Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,871,153 T→G N51T (AAC→ACC)  truD ← tRNA pseudouridine(13) synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,871,1530TG91.9% 113.0 / ‑2.4 37N51T (AAC→ACC) truDtRNA pseudouridine(13) synthase
Reads supporting (aligned to +/- strand):  ref base T (2/1);  new base G (21/13);  total (23/14)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.14e-01

CGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCTTGTGCCGCGCATACTCCAGCACCTGGCAGCCTTCCAGTTGAAAGGCGCTCAGATCGGGCATTTCCTTGCCCGGCACGCGAGCGCATAACCACTGTTCCGTAACAGCATGTTTGTCTTTTTGCCCAGCGAAGCTGACTT‑CACGGGCATGAATTTTCAGGAATTTCGCCAG‑TGCATCCGCCACAAAACGGGTATTGCAGCCGTTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGT‑CTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCTTCACAGGCAATCCCTTCCCCACGTCCGGTAAATCCCAGTTTTTCCGTAGTAGTGGCTTTCAC  >  NC_000913/2870917‑2871396
                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                    
cGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCTTGTGCCGCGCATACTCCAGCACCTGGCAGCCTTCCAGTTGAAAGGCGCTCAGATCGGGCATTTCCTTGCCCGGCACGCGAGCGCATAACCACTGTTCCGTAACAGCATGTTTGTCTTTTTGCCCAGCGAAGCTGACTT‑CACGGGCATGAATTTTCAGGAATTTCGCCAG‑TGCATCCGCCACAAACCGGGTATTGCAGCCGGTTTTGAGGATTCt                                                                                                                                                                                                                                         >  1:407081/1‑250 (MQ=255)
                  cTAAACGCAGCTTGCGCTTGTGCCGCGCATACTCCAGCACCTGGCAGCCTTCCAGTTGAAAGGCGCTCAGATCGGGCATTTCCTTGCCCGGCACGCGAGCGCATAACCACTGTTCCGTAACAGCATGTTTGTCTTTTTGCCCAGCGAAGCTGACTT‑CACGGGCATGAATTTTCAGGAATTTCGCCAG‑TGCATCCGCCACAAAACGGGTATTGCAGCCGTTTTTGAGGATTCTAACCAGAATATGCTCACCt                                                                                                                                                                                                                      >  2:25187/1‑251 (MQ=255)
                                  cTTGTGCCGCGCATACTCCAGCACCTGGCAGCCTTCCAGTTGAAAGGCGCTCAGATCGGGCATTTCCTTGCCCGGCACGCGAGCGCATAACCACTGTTCCGTAACAGCATGTTTGTCTTTTTGCCCAGCGAAGCTGACTT‑CACGGGCATGAATTTTCAGGAATTTCGCCAG‑TGCATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCa                                                                                                                                                                                                       >  1:68362/1‑250 (MQ=255)
                                    tgtgCCCCTCATACTCCAGCACCTGGCAGCCTTCCAGTTGAAAGGCGCTAAGATCGGGCATTTCCTTGCCCGGCACGCGGGCGCATAACCACTGTTCCGTAACAGCATGTTTGTCTTTTTGCCCAGCGAAGCTGACTT‑CACGGGCATGAATTTTCAGGAATTTCGCCAG‑TGCATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCaa                                                                                                                                                                                                      <  1:308155/249‑1 (MQ=255)
                                             cATACTCCAGCACCTGGCAGCCTTCCAGTTGAAAGGCGCTCAGATCGGGCATTTCCTTGCCCGGCACGCGAGCGCATAACCACTGTTCCGTAACAGCATGTTTGTCTTTTTGCCCAGCGAAGCTGACTT‑CACGGGCATGAATTTTCAGGAATTTCGCCAG‑TGCATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGTCTCAAAGCCCAAATCt                                                                                                                                                                                           >  2:129646/1‑251 (MQ=255)
                                             cATACTCCAGCACCTGGCAGCCTTCCAGTTGAAAGGCGCTCAGATCGGGCATTTCCTTGCCCGGCACGCGAGCGCATAACCACTGTTCCGTAACAGCATGTTTGTCTTTTTGCCCAGCGAAGCTGACTT‑CACGGGCATGAATTTTCAGGAATTTCGCCAG‑TGCATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCt                                                                                                                                                                                           >  1:422844/1‑251 (MQ=255)
                                                aCTCCAGCACCTGGCAGCCTTCCAGTTGAAAGGCGCTCAGATCGGGCATTTCCTTGCCCGGCACGCGAGCGCATAACCACTGTTCCGTAACAGCATGTTTGTCTTTTTGCCCAGCGAAGCTGACTT‑CACGGGCATGAATTTTCAGGAATTTCGCCAG‑TGCATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTcc                                                                                                                                                                                        >  1:247278/1‑251 (MQ=255)
                                                               aGCCTTCCAGTTGAAAGGCGCTCAGATCGGGCATTTCCTTGCCCGGCACGCGAGCGCATAACCACTGTTCCGTAACAGCATGTTTGTCTTTTTGCCCAGCGAAGCTGACTT‑CACGGGCATGAATTTTCAGGAATTTCGCCAG‑TGCATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGT‑Ct                                                                                                                                                                        >  1:133872/1‑251 (MQ=255)
                                                                 cctccatgtaaaagggCGCTAAGATCGGGCATTCCCTTGCCCGGCACGCGAGCGCATAACCATGGTCCCGTAACAGCATGTTGGTTTTTTTGCCCAGCGAAGCTGATTA‑CCCGGGAATGAATTTTCAGGAATTTCGCCGG‑TGCATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGT‑CTTc                                                                                                                                                                      <  1:418990/237‑1 (MQ=255)
                                                                      cAGTTGAAAGCCGCTAAGATCGGGCATTCCCTTGCCCGGCCCGCGAGCGCAAAACCTCTGTCCCGTAACAGCATGTTTGTTTTTTTCCCCAGCGAAGCTGACTT‑CACGGGCATGAATTTTCAGGAATTTCCCCAG‑TGCATCCGCCACAAAACGGGTATTGCAGCCGTTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGT‑CTTCCGGAt                                                                                                                                                                 <  1:25187/251‑1 (MQ=255)
                                                                       aGTTGAAAGGCGCTCAGATCGGGCATTTCCTTGCCCGGCACGCGAGCGCATAACCACTGTTCCGTAACAGCATGTTTGTCTTTTTGCCCAGCGAAGCTGACTT‑CACGGGCATGAATTTTCAGGAATTTCGCCAG‑TGCATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGT‑CTTCCGGAtt                                                                                                                                                                >  1:221595/1‑251 (MQ=255)
                                                                        gTTGAAAGGCGCTCAGACCGGGCATTCCCTTGCCCGGCCCGCGAGCGCATACCCACTTTTCCGTAACAGCATGTTTGTCTTTTTGCCCAGCGAAGCTGACTT‑CCCGGGCATGAATTTTCAGGAATTTCGCCAG‑TGCATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGT‑CTTCCGGATTg                                                                                                                                                               <  1:129646/251‑1 (MQ=255)
                                                                                                 ttCCTTGCCCGGCACGCGAGCGCATAACCACTGTTCCGTAACAGCATGTTTGTCTTTTTGCCCAGCGAAGCTGACTT‑CACGGGCATGAATTTTCAGGAATTTCGCCAG‑GGCATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACATTCACCATCAGGCTCAAAGCACACATCTTCCACCACCACAA‑GTACTTCCGGATTGGCTTTCAGCAGCCCGGTGCCttacg                                                                                                                                      >  1:186321/1‑248 (MQ=255)
                                                                                                  tCCTTGCCCGGCACGCGAGCGCATAACCACTGTTCCGTAACAGCATGTTTGTTTTTTTGCCCAGCGAAGCTGACTT‑CACGGGAATGAATTTTAAGGAATTTCGCCAG‑TGCATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGT‑CTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCg                                                                                                                                      <  1:432212/250‑1 (MQ=255)
                                                                                                          cGGCACGCGAGCGCATAACCACTGTCCCGTAACAGCATGTTTGTCTTTTTGCCCAGCGAAGCTGACTT‑CACGGGCATGAATTTTCAGGAATTTCGCCAG‑TGCATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGT‑CTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGt                                                                                                                             <  1:284387/251‑1 (MQ=255)
                                                                                                               ggcgatcgataaaCCTCTGTTCCGTAACTGTATGTTTGTTTGTTGGCCCAGAAAAG‑TGGCTTTCACGGGCTAGAATTTTAATGATTTTCGCCAG‑TGCATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGT‑CTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAgg                                                                                                                        <  1:7425/240‑1 (MQ=255)
                                                                                                               cgcgagccattacccACTTTTCCTTAACAGCTTTTTTGTTTTTTCGCCCAGCGAAGCTGACTT‑CACGGGCATGATTTTTCAGGAATTCCGCCAG‑TGCATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGT‑CTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAgg                                                                                                                        <  1:419088/238‑1 (MQ=255)
                                                                                                                     cgtaTAACCACTTTCCCGAAACACCATTTTTGTCTTTTCGCCGAGCGAAGCTGATTT‑CCCGGGAATGAATTTCAAGGAATTACGCTAG‑TGCATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGT‑CTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTg                                                                                                                  <  2:455477/248‑1 (MQ=255)
                                                                                                                                   tCCGTAACAGCATTTTTTTCTTTTTCCCCCGCGAAGCTAATTT‑CCCGTGATTGAATTTTCAGGAATTCCGCCGG‑TGCATCCGCCACAAAACGGGTATTGCAGCCGGTTTTTAGGATTCTAACCAGAATATGCTCTCCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGT‑CTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCAAACt                                                                                                     <  1:448948/250‑1 (MQ=255)
                                                                                                                                         aCAGCATGTTTGTCTTTTTGCCCAGCGAAGCTGACTT‑AACGGGCATGAATTTTAAGGAATTTCGCCAG‑TGCATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTAAAAGCCCAACTCTTCCACCACCACAAAGT‑CTTCCGGATTGGCTTTCAGCAGCCCGGGGCCTTGCGGTTTACCGTGGAGGTAAGAGAGATTATCAAACtcattcat                                                                                              >  1:282770/1‑251 (MQ=255)
                                                                                                                                          cAGCATGTTTGTCTTTTTGCCCAGCGAAGCTGACTT‑CACGGGCATGAATTTTCAGGAATTTCGCCAG‑TGCATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGT‑CTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGCTTATCAAACTCAATCAtt                                                                                             >  2:127200/1‑251 (MQ=255)
                                                                                                                                                      tttttctcccAGCGAAGTTGACTT‑CACGGGTATGAATTTTCAGAAATTTCGCCAT‑TTCTTCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGT‑CTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTa                                                                                  <  1:72639/243‑1 (MQ=255)
                                                                                                                                                          ttGCACAGCGAAGCGTACTT‑CACGGGTATAAATTTGCAG‑AATTTTCCCAGGTTCATCCGCCACAAAACGGGGATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGT‑CTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGa                                                                              <  1:462540/250‑1 (MQ=255)
                                                                                                                                                                    aaGCTGACTT‑CACGGGCATGAATTTTCAGGAATTTCGCCAG‑TGCATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAACGT‑CTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCAACCTCAATCATTTTGTTGCCTTAATGAGTAGCGCCAcc                                                                   >  1:473867/1‑251 (MQ=255)
                                                                                                                                                                          aCTT‑CACGGGCATGAATTTTCAGGAATTTCGCCAG‑TGCATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGT‑CTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCACACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCTTc                                                              >  2:459626/1‑250 (MQ=255)
                                                                                                                                                                           actccccGGGAATGAATTTTCAGGATTTTCGCCAG‑TGCATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGT‑CTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCTTca                                                             <  1:373918/245‑1 (MQ=255)
                                                                                                                                                                                            tttCAGGAATTTCGCCAG‑TGCATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGT‑CTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCTTCACAGGCAATCCCTTcccc                                            >  1:9286/1‑251 (MQ=255)
                                                                                                                                                                                                   aaTTTCGCCAG‑TGCATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGT‑CTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCTGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCTTCCCAGGCAATCCCTTCCCCACGTCCg                                     >  2:333232/1‑251 (MQ=255)
                                                                                                                                                                                                         gCCAG‑TGCATCCGCCACAAAACGGGGATTGCAGTCGGCTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGATCAAAGCCCAAATCTTCCACCACCACAACGT‑CTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGCGGTAAGTGAGATTATCAAACTCACTCATTTTGTTGCCTTAATGAGTAGCGCCACCGCTTCACAGGCAATCCCTTCCCCACGTCCGCTAAAt                               >  2:292483/1‑251 (MQ=255)
                                                                                                                                                                                                                        cacaAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGT‑CTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCTTCACAGGCAATCCCTTCCCCACGTCCGGTAAATCCCAGTTTTTCCgt                 >  1:463892/1‑251 (MQ=255)
                                                                                                                                                                                                                          caAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGT‑CTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCTTCACAGGCAATCCCTTCCCCACGTCCGGTAAATCCCAGTTTTTCCgt                 >  1:157887/1‑249 (MQ=255)
                                                                                                                                                                                                                           aaaaCGGGTCTTGCATCCGTTTTTGAGGATTCTAACCAGAATATGCTCAACTTCATCAACAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGT‑CTTCCGAATTGGCTTTCAGCACCCCGGTGCCTTGCGGTTTACCGTGGCGGTAAGTGAGATTATCCAACTCAATTATTTTGTTGCCTTAATGAGTAGAGACAATACTTCACAGGCAATCCCTTCCGCACGTACGGTAATGTCCAGATTTTCCgtagt              >  2:382918/1‑251 (MQ=255)
                                                                                                                                                                                                                           aaaaCGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGT‑CTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCTTCACAGGCAATCCCTTCCCCACGTCCGGTAAATCCCAGTTTTTCCgtagt              >  1:494668/1‑251 (MQ=255)
                                                                                                                                                                                                                           aaaaCGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGT‑CTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCTTCACAGGCAATCCCTTCCCCACGTCCGGTAAATCCCAGTTTTTCCgtagt              <  1:459626/251‑1 (MQ=255)
                                                                                                                                                                                                                           aaaaCGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGT‑CTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCTTCACAGGCAATCCCTTCCCCACGTCCGGTAAATCCCAGTTTTTCCgtagt              >  1:320336/1‑251 (MQ=255)
                                                                                                                                                                                                                               cGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGT‑CTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCTTCACAGGCAATCCCTTCCCAACGTCCGGTAAATCCCAGTTTTTCCGTAGTAGTg          >  1:240735/1‑251 (MQ=255)
                                                                                                                                                                                                                                       gCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGT‑CTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCTTCACAGGCAATCCCTTCCCCACGTCCGGTAAATCCCAGTTTTTCCGTAGTAGTGGCTTTCAc  >  1:403760/1‑251 (MQ=255)
                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                    
CGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCTTGTGCCGCGCATACTCCAGCACCTGGCAGCCTTCCAGTTGAAAGGCGCTCAGATCGGGCATTTCCTTGCCCGGCACGCGAGCGCATAACCACTGTTCCGTAACAGCATGTTTGTCTTTTTGCCCAGCGAAGCTGACTT‑CACGGGCATGAATTTTCAGGAATTTCGCCAG‑TGCATCCGCCACAAAACGGGTATTGCAGCCGTTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGT‑CTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCTTCACAGGCAATCCCTTCCCCACGTCCGGTAAATCCCAGTTTTTCCGTAGTAGTGGCTTTCAC  >  NC_000913/2870917‑2871396

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑