Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,490,095	G→A	intergenic (+424/‑807)	aldA → / → cybB	aldehyde dehydrogenase A/superoxide oxidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,490,095	0	G	A	96.3%	73.3 / ‑4.0	27	intergenic (+424/‑807)	aldA/cybB	aldehyde dehydrogenase A/superoxide oxidase
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (11/15);  total (11/16)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.52e-01

TGCCAATGATATCGGAAGAGACTATTTCTTCATCGGTATAACCAAATGACTCGTTATTGGTGGTTGCTTGTTTAAGTGCGTTATTCACCTCTTCGGCAGTCACTTTTTTCCGAGAATCGATACCAGTTCAGTGACCGAACCTGTTTTCACCGGCACGCGTTGCGCATGACCTTTCAGTTTGCCGCTCA‑GTTCCGGGATCACCAGACCAATGGCTTTTGCCGCCCCCGTAGTGTGGGGAATGATATTTTCTGCCGCTGCGCGTGAAGCACGTAAATCTTTACCACGCGGGCCATCCACCAGTGACTGGGTGCCAGTATAGGCATGAATGGTCGTCATCGTGCCGACTTCTATCCCGAAACTGTCATGCAAGGCTTTGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACGCCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGACGTTATAAAC  >  NC_000913/1489862‑1490326                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                        tGCCAATGATATCGGAAGAGACTATTTCTTCATCGGTATAACCAAATGACTCGTTATTGGTGGTTGCTTGTTTAAGTGCGTTATTCACCTCTTCGGCAGTCACTTTTTTCCGAGAATCGATACCAGTTCAGTGACCGAACCTGTTTTCACCGGCACGCGTTGCGCATGACCTTTCAGTTTGCCGCTCA‑GTTCCGGGATCACCAGACCAATGGCTTTTGCCGCCCCCGTAGTGTAGGGCATGATATTTTCTg                                                                                                                                                                                                                        >  1:364738/1‑251 (MQ=255)  gCCAATGATATCGGAAGAGACTATTTCTTCATCGGTATAACCAAATGACTCGTTATTGGTGGTTGCTTGTTTAAGTGCGTTATTCACCTCTTCGGCAGTCACTTTTTTCCGAGAATCGATACCAGTTCAGTGACCGAACCTGTTTTCACCGGCACGCGTTGCGCATGACCTTTCAGTTTGCCGCTCA‑GTTCCGGGATCACCAGACCAATGGCTTTTGCCGCCCCCGTAGTGTAGGGAATGATATTTTCt                                                                                                                                                                                                                         <  2:215126/249‑1 (MQ=255)       tGATATCGGCAGAGACTATTTCTTCATCGGTATAACCAAATGACTCGTTATTGGTGGTTGCTTGTTTAAGTGCGTTATTCACCTCTTCGGCAGTCACTTTTTTCCGAGAATCGATACCAGTTCAGTGACCGACCCTGTTTTCACCGGCACGCGTTGCGCATGACCTTTCAGTTTGCCGCTCA‑GTTCCGGGATCACCAGACCAATGGCTTTTGCCGCCCCCATAGTGTAGGGAATGATATTTTCTGCCGCTg                                                                                                                                                                                                                  >  1:83455/1‑251 (MQ=255)                aaGAGACTATTTCTTCATCGGAATAACCAAATGACTCGTTATTGGTGGTTGCATGTTTAAGTGCGTTATTCACCTCTTCGGCAGTCAATTTTTTCGGAGACTCGATACCAGTTCAGTGACCGAACCTGTTTTCAACGGCACGCGTTGCGCATGACCTTTCAGTTTGCCGCTCA‑GTTCCGGGATCACCAGACCAAGGGCTTTTGCCTCCCACGTAGATCAGGGAATGATATTTTCTGCCGCCGTGCGTGa                                                                                                                                                                                                           >  2:35113/1‑249 (MQ=255)                            cttcATCGGTATAACCAAATGACTCGTTATTGGTGGTTGCTTGTTTAAGTGCGTTATTCACCTCTTCGGCAGTCACTTTTTTCCGAGAATCGATACCAGTTCAGTGACCGAACCTGTTTTCACCGGCACGCGTTGCGCATGACCTTTCAGTTTGCCGCTCA‑GTTCCGGGATCACCAGACCAATGGCTTTTGCCGCCCCCGTAGTGTAGGGAATGATATTTTCTGCCGCTGCGCGGGAAGCACGTAAATCtt                                                                                                                                                                                             >  2:153442/1‑251 (MQ=255)                              tcATCGGTATAACCAAATGACTCGTTATTGGTGGTTGCTTGTTTAAGTGCGTTATTCACCTCTTCGGCAGTCACTTTTTTCCGAGAATCGATTCCAGTTCAGTGACCGAACCTGTTTTCACCGGCACGCGTTGCGCATGACCTTTCAGTTTGCCGCTCA‑GTTCCGGGATCACCAGACCAATGGCTTTTGCCGCCCCCGTAGTGTAGGGAATGATATTTTCTGCCGCTGCGCGTGAAGCACGTAAATCt                                                                                                                                                                                              <  2:353823/248‑1 (MQ=255)                                        aaCCAAATGACTCGTTATTGGTGGTTGCTTGTTTAAGTGCGTTATTCACCTCTTCGGCAGTCACTTTTTTCCGAGAATCGATACCAGTTCAGTGACCGAACCTGTTTTCACCGGCACGCGTTGCGCATGACCTTTCAGTTTGCCGCTCA‑GTTCCGGGATCACCAGACCAATGGCTTTTGCCGCCCCCGTAGTGTGGGGAATGATATTTTCTGCCGCTGCGCGTGAAGCACGTAAATCTTTACCACGCggg                                                                                                                                                                                  <  1:173836/250‑1 (MQ=255)                                         aCCAAATGACTCGTTATTGGTGGTTGCTTGTTTAAGTGCGTTATTCACCTCTTCGGCAGTCACTTTTTTCCGAGAATCGATACCAGTTCAGTGACCGAACCTGTTTTCACCGGCACGCGTTGCGCATGACCTTTCAGTTTGCCGCTCA‑GTTCCGGGATCACCAGACCAATGGCTTTTGCCGCCCCCGTAGTGTAGGGAATGATATTTTCTGCCGCTGCGCGTGAAGCACGTAAATCTTTACCACGCGGGcc                                                                                                                                                                                >  1:122923/1‑251 (MQ=255)                                          ccAAATGACTCTTTATTGGTGGTTGCGTGTTTAAGTGCGTTATTCACCTCTTCGGCAGTCACTTTTTTCCGAGAATCGATACCAGTTCAGTGACCGAACCTGTTTTCACCGGCACGCGTTGCGCATGACCTTTCAGTTTGCCGCTCA‑GTTCCGGGATCACCAGACCAATGGCTTTTGCCGCCCCCGTAGTGTAGGGAATGATATTTTCTGCCGCTGCGCGTGAAGCACGTAAATCTTTACCACGCGGGCCa                                                                                                                                                                               <  2:465100/251‑1 (MQ=255)                                           cAAATGACTCGTTATTGGTGGTTGCTTGTTTAAGTGCGTTATTCACCTCTTCGGCAGTCACTTTTTTCCGAGAATCGATACCAGTTCAGTGACCGAACCTGTTTTCACCGGCACGCGTTGCGCATGACCTTTCAGTTTGCCGCTCA‑GTTCCGGGATCACCAGACCAATGGCTTTTGCCGCCCCCGTAGTGTAGGGAATGATATTTTCTGCCGCTGCGCGTGAAGCACGTAAATCTTTACCACGCGGGCCAt                                                                                                                                                                              >  2:235996/1‑251 (MQ=255)                                               tGACTCGTTATTGGTGGTTGCTTGTTTAAGTGCGTTATTCACCTCTTCGGCAGTCACTTTTTTCCGAGAATCGATACCAGTTCAGTGACCGAACCTGTTTTCACCGGCACGCGTTGCGCATGACCTTTCAGTTTGCCGCTCA‑GTTCCGGGATCACCAGACCAATGGCTTTTGCCGCCCCCGTAGTGTAGGGAATGATATTTTCTGCCGCTGCGCGTGAAGCACGTAAATCTTTACCACGCGGGCCCTccac                                                                                                                                                                          >  2:11474/1‑251 (MQ=255)                                                                         tAAGTGTGTTATTCCCCTCTTCGGCAGTTACTTTTTTCCGAGAATCGATACCAGGTCAGTGACCGAACCTGTTTTCACCGGCACGCGTTGCGCATGACCTTTCAGTTTGCCGCTCA‑GTTCCGGGATCACCAGACCAATGGCTTTTGCCGCCCCCGTAGTGTAGGGAATGATATTTTCTGCCGCTGCGCGTGAAGCACGTAAATCTTTACCACGCGGGCCATCCACCAGTGACTGGGTGCCAGTATAGGCa                                                                                                                                                 <  1:414084/250‑1 (MQ=255)                                                                               cGTTATTCACCTCTTCGGCAGTCACTTTTTTCCGAGAATCGATACCATTTCAGTGACCGAACCTGTTTTCACCGGCACGCGTTGCGCATGACCTTTCAGTTTGCCGCTCA‑GTTCCGGGATAACCAGACCAATGGCTTTTGCCGCCCCCGTAGTGTAGGGAATGATATTTTCTGCCGCTGCGCGTGAAGCACGTCAATCTTTACCACGCGGGCCATCCACCAGTGACTGGGTGCCAGTATAAGCATGAATgg                                                                                                                                          >  1:435772/1‑251 (MQ=255)                                                                                        ccTCTTCGGCAGTCTCTTTATCCCGATAAGCGATACCCTTTCAGTGTCCGAACCTGTTGTACCCGGCCAGCGTTTCGCATGACCTTTCAGTTCGCCGC‑CACGTTCCGGGATCACCAGACCAATGGCTCTTGCCGCCCCCGTAGTGTAGGGAATGATATTTTCTTCCGCTGCGCGTGAAGCACGTAAATCTTTACCACGCGGGCCATCCACCAGTGACTGGGTGCCAGTATATGCATGAATGGTCGTCagcg                                                                                                                                 <  2:55923/251‑4 (MQ=255)                                                                                               ggCAGTCACTTTTTTCCGAGAATCGATACCAGTTCAGTGACCGAACCTGTTTTCACCGGCACGCGTTGCGCATGACCTTTCAGTTTGCCGCTCA‑GTTCCGGGATCACCAGACCAATGGCTTTTGCCGCCCCCGTAGTGTAGGGAATGATATTTTCTGCCGCTGCGCGTGAAg                                                                                                                                                                                                         >  2:174482/1‑172 (MQ=255)                                                                                               ggCAGTCACTTTTTTCCGAGAATCGATACCAGTTCAGTGACCGAACCTGTTTTCACCGGCACGCGTTGCGCATGACCTTTCAGTTTGCCGCTCA‑GTTCCGGGATCACCAGACCAATGGCTTTTGCCGCCCCCGTAGTGTAGGGAATGATATTTTCTGCCGCTGCGCGTGAAg                                                                                                                                                                                                         <  1:174482/172‑1 (MQ=255)                                                                                                              ccGAGAATCGATACCAGTTCAGTGACCGAACCTGTTTTCACCGGCACGCGTTGCGCATGACCTTTCAGTTTGCCGCTCA‑GTTCCGGGATCACCAGACCAATGGCTTTTGCCGCCCCCGTAGTGTAGGGAATGATATTTTCTGCCGCTGCGCGTGAAGCACGTAAATCTTTACCACGCGGGCCATCCACCAGTGACTGGGTGCCAGTATAGGCATGAATGGTCGTCATCGTGCCGACTTCTATCCCGAAACt                                                                                                           <  1:235996/251‑1 (MQ=255)                                                                                                              ccGAGAAGCGATACCAGTTCAGTGACCGAACCTGTTTTCACCGGCACGCGTTGCGCATGACCTATCAGTTTGCCGCTCA‑GTTCCGGGATCACCAGACCAATGGCTTTTGCCGCCCCAGAAGGGTAGGGCCTGATATTTTCTGCCGCTGCGCGTGAAGCACGAAAATCGTTACCACGCGAGCCATCCACAAGTGCCTGGGTGCCAGGATCGGCATGAATGGTCGTCATCTTTCCGACTTCTATCCCTAAACt                                                                                                           >  2:267386/1‑251 (MQ=255)                                                                                                                          tccAGTTCAGTTACCGAACCTTTTGTCACCGGCCCGCGTTGCGCATGACCTGTCAGTTTGCTGCTCA‑GTTCCGGGATCACCAGACCAATGGCTTTTGCCGCCCCCGTAGTGTAGGGAATTATATTTTCTGCCGCTGCGCGTGAAGCACTTAAATCTTTACCACGCGGGCCATCCTCCAGTGACTGGGTGCCAGTATAGGCATGAATGGTCGTCATCGTGCCGACTTCTATCCCGAAACTGTCAGGCAAgg                                                                                                <  2:432720/249‑1 (MQ=255)                                                                                                                              gTTCAGTGACCGAACCTGTTTTCACCGGCACGCGTTGCGCATGACCTTTCAGTTTGCCGCTCA‑GTTCCGGGATCACCAGACCAATGGCTTTTGCCGCCCCCGTAGTGTAGGGAATGATATTTTCTGCCGCTGCGCGTGAAGCACGTAAATCTTTACCACGCGGGCCATCCACCAGTGACTGGGTGCCAGTATAGGCATGAATGGTCGTCATCGTGCCGACTTCTATCCCGAAACTGTCATGCAAGGCTTTg                                                                                           <  2:164883/251‑1 (MQ=255)                                                                                                                                   gTGACCGAACCTGTTTTCACCGGCACGCGTTGCGCATGACCTTTCAGTTTGCCGCTCA‑GTTCCGGGATCACCAGACCAATGGCTTTTGCCGCCCCCGTAGTGTAGGGAATGATATTTTCTGCCGCTGCGCGTGAAGCACGTAAATCTTTACCACGCGGGCCATCCACCAGTGACTGGGTGCCAGTATAGGCATGAATGGTCGTCATCGTGCCGACTTCTATCCCGAAACTGTCATGCAAGGCTTTGGCCAt                                                                                      <  1:153442/251‑1 (MQ=255)                                                                                                                                                        ggCACGCGTTGCGCATGACCTTTCAGTTTGCCGCTCA‑GTTCCGGGATCACCAGACCAATGGCTTTTGCCGCCCCCGTAGTGTAGGGAATGATATTTTCTGCCGCTGCGCGTGAAGCACGTAAATCTTTACCACGCGGGCCATCCACCAGTGACTGGGTGCCAGTATAGGCATGAATGGTCGTCATCGTGCCGACTTCTATCCCGAAACTGTCATGCAAGGCTTTGGCCATCGGCGCAAGACAGTTAgtggt                                                                 <  1:37843/251‑1 (MQ=255)                                                                                                                                                            cgcgTTGCGCATGACCTTTCAGTTTGCCGCTCA‑GTTCCGGGATCACCAGACCAATGGCTTTTGCCGCCCCCGTAGTGTAGGGAATGATATTTTCTGCCGCTGCGCGTGAAGCACGTAAATCTTTACCACGCGGGCCATCCACCAGTGACTGGGTGCCAGTATAGGCATGAATGGTCGTCATCGTGCCGACTTCTATCCCGAAACTGTCATGCAAGGCTTTGGCCATCGGCGCAAGACAGTTAGTGGTGCAt                                                             >  1:155476/1‑251 (MQ=255)                                                                                                                                                                                      ccGCTCA‑GTTCCGGGATCACCAGACCAATGGTTTTTGCCGCCCCCGTATTGTAGGGAATGATATTTACTGCCGCTGCGCGTGAATCACGTAAATCTTTACCACGCGGGCCATCCACCAGTGACTGGGTGCCAGTATAGGCATGAATGGTCGGCATCGTGCCGACTTCTATCCCGAAACTGTCATGCAAGGCTTTGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACGCCACGGAAACAATGGTGTCGtt                                   <  2:364738/251‑1 (MQ=255)                                                                                                                                                                                                                   gggCTTTTGCCGCCCCCGTAGTGTAGGGAATGATATTTTCTGCCGCTGCGCGTGAAGCACGTAAATCTTTACCACGCGGGCCATCCACCAGTGACTGGGTGCCAGTATAGGCATGAATGGTCGTCATCGTGCCGACTTCTATCCCGAAACTGTCATGCAAGGCTTTGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACGCCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGACGTta       <  2:155476/250‑1 (MQ=255)                                                                                                                                                                                                                    ggCTTTTGCCGCCCCCGTAGTGTAGTGAATGATATTTTCTGCCGCGGCGCGTGAAGCACGTAAATCTTTACCACGCGGGCCATCCACCAGTGACTGGGTGCCAGTATAGGCATGAATGGTCGTCATCGTGCCGACTTCTATCCCGAAACTGTCATGCAAGGCTTTGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACGCCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGACGTta       <  1:255004/250‑1 (MQ=255)                                                                                                                                                                                                                         ttGCCGCCCCCGGAGTGTAGGGAATGATAGTTTCTGCCGCTGCGCGTGAAGCACGTAAATCTTTACCACGCGGGCCATCCACCAGTGACTGGGTGCCAGTATAGGCATGAATGGTCGTCATCGTGCCGACTTCTATCCCTAAACTGTCATGCAAGGCTTTGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACGCCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGACGTTATAAAc  <  1:481721/250‑1 (MQ=255)                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                        TGCCAATGATATCGGAAGAGACTATTTCTTCATCGGTATAACCAAATGACTCGTTATTGGTGGTTGCTTGTTTAAGTGCGTTATTCACCTCTTCGGCAGTCACTTTTTTCCGAGAATCGATACCAGTTCAGTGACCGAACCTGTTTTCACCGGCACGCGTTGCGCATGACCTTTCAGTTTGCCGCTCA‑GTTCCGGGATCACCAGACCAATGGCTTTTGCCGCCCCCGTAGTGTGGGGAATGATATTTTCTGCCGCTGCGCGTGAAGCACGTAAATCTTTACCACGCGGGCCATCCACCAGTGACTGGGTGCCAGTATAGGCATGAATGGTCGTCATCGTGCCGACTTCTATCCCGAAACTGTCATGCAAGGCTTTGGCCATCGGCGCAAGACAGTTAGTGGTGCATGACGCCACGGAAACAATGGTGTCGTTGCCATCCAGAGTGTCGTCATTGACGTTATAAAC  >  NC_000913/1489862‑1490326

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑