcgg                                       >  2:163254/8‑249 (MQ=255)                                                                                                                                                                                                   cAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGAAGACCATCACCGGACGCAGCGGCAAGGCAGCTGCGTATGTACCTGGGCGGATCAGTACCTGAGTAACGTAGCCATTGCTCGGCGCGCGAATGACAGTCTGCTCAAGGTTATATTTTGCTTCa                                        <  2:200131/250‑1 (MQ=255)                                                                                                                                                                                                    aGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGAAGACCATCACCGGACGCAGCGGCAAGGCAGCTGCGTATGTACCTGGGCGGATCAGTACCTGAGTAACGTAGCCATTGCTCGGCGCGCGAATGACAGTCTGCTCAAGGTTATATTTTGCTTCAGt                                      >  1:484456/1‑251 (MQ=255)                                                                                                                                                                                                      tttGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGAAGACCATCACCGGACGCAGCGGCAAGGCAGCTGCGTATGTACCTGGGCGGATCAGTACCTGAGTAACGTAGCCATTGCTCGGCGCGCGCATGACAGTCTGCTCAAGTGTATATTTTGCTTCAGTa                                     >  2:132838/1‑250 (MQ=255)                                                                                                                                                                                                           cGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGAAGACCATCACCGGACGCAGCGGCAAGGCAGCTGCGTATGTACCTGGGCGGATCAGTACCTGAGTAACGTAGCCATTGCTCGGCGCGCGAATGACAGTCTGCTCAAGGTTATATTTTGCTTCAGTAAGTTgc                               >  1:392265/1‑251 (MQ=255)                                                                                                                                                                                                                  cacCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGAAGACCATCACCGGACGCAGCGGCAAGGCAGCTGCGTATGTACCTGGGCGGATCAGTACCTGAGTAACGTAGCCATTGCTCGGCGCGCGAATGACAGTCTGCTCAAGGTTATATTTTGCTTCAGTAAGTTGCGCTCtt                         >  2:224453/1‑250 (MQ=255)                                                                                                                                                                                                                  cacCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGAAGACCATCACCGGACGCAGCGGCAAGGCAGCTGCGTATGTACCTGGGCGGATCAGTACCTGAGTAACGTAGCCATTGCTCGGCGCGCGAATGACAGTCTGCTCAAGGTTATATTTTGCTTCAGTAAGTTGCGCTCTTa                        <  1:83707/251‑1 (MQ=255)                                                                                                                                                                                                                    ccTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGAAGACCATCACCGGACGCAGCGGCAAGGCAGCTGCGTATGTACCTGGGCGGATCAGTACCTGAGTAACGTAGCCATTGCTCGGCGCGCGAATGACAGTCTGCTCAAGGTTATATTTTGCTTCAGTAAGTTGCGCTCTTAAg                      <  1:326839/251‑1 (MQ=255)                                                                                                                                                                                                                     cTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGAAGACCATCACCGGACGCAGCGGCAAGGCAGCTGCGTATGTACCTGGGCGGATCAGTACCTGAGTAACGTAGCCATTGCTCGGCGCGCGAATGACAGTCTGCTCAAGGTTATATTTTGCTTCAGTAAGTTGCGCTCTTAAGc                     >  1:132120/1‑251 (MQ=255)                                                                                                                                                                                                                                    gTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGCACTTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGAAGACCATCACCGGACGCAGCGGCAAGGCAGCTGCGTATGTACCTGGGCGGATCAGTACCTGAGTCACGTAGCCATTGCTCGGCGCGCGAATGACAGTCTGCTCAAGGCTATATTTTGCTTCAGTAAGTTGCGCTCTTAAGCTCACACTCCGCGAtt      >  2:117213/1‑251 (MQ=255)                                                                                                                                                                                                                                        aaCATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGAAGACCATCACCGGACGCAGCGGCAAGGCAGCTGCGTATGTACCTGGGCGGATCAGTACCTGAGTAACGTAGCCATTGCTCGGCGCGCGAATGACAGTCTGCTCAAGGTTATATTTTGCTTCAGTAAGTTGCGCTCTTAAGCTCACAATCTGCGATTGCTc  >  1:350410/1‑251 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                       ACCGAAACATGGCTGAAATGGTCGGAGTAAACCGCCACCTGGGCGTAGATGCCGTCGGGTAAGGCATCGATATCATCGTTAGGGTCCAGTTCAATGGTTCCCAGCACACCGTCCGTGCCGGGCACGACCGTTAATGATTGCAATACCCCCTGCGCCTGATAAGAACCGCCTGGCACGACAGGTAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACACCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGAAGACCATCACCGGACGCAGCGGCAAGGCAGCTGCGTATGTACCTGGGCGGATCAGTACCTGAGTAACGTAGCCATTGCTCGGCGCGCGAATGACAGTCTGCTCAAGGTTATATTTTGCTTCAGTAAGTTGCGCTCTTAAGCTCACAATCTGCGATTGCTC  >  NC_000913/3770304‑3770785

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑