Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,256,301	G→A	S117S (TCC→TCT)	cyuA ←	putative L‑cysteine desulfidase CyuA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,256,301	0	G	A	100.0%	171.5 / NA	57	S117S (TCC→TCT)	cyuA	putative L‑cysteine desulfidase CyuA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (34/23);  total (34/23)

GCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGTGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACGGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAG  >  NC_000913/3256056‑3256533                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                         gcAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGTGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACAGAGAc                                                                                                                                                                                                                                     >  1:204574/1‑251 (MQ=255)  cAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCAACGTCAGCGGAGACTCTTGATCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGTGAGAAGAGGATTTCATCGAAAGGTTCCTGGATCTTAACAGAGACt                                                                                                                                                                                                                                    >  1:500953/1‑251 (MQ=255)   aaaCGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGACCACTTCTCACCATTCCAGACTTTGGCGCGTGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACAGAGACtt                                                                                                                                                                                                                                   >  1:434228/1‑251 (MQ=255)           cTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGTGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACAGAGACTTTCCccgcc                                                                                                                                                                                                                           >  1:449408/1‑251 (MQ=255)               atagacgacCTCCAGGATCCCCGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACCCCACACCATCGTGCGTCTCGATATGCACAATTTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGTGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACAGAGACTTTCCCCGCCGCca                                                                                                                                                                                                                       <  1:271461/248‑1 (MQ=255)                  gACGAACTTCAGGATCTCAGCCAGCGTCTTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGTGAGACGAGGATTTCATCGCAAGGTTCCTGGATCTTAACAGAGACTTTCCCCGCCGCcagcc                                                                                                                                                                                                                    >  2:143224/1‑250 (MQ=255)                     gAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTATCACCGTTCCAGACTTTGGCGCGTGAGAAGAGGATTTCATCGCAAGTTTCCTGGATCTTAACAGAGACTTTCCCCGCCGCcagcaggg                                                                                                                                                                                                                 >  2:102414/1‑249 (MQ=255)                          tCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGTGCGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACAGAGACTTTCCCCGCCGCCAGCAGTGCTTTg                                                                                                                                                                                                            >  2:480728/1‑251 (MQ=255)                                       cagcGCCGTTCTGTAAAGCCCCGTCAGCGGAGCCTCTTGCTCGCCCTCTCCCACACACGCCTGCTGGGTAAACACCCCACCATCGTGCGCCTCGATATGCACAATGTGGGTATGCCCGCCGACGATGGTAACACGCGCCCACTTCTCGCCGTTCCAGACTTTGGCGCGTGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACAGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTg                                                                                                                                                                                               <  1:15446/251‑1 (MQ=255)                                                    gAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGTGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACAGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGc                                                                                                                                                                                  >  1:315851/1‑251 (MQ=255)                                                       acacccggcAGCGGAGACTCTGTCTCGCCCTCTGCCACACACCCCGGCGGGGTAAACCCCACCCAACCGGGCGTCTCGGTATGCAAACTGTGGGAAGGCCCGCTGACGATGGTGACACACGCCCACTCCCCCCCGTTCCAGACTTGGGCGCGTGAGAAGAGGATTTCAGCGCAAGGTTCCTGGATCTTAACAGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTc                                                                                                                                                                               <  2:209414/243‑1 (MQ=255)                                                        gCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGCCTTTGGCGCGTGAGACGAGGATTTCATCGCAAGGTTCCTGGCTCTTAACAGAGCCTTTCCCCGCCGCCCGCAGTGCTTTGGCGTCGGCAATTGCCTCCGCTGTTGCGTCt                                                                                                                                                                              >  2:341700/1‑251 (MQ=255)                                                         cACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGTGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACAGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCtt                                                                                                                                                                             >  1:46570/1‑251 (MQ=255)                                                           ccGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGTGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACAGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTc                                                                                                                                                                           >  1:339749/1‑251 (MQ=255)                                                              ccAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACCCCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGTGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACAGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGc                                                                                                                                                                        <  1:465717/250‑1 (MQ=255)                                                                aGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGTGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACAGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCCTCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCAc                                                                                                                                                                      >  2:429716/1‑251 (MQ=255)                                                                       aCTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGTGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACAGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGc                                                                                                                                                               >  1:368606/1‑251 (MQ=255)                                                                        ctctTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGTGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTACCAGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTCCCTCCGCTGTTGCGTCTTTCAGCACTTCCAGcc                                                                                                                                                              >  2:29119/1‑251 (MQ=255)                                                                         tctTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGTGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACAGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGccc                                                                                                                                                             >  1:274150/1‑251 (MQ=255)                                                                          ctTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGCTGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGTGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACAGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCg                                                                                                                                                            >  1:331148/1‑251 (MQ=255)                                                                             gCTCGCCCTCTGCCACCCACGCCTGCTGGGTAACCCCCCCCCCACCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGTGAGAAGAGGATTTCACCGCAAGGTTCCTGGATCTTAACAGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGc                                                                                                                                                          <  1:224169/250‑1 (MQ=255)                                                                                 gCCCTCTGCCACACACGCCTGCTGGGTACACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGTGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACAGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTgg                                                                                                                                                     >  2:490894/1‑251 (MQ=255)                                                                                  cccTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGTGAGCAGAGGATTTCATCGCCAGGTTCCTGGATCTTAACAGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCCCTTCCAGCCCGGCGTTggg                                                                                                                                                    >  2:18899/1‑250 (MQ=255)                                                                                  cccTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGTGAGAAGAGGATTTCATCGCCAGGTTCCTGGATCTTAACAGAGACTTTCCCCGCCGCCAGCAATGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTATTTCAGCACTTCCAGCCCGGCGTTGGc                                                                                                                                                    >  1:303436/1‑251 (MQ=255)                                                                                        gCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGTGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACAGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTcc                                                                                                                                              >  2:356851/1‑251 (MQ=255)                                                                                          cacacaCGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATACACAATGTTGGTATGCCCGCCGACGTTGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGTGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACAGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTGGGCATTTCCAc                                                                                                                                            >  1:436267/1‑251 (MQ=255)                                                                                             ccccGCCTGCTGGTTAAACCCCACCCCAGCGGGCGTCTCGATATGCACAATGTTGGTAGGCCCGCCGACGTTGGTGACACTCTCCCACTTCTCCCCGTCCCAGCCTTTGGCGCGTGATAAGAGGATTCCATCGCAAGGTCCCTGTATCTTAACAGAGTCTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTa                                                                                                                                         <  1:247465/248‑1 (MQ=255)                                                                                                 cccTGCTGGGTAAACCCCACACCAGCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGTGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACAGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGc                                                                                                                                     <  1:99269/250‑1 (MQ=255)                                                                                                     gcggggtaacacccacaccaCCGGGCGTCGCGATATGCAAAATGTGGGTATGCCCGCCGACGATGGGGACCCACGCCCACTTCTCCCCGTTCCAGACTTTGGCGCGTGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACAGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGccccc                                                                                                                                 <  2:274150/239‑1 (MQ=255)                                                                                                        gggTAAACACCCCACCACCGGGCGTTTCAATAGCCACAAGGTTGTTATCCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGTGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACAGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAgc                                                                                                                              <  1:18899/251‑1 (MQ=255)                                                                                                                     cccatcgtgcgcccggaatggcaaaaTGTGGGTAGGCCCGCCGACGATGGTGACCCCCGCCCACTTCTCCCCGTTCCAGACTTTGGCGCGTGAGAAGAGGATTTCAGCGCAAGGTCCCTGGATTTTAACATAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCg                                                                                                                 <  1:86339/227‑1 (MQ=255)                                                                                                                     accaTCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGATCCAGACTTTGGCGCGTGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACAGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCg                                                                                                                 >  1:233135/1‑251 (MQ=255)                                                                                                                                 ctcGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGTGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACAGAGACTTTccc                                                                                                                                                                                                                               <  1:261717/129‑1 (MQ=255)                                                                                                                                 ctcGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGTGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACAGAGACTTTccc                                                                                                                                                                                                                               >  2:261717/1‑129 (MQ=255)                                                                                                                                 ctcGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGTGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACAGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCCCTTCCAGCCCGGCGTTGGCATTTCCACCTACCGCCCCCAGCGCCGCCGCAATCGGCAGccccccc                                                                                                      >  2:248419/1‑247 (MQ=255)                                                                                                                                 ctcGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGTGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACAGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGc                                                                                                                                                               <  1:241693/193‑1 (MQ=255)                                                                                                                                   caattggCACAATGTGGTTACGTCCGCCGCCGATGGGGACACACGACCCCTTCTCACCGTTCCAGACTTTGGCGCGTGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTACAAGAGACTTTCCCCGCCGCCAGCAGTGCTTGGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCAtt                                                                                                   <  1:78151/245‑1 (MQ=255)                                                                                                                                       atGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGTGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACAGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCCGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGCCAGCCCCCCCATTcccc                                                                                               >  2:45728/1‑250 (MQ=255)                                                                                                                                                gTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGTGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACAGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGa                                                                                       >  1:413299/1‑250 (MQ=255)                                                                                                                                                     tATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGTGAGAAGAGGATTTCATCGCAAGGTTCCTGGCTCTTAACAGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCAGCAGCCCCACCAGTCCCGAGCCGGGAACGGTg                                                                                 >  2:412854/1‑251 (MQ=255)                                                                                                                                                        ccccGCCGAGGATGGGGACACCCGCCCACTTCCCCCCTTTCCAGACTTTGGCCCGTAAGAATAGGTTTTCATCGCAAGGTTCCTGGATCTTAACAGAGATTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGAc                                                                               <  1:269683/249‑1 (MQ=255)                                                                                                                                                          ccgccgACGATGGTGACACACGCCCACTTCTCACCTTTCCAGACTTTGGCGCGTGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACAGAGACTTCCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGcc                                                                            <  1:237862/251‑1 (MQ=255)                                                                                                                                                           cgccgACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGTGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACAGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGccc                                                                           >  1:331068/1‑251 (MQ=255)                                                                                                                                                            gccgACGATGGTGACACACGCCCACTTCTCCCCGTTCCAGACTTTGGCGCGTGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACAGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCa                                                                          <  1:144273/251‑1 (MQ=255)                                                                                                                                                               gccgaTGGTGACACACGCCCCCTTCTCACCGTTCCAGACTTTGGCGCGTGAGAAGAGGCTTTCATCGCAAGGTTCCTGGATCTTAACAGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGTCGTTGGCCTTTCCACCTAACGCCCCCAGCGCCTCCGCACTCTGCATCCCCACCATTCCCGTGCCGGGCACGGTCACGCCCAGAc                                                                       >  2:201507/3‑251 (MQ=255)                                                                                                                                                                   aTGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGTGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACAGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGtt                                                                   >  1:379542/1‑251 (MQ=255)                                                                                                                                                                      gTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGTGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACAGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACg                                                                              >  1:411342/1‑237 (MQ=255)                                                                                                                                                                                             gTTCCAGACTTTGGCGCGTGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACAGAGAATTTCCCCGCCGCCAGCCGTGCTTTGGCATCGGCAATGGCCTCCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCCTTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCATCCCCACCTTTCCCCAGCCGTGAACGGGGACGCCCAGACCGTCCTTCATCAGCTATGGCGACAccc                                            >  2:276753/1‑248 (MQ=255)                                                                                                                                                                                                ccAGACTTTGGCGCGTGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACAGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCtt                                      <  1:491459/251‑1 (MQ=255)                                                                                                                                                                                                      tttGGCTCGTGAGAAGAGGATTTCACCGCAAGGTTCCTGGATCTGAACAGAGTCTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGTCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCGCCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTac                                  <  1:51754/249‑1 (MQ=255)                                                                                                                                                                                                      tttGGCGCGTGAGAAGTGTATTTCTTCGCAAGGTTCTTGGATCTTAACATAGACTTTCCCCGCCGCCATCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCCCTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTaca                                 <  2:413299/250‑1 (MQ=255)                                                                                                                                                                                                      tttGGCGCGTGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACAGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGTTTTGGCGAAACCCAGGCTTCTacac                                >  2:357824/1‑251 (MQ=255)                                                                                                                                                                                                          gcgcgtgagaagtgtttgcccgcGCAAGGTTCCTGAATTTTAACAGAGACTTCCCCCGCCCCCAGCAGTGCTGTGGCATCGGCAATTGCCTGCGCTTTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGTCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTTACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTc                            <  1:317444/229‑1 (MQ=255)                                                                                                                                                                                                                         aTTTCATCGCAAGGTTCCTGGAACTTAACAGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTCCAGGGAACGGGGCAGCCAAGACCGTTCTTAAACAGATTTGGCAAAAACCAGGCGTCTACACGCTCATCCGGACCTTcc               >  2:108122/1‑249 (MQ=255)                                                                                                                                                                                                                         aTTTCATCGCAAGGTTCCGGGATTTTAACAGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAg             <  1:356851/251‑1 (MQ=255)                                                                                                                                                                                                                             caccGCAAGGTTCCTGGATCTTAACAGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCt         <  1:45728/248‑1 (MQ=255)                                                                                                                                                                                                                                    agttcccTGGATCTTAACATATATTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCgcag  <  1:809/246‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                         GCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGTGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACGGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAG  >  NC_000913/3256056‑3256533

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑