Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,667,460	G→A	Q369* (CAG→TAG)	mlc ←	DNA‑binding transcriptional repressor Mlc

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,667,460	0	G	A	100.0%	151.1 / NA	48	Q369* (CAG→TAG)	mlc	DNA‑binding transcriptional repressor Mlc
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (26/22);  total (26/22)

TCACTAACTCCACCGTTATGCTTCACAAATATAAACCAGGAAAATAATTAACCTTGAAAGTCTAAGTTATGCTTTCCTGGCCCAAATTGAGATAGCGCAAATTTTGGTAGAACAGTTAAAAAATGTTAACCCTGCAACAGACGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTAGATCGCCGCGCAATGCCGCCTGACACAATGAGTCCACGGTTAACGGTTGTCCATGTA  >  NC_000913/1667219‑1667696                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                             tCACTAACTCCACCGTTATGCTTCACAAATATAAACCAGGAAAATAATTAACCTTGAAAGTCTAAGTTATGCTTTCCTGGCCCAAATTGAGATAGCGCAAATTTTGGTAGAACAGTTAAAAAATGTTAACCCTGCAACAGACGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTAACTATACGc                                                                                                                                                                                                                                     >  2:213801/1‑251 (MQ=255)      aaCTCCACCGTTATGCTTCACAAATATAAACCAGGAAAATAATTAACCTTGAAAGGCTAAGTTATGCTTTCCTGGCCCAAATTGAGATAGCGCAAATTTTGGTAGAACAGTTAAAAAATGTTAACCCTGCAACAGACGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACGCTCAACGCTGATGTTCTAACTATACGCAgg                                                                                                                                                                                                                                  <  2:205909/249‑1 (MQ=255)          ccACCGTTATGCTTCACAAATATAAACCAGGAAAATAATTAACCTTGAAAGTCTAAGTTATGCTTTCCTGGCCCAAATTGAGATAGCGCAAATTTTGGTAGAACAGTTAAAAAATGTTAACCCTGCAACAGACGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACGCTCAACGCTGATGTGCTAACTATACGCAGGAAgg                                                                                                                                                                                                                              <  1:393650/249‑1 (MQ=255)          ccACCGTTATGCTTCACAAATATAAACCAGGAAAATAATTAACCTTGAAAGTCTAAGGTATGCTTTCCTGGCCCAAATTGAGATAGCGCAAATGTTGGTAGAACAGTTAAAAAATGTTAACCCTGCAACAGACGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAGCGGAGTACTCTCAACGCTGATGTGCTAACTATACGCAGGAAGGGc                                                                                                                                                                                                                            <  2:73859/251‑1 (MQ=255)                  aTGCTTCACAAATATAAACCAGGAAAATAATTAACCTTGAAATTCTAAGTTATGCTTTCCTGGCCCAAATTGAGATAGCGCAAATTTTGGTAGAACAGTTAAAAAATGTTAACCCTGCAACAGACGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTAACTATACGCAGGAAGGGCCTGCTGAc                                                                                                                                                                                                                    <  2:374888/251‑1 (MQ=255)                                 aaaCCAGGAAAATAATTAACCTTGAAAGTCTAAGTTATGCTTTCCTGGCCCAAATTGAGATAGCGCAAATTTTGGTAGAACAGTTAAAAAATGTTAACCCTGCAACAGACGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTAACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTgag                                                                                                                                                                                                      <  1:316917/250‑1 (MQ=255)                                    ccAGGAAAATAATTAACCTTGAAAGTCTAAGTTATGCTTTCCTGGCCCAAATTGAGATAGCGCAAATTTTGGTAGAACAGTTAAAAAATGTTAACCCTGCAACAGACGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTAACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGa                                                                                                                                                                                                  <  1:193809/251‑1 (MQ=255)                                                            gTCTAAGGTATGCTTTCCTGGCCCAAATTGAGATAGCGCAAATTTTGGTAGAACAGTTAAAAAATGTTAACCCTGCAACAGACGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGAGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTAACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGGGGCAATCTGCAGCtt                                                                                                                                                                          >  2:496491/1‑251 (MQ=255)                                                              cTAAGTTATGCTTTCCTGGCCCAAATTGAGATAGCGCAAATTTTGGTAGAACAGTTAAAAAATGTTAACCCTGCAACAGACGAATCAACAAAGAACCGTTATACCTCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTAACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCttt                                                                                                                                                                         >  1:195995/1‑250 (MQ=255)                                                               tAAGTTATGCTTTCCTGGCCCAAATTGAGATAGCGCAAATTTTGGTAGAACAGTTAAAAAATGTTAACCCTGCAACAGACGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTAACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTAc                                                                                                                                                                       >  2:10812/1‑251 (MQ=255)                                                                  gTTATGCTTTCCTGGCCCAAATTGAGATAGCGCAAATTTTGGTAGAACAGTTAAAAAATGTTAACCCTGCAACAGACGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTAACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGcc                                                                                                                                                                              <  2:462880/241‑1 (MQ=255)                                                                  gTTATGCTTTCCTGGCCCAAATTGAGATAGCGCAAATTTTGGTAGAACAGTTAAAAAATGTTAACCCTGCAACAGACGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTAACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGcc                                                                                                                                                                              >  1:462880/1‑241 (MQ=255)                                                                     aTGCTTTCATGGCCCAAATTGAGATAGCGCAAATTTTGGTAGAACAGTTAAAAAATGTTAACCCTGTAACAGACGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGAAGCGCCTGCCATCCAGCCCTGGTTTGAAAACTGAGGACACTCAACGCTGATGGGCTAAATATACGCGGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTCACg                                                                                                                                                                 >  1:22747/1‑251 (MQ=255)                                                                     aTGCCTTCCTGTCCCAAATTGAGATAGCGCAAATTTTGTTAGAACAGTTAAAAAATGTTAACCCTGCAACAGACGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCAGCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTAACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAAc                                                                                                                                                                  <  2:419220/250‑1 (MQ=255)                                                                             cTGGCCCAAATTGAGATAGCGCAAATTTTGGTAGAACAGTTAAAAACTGTTAACCCTGCAACAGACGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTAACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGcc                                                                                                                                                          >  2:385807/1‑250 (MQ=255)                                                                             cTGGCCCAAATTGAGATAGCGCAAATTTTGGTAGAACAGTTAAAAAATGTTAACCCTGCAACAGACGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTAACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGcc                                                                                                                                                          >  1:159019/1‑250 (MQ=255)                                                                               ggCCCAAATTGAGATAGCGCAAATTTTGGTAGAACAGTTAAAAAATGTTAACCCTGCAACAGACGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTAACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAAt                                                                                                                                                       >  1:29621/1‑251 (MQ=255)                                                                                   cAAATTGAGATAGCGCAAATTTTGGTAGAACAGTTAAAAAATGTTAACCCTGCAACAGCCGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGACCTGGTTCGAAACCTGAGTACTCTCAACGCTGATGTGCTACCTATACGCAGGCAGGGCCTGCTGACGGCTGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCCATCAGt                                                                                                                                                   >  2:40431/1‑251 (MQ=255)                                                                                         gagaTAGCGCAAATTTTGGTAGGACAGTTAAAAAATGTTAACCCTGCAACAGACGAATCAACAAAGAACCGTTATACATCGAGTCTTTTACCAGTGCAGCGCCTGCAATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTAACTATACGCAGGCAGGGCCTGCTGACGGCTGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTAttttt                                                                                                                                             >  2:446465/1‑251 (MQ=255)                                                                                                     aTTTTGGTAGAACAGTTAAAAAATGTTAACCCTGCAACAGACGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTAACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGAGAGCCAATCAGTATTTTTTGTGGGTtaaat                                                                                                                                 >  1:398084/1‑251 (MQ=255)                                                                                                        ttGGTAGAACAGTTAAAAAATGTTAACCCTGCAACAGACGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTAACtat                                                                                                                                                                                                                                         >  2:345029/1‑144 (MQ=255)                                                                                                        ttGGTAGAACAGTTAAAAAATGTTAACCCTGCAACAGACGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTAACtat                                                                                                                                                                                                                                         <  1:345029/144‑1 (MQ=255)                                                                                                         tgttAGAACAGTTAAAAAATGTTAACCCTGCAACAGACGAAACAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGGTAGAAAACTGAGTACTCTCAACGCTGATGTGCTAACTATACGCAGGAAGGGCCGGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTtaaataaa                                                                                                                              <  2:415604/247‑1 (MQ=255)                                                                                                            aagttgtcggtagtatccatagcccTGCAACAGACGAATCAACAAAGAACCGTTATACATCGCGTCCTTTACCAGTGCAGCGCCTGCCATCGTGTCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGGGCTAACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGTTATCTGCCGTTTTACTTAACGGTGAGCCAATCAGTATTCTTTGTGGGGTAAATAAATTCa                                                                                                                          <  2:194181/229‑1 (MQ=255)                                                                                                               aaCAGTTAAAAAATGTTAACCCTGCAACAGCCGAATCAACAAAGAACCGTTCTACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCAGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGATAACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGAAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGGGATTTTTGTGGGTTACATAAATTCAcc                                                                                                                        >  2:345808/1‑250 (MQ=255)                                                                                                                  aGTTAAGAAATGTTAACCCTGCAACAGACGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGCTGTGCTAACTATACGCTGGAAGGGCATTCTGACGGATGATGTCTAAGATGCCAGGGACGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATGTTTTGTGGGTTAAATAAAATCACCatg                                                                                                                     >  2:190247/1‑250 (MQ=255)                                                                                                                      aaaaaaTGTTAACCCTGCAACAGACGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTAACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGGGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGc                                                                                                                >  2:59269/1‑251 (MQ=255)                                                                                                                          aaTGTTAACACTGCAACAGACGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGACATAGTGCCCTGGTTAGAAAACTGAGTACTATCAACGCTGATGTGCTAACAATACGCAGGAAGGGCCGGCGGACGGAGGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGGTTTACTTAACGGTGAGCCAATCAGAATTTTTTGTGGGTTAAATAAATTCACCATGAAGGCa                                                                                                               >  2:381056/1‑248 (MQ=255)                                                                                                                                aaCCCTGCAACAGACGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTAACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATgcg                                                                                                       <  1:463103/250‑1 (MQ=255)                                                                                                                                       cAACAGACGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTAACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACAt                                                                                               >  1:191898/1‑251 (MQ=255)                                                                                                                                        aaCAGACGAATCAACAAAGAACCGTTATACATCGCTGCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTAACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATg                                                                                              <  1:470910/251‑1 (MQ=255)                                                                                                                                                    aacaaAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTAACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCAcacc                                                                                  >  2:20144/1‑248 (MQ=255)                                                                                                                                                     acaaAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTAACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCg                                                                                 <  1:10812/251‑1 (MQ=255)                                                                                                                                                       aaaGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTAGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTAACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGt                                                                               >  1:221436/1‑251 (MQ=255)                                                                                                                                                             ccGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTAACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATg                                                                                              >  2:316619/1‑230 (MQ=255)                                                                                                                                                             ccGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTAACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATg                                                                                              <  1:316619/230‑1 (MQ=255)                                                                                                                                                                                   ccAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTAACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAAGCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTAGATcgccg                                                   <  1:246956/251‑1 (MQ=255)                                                                                                                                                                                    cAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTCCTCTCAACGCTGATGTGCTAACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTtaaata                                                                                                                                >  1:183372/1‑173 (MQ=255)                                                                                                                                                                                    cAGTGCAGCGCCTGCCACCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTAACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACATAACGGTGAGCCAATCAGTATTTTTTGTGGGTtaaata                                                                                                                                <  2:183372/173‑1 (MQ=255)                                                                                                                                                                                            cgcCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTAACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACTGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTAGATCGCCGCGCAATgcc                                          <  1:248151/251‑1 (MQ=255)                                                                                                                                                                                             gcCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTAACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTGCTTAACTGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTAGATCGCCGCGCAATgccg                                         <  1:492234/251‑1 (MQ=255)                                                                                                                                                                                                tGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTAACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTCCCAGTAGATCGCCGCGCAATGCCGCCt                                      >  1:167330/1‑251 (MQ=255)                                                                                                                                                                                                       gTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTAACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAACTAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCACCCACCCCGGTAATGATGTCTTTTGCCAGTAGATCGCCGCGCAATGCCCCCTGAcaca                                >  2:15630/1‑250 (MQ=255)                                                                                                                                                                                                           ccTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTAACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGTTAATGATGTCTTTTGCCAGTAGATCGCCGCGCAATGCCGCCTGACACAATGAg                           <  1:246499/251‑1 (MQ=255)                                                                                                                                                                                                                      accTGAGTACTCTCAACGCTGATGTGCTAACTATACGCAGGAAGGGCCTGCTGACGGATGCTGGCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCTCCATGATGGCAAGAATGCGCCCGACATGCGCGCCCGCCCCGGTAATGATGTCTTTTGCCAGTAGATCGCCGCGCAATGCCGCCTGACACAATGAGTCCACGGTTaa                <  1:306704/249‑1 (MQ=255)                                                                                                                                                                                                                              aCTCTCAACGCTGATGTGCTAACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTAGATCGCCGCGCAATGCCGCCTGACACAATGAGTCCACGGTTAACGGTTGTc        <  1:323863/251‑1 (MQ=255)                                                                                                                                                                                                                                  tcAACGCTGATGTGCTAACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTAGATCGCCGCGCAATGCCGCCTGACACAATGAGTCCACGGTTAACGGTTGTCCATg    >  1:102428/1‑251 (MQ=255)                                                                                                                                                                                                                                     aCGCTGATGGGCTAACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGGGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATTGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTAGATCGCCGCCCACTGACGCCTGACACAATGAGCCCCCGGTTAACGGGTGACCATgaa  >  2:402218/1‑248 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                             TCACTAACTCCACCGTTATGCTTCACAAATATAAACCAGGAAAATAATTAACCTTGAAAGTCTAAGTTATGCTTTCCTGGCCCAAATTGAGATAGCGCAAATTTTGGTAGAACAGTTAAAAAATGTTAACCCTGCAACAGACGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTAGATCGCCGCGCAATGCCGCCTGACACAATGAGTCCACGGTTAACGGTTGTCCATGTA  >  NC_000913/1667219‑1667696

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑