Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,730,560	C→T	W69* (TGG→TAG)	xylA ←	xylose isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,730,560	0	C	T	100.0%	144.6 / NA	46	W69* (TGG→TAG)	xylA	xylose isomerase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (23/23);  total (23/23)

GCCGTAGCGAGGGTTTGTAAAGCAGTTGGCCGTTCCCCACAGCAGCTTCACGCCGCTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGT‑ACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTG‑CCTCACCAGGCTGCTGCCACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGA‑ATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCATATTGAACTCCATAATCAGGTAATGCCGCGGGTGATGGATG  >  NC_000913/3730313‑3730805                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                       gcCGTAGCGAGGGTTTGTAAAGCAGTTGGCCGTTCCCCACAGCAGCTTCACGCAGCTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCACAATTATTGATGT‑ACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCAAACGCCAGGG‑CCTCACCAGGCTGCTGCTACg                                                                                                                                                                                                                                                     >  2:395235/1‑251 (MQ=255) gcCGAAGCGAGGGTTTGTAAAGCAGTTGGCCGTTCCCCACAGCAGCTTCACGCCGCTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGT‑ACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTG‑CCTCACCAGGCTGCTGCTACg                                                                                                                                                                                                                                                     <  1:928/251‑1 (MQ=255)  ccGTAGCGAGGGTTTGTAAAGCAGTTGGCCGTTCCCCACAGCAGCTTCACGCCGCTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGT‑ACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTG‑CCTCACCAGGCTGCTGCTACgg                                                                                                                                                                                                                                                    >  1:471812/1‑251 (MQ=255)   cGTAGCGAGGGTTTGTAAAGCAGTTGGCCGATCCCCACAGCAGCTTCACGCCGCTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTCTTGATGT‑ACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATAGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCAAACGCCAGTG‑CCTAAACAGGCTGCTGCTACgtc                                                                                                                                                                                                                                                   >  2:15610/1‑249 (MQ=255)              tttGGAAAGCAGTTGGCCGTCCCCCACAGCAGCTTCACGCCGCTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTAGTGATGT‑ACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTG‑CCTCACCAGGCTGCTGCTACGGACGATTAAACg                                                                                                                                                                                                                                         <  2:197330/250‑1 (MQ=255)               ttGTAAAGCAGTTGGCCGTTCCCCACAGCAGCTTCACGCCGCTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGT‑ACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTG‑CCTCACCAGGCTGCTGCTACGGACGATTAAACGc                                                                                                                                                                                                                                        <  2:471812/250‑1 (MQ=255)                  tAAAGCAGTTGGCCGTTCCCCACAGCAGCTTCACGCCGCTCTCTTCTTGCTTGCCTGCCAGGACCTCAACCATTTGCGCAAAATTATTGATGT‑ACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCCCATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTG‑CCTCACCAGGCTGCTGCTACGGACGATTAAACGccccc                                                                                                                                                                                                                                    >  2:479230/1‑251 (MQ=255)                     aGCAGTTGGCCGTTCCCCACAGCAGCTTCACGCCGCTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGT‑ACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTG‑CCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCcaca                                                                                                                                                                                                                                 <  1:90388/251‑1 (MQ=255)                          ttGGCCGTTCCCCACAGCAGCTTCACGCCGCTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAACTTATTGATGT‑ACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTG‑CCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCaa                                                                                                                                                                                                                             >  2:391680/1‑250 (MQ=255)                          ttGGCCGTTCCCCACAGCAGCTTCACGCCGCTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGT‑ACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTG‑CCTCACCTGGCTGCTGCTACGGACGATTAAACGCCCCCACACCaa                                                                                                                                                                                                                             >  1:43053/1‑250 (MQ=255)                                  tCCCCACAGCAGCTTCACGCCGCTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGT‑ACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGGG‑CCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCAACACCAAACATATCCg                                                                                                                                                                                                                    >  2:412517/1‑251 (MQ=255)                                    cccACAGCAGCTTCACGCCGCTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGT‑ACTCTTTTAACGACGCGCCCTCCGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTG‑ACTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGcc                                                                                                                                                                                                                  >  2:15531/1‑251 (MQ=255)                                      cacggcagcTGCACGCGTCTCTCTTTTTGCTTGCCTGCCAGGACATCAACTATTTGCGCAAAATTATTGATGT‑ACTCGTTTAACGACGCGCCCTCAGGGGAAACAGCCACAGCGTGGAAGTAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTCCGCCAACGCCAGTG‑CCTCACCAGGCTGGTTCTACGGTCGATTAATCGCCCCCACATCAAACATATCCGcccc                                                                                                                                                                                                                <  1:446570/247‑1 (MQ=255)                                      cacaGCAGCTTCACGCCGCTCTCTTCTTGCTTGCCTGCCAGGACATCCACCATTTGCGCAAAATTATTGATGT‑ACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTG‑CCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGcccc                                                                                                                                                                                                                >  2:423592/1‑251 (MQ=255)                                           ccgtttCACTCCGCTCTCTTCTTGCTTGCCTTCCAGGCCATCAACCATTTGCGCAAAATTATGTATTG‑ACTCGTTTAACGACGCGCCCTCAGGGGAAACACCCTTATCTTGTAAGCAATAAAATGGCACATGTTACTTTTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCACCGCCAGTG‑CCTCACCATGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTcc                                                                                                                                                                                                           <  2:400240/247‑1 (MQ=255)                                                              tcttGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGT‑ACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTG‑CCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCa                                                                                                                                                                                           <  1:475437/248‑1 (MQ=255)                                                                 tgcttgcCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGT‑ACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTG‑CCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAg                                                                                                                                                                                       <  1:138775/249‑1 (MQ=255)                                                                  tcttgacttccaggaacgcAACCATTTGCGCAAAATTATTGATGT‑AATCGTTTAACGACGCGTCCTCAGGGTAAACATCCACATCTTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACGCAAATGCGACATCGGCTTTACGCTGCGCCAACTCCAGTG‑CCTCACCTGGCTGCTGTTACGGACTATTTAACGCCCTCTCACCAAGCATATCCGCCCCGTTCCATAAGAAGGTGTGCCAGTGGCa                                                                                                                                                                                     <  2:496514/232‑1 (MQ=255)                                                                  gcttgcCTGCCAGGACATCAACCATTTGCGCACAATTATTGATGT‑ACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAAATCAAATGCGACATCTGCTTTACGCTTAGCCAACGCCAGTG‑CCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGc                                                                                                                                                                                      >  2:95656/1‑249 (MQ=255)                                                                    gggccttccAGGACACAAACCATTAGCGCAAAATTATTGATGTCACT‑TTTTAACTACGCTCCCTCAGGGGAAACATCCACATCGAGGAAGCAATAAAATTGCACATGTATCTTGTGGAAAAACTCAAATGCTACATCCGCTGTACGCTTCGCCAACGCCAGTG‑CCTCACCAGGCTTCTGCTTCGGACGATTAAACGCCCCCACACCAAACAGAGCCGCCCCGGTCCAGCAGAATGTGGGCCAGTAGCAggc                                                                                                                                                                                  <  2:283511/249‑1 (MQ=255)                                                                       cctgccAGGACATCAACCATTTGCGCAAAATTATTGATGT‑ACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCCCATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTG‑CCTCACCAGGCTGCTGCTACGGACGATTAAACGCCACCACACCAAACATATCCGCCCCGTTCCAGAAGAAGGTGTGCCAGTAGCAggcggc                                                                                                                                                                               >  1:34785/1‑251 (MQ=255)                                                                           ccAGGACATCAACCATTTGCGCAAAATTATTGATGT‑ACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTG‑CCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCaaa                                                                                                                                                                            >  1:317931/1‑250 (MQ=255)                                                                                    cAACCCTTTGCGAAAAATTATTGATGT‑ACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCGACGCCAGTG‑CCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTCGCAGGCGGAAAAACGCAAgggc                                                                                                                                                                  >  2:398280/1‑248 (MQ=255)                                                                                             gagcAAAATTATTGATGT‑ACACTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGGGGAAGCAATAAAATGGCACAGTTAACTTGTGGAAAAACCCAAATGCGACAGCTGCTTTACGCTTCGCCAACGCGAGTG‑CCTCACCAGGCTGCTGCTACGGACGATTAAGCGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAACCGCAAGTTCTCTGCCATa                                                                                                                                                         <  2:456203/249‑1 (MQ=255)                                                                                                 aaaaTTATTGATGT‑ACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTG‑CCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCt                                                                                                                                                     >  1:130772/1‑251 (MQ=255)                                                                                                       attGATGT‑ACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTG‑CCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTAccc                                                                                                                                                <  1:398280/250‑1 (MQ=255)                                                                                                                                   cgcAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCGTCGCCAACGCCAGTG‑CCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCGTACCCAACACCAGTTCGTCGGGATTGTAGTGAc                                                                                                                    <  2:179286/249‑1 (MQ=255)                                                                                                                                        gggAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTG‑CCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGA‑At                                                                                                              <  2:154300/251‑1 (MQ=255)                                                                                                                                                        gTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTG‑CCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACAACAGTTCGTCGGGATTGTAGTGACGGA‑ATGCTAACGGGTTTGAgg                                                                                              >  2:32717/1‑251 (MQ=255)                                                                                                                                                          ggAATCAATAAAATGGCACATGTAACTTGTGGAAAAACTCACATGCGACATCTGCTTTACGCTTCGCCACCGCCAGTG‑CCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTGCCATACGCTTACCCAACACCAGTTCGTCGGAATTGTAGTGACGGA‑ATGCTAACGGGTTggagga                                                                                             <  2:97598/250‑1 (MQ=255)                                                                                                                                                                     aaTGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTG‑CCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATAAGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGA‑ATGCTAACGGGTTTGAGGATTTTGAGCCTTc                                                                                 >  2:431339/1‑251 (MQ=255)                                                                                                                                                                         gCACATGTAACTGGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTG‑CCTCTCCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATAACCGCCCCGTTCCAGCAGAAGGTGTTCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGA‑ATGCTAACGGGTTTGAGGATTTTGAGCCTTCATaa                                                                             <  2:41473/251‑1 (MQ=255)                                                                                                                                                                              tGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTG‑CCTCACCAGGCTGCTGCTACGGACGATTAACCGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGCGCCAGTAGCATGCGGCAAATCGCAAGTGCTCTTCAATAAGCTTACCCAACACCAGTTCGTCGGGATTGTCGTAACGGA‑TTGATACCGGGTATGTGGCTTTTGAGCCTTCCTaacgcac                                                                        >  2:135397/1‑248 (MQ=255)                                                                                                                                                                              tGTAAATTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTG‑CCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGA‑ATGCTAACGGGTTTGAGGATTTTGAGCCTTCATaacgaac                                                                        >  2:30522/1‑251 (MQ=255)                                                                                                                                                                               gTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTG‑CCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGA‑ATGCTAACGGGTTTGAGGATTTTGAGCCTTCATaacgaac                                                                        <  1:188492/250‑1 (MQ=255)                                                                                                                                                                               gTAACTTGTGGAAAAACTCAAATGCGACACCTTCTTTACGCTTCGCCAACGCCA‑TGTCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCTTCGGGATTGTAGTGACGGA‑ATGCTAACGGGTTTGAGGATTTTGAGCCTTCACaacgaac                                                                        <  2:36993/250‑1 (MQ=255)                                                                                                                                                                                          aaaaaCTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTG‑CCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGA‑ATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTg                                                            >  1:96666/1‑251 (MQ=255)                                                                                                                                                                                                     agCGACATCTGCTTTACGCTTCGCCAACGCCAGTG‑CCTTACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGA‑ATGCTAACGGGTTTGAGGATTTTGAGCCGTCATATCTAACGCGAGCGAGCTGGTCAAAATAgg                                                 <  1:121189/250‑1 (MQ=255)                                                                                                                                                                                                       cGACATCTGCTTTACGCTTCGCCAACGCCAGTG‑CCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGA‑ATGCTAACGGGTTTGAGGATTTTGAGCCTTCATaacgaacg                                                                       <  2:246909/227‑1 (MQ=255)                                                                                                                                                                                                       cGACATCTGCTTTACGCTTCGCCAACGCCAGTG‑CCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGA‑ATGCTAACGGGTTTGAGGATTTTGAGCCTTCATaacgaacg                                                                       >  1:246909/1‑227 (MQ=255)                                                                                                                                                                                                         aactctgcttttacctacGCCAACCCCATTT‑CCTCCCCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGA‑ATGCTAACGGGTTTGAGGATTTTTAGCCTTCATAACTAACGCGATCGAGCTGGTCAAAATAGGc                                                <  2:463857/231‑1 (MQ=255)                                                                                                                                                                                                                  ttACGCTTCGCCAACGCCAGTG‑CCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGA‑ATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCATATTGa                                     >  1:327338/1‑250 (MQ=255)                                                                                                                                                                                                                          cGCCAACGCCAGTG‑CCTCACCAGGATGCTGCTACGGACGATTAAACGCCCCCACACCAACCATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTCGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGACGGGATTGTAGTGACGGATAT‑CTAACGGGGTTGAGGGTTTTGAGACTTCATAACGAGAGCCATCAAGCTGGTCaaaaatgtcgtgtatatttaactcctaa                             >  2:46636/1‑226 (MQ=255)                                                                                                                                                                                                                                 gCCAGTG‑CCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGA‑ATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCATATTGAACTCCATAATCAGGt                      >  1:134854/1‑250 (MQ=255)                                                                                                                                                                                                                                         ccTCTCCAGGCTGCGGGTACGGACGATTAAACGCCCCATCACCAAACAAATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGTCAAAACGCAAGTGCTCTTCCATACGCTTTCCCAACACCAGTGCGTCGGGATTGTAGTGACGGA‑ATGCTAACGGTTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCATATTGAACTCCATAATCAGGTAAGGCcgc              <  2:168128/251‑1 (MQ=255)                                                                                                                                                                                                                                               cAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGA‑ATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCATATTGAACTCCATAATCAGGTAATGCCGCGGGTga        <  1:339329/251‑1 (MQ=255)                                                                                                                                                                                                                                                     gctgctACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGA‑ATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCATTTTGAACCCCATAATCAGGTAATGCCCCGGGTgatggttg  >  2:13604/1‑248 (MQ=255)                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                       GCCGTAGCGAGGGTTTGTAAAGCAGTTGGCCGTTCCCCACAGCAGCTTCACGCCGCTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGT‑ACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTG‑CCTCACCAGGCTGCTGCCACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGA‑ATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCATATTGAACTCCATAATCAGGTAATGCCGCGGGTGATGGATG  >  NC_000913/3730313‑3730805

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑