| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,817,197 | G→A | R103C (CGC→TGC) | yqaB ← | fructose‑1‑phosphate phosphatase YqaB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,817,197 | 0 | G | A | 98.5% | 200.1 / ‑6.0 | 67 | R103C (CGC→TGC) | yqaB | fructose‑1‑phosphate phosphatase YqaB |
| Reads supporting (aligned to +/- strand): ref base G (1/0); new base A (30/35); total (32/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.70e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.45e-01 | |||||||||||
ACATCCACGGCGTCCATGCCTGCTGCACGGGCCGCCTGAATACCGAAATCGGCATCTTCAAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTCAC‑TCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATC‑AACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGCCTGATTCAGCTCAATAATTGCCTGAGCAATACGCCAGGTGGGCGATCCATTAAGCGCAATCATCGCCTGAATATCGTACTGAAGACCGTAGTGCCCTAATACTTCGCGCCACGCTTTACGGTGCGT > NC_000913/2816950‑2817440 | acagccACGGCGTCCATGCCTGCTGCACGGGCCGCCTGAATACCGAAATCGGCATCTTCAAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTCAC‑TCCCCGTTCCTACAGCCATTGGGcaacg < 1:400695/247‑1 (MQ=255) cACGGCGTCCATGCCTGCTGCACGGGCCGCCTGAATACCGAAATCGGCATCTTCAAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCACCGACGGCGGCGTCAAAATAATGGCGTAATCCCAGATGCGCCCGCAATGCCTCAGCGATGGAGCTTTCAC‑TCCCCGTTCCTCCCGCCATTGGGTAACGACCAt > 2:117716/1‑251 (MQ=255) ggCGTCCATGCCTGCTGCACGGGCCGCCTGAATACCGAAATCGGCATCTTCAAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTCAC‑TCCCCGTTCCTACAGCCATTGGGCAACGACCATGcc > 1:401875/1‑251 (MQ=255) cGTCCAGGCCTGTCGCACGTGCCGCCTTAATACCGAAATCGGCATCTTCAAAGACCACACACTGCGTCGGTGGCACGCCCATACGCTGCGCGCACAACAAAAATGTGGCTGGCGCGGGGTTATGGTGTTTTACGTGATCGGCAGCGTCGACGGCGTCTAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCGCAGCGAAGGCGATTTCAC‑GCCCCTTTCCGACAGCCGTTGGGCAACGACCATGCCaa < 2:346020/251‑1 (MQ=255) cATGCCTGCTGCACGGGCCGCCTGAATACCGAAATCGGCATCTTCAAAGACCACTCACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGCCGCCGGCGCCAAAATATTGGCTTAATCCCAGGGGCCCCAGCCATGCCTCCGCGTGGGCGCTTTCAC‑TCCCCGTTCCTACACCAATTGGGCAACCCCCTTGCCAACttt > 2:359539/1‑251 (MQ=255) gCCTGCTGCACGGGCCGCCTGAATACCGAAATCGGCATCTTCAAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTCAC‑TCCCCGTTCCTACAGCCATTGGGCAACGACCATGCCAACTTTTca < 2:77927/251‑1 (MQ=255) ccTGCTGCACGGGCCGCCTGAATACCGAAATCGGCATCTTCAAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTCAC‑TCCCCGTTCCTACAGCCATTGGGCAACGACCATGCCAACTTTTcac < 2:238412/251‑1 (MQ=255) cACGGGCCGCCTGTCTACCGAAATCGGCATCTTCAAAGACCACACACAGCGTCGGTTGCACTCCCATCCGCTGCGCGCACAACAAAAATGTGTCCGGCGCGGGTTTATGGTGTTTGACGTCTTCGGCAGCGTCGACGGCCTCAAACTAATGTGGGCATCCCAGGGGCGCCAGAACTGCCTCAGTGCTGGATCTT‑CACATCCACGATCCTACAGCCATTGGCCAACGCCAATGCCATCTTTTCACCTCATc > 2:195913/1‑250 (MQ=255) ggCCGCCTGAATTCCGAAATCGGCATCTTCAAAGACCACCCACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCTTAATCCCAGTTGCGCCAGCAATGCCTCAGCGATGGCGCTTTCAC‑TCCCCGTTCCTACAGCCATTGGGCAACGACCATGCCAACTTTTCACCACATc‑aacaa < 1:241162/251‑1 (MQ=255) gAATACCGAAATCGGCATCTTCAAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTCTGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTCAC‑TCCCCGTTCCTACAGCAATTGGGCGACGACCATGCCAACTTTTCACCACATC‑AACCAGAGGAAGc > 2:216410/1‑251 (MQ=255) gAATACCGAAATCGGCATCTTCAAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCAGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTCAC‑TCCCCGTTCCTACAGCCATTGGGCAACGACCATGCCAACTTTTCACCACATC‑AACAAGAGGaa < 1:7649/249‑1 (MQ=255) gAATACCGAAATCGGCATCTTCAAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAAGGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTCAC‑TCCCCGTTCCTACAGCCATTGGGCAACGACCATGCCAACTTTTCACCACATC‑AACAAGAGGAAg < 2:401875/250‑1 (MQ=255) aaTACCGAAATCGGCATCTTCAAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTCAA‑TCCCCGTTCCTACAGCCATTGGGCAACGACCATGCCAACTTTTCACCACATC‑AACAAGAGGAAGCg > 1:237151/1‑251 (MQ=255) aCCGAAATCGGCATCTTCAAAGACCACACCCTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCATCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTCAC‑TCCCCGTTCCTACAGCCATTGGGCAACGACCATGCCAACTTTTCACCACATC‑AACAAGAGGAAGCGGtt < 1:20339/251‑1 (MQ=255) cGGCATCTTCAAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTCAC‑TCCCCGTTCCTACAGCCATTGGGCAACGACCATGCCAACTTTTCACCACATC‑AACAAGAGGAAGCGGTTCGACGCt < 1:299911/250‑1 (MQ=255) cctCAAAGACCACACACCTCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAAAGTGTCTGGCGCGGGTTTATGGTTTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTCAC‑TCCCCGTTCCTACAGCCATTGGGCAACGACCATGCCAACTTTTCACCACATC‑AACAAGAGGAAGCGGTTCGACGCTATCcag < 1:30394/248‑1 (MQ=255) tCAAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAAGGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTCAC‑TCCCCGTTCCTACAGCCATTGGGCAACGACCATGCCAACTTTTCACCACAGC‑AACAAGAGGAAGCGGTTCGACGCTATCcag < 2:62128/248‑1 (MQ=255) tCAAAGACCACACACTGAGTCGGCTGCACGCCCATACGCTGCGCGCACACCAACAATGTGTCTCGCGCGGGATTATGGTGTTTGACGTGATCGCCAGCGACGACGGCGTCCAAATAATGGCGTAATCCCAGGCGCGCCAGCCATGCCTCAGCGATGGCGCTTTTAC‑TCCCCGCTCCACCAGCCCTTGGGACACGACCATGCCAACTTTTCACCACATC‑AACAAGAGGAAGCGGTTCGAAGCTATCcagc > 2:245497/1‑249 (MQ=255) cAAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTCAC‑TCCCCGTTCCTACAGCCATTGGGCAACGACCATGCCAACTTTTCACCACATC‑AACAAGAGGAAGCGGTTCGACg > 1:193137/1‑239 (MQ=255) cAAAGACCACACACGGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTCAC‑TCCCCGTTCCTACAGCCATTGGGCAACGACCATGCCAACTTTTCACCACATC‑AACAAGAGGAAGCGGTTCGACg < 2:193137/239‑1 (MQ=255) aGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTCAC‑TCCCCGTTCCTACAGCCATTGGGCAACGACCATGCCAACGTTTCACCACATC‑AACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATa < 1:476012/251‑1 (MQ=255) aGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGAAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTCAC‑TCCCCGTTCCTACAGCCATTGGGCAACGACCATGCCAACTTTTCACCACATC‑AACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATa < 1:159099/251‑1 (MQ=255) gACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGAGCCCAACAAAACTATGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGCAGGCGTCAAAATAATGGCGTAATCCCAGGGGCGCCAGCAATGCCTCAGCGATGGCGCTTTCCC‑TCCCCAGTCCTACCGCCATTGGGCAACGACCATGCCAACTTTTCACCACATC‑AACAATAGGAAGCGGTTAGCAGCTATCCGCCAGCATAc > 2:473039/1‑251 (MQ=255) gACCACACACTGCGTCGGTTGCAAGCCCATACCCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGCGTTTGACGTGAACGGCAGCGACGACGGCGTCAAAATAATGGCGGAATCCCCGGAGCGCCAGCAATGCCTAAGCGATGGCGCTTTCAC‑TCCACGTGCATACAGCCAATGGGCAACGACCATGCCAACTTTTCACCACATCCAACA‑GAGCAAGCGGTTCGGCGCAATCCAGCAGCATa > 2:28087/1‑250 (MQ=255) caccgcggcGGTTGCACGCCCATACGCTGCGCGCACAAAAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTCAC‑TCCCCGTTCCTACAGCCATTGGGCAACGACCATGCCAACTTTTCACCACATC‑AACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTAc < 2:444120/243‑1 (MQ=255) ggttCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTCAC‑TCCCCGTTCCTACAGCCATTGGGCAACGACCATGCCAACTTTTCACCACATC‑AACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGc < 2:438154/247‑1 (MQ=255) ggTCGGTTGCACGCCCATACGCTGCGAGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTCAC‑TCCCCGTTCCTACAGCCATTGGGCAACGACCATGCCAACTTTTCACCACATC‑AACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCtt < 2:237300/250‑1 (MQ=255) gTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTCAC‑TCCCCGTTCCTACAGCCATTGGGCAACGACCATGCCAACTTTTCACCACATC‑AACAAGAGGAAGCGGTTCGTCGCTATCCAGCAGCATACTTCTTACTGCTTc < 1:143029/251‑1 (MQ=255) tGCACGCCCATACGCTGCGCGCACCACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTCAC‑TCCCCGTTCCTACAGCCATAGGGCAACGACCCTGCCACCTTTTACCCACATC‑AACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGtttt > 2:293729/1‑251 (MQ=255) gcgcACAACAAAAATGTGGCTGGCGCGGGTTTATGGGGTTTGACGGGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTCAC‑TCCCCGTTCCTACAGCCATTGGGCAACGACCATGCCAACTTTTCACCACATC‑AACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATg < 2:362973/251‑1 (MQ=255) cgcACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTCAC‑TCCCCGTTCCTACAGCCATTGGGCAACGACCATGCCAACTTTTCACCACATC‑AACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGc > 1:155893/1‑251 (MQ=255) gcACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTCAC‑TCCCCGTTCCTACAGCCATTGGGCAACGACCATGCCAACTTTTCACCACATC‑AACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCg < 1:211234/251‑1 (MQ=255) caacaaAGATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCTGCAGCGACTACGGCGTCAAAATAATGGCTTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTCCC‑GCCCCGTTTCTACATCCATTGGGCAACGACCATGCCAACTTTTCACCACATC‑AACAAGAGGAAGCGGTTCGACGCTTTCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCGGGt < 1:206463/251‑1 (MQ=255) caaAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTCAC‑TCCCCGTTCCTACAGCCATTGGGCAACGACCATGCCAACTTTTCACCACATC‑AACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCg > 1:411728/1‑250 (MQ=255) aacaaTGGGTCTGGCGCGGGTTTATGGTGTTTGAAGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTATTCCCAGGTGCGCCAGCAATGCCTCAGCGAAGGAGCTTTCAC‑TCCCCGATCCTACAGCCATTGGGCAACGACCATTCCAACTTTTCACCACATC‑CACAAGAGGAAGGGGATCGAGGCTATCTAGCAGCATACTTCTTACTGCTTTGGTTTTTTCACGCGCTaccacctgcagagcg > 2:392644/4‑235 (MQ=255) aTGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTCAC‑TCCCCGTTCCTACAGCCATTGGGCAACGACCATGCCAACTTTTCACCACATC‑AACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCg > 1:180821/1‑245 (MQ=255) aTGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTCAC‑TCCCCGTTCCTACAGCCATTGGGCAACGACCATGCCAACTTTTCACCACATC‑AACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCg < 1:285235/251‑1 (MQ=255) gggTTTATTGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTCAC‑TCCCCGTTCCTACAGCCATTGGGCAACGACCATGCCAACTTTTCACCACATC‑AACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGCCTGATTCAGCt > 1:154195/1‑251 (MQ=255) gggTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTCAC‑TCCCCGTTCCTACAGCCATTGGGCAACGACCATGCCAACTTTTCACCACATC‑AACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGCCTGATTCAGCt > 2:156213/1‑251 (MQ=255) ttATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTCAC‑TCCCCGTTCCTACAGCCATTGGGCAACGACCATGCCAACTTTTCACCACATC‑AACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGCCTGCTTCAGCTCaat > 2:500058/1‑251 (MQ=255) tttGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTCAC‑TCCCCGTTCCTACAGCCATTGGGCAACGACCATGCCAACTTTTCACCACATC‑AACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGCCTGATTCAGCTCAATAATTGCCt > 1:422886/1‑251 (MQ=255) tGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTCAC‑TCCCCGTTCCTACAGCCATTGGGCAACGACCATGCCAACTTTTCACCACATC‑AACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGCCTGATTCAGCTCAATAATTGCCTg > 1:324727/1‑250 (MQ=255) gTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAACCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTCAC‑CCCCCGTTCCTACAGCCATTGGGCAACGACCATGCCAACTTTTCACCACATC‑AACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGCCTGATTCAGCTCAATAATTGCCTGAGCaa < 2:175549/251‑1 (MQ=255) ggCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTCAC‑TCCCCGTTCCTACAGCCATTGGGCAACGACCATGCCAACTTTTCACCACATC‑AACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGCCTGATTCAGCTCAATAATTGCCTGAGCAATACGcc > 2:14876/1‑251 (MQ=255) aGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTCAC‑TCCCCGTTCCTACAGCCATTGGGCAACGACCATGCCAACTTTTCACCACATC‑AACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGCCTGATTCAGCTCAATAATTGCCTGAGCAATACGCCAgg < 2:180821/251‑1 (MQ=255) aGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTCAC‑TCCCCGTTCCTACAGCCATTGGGCAACGACCATGCCAACTTTTCACCACATC‑AACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGCCTGATTCAGCTCAATAATTGCCTGAGCAATACGCCAgg < 1:160370/251‑1 (MQ=255) acgGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTCAC‑TCCCCGTTCCTACAGCCATTGGGCAACGACCATGCCAACTTTTCACCACATC‑AACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGCCTGATTCAGCTCAATAATTGCCTGAGCAATACGCCAGGTGGGCg > 1:188029/1‑250 (MQ=255) cGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTCAC‑TCCCCGTTCCTACAGCCATTGGGCAACGACCATGCCAACTTTTCACCACATC‑AACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGCCTGATTCAGCTCAATAATTGCCTGAGCAATACGCCAGGTGGGCGATcc > 1:110507/1‑250 (MQ=255) gTCAAAATAATGGCGTAATCCCAGGTGCGCCCGCAATGCCTCAGCGATGGCGCTTTCAC‑GCCCCGTTCCTACAGCCATTGGGCAACGACCATGCCAACGTTTCACCACATC‑AACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGCCTGATTCAGCTCAATAATTGCCTGAGCAATACGCCAGGTGGGCGATCCAt < 1:448061/251‑1 (MQ=255) aaaTAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTCAC‑TCCCCGTTCCTACAGCCATTGGGCAACGACCATGCCAACTTTTCACCACATC‑AACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGCCTGATTCAGCTCAATAATTGCCTGAGCAATACGCCAGGTGGGCGATCCATTa > 1:352874/1‑249 (MQ=255) aataatGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTCAC‑TCCCCGTTCCTACAGCCATTGGGCAACGACCATGCCAACTTTTCACCACATC‑AACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGCCTGATTCAGCTCAATAATTGCCTGAGCAATACGCCCGGTGGGCGATCCATTAAgc > 2:381744/1‑251 (MQ=255) gCGTAAGCCCAGGTGCGCCAGCAATGCCTCAGCGTTGGCGCTTTCAC‑CCCCCGTTCCTACAGCCATTGGGCAACGACCATGCCAACTTTTCACCACATC‑AACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGCCTGATTCAGCTCAATAATTGCCTGAGCAATACGCCAGGTGGGCGATCCATTAAGCGCAatca < 2:110507/251‑1 (MQ=255) cccAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTCAC‑TCCCCGTTCCTACAGCCATTGGGCAACGACCATGCCAACTTTTCACCACATC‑AACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGCTCGGCCTGATTCAGCTCAATAATTGCCTGAGCAATACGCCAGGTGGGCGATCCATTAAGCGCAATCATCGCCTg > 2:158914/1‑251 (MQ=255) ccAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTCAC‑TCCCCGTTCCTACAGCCATTGGGCAACGACCATGCCAACTTTTCACCACATC‑AACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGCCTGATTCAGCTCAATAATTGCCTGAGCAATACGCCAGGTGGGCGATCCATTAAGCGCAATCATCGCCTg > 1:476342/1‑250 (MQ=255) ccAGCAATGCCTCAGCGATGGCGCTTTCAC‑TCCCCGTTCCTACAGCCATTGGGCAACGACCATGCCAACTTTTCACCACATC‑AACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGCCTGATTCAGCTCAATAATTGCCTGAGCAATACGCCAGGTGGGCGATCCATTAAGCGCAATCATCGcagatcggaagagc < 1:26467/251‑14 (MQ=255) cAGCAATGCCCCAGCGATGGCGCTTTCAC‑TCCCCGTTCCTACAGCCATTGGGCAACGACCATGCCAACTTTTCACCACATC‑AACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGCCTGATTCAGCTCAATAATTGCCTGAGCAATACGCCAGGTGGGCGATCCATTAAGCGCAATCATCGCCTGAATATCGTACt < 2:352874/251‑1 (MQ=255) gCCTCAGCGATGGCGCTTTCAC‑TCCCCGTTCCTACAGCCATTGGGCAACGACCATGCCAACTTTTCACCACATC‑AACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATAGGCCTGATTCAGCTCAATAATTGCATGAGCAATACGCCAGGGGGGCGCTCCATTAAGCGCAATCATCGCCGGAATATCGTACGGAAGAcc > 2:140006/1‑251 (MQ=255) aGCGATGGCGCTTTCAC‑TCCCCGTTCCTACAGCCATTGGGCAACGACCATGCCAACTTTTCACCACATC‑AACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGCCTGATTCAGCTCAATAATTGCCTGAGCAATACGCCAGGTGGGCGATCCATTAAGCGCAATCATCGCCTGAATATCGTACTGAAGACCGTAGt < 1:291354/251‑1 (MQ=255) gATGGCGCTTTCAC‑TCCCCGTTCCTACAGCCATTGGGCAACGACCATGCCAACTTTTCACCACATC‑AACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGCCTGATTCAGCTCAATAATTGCCTGAGCAATACGCCAGGTGGGCGATCCATTAAGCGCAATCATCGCCTGAATATCGTACTGAAGACCGTAGTGcc > 2:122919/1‑251 (MQ=255) gcTTTCAC‑TCCCCGTTCCTACAGCCATTGGGCAACGACCATGCCAACTTTTCACCACATC‑AACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGCCTGATTCAGCTCAATAATTGCCTGAGCAATACGCCAGGTGGGCGATCCATTAAGCGCAATCATCGCCTGAATATCGTACTGAAGACCGTAGTGCCCTAATa < 1:87137/251‑1 (MQ=255) ttCAC‑CCCCCGTTCCTACAGCCATTGGGCAACGACCATGCCAACTTTTCACCACATC‑AACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGCCTGATTCAGCTCAATAATTGCCTGAGCAATACGCCAGGTGGGCGATCCATTAAGCGCAATCATCGCCTGAATATCGTACTGAAGACCGTAGTGCCCTAATAc < 2:496755/249‑1 (MQ=255) ccAC‑TCCCCGTTCCTACAGCCAGTGGGCAACGACCATGCCAACTTTTCACCACACC‑AACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATATTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGCCTGATTCAGCTCAATAATTGCCTGAGCAATACGCCAGGTGGGCGATCCATTAAGCGCAATCATCGCCTGAATATCGTACTGAAGACCGTAGTGCCCTAATACTTc < 2:400170/250‑1 (MQ=255) cAC‑TCCCCGTTCCTACAGCCATTGGGCACCGACCATGCCAACTTTTCACCACAGC‑AACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGCCTGATTCAGCTCAATAATTGCCTGAGCAATACGCCAGGTGGGCGATCCATTAAGCGCAATCATCGCCTGAATATCGTACTGAAGACCGTAGTGCCCTAATACTTcg < 2:411728/251‑1 (MQ=255) cAC‑TCCCCGTTCCTACAGCCATTGGGCAACGACCATGCCAACTTTTCACCACATC‑AACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGCCTGATTCAGCTCAATAATTGCCTGAGCAATACGCCAGGTGGGCGATCCATTAAGCGCAATCATCGCCTGAATATCGTACTGAAGCCCGTAGTGCCCTAATACTTcg > 2:260301/1‑251 (MQ=255) cAC‑TCCCCGTTCCTACAGCCATTGGGCAACGACCATGCCAACTTTTCACCACATC‑AACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGCCTGATTCAGCTCAATAATTGCCTGAGCAATACGCCAGGTGGGCGATCCATTAAGCGCAATCATCGCCTGAATATCGTACTGAAGACCGTAGTGCCCTAATACTTcg > 1:34993/1‑251 (MQ=255) caagaGCCTTTGGGCAACTAACATTCCAACTTTTCCCCACAGC‑AACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATTCTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGCCTGATTCAGCTCAATAATTGCCTGAGCAATACGCCAGGTGGTCGATCCATGAAGCGCAATCAGCGCCTGAATATCTTACTGAAGACCGTAGTGCCCTAATACTTCGCGCCTCGCTTTa < 2:312617/247‑1 (MQ=255) aGCCATTGGGCAACGACCATGCCAACTTTTCACCACATC‑AACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGCCTGATTCAGCTCAATAATTGCCTGAGCAATACGCCAGGTGGGCGATCCATTAAGCGCAATCATCGCCTGAATATCGTACTGAAGACCGTAGTGCCCTAATACTTCGCGCCACGCTTTACGGt < 2:168566/251‑1 (MQ=255) aTTGGGCAACGACCATGCCAACTTTTCACCACATC‑AACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGCCTGATTCAGCTCAATAATTGCCTGAGCAATACGCCAGGTGGGCGATCCATTAAGCGCAATCATCGCCTGAATATCGTACTGAAGACCGTAGTGCCCTAATACTTCGCGCCACGCTTTACGGTGCGt > 1:258297/1‑251 (MQ=255) | ACATCCACGGCGTCCATGCCTGCTGCACGGGCCGCCTGAATACCGAAATCGGCATCTTCAAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTCAC‑TCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATC‑AACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGCCTGATTCAGCTCAATAATTGCCTGAGCAATACGCCAGGTGGGCGATCCATTAAGCGCAATCATCGCCTGAATATCGTACTGAAGACCGTAGTGCCCTAATACTTCGCGCCACGCTTTACGGTGCGT > NC_000913/2816950‑2817440 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |