Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,073	G→A	P91P (CCG→CCA)	thrB →	homoserine kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,073	0	G	A	96.4%	166.6 / NA	55	P91P (CCG→CCA)	thrB	homoserine kinase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (30/23);  minor base C (2/0);  total (32/23)
Fisher's exact test for biased strand distribution p-value = 5.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

CCAGTGCCAATATGAGCGTCGGGTTTGATGTGCTCGGGGCGGCGGTGACACCTGTTGATGGTGCATTGCTCGGAGATGTAGTCACGGTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAACTGGGTAAGCAAATTCCAGTGGCGATGACCCTGGAAAAGAATATGCCGATCGGTTCGGGCTTAGGCTCCAGTGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGCGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGCCGTATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTCGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGT  >  NC_000913/2826‑3311                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                               ccAGGGCCAATATGTGCGTCGGGTTTGATGTGCTCGGGGCGGCGGTGACACCTGTTGATGGTGCATTGCGCGGAGATGTAGTCACGGTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTTGGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATc                                                                                                                                                                                                                                             <  2:486221/251‑1 (MQ=255)  cAGTGCCAATATGAGCGTCGGGTTTGATGTGCTCGGGGCGGCGGTGACACCTGTTGATGGTGCATTGCTCGGAGATGTAGTCACGGTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGACGCTTTTCCGATAAGCTGCCGTCAGAACCCCGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTTGGCAAGCCAATTCCAGTGGCGATGACTCTGGGAAAGAATATGCCAATCg                                                                                                                                                                                                                                            >  2:195378/1‑251 (MQ=255)        cAATATGAGCGTCGGGTTTGATGTGCTCGGGGCGGCGGTGACACCTGTTGATGGTGCATTGCTCGGAGATGTAGTCACGGTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTTGGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCgg                                                                                                                                                                                                                                      >  1:121006/1‑251 (MQ=255)         aaTATGAGCGTCGGGTTTGATGTGCTCGGGGCGGCGGTGACACCTGTTGATGGCGCATTGCTCGGAGATGTAGTCACGGTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTTGGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCggg                                                                                                                                                                                                                                     <  1:176078/251‑1 (MQ=255)            atGAGCGTCGGGTTTGATGTGCTCGGGGCGGCGGTGACACCTGTTGATGGTGCATTGCTCGGAGATGTAGTCACGGTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTTGGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCtt                                                                                                                                                                                                                                  <  2:136238/251‑1 (MQ=255)               aGCGTCGGGTTTGATGTGCTCGGGGCGGCGGTGACACCTGTTGATGGTGCATTGCTCGGAGATGTAGTCACGGTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGGGCGGTTTTGCCAGGAGCTTGGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAgg                                                                                                                                                                                                                               >  1:366256/1‑251 (MQ=255)                       gttggATGTGCTCGGGGCGGCGGTGACACCTGTTGCTGGTGCCTTGCTCGGAGATGGAGTCACGGTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTTGGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTcaagcg                                                                                                                                                                                                                       >  2:217992/5‑246 (MQ=255)                       gattgctgtgCTCGGGGCGGCTGTGACACCTGTTGATGGTGCATTGCTCGGAGATGTAGTCACGGTTGAGGCGGCAGAGGCATTAAGGCGCAACAACATCGGACGCTTTGACGGTAAGCAGCAGGCAGAACCACGGGAGAAAATCTGTTATCAGTGCTGGGAGCGTTTTTGACAGGAGCTTGGCAAGAAACTACCAGTGGCGATGCCTCTGGAAAAGTACATTCCCATCGGCTTGGGCTTAGGGTCCaacg                                                                                                                                                                                                                       >  2:362061/7‑248 (MQ=255)                              gtgCTCGGGGCGGCGGTGACACCTGTTGATGGTGCATTGCTCGGAGATGTAGTCACGGTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTTGGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTc                                                                                                                                                                                                                <  1:100320/251‑1 (MQ=255)                                         ggcggTGACACCTGTTGATGGTGCATTGCTCGGAGATGTAGTCACGGTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTTGGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCgcgg                                                                                                                                                                                                     >  1:144697/1‑251 (MQ=255)                                         ggcggTGACACCTGTTGATGGTGCATTGCTCGGAGATGTAGTCACGGTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTTGGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCgcgg                                                                                                                                                                                                     <  1:195378/251‑1 (MQ=255)                                             gTGACACCTGTTGATGGAGCATTGCTCGGAGATATAGTCACCGTTGAGGCGGCCGCGACTTTCAGCCCCAACAACCTCGGACGCTTTGCCGATACGCCGCCGTCAGAACCACGGGAAAATATAGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTTGGAAAGCCAATTCCCGTGGCGATGACTCTGGCAAAGAATTTGCCAATAGGCTAGGGGTTAGGCTCCAACGCCCGTTCGATGGTCggggcgat                                                                                                                                                                                                 >  2:207515/1‑249 (MQ=255)                                                 cacCTGTTGATGGTGCATTGCTCGGAGATGTAGTCACGGTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGACCGTTTTTGCCAGGAGCTTGGCAAGCAAATTCCATTGGCGATGCCTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATg                                                                                                                                                                                             >  1:269674/1‑251 (MQ=255)                                                                cATTGCTCGGAGATGAAGTCACGGTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGACGCTGTGCCGCTAAGCTGCCGTACGCACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTTGGCAAGCAAATTCCAGGGGCGACGACTTTGGAAAAGAATATGCCAATCGGATCGGGCGTAGGCACCAGCGCCTGTTTGGTGGTCGCGGCGCAGGTTGCGATGAATGTacac                                                                                                                                                                              >  2:313586/1‑251 (MQ=255)                                                                        ggAGATGTAGTCACGGTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTCTCAGTGCTGGGAGCGTTTTTGCCAGGAGCTTGGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGGGGTCGCGGCGCTGTAGGCGATGAATGAacacggtggca                                                                                                                                                                       >  2:85660/1‑243 (MQ=255)                                                                             tGTAGTCACGGTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTTGGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGc                                                                                                                                                                 >  1:299963/1‑251 (MQ=255)                                                                                     cGGTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTTGGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGac                                                                                                                                                         >  1:429023/1‑251 (MQ=255)                                                                                      ggTTGCGGCGGCAGAGAAATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGCACCCCGGGAAAATATCGTTTATCAGTGCTGGGCGCGTTTTTGCCAGGCGCTTGGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGTTGGCGGTGAATGCACACTGTGGCAAGCCGCTTAATGac                                                                                                                                                         >  2:23863/1‑250 (MQ=255)                                                                                          gAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTTGGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCg                                                                                                                                                    >  2:424535/1‑251 (MQ=255)                                                                                              cggcAGAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTTGGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTACTGACACTCGTTTg                                                                                                                                                >  2:136292/1‑251 (MQ=255)                                                                                                 ccgagaCATTCAGGCTCAACAACCTCGGTCGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATTTCGTTTATCATTGCTGGGAGCGTTTTTGCCAGGAGCTTGGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACTCTGTGGCAAGCCGCTTAATGACACTCGTTTGCTg                                                                                                                                             <  2:475592/249‑1 (MQ=255)                                                                                                       cATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTTGGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGATCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTg                                                                                                                                       >  2:259270/1‑251 (MQ=255)                                                                                                        aTTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTTGGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGTGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACTCTCGTTTGCTGGCTTTGa                                                                                                                                      <  1:161723/251‑1 (MQ=255)                                                                                                                     aacCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCAAGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTTGGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGGGGTCGCGGCGCTGATGGCGATGAATGACCACTGTGGCAAGCCGCTTAATGCCACTCGTATGCTGGCTTTGATGGGCGAGCTgg                                                                                                                          >  1:128926/1‑250 (MQ=255)                                                                                                                       ccccggacgtctggCCGTTAAGCTGCCTGCAGAACCCCGGGAAACTATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCGTGGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAg                                                                                                                       <  2:158633/238‑1 (MQ=255)                                                                                                                          cGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTTGGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAggac                                                                                                                    <  1:302608/251‑3 (MQ=255)                                                                                                                            gACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTTGGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAggacg                                                                                                                   >  1:348065/1‑247 (MQ=255)                                                                                                                                  ttGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTTGGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTGCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAAtct                                                                                                              <  1:215774/249‑1 (MQ=255)                                                                                                                                    gCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTTGGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGCAGGACGAATCTCCgg                                                                                                          >  1:147606/1‑251 (MQ=255)                                                                                                                                        aTAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTTGGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGgcagc                                                                                                      >  1:411007/1‑251 (MQ=255)                                                                                                                                          atgCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTTGGCAAGCAAATTCCAGTGGTGATGATTCTGGAAAAGAATATGCCAATCGGTTCGGTCTTAGTCTCCAGCGCCCGTTCTGTGGTCGCGGCGCTGATGGCGTTGAATGAACGCTGTGGTAAGCCGCTTAATGACACTCGTTTGCTGGCTTGGGTTGGCGAGCTGGAAGGACGAATCTCCGgcagaat                                                                                                    <  1:23326/249‑4 (MQ=255)                                                                                                                                            gCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTTGGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGcattc                                                                                                  >  2:406777/1‑251 (MQ=255)                                                                                                                                                 cGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTTGGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTAc                                                                                             <  2:272209/251‑1 (MQ=255)                                                                                                                                                      gAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGACAGGAGCTTGGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGCTGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACa                                                                                         >  2:46101/1‑250 (MQ=255)                                                                                                                                                                     atCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTTGGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGttt                                                                         <  1:424535/251‑1 (MQ=255)                                                                                                                                                                       cGTTTATCAGTGCTGGGGGCGTGTGTGCCAGGAGCTTGGCAAGCAAATTCCAGTGGCGATGACGCGGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGGTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGTCACCGTGtttt                                                                        <  2:3633/250‑1 (MQ=255)                                                                                                                                                                             tCAGTGCTGGGAGCGTTTTTGCCAGGAGCTTGGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTggtg                                                                 >  1:195949/1‑251 (MQ=255)                                                                                                                                                                                            tttttGCCAGGAGCTTGGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGCCACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGAACCGTGTTTTCTTGGTGGTATTCAGTtgatg                                                   >  2:358672/1‑250 (MQ=255)                                                                                                                                                                                               ttgtccggagcttgggaaGCAAACTCCCGTGGCGATGACTCTTGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGACTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACTCTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTTCGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATc                                                <  2:119142/234‑1 (MQ=255)                                                                                                                                                                                               ttGCCAGGAGCTTGGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCAGTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATc                                                <  1:246690/250‑1 (MQ=255)                                                                                                                                                                                               ttGCCAGGAGCTGTGCAAGCAAATTCCAGTGGCGATGACTCGGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATc                                                <  2:429023/250‑1 (MQ=255)                                                                                                                                                                                                 gCCAGGAGCTTGGCAAGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCgaa                                             <  1:217992/251‑1 (MQ=255)                                                                                                                                                                                                    aggagcttggcaaGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACCCTGTGGCAAGCCGCTTACTGACAATCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACGACCTGGCCACGTGTTTTATTGGTGGTATGCAGTTGATGATCgaaga                                           >  2:192633/12‑250 (MQ=255)                                                                                                                                                                                                    aggagcttggcaaGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCCTTCATTACGACAACGTGGCCCCGTGTTTTCTTGGTGGTATGCAGTTGATGATCgaaga                                           >  2:243706/12‑250 (MQ=255)                                                                                                                                                                                                            ggcaaGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACTCTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAgg                                                                                                                      <  1:394805/164‑1 (MQ=255)                                                                                                                                                                                                            ggcaaGCAAATTCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTTATGGGCGAGCTGGAAgg                                                                                                                      >  2:394805/4‑167 (MQ=255)                                                                                                                                                                                                            ggcaaGCAAATGCCAGTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCTGTGGTCGAGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAATGACGAATCTCCGGCAGCATTCATTACGACAACGCGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAACACGCcat                                  <  2:154563/248‑1 (MQ=255)                                                                                                                                                                                                                        ccAGTGGCGATGACTCTGGAAAAGAATATGCCAAGCGGTTCGGGCTTAGGCTCCAGCGCCTGTGCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATcagccagc                       <  2:121006/250‑1 (MQ=255)                                                                                                                                                                                                                           gTGGCGATGACTCTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAgg                   >  1:81131/1‑250 (MQ=255)                                                                                                                                                                                                                               cgttGACTCTGGAAAAGAATATGCCCATCGGTTGGGGCATAGGCACCAGCACCTGTTCGACGGTCGCGGAGCTGACGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGGGCTGGATGGACGATTCTCCGGCAGCATTCATTACGACAACGAGGCACCGTGATTTCTTGGTGGTATGCAATCGCTGATCGAAGAAAACCACATCATCAGCCAGCAAGTggcg               >  2:174705/4‑248 (MQ=255)                                                                                                                                                                                                                                   gactcTGGAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCATTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAggg            <  1:143692/246‑1 (MQ=255)                                                                                                                                                                                                                                        tGGAAAAGAATATGGAAAGCGGTTAGGGATTAGGCTAAAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGACTGAACACTGTGGCAAGCCGCTTAATGAAACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTtgat      >  1:358998/1‑251 (MQ=255)                                                                                                                                                                                                                                         ggAAAAGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGGGCCAGGGTTtgatg     >  2:338302/1‑251 (MQ=255)                                                                                                                                                                                                                                            aaaGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGt  <  1:414371/251‑1 (MQ=255)                                                                                                                                                                                                                                            aaaGAATATGCCAATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGt  >  1:310392/1‑251 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                               CCAGTGCCAATATGAGCGTCGGGTTTGATGTGCTCGGGGCGGCGGTGACACCTGTTGATGGTGCATTGCTCGGAGATGTAGTCACGGTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAACTGGGTAAGCAAATTCCAGTGGCGATGACCCTGGAAAAGAATATGCCGATCGGTTCGGGCTTAGGCTCCAGTGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGCGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGCCGTATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTCGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGT  >  NC_000913/2826‑3311

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑