Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,259,422	A→C	intergenic (‑126/+297)	psuK ← / ← fruA	putative pseudouridine kinase/fructose‑specific PTS multiphosphoryl transfer protein FruA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,259,422	0	A	C	96.4%	86.3 / ‑5.2	28	intergenic (‑126/+297)	psuK/fruA	putative pseudouridine kinase/fructose‑specific PTS multiphosphoryl transfer protein FruA
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base C (18/9);  total (19/9)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.59e-01

CCAGGCGTAAATTTTATTTTACCTGGATTTGAATCCGCATAATTTAATGATTCATGTGAATATCCGGCGACATCAATATTCGCCGAACCTATAATTACGACATAATCCTTTTCGCGCATAGCACGTCCTCTGGTAAAAAATTACCAACCCTGTCAATCCACAAGAATATAGATACTGCAAAGACGGTTTAAACATGTGTTCATATTTAAACATGTGCTCATAGTATGCATGTTTTACGTAAAGTAAATTACCGCCAGAGAGGCAAAATGGGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTC  >  NC_000913/2259178‑2259661                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                ccaGGCGTAAATTTTATTTTACCTGGATTTGAATCCGCATAATTTAATGATTCATGTGAATATCCGGCGACATCAATATTCGCCGAACCTATAATTACGACATAATCCTTTTCGCGCATAGCACGTCCTCTGGTAAAAAATTACCAACCCTGTCAATCCACAAGAATATAGATACTGCAAAGACGGTTTAAACATGTGTTCATATTTAAACATGTGCTCATAGTATGCATGTTTTACGTAAAGTAAATTAc                                                                                                                                                                                                                                           >  2:4230/1‑251 (MQ=255)                     aCCTGGATTTGAATCCGCATAATTTAATGATTCATGTGAATATCCGGCGACATCAATATTCGCCGAACCTATAATTACGACATAATCCTTTTCGCGCATAGCACGTCCTCTGGTAAAAAATTACCAACCCTGTCAATCCACAAGAATATAGATACTGCAAAGACGGTTTAAACATGTGTTCATATTTAAACATGTGCTCATAGTATGCATGTTTTACGTAAAGTCAATTACCGCCAGAGAGGCAAAATggg                                                                                                                                                                                                                       >  1:352150/1‑251 (MQ=255)                               gAATCCGAATAATTTAATGATTCATGTGAATACCCGGCGACATCAATATTCGCCGAACCTATAATTACGACATAATCCTTTTCGCGCATAGCACGTCCTCTGGTAAAAAATTACCAACCCTGTCAATCCACAAGAATATAGATACTGCAAAGACGGTTTAAACATGTGTTCATATTTAAACATGTGCTCATAGTATGCATGTTTTACGTAAAGTCAATTACCGCCAGAGAGGCAAAATGGGCAGATTTGa                                                                                                                                                                                                              <  2:154295/250‑1 (MQ=255)                                     gCATAATGTAATGATTCATGTGAATATCCGGCGGCATCAATATTCGCCGAACCTATAATAACGACATAATCCTTTTCGCGCATAGCACGTCCTCTGGTAAAAAATTACCAACCCTGTCAATCCACAAGAATATAGATACTGCAAAGACGGTTTAAACATGTGTTCATATTTAAACATGTGCTCATAGTATGCATGTTTTACGTAAAGTCAATTACCGCCAGAGAGGCAAAATGGGCAGATTTGAGCAGGCt                                                                                                                                                                                                       <  2:73202/251‑1 (MQ=255)                                      cATAATTTAATGATTCATGTGAATATCCGGCGACATCAATATTCGCCGAACCTATAATTACGACATAATCCTTTTCGCGCATAGCACGTCCTCTGGTAAAAAATTACCAACCCTGTCAATCCACAAGAATATAGATACTGCAAAGACGGTTTAAACATGTGTTCATATTTAAACATGTGCTCATAGTATGCATGTTTTACGTAAAGTCAATTACCGCCAGAGAGGCAAAATGGGCAGATTTGAGCAGGCTc                                                                                                                                                                                                      >  1:387240/1‑251 (MQ=255)                                      cATAATTTAATGATTCATGTGAATATCCGGCGACATCAATATTCGCCGAACCTATAATTACGACATAATCCTTTTCGCGCATAGCACGTCCTCTGGTAAAAAATTACCAACCCTGTCAATCCACAAGAATATAGATACTGCAAAGACGGTTTAAACATGTGTTCATATTTAAACATGTGCTCATAGAATGCATGTTTTACGTAAAGTCAATTACCGCCAGAGAGGCAAAATGGGCAGATTTGAGCAGGCTc                                                                                                                                                                                                      >  2:422780/1‑251 (MQ=255)                                             tAATGATTCATGTGAATATCCGGCGACATCAATATTCGCCGAACCTATAATTACGACATAATCCTTTTCGCGCATAGCACGTCCTCTGGTAAAAAATTACCAACCCTGTCAATCCACAAGAATATAGATACTGCAAAGACGGTTTAAACATGTGTTCATATTTAAACATGTGCTCATAGTATGCATGTTTTACGTAAAGTCAATTACCGCCAGAGAGGCAAAATGGGCAGATTTGAGCAGGCTCACAAAAt                                                                                                                                                                                               >  1:16332/1‑251 (MQ=255)                                                             tatCCGGCGACATCAATATTCGCCGAACCTATAATTACGACATAATCCTTTTCGCGCATAGCACGTCCTCTGGTAAAAAATTACCAACCCTGTCAATCCACAAGAATATAGATACTGCAAAGACGGTTTAAACATGTGTTCATATTTAAACATGTGCTCATAGTATGCATGTTTTACGTAAAGTCAATTACCGCCAGAGAGGCAAAATGGGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTg                                                                                                                                                                                >  2:11909/1‑250 (MQ=255)                                                                          cAATATTCGCCGAACCTATAATTACGACAAAATCCTTTTCGCGAATAGCACGTCATCTGGTCAATACTTACCAACCCTGTCAATCCAGAAGAATATAGATACTGCAAAGCCGGTTTAAAAATGTGTTCATATTTAAACATGTGCTAATAGCATGCATGTCTTAAGTCAAGTCAATTACCGCCAGAGAGGCAAATAGGGCTGATTTGAACAGGATCACAAAATGCAATTAAATACCTAACTTACaaaatcg                                                                                                                                                                   >  2:167251/1‑247 (MQ=255)                                                                              atTCGCCGCACCTATAATTACGACATAATCCTTTTCGCGCATAGCACGTCCTCTGGTAAAAAATTACCAACCCTGTCAATCCACAAGAATATAGATACTGCAAAGACGGTTTAAACATGTGTTCATATTTAAACATGTGCTCATAGTATGCATGTTTTACGTAAAGTCAATTACCGCCAGAGAGGCAAAATGGGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGa                                                                                                                                                               >  2:473958/1‑250 (MQ=255)                                                                                 cGCCGAACCTATAATTACGACCTACTCCTTTTCGCGCATAGCACGTCCTCTGGTAAAAAATTACCAACCCTGTCAATCCACAAGAATATAGCTACTGCAAAGACGGTTTAAACATGTGTTCATATTTAAACATGTGCTCATAGTATGCATGTTTTACGTAAAGTCAATTACCGCCAGAGAGGCAAAATGGGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGACTGAATTACAAAAACGAGGCAttt                                                                                                                                                           >  2:249013/1‑251 (MQ=255)                                                                                        ccTATAATTACGACATAATCCTTTTCGCGCATAGCACGTCCTCTGGTAAAAAATTACCAACCCTGTCAATCCACAAGAATATAGATACTGCAAAGACGGTTTAAACATGTGTTCATATTTAAACATGTGCTCATAGTATGCATGTTTTACGTAAAGTCAATTACCGCCAGAGAGGCAAAATGGGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACgg                                                                                                                                                    >  1:274746/1‑251 (MQ=255)                                                                                                        aaTCCTTTTCGCGCATAGCACGTCCTCTGGTAAAAAATTACCAACCCTTTCAATCCACAAGAATAGAGATACTGCAAAGACGGTTTAAACATGTGTTCATATTTAAACATGTGCTCATAGTATGCATGTTTTACGTAAAGTCAATTACCGCCAGAGAGGCAAAATGGGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCAcagaacagaa                                                                                                                                    <  2:378680/251‑1 (MQ=255)                                                                                                                 cgcgCATAGCACGTCCTCTGGTAAAAAATTACCAACCCTGTCAATCCACAAGAATATAGATACTGCAAAGACGGTTTAAACATGTGTTCATATTTAAACATGTGCTCATAGTATGCATGTTTTACGTAAAGTCAATTACCGCCAGAGAGGCAAAATGGGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATaaaaa                                                                                                                           <  1:130178/251‑1 (MQ=255)                                                                                                                        aGCACGTCATCTGGTAAAAAATTACCAACCCTGTCAATCCACAAGAATATAGATACTGCAAAGACGGTTTAAACATGTGTTCATATTTAAACATGTGCTCATAGTATGCATGTTTTACGTAAAGTCAATTACCGCCAGAGAGGCAAAATGGGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGACTTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGc                                                                                                                      >  2:255787/1‑249 (MQ=255)                                                                                                                         gCACGTCCTCTGGTAAAAAATTACCAACCCTGTCAATCCACAAGAATATAGATACTGCAAAGACGGTTTAAACATGTGTTCATATTTAAACATGTGCTCATAGTATGCATGTTTTACGTAAAGTCAATTACCGCCAGAGAGGCAAAATGGGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGa                                                                                                                   <  1:11909/251‑1 (MQ=255)                                                                                                                               ccTCTGGTAAAAAATTACCAACCCTGTCAATCCACAAGAATATAGATACTGCAAAGACGGTTTAAACATGTGTTCATATTTAAACATGTGCTCATAGTATGCATGTTTTACGTAAAGTCAATTACCGCCAGAGAGGCAAAATGGGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAaata                                                                                                              <  1:361723/250‑1 (MQ=255)                                                                                                                                   tGGTAAAAAATTACCAACCCTGTCAATCCACAAGAATATAGATACTGCAAAGACGGTTTAAACATGAGTTCATATTTAAACATGTGCTCATAGTATGCATGTTTTACGTAAAGTCAATTACCGCCAGAGAGGCAAAATGGGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCAAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTg                                                                                                         >  1:262060/1‑251 (MQ=255)                                                                                                                                                                         aataTACTGCAAAGACGGTTTAAACATGTGTTCATATTTAAACATGTGCTCATAGTATGCATGTTTTACGTAAAGTCAATTACCGCCAGAGAGGCAAAATGGGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTg                                                                   <  2:16332/248‑1 (MQ=255)                                                                                                                                                                                tGCAAAGACGGTTTAACCATGTGTTCATAGTTAAGCAGGTGCTCATAGTATGCATTTTTTACGTAAAGTCAATTAACGCCAGAGGTACAAAATGGACAGATTTGAGAAGGCCCACAACCTCCGATTAAATGCCTTGATTACAAAAGCGAGGAATTTGTGACGGGATGAACAGAAACGAACAATACAAAAGGGCCGACAATACTCTGCACTTGTGGAAATAACCTGAGTGATtgatgacagattaacgttac                                                            >  2:216441/1‑237 (MQ=255)                                                                                                                                                                                 gaaaaGACGGTTTAAACATGTGTTCATATTTAAACATGAGCTCATAGTATGCATGTTTTACGTAAAGTCAATTACCGACAGAGAGGCAAAATGGGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCt                                                           >  1:108746/3‑251 (MQ=255)                                                                                                                                                                                          gTTTAAACATGTGTTCATATTTAAACATGTGCTCATAGAATGCATGTTTTACGTAAAGTCAATTACCGCCAGAGAGGCAAAATGGGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGaa                                                     >  1:191901/1‑248 (MQ=255)                                                                                                                                                                                            ttAAACATGTGTTCAAATTTAAACATGTGCTCATAGTATGCATGTTTTACGTAAAGTCAATTACCGCCAGAGAGGCAAAATGGGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTTCCCTTGAGGAAATAACCTGAGTTTTTGATGAACGTTTGAAGTTTCTACTGAAAGAt                                                 <  2:501430/250‑1 (MQ=255)                                                                                                                                                                                                           tatTTAAACATGTGCTCATAGTATGCATGTTTTACGTAAAGTCAATTACCGCCAGAGAGGCAAAATGGGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTc                                 >  2:80520/1‑251 (MQ=255)                                                                                                                                                                                                            atTTAAACATGAGCTCAGAGTATGCATGTTTTACGTAAAGTCAATTACCGCCAGAGAGGCAAAATGGGCAGATTTAAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGCATTTGTGACGGGATGCACAGAACAGAACACTAAAAAAGGGCAGAAAATAATCTGCACTTGAGGAAATAACCTGAATGTTTGATGAACGTTTGAAGTTTCTAATGAAAGATAAAACCATTTCAATTc                                 >  2:25408/1‑250 (MQ=255)                                                                                                                                                                                                                aaaCACGTGCTCATAGTATGCATGTTTTACGTAAAGTCAATTACCGCCAGAGCGCCAAAAAGGGCAGATTTGAGCATGCTCACAAACTGCGATTAACTGCCTGAATTACAAACGCGAGGAATTTGTGACGGCATGCACAGAACAAAACAATAAAAAAGAGCAGAAACTAATCTGCCCTTGAGGAAATAACCTGAGAGTTTGATGAACGCTTGAAGTTTCTACTGACAGATAAAACCATTTAACTTCATaa                             >  2:90724/1‑250 (MQ=255)                                                                                                                                                                                                                                     aTGTTTTACGTAAAGTCAATTACCGCCAGAGAGGCAAAATGGGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCa       <  1:473958/251‑1 (MQ=255)                                                                                                                                                                                                                                          ttACGTAAAGTCAATTACCGCCAGAGAGGCAAAATGGGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATtctc  >  2:443730/1‑251 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                CCAGGCGTAAATTTTATTTTACCTGGATTTGAATCCGCATAATTTAATGATTCATGTGAATATCCGGCGACATCAATATTCGCCGAACCTATAATTACGACATAATCCTTTTCGCGCATAGCACGTCCTCTGGTAAAAAATTACCAACCCTGTCAATCCACAAGAATATAGATACTGCAAAGACGGTTTAAACATGTGTTCATATTTAAACATGTGCTCATAGTATGCATGTTTTACGTAAAGTAAATTACCGCCAGAGAGGCAAAATGGGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTC  >  NC_000913/2259178‑2259661

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑