Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,433,900	C→T	L71L (CTA→TTA)	def →	peptide deformylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,433,900	0	C	T	95.3%	115.4 / ‑1.9	44	L71L (CTA→TTA)	def	peptide deformylase
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base T (24/17);  total (26/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.79e-01

ACTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGCTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAA‑ACAGGCATTGAAGAAGGTTGCCTGTCGATCCCTGAACAACGTGCTTTAGTGCCGCGCGCAGAGAAAGTTAAAATTCGCGCCCTTGACCGCGACGGTAAACCATTTGAACTGGAAGCAGACGGTCTGTTAGCCATCTGTATTCAGCATGAGATGGATCACCTGGTCGGCAAACTGTTTATGGATTATCTGTCACCGCTGAAAC  >  NC_000913/3433664‑3434143                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                     attCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGATaatcaat                                                                                                                                                                                                                                        >  1:299340/3‑251 (MQ=255)       gAACCCATCTCTGGAGATTTATGGCAGTTTTGCAAGTGTTACATATTCCGGACTAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCagag                                                                                                                                                                                                                                  <  2:499914/251‑1 (MQ=255)       gAACACATCTCTGGAGATTTAGGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCaga                                                                                                                                                                                                                                   <  2:382699/250‑1 (MQ=255)               ctctGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGACCGGTTAGTGTTAATCAATCCAGAGCtttt                                                                                                                                                                                                                             >  2:129936/1‑248 (MQ=255)                 ctGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGCTATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGaa                                                                                                                                                                                                                         >  2:353902/1‑250 (MQ=255)                         ttATGGCAGTTTTGCAAGAGTTACATATGCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTGTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAgcgg                                                                                                                                                                                                                 <  2:190921/250‑1 (MQ=255)                                      gCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAA‑ACAGGCATTg                                                                                                                                                                                                  >  1:173582/1‑251 (MQ=255)                                         aGTGTGACATTTGCCGGACGAACGGCTTCGAACATTTGCTAAACCGGTAGACGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAAAAGCTGTACGCAGAAGAGGGTATTGACCTGGCGGCCACCCCGGTTGATTTCCTTCCACGTCTCATTGTTATTGTTGCTTCGGGAAACCGTGCAGAACGTATTGTGATAATCCATCCAACGCTTTTAGAAAAAAacggcaacacagacaatga                                                                                                                                                                                                  >  2:81973/1‑235 (MQ=255)                                                cATATTCCGGACGAGCGGCTTCGCAAAGATGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGACGATGATATGTTCAAGACGATGCACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGCTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGCGGTAATCAATCCAGAGCTTTTCAACAAAAGAGGAGAA‑ACAGGCATTGAAACAGGTTg                                                                                                                                                                                        >  2:378908/1‑251 (MQ=255)                                                      ccGGACGAGCGGCTTCGCAAAGGTGCTAAACCGGTAGAAGAATTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGAATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACCAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGCAAAAAGCGGCCAA‑ACCAGCATTAAAGAAGGTTGCCTGTc                                                                                                                                                                                  >  2:12254/1‑251 (MQ=255)                                                       cGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTaat                                                                                                                                                                                                                                            >  1:185090/1‑193 (MQ=255)                                                       cGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTaat                                                                                                                                                                                                                                            <  2:185090/193‑1 (MQ=255)                                                             aGCGGCTTCGCAAAGTTGCTAAACCGTCATAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAAGCCAGAGCTTTTAGAAAAAAGCGGCGAA‑ACAGGCATTGAAGAAGGTTGCCTTTCGATCCCt                                                                                                                                                                           <  2:334517/251‑1 (MQ=255)                                                                            ttGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCCGCGTATCGTCGATGATATGTTCGAGTCGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGATGATATCCATCAACGTCTCATTGTTATTGATGTTTCGGACCACCGTGACGAACGGTTAGTGTTACTCAATCCAGCGCTTTTAGAAAAACAAGTCAAA‑ACAGACATTGAAGAAGGTTGCATGTCGATCACTGACCAACGTGCtttt                                                                                                                                                            >  2:473344/1‑250 (MQ=255)                                                                              gCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAA‑ACAGGCATTGAAGAAGGTTGCCTGTCGATCCCTGAACAACGTGCTTTAGt                                                                                                                                                          <  1:129936/251‑1 (MQ=255)                                                                              gCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGGATTGGCCTGGCGGCAACACAGGTTGATATCCAACAACGTATCATTGGTATTGATGTTTCGGAAATCCGGGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTGAGAAAAAAGCGGCGAA‑ACAGGCATTGAAGAAGGTTGCCTGTCGATCCCTGAACAACGTGCTTTAGt                                                                                                                                                          <  2:441365/251‑1 (MQ=255)                                                                                 aaaCCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAA‑ACAGGCATTGAAGAAGGTTGCCTGTCGATCCCTGAACAACGTGCTTTAGTg                                                                                                                                                         <  1:353902/249‑1 (MQ=255)                                                                                    ccGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAA‑ACAGTCATTGAAGAAGGTTGCCTGTCGATCCCTGAACAACGTGCTTTAGTGCcgcg                                                                                                                                                    <  1:51329/251‑1 (MQ=255)                                                                                     cGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAA‑ACAGGCATTGAAGAAGGTTGCCTGTCGATCCCTGAACAACGTGCTTTAGTGCcgcgc                                                                                                                                                   >  1:149225/1‑251 (MQ=255)                                                                                      ggtggaagaaGTGAATGCAGAAATTCAGCGTATCGTCGATGCTATGTTCCAGACGATGTACGCAGAAGAAGGAATTGGCGTGGCGGCAAACCAGGTTGATATCCATCGAAGTATCATTGTTATTGATGTTTCGGAAAACCGAGCCGAAAGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGAGGAGAATAC‑GGCATTGAAGAAGGTAGCCTTTCGATCCCTGAAAAACGTACTTAAGGGCcgcgcg                                                                                                                                                  >  2:326505/5‑251 (MQ=255)                                                                                          gaagaaGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAA‑ACAGGCATTGAAGAAGGTTGCCTGTCGATCCCTGAACAACGTGCTTTAGTGCCgcgcgc                                                                                                                                                 >  1:419719/1‑248 (MQ=255)                                                                                          gaagaaGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAA‑ACAGGCATTGAAGAAGGTTGCCTGTCGATCCCTGAACAACGTGCTTTAGTGCCgcgcgc                                                                                                                                                 >  1:28735/1‑248 (MQ=255)                                                                                             gaagTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAA‑ACAGGCATTGAAGAAGGTTGCCTGTCGATCCCTGAACAACGTGCTTTAGTGCCGCGCGCAGAGaa                                                                                                                                           <  1:286403/251‑1 (MQ=255)                                                                                                  gAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGAGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCAAGAGCTTTTAGAACAAAGCGGCGAA‑ACAGGCATTGAAGACGGTTGCCTGTCGATCCCTGAACAACGAGCTTTAGTGCCGCGCGCAGAGAAAGtt                                                                                                                                       >  2:251025/1‑250 (MQ=255)                                                                                                             ttCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAA‑ACAGGCATTGAAGAAGGTTGCCTGTCGATCCCTGAACAACGTGCTTTAGTGCCGCGCGCAGAGAAAGTTAAAATTCGCGcc                                                                                                                           >  2:313388/1‑251 (MQ=255)                                                                                                                     aTCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAA‑ACAGGCATTGAAGAAGGTTGCCTGTCGATCCCTGAACAACGTGCTTTAGTGCCGCGCGCAGAGAAAGTTAAAATTCGCGCCCTTGACcg                                                                                                                   >  1:225092/1‑251 (MQ=255)                                                                                                                            atgatATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAA‑ACAGGCATTGAAGAAGGTTGCCTGTCGATCCCTGAACAACGTGCTTTAGTGCCGCGCGCAGAGAAAGTTAAAATTCGCGCCCTTGACCGCGACgg                                                                                                             <  1:112718/250‑1 (MQ=255)                                                                                                                                        agaCGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGTCGAACGGCTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAA‑ACAGGCATTGAAGAAGGTTGCCTGTCGATCCCTGAACAACGTGCTTTAGTGCCGCGCGCAGAGAAAGTTAAAATTCGCGCCCTTGACCGCGACGGTAAAACATTTGa                                                                                                 >  1:134617/1‑250 (MQ=255)                                                                                                                                        agaCGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAA‑ACAGGCATTGAAGAAGGTTGCCTGTCGATCCCTGAACAACGTGCTTTAGTGCCGCGCGCAGAGAAAGTTAAAATTCGCGCCCTTGACCGCGACGGTAAACAATTTGa                                                                                                 >  1:187346/1‑250 (MQ=255)                                                                                                                                              ggTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAA‑ACAGGCATTGAAGAAGGTTGCCTGTCGATCCCTGAACAACGTGCTTTAGTGCCGCGCGCAGAGAAAGTTAAAATTCGCGCCCTTGACCGCGACGGTAAACCATTTGAACTGGaa                                                                                          <  1:194631/250‑1 (MQ=255)                                                                                                                                               gTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAA‑ACAGGCATTGAAGAAGGTTGCCTGTCGATCCCTGAACAACGTGCTTTAGTGCCGCGCGCAGAGAAAGTTAAAATTCGCGCCCTTGACCGCGACGGTAAACCATTTGAACTGGa                                                                                           >  1:452031/1‑249 (MQ=255)                                                                                                                                                    cAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAA‑ACAGGCATTGAAGAAGGTTGCCTGTCGATCCCTGAACAACGTGCTTTAGTGCCGCGCGCAGAGAAAGTTAAAATTCGCGCCCTTGACCGCGACGGTAAACCATTTGAACTGGAAGCAGAc                                                                                    >  2:189654/1‑251 (MQ=255)                                                                                                                                                               aTTGGCCGGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTCGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAA‑ACAGGCATTGAAGAAGGTTGCATGTCGCTCCCTGAACATCGTGCTTTAGTGCCGCGCGCAGAGAAAGTTAAACTTAGCGCACTTGACCGCGACGATAAACCCTTTGACCTGGAAGAAGCCGACCTGTTAGc                                                                         >  2:124954/1‑251 (MQ=255)                                                                                                                                                                    ccTGGTGGCAACCCAGGTTTATATCCATCAACGTATCATTGTTATTGATGTTGCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAA‑ACAGGCATTGAAGAAGGTTGCCTGTCGATCCCTGAACAACGTGCTTTAGTGCCGCGCGCAGAGAAAGTTAAAATTCGCGCCCTTGACCGCGACGGTAAACCAATTGAACTGGAAGCAGACGGTCTGTTAGCCATCt                                                                    <  2:452031/251‑1 (MQ=255)                                                                                                                                                                       ggcggcAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTGAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAA‑ACAGGCATTGAAGAAGGTTGCCTGTCGATCCCTGAACAACGTGCTTTAGTGCCGCGCGCAGAGAAAGTTAAAATTCGCGCCCTTGACCGCGACGGTAAACCATTTGAACTGGAAGCAGACGGTCTGTTAGCCATCTg                                                                   <  2:28735/249‑1 (MQ=255)                                                                                                                                                                                ccAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAA‑ACAGGCATTGAAGAAGGTTGCCTGTCGATCCCTGAACAACGTGCTTTAGTGCCGCGCGCAGAGAAAGTTAAAATTCGCGCCCTTGACCGCGACGGTAAACCATTTGAACTGGAAGCAGACGGTCTGTTAGCCATCTGTATTCAGCATg                                                        >  1:276552/1‑251 (MQ=255)                                                                                                                                                                                   ggTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAA‑ACAGGCATTGAAGAAGGTTGCCTGTCGATCCCTGAACAACGTGCTTTAGTGCCGCGCGCAGAGAAAGTTAAAATTCGCGCCCTTGACCGCGACGGTAATCCATTTGAACTGGAAGCAGACGGTCTGTTAGCCATCTGTATTCAGCATgaga                                                     <  2:147121/251‑1 (MQ=255)                                                                                                                                                                                    gTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAA‑ACAGGCATTGAAGAAGGTTGCCTGTCGATCCCTGAACAACGTGCTTTAGTGCCGCGCGCAGAGAAAGTTAAAATTCGCGCCCTTGACCGCGACGGTAAACCATTTGAACTGGAAGCAGACGGTCTGTTAGCCATCTGTATTCAGCATGAGAt                                                    <  2:142960/251‑1 (MQ=255)                                                                                                                                                                                                      aTCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAA‑ACAGGCATTGAAGAAGGTTGCCTGTCGATCCCTGAACAACGTGCTTTAGTGCCGCGCGCAGAGAAAGTTAAAATTCGCGCCCTTGACCGCGACGGTAAACCATTTGAACTGGAAGCAGACGGTCTGTTAGCCATCTGTATTCAGCATGAGATGGATCACCTGGTCGGCa                                   >  1:482575/1‑250 (MQ=255)                                                                                                                                                                                                       tCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAA‑ACAGGCATTGAAGAAGGTTGCCTGTCGATCCCTGAACAACGTGCTTTAGTGCCGCGCGCAGAGAAAGTTAAAATTCGCGCCCTTGACCGCGACGGTAAACCATTTGAACTGGAAGCAGACGGTCTGTTAGCCATCTGTATTCAGCATGAGATGGATCACCTGGTCGGCaa                                  >  1:352091/1‑250 (MQ=255)                                                                                                                                                                                                                             aaaCCGGGTCGAACGGCTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAA‑ACAGGCATTGAAGAAGGTTGCCTGTCGATCCCTGAACAACGTGCTTTAGTGCCGCTCGCAGAGAAAGTTAAAATTCGCGCCCTTGACCGCGACGGTAATCCATTTGAACTGGAAGCAGACGGTCTGTTAGCCATCTGTATTCAGCATGAGATGGATCACCTGGTCGGCAAACTGTTTATGGATTATCTGTCAc           <  2:134617/251‑1 (MQ=255)                                                                                                                                                                                                                              aaCCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAA‑ACAGGCATTGAAGAAGGTTGCCTGTCGATCCCTGAACAACGTGCTTTAGTGCCGCGCGCAGAGAAAGTTAAAATTCGCGCCCTTGACCGCGACGGTAAACCATTTGAACTGGAAGCAGACGGTCTGTTAGCCATCTGTATTCAGCATGAGATGGATCACCTGGTCGGCAAACTGTTTATGGATTATCTGTCAcc          <  1:313388/251‑1 (MQ=255)                                                                                                                                                                                                                                  gTGACGCACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAA‑ACAGGCATTGAAGAAGGTTGCCTGTCGATCCCTGAACAACGTGCTTTAGTGCCGCGCGCAGAGAAAGTTAAAATTCGCGCCCTTGACCGCGCCGGTAACCCATTTGAACTGGAAGCAGACGGTCTGTTCGCCATCTGTATTCAGCATGAGATGGATCACCTGGCCGGCAAACTGATTATGGATTATCTGTCACCGCTg      >  2:359451/1‑251 (MQ=255)                                                                                                                                                                                                                                       gAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGAGAA‑ACAGGCATTGAAGAAGGTTGCCTGTCGATCAATGAACAACGTGCTTTAGTGCCGCGCGCAGAGAAAGTTAAAATTCGCGCCCTTGACCGCGACGGTAAACCATTAGAACTGGAAGCAGAAGGTCTGTTAGCCATCTGTATTCAGCATGAGATGGATCACCTGGTCGGCACACTGTTTATGGATTATCTGTCACCGCTGaacc  >  1:257052/1‑248 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                     ACTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGCTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAA‑ACAGGCATTGAAGAAGGTTGCCTGTCGATCCCTGAACAACGTGCTTTAGTGCCGCGCGCAGAGAAAGTTAAAATTCGCGCCCTTGACCGCGACGGTAAACCATTTGAACTGGAAGCAGACGGTCTGTTAGCCATCTGTATTCAGCATGAGATGGATCACCTGGTCGGCAAACTGTTTATGGATTATCTGTCACCGCTGAAAC  >  NC_000913/3433664‑3434143

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑