Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,623,838	G→A	Q265Q (CAG→CAA)	ppk →	polyphosphate kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,623,838	0	G	A	100.0%	146.4 / NA	47	Q265Q (CAG→CAA)	ppk	polyphosphate kinase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (18/29);  total (18/29)

TTACCGCCAGAAGCGCCGCGTCGACGCAAGCCGATGATTCTTCTGGATAACATTCTGCGTTACTGCCTTGATGATATTTTCAAAGGCTTCTTTGATTATGACGCGCTGAATGCCTATTCAATGAAGATGACCCGCGATGCCGAATACGATTTAGTGCATGAGATGGAAGCCAGCCTGATGGAGTTGATGTCTTCCAGTCTCAAGCAGCGTTTAACTGCTGAGCCGGTGCGTTTTGTTTATCAGCGCGATATGCCCAATGCGCTGGTTGAAGTGTTACGCGAAAAACTGACTATTTCCCGCTACGACTCCATCGTCCCCGGCGGTCGTTATCATAATTTTAAAGACTTTATTAATTTCCCCAATGTCGGCAAAGCCAATCTGGTGAACAAACCACTGCCGCGTTTACGCCATATTTGGTTTGATAAAGCCCAGTTCCGCAATGGTTTTGATGCCATTCGCGAACGCGATGTGTTGCTCTATT  >  NC_000913/2623596‑2624076                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                               ttACCGCCAGAAGCGCCGCGTCGACGCAAGCCGATGATTCTTCTGGATAACATTCTGCGTTACTGCCTTGATGATATTTTCAAAGGCTTCTTTGATTATGACGCGCTGAATGCCTATTCAATGAAGATGACCCGCGATGCCGAATACGATTTAGTGCATGAGATGGAAGCCAGCCTGATGGAGTTGATGTCTTCCAGTCTCAAGCAGCGTTTAACTGCTGAGCCGGTGCGTTTTGTTTATCAACGCGatat                                                                                                                                                                                                                                        >  1:464367/1‑251 (MQ=255)       ccaaaaGCGCCGCGTCGACTCAAGCCGATGATTCTTCTGGATAACATTCTGCGTTACTGCCTTGATGATATTTTCAAATGCTTCTTTGATTATGACGCGCTGAATGCCTATTCAATGAAGATGACCCGCGATGCCGAATACGATTTAGTTCATGAGATGGAAGCCAGCCTGATGGAGTTGATGTCTTCCAGTCTCAAGCAGCGTTTAACGGCTGAGCCTGTGCGTTTTGTTTATCAACGCGATATGccc                                                                                                                                                                                                                                    <  1:313885/245‑1 (MQ=255)         aGAAGCGCCGCGTCGACGCAAGCCGATGATTCTTCTGGATAACATTCTGCGTTACTGCCTTGATGATATTTTCAAAGGCTTCTTTGATTATGACGCGCTGAATGCCTATTCAATGAAGATGACCCGCGATGCCGAATACGATTTAGTGCATGAGATGGAAGCCAGCCTGATGGAGTTGATGTCTTCCAGTCTCAAGCAGCGTTTAACTGCTGAGCCGGTGCGTTTTGTTTATCAACGCGATATGCCCAATg                                                                                                                                                                                                                                <  1:315891/251‑1 (MQ=255)                       gACGCAAGCCTATGATTCTTCTGGATAACATTCGGCGTTACTGCCTTGATGATATTTTCAAAGGCTTCTTTGATTATGACGCGCTGAATGCCTATTCAATGAAGATGACCCGCGATGCCGAATACGATTTAGTGCATGAGATGGAAGCCAGCCTGATGGAGTTGATGTCTTCCAGTCTCAAGCAGCGTTTAACTGCTGAGCCGGTGCGTTTTGTTTATCAACGCGATATGCCCAATGCGCTGGTTGAAgtg                                                                                                                                                                                                                  <  1:77022/251‑1 (MQ=255)                           cAAGCCGATGATTCTTCTGGATAACATTCTGCGTTACTGCCTTGATGATATTTTCAAAGGCTTCTTTGATTATGACGCGCTGAATGCCTATTCAATGAAGATGACCCGCGATGCCGAATACGATTTAGTGCATGAGATGGAAGCCAGCCTGATGGAGTTGATGTCTTCCAGTCTCAAGCAGCGTTTAACTGCTGAGCCGGTGCGTTTTGTTTATCAACGCGATATGCCCAATGCGCTGGTTGAAGTGTTAc                                                                                                                                                                                                              <  1:376428/251‑1 (MQ=255)                           cAAGCCGATGATTCTTCTGGATAACATTCTGCGTTACTGCCTTGATGATATTTTCAAAGGCTTCTTTGATTATGACGCGCTGAATGCCTATTCAATGAAGATGACCCGCGATGCCGAATACGATTTAGTGCATGAGATGGAAGCCAGCCTGATGGAGTTGATGTCTTCCAGTCTCAAGCAGCGTTTAACTGCTGAGCCGGTGCGTTTTGTTTATCAACGCGATATGCCCAATGCGCTGGTTGAAGTGTTAc                                                                                                                                                                                                              <  1:489270/251‑1 (MQ=255)                                 gatgatTCTTCTGGATAACATTCCGCGTTACTGCCTTGATGATATTTTCAAAGGCTTCTTTGATTATGACGCGCTGAATGCCTATTCAATGAAGATGACCCGCGATGCCGAATACGATTTAGTGCATGAGATGGAAGCCAGCCTGATGGAGTTGATGTCTTCCAGTCTCAAGCAGCGTTTAACTGCTGAGCCGGTGCGTTTTGTTTATCAACGCGATATGCCCAATGCGCTGGTTGAAGTGTTACGCGaaa                                                                                                                                                                                                        <  1:1623/251‑1 (MQ=255)                                                   cATTCTGCGTTACTGCCTTGATGATATTTTCAAAGGCTTCTTTGATTATGACGCGCTGCATGCCTATTCAATGAAGATGACCCGCGATGCCGAATACGATTTAGTGCATGAGATGGAAGCCAGCCTGATGGAGTTGATGTCTTCCAGTCTCAAGCAGCGTTTAACTGCTGAGCCGGTGCGTTTTGTTTATCAACGCGATATGCCCAATGCGCTGGTTGAAGTGTTACGCGAAAAACTGACTATTTCCAGCt                                                                                                                                                                                      >  2:178406/1‑251 (MQ=255)                                                    aTTCTGCGTTACTGCCTTGATGATATTTTCAAAGGCTTCTTTGATTATGACGCGCTGAATGCCTATTCAATGAAGATGACCCGCGATGCCGAATACGATTTAGTGCATGAGATGGAAGCCAGCCTGATGGAGTTGATGTCTTCCAGTCTCAAGCAGCGTTTAACTGCTGAGCCGGTGCGTTTTGTTTATCAAAGCGATATGCCCAATGCGCTGGTTGAAGTGTTACGCGAAAAACTGACTATTTCCCGCt                                                                                                                                                                                      >  1:180284/1‑250 (MQ=255)                                                    aTTCTGCGTTACTGCCGTGATGATATTTTCAAAGGCTTCTGTGATTATGACGCGCTGAATGCCTATTCAATGAAGATGACCCGCGATGCCGAATACGATTTAGTGCATGAGAGGGAAGCCAGCCTGATGGAGTTGATGTCTTCCAGTCTCAAGCAGCGTTTAACGGCTGAGCCGGTGCGTGTTGTTTATCAACGCGATAGGCCCAATGCGCTGGTTGAAGTGTTACGCGAAAAACTGACTATTTCCCGCTa                                                                                                                                                                                     <  1:66716/251‑1 (MQ=255)                                                                 gCCTTGATGATATTTTCAAAGGCTTCTTTGCTTATGACGCGCTGAATGCCTATTCAATGAAGATGACCCGCGATGCCGACTACGATTTAGTGCATGAGATGGAAGCCAGCCTGATGGAGTTGATGTCTTCCAGTCTCAAGCAGCGTTTAACTGCTGAGCCGGTGCGTTTTGTTTATCAACGCGATATGCCCAATGCGCTGGTTGAAGTGTTACGCGAAAAACTGACTATTTCCCGATACGACTCCATCGTc                                                                                                                                                                        >  2:239976/1‑251 (MQ=255)                                                                     tgatgaTATTTTCAAAGGCTTCTTTGATTATGACGCGCTGAATGCCTATTCAATGAAGATGACCCGCGATGCCGAATACGATTTAGTGCATGAGATGGAAGCCAGCCTGATGGAGTTGATGTCTTCCAGTCTCAAGCAGCGTTTAACTGCTGAGCCGGTGCGTTTTGTTTATCAACGCGATATGCCCAATGCGCTGGTTGAAGGGTTCCGCGAAAAACTGACTATTTCCCGCTACGACTCCATCGTCCCcg                                                                                                                                                                    >  2:71341/1‑251 (MQ=255)                                                                          atatTTTCAAAGGCTTCTTTGATTATGACGCGCTGAATGCCTATTCAATGAAGATGACCCGCGATGCCGAATACGATTTAGTGCATGAGATGGAAGCCAGCCTGATGGAGTTGATGTCTTCCAGTCTCAAGCAGCGTTTAACTGCTGAGCCGGTGCGTTTTGTTTATCAACGCGATATGCCCAATGCGCTGGTTGAAGTGTTACGCGAAAAACTGACTATTTCCCGCTACGCCTCCATCGTCCCCGGCGGt                                                                                                                                                               <  1:239883/251‑1 (MQ=255)                                                                          atatTTTCAAAGGCTTCTTTGATTATGACGCGCTGAATGCCTATTCAATGAAGATGACCCGCGATGCCGAATACGATTTAGTGCATGAGATGGAAGCCAGCCTGATGGAGTTGATGTCTTCCAGTCTCAAGCAGCGTTTAACTGCTGAGCCGGTGCGTTTTGTTTATCAACGCGATATGCCCAATGCGCTGGTTGAAGTGTTACGCGAAAAACTGACTATTTCCCGCTACGACTCCATCGTCCCCGGCGGt                                                                                                                                                               <  1:432475/251‑1 (MQ=255)                                                                            atTTTCAAAGGCTTCTTTGATTATGACGCGCTGAATGCCTATTCAATGAAGATGACCCGCGATGCCGAATACGATTTAGTGCATGAGATGGAAGCCAGCCTGATGGAGTTGATGTCTTCCAGTCTCAAGCAGCGTTTAACTGCTGAGCCGGTGCGTTTTGTTTATCAACGCGATATGCCCAATGCGCTGGTTGAAGTGTTACGCGAAAAACTGACTATGTCCCGCTACGACTCCATCGTCCCCGGCGGTCg                                                                                                                                                             <  1:254730/251‑1 (MQ=255)                                                                                 cAAAGGCTTCTTTGATTATGACGCGCTGAATGCCTATTCAATGAAGATGACCCGCGATGCAGAATACGATTTAGTGCATGAGATGGAAGCCAGCCTGATGGAGTTGCTGTCTTCCAGTCTCAAGCAGCGTTTAACTGCTGAGCCGGTGCGTTTTGTTTATCAACGCGATATGCCCAATGCGCTGGTTGCAGTGTTACGCGAAAAACTGACTATTTCCCGCTACGACTCCATCGTCCCCGGCGGTCGTTATc                                                                                                                                                        >  1:301035/1‑251 (MQ=255)                                                                                     ggCTTCTTTGATTATGACGCGCTGAATGCCTATTCAATGAAGATGACCCGCGATGCCGAATACGATTTAGTGCATGAGATGGAAGCCAGCCTGATGGAGTTGATGTCTTCCAGTCTCAAGCAGCGTTTAACTGCTGAGCCGGTGCGTTTTGTTTATCAACGCGATATGCCCAATGCGCTGGTTGAAGTGTTACGCGAAAAACTGACTATTTCCCGCTACGACTCCATCGTCCCCGGCGGTCGTTATCATa                                                                                                                                                     >  1:212481/1‑250 (MQ=255)                                                                                      gCTTCTTTGATTATGACGCGCTGAATGCCTATTCAATGAAGATGACCCGCGATGCCGAATACGATTTAGTGCATGAGATGGAAGCCAGCCTGATGGAGTTGATGTCTTCCAGTCTCAAGCAGCGTTTAACTGCTGAGCCGGTGCGTTTTGTTTATCAACGCGATATGCCCAATGCGCTGGTTGAAGTGTTACGCGAAAAACTGACTATTTCCCGCTACGACTCCATCGTCCCCGGCGGTCGTTATCATAAt                                                                                                                                                   <  1:90556/251‑1 (MQ=255)                                                                                                      cgcgCTGAATGCCTATTCAATGAAGATGACCCGCGATGCCGAATACGATTTAGTGCATGAGATGGAAGCCAGCCTGATGGAGTTGATGTCTTCCAGTCTCAAGCAGCGTTTAACTGCTGAGCCGGTGCGTTTTGTTTATCAACGCGATATGCCCAATGCGCTGGTTGAAGTGTTACGCGAAAAACTGACTATTTCCCGCTACGACTCCATCGTCCCCGGCGGTCGTTATCATAATTTTAAAGACTttatta                                                                                                                                   <  1:447714/251‑1 (MQ=255)                                                                                                              aTGCCTATTCAATGAAGATGACCCGCGATGCCGAATACGATTTAGTGCATGAGATGGAAGCCAGCCTGATGGAGTTGATGTCTTCCAGTCTCAAGCAGCGTTTAACTGCTGAGCCGGTGCGTTTTGTTTATCAACGCGATATGCCCAATGCGCTGGTTGAAGTGTTACGCGAAAAACTGACTATTTCCCGCTACGACTCCATCGTCCCCGGCGGTCGTTATCATAATTTTAAAGACTTTATTAATTTcccc                                                                                                                           <  1:462489/251‑1 (MQ=255)                                                                                                                   tATTCAATGAAGATGACCCGCGATGCCGAATACGATTTAGTGCATGAGATGGAAGCCAGCCTGATGGAGTTGATGTCTTCCAGTCTCAAGCAGCGTTTAACTGCTGAGCCGGTGCGTTTTGTTTATCAACGCGATATGCCCAATGCGCTg                                                                                                                                                                                                                           >  1:184337/1‑150 (MQ=255)                                                                                                                   tATTCAATGAAGATGACCCGCGATGCCGAATACGATTTAGTGCATGAGATGGAAGCCAGCCTGATGGAGTTGATGTCTTCCAGTCTCAAGCAGCGTTTAACTGCTGAGCCGGTGCGTTTTGTTTATCAACGCGATATGCCCAATGCGCTg                                                                                                                                                                                                                           <  2:184337/150‑1 (MQ=255)                                                                                                                               aTGACCCGCGATGCCGAATACGATTTAGTGCATGAGATGGAAGCCAGCCTGATGGAGTTGATGTCTTCCAGTCTCAAGCAGCGTTTAACTGCTGAGCCGGTGCGTTTTGTTTATCAACGCGATATGCCCAATGCGCTGGTTGAAGTGTTACGCGAAAAACTGACTATTTCCCGCTACGACTCCATCGTCCCCGGCGGTCGTTATCATAATTTTAAAGACTTTATTAATTTCCCCAATGTCGGCAAAGCCa                                                                                                           >  1:187956/1‑250 (MQ=255)                                                                                                                                           gCCGAATACGATTTAGTGCATGAGATGGAAGCCAGCCTGATGGAGTTGATGTCTTCCAGTCTCAAGCAGCGTTTAACTGCTGAGCCGGTGCGTTTTGTTTATCAACGCGATATGCCCAATGCGCTGGTTGAAGTGTTACGCGAAAAACTGACTATTTCCCGCTACGACTCCATCGTCCCCGGCGGTCGTTATCATAATTTTAAAGACTTTATTAATTTCCCCAATGTCGGCAAAGCCAATCTGGTGAACa                                                                                               >  1:265497/1‑250 (MQ=255)                                                                                                                                                          gTGCATGAGATGGAAGCCAGCTTGATGGAATGTATGTCTTCCAGTCTCAAGCAGCGTTTAACTGCTGAGCCGGTGCGTTTTGTTTATCAACGCGATATGCCCAATGCGCTGGTTGAAGTGTTACGCGAAAAACTGACTATTTCCCGATACGACTCCATCGTCCCCGGCGGTCGTTATCATAATTTTAAAGACTTTATTAATTTCCCCAATGTCGGCAAAGCCAATCTGGTGAACAAACCACTGCCGCGttt                                                                               <  1:65596/251‑1 (MQ=255)                                                                                                                                                            gCATGAGATGGAAGCCAGCCTGATGGAGTTGATGTCTTCCAGTCTCAAGCAGCGTTTAACTGCTGAGCCGGTGCGTTTTGTTTATCAACGCGATATGCCCAATGCGCTGGTTGAAGTGTTACGCGAAAAACTGACTATTTCCCGCTACGACTCCATCGTCCCCGGCGGTCGTTATCATAATTTTAAAGACTTTATTAATTTCCCCAATGTCGGCAAAGCCAATCTGGTGAACAAACCCCTGCCGCGTTTAc                                                                             <  1:425912/251‑1 (MQ=255)                                                                                                                                                              aggagaTGGAAGCCAGCCTGATGGAGTTGATGTCTTCCAGTCTCAAGCAGCGTTTAACTGCTGAGCCGGTGCGTTTTGTTTATCAACGCGATATGCCCAATGCGCTGGTTGAAGTGTTACGCGAAAAACTGACTATTTCCCGCTACGACTCCATCGTCCCCGGCGGTCGTTATCATAATTTTAAAGACTTTATTAAtt                                                                                                                                <  1:227474/196‑1 (MQ=255)                                                                                                                                                              aTGAGATGGAAGCCAGCCTGATGGAGTTGATGTCTTCCAGTCTCAAGCAGCGTTTAACTGCTGAGCCGGTGCGTTTTGTTTATCAACGCGATATGCCCAATGCGCTGGTTGAAGAGTTACGCGAAAAACTGACTATTTCCCGCTACGACTCCATCGTCCCCGGCGGGCGTTATCATAATTTTAAAGCCTTTATTAAtt                                                                                                                                >  2:227474/1‑198 (MQ=255)                                                                                                                                                               tGAGATGGAAGCCAGCCTGATGGAGTTGATGTCTTCCAGTCTCAAGCAGCGTTTAACTGCTGAGCCGGTGCGTTTTGTTTATCAACGCGATATGCCCAATGCGCTGGTTGAAGTGTTACGCGAAAAACTGACTATTTCCCGCTACGACTCCATCGTCCCCGGCGGTCGTTATCATAATTTTAAAGACTTTATTAATTTCCCCAATGTCGGCAAAGCCACTCTGATGACCAAACCACTGCCGCGTTTAcacc                                                                          >  2:347497/1‑248 (MQ=255)                                                                                                                                                                   aTGGAAGCCAGCCTGATGGAGTTGATGTCTTCCAGTCGCAAGCAGCGTTTAACTGCTGAGCCGGTGCGTTTTGTTTATCAACGCGATATGCCCAATGCGCTGGTGGAAGTGTTACGCGAAAAACTGACTATTTCCCGCTACGACTCCATCGTCCCCGGCGGTCGTTATCATAATTTTAAAGACTTTATTAATTTCCCCAATGTCGGCAAAGCCAATCTGGTGAACAAACCACTGCCGCGTTTACGCCatat                                                                      <  2:225994/251‑1 (MQ=255)                                                                                                                                                                     ggAAGCCAGCCTGATGGAGTTGATGTCTTCCAGTCTCAAGCAGCGTTTAACTGCTGAGCCGGTGCGTTTTGTTTATCAACGCGATATGCCCAATGCGCTGGTTGAAGTGTTACGCGAAAAACTGACTATTTCCCGCTACGACTCCATCGTCCCCGGCGGTCGTTATCATAATTTTAAAGACTTTATTAATTTCCCCAATGTCGGCAAAGCCAATCTGGTGAACAAACCACTGCCGCGTTTACGCCATAttt                                                                    <  1:284674/251‑1 (MQ=255)                                                                                                                                                                     ggAAGCCAGCCTGATGGAGTTGATGTCTTCCAGACTCAAGCAGCGTTTAACTGCTGAGCCGGTGCGTTTTGTTTATCAACGCGATATGCCCAACGCGCTGGTTGAAGTGTTACGCGAAAAACTGACTATTTCCCGCTACGACTCCATCGTCCCCGGCGGTCGTTATCATAATTTTAAAGACTTTATTAATTTCCCCAATGTCGGCAAAGCCAATCTGGTGAACAAACCACTGCCGCGTTTACGCCATAttt                                                                    <  1:340612/251‑1 (MQ=255)                                                                                                                                                                        agccagccTGATGGAGTTGATGTCTTCCAGTCTCAAGCAGCGTTTAACTGCTGAGCCGGTGCGTTTTGTTTATCAACGCGATATGCCCAATGCGCTGGTTGAAGTGTTACGCGAAAAACTGACTATTTCCCGCTACGACTCCATCGTCCCCGGCGGTCGTTATCATAATTTTAAAGACTTTATTAATTTCCCCAATGTCGGCAAAGCCAATCTGGTGAACAAACCACTGCCGCGTTTACGCCATATTTGGt                                                                 <  1:489325/251‑1 (MQ=255)                                                                                                                                                                           cagccTGGTGGATTTGATGTCTTCCAGTCTCAAGCAGCGTTTAACTGCTGTGCCGGTGCGTTTTGTTTATCAACGCGATATGCCCAATGCGCTGGTTGAAGTGTTACGCGAAAAACTGCCTATTTCCCGCTACGACTCCATCGTCCCCGGCGGTCGTTATCATAATTTTAAAGACTTTATTAATTTCCCCAATGTCGGCAAAGCCAATCTGGTGAACAAACCACTGCAGCGGTTACGCCATATCGGGTTTg                                                              <  2:100076/251‑1 (MQ=255)                                                                                                                                                                            agccTGATGGAGTTGATGTCTTCCAGTCTCAAGCAGCGTTTAACTGCTGAGCCGGTGCGTTTTGTTTATCAACGCGATATGCCCAATGCGCTGGTTGAAGTGTTACGCGAAAAACTGACTATTTCCCGCTACGACTCCATCGTCCCCGGCGGTCGTTATCATAATTTTAAAGACTTTATTAATTTCCCCAATGTCGGCAAAGCCAATCTTGTGAACAAACAACTGCCGCGTTTACGCCATATTTGGTTTg                                                              >  1:183344/1‑250 (MQ=255)                                                                                                                                                                              ccTGATGGAGTTGATGTCTTCCAGTCTCAAGCAGCGTTTAACTGCTGAGCCGGTGCGTTTTGTTTATCAACGCGATATGCCCAATGCGCTGGTTGAAGTGTTACGCGAAAAACTGACTATTTCCCGCTACGACTCCATCGTCCCCGGCGGTCGTTATCATAATTTTAAAGACTTTATTAATTTCCCCAATGTCGGCAAAGCCAATCTGGTGAACAAACCACTGCCGCGTTTACGCCATATTTGGTTTGATa                                                           <  1:430100/251‑1 (MQ=255)                                                                                                                                                                                      ggTTGATGTCTTCCAGTCTCAAGCAGCGTTTAACTGCTGAGCCGGTGCGTTTTGTTTATCAACGCGATATGCCCAATGCGCTGGTTGAAGTGTTACGCGAAAAACTGACTATTTCCCGCTACGACTCCATCGTCCCCGGCGGTCGTTATCATAATTTTAAAGACTTTATTAATTTCCCCAATGTCGGCAAAGCCAATCTGGTGAACAAACCACTGCCGCGTTTACGCCATATTTGGTTTGATAAAGCCCAg                                                   <  2:446108/250‑1 (MQ=255)                                                                                                                                                                                          gATGTCTTCCAGTCTCAAGCAGCGTTTAACTGCTGAGCCGGTGCGTTTTGTTTATCAACGCGATATGCCCAATGCGCTGGTTGAAGTGTTACGCGAAAAACTGACTATTTCCCGCTACGACTCCATCGTCCCCGGCGGTCGTTATCATAATTTTAAAGACTTTATTAATTTCCCCAATGTCGGCAAAGCCAATCTGGTGAACAAACCACTGCCGCGTTTACGCCATATTTGGTTTGATAAAGCCCAGTTcc                                               >  1:377835/1‑251 (MQ=255)                                                                                                                                                                                                          aaGCAGCGTTTAACTGCTGAGCCGGTGCGTTTTGTTTATCAACGCGATATGCCCAATGCGCTGGTTGAAGTGTTACGCGAAAAACTGACTATTTCCCGCTACGACTCCATCGTCCCCGGCGGTCGTTATCATAATTTTAAAGACTTTATTAATTTCCCCAATGTCGGCAAAGCCAATCTGGTGAACAAACCACTGCCGCGTTTACGCCATATTTGGTTTGATAAAGCCCAGTTCCGCAATGGTTTTGATGc                               <  1:490299/251‑1 (MQ=255)                                                                                                                                                                                                              agcGTTTAACTGCTGAGCCGGTGCGTTTTGTTTAACAACGCGATATGCCCAATGCGCTGGTTGAAGTGTTACGCGAAAAACTGACTATTTCCCGCTACGACTCCATCGTCCCCGGCGGTCGTTATCATAATTTTAAAGACTTTATTAATTTCCCCAATGTCGGCAAAGCCAATCTGGTGAACAAACCACTGCCGCGTTTACGCCATATTTGGTTTGATAAAGCCCAGTTCCGCAATGGTTTTGATGCCAtt                           <  2:377835/251‑1 (MQ=255)                                                                                                                                                                                                                 gTTTAACTGCTGAGCCGGTGCGTTTTGTTTATCAACGCGATATGCCCAATGCGCTGGTTGAAGTGTTACGCGAAAAACTGACTATTTCCCGCTACGACTCCATCGTCCCCGGCGGTCGTTCTCATAATTTTAAAGACTTTATTAATTTCCCCAATGTCGGCAAAGCCAATCTGGTGAACAAACCACTGCCGCGTTTACGCCATATTTGGTTTGATAAAGCCCAGTTCCGCAATGGTTTTGCTGCCATTcgc                        >  1:127518/1‑251 (MQ=255)                                                                                                                                                                                                                     aaCTGCTGATCCGGTGCGTTTTGTTTATCAACGCGATATGCCCAATGCGCTGGTTGAAGTGTTACGCGAAAAACTGACTATTTCCCGCTACGACTCCATCGTCCCCGGCGGTCGTTATCATAATTTTAAAGACTTTATTAATTTCCCCAATGTCGGCAAAGCCAATCTGGTGAACAAACCACTGCCGCGTTTACGCCATATTTGGTTTGATAAAGCCCAGTTCCGCAATGGTTTTGATGCCATTCGCGAAc                    <  1:275851/251‑1 (MQ=255)                                                                                                                                                                                                                      actgcttagccgtggggTTTTGTTTAACAACGCGATATTCCCAATGCGCTGGTTGAAGTGTTACGCGAAAAACTGACTATTTCCCGCTACGACTCCATCGTCCCCGGCGGTCGTTATCATAATTTTAAAGACTTTATTAATTTCCCCAATGTCGGCAAAGCCAATCTGGTGAACAAACCACTGCCGCGTTTACGCCATATTTGTTTTGATAAAGCCCAGTTCCGCAATGGTTTTTATGCCATTCGCGAAcg                   <  2:425762/235‑1 (MQ=255)                                                                                                                                                                                                                           tgAGCCGGTGCGTTTTGTTTATCAACGCGATATGCCCAATGCGCTGGTTGAAGTGTTACGCGAAAAACTGACTATTTCCCGCTACGACTCCATCGTCCCCGGCGGTCGTTATCATAATTTTAAAGACTTTATTAATTTCCCCAATGTCGGCAAAGCCAATCTGGTGAACAAACCACTGCCGCGTTTACGCCATATTTGGTTTGATAAAGCCCAGTTCCGCAATGGTTTTGATGCCATTCGCGAACGCGAtg              >  1:491687/1‑251 (MQ=255)                                                                                                                                                                                                                            gAGCCGGTGCGTTTTGTTTATCAACGCGATATGCCCAATGCGCTGGTTGAAGTGTTACGCGAAAAACTGACTATTTCCCGCTACGACTCCATCGTCCCCGGCGGTCGTTATCATAATTTTAAAGACTTTATTAATTTCCCCAATGTCGGCAAAGCCAATCTGGAGAACAAACCACTGCCGCGTTTACGCCATATTTGGTTTGATAAAGCCCAGTTCCGCAATGGTTTTGATGCCATTCGCGAACGCGAtgg             >  2:100519/1‑250 (MQ=255)                                                                                                                                                                                                                                    gctgtttGTTTATCAACGCGATATGCCCAATGCGCTGGTTGAAGTGTTACGCGAAAAACTGACTATTTCCCGCTACGACTCCATCGTCCCCGGCGGTCGTTATCATAATTTTAAAGACTTTATTAATTTCCCCAATGTCGGCAAAGCCAATCTGGTGAACAAACCACTGCCGCGTTTACGCCATATTTGGTTTGATAAAGCCCAGTTCCGCAATGGTTTTGATGCCATTCGCGAACGCGATGTGTTGctct     <  2:464367/247‑1 (MQ=255)                                                                                                                                                                                                                                        tttGTTTATCAACGCGATATGCCCCATGCGCTGGTTGCAGTGTTACGCGAAAAACTGACTATTTCCCGCTACGACTCCATCGTCCCCGGCGGTCGATAACATAATTTTAAAGCCTTTATTAATTTCCCCAATGTCGGCAAAGCCAATCTGGTGCACAAACCACTGCCGCGTTTCCGCCATATTTGGTTTTATATAGACCAGATCCGCACTGGTTTTGATGACACTCTCAACCGCGATGTGTTGCTCtatt  >  2:88024/1‑250 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                               TTACCGCCAGAAGCGCCGCGTCGACGCAAGCCGATGATTCTTCTGGATAACATTCTGCGTTACTGCCTTGATGATATTTTCAAAGGCTTCTTTGATTATGACGCGCTGAATGCCTATTCAATGAAGATGACCCGCGATGCCGAATACGATTTAGTGCATGAGATGGAAGCCAGCCTGATGGAGTTGATGTCTTCCAGTCTCAAGCAGCGTTTAACTGCTGAGCCGGTGCGTTTTGTTTATCAGCGCGATATGCCCAATGCGCTGGTTGAAGTGTTACGCGAAAAACTGACTATTTCCCGCTACGACTCCATCGTCCCCGGCGGTCGTTATCATAATTTTAAAGACTTTATTAATTTCCCCAATGTCGGCAAAGCCAATCTGGTGAACAAACCACTGCCGCGTTTACGCCATATTTGGTTTGATAAAGCCCAGTTCCGCAATGGTTTTGATGCCATTCGCGAACGCGATGTGTTGCTCTATT  >  NC_000913/2623596‑2624076

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑